CN106699329A - Chrysanthemum indicum polysaccharide-containing foliar fertilizer composition and application thereof - Google Patents
Chrysanthemum indicum polysaccharide-containing foliar fertilizer composition and application thereof Download PDFInfo
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Abstract
The invention discloses a chrysanthemum indicum polysaccharide-containing foliar fertilizer composition. The chrysanthemum indicum polysaccharide-containing foliar fertilizer composition is characterized by comprising chrysanthemum indicum polysaccharide capable of promoting plant growth, a chrysanthemum indicum extract and a trace element, wherein chrysanthemum indicum polysaccharide is a carbohydrate extracted from plant chrysanthemum indicum and is a copolymer of rhamnose, arabinose, xylose, mannose, glucose, galactose and fructose, and the content thereof reaches 99%; the trace element comprises at least one of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, iron, manganese, copper, zinc and molybdenum; the chrysanthemum indicum extract is a water extract or an ethanol extract extracted from the chrysanthemum indicum; the chrysanthemum indicum polysaccharide accounts for 1-10% of the total weight of the chrysanthemum indicum polysaccharide-containing foliar fertilizer composition; the chrysanthemum indicum or chrysanthemum indicum stem and leaf extract accounts for 0-50% of the total weight of the chrysanthemum indicum polysaccharide-containing foliar fertilizer composition; a trace element foliar fertilizer accounts for 5-50% of the total weight of the chrysanthemum indicum polysaccharide-containing foliar fertilizer composition, and the trace element comprises at least one of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, iron, manganese, copper, zinc and molybdenum. The chrysanthemum indicum polysaccharide-containing foliar fertilizer composition is applied to Chinese herbs and cash crops, especially root-tuber crops, and can not only increase the yield, but also reduce diseases and pests.
Description
Technical field
The present invention relates to agriculture field, the composition and said composition of a kind of utilization healthy food material production foliar fertilizer are more particularly related in Chinese medicine
Material, the application of industrial crops.
Technical background
Due to increasingly serious residues of pesticides and excessively using chemical agricultural fertilizer for crop, soil, the adverse effect that water source is caused, plant source agricultural fertilizer gesture
One of solution must be turned into, the consideration for food security is particularly in, containment chemical pesticide is also all gradually recognized from country to agribusiness
With the necessity of agricultural fertilizer usage quantity.Blade face fertilizer product with active substance of plant as principal component is the new direction of foliar fertilizer industry development, meets the modern times
The requirement of the ecological agriculture, and have the advantages that wide material sources, exploitation less investment, market development space are big, will be occupied in following plant source agricultural fertilizer
An important seat.
Foliar fertilizer is will be nontoxic and containing various nutritional ingredients, is sprayed on the blade face of plant by doses and concentration, directly feeds crop and supports
Point, it is a kind of method of foliage fertilization.It was verified that blade face can directly absorb and utilize, can rapidly improve the nutrition condition of plant, promote plant
Grow, improve product quality.At present, plant growth regulator class material is added in the foliar fertilizer of part, is agriculturally being used both at home and abroad
Conditioning agent be mainly chemical synthesis, such as gibberellin, heteroauxin, such material has a strong impact on human health into human body.At present, with plant
Thing active component is poor for the fertilizer efficiency that the blade face fertilizer product of principal component overcomes traditional element fertilizer, and penetration is weak, the defect such as crop absorption difference, with wide
Application prospect.Foliar fertilizer of the present invention, with plant source mother chrysanthemum polysaccharide as raw material, for Chinese medicine, industrial crops etc., especially tubers
Chinese medicine, can promote various crops to increase stem, and can effectively prevent various diseases, crop quality be improved, to soil, crop without residual hazard.It is exploitation
Production pollution-free food, non agricultural chemical residuum medicinal material have established strong fertilizer basis, and huge ecology and social effect will be produced to development green agriculture.
Patent report is had no containing the foliage fertilizer composition with mother chrysanthemum polysaccharide as active component.
The content of the invention
It is an object of the invention to：A kind of foliage fertilizer composition containing plant polyose, plant extracts is provided, wherein, it is many that said composition contains mother chrysanthemum
Sugar, mother chrysanthemum extract and various microelement foliage fertilizers, element foliar fertilizer is introduced by the mother chrysanthemum polysaccharide with inducing plant resistance and promotion root growth
In, with promotion plant root growth and nutrient absorption effect, and disease generation can be suppressed.
To achieve these goals, the technical scheme is that：A kind of foliage fertilizer composition containing mother chrysanthemum polysaccharide, wherein mother chrysanthemum polyoses content are provided
For the 1%-10% of composition total weight, mother chrysanthemum or mother chrysanthemum stem-leaf extract are the 0%-50% of composition total weight, and the content of element foliar fertilizer is combination
The 5%-50% of thing gross weight, element includes nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, boron, Tie, Manganese, copper, zinc, molybdenum one kind at least within, described
After foliage fertilizer composition is through dilution, in crop bud stage foliage-spray, spray within 7-10 days 1 time, continuously spray 2-10 times.
Said composition also contains one or more of the material being selected from the group：Potassium sorbate, dehydroactic acid sodium, propylparaben, sodium lactate,
Water.
Said composition prepares the formulation being selected from the group：Wettable powder, soluble powder, effervescent tablet, granule, micro-capsule, suspending agent, missible oil,
Water and milk.
Described mother chrysanthemum extract be from feverfew mother chrysanthemum (Chrysanthemum indicum) include mother chrysanthemum (Chrysanthemum indicum L.),
The herb of HERBA CHRYSANTHEMI LAVANDULAEFOLII (C.boreale Mak.) or lavandulaleaf dendranthema herb (C.lavandulaefolium (Fisch.) Mak.) etc. includes rhizome and is extracted in spending
The water or ethanol extract for arriving.
Described mother chrysanthemum polysaccharide is that the carbohydrate for obtaining is extracted from plant mother chrysanthemum, is by rhamnose, arabinose, xylose, mannose, Portugal
Grape sugar, galactolipin, the copolymer of fructose composition, content is up to 99%.
Said composition is applied to Chinese medicine, industrial crops.Wherein, yield can be not only improved to root crop can also reduce disease.
The method of said composition application is that foliage-spray is carried out after this product is diluted with water during crop-planting.
It is an advantage of the invention that：
1st, will have induction plant disease-resistant and promote root growth mother chrysanthemum polysaccharide be introduced into element foliar fertilizer, with promote root system of plant growth with
The effect of nutrient absorption, and prevention soil-borne disease is had certain effect, with double effects, can partly substitute chemistry
The use of plant growth regulator, reduces chemical residual, solves food, the safety problem of Chinese medicine.
2nd, the present invention takes full advantage of the synergistic function of various active active principle in mother chrysanthemum with mother chrysanthemum extract solution as solvent, improves plant pair
The repellence of infectious disease, meanwhile, yield and quality is also significantly improved.
3rd, main active trace element of the invention, mother chrysanthemum polysaccharide, mother chrysanthemum extract, wide material sources are cheap, are suitable for extensive
Promote.
4th, foliage fertilizer composition usage amount of the invention is few, and efficiency time is long, and will not produce poisoning.
Brief description of the drawings
The monose composition GC spectrograms of Fig. 1 mother chrysanthemum polysaccharide
Fig. 2 monose standard items GC spectrograms (glucose, xylose, galactolipin, arabinose, rhamnose, fructose, mannose and fucose)
Specific embodiment
With reference to preferred embodiment, the present invention is further illustrated, but the present invention is never limited to following embodiments.
Embodiment 1
The foliage fertilizer composition containing mother chrysanthemum polysaccharide of the present embodiment adds solvent, auxiliary agent to be configured to aqua, missible oil, soluble liquid, suspending agent,
Add common carrier, auxiliary agent to be made into pulvis, soluble powder, wettable powder, granule, tablet etc., the present embodiment by taking aqua as an example,
But present invention is not limited to this：
Take 1 part of mother chrysanthemum polysaccharide, 10 times of mother chrysanthemum cauline leaf Aqueous extracts, 100 parts of dilution, 10 parts of potassium dihydrogen phosphate, 2 parts of ammonium molybdate, copper sulphate
0.5 part, Tween-80 accounts for the 0.5%wt of total amount.
Preparation method step is as follows：
(1) prepared by mother chrysanthemum polysaccharide：Crushed after mother chrysanthemum drying, weigh mother chrysanthemum powder it is appropriate in the aqueous solution (w/v, 1: 90 DEG C of extraction 3h in 10),
Merging filtrate twice is extracted repeatedly, after standing 24h, filtrate is collected after vacuum filtration, be condensed into medicinal extract shape.Thick many candies are obtained using water extraction and alcohol precipitation method.
With sevag method removing proteins, the mother chrysanthemum polysaccharide sterling (being determined using Phenol-sulphate acid method) that purity is 99% is obtained.Analyzed using GC after derivatization, its list
Sugar composition is rhamnose, the copolymer of arabinose, xylose, mannose, glucose, galactolipin, fructose composition.
(2) mother chrysanthemum stem-leaf extract：Take dry mother chrysanthemum cauline leaf powder (cross 40 mesh sieves), by solid-to-liquid ratio (W/V, 1: 10) add water, 90
DEG C extract 3 hours, stand 24h after, after vacuum filtration mother chrysanthemum extract solution stoste.
(3) the mother chrysanthemum stoste of step 2 is diluted with water 100 times, is added in reactor, then stirred to addition ammonium molybdate, copper sulphate in reactor
Dissolving, adds chelator acid citrate, and temperature adjustment is reacted 0.5 hour to 80 DEG C, is cooled to room temperature, to addition mother chrysanthemum polysaccharide, di(2-ethylhexyl)phosphate in reactor
Hydrogen potassium, dissolving, stirs and evenly mixs, and suction filtration, filtrate is standby.
(4) filtrate of step 3 is placed in finished product kettle, adds surface active agent tween -80, be well mixed, both obtain the leaf of mother chrysanthemum polysaccharide composition
Sourdough product.
The method that its secondary and micro-element fertilizers is pressed in NY1428 annexes is determined.
Wherein mother chrysanthemum purity of polysaccharide uses colorimetric method for determining.
Wherein the monose composition of mother chrysanthemum polysaccharide is using GC analyses after derivatization.Chromatographic condition is：GC conditions：Gas chromatograph model
Aglient GC-7890B, HP-5 (0.25 μm of 30m × 320 μ m) fused-silica capillary column, flame ionization ditector (FID).Detection
260 DEG C of device temperature, split ratio 25: 1, sample size 1 μ L, hydrogen flow rate 30mL, air velocity 400mL.Temperature programming：125 DEG C of initial temperature
, 128 DEG C are risen to 1 DEG C/min, 5min is kept, 129 DEG C first are risen to 0.1 DEG C/min afterwards, then 140 DEG C are risen to 1 DEG C/min,
Finally 280 DEG C are risen to 5 DEG C/min.Mother chrysanthemum polysaccharide monose composition GC chromatograms are shown in Fig. 1, are compared with monose standard items, and mother chrysanthemum polysaccharide is by mouse
The heteroglycan that Lee's sugar, arabinose, xylose, mannose, glucose, galactolipin, fructose are constituted.
Foliage fertilizer composition application process is obtained in embodiment 1：The foliage fertilizer composition is diluted 10-2000 times, in ratio of rhizome medicinal material, warp
In the seedling stage of Ji crop, per spraying within 7-10 days 1 time, continuously spray 2-10 times.
Embodiment 2 improves bighead atractylodes rhizome yield, reduces bighead atractylodes rhizome soil-borne disease using this foliage fertilizer composition
Start to spray the product in the seedling stage of bighead atractylodes rhizome growth, the reduction of bighead atractylodes rhizome disease incidence of soil-borne disease can be made, while realizing the purpose of volume increase.
Experiment sets following treatment：(1) the fresh water spraying bighead atractylodes rhizome (CK) is only used；(2) Bravo and Routine Management are sprayed；(3) by described in embodiment 1
Foliage fertilizer composition dilutes 1000 times, sprays.Totally 3 groups for the treatment of, often treatment is weighed 3 times.Other cultivation management measures are identical.
Test is carried out in January, 2014-October on Zhejiang A ＆ F University Pingshan Mountain farm, is 6m per plot area2.In January, 15 is by seed soaking
The Atractylodes macrocephala seed of reason sows the artificial weeding during experimental plot, experiment, and other any herbicides are not used, and dispenser is applied using conventional foliar spray method
The medicine time is April 15, April 25 and May 3, totally three times.It is August 20 when the state of an illness is counted.Observe the onset state of the bighead atractylodes rhizome, root
According to severity Scaling standard, disease index is calculated.Meanwhile, in the picking time of the late October bighead atractylodes rhizome in 2014, bighead atractylodes rhizome total output is counted, calculate and increase
Effect is produced, 1 is the results are shown in Table.
The foliage fertilizer composition of the present invention of table 1 is to the growth of the field bighead atractylodes rhizome, the influence of incidence
Note：Data are the average value ± standard error of three repeating groups, and the significant difference in P=0.05 levels is represented with lowercase after column data is different, and capitalization is different to represent that difference is extremely notable in P=0.01 levels.
Embodiment 3
The foliage fertilizer composition containing mother chrysanthemum polysaccharide of the present embodiment adds solvent, auxiliary agent to be configured to aqua, missible oil, soluble liquid, suspending agent,
Add common carrier, auxiliary agent to be made into pulvis, soluble powder, wettable powder, granule, tablet etc., the present embodiment by taking aqua as an example,
But present invention is not limited to this：
Take 1 part of mother chrysanthemum polysaccharide, 100 parts of water, 10 parts of potassium dihydrogen phosphate, 2 parts of ammonium molybdate, 0.5 part of copper sulphate, Tween-80 accounts for total amount
0.3%wt.
Preparation method step is as follows：
(1) prepared by mother chrysanthemum polysaccharide：Crushed after mother chrysanthemum drying, weigh mother chrysanthemum powder it is appropriate in the aqueous solution (w/v, 1: 90 DEG C of extraction 3h in 10),
Merging filtrate twice is extracted repeatedly, after standing 24h, filtrate is collected after vacuum filtration, be condensed into medicinal extract shape.Thick many candies are obtained using water extraction and alcohol precipitation method.
With sevag method removing proteins, the mother chrysanthemum polysaccharide sterling (being determined using Phenol-sulphate acid method) that purity is 99% is obtained.Analyzed using GC after derivatization, its list
Sugar composition is rhamnose, the copolymer of arabinose, xylose, mannose, glucose, galactolipin, fructose composition.
(water, ammonium molybdate, copper sulphate stirring and dissolving are added in 2 reactors, chelator acid citrate is added, temperature adjustment are reacted 0.5 hour to 80 DEG C,
Room temperature is cooled to, to mother chrysanthemum polysaccharide, potassium dihydrogen phosphate is added in reactor, dissolving is stirred and evenly mixed, and suction filtration, filtrate is standby.
(4) filtrate of step 3 is placed in finished product kettle, adds surface active agent tween -80, be well mixed, both obtain the leaf of mother chrysanthemum polysaccharide composition
Leaven composition.
The method that its secondary and micro-element fertilizers is pressed in NY1428 annexes is determined.
Wherein mother chrysanthemum purity of polysaccharide uses colorimetric method for determining.
Wherein the monose composition of mother chrysanthemum polysaccharide is using GC analyses after derivatization.Chromatographic condition is：GC conditions：Gas chromatograph model
Aglient GC-7890B, HP-5 (0.25 μm of 30m × 320 μ m) fused-silica capillary column, flame ionization ditector (FID).Detection
260 DEG C of device temperature, split ratio 25: 1, sample size 1 μ L, hydrogen flow rate 30mL, air velocity 400mL.Temperature programming：125 DEG C of initial temperature
, 128 DEG C are risen to 1 DEG C/min, 5min is kept, 129 DEG C first are risen to 0.1 DEG C/min afterwards, then 140 DEG C are risen to 1 DEG C/min,
Finally 280 DEG C are risen to 5 DEG C/min.Mother chrysanthemum polysaccharide monose composition GC chromatograms are shown in Fig. 1.
Foliar fertilizer obtained in embodiment 3 applies composition application method：In ratio of rhizome medicinal material, the seedling stage of industrial crops, by the foliage fertilizer composition
10-2000 times of dilution, in ratio of rhizome medicinal material, the seedling stage of industrial crops, per spraying within 7-10 days 1 time, continuously sprays 2-10 times.
Embodiment 4 applies foliage fertilizer composition of the present invention and improves the yield of corydalis tuber, reduces disease generation
Can improve the yield and downy mildew to corydalis tuber of corydalis tuber, sclerotiniose using the foliage fertilizer composition of embodiment 3 has certain control effect, relatively
Preventive effect reaches 66.1%, and corydalis tuber rate of growth is up to more than 30%.
Panan County Xin Wo towns are experimental field located at, are carried out in May, -2015 in October, 2014.Experiment sets following treatment：(1) fresh water spraying is only used
Corydalis tuber (CK)；(2) conventional pesticide management group；(3) foliage fertilizer composition described in embodiment 3 is diluted 500 times, is sprayed.Totally 3 groups for the treatment of,
Often treatment is weighed 3 times.Random district's groups arrangement.Other cultivation management measures are identical.
The corydalis tuber seed of seed soaking is sowed the artificial weeding during experimental plot, experiment by October, 2014, and other any herbicides are not used,
Using conventional foliar spray method, spraying time is November 15, December 20, December No. 30 totally three times for dispenser.In the corydalis tuber of in May, 2014
Picking time, count corydalis tuber total output, calculate effect of increasing production, the results are shown in Table 2.
The foliage fertilizer composition of the present invention of table 2. is to the growth of field corydalis tuber, the influence of diseases prevention situation
Note：Data are the average value ± standard error of three repeating groups, and the significant difference in P=0.05 levels is represented with lowercase after column data is different,
Capitalization is different to represent that difference is extremely notable in P=0.01 levels.
Embodiment 5 improves sweet potato yield using foliage fertilizer composition of the present invention
Yield experiment is carried out using Zhejiang potato 13, imperial two sweet potatoes of kind of potato No. 9, experiment place is Suichang of Zhejiang Province hair Tian Cun, in 2014
April in year site preparation, base manure of spreading manuer in holes, cuttage.Before June in the same year, the foliage fertilizer composition of embodiment 1 is diluted 800 times, every 7 days foliage-sprays one
Secondary, totally 3 times, harvest time October in the same year counts 2 variety yields, as a result shows, foliage fertilizer composition of the present invention has preferably increasing to sweet potato
Effect is produced, concrete outcome is shown in Table 3.
Influence of the foliage fertilizer composition of the present invention of table 3. to the growth of field sweet potato
Embodiment 6 applies foliage fertilizer composition of the present invention and improves the yield of Radix Angelicae Sinensis, reduces disease generation
In March, 2014 year has carried out Radix Angelicae Sinensis volume increase, disease prevention and control experiment in Gansu Province Dingxi City Minxian County Mei Chuan towns Yong Xing villages：Experiment sets following treatment：
(1) clear water (CK) is only used；(2) foliage fertilizer composition described in embodiment 1 is diluted 1000 times, is sprayed.It is 6m per plot area2, totally 2
Group treatment, often treatment is weighed 3 times, random district's groups arrangement.
Cultivation condition, field management are basically identical, artificial weeding during experiment, and other any herbicides are not used.In the seedling stage (June) of Radix Angelicae Sinensis
Sprayed once every 10 days, totally three times.In the picking time of late October Radix Angelicae Sinensis in 2014, Radix Angelicae Sinensis total output is counted, calculate effect of increasing production, and
The incidence of disease of Radix Angelicae Sinensis is counted, 4 are the results are shown in Table.
Influence of the foliage fertilizer composition of the present invention of table 4. to the yield and the numb stomatosis incidence of disease of field Radix Angelicae Sinensis
Note：Data are the average value ± standard error of three repeating groups, and the significant difference in P=0.05 levels is represented with column data stepmother is different,
The collocation method of foliage fertilizer composition of the embodiment 7 containing mother chrysanthemum polysaccharide
The active component of this foliar fertilizer is mother chrysanthemum polysaccharide, mother chrysanthemum extract and trace element, and wherein mother chrysanthemum polysaccharide, mother chrysanthemum extract is used
Known extracting method, from feverfew mother chrysanthemum (Chrysanthemum indicum) include mother chrysanthemum (Chrysanthemum indicum L.),
The herb of HERBA CHRYSANTHEMI LAVANDULAEFOLII (C.boreale Mak.) or lavandulaleaf dendranthema herb (C.lavandulaefolium (Fisch.) Mak.) etc. includes rhizome and Hua Zhongti
Obtain, add known carrier, auxiliary agent to be configured to pulvis, wettable powder, soluble powder, granule, water dispersible granules, micro- glue
Wafer, tablet.The trace-element fertilizer is the fertilizer that can provide micro elements by plants.Match somebody with somebody this embodiment describes two kinds of conventional aquas
Than, but present disclosure is not limited thereto：Mother chrysanthemum polysaccharide is taken, mother chrysanthemum cauline leaf Aqueous extracts add appropriate water, micro- needed for adding
Secondary element, adds auxiliary agent to be completely dissolved it, so as to prepare the combination of this foliar fertilizer.
Claims (5)
1. the application of foliage fertilizer composition containing mother chrysanthemum polysaccharide in crop yield and disease prevention and control, it is characterised in that：The foliar fertilizer it is main
Active component is trace element, mother chrysanthemum polysaccharide and mother chrysanthemum extract, and remaining composition is solvent, auxiliary agent or carrier；
The foliage fertilizer composition mother chrysanthemum polyoses content therein is the 1%-10% of composition total weight, and mother chrysanthemum extract is composition total weight
0%-50%, the content of element foliar fertilizer is the 5%-50% of composition total weight；
Described foliage fertilizer composition dilutes 10-2000 times of liquid, and in the seedling spraying of crop is completely wet out to blade, interval a couple of days sprays,
Continuously spray 3-10 times.
2. application of the foliage fertilizer composition of mother chrysanthemum polysaccharide as claimed in claim 1 in crop yield and disease control, it is characterised in that the group
Compound trace-element fertilizer includes nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, boron, Tie, Manganese, copper, zinc, molybdenum one kind at least within, and it is in leaf
Mass ratio is 0.002%-25% in leaven composition.
3. application of the foliage fertilizer composition of mother chrysanthemum polysaccharide as claimed in claim 1 in crop yield and disease control, it is characterised in that the combination
Thing prepares the formulation being selected from the group：Wettable powder, soluble powder, effervescent tablet, granule, micro-capsule, suspending agent, missible oil, water and milk.
4. application of the foliage fertilizer composition of mother chrysanthemum polysaccharide as claimed in claim 1 in crop yield and disease control, it is characterised in that described
Mother chrysanthemum polysaccharide be from feverfew mother chrysanthemum (Chrysanthemum indicum) include mother chrysanthemum (Chrysanthemum indicum L.),
The herb of mother chrysanthemum (C.boreale Mak.) or lavandulaleaf dendranthema herb (C.lavandulaefolium (Fisch.) Mak.) etc. includes rhizome and spends middle extraction
Obtain, the copolymer being made up of rhamnose, arabinose, xylose, mannose, glucose, galactolipin, fructose heteroglycan.
5. application of the foliage fertilizer composition of mother chrysanthemum polysaccharide as claimed in claim 1 in crop yield and disease control, it is characterised in that institute
The mother chrysanthemum extract stated be from feverfew mother chrysanthemum (Chrysanthemum indicum) include mother chrysanthemum (Chrysanthemum indicum L.),
The herb of HERBA CHRYSANTHEMI LAVANDULAEFOLII (C.boreale Mak.) or lavandulaleaf dendranthema herb (C.lavandulaefolium (Fisch.) Mak.) etc. includes rhizome and is adopted in spending
Extracted with known method and obtained.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575238A (en) * | 2017-12-20 | 2018-09-28 | 马鞍山市双飞葡萄专业合作社 | A kind of fertilizing method improving grape Vitamin C content |
| CN111387193A (en) * | 2018-12-19 | 2020-07-10 | 浙江农林大学 | Application of antigen in prevention and control of plant diseases caused by sclerotium rolfsii |
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| CN101503323A (en) * | 2008-12-31 | 2009-08-12 | 泰安市农业科学研究院 | Novel pollution-free foliar insecticide-fertilizer and preparation method thereof |
| CN103783091A (en) * | 2012-10-31 | 2014-05-14 | 浙江农林大学 | Application of chrysanthemum indicum polysaccharides agriculturally used as disease-resisting inductors |
| CN104402638A (en) * | 2014-12-22 | 2015-03-11 | 攀枝花市西宇生物科技有限公司 | Method for preparing special leaf fertilizer for mangoes from ageratina adenophora |
| CN104557236A (en) * | 2013-10-16 | 2015-04-29 | 浙江农林大学 | Continuous cropping obstacle resisting agent for largehead atractylodes rhizome |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101503323A (en) * | 2008-12-31 | 2009-08-12 | 泰安市农业科学研究院 | Novel pollution-free foliar insecticide-fertilizer and preparation method thereof |
| CN103783091A (en) * | 2012-10-31 | 2014-05-14 | 浙江农林大学 | Application of chrysanthemum indicum polysaccharides agriculturally used as disease-resisting inductors |
| CN104557236A (en) * | 2013-10-16 | 2015-04-29 | 浙江农林大学 | Continuous cropping obstacle resisting agent for largehead atractylodes rhizome |
| CN104402638A (en) * | 2014-12-22 | 2015-03-11 | 攀枝花市西宇生物科技有限公司 | Method for preparing special leaf fertilizer for mangoes from ageratina adenophora |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108575238A (en) * | 2017-12-20 | 2018-09-28 | 马鞍山市双飞葡萄专业合作社 | A kind of fertilizing method improving grape Vitamin C content |
| CN111387193A (en) * | 2018-12-19 | 2020-07-10 | 浙江农林大学 | Application of antigen in prevention and control of plant diseases caused by sclerotium rolfsii |
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