CN106645680A - Colloidal gold card reader for detecting mycotoxins in livestock farms - Google Patents
Colloidal gold card reader for detecting mycotoxins in livestock farms Download PDFInfo
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- CN106645680A CN106645680A CN201710044695.7A CN201710044695A CN106645680A CN 106645680 A CN106645680 A CN 106645680A CN 201710044695 A CN201710044695 A CN 201710044695A CN 106645680 A CN106645680 A CN 106645680A
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- colloidal gold
- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Urology & Nephrology (AREA)
- Biotechnology (AREA)
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Abstract
The invention particularly discloses a colloidal gold card reader for detecting mycotoxins in livestock farms. The colloidal gold card reader comprises a colloidal gold test paper card reading cavity and a colloidal gold test paper image collecting device, wherein the image collecting device comprises a microprocessor, the microprocessor comprises a communication module, an image sensor, a cold light source and a display system, and the microprocessor is connected and communicated with an intelligent terminal through the communication module; mycotoxin colloidal gold test paper corresponding to built-in programs of the microprocessor is required to be placed in the colloidal gold test paper card reading cavity. According to the colloidal gold card reader, color developing results of the colloidal gold test paper are collected by the image sensor, so that detection result errors caused by errors produced by manual evaluation can be avoided. The detection results of the same batch are processed by one evaluation system and are calculated according to a preset internal standard curve, so that stability of the detection results is facilitated.
Description
Technical field
The present invention relates to colloid gold test paper technical field, in particular it relates to a kind of collaurum of detection plant mould is read
Card instrument.
Background technology
Collaurum is that synthesis is certain by gold chloride (HAuC14) under reducing agent such as white phosphorus, ascorbic acid, tannic acid effect
The gold grain of size, and because electrostatic interaction becomes a kind of stable colloidal state, electronegative hydrophobic sol solution is formed, due to
Electrostatic interaction and become stable colloidal state, therefore claim collaurum.Immuno-gold labeling technology mainly make use of gold grain to have height
The characteristic of electron density, marks at protein combination, under the microscope visible dark brown coloured particles, when these labels are corresponding in gold
When assembling in a large number at part, naked eyes red color visible or pink spot, thus for qualitative or semiquantitative tachysynthesis detection
In method.
Mould is a kind of many cells microorganism, in being widely present in nature, fungi, mould poison is belonged on microbiology
Element is the poisonous and harmful substance produced by mould.In soil, on plant, including cereal, forage grass and ensilage can be sent out
Existing mycotoxin.Hot and humid environmental condition typically favors the breeding of mould and the generation of mycotoxin in feed.Mould
Harm of the toxin to animal is global problem, and China is large agricultural country, but China seed grow industry proportion it is larger be that peasant household dissipates
Support or the cultivation of small-sized plant, scale dispersion, mycotoxin class epidemic prevention and control level and production management ability fall behind, and are occurring
During pestilence, it is difficult to effective control.The traditional experiment detection of mycotoxin there is also program complexity, the technology of detection time length
Problem, testing result out after product sold or shifted, it is impossible to process in time, it is impossible to meet Site Detection needs it is timely,
Efficiently, quickly need.
The content of the invention
For the deficiency that above-mentioned prior art is present, the invention provides a kind of collaurum of detection plant mycotoxin
Chart scanner, to solve above-mentioned technical problem.
The present invention is achieved through the following technical solutions：
A kind of colloidal gold chart reader of detection plant mycotoxin, including colloid gold test paper Card Reader chamber, colloid gold test paper
Image collecting device；Described image harvester also includes microprocessor, and the microprocessor also includes communication module, image
Sensor and cold light source, the microprocessor also includes display system, and the microprocessor is by communication module and intelligent terminal
Connection communication；The colloid gold test paper Card Reader chamber should place mycotoxin collaurum examination corresponding with microprocessor plug-in
Paper.
Preferably, the microprocessor also includes usb interface module, and USB module is connected with microprocessor, has power line concurrently
With the effect of data wire.
Preferably, the display system includes LED display and control panel；The communication module is WIFI module；Institute
Intelligent terminal is stated for smart mobile phone or panel computer.
Relative to prior art, beneficial effects of the present invention：
1st, colloidal gold chart reader of the invention is using the colour developing result that colloid gold test paper is gathered using imageing sensor, can be with
Avoid artificially assessing the error brought, cause testing result error occur.
2nd, the colored intensity of the detection line T line that colloidal gold chart reader of the invention is measured according to chart scanner and the colour developing of C lines
Intensity rate is controlled to the content of mycotoxin in mycotoxin sample to be measured in related quantitative culvert number relation, according to default interior
Calibration curve calculates testing result, and with a batch of testing result same appraisement system is used, and is conducive to the steady of testing result
It is qualitative.
Description of the drawings
Accompanying drawing herein is merged in specification and constitutes the part of this specification, shows the enforcement for meeting the present invention
Example, and be used to explain the principle of the present invention together with specification.
Fig. 1 is the structure block diagram of the present invention.
Specific embodiment
With the following Examples the invention will be further described.
Fig. 1 is the structure block diagram of the present invention, as shown in Figure 1.
A kind of colloidal gold chart reader of detection plant mycotoxin, including colloid gold test paper Card Reader chamber, colloid gold test paper
Image collecting device；Described image harvester also includes microprocessor, and the microprocessor also includes communication module, image
Sensor and cold light source, the microprocessor also includes display system, and the microprocessor is by communication module and intelligent terminal
Connection communication；The colloid gold test paper Card Reader chamber should place mycotoxin collaurum examination corresponding with microprocessor plug-in
Paper.
The quantitative determination result of the colloidal gold chart reader of detection plant mycotoxin is measured according to chart scanner
The content of mycotoxin in the colored intensity of detection line T line and the colored intensity Ratio control of C lines and mycotoxin sample to be measured
In related quantitative culvert number relation, the internal standard directrix curve of instrument is set to, the hand-held Card Reader instrument measures C/T by imaging system
The intensity of reflected light of line, wherein y=ax-m, y represent T/C values, and x represents concentration；N represents slope；M represents displacement.
Preferably, the microprocessor also includes usb interface module, and USB module is connected with microprocessor, has power line concurrently
With the effect of data wire.
Preferably, the display system includes LED display and control panel；The communication module is WIFI module；Institute
Intelligent terminal is stated for smart mobile phone or panel computer.
The colloidal gold chart reader of the present invention can be kept away using the colour developing result that colloid gold test paper is gathered using imageing sensor
Exempt from artificially to assess the error brought, cause testing result error occur；The present invention can be combined present with being connected with intelligent terminal
Work and habits and customs, can allow testing staff easily by Card Reader data storage or it is online send, realize being tested under line,
The Model of Scientific Research of on-line analysis, is conducive to improving operating efficiency.
Antibody and antigen due to being coated with determinand on colloid gold test paper, antibody and antigen are generally all some albumen
Matter, protein is typically all non-refractory, if high temperature irradiation colloid gold test paper, may result in the albumen qualitative change on test paper
Property, the colour developing exception of test paper is affected, cause experimental result inaccurate.So select light source when, prioritizing selection some heating
Measure less light source.
It is highly preferred that described image sensor is CMOS camera or ccd image sensor, the cold light source is 100-
The generic halogen lamp source of 150W or the high pressure xenon lamp light source of 400-600W.
Preferably, mycotoxin colloid gold test paper mark mycotoxin class monoclonal antibody and haemocyanin, can be with
Detection aspergillus flavus B2, aspergillus flavus G1, aspergillus flavus G2, aspergillus flavus F2 toxin and fumonism.
Hereinafter will be described in detail mycotoxin colloid gold test paper composition and preparation process：
The mycotoxin colloid gold test paper includes base plate, sample layer, colloid layer gold, reaction film and adsorptive pads composition, glue
The mycotoxin class monoclonal antibody and animal blood serum albumen of colloid gold label are adsorbed with body gold pad, inspection is coated with reaction film
Antigen and quality control antibody are surveyed, detection antigen is shown as detection line T, is mycotoxin class antigen；Quality control antibody shows
It is sheep anti-mouse igg antibody for nature controlling line C.
Cleaning Principle：The mycotoxin colloid gold test paper of the present invention is based on collaurum double antibodies sandwich technology, detects the nitre of card
It has been coated with mycotoxin antigen (T lines) and sheep anti-mouse igg antibody (C lines) on sour tunica fibrosa in advance.Wherein T lines be detection line, C lines
For nature controlling line.Gold mark antigentic specificity combines mycotoxin antibody, and T lines colour developing depth linearly changes with mycotoxin AC
Become, mycotoxin antibody titer height in sample is measured by colloidal gold chart reader.
The detection line of the present embodiment arranges 1-3 bars, and nature controlling line arranges 1-3 bars.
Preferably, the detection line of the present embodiment arranges 2, and nature controlling line arranges 2.
Gap between detection line is larger, may result in result and difference occurs, in order to be able to preferably control collaurum
The distance between chromatography time difference, two detection lines are 3.5～5.5mm.
The distance of detection line and nature controlling line can also affect the result for chromatographing, spacing too conference growth detection time；Spacing is too
Short, collaurum is excessively assembled can cause detection line and nature controlling line to be capped together, affect subsequent detection instrument to read colour developing result.
Preferably, detection line and Quality Control distance between centers of tracks are controlled in 11-15mm, and preferred distance is 13mm.
Mycotoxin class antigenic quality concentration 0.2-0.6mg/ml in the detection line, optimizes concentration 0.3-0.4mg/ml
The granular size of the collaurum is controlled in the range of 60-100nm, is optimized for 90nm, and colloid gold concentration is
1.35% concentration.
The sample layer of the mycotoxin colloid gold test paper, colloid layer gold, reaction film and adsorptive pads from left to right, from up to
Under combine successively, each layer is overlapped with adjacent layer end.
Compared with prior art, colloid gold test paper of the invention has the advantages that：
1st, the kit detection time in the present invention is fast, convenient, stable and accurate, is adapted to scope wide.
2nd, the present invention not only can realize qualitative detection, can also realize quantitative determination.Experimental result by chart scanner come
Colour developing result is read, the error brought artificially is assessed in reduction.
3rd, detection method of the invention, it is not necessary to the pretreatment of complexity is carried out to sample, operating procedure is brief, it is not necessary to make
Large expensive instrument is used, is not limited by laboratory apparatus condition, be conducive to the scene detected to carry out, be conducive to what Setup Experiments fell behind
Grass-roots unit carries out experiment.
4th, the testing result of colloid gold test paper of the invention than the testing result of actual sample very close to sample detection result
All control 96% or so with the accuracy of legitimate reading；Average deviation CV% of duplicate detection result is controlled within 15%,
Illustrate that the accuracy of the testing result of colloid gold reagent bar is higher, can be suitably used for product quality and meet existing that the market demand reaches
The quantitative colloid gold reagent bar that a large amount of samples screenings in field can be selected.
Experimental example
The colloid gold test paper of the present invention is contrasted with HPLC methods, detects the identical mycotoxin in different samples, collaurum
Detection paper is experimental group, and HPLC methods are control group, and each process is repeated 3 times, and experimental result is averaged, as a result such as the institute of table 1
Show.
The colloid gold test paper of table 1 and HPLC method testing result correction data tables
Detected from the point of view of data and collaurum quantitative reagent bar comparing result after sample according to mass spectrometry, the inspection of collaurum
Testing result of the result than actual sample is surveyed very close to the accuracy of sample detection result and legitimate reading all controls left 96%
It is right；Average deviation CV% of duplicate detection result is controlled within 15%, illustrate colloid gold reagent bar testing result it is accurate
Property it is higher, can be suitably used for product quality and meet the quantitative collaurum that live a large amount of sample screenings can be selected that the market demand reaches
Reagent strip.
Finally it should be noted that above example is only illustrating technical scheme, rather than to present invention guarantor
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (4)
1. a kind of colloidal gold chart reader of detection plant mycotoxin, it is characterised in that including colloid gold test paper Card Reader chamber, glue
The image collecting device of body gold test paper；Described image harvester also includes microprocessor, and the microprocessor also includes communication
Module, imageing sensor and cold light source, the microprocessor also include display system, the microprocessor by communication module with
Intelligent terminal connection communication；The colloid gold test paper Card Reader chamber should place mycotoxin glue corresponding with microprocessor plug-in
Body gold test paper；The mycotoxin colloid gold test paper mark mycotoxin class monoclonal antibody and haemocyanin, can detect Huang
Aspergillus B2, aspergillus flavus G1, aspergillus flavus G2, aspergillus flavus F2 toxin and fumonism.
2. the colloidal gold chart reader of detection plant mycotoxin according to claim 1, it is characterised in that micro- place
Reason device also includes usb interface module, and USB module is connected with microprocessor, has the effect of power line and data wire concurrently.
3. the colloidal gold chart reader of detection plant mycotoxin according to claim 2, it is characterised in that the display
System includes LED display and control panel；The communication module is WIFI module；The intelligent terminal be smart mobile phone or
Panel computer.
4. the colloidal gold chart reader of detection plant mycotoxin according to claim 3, it is characterised in that described image
Sensor is CMOS camera or ccd image sensor, the cold light source for 100-150W generic halogen lamp source or
The high pressure xenon lamp light source of 400-600W.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710044695.7A CN106645680A (en) | 2017-01-21 | 2017-01-21 | Colloidal gold card reader for detecting mycotoxins in livestock farms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710044695.7A CN106645680A (en) | 2017-01-21 | 2017-01-21 | Colloidal gold card reader for detecting mycotoxins in livestock farms |
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| Publication Number | Publication Date |
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| CN106645680A true CN106645680A (en) | 2017-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710044695.7A Pending CN106645680A (en) | 2017-01-21 | 2017-01-21 | Colloidal gold card reader for detecting mycotoxins in livestock farms |
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Cited By (3)
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| CN107402301A (en) * | 2017-08-06 | 2017-11-28 | 潘金文 | A kind of family's poultry pathogens high flux detection chip and its application |
| CN111381030A (en) * | 2020-03-23 | 2020-07-07 | 国家食品安全风险评估中心 | Method and test strip for quantitatively detecting mycotoxin in liquid milk |
| CN111679070A (en) * | 2020-05-12 | 2020-09-18 | 中国计量科学研究院 | Novel rapid, sensitive and accurate quantitative detection method and quantitative detector for coronavirus antibody |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107402301A (en) * | 2017-08-06 | 2017-11-28 | 潘金文 | A kind of family's poultry pathogens high flux detection chip and its application |
| CN111381030A (en) * | 2020-03-23 | 2020-07-07 | 国家食品安全风险评估中心 | Method and test strip for quantitatively detecting mycotoxin in liquid milk |
| CN111381030B (en) * | 2020-03-23 | 2021-02-09 | 国家食品安全风险评估中心 | Method and test strip for quantitatively detecting mycotoxin in liquid milk |
| CN111679070A (en) * | 2020-05-12 | 2020-09-18 | 中国计量科学研究院 | Novel rapid, sensitive and accurate quantitative detection method and quantitative detector for coronavirus antibody |
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Application publication date: 20170510 |