CN101650368A - Method for testing zearalenone toxin by using colloidal gold immunochromatography assay - Google Patents
Method for testing zearalenone toxin by using colloidal gold immunochromatography assay Download PDFInfo
- Publication number
- CN101650368A CN101650368A CN200910307803A CN200910307803A CN101650368A CN 101650368 A CN101650368 A CN 101650368A CN 200910307803 A CN200910307803 A CN 200910307803A CN 200910307803 A CN200910307803 A CN 200910307803A CN 101650368 A CN101650368 A CN 101650368A
- Authority
- CN
- China
- Prior art keywords
- zen
- line
- colloidal gold
- bsa
- monoclonal antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention discloses a method for testing zearalenone toxin by using a colloidal gold immunochromatography assay in the technical field of detection. The method comprises the following steps: respectively coupling ZEN hapten with BSA and OVA by an active ester method to obtain conjugates ZEN-BSA and ZEN-OVA of the ZEN; utilizing the ZEN-BSA as immunogen and utilizing a conventional method to prepare an anti-ZEN monoclonal antibody; using a trisodium citrate reduction method to prepare 40 nm of colloidal gold, and using the colloidal gold to mark the anti-ZEN monoclonal antibody after the dialysis desalination process; spraying the marked monoclonal antibody obtained by the step 3 on a gold marked pad, spraying the ZEN-OVA on a T-line and a rabbit anti-mouse monoclonal antibody on a C-line to assemble a test paper, and then drying the test paper; extracting a sample to be tested by methanol, centrifuging, fetching the supernatant, dropping the supernatant into a sample slot of the test paper to obtain the color developments of the T-line and the C-line, judging and then obtaining the tested result. The method can test ZEN toxin with specificity and has high precision and fast testing speed, and the required time of the method only is 5 to 10 minutes.
Description
Technical field
The present invention relates to the method in a kind of detection technique field, particularly a kind of method that detects the zearalenone toxin with the colloidal gold immunochromatographimethod examination.
Background technology
(Zearalenone is that sickle-like bacteria breeds the secondary metabolite that is produced under certain humidity and temperature conditions ZEN) to the zearalenone toxin.The zearalenone toxin can be present in cereal such as corn, wheat, barley, Chinese sorghum, rye, also can be present in edible containing in the animal tissue of ZEN feed, comprises milk, egg etc.Zearalenone toxin and spontaneous breast cancer, fallopian tubal and uterus oedema, hyperplasia, diseases such as spermatid distortion, apoptosis are relevant.That the zearalenone toxin has is widely distributed, the residence time long, difficult and other toxin have the phenomenon that strengthens toxicity together.After the accession to WTO, the quantum of international trade of agricultural product and relevant food increases day by day, also more and more higher to the requirement of the biological safety of importing and exporting product thereupon, for the smooth listing that guarantees this series products and eater's health, departments such as entry and exit quarantine, customs, manufacturing enterprise, superintendent office press for a kind of fast and convenient zearalenone toxins checking method.
Colloidal gold-labeled method be with collaurum as the tracer label thing, use a kind of immunolabelling technique of antigen-antibody reaction.Collaurum is by gold chloride (HAuCl4) under effects such as reductive agent such as white phosphorus, tannic acid/sodium citrate and trisodium citrate (the present invention use be that trisodium citrate is as reductive agent), aggregate into the gold grain of specific size, because electrostatic interaction becomes a kind of stable colloidal state, so be called collaurum.Colloid gold label comes down to protein high molecular and is adsorbed to the bag on colloid gold particle surface by process.Adsorption mechanism is the negative charge on colloid gold particle surface, forms strong bonded with the positive charge group of protein molecule because of electrostatic interaction.The colloid gold particle of this ball-type has high electron density, can be to multiple boiomacromolecule material such as staphylococcus, immunoglobulin (Ig), toxin, glycoprotein, enzyme, microbiotic, hormone, BSA non-covalent combinations such as (bovine serum albumin(BSA)s), form visible aubergine, make it become immunoreactive good label, therefore be widely used in the detection of various materials.
Enzyme-linked immunosorbent assay since the antigen (or antibody) in the liquid phase need through diffusion could with antigen or the antibody response on the solid phase, need the long period, each step needs washing up hill and dale, and needs specific enzyme and substrate colour developing, therefore length consuming time, program is loaded down with trivial details; High performance liquid chromatography is unfavorable for basic unit's conventional sense use because of its requirement to test sample, instrument and operating personnel.Colloidal gold immunity chromatography can overcome the deficiency of said method, the colloidal gold immune chromatography experiment method is utilized the antigen-antibody reaction principle, the colloid gold label tracer be fixed on antigen on the film or antibody complex formation and be trapped and develop the color, do not need the colour developing of specific enzyme and substrate, do not need the physisorption of the long period between the antigen-antibody yet, and judge the yin and yang attribute result according to whether developing the color, safe and effective, simple and convenient.
The basis of colloidal gold immune chromatography experiment is the immobilization of antigen or antibody and the colloid gold label of antigen or antibody.The antigen or the antibody that are fixed on the NC film still keep its immunologic competence, and the antigen of colloid gold label or antibody had both kept its immunologic competence, and the function of spike is arranged again.When measuring, reacted by capillary action is divided a word with a hyphen at the end of a line forward and gold is marked on the pad antigen or antibody by sample product (measuring wherein antibody or antigen).Continue to divide a word with a hyphen at the end of a line forward, with be fixed on T line (detection line) antigen or antibodies and form immune complex and be trapped and develop the color, be about one and widely be the brownish red band of 1mm, unnecessary golden labeling antibody continues to move forward, close with two resistive connections that are fixed on C line (nature controlling line) and to be trapped and to develop the color, be about one and widely be the brownish red band of 1mm.The amount that this moment, the T line formed examined object matter in gold mark compound and the sample is certain ratio, so can carry out qualitative or semi-quantitative analysis according to the shade that the T line presents.What the present invention adopted is colloidal gold immune chromatography experiment competition combined techniques, be with the fixing T line of the conjugate ZEN-OVA (oralbumin) of ZEN, the monoclonal antibody of the anti-ZEN of colloid gold label is attached to gold mark pad, the polyclonal antibody fixation of C line of the anti-mouse of rabbit, sample liquid is splashed into sample slot, whether colour developing according to the T line comes result of determination, and whether the colour developing of C line judges the quality of test strips own.
Several different methods has been set up in detection about the zearalenone toxin both at home and abroad.The method that detects ZEN at present is mainly high performance liquid chromatography (HPLC), combined gas chromatography mass spectrometry (GC-MS), liquid spectrum and mass spectrometry method (LC-MS).Yet these methods need be carried out strict pre-service to test sample, also need expensive instruments such as high performance liquid chromatograph, require to have the operating personnel of specialty simultaneously, are unfavorable for on-the-spot conventional sense use.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of method of fast detecting zearalenone toxin is provided.But the present invention's specific detection ZEN toxin, the accuracy rate height, detection speed is fast, and required time only is 5～10 minutes.
The present invention is achieved by the following technical solutions, the present invention includes following steps:
Step 3 prepares the collaurum of 40nm with trisodium citrate reduction method, the monoclonal antibody of the anti-ZEN that handles through dialysis desalination with this colloid gold label;
Step 4 sprays to the monoclonal antibody of the mark of step 3 gained on the gold mark pad, and ZEN-OVA is sprayed to the T line, and the monoclonal antibody of the anti-mouse of rabbit sprays to the C line, is assembled into test strips, drying;
In the step 3, the preparation method of described collaurum is specially: with the 0.005%HAuCl of 100ml
4Solution is heated to boiling, adds 1% trisodium citrate aqueous solution of 0.5～1ml afterwards, boils 7～10min, adds tri-distilled water at last to 100ml, prepares the colloidal gold solution of 40nm; Wherein, percentage is the weight percent by volume.
In the step 3, described mark is specially: under stirring condition, add monoclonal antibody in colloidal gold solution, make its final concentration reach 40ug/mL, hatch 5min under 25 ℃, use 0.1mol/L K
2CO
3The pH that regulates colloidal gold solution is 9.0, the final concentration that adds 10%BSA to BSA then is 0.1%, the centrifugal 20min of 10000rpm, abandon supernatant, be that 9.0 Tris damping fluid recovers with 0.01mM pH again, repeat 1 time that 1/10 the 0.01mM pH that afterwards precipitation is resuspended in original volume is in 9.0 the Tris damping fluid, the final concentration that adds 10%BSA to BSA at last is 0.1%, 4 ℃ and stores for future use; Wherein, percentage is the weight percent by volume.
In the step 4, described drying is 37 ℃ of oven dryings.
In the step 5, described methyl alcohol is that volume fraction is 80% methyl alcohol.
In the step 5, described centrifugal be centrifugal 15 minutes of 2500g.
In the step 5, described evaluation is specially: the brownish red band occurs on the C line, contain the zearalenone toxin in the testing sample; On T line and C line, the brownish red band all occurs, do not contain the zearalenone toxin in the testing sample.
The present invention is in view of testing sample if the ZEN toxin is arranged, because capillary effect chromatography forward moves, ZEN in the sample liquid marks the compound that monoclonal antibody forms with gold, competed the chance that antigen combines with gold mark monoclonal antibody on the T line, so collaurum can not or only can be dammed by the antigen on the T line and deposit on a small quantity, very shallowly judge that ZEN is arranged in the sample so do not develop the color or develop the color with the T line; Correspondingly testing sample is not if there is ZEN, and the T line then develops the color, and presents red stripes clearly, judges the content of ZEN in the test sample such as food, animal product thus.
Compared with prior art, the present invention has following beneficial effect: detected object of the present invention is single and with strong points, the accuracy rate height.Detection speed is fast, required time is short, only need 5～10 minutes, do not need just can use the inventive method to detect through the professional of training, satisfy grain store inspection departments such as marketing organization, entry and exit, customs fast, the requirement of the ZEN content of toxins that judges rightly, and be convenient to that basic unit promotes and utilization.
Description of drawings
Fig. 1 is the structural drawing of embodiment of the invention test strips;
Fig. 2 is the figure as a result of embodiment of the invention test strips;
Wherein, the positive result schematic diagram of a, the negative result schematic diagram of b.
Embodiment
Below embodiments of the invention are elaborated: present embodiment has provided detailed embodiment and process being to implement under the prerequisite with the technical solution of the present invention, but protection scope of the present invention is not limited to following embodiment.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, for example the Sambrook equimolecular is cloned: laboratory manual (NewYork:Cold Spring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.
This example at first is connected to ZEN OVA or BSA and upward obtains to have immunogenic comlete antigen, and by dialysis and this antigen of ultrafiltration centrifugal purification; The antigen ZEN-BSA of this BSA and ZEN coupling is prepared monoclonal antibody as immunogen immune Balb/C mouse, and adopt the indirect enzyme-linked immunosorbent adsorption experiment to measure anti-ZEN antibody titer as envelope antigen with the antigen ZEN-OVA of OVA and ZEN coupling; Monoclonal antibody purification; Prepare the collaurum of 40nm and the monoclonal antibody protein of mark purifying with the trisodium citrate reduction method of 1% (W/V); Down payment mark monoclonal antibody, ZEN-OVA coupled antigen, the anti-mouse polyclonal antibody spraying of rabbit gold mark pad, detection line (T line), nature controlling line (C line) are assembled into test strips then, put 37 ℃ of oven for drying, deposit-20 ℃ of refrigerators and preserve standby; During detection, treat that test strips returns to room temperature in packing after, test lead is inserted in the sample liquid 5～10 minutes observationss.
Embodiment
(1) preparation of immunizing antigen: get 0.33ml (3mg/ml) ZEN, be mixed in the 1.2ml pyridine, add 2mg O-ethyloic azanol, stirring at room reaction 24h.After the vacuum drying, add 4ml distilled water, and make its dissolving, adjust pH to 8.0.Unreacted ZEN removes (3ml benzene, extracting is 3 times altogether) with the benzene extracting, removes the benzene phase, keeps water.Water is transferred pH to 3.0, with ethyl acetate extracting (10ml ethyl acetate, extracting is 4 times altogether), extracts the ester phase out, abandons water.Ester dries up after filtering with anhydrous sodium sulfate.Crystal after drying up is dissolved in the dioxane that the 0.5ml alkali alumina handled.Take by weighing 20mgBSA and be dissolved among the PBS of 0.7ml 0.05mol/L (pH7.2), two solution are slowly mixed in 4 ℃.1mg NHS and 2mg DCC are dissolved in the 0.2ml dioxane, and slowly drip this solution.Mixed solution is placed stirring at room reaction 16h.Transfer pH to 6.0, dry up in the fuming cupboard, slowly drip DCC solution (2mgDCC is dissolved in the 0.2ml dioxane).Stirring at room reaction 48h.At last with PBS dialysis 2～3d ,-20 ℃ of preservations.
(2) preparation of envelope antigen: the preparation of similar immunizing antigen is changed to OVA with BSA and gets final product.
The ZEN-BSA comlete antigen is dissolved among the PBS, measures the concentration of carrier protein in the comlete antigen.The Freund's complete adjuvant of antigen and equivalent is fully emulsified, hypodermic injection immunity Balb/C mouse in 6 age in week, every 0.1ml; Two exempt from: after two weeks, use incomplete Freund instead, use the same method and dosage, carry out immunity; Three exempt from, and operation is exempted from two.Immunity after 5 days eyeground vein blood sampling survey and tire, booster immunization when above that reaches 1: 10000 of tiring: the antigen 0.1ml that lumbar injection does not add adjuvant, put to death mouse after three days, get its spleen, merge with the myeloma cell.Screen positive hybridoma cell with indirect ELISA method.Prepare mouse ascites in a large number by mouse peritoneal injection hybridoma, behind the filtration of ascites process, the centrifugal preliminary purification, adopt sad method and affinity chromatography purifying ascites.
Step 3, the preparation of collaurum and the mark of monoclonal antibody
0.005% (W/V) HAuCl4 solution with 100ml is heated to boiling earlier, 1% (W/V) trisodium citrate aqueous solution that adds 0.5～1ml rapidly, begin some blueness, light blue then, blue, heating occurs red again, boil 7～10min and transparent claret occurs, add tri-distilled water at last, prepared the colloidal gold solution of 40nm like this to 100ml.Use the Electronic Speculum microscopy then, guarantee that the gold grain for preparing makes its size consistent as far as possible, evenly, particle diameter is about 40nm, otherwise preparation again.
Antibody protein to be marked uses the collaurum of the 40nm for preparing to come mark polyclonal antibody albumen with 48 hours desalinations of sodium chloride solution dialysis of 0.005mol/L then.Concrete steps are: 1. add antibody protein in the colloidal gold solution in stirring rapidly and make its final concentration reach 40ug/mL, hatch 5min under 25 ℃ of room temperatures; 2. the pH with 0.1mol/L K2CO3 adjusting gold solution is 9.0, adds 10% (W/V) BSA then and comes the stable colloid gold solution to final concentration 0.1%, reaction 5min; 3. the centrifugal 20min of 10000rpm abandons supernatant, uses the damping fluid of 0.01mM Tris (pH=9.0) to recover again, repeats 1 time, to remove the antibody that does not combine with gold grain; 4. for the last time precipitation is resuspended in 1/10 0.01mM Tris (pH=9.0) damping fluid of original volume after centrifugal, adding BSA at last is 0.1% (W/V) to final concentration, and NaN3 is to final concentration 0.02% (W/V), and 4 ℃ store for future use.More than it should be noted that in the operation should impure particulate, available high speed centrifugation or miillpore filter pre-service in all solution.
Step 4, the assembling of colloidal gold strip
The monoclonal antibody protein that mark is good sprays to gold and marks on the pad, and spraying ZEN-OVA coupled antigen is to the T line, and the spraying two anti-C lines that arrive assemble test strips then, and 37 ℃ of oven dryings are put 4 ℃ of refrigerators and preserved standby.The assembling sequence of test strips such as accompanying drawing 1, the assembling sequence of test strips such as accompanying drawing 1, order from down to up is 1 to be adsorptive pads for sample pad, 5 for gold mark pad, 4 for nitrocellulose filter, 3 for plastics end liner, 2.
Sample to be checked is splashed in the sample slot of test strips, because capillary effect, the chromatography direction of liquid upwards, if contain ZEN in the liquid to be checked, when liquid to be measured enters test lead, because capillary effect moves forward, ZEN marks monoclonal antibody (Au-Ab) with the gold on the gold mark pad and forms Au-Ab-ZEN bigeminy compound, compound continues the chromatography swimming on the NC film, can't combine with the ZEN-OVA coupled antigen on the detection line (T line) and can not be fixed on T line antigen and be retained down, can't form visible brownish red band; The Au-Ab-ZEN compound continues migration forward because the chromatography effect, combines and is retained down with the anti-mouse polyclonal antibody of rabbit on being fixed on nature controlling line (C line), forms visible brownish red band, qualitatively judges; Probably judge detected ZEN content according to detection line colour developing depth degree, belong to sxemiquantitative, and then carry out quantitatively, shown in a among Fig. 2 in conjunction with enzyme linked immune assay.
If liquid to be measured does not contain ZEN, the gold mark mouse monoclonal antibody of gold mark pad continues to move forward, then the ZEN-OVA with detection line (T line) combines, form Au-Ab-ZEN-OVA and be trapped, form visible brownish red band, unnecessary gold mark monoclonal body continues chromatography upwards, and is fixed on two resistive connections on the nature controlling line (C line) and closes and form the bigeminy compound and be retained down, form visible brownish red band, shown in b among Fig. 2.
The method of present embodiment is the ZEN in the test sample directly, does not need professional training, and is easy to operate, quick, can obtain the result in 5～10 minutes, can detect the purpose of ZEN quick, easy, in time.
Claims (7)
1, a kind of method with colloidal gold immunochromatographimethod examination detection zearalenone toxin is characterized in that, comprises the steps:
Step 1, with the ZEN haptens by active ester method respectively with BSA and OVA coupling, obtain conjugate ZEN-BSA and the ZEN-OVA of ZEN;
Step 2 utilizes ZEN-BSA as immunogene, adopts conventional method to prepare the monoclonal antibody of anti-ZEN;
Step 3 prepares the collaurum of 40nm with trisodium citrate reduction method, the monoclonal antibody of the anti-ZEN that handles through dialysis desalination with this colloid gold label;
Step 4 sprays to the monoclonal antibody of the mark of step 3 gained on the gold mark pad, and ZEN-OVA is sprayed to the T line, and the monoclonal antibody of the anti-mouse of rabbit sprays to the C line, is assembled into test strips, drying;
Step 5, the testing sample methanol extraction is centrifugal, get supernatant, supernatant is splashed in the sample slot of test strips, obtain the colour developing of T line and C line, identify, obtain the result.
2, the method that detects the zearalenone toxin with the colloidal gold immunochromatographimethod examination according to claim 1, it is characterized in that, in the step 3, the preparation method of described collaurum is specially: the 0.005%HAuCl4 solution of 100ml is heated to boiling, 1% trisodium citrate aqueous solution that adds 0.5～1ml afterwards, boil 7～10min, add tri-distilled water at last, prepare the colloidal gold solution of 40nm to 100ml; Wherein, percentage is the weight percent by volume.
3, the method that detects the zearalenone toxin with the colloidal gold immunochromatographimethod examination according to claim 1, it is characterized in that, in the step 3, described mark is specially: under stirring condition, in colloidal gold solution, add monoclonal antibody, make its final concentration reach 40ug/mL, hatch 5min under 25 ℃, the pH that regulates colloidal gold solution with 0.1mol/L K2CO3 is 9.0, the final concentration that adds 10%BSA to BSA then is 0.1%, the centrifugal 20min of 10000rpm abandons supernatant, is 9.0 Tris damping fluid recovery again with 0.01mM pH, repeat 1 time, 1/10 the 0.01mM pH that afterwards precipitation is resuspended in original volume is that the final concentration that adds 10%BSA to BSA at last is 0.1%, 4 ℃ and stores for future use in 9.0 the Tris damping fluid; Wherein, percentage is the weight percent by volume.
4, the method with colloidal gold immunochromatographimethod examination detection zearalenone toxin according to claim 1 is characterized in that in the step 4, described drying is 37 ℃ of oven dryings.
5, the method with colloidal gold immunochromatographimethod examination detection zearalenone toxin according to claim 1 is characterized in that in the step 5, described methyl alcohol is that volume fraction is 80% methyl alcohol.
6, according to claim 1ly detect the method for zearalenone toxin, it is characterized in that with colloidal gold immunochromatographimethod examination, in the step 5, described centrifugal be centrifugal 15 minutes of 2500g.
7, the method with colloidal gold immunochromatographimethod examination detection zearalenone toxin according to claim 1 is characterized in that in the step 5, described evaluation is specially: the brownish red band occurs on the C line, illustrate to contain the zearalenone toxin in the testing sample; On T line and C line, the brownish red band all occurs, illustrate not contain the zearalenone toxin in the testing sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910307803A CN101650368A (en) | 2009-09-27 | 2009-09-27 | Method for testing zearalenone toxin by using colloidal gold immunochromatography assay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910307803A CN101650368A (en) | 2009-09-27 | 2009-09-27 | Method for testing zearalenone toxin by using colloidal gold immunochromatography assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101650368A true CN101650368A (en) | 2010-02-17 |
Family
ID=41672648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910307803A Pending CN101650368A (en) | 2009-09-27 | 2009-09-27 | Method for testing zearalenone toxin by using colloidal gold immunochromatography assay |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101650368A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010838A (en) * | 2010-08-27 | 2011-04-13 | 江苏省农业科学院 | Bacterial strain for degrading zearalenone toxin and application thereof |
| CN102279268A (en) * | 2011-07-29 | 2011-12-14 | 上海交通大学 | Method for simultaneously detecting zearalenone and Fumonisins |
| CN102295697A (en) * | 2010-06-25 | 2011-12-28 | 中国医学科学院药用植物研究所 | Conjugate of cationized carrier protein and zearalenone constructed by one-step method |
| CN102313810A (en) * | 2011-07-29 | 2012-01-11 | 上海交通大学 | Chemiluminescent immunoassay method for rapidly detecting zearalenone toxin |
| CN102520178A (en) * | 2011-12-07 | 2012-06-27 | 上海交通大学 | Method for simultaneous quantitative detection of zearalenone and fumonisin B1 |
| CN102520177A (en) * | 2011-12-07 | 2012-06-27 | 上海交通大学 | Method for quantitative detection of zearalenone |
| CN107271431A (en) * | 2017-05-25 | 2017-10-20 | 中国农业科学院农产品加工研究所 | A kind of method of zearalenone toxin in quick detection vegetable oil |
| CN112698027A (en) * | 2020-12-14 | 2021-04-23 | 广东唯实生物技术有限公司 | Hapten immunochromatography detection reagent |
-
2009
- 2009-09-27 CN CN200910307803A patent/CN101650368A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295697A (en) * | 2010-06-25 | 2011-12-28 | 中国医学科学院药用植物研究所 | Conjugate of cationized carrier protein and zearalenone constructed by one-step method |
| CN102010838A (en) * | 2010-08-27 | 2011-04-13 | 江苏省农业科学院 | Bacterial strain for degrading zearalenone toxin and application thereof |
| CN102010838B (en) * | 2010-08-27 | 2012-02-01 | 江苏省农业科学院 | Bacterial strain for degrading zearalenone toxin and application thereof |
| CN102279268A (en) * | 2011-07-29 | 2011-12-14 | 上海交通大学 | Method for simultaneously detecting zearalenone and Fumonisins |
| CN102313810A (en) * | 2011-07-29 | 2012-01-11 | 上海交通大学 | Chemiluminescent immunoassay method for rapidly detecting zearalenone toxin |
| CN102520178A (en) * | 2011-12-07 | 2012-06-27 | 上海交通大学 | Method for simultaneous quantitative detection of zearalenone and fumonisin B1 |
| CN102520177A (en) * | 2011-12-07 | 2012-06-27 | 上海交通大学 | Method for quantitative detection of zearalenone |
| CN107271431A (en) * | 2017-05-25 | 2017-10-20 | 中国农业科学院农产品加工研究所 | A kind of method of zearalenone toxin in quick detection vegetable oil |
| CN112698027A (en) * | 2020-12-14 | 2021-04-23 | 广东唯实生物技术有限公司 | Hapten immunochromatography detection reagent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101650368A (en) | Method for testing zearalenone toxin by using colloidal gold immunochromatography assay | |
| CN101988924B (en) | Test strip for detecting anti-cyclic citrullinated peptide antibody in blood and preparation method | |
| CN101661043A (en) | Method for detecting fumonisin by colloidal gold immunochromatographic test | |
| CN101413955B (en) | ELISA test box for detecting zearalenone and preparing and detecting method thereof | |
| CN106918704A (en) | Synchronous detection aflatoxin and time-resolved fluoroimmunoassay chromatography kit, the preparation method and application of carbaryl composite pollution | |
| CN102279268A (en) | Method for simultaneously detecting zearalenone and Fumonisins | |
| CN101158683A (en) | Colloidal gold chromatography semi-quantitative determination 2,4-D test paper and preparation method thereof | |
| CN102087284A (en) | Preparation method of reagent board for rapidly detecting aflatoxin B1 | |
| CN111187346B (en) | Colloidal gold test strip for detecting fipronil and metabolites thereof and preparation method thereof | |
| CN106084059A (en) | The general specific antibody of anti-Capsaicinoids, test strips and kitchen waste grease immunochromatography method for quick identification | |
| CN105158461A (en) | Rapid detecting method and corresponding test strip for acetamiprid residues in tea | |
| CN103336132A (en) | Detection method of lactoferrin in tears and dedicated colloidal gold detecting card thereof | |
| CN105067599A (en) | Chemiluminescence immune detection kit for detecting pro-gastrin-releasing peptide | |
| CN108776219A (en) | A kind of immuno-chromatographic test paper strip of the thin Alternaria alternata ketone acid of quick detection | |
| CN101477124A (en) | Method for detecting melamine and its special test paper | |
| CN105929154A (en) | Test strip and kit for rapid detection of antibody against brucellosis | |
| CN101434655B (en) | Preparation of egg yolk antibody against citrinin | |
| CN102879574A (en) | Bar/pat gene transfer plant and rapid test strip for derivative product of bar/pat transgenic plant | |
| CN202033368U (en) | Reagent plate for detecting aflatoxins M1 in dairy | |
| CN102520179A (en) | Quantitative detection method of fumonisins B1 | |
| CN107271665A (en) | A kind of test strips for detecting salbutamol and its application | |
| CN107589265A (en) | A kind of time-resolved fluorescence test strips for detecting aflatoxin B1 and its application | |
| CN101424688A (en) | Method for detecting chlorpromazine by colloidal gold immune chromatography test | |
| CN105586316B (en) | A kind of hybridoma cell strain that secreting anti-quinolone drugs monoclonal antibody and its application | |
| CN102520177A (en) | Method for quantitative detection of zearalenone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100217 |