CN106645367A - Online detector combining ultrasonic atomization and ion mobility spectrometry and application - Google Patents
Online detector combining ultrasonic atomization and ion mobility spectrometry and application Download PDFInfo
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- CN106645367A CN106645367A CN201510736073.1A CN201510736073A CN106645367A CN 106645367 A CN106645367 A CN 106645367A CN 201510736073 A CN201510736073 A CN 201510736073A CN 106645367 A CN106645367 A CN 106645367A
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- ultrasonic atomizatio
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/622—Ion mobility spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention designs an online detector combining ultrasonic atomization and ion mobility spectrometry. According to the detector, an ion mobility spectrometry technology is adopted as a basic detection technology and is combined with an ultrasonic atomization technology for samples. A blood sample is collected to the surface of a sampling piece without pretreatment, and then, the sample is turned into a fine mist drop state with the ultrasonic atomization technology and carried into the detector by the aid of carrier gas for online detection analysis. The online detector is applied to online detection of propofol in human blood in an operation and can guide a doctor in clinical administration.
Description
Technical field
The present invention is based on Ion mobility spectrometry, with reference to sample ultrasonic atomization and headspace sampling technology, if
A kind of on-line detector is counted.Detection means and method in the present invention is used to into Propofol in blood to face
Bed administration depth analysis, instruct the accurate medication of clinical anesthesia doctor.
Background technology
Using electronics high frequency oscillation, (frequency of oscillation is 1.7MHz or 2.4MHz to ultrasonic atomizer, is exceeded
The audibility range of people, the electronic is to human body and animal fanout free region), by the high frequency of ceramic atomizing piece
Resonance, aqueous water minor structure is broken up and the elegant water smoke of nature is produced, and is not required to heat or add appoint
What chemical reagent.Compare with heating atomization mode, energy saving 90%.
Propofol is a kind of conventional Intravenous Anesthesia agent, and anesthesia induction, anesthesia dimension have been widely used in
Hold and ICU for heavy patient calmness.In surgical procedure, the concentration of propofol in patient blood is often
There is close correlation with its depth of anesthesia, therefore the concentration of Propofol in patient blood is monitored in art
Have great importance for Anesthesia Monitoring.
Being presently used for determining the method for Propofol in blood mainly includes that high performance liquid chromatography and makings join
With etc..However, biological sample of the blood as a complexity, necessary before it carries out chromatographic isolation
Sample pretreatment is carried out, so as to cause above method time-consuming longer, and mostly off-line analysis, it is impossible to it is complete
The demand of full up foot clinically Anesthesia Monitoring.
Ion mobility spectrometry (Ion Mobility Spectrometry, IMS) technology is 20th century 70
A kind of quick separating detection technique that age occurs, compared with traditional mass spectrum, chromatographic apparatus, has
Simple structure, sensitivity is high, and analyze speed is fast, the characteristics of reliable results.The IMS that at present we study
Be widely used in chemical warfare agent, drugs, explosive detection, environmental monitoring, monitoring poisonous gas,
The fields such as fire monitoring, water pollution monitoring and food inspection.Ionic migration spectrometer mainly by ion gun,
Ion gate, migration area and detector composition.Ion gun makes sample molecule, N2、O2Ionize with vapor,
The ion of generation is easy to that ion molecule reaction occurs with molecule, obtains multi-products ion.Ion exists
The ion gate opened by periodicity under the ordering about of electric field enters migration area, the neutral drift gas point with adverse current
Son is continually colliding, because these ions have in the electric field different migration rates so that different
Ion is separated, and successively reaches detector.The present invention is based on Ion mobility spectrometry, with reference to sample
Ultrasonic atomizatio and headspace sampling technology, devise a kind of on-line detector.
The content of the invention
A kind of on-line detector of ultrasonic atomizatio in combination with ion mobility spectrometry, including sampling device and from
Sub- mobility spectrometer；Sampling device includes ultrasonic atomizatio room, ultrasonic atomizer and takes blood sampling sheet and ultrasound
Atomizing piece；
Ultrasonic atomizatio room be a closed container, its upper end be provided with one as fluid sample entrance through hole,
Fluid sample entrance is provided with dismountable closure；Ultrasonic atomizatio room is respectively arranged on the left side and the right side a through hole,
One is carrier gas inlet, another for carrier gas gas outlet, and ultrasonic atomizatio piece is placed with ultrasonic atomizatio room,
Ultrasonic atomizatio piece top is placed with sampling sheet, and the ultrasonic atomizatio outdoor below ultrasonic atomizatio piece is provided with
Ultrasonic atomizer, i.e. ultrasonic atomizer are arranged at the lower section of ultrasonic atomizatio room；Fluid sample entrance is located at
Sampling sheet top；
Carrier gas inlet is connected with carrier gas source of the gas, the carrier gas air inlet phase of carrier gas outlet and transference tube
Connection.
Bottom in ultrasonic atomizatio room is provided with a groove, has ultrasonic atomizatio in the bottom surface tiling of groove
Piece；Sampling sheet is placed in ultrasonic atomizatio piece top, and sampling sheet is mutually amplexiformed with ultrasonic atomizatio piece.
Ultrasonic atomizatio room bottom is by ultrasonic atomizatio room sealed bottom by a dismountable dividing plate；Every
Plate upper surface is provided with a groove, and the surface tiling ultrasonic atomizatio piece in groove, sampling sheet is placed in ultrasound
Atomizing piece top；
3-8 root springs are uniformly provided with dividing plate lower section, spring lower section sets support, spring and the side of support one
Ultrasonic atomizer is fixed in face, on the other hand by the flexible realizing opening and closing ultrasonic mist of spring
Change the dividing plate of room bottom.
Fluid sample entrance is provided with dismountable closure, and when sample is added dropwise closure is opened, and this leads to
Open in hole；Closure is closed when ultrasonic atomizatio and headspace sampling process, this through hole is closed；
Sample is done after the pre-treatment of ultrasonic atomizatio head space again using the principle of ultrasonic atomizatio for sampling device
Sample introduction is analyzed.
Sampling sheet is the polytetrafluoroethylene (PTFE) high temperature samples cloth or glass consistent with ultrasonic atomizatio piece size shape
Fibrous paper；Designed size is generally the wafer architecture of diameter 1-3cm.
When ultrasonic atomizer works, ultrasonic atomizatio piece is driven during sampling sheet concussion, ultrasonic atomizatio room
Bottom baffles are set on state, it is to avoid the interference that ultrasonic vibration brings, after concussion process terminates,
Ultrasonic atomizatio room bottom baffles are set to off closed state, carry out headspace sampling analysis.
On-line detector in the present invention is used in blood in the detection process of propofol agent.Blood
The ultrasonic atomizatio time control of liquid sample is in 1-10S.
Transference tube includes ionized region, ion gate, migration area, the grid sequentially coaxially arranged in housing
Net and ion receiving pole；Drift gas entrance is provided near the housing sidewall of ion receiving pole, gas entrance is floated
It is connected with drift gas source of the gas；Housing sidewall between ion gate and ionization source is provided with gas vent, should
Gas vent is vented.
The present invention is based on Ion mobility spectrometry, with reference to ultrasonic atomizatio and headspace sampling technology, devises
A kind of on-line detector, is used for the detection means and method in the present invention Propofol clinic in blood and gives
Medicine depth analysis, improve the detection sensitivity of Propofol in blood.
Advantages of the present invention is as follows：
1. compared with traditional gas chromatography, using the detector in the present invention as analysis sample
Means there is advantages below：Fluid sample can be without the need for the direct ultrasound in injector of complicated pre-treatment
Atomization, it is possible to achieve fluid sample on-line checking；
2. sampling device is simple, and analysis detection speed is fast, and the single sample testing time is less than 1min；
The operating cost of instrument is low, and running stores are few；
3. the sampling device that detector is combined in the present invention saves energy without the need for electrical heating Thermal desorption technology
Source.
Description of the drawings
Fig. 1 is the on-line detector apparatus structure principle schematic in the present invention；1 be microsyringe,
2 is silica gel tightening nut (containing " O " circle), 3 is ultrasonic atomizatio room, 4 is to take blood sampling sheet and ultrasound
Atomizing piece, 5 be ultrasonic atomizer, 6 be carrier gas inlet, 7 be carrier gas outlet, 8-15 be migration tube knot
Structure；8 be sample gas enter migration tube inlet, 9 be migration tube ionized region, 10 be ion gate, 11 be
Migration tube migration area, 12 be aperture plate, 13 be ion receiving pole, 14 for drift gas entrance, 15 be outlet
Mouthful.
Specific embodiment
Using traditional uniform field migration spectrum, its structural representation is shown in Fig. 1 to Propofol in blood.This instrument
Mainly include following components：1 is microsyringe, 2 is silica gel tightening nut (containing " O " circle),
3 be ultrasonic atomizatio room, 4 for take blood sampling sheet and ultrasonic atomizatio piece, 5 be ultrasonic atomizer, 6 to carry
Gas entrance, 7 are that carrier gas is exported, 8-15 is migration tube structure；8 be sample gas enter migration tube inlet,
9 be migration tube ionized region, 10 be ion gate, 11 be migration tube migration area, 12 be aperture plate, 13 be from
Sub- receiving pole, 14 are gas outlet for drift gas entrance, 15.
Cavity lower surface is amplexiformed with the structure of ultrasonic atomizer outer chamber upper surface two outside ultrasonic atomizatio room,
It is internally formed the cavity of a sky；There is silica gel tightening nut at ultrasonic atomizatio room upper surface center (containing " O "
Circle) can open, sample is instilled by microsyringe.Bottom in ultrasonic atomizatio room be provided with one it is recessed
Groove, has ultrasonic atomizatio piece in the bottom surface tiling of groove；Sampling sheet is placed in ultrasonic atomizatio piece top, and adopts
Print is mutually amplexiformed with ultrasonic atomizatio piece.Ultrasonic atomizer bottom below ultrasonic atomizatio room is by one
Dismountable dividing plate is by ultrasonic atomizatio room sealed bottom.3-8 root springs are uniformly provided with dividing plate lower section,
Spring lower section sets support, and on the one hand spring and support are fixed ultrasonic atomizer, on the other hand by bullet
The flexible dividing plate to realize opening and closing ultrasonic atomizatio room bottom of spring.
Using operating procedure to open the upper end tightening nut 2 of ultrasonic atomizatio room 3, taken with microsyringe 1
Blood sample Jing ultrasonic atomizatios room upper end through hole is added dropwise on the sampling sheet 4 of ultrasonic atomizatio room bottom；So
Afterwards ultrasonic atomizer stretches dividing plate by support, and control dividing plate does not contact ultrasonic atomizatio chamber room lower limb,
Then ultrasonic atomizatio piece 1-10S, then moment closing ultrasonic atomizatio room are started.Carrier gas Jing carrier gas inlets 6 are taken
With the headspace sample gas after ultrasonic atomizatio transference tube air inlet 8 is entered by transference tube by 7
(8-15) it is analyzed detection.
After completing above-mentioned once analysis detection, ultrasonic atomizer appliance stand spring is compressed, more renewed
The pending analysis detection next time of sampling sheet.
Carrier gas and drift gas source of the gas adopt the source of the gas (air) of purified treatment, and carrier gas in test
400sccm, floats gas 600sccm, and source of the gas humidity is 0-20ppm.
On-line detector by ultrasonic atomizatio in combination with ion mobility spectrometry is used for according to above-mentioned implementation process
The detection of propofol agent in blood.
Propofol injection drug standards are taken, concentration is 10000ng/ μ L；It is dilute step by step with blank plasma
Release, be configured to blood concentration be 1,2.5,5,7.5, the sample of 10ng/ μ L, it is standby.Single mark
The testing result of quasi- sample, when reagent ion peak transit time is in 6ms, the detection of Propofol standard is moved
Shift time is in 9.5ms.In blood concentration 1-10ng/ μ L concentration ranges, testing standard curvilinear equation
And calculate detection sensitivity.The on-line detector adopted in the present invention can improve detection sensitivity
5-10 times.
This apparatus is used for into the detection of propofol agent clinic blood concentration, Clinical practice is met
Demand.A set of new apparatus and method are provided for the accurate medication of Anesthetist.
Claims (8)
1. on-line detector of a kind of ultrasonic atomizatio in combination with ion mobility spectrometry, it is characterised in that：Bag
Include sampling device and ionic migration spectrometer；Sampling device includes ultrasonic atomizatio room, ultrasonic atomizer and adopts
Print；
Ultrasonic atomizatio room be a closed container, its upper end be provided with one as fluid sample entrance through hole,
Fluid sample entrance is provided with dismountable closure；Ultrasonic atomizatio room is respectively arranged on the left side and the right side a through hole,
One is carrier gas inlet, another for carrier gas gas outlet, and ultrasonic atomizatio piece is placed with ultrasonic atomizatio room,
Ultrasonic atomizatio piece top is placed with sampling sheet, and the ultrasonic atomizatio outdoor below ultrasonic atomizatio piece is provided with
Ultrasonic atomizer, i.e. ultrasonic atomizer are arranged at the lower section of ultrasonic atomizatio room；Fluid sample entrance is located at
Sampling sheet top；
Carrier gas inlet is connected with carrier gas source of the gas, the carrier gas air inlet phase of carrier gas outlet and transference tube
Connection.
2. on-line detector according to claim 1, it is characterised in that：In ultrasonic atomizatio room
Bottom be provided with a groove, in groove bottom surface tiling have ultrasonic atomizatio piece；Sampling sheet is placed in ultrasound
Above atomizing piece, and sampling sheet is mutually amplexiformed with ultrasonic atomizatio piece.
3. on-line detector according to claim 1 and 2, it is characterised in that：Ultrasonic atomizatio room
Bottom is by ultrasonic atomizatio room sealed bottom by a dismountable dividing plate；Dividing plate upper surface is provided with one
Individual groove, the surface tiling ultrasonic atomizatio piece in groove, sampling sheet is placed in ultrasonic atomizatio piece top；
3-8 root springs are uniformly provided with dividing plate lower section, spring lower section sets support, spring and the side of support one
Ultrasonic atomizer is fixed in face, on the other hand by the flexible realizing opening and closing ultrasonic mist of spring
Change the dividing plate of room bottom.
4. on-line detector according to claim 1, it is characterised in that：
Fluid sample entrance is provided with dismountable closure, and when sample is added dropwise closure is opened, and this leads to
Open in hole；Closure is closed when ultrasonic atomizatio and headspace sampling process, this through hole is closed；
Sample is done after the pre-treatment of ultrasonic atomizatio head space again using the principle of ultrasonic atomizatio for sampling device
Sample introduction is analyzed.
5. on-line detector according to claim 1, it is characterised in that：Sampling sheet is and ultrasound
The consistent polytetrafluoroethylene (PTFE) high temperature samples cloth of atomizing piece size shape or all-glass paper；Designed size one
As for diameter 1-3cm wafer architecture.
6. on-line detector according to claim 3, it is characterised in that：Ultrasonic atomizer works
When, ultrasonic atomizatio piece is driven during sampling sheet concussion, and ultrasonic atomizatio room bottom baffles are set on
State, it is to avoid the interference that ultrasonic vibration brings, after concussion process terminates, ultrasonic atomizatio room bottom baffles
Closed state is set to off, headspace sampling analysis is carried out.
7. the application of the arbitrary on-line detector of a kind of claim 1-6, it is characterised in that：It is described
On-line detector is used in blood in the detection process of propofol agent.
8. the application of on-line detector according to claim 7, it is characterised in that：Blood sample
Ultrasonic atomizatio time control is in 1-10S.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510736073.1A CN106645367A (en) | 2015-11-03 | 2015-11-03 | Online detector combining ultrasonic atomization and ion mobility spectrometry and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510736073.1A CN106645367A (en) | 2015-11-03 | 2015-11-03 | Online detector combining ultrasonic atomization and ion mobility spectrometry and application |
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| CN106645367A true CN106645367A (en) | 2017-05-10 |
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| CN201510736073.1A Pending CN106645367A (en) | 2015-11-03 | 2015-11-03 | Online detector combining ultrasonic atomization and ion mobility spectrometry and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109839421A (en) * | 2017-11-27 | 2019-06-04 | 中国科学院大连化学物理研究所 | The method quickly detected for the direct mass spectrography of semi-volatile organic matter in liquid |
| CN110208358A (en) * | 2019-05-21 | 2019-09-06 | 暨南大学 | A kind of high-frequency vibration atomization ionization probe device and method |
| CN111220678A (en) * | 2018-11-23 | 2020-06-02 | 中国科学院大连化学物理研究所 | Ion mobility spectrometer |
| CN112086339A (en) * | 2019-06-13 | 2020-12-15 | 中国科学院大连化学物理研究所 | Ultrasonic atomization sample injector for ion mobility spectrometry |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005172692A (en) * | 2003-12-12 | 2005-06-30 | National Institute For Agro-Environmental Science | Trace liquid continuous atomization device and standard gas generation device using it |
| CN103884771A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Accurate method for detecting propofol anesthetic in blood |
| CN104716001A (en) * | 2013-12-13 | 2015-06-17 | 中国科学院大连化学物理研究所 | Liquid continuous sampling device for heating and gasification |
-
2015
- 2015-11-03 CN CN201510736073.1A patent/CN106645367A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005172692A (en) * | 2003-12-12 | 2005-06-30 | National Institute For Agro-Environmental Science | Trace liquid continuous atomization device and standard gas generation device using it |
| CN103884771A (en) * | 2012-12-21 | 2014-06-25 | 中国科学院大连化学物理研究所 | Accurate method for detecting propofol anesthetic in blood |
| CN104716001A (en) * | 2013-12-13 | 2015-06-17 | 中国科学院大连化学物理研究所 | Liquid continuous sampling device for heating and gasification |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109839421A (en) * | 2017-11-27 | 2019-06-04 | 中国科学院大连化学物理研究所 | The method quickly detected for the direct mass spectrography of semi-volatile organic matter in liquid |
| CN111220678A (en) * | 2018-11-23 | 2020-06-02 | 中国科学院大连化学物理研究所 | Ion mobility spectrometer |
| CN110208358A (en) * | 2019-05-21 | 2019-09-06 | 暨南大学 | A kind of high-frequency vibration atomization ionization probe device and method |
| CN112086339A (en) * | 2019-06-13 | 2020-12-15 | 中国科学院大连化学物理研究所 | Ultrasonic atomization sample injector for ion mobility spectrometry |
| CN112086339B (en) * | 2019-06-13 | 2021-06-15 | 中国科学院大连化学物理研究所 | Ultrasonic atomization sample injector for ion mobility spectrometry |
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Application publication date: 20170510 |