CN106644666A - Method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples - Google Patents
Method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples Download PDFInfo
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- CN106644666A CN106644666A CN201710073748.8A CN201710073748A CN106644666A CN 106644666 A CN106644666 A CN 106644666A CN 201710073748 A CN201710073748 A CN 201710073748A CN 106644666 A CN106644666 A CN 106644666A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The invention discloses a method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples. The method comprises the following steps of sample preparation, extraction, and pigment removal. The method has the advantages that the pigment removal method is improved, so that the absorbent nanometer activated carbon is replaced in a targeted way to overcome the defects in the prior art, and the pigment removal effect is better; the usage amount of adsorbent is greatly reduced, and the treatment time is shortened.
Description
Technical field
The invention belongs to the residual detection technique field of agriculture, and in particular to a kind of fruits and vegetables sample agriculture is residual to detect that depigmentation is removed in pre-treatment
Method.
Background technology
At present, the residual detection pre-treatment of fruits and vegetables sample agriculture goes the method for depigmentation to generally use tri- kinds of business of PSA, C18, GCB
Material single use or is used in mixed way as adsorbent, and its adsorbent amount is larger, goes the effect of depigmentation general, and locates
The reason time is longer.
The content of the invention
It is an object of the invention to provide a kind of fruits and vegetables sample agriculture is residual to detect the method that depigmentation is removed in pre-treatment, tradition is overcome
The deficiency of method, strengthens pigment removal effect, while reducing the consumption of adsorbent, shortens process time.
To reach above-mentioned purpose, the present invention provides following technical scheme：
A kind of fruits and vegetables sample agriculture is residual to detect the method that depigmentation is removed in pre-treatment, comprises the following steps：
（1）Sample preparation：After by fruits and vegetables sample clean, its edible part is cut into small pieces, is put into electric homogenate machine fully even
After slurry, less than -18 DEG C are deposited standby；
（2）Extract：By step（1）The 5-15g fruits and vegetables homogenised samples of preparation are added in centrifuge tube, are subsequently adding 5-15mL second
Nitrile, vortex 30-60s adds 5-10g NaCl, after vortex 1-3min, 5000r/min centrifugation 2-5min；
（3）Pigment is removed：By step（2）5-10ml acetonitrile layers after centrifugation are transferred in centrifuge tube, add 100-200mg anhydrous
Magnesium sulfate and 5-15mg active carbon nanoparticles, vortex 2-5min is gone after depigmentation, 5000r/min centrifugation 2-5min.
The step（3）In, active carbon nanoparticles are wood activated charcoal made by citrus peel residue, and its granularity is 100-200 mesh.
The beneficial effects of the present invention is：
Present invention improves over going the method for depigmentation, and more targeted replacement adsorbent active carbon nanoparticles are used, from
And overcome the deficiency of conventional method, pigment removal effect more preferably, while greatly reducing the consumption of adsorbent（Reduce 10-
50 times）, shorten process time（Shorten 5 times）.
Description of the drawings
Fig. 1 is the photo of embodiment 1 and the sample extracting solution after comparative example 1-3 process；
Fig. 2 is the UV-visible spectrum of embodiment 1 and the sample extracting solution after comparative example 1-3 process.
Specific embodiment
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, below by the preferred reality to the present invention
Apply example to be described in detail.
Embodiment 1
A kind of fruits and vegetables sample agriculture is residual to detect the method that depigmentation is removed in pre-treatment, comprises the following steps：
（1）Sample preparation：After by fruits and vegetables sample clean, its edible part is cut into small pieces, is put into electric homogenate machine fully even
After slurry, less than -18 DEG C are deposited standby；
（2）Extract：By step（1）The 10g fruits and vegetables homogenised samples of preparation are added in centrifuge tube, are subsequently adding 10mL acetonitriles, whirlpool
Rotation 40s, adds 10g NaCl, after vortex 2min, 5000r/min centrifugation 3min；
（3）Pigment is removed：By step（2）10mL acetonitrile layers after centrifugation are transferred in centrifuge tube, add 150mg anhydrous magnesium sulfates
With 10mg active carbon nanoparticles, vortex 3min gone after depigmentation, 5000r/min centrifugation 3min.
The step（3）In, active carbon nanoparticles are wood activated charcoal made by citrus peel residue, and its granularity is 150 mesh.
Comparative example 1-3
Comparative example 1-3 is the method that depigmentation is removed in the residual detection pre-treatment of traditional fruits and vegetables sample agriculture, respectively using C18 materials
500mg, PSA material 500mg, GCB material 50mg are used as adsorbent.
Fig. 1 is the photo of embodiment 1 and the sample extracting solution after comparative example 1-3 process, and numbering 1 is grape former base matter in figure
Extract, numbering 2 is comparative example 1, and numbering 3 is comparative example 2, and numbering 4 is comparative example 3, and numbering 5 is embodiment 1.Can be with from figure
Find out, the sample extracting solution Jing after the process of embodiment 1 is more as clear as crystal, and pigment residue is few, and pigment removal effect is more preferable.
Fig. 2 is the UV-visible spectrum of the sample extracting solution after embodiment 1 and comparative example 1-3 are processed, can be with from figure
Find out, in the wave-length coverage of 400-700nm, the absorbance of the sample extracting solution Jing after the process of embodiment 1 is minimum, shows reality
The pigment removal effect for applying example 1 is best.
The contrast more than is as can be seen that relative to conventional method, method of the present invention pigment removal effect is more preferable, adsorbent
Consumption only need 10mg, greatly reduce the consumption of adsorbent.
Finally illustrate, preferred embodiment above is only unrestricted to illustrate technical scheme, although logical
Cross above preferred embodiment to be described in detail the present invention, it is to be understood by those skilled in the art that can be
In form and in details various changes are made to it, without departing from claims of the present invention limited range.
Claims (2)
1. the method that depigmentation is removed in a kind of residual detection pre-treatment of fruits and vegetables sample agriculture, it is characterised in that：Comprise the following steps：
（1）Sample preparation：After by fruits and vegetables sample clean, its edible part is cut into small pieces, is put into electric homogenate machine fully even
After slurry, less than -18 DEG C are deposited standby；
（2）Extract：By step（1）The 5-15g fruits and vegetables homogenised samples of preparation are added in centrifuge tube, are subsequently adding 5-15mL second
Nitrile, vortex 30-60s adds 5-10g NaCl, after vortex 1-3min, 5000r/min centrifugation 2-5min；
（3）Pigment is removed：By step（2）5-10ml acetonitrile layers after centrifugation are transferred in centrifuge tube, add 100-200mg anhydrous
Magnesium sulfate and 5-15mg active carbon nanoparticles, vortex 2-5min is gone after depigmentation, 5000r/min centrifugation 2-5min.
2. the method that depigmentation is removed in the residual detection pre-treatment of fruits and vegetables sample agriculture according to claim 1, it is characterised in that：It is described
Step（3）In, active carbon nanoparticles are wood activated charcoal made by citrus peel residue, and its granularity is 100-200 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710073748.8A CN106644666A (en) | 2017-02-10 | 2017-02-10 | Method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710073748.8A CN106644666A (en) | 2017-02-10 | 2017-02-10 | Method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples |
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| CN106644666A true CN106644666A (en) | 2017-05-10 |
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| CN201710073748.8A Pending CN106644666A (en) | 2017-02-10 | 2017-02-10 | Method for removing pigment by pretreatment in agricultural residue detection of fruit and vegetable samples |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389819A (en) * | 2017-07-21 | 2017-11-24 | 中国农业科学院农业质量标准与检测技术研究所 | A kind of pre-treatment material, its preparation method and application and a kind of detection method of antibiotic |
| CN108693003A (en) * | 2018-04-26 | 2018-10-23 | 许昌学院 | A kind of method of leafy vegetable Detecting Pesticide sample pre-treatments |
Citations (4)
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|---|---|---|---|---|
| CN103266013A (en) * | 2013-05-20 | 2013-08-28 | 山东沂蒙山花生油股份有限公司 | Method for removing toxins from peanut oil |
| CN203324075U (en) * | 2013-06-19 | 2013-12-04 | 中国科学院新疆生态与地理研究所 | Activated carbon filtration purification device |
| CN106018048A (en) * | 2016-05-18 | 2016-10-12 | 潍坊汇海农产品检测有限公司 | Pretreatment method for detection of multiple pesticide residues in fruit and vegetable samples |
| CN106185930A (en) * | 2016-07-20 | 2016-12-07 | 西南大学 | A kind of preparation method of high methylene blue adsorption number carbon content active |
-
2017
- 2017-02-10 CN CN201710073748.8A patent/CN106644666A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103266013A (en) * | 2013-05-20 | 2013-08-28 | 山东沂蒙山花生油股份有限公司 | Method for removing toxins from peanut oil |
| CN203324075U (en) * | 2013-06-19 | 2013-12-04 | 中国科学院新疆生态与地理研究所 | Activated carbon filtration purification device |
| CN106018048A (en) * | 2016-05-18 | 2016-10-12 | 潍坊汇海农产品检测有限公司 | Pretreatment method for detection of multiple pesticide residues in fruit and vegetable samples |
| CN106185930A (en) * | 2016-07-20 | 2016-12-07 | 西南大学 | A kind of preparation method of high methylene blue adsorption number carbon content active |
Non-Patent Citations (4)
| Title |
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| EDA KÖSEOĞLU ET AL.: "Preparation, structural evaluation and adsorptive properties of activated carbon from agricultural waste biomass", 《ADVANCED POWDER TECHNOLOGY》 * |
| 余明池等: "气相色谱法测定水果和蔬菜中12种有机磷残留", 《公共卫生与预防医学》 * |
| 易路遥等: "气质联用法测定叶菜类蔬菜中8种农药残留", 《中国食品卫生杂志》 * |
| 王玉健等: "高效液相色谱-串联质谱法测定果蔬中6种三嗪类除草剂的残留量", 《理化检验-化学分册》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389819A (en) * | 2017-07-21 | 2017-11-24 | 中国农业科学院农业质量标准与检测技术研究所 | A kind of pre-treatment material, its preparation method and application and a kind of detection method of antibiotic |
| CN108693003A (en) * | 2018-04-26 | 2018-10-23 | 许昌学院 | A kind of method of leafy vegetable Detecting Pesticide sample pre-treatments |
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Application publication date: 20170510 |