CN106635826A - Preparation method of edible fungi cultivation strain based on recycling of disposable bamboo chopsticks - Google Patents
Preparation method of edible fungi cultivation strain based on recycling of disposable bamboo chopsticks Download PDFInfo
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- CN106635826A CN106635826A CN201610972667.7A CN201610972667A CN106635826A CN 106635826 A CN106635826 A CN 106635826A CN 201610972667 A CN201610972667 A CN 201610972667A CN 106635826 A CN106635826 A CN 106635826A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a method for preparing an edible fungi cultivation strain based on recycling of disposable bamboo chopsticks. According to the method, waste bamboo chopsticks are pre-processed in accordance with a 'fluffing' process, and a bamboo chopstick breeding bag is inoculated with liquid mycelia instead of a solid culture, in combination with an edible fungi tree fungus culture technique, so that a strain preparation process, which combines 'liquid strain and bamboo chopsticks', is obtained for preparing the edible fungi production strain. On one side, shortcomings of an original liquid strain technique which is difficult to operate and can cause pollution easily are overcome, and on the other side, the quality and production efficiency of a conventional tree strain are improved; by inoculating the breeding bag with the liquid mycelia instead of the solid culture, the production cycle of the breeding bag is shortened to 2/3 of an original production cycle; and the strain has the advantages of good production performance and high germination speed.
Description
Technical field
The present invention relates to a kind of preparation method of cultivation strain, specifically a kind of food based on disposable bamboo chopsticks regeneration
With the preparation method of bacterium tree fungus.
Background technology
Edible Fungi is nuisanceless, pure natural, capable of circulation, renewable, environmental protection.Edible mushroom has become traditional agriculture production neck
The sixth-largest ancestor's agricultural product after domain relaying grain, cotton, oil, fruit, dish；Mushroom industry has become existing in Derived from Agricultural Modernization
The soldier at the head of a formation and main force for agricultural development；The Hou Zhengxiang of Development Research Center of the State Council claims, and is as rising industry edible mushroom
The grain, cotton and oil industry of China will be exceeded, more than the automobile industry of China.
Into the new century, China's Edible Fungi technology development is maked rapid progress, and is constantly broken through in many technical fields, just by
Edible mushroom big country to edible mushroom power strides forward.But edible fungus culturing bacterial classification preparing technique process but perplexs all the time edible mushroom life
Produce.
It is long that reason is that " solid spawn " (including the tree fungus) commonly used at present production have a production of hybrid seeds cycle, hidden
Contact scar probability is big, and cell age is uneven and is difficult to the drawbacks such as standardized management, causes producer's cost to raise, and production risk is increased.
" liquid spawn " technology production of hybrid seeds cycle time that in recent years popularity is used, cell age is neat, and production efficiency is high, compares conventional solid
Bacterial classification has a clear superiority.
However, because liquid spawn has high demands to producing installations and facilities condition, will to the operation professional standards of producers
Ask high, the technical conditions to transporting and store have high demands, be relatively adapted to scale, the factorial praluction application of mechanization, and it is right
For traditional labour intensive production model that at present country still occupies the majority, there is greater risk using liquid spawn.From upper
From the nineties in century, many scientific research institutions of China, enterprise overall technology, equipment investment imperfection, it is jejune under the conditions of, it is anxious
In popularization and application edible fungi liquid strain, cause pollution in wide area, most idlenesses of equipment, waste, cause domestic edible mushroom
Produce and the application of liquid spawn is assumed an ambiguous attitude, pass different judgements on, the producer has to and returns " solid spawn " epoch.Therefore I
State's edible fungi liquid strain technology of preparing is urgently innovated and upgraded.
Therefore, a kind of edible fungus species preparation method by liquid spawn technology in combination with solid spawn technique is developed,
The comprehensive advantage that integrated edible fungi liquid strain is energetic, growth is fast and solid spawn is easy to maintain, easy to use, is not only production
Need, be also feasible.
Disposable bamboo chopsticks are huge in China's usage quantity, discarded object is become mostly and is polluted and is wasted.And the master of bamboo chopsticks
Want composition to be cellulose, be suitable nutrient matrix for edible fungi growth, can be used to the load as culture edible mushroom
Body, especially prepares the good material of edible mushroom tree fungus.Tree fungus are that a class commonly used in Edible Fungi is consolidated
Body bacterial classification form.
The content of the invention
It is an object of the invention to provide a kind of preparation side of the edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration
Method, the method increases strain quality and production efficiency.
In order to reach above-mentioned technical purpose, the technical scheme is that：
A kind of preparation method of the edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration, comprises the following steps：
(1) bamboo chopsticks inhale bamboo chopsticks permeable through cleaning, then by bamboo chopsticks hacking, make bamboo chopsticks surface roughening；
(2) edible mushroom strainses are inoculated into PDA slant mediums carries out activation culture；
(3) fluid nutrient medium is configured, fluid nutrient medium includes glucose 2.00g～2.50g/100mL, YE
0.20g～0.30g/100mL, potassium dihydrogen phosphate 0.20g～0.25g/100mL, magnesium sulfate 0.10g～0.15g/100mL, 121
DEG C sterilizing 20min.Fluid nutrient medium preferably includes glucose 2.00g/100mL, YE 0.20g/100mL, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.25g/100mL, magnesium sulfate 0.15g/100mL.
(4) edible mushroom mycelium activated in PDA slant mediums is inoculated in fluid nutrient medium.
(5) it is 24～26 DEG C in fermentation temperature, preferably 25 DEG C, shaking speed is that (shaking speed is preferred for 160～180r/min
180r/min) condition bottom fermentation, fermentation time is 6d.
(6) the liquid mycelium pellet for fermenting is smashed, with sterilized water extension rate, 300-500 times obtains liquid spawn.
(7) auxiliary material is cultivated with production of hybrid seeds bag, the kind bag cultivation auxiliary material includes following mass percent component：Wood chip 40%,
Cotton seed hulls 50%, wheat bran 8%, land plaster 1% and pulverized limestone 1%.Above-mentioned kind bag cultivation auxiliary material adds water to 60% water content.
(8) plant in bag cultivation auxiliary material and be booked the bamboo chopsticks handled well, quantity is 120-140 bamboo chopsticks/bag, the gap of bamboo chopsticks and
Surface is covered with auxiliary material.
(9) kind of a bag is put into steam sterilization pan and is sterilized, bamboo chopsticks are sterilized under 0.103MPa, 121 DEG C of steam pressure
40-50 minutes；After the completion of sterilizing, bag bag temperature drop to less than 33 DEG C, the good edible fungus liquid mycelia of inoculated and cultured, inoculum concentration is
20-25mL/ bags.
In edible mushroom technical research and production application, the sterilising conditions of general solid matrix (bamboo chopsticks etc.) are
0.138MPa, sterilizes 120 minutes or so by 126 DEG C, this sterilising conditions time consumption and energy consumption, and causes bamboo chopsticks matrix nutrition composition
Loss.The sterilizing parameter of the present invention can not only play good sterilization effect, and reduce bamboo chopsticks matrix nutrition composition
Loss and the energy waste, its know-why is：The characteristics of fast, growth is rapid is sprouted using liquid bacteria inoculation bamboo chopsticks branch
(liquid mycelia can rapidly be covered with bamboo chopsticks surface and internally grow, and make hypha of edible fungus grow state of having the advantage, so as to press down
Varied bacteria growing processed), while appropriate reduction sterilizing parameter, do not interfere with bamboo chopsticks surface hypha of edible fungus normal growth；It is another
Aspect, because the sterilizing parameter of the present invention reduces treatment temperature, shortens process time, so as to reduce bamboo chopsticks matrix nutrition
The loss of composition and the waste of the energy.
During liquid bacteria switching bamboo chopsticks branch bag, the size of inoculum concentration directly affects mycelium germination growth result after inoculation.Such as
Fruit inoculum concentration is little, and not only mycelial growth is slow, and longer growth cycle easily causes branch by living contaminants.If inoculum concentration
Greatly, the wasting of resources is not only caused, and hygienic conditions and refrigeration, ventilation equipment is had high demands, it is impossible to adaptive technique weak,
The not enough factory of bacterial classification technical staff, cannot particularly be perfectly suitable for distributing plantation peasant household.Importantly, inoculum concentration
Greatly, can branch surface formed one layer of bacterium mucous membrane, bacterium mucous membrane will hinder fresh air outside enter, this will cause internal mycelia because
Anoxic and cannot normal growth, ultimately causing miscellaneous bacteria infects, increase liquid spawn application risk.20-25mL/140 branches/bag
Inoculum concentration overcomes above-mentioned contradiction, on the basis of sprouting effect is guaranteed, reduces the waste of resource, it is to avoid in branch surface shape
Into one layer of bacterium mucous membrane, the entrance of fresh air will not be hindered, it is ensured that the regular supply of oxygen, it is ensured that the normal life of mycelia
It is long.
(10) cultivate under the conditions of the kind bag being inoculated with being placed in into 26 DEG C, after mycelia long purseful, continue to cultivate 3-5 days,
Bamboo chopsticks in bag are made to cover with mycelia.
Edible fungi liquid strain is replaced solid culture inoculation bamboo chopsticks by the present invention, with reference to edible mushroom tree fungus culture skill
Art, forms the edible fungus species preparation technology of " liquid spawn+bamboo chopsticks ", on the one hand improves original liquid spawn technical operation
Difficulty, the easily deficiency of pollution, while improve traditional tree fungus quality and production efficiency, foreshorten to the tree fungus production cycle
2/3 originally, the production traits without being decreased obviously, and with sprouting fast advantage.
Specific embodiment
Test strain：Pleurotus eryngii.
PDA solid mediums：Potato 200g, glucose 20g, agar 20g, water 1000mL, pH natures.
(1) disposable bamboo chopsticks (such as waste and old disposable bamboo chopsticks) are cut into long 7.5cm, 0.5 centimeter of strip of diameter, bamboo chopsticks are passed through
Cleaning, inhales bamboo chopsticks permeable, then by bamboo chopsticks hacking, makes bamboo chopsticks surface roughening.
(2) edible mushroom strainses are inoculated into PDA slant mediums carries out activation culture.
(3) fluid nutrient medium is configured, fluid nutrient medium includes glucose 2.00g/100mL, YE 0.20g/
100mL, potassium dihydrogen phosphate 0.25g/100mL, magnesium sulfate 0.15g/100mL, 121 DEG C of sterilizing 20min.
(4) edible mushroom mycelium activated in PDA slant mediums is inoculated in fluid nutrient medium.
(5) it is 25 DEG C in fermentation temperature, shaking speed is 180r/min condition bottom fermentations, and fermentation time is 6d.
Under aforesaid liquid culture medium and fermentation condition, fermentation mycelium biomass (dry weight) can reach 1.93g/100mL,
Output increased 1.24 times.
(6) the liquid mycelium pellet for fermenting is smashed, with 500 times of acquisition liquid spawns of sterilized water extension rate.
Under the conditions of different dilution factors, when inoculum concentration is 10mL/ bags, the speed of growth of mycelia is relatively slow for pure culture,
When inoculum concentration is that 20mL/ bags and mycelial growth rate during 30mL/ bags are basically identical, all covering with cultivating bag on the same day, and
And its mycelial growth rate is both greater than speed of growth when inoculum concentration is 10mL/ bags.The mycelial growth speed of the bacterial classification of 500 times of dilution
It is also most fast that degree is relative.So in general, the optimum diluting multiple of pure culture is 500 times.
(7) auxiliary material is cultivated with production of hybrid seeds bag, the kind bag cultivation auxiliary material includes following mass percent component：Wood chip 40%,
Cotton seed hulls 50%, wheat bran 8%, land plaster 1% and pulverized limestone 1%.Above-mentioned kind bag cultivation auxiliary material adds water to 60% water content.
(8) plant in bag cultivation auxiliary material and be booked the bamboo chopsticks handled well, quantity is 140 bamboo chopsticks/bags, the gap and surface of bamboo chopsticks
Covered with kind of a bag cultivation auxiliary material.
(9) kind of a bag is put into steam sterilization pan and is sterilized, bamboo chopsticks are sterilized under 0.103MPa, 121 DEG C of steam pressure
50 minutes；After the completion of sterilizing, bag temperature drop to less than 33 DEG C is treated, the good edible fungus liquid mycelia of inoculated and cultured, inoculum concentration is 25mL/
Bag.
When inoculum concentration for 20mL/ bags when, the growth of mycelia and inoculum concentration for 25mL/ bags when without significantly distinguishing, basic phase
When.
(10) cultivate under the conditions of the kind bag being inoculated with being placed in into 26 DEG C, after mycelia long purseful, continue to cultivate 3-5 days,
Bamboo chopsticks in bag are made to cover with mycelia.
After mycelia long purseful, continue culture and be enlarged within 3-5 days cultivation；, make to cover with mycelia in bag inside bamboo chopsticks.
Because liquid spawn growth is rapid, after inoculation bamboo chopsticks, bamboo chopsticks production of hybrid seeds bag is sent out bacterium speed and is substantially accelerated.Using tradition system
The standby technique productions branch kind cycle is 30-40 days, utilizes " liquid spawn+bamboo chopsticks " technology, and the bacterial classification production cycle is 20-25 days.
Above-described embodiment limits never in any form the present invention, every to be obtained by the way of equivalent or equivalent transformation
Technical scheme all fall within protection scope of the present invention.
Claims (4)
1. a kind of preparation method of the edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration, it is characterised in that including following
Step：
(1) bamboo chopsticks inhale bamboo chopsticks permeable through cleaning, then by bamboo chopsticks hacking, make bamboo chopsticks surface roughening；
(2) edible mushroom strainses are inoculated into PDA slant mediums carries out activation culture；
(3) fluid nutrient medium is configured, fluid nutrient medium includes glucose 2.00g～2.50g/100mL, YE 0.20g
～0.30g/100mL, potassium dihydrogen phosphate 0.20g～0.25g/100mL, magnesium sulfate 0.10g～0.15g/100mL, 121 DEG C of sterilizings
20min；
(4) edible mushroom mycelium activated in PDA slant mediums is inoculated in fluid nutrient medium；
(5) it is 24～26 DEG C in fermentation temperature, shaking speed is 160～180r/min condition bottom fermentations, and fermentation time is 6d；
(6) the liquid mycelium pellet for fermenting is smashed, with sterilized water extension rate, 300-500 times obtains liquid spawn；
(7) auxiliary material is cultivated with production of hybrid seeds bag, and adds water to 60% water content；
(8) plant and the bamboo chopsticks handled well are booked in bag cultivation auxiliary material, bamboo chopsticks quantity is that 120-140 props up/bag, the gap and surface of bamboo chopsticks
Covered with kind of a bag cultivation auxiliary material；
(9) kind of a bag is put into steam sterilization pan and is sterilized, to bamboo chopsticks sterilizing 40-50 under 0.103MPa, 121 DEG C of steam pressure
Minute；After the completion of sterilizing, bag temperature drop to less than 33 DEG C is treated, the good edible fungus liquid mycelia of inoculated and cultured, inoculum concentration is 20-
25mL/ bags；
(10) cultivate under the conditions of the kind bag being inoculated with being placed in into 26 DEG C, after mycelia long purseful, continue to cultivate 3-5 days, make bag
Interior bamboo chopsticks cover with mycelia.
2. the preparation side of a kind of edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration according to claim 1
Method, it is characterised in that：The bag cultivation auxiliary material of planting includes following mass percent component：Wood chip 40%, cotton seed hulls 50%, wheat bran
8%th, land plaster 1% and pulverized limestone 1%.
3. the preparation side of a kind of edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration according to claim 1
Method, it is characterised in that：In step (3), fluid nutrient medium includes glucose 2.00g/100mL, YE 0.20g/
100mL, potassium dihydrogen phosphate 0.25g/100mL, magnesium sulfate 0.15g/100mL.
4. the preparation side of a kind of edible fungus culturing bacterial classification based on disposable bamboo chopsticks regeneration according to claim 1
Method, it is characterised in that：In step (5), fermentation temperature is 25 DEG C, and shaking speed is 180r/min condition bottom fermentations.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN108858553A (en) * | 2018-08-07 | 2018-11-23 | 耒阳市奇宏林业有限公司 | A kind of bamboo chopsticks raising machine |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159554A (en) * | 2013-04-08 | 2013-06-19 | 杨光 | Method for preparing edible fungus culture medium by using disposable chopsticks |
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2016
- 2016-11-07 CN CN201610972667.7A patent/CN106635826A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103159554A (en) * | 2013-04-08 | 2013-06-19 | 杨光 | Method for preparing edible fungus culture medium by using disposable chopsticks |
Non-Patent Citations (4)
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| 徐兵等: "杏鲍菇还原型液体菌种应用技术研究", 《食品研究与开发》 * |
| 徐兵等: "白背毛木耳枝条菌种的制作", 《食品研究与开发》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN108858553A (en) * | 2018-08-07 | 2018-11-23 | 耒阳市奇宏林业有限公司 | A kind of bamboo chopsticks raising machine |
| CN108858553B (en) * | 2018-08-07 | 2023-09-22 | 耒阳市奇宏林业有限公司 | Bamboo chopsticks fuzzing machine |
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