CN106635767A - Sterile air-permeable sealing film structure for microbe culture bottle - Google Patents
Sterile air-permeable sealing film structure for microbe culture bottle Download PDFInfo
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- CN106635767A CN106635767A CN201510721333.8A CN201510721333A CN106635767A CN 106635767 A CN106635767 A CN 106635767A CN 201510721333 A CN201510721333 A CN 201510721333A CN 106635767 A CN106635767 A CN 106635767A
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- bottle
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- culture
- sealing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a sterile air-permeable sealing film structure for a microbe culture bottle. The structure can be flexibly regulated and adapted according to size specifications of different culture bottles; the upper part of an adapted culture bottle is provided with a bottle neck; a plastic film is bound by a binding tape or rubber band at the bottle neck of the culture bottle; a bottle neck seal is correspondingly placed at the opening of the bottle neck; the central position of the bottle neck seal is provided with a sealing film air-permeable window structure; the sealing film air-permeable window structure comprises a bottom layer film and a top layer film; a filtering medium is arranged between the bottom layer film and the top layer film; and the central positions of the upper and lower surfaces of the filtering medium are fixedly connected with the bottom layer film and the top layer film through center dispensing. The sealing structure can effectively filter and obstruct microbes in the air, and simultaneously can ensure the supply of the air in the culture bottle. Besides, the sealing structure can effectively prevent the filtering medium from producing a leak due to dislocation or folding deformation in the culture process, thereby ensuring the culture safety. The sealing structure is simple and convenient to use, hardly occupies the volume inside/outside the bottle, and improves the culture efficiency.
Description
Technical field
The invention belongs to microbial cultivation equipment technical field, the aseptic breathable sealing membrane structure of more particularly to a kind of microorganism culture flask.
Background technology
In microbial cultivation process is carried out, need to use blake bottle, blake bottle generally needs to carry out sealing operation, prevent the microorganisms such as extraneous bacterium, fungi from entering into bottle by sealing and pollute.At present conventional sealing mode is exactly directly to tie up the forms such as film, brown paper or the spherical plug of plug gauze, cotton and chemical fibre in bottleneck to carry out sealing operation, there is the defect of the following aspects using this several sealing mode：（1）Though the spherical plug of plug gauze, cotton and chemical fibre can meet gas permeability requirements, the easy moisture absorption of stopper is infected with miscellaneous bacteria, and the larger troublesome poeration of stopper volume, need to take certain bottleneck interior or exterior space.（2）Polypropylene, polyethylene film or brown paper sealing operation are simple, it is not take up bottleneck interior or exterior space, but do not have fine air permeability, have influence on bacterial classification and supply oxide growth, and bottleneck sealer often expands deformation because of inside and outside differential pressure after sterilizing, then easily there is fracture phenomena under damp and hot and pressure reduction situation in brown paper.（3）A kind of existing design sealed membrane on probation adopts three-decker, one layer of filter paper is set used as filter medium in two layers of plastic thin films interlayer, four symmetrical air-vents are processed in the upper and lower surface of two layers of plastic thin films, ensure ventilative, but pull inequality when during use due to the air pressure change or sealing operation inside blake bottle, will cause to produce displacement or forming corrugations between the filter paper of bottleneck and film, so that the filtered media filtration of the air entered into inside blake bottle and be directly entered from air-vent gap, good filter bacteria is not had, the purpose of the external microbes such as fungi, internal culture is caused to there is contaminated risk, additionally, due to upper and lower two layers of plastic thin films without fixed well, it is also easy to cause its laterally zygomorphic air-vent not correspond to, the unimpeded of air cannot be ensured, so as to affect the effect of its culture supply.
The content of the invention
The purpose of the present invention is aiming at above-mentioned technological deficiency, the advantage of comprehensive stopper and film, there is provided a kind of microorganism culture flask aseptic transom sealing membrance structure, enclosuring structure can effectively filter the microorganism in outside air, ensure the supply of blake bottle internal oxygen simultaneously, the dislocation of filter medium during use can also effectively be prevented, it is ensured that culture security, improve culture efficiency.
To reach above-mentioned purpose, the design of the present invention is：A kind of aseptic breathable sealing membrane structure of microorganism culture flask, it includes the blake bottle for adapting to, bottleneck is provided with the top of blake bottle, the bottleneck of blake bottle has tied up plastic sheeting by band, the opening of bottleneck is provided with bottle sealing, the center of bottle sealing is provided with the ventilative window construction of sealed membrane, the ventilative window construction of the sealed membrane includes bottom film and top film, filter medium is provided between bottom film and top film, on filter medium, the center of lower surface is fixedly linked by center dispensing with bottom film and top film, the bottom film and top film are all machined with uniform annular through-hole at its center certain diameter, on, lower annular through-hole is corresponding, in the inner side of annular through-hole, there are annular dispensing and outer shroud form point glue respectively at the certain diameter of outside.
The bottom film and top film are made using polyethylene or polypropylene material.
The filter medium is fixed by annular dispensing and outer shroud form point glue with bottom film and top film simultaneously.
The filter medium is made using can tolerate the sterilization grade filter paper diaphragm of 100 DEG C of -127 DEG C of high-temperature sterilizations, nonwoven cloth material, being capable of air in filtration, purification bottle.
The band can be using tolerable 100 DEG C of rubber rings to 127 DEG C of high-temperature sterilizations.
The method have the advantages that：
When needing to seal adaptation blake bottle, plastic sheeting is tightened on bottleneck by being resistant to the band or elastic tape of 100 DEG C to 127 DEG C sterilizing high temperature, the center of bottle sealing is provided with the ventilative window construction of sealed membrane, enclosuring structure has three layers, there is filter medium in bottom film and top film middle setting, filter medium passes through outer shroud form point glue, annular dispensing and center dispensing are fixed, it is arranged on the annular through-hole on bottom film and top film, it is lower symmetrical, filter media resistance bacterium is can be transferred through so as to ensure that into all of air of blake bottle, so as to ensure that filter effect, improve culture efficiency, simultaneously the fixed effect that improve filter medium is fixed by multi-point adhesive, prevent from it to be subjected to displacement to produce leak and affect filter effect with forming corrugations, the reusable number of times of sealed membrane can also be increased, promote environmentally friendly and cost-effective.
Description of the drawings
With reference to the accompanying drawings and examples the invention will be further described.
Fig. 1 is overall structure diagram of the present invention.
Fig. 2 is the top view of sealing membrance structure of the present invention.
Fig. 3 is sealing membrance structure Section A-A figure of the present invention.
In figure：Blake bottle 1, plastic sheeting 2, band 3, bottleneck 4, bottle sealing 5, sealed membrane are breathed freely window construction 6, bottom film 601, filter medium 602, top film 603, outer shroud form point glue 604, annular through-hole 605, annular dispensing 606, center dispensing 607.
Specific embodiment
Embodiments of the present invention are described further below in conjunction with the accompanying drawings.
Participate in Fig. 1-3, a kind of aseptic breathable sealing membrane structure of microorganism culture flask, it includes the blake bottle 1 for adapting to, the top of blake bottle 1 is provided with bottleneck 4, the bottleneck 4 of blake bottle 1 has tied up plastic sheeting 2 by band 3, the opening of bottleneck 4 is provided with bottle sealing 5, the center of bottle sealing 5 is provided with the ventilative window construction 6 of sealed membrane, the ventilative window construction 6 of the sealed membrane includes bottom film 601 and top film 603, filter medium 602 is provided between bottom film 601 and top film 603, on filter medium 602, the center of lower surface is fixedly linked by center dispensing 607 with bottom film 601 and top film 603, the bottom film 601 and top film 603 are all machined with uniform annular through-hole 605 at its center certain diameter, on, lower annular through-hole 605 is corresponding, in the inner side of annular through-hole 605, there are annular dispensing 606 and outer shroud form point glue 604 respectively at the certain diameter of outside.
Further, the bottom film 601 and top film 603 are made using polyethylene or polypropylene material.The structural strength of sealing ensure that using polythene material or polypropylene material, extend its service life.
Further, the filter medium 602 is fixed by annular dispensing 606 and outer shroud form point glue 604 with bottom film 601 and top film 603 simultaneously.The stability that filter medium 602 is fixed with bottom film 601 and top film 603 is improve by multiple spot annular dispensing, is prevented it to be subjected to displacement or is deformed gauffer and produce gap.
Further, the quantity of the annular through-hole 605 can be increased and decreased according to the number of throughput.Different throughputs can be provided by the annular through-hole 605 of varying number.
Further, the filter medium 602 is using sterilization grade filter paper diaphragm, the non-woven fabrics that can tolerate 100 DEG C to 127 DEG C high-temperature sterilizations.The external microbe in air can be effectively prevented to pollute into bottle so as to ensure that.
The present invention the course of work and operation principle be：
When making sealing membrance structure 6, first the correspondence position of bottom film 601 and top film 603 is processed into laterally zygomorphic annular through-hole 605, then filter medium 602 is arranged between bottom film 601 and top film 603, and its center is carried out into dispensing fix, center line dispensing carries out again inner ring dispensing and forms annular dispensing 606 after fixing, then outer shroud form point glue 604 is carried out, ensure that bottom film 601 is corresponding with the annular through-hole of top film 603 during dispensing, whole plastic sheeting 2 and sealing membrance structure 6 are fixed on the bottleneck for adapting to blake bottle 1 by band 3 during use.
Using said structure, air is first by the annular through-hole 605 of top film 603, pass through filter medium 602 again, it is last to be entered into inside blake bottle 1 by the annular through-hole 605 of bottom film 601 again, the air now entered into inside blake bottle 1 all carries out hindering bacterium filtration through filter medium 602, it is ensured that air clean hygiene, is fixed by multiple spot annular dispensing, it is effectively prevented filter medium 602 and is subjected to displacement or deforms gauffer generation Air Leakage Defect, it is ensured that its stability.
Embodiments of the present invention are explained in detail with reference to accompanying drawing, but the present invention is not limited to above-mentioned embodiment.Technical staff in claims, can modify in the innovative point and operating procedure without prejudice to the present invention to above-described embodiment.Protection scope of the present invention, should cover such as claims of the present invention.
Claims (6)
1. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask, it includes the blake bottle for adapting to（1）, blake bottle（1）Top be provided with bottleneck（4）, it is characterised in that：Blake bottle（1）Bottleneck（4）By band（3）Plastic sheeting is tied up（2）, bottleneck（4）Opening be provided with bottle sealing（5）, bottle sealing（5）Center be provided with the ventilative window construction of sealed membrane（6）, the sealed membrane breathes freely window construction（6）Including bottom film（601）And top film（603）, in bottom film（601）And top film（603）Between be provided with filter medium（602）, filter medium（602）The center on upper and lower surface passes through center dispensing（607）With bottom film（601）And top film（603）It is fixedly linked, the bottom film（601）And top film（603）Uniform annular through-hole is all machined with its center certain diameter（605）, upper and lower annular through-hole（605）It is corresponding, in annular through-hole（605）Inner side, have annular dispensing respectively at the certain diameter of outside（606）With outer shroud form point glue（604）.
2. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask according to claim 1, it is characterised in that：The bottom film（601）And top film（603）Made using polyethylene or polypropylene material.
3. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask according to claim 1, it is characterised in that：The filter medium（602）Pass through annular dispensing simultaneously（606）With outer shroud form point glue（604）With bottom film（601）And top film（603）It is fixed.
4. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask according to claim 1, it is characterised in that：The annular through-hole（605）Quantity can be increased and decreased according to the number of throughput.
5. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask according to claim 1, it is characterised in that：The filter medium（602）Made using can tolerate the sterilization grade filter paper diaphragm of 100 DEG C of -127 DEG C of high-temperature sterilizations, nonwoven cloth material, being capable of air in filtration, purification bottle.
6. the aseptic breathable sealing membrane structure of a kind of microorganism culture flask according to claim 1, it is characterised in that：The band（3）Can be using tolerable 100 DEG C of rubber rings to 127 DEG C of high-temperature sterilizations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510721333.8A CN106635767A (en) | 2015-10-30 | 2015-10-30 | Sterile air-permeable sealing film structure for microbe culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510721333.8A CN106635767A (en) | 2015-10-30 | 2015-10-30 | Sterile air-permeable sealing film structure for microbe culture bottle |
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| Publication Number | Publication Date |
|---|---|
| CN106635767A true CN106635767A (en) | 2017-05-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510721333.8A Pending CN106635767A (en) | 2015-10-30 | 2015-10-30 | Sterile air-permeable sealing film structure for microbe culture bottle |
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| CN (1) | CN106635767A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094733A (en) * | 2020-10-19 | 2020-12-18 | 河南省科学院生物研究所有限责任公司 | A aseptic closing device that breathes freely of portable for laboratory tissue culture container |
| CN115594130A (en) * | 2022-12-13 | 2023-01-13 | 成都圣恩生物科技股份有限公司（Cn） | Automatic bottle opening packaging device and packaging method |
-
2015
- 2015-10-30 CN CN201510721333.8A patent/CN106635767A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094733A (en) * | 2020-10-19 | 2020-12-18 | 河南省科学院生物研究所有限责任公司 | A aseptic closing device that breathes freely of portable for laboratory tissue culture container |
| CN115594130A (en) * | 2022-12-13 | 2023-01-13 | 成都圣恩生物科技股份有限公司（Cn） | Automatic bottle opening packaging device and packaging method |
| CN115594130B (en) * | 2022-12-13 | 2023-03-10 | 成都圣恩生物科技股份有限公司 | Automatic bottle opening packaging device and packaging method |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |