CN106613826A - Low-potassium-tolerant cotton seedling nutrient solution and application thereof - Google Patents
Low-potassium-tolerant cotton seedling nutrient solution and application thereof Download PDFInfo
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- CN106613826A CN106613826A CN201610986192.7A CN201610986192A CN106613826A CN 106613826 A CN106613826 A CN 106613826A CN 201610986192 A CN201610986192 A CN 201610986192A CN 106613826 A CN106613826 A CN 106613826A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention provides a low-potassium-tolerant cotton seedling nutrient solution. The nutrient solution is prepared from 1.25-mM Ca(NO3)2, 0.5-mM MgSO4, 0.25-mM (NH4)H2PO4, 0.05-Mm Fe-Na-EDTA, 0.1-muM CuSO4, 0.5-muM ZnSO4, 10-muM H3BO3, 2.5-pM (NH4)6Mo7O24 and 0.5-muM MnSO4. The invention further provides application of the nutrient solution in low-potassium-tolerant cotton variety screening. According to the low-potassium-tolerant cotton seedling nutrient solution and application thereof, the nutrient solution concentration ratio causing the most significant low-potassium-tolerant difference of cotton seedlings is screened out under different potassium supply levels by taking a potassium-sensitive variety-new cotton 99<B> and a low-potassium-tolerant variety-Xinluhzong 61 as materials, a theoretical basis is provided for hydroponic culture of cotton, and meanwhile the important guiding significance on balanced nutrition fertilization is achieved.
Description
Technical field
The present invention relates to plant genetics and breeding field, specifically, be related to a kind of low-kalium resistant cotton seedling nutrient solution and its
Using.
Background technology
Chinese potassium resource shortage, most of cotton region soil is in lean potassium state, and deficiency of potassium becomes impact Chinese cotton peanut
One of main limiting factor of product (uncommon peak of model etc., 2006；Peng Long etc., 2011).Cotton is happiness potassium crop, to soil potassium deficiency more
Sensitive (Zhang et al, 2007；Wang et al.2012).In order to improve crop potassium nutrition efficiency and alleviate China's potassium
The present situation of shortage of resources, the plant genotype of seed selection low-kalium resistant is an effective way of " ecological environment-friendly type " agricultural sustainable development
Footpath.In the seed selection of potassium high-efficiency plant genotype, first have to solution is exactly the foundation of potassium high-efficiency crop screening technique, for not
It is to carry out the primary premise of this work to set up suitable screening technique and screening index with crop.Carry out potassium efficiently to screen
Can be generally divided into land for growing field crops, earth culture, sand culture and hydroponics；Wherein, using aquicultural method to cotton genotype tolarant to K deficiency
Type carry out screening have feasibility (Tian Xiaoli etc., 2008), and quick, the easily controllable nutrient solution concentration of the method simplicity etc., tool
There is incomparable advantage.
Hydroponics, also known as water planting, are to study the most basic and most useful method of the mineral nutrition of plant.In nutrient solution from
Sub- concentration (De Rijck and Schrevens, 1995) and ion proportioning (Nukaya et al, 1995) to plant
Grow most important, be the key of water planting success or not.When nutrient solution concentration is relatively low, it can be suppressed due to nutritional deficiency
Growth, and higher concentration then can cause plant occur osmotic stress (Pan Jingxian etc., 2005；Fourth is elegant etc., 2012), and appoints
A kind of shortage or excess of what element can all cause certain physiological disorder of crop or disease, and then affect the growth of crop
(Shimaet al,1996；Willumsen et al,1996).At present for the screening study work of nutrient solution mostly be it is identical from
Under sub- proportioning, and impact of the Different Nutrition liquid concentration to plant growth (Pan Jingxian etc., 2005；Chen Yonghua etc., 2007；Sun Hongyan etc.,
2008)；Under conditions of nutritional imbalance, the nutrient solution concentration proportion research report optimal to plant growth is less, to nutritional deficiency
Under the conditions of the screening of optimum nutrient solution concentration lack directive function.Also, cotton in seedling stage is carried out using the method for water planting resistance to
When low potassium is screened, its best nutritional liquid concentration proportioning is not yet seen in report.The present invention is with potassium sensitiveness kind-new cotton 99BWith it is resistance to
61 is material in low potassium kind-new land, under different potassium supply levels, filters out cotton seedling Ability of bearing low potassium difference the most notable
Nutrient solution concentration proportioning, provide theoretical foundation for cotton water planting culture, at the same to balanced nutrients fertilising also have important finger
Lead meaning.
The content of the invention
It is an object of the invention to provide a kind of low-kalium resistant cotton seedling nutrient solution.
It is a further object of the present invention to provide application of the nutrient solution in the screening of low-kalium resistant cotton variety.
In order to realize the object of the invention, the low-kalium resistant cotton seedling nutrient solution of the present invention, the formula of the nutrient solution is：
1.25mM Ca(NO3)2,0.5mM MgSO4,0.25mM(NH4)H2PO4,0.05mM Fe-Na-EDTA,0.1μM CuSO4,0.5μ
M ZnSO4,10μM H3BO3,2.5pM (NH4)6Mo7O24With 0.5 μM of MnSO4。
The present invention also provides application of the nutrient solution in cotton seedling water planting culture.
The present invention further provides application of the nutrient solution in the screening of low-kalium resistant cotton variety.
The application is by the Solution culture method of the cotton seedling containing different potassium concentrations (0.03-2.50mM)
After a period of time, according to the Root morphology of seedling, and by determining dry matter, the Ca of seedling2+、K+、Na+And Mg2+Level and profit
Low-kalium resistant cotton variety is screened with indexs such as rates.
Specifically, cotton seeds are sterile-processed, and after being rinsed with water, then presoaking and germinating is sowed up to showing money or valuables one carries unintentionally in water
In sand bed, seedling is transferred in the Solution culture method containing different potassium concentrations after 4d is cultivated to 4 leaf phases, sampling
Determine dry matter, ion concentration and the Ca of seedling2+、K+、Na+And Mg2+Utilization rate.
The present invention is comprised the following steps using the method that the nutrient solution screens low-kalium resistant cotton variety：
1) cotton seeds with 9% dioxygen water sterilization 30min, with water rinsing for several times after deionized water presoaking and germinating until
Show money or valuables one carries unintentionally, be seeded in the flushed sand bed of deionized water；Seedling is transferred to into the modeling that volume is 32cm × 26cm × 32cm after 4d
Ventilation culture is carried out in magazine, the nutrient solution containing different potassium concentrations is held in plastic casing, per box 6 plants of childrens are put into
Seedling, one time of nutrition liquid is changed per 4d；
2) cultivate to during 4 leaf phase and sample, with EPSON (Seiko Epson Corp., Tokyo, Japan) scanner scanning
Root morphology, with root system image analysis software Win Rhizo (version 4.0b, Regent Instruments Inc.,
Quebec, Canada 2000) analysis Root Parameters；
3) by seedling after 105 DEG C of 30min that complete, dry to constant weight at 80 DEG C；Pulverize and sieve after drying, use 1mol L- 1HCl extracts 5h, vibrates 30min, filters, and with atomic absorption spectrophotometer K is determined+、Na+、Ca2+And Mg2+Concentration；
4) ionic availability (ion utilization index) is calculated using following equation：Ionic availability (g2/ mg)=dry matter/
Ion concentration；
5) according to step 2) -4) result, with the suppressed rate of dry matter accumulation less than 20%, potassium ion utilization index increases
As criterion, low-kalium resistant cotton variety is filtered out for more than 4 times.
Aforesaid method, step 1) in the condition of culture of seedling be：450 μm of ol m of intensity of illumination-2s-1, during illumination/dark
A length of 14h/10h, cultivation temperature during illumination is 30 ± 2 DEG C, and cultivation temperature when dark is 20 ± 2 DEG C.
Low-kalium resistant cotton variety of the present invention is included 61 in new land, distant cotton 18, CCRI 4, Ji cotton 66 etc..
The present invention is with new cotton 99B61 (low-kalium resistant representative strains) are material in (potassium sensitive representations strain) and new land, be have studied
The Hoagland nutrient solution concentrations (NS) of 1,1/2 and 1/4 times of (0.03mM and 2.50mM) are to Young Cotton under different potassium supply levels
The impact of seedling growth.As a result show, during for potassium abundance, the NS of low concentration reduces dry matter, reduces Ca in seedling2+Utilization
Index and K+、Na+And Mg2+Accumulation, to Ca2+Accumulation and K+、Na+And Mg2+Utilization index do not make significant difference.It is low
Under the conditions of potassium, the NS of high concentration inhibits the growth of seedling, and growth conditions optimal between kind difference；New cotton 99BWith
61 respectively there is higher cation to accumulate and Utilization ability under the conditions of 1/4NS and 1/2NS in new land, maintain higher doing
Photosynthetic matter accumulation.Under different potassium supply levels, the change of nutrient solution concentration have impact on absorption and utilization of the plant to element so that cotton
Optimum nutrient solution concentration difference is grown between flower variety；Wherein 1/2NS conditions can be used as rational water planting culture bar
Part, i.e., can be used as the critical value for not affecting it to grow under for potassium sufficiency, can also be used as suitable under the conditions of low potassium
The concentration of growth.The present invention provides theoretical foundation for cotton water planting culture, while having important guidance to balanced nutrients fertilising
Meaning
Description of the drawings
Fig. 1 is the shadow in the embodiment of the present invention under different potassium supply levels to the accumulation of two kind cotton seedling dominant cations
Ring.
Fig. 2 is the shadow in the embodiment of the present invention under different potassium supply levels to the accumulation of two kind cotton seedling dominant cations
Ring.
Fig. 3 be in the embodiment of the present invention under different potassium supply levels to the shadow of the cation utilization index of two kind cotton seedlings
Ring.
In figure, different letters are represented in p<Significant difference in 0.05 level.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional meanses that are well known to those skilled in the art of technological means used, it is raw materials used to be commercial goods.
Embodiment
1 materials and methods
1.1 material cultures
Material to be tested is in the new land of non-Insect Resistant Cotton 61 and the new cotton 99 of soil of transgenic Bt cottonB, seed is respectively by Xinjiang Qian Hai
Zhong Ye Co., Ltds and institute of Chinese agriculture section Cotton Research Institute are provided.Experiment is carried out in illumination cultivation room, intensity of illumination
For 450 μm of ol m-2s-1, a length of 14h/10h during illumination/dark, temperature is (30 ± 2) DEG C/(20 ± 2) DEG C.Seed is with 9%
Dioxygen water sterilization 30min, deionized water presoaking and germinating is uniformly sowed at deionized water punching up to showing money or valuables one carries unintentionally after clear water rinsing for several times
Washed sand bed.Seedling is transferred in the plastics square box that volume is 32cm × 26cm × 32cm after 4d (6 plants of childrens is moved into per box
Seedling), to be cultivated with the Hoagland nutrient solutions (NS) of 1,1/2,1/4 times of improvement respectively, 24h ventilations are changed per 4d and once sought
Nutrient solution.The Hoagland nutrient solution prescriptions of improvement are：2.5mM Ca(NO3)2,1mM MgSO4,0.5mM(NH4)H2PO4,0.1mM
Fe-Na-EDTA,0.2μM CuSO4,1μM ZnSO4,20μM H3BO3,5pM(NH4)6Mo7O24With 1 μM of MnSO4.If abundance is supplied
Potassium 2.5mmol L-1With low potassium stress 0.03mmol L-12 process, contrast arrangement is often processed and repeats 6 boxes.
1.2 assay method
It is sampled at 4 leaf phase, with EPSON scanners (Seiko Epson Corp., Tokyo, Japan) root is scanned
Be form, with root system image analysis software Win Rhizo (version 4.0b, Regent Instruments Inc.,
Quebec, Canada 2000) analysis Root Parameters.By seedling after 105 DEG C of 30min that complete, dry at 80 DEG C until permanent
Weight；Pulverize and sieve after drying, use 1mol L-1HCl extracts 5h, vibrates 30min, filters, and uses atomic absorption spectrophotometer
(SpectAA-50/55, Varian, Australia) determines K+、Na+、Ca2+、Mg2+Concentration.Ion utilization index (Tian Xiaoli
Deng 2008) being tried to achieve using following equation：Ion utilization index (g2/ mg)=dry matter/ion concentration.
1.3 data statistics
All experiments are at least independently repeated 3 times, and each result trend is consistent, take and wherein representative are once united
Meter analysis.Using SPSS 16.0 (SPSS Inc.Chicago, USA) processing data, difference is carried out with Duncan ' s Multiple range tests
Significance test (P<0.05).
2 results and analysis
Different treatment conditions affect the dry matter accumulation of cotton variety seedling to see Fig. 1.Under the conditions of abundance is for potassium, two product
The dry matter accumulation planted is in downward trend with the reduction of nutrient solution concentration, is significantly reduced under the conditions of wherein 1/4NS dry
Thing weight.Compared with sufficient supplying under the conditions of potassium, low potassium condition inhibits the growth of seedling, wherein new cotton 99BWith in new land 61 it is dry
Photosynthetic matter accumulation under potassium sufficiency respectively than for reducing 64.2% and 23.5%.Under the conditions of low potassium, with nutrient solution concentration
Reduction, new cotton 99BDry matter accumulation dramatically increase；And 61 dry matter accumulation is in first increasing becoming of reducing afterwards in new land
Gesture, wherein the dry matter accumulation highest under the conditions of 1/2NS.
Different treatment conditions affect the Root morphology of different materials to be shown in Table 1.Under the conditions of abundance is for potassium, the root of two kinds
Long, root surface area and radical are significantly reduced as the reduction of nutrient solution concentration is in downward trend under the conditions of wherein 1/4NS
This several indexs.Compared with sufficient supplying under the conditions of potassium, the indices of root system are significantly reduced under the conditions of low potassium, especially new cotton
99BSuppressed degree is bigger, and such as root length, root surface area and radical inhibits respectively 79.4%, 89.6% and 90.1%.In low potassium
Under the conditions of, with the reduction of nutrient solution concentration, new cotton 99BThe indices of root system are dramatically increased, and in new land 61 root system shape
State is relatively stable, and nutrient solution concentration change is not made significant difference.
Impact of the different condition of table 1 to cotton seedling Root morphology
Note：The different letters of same index are represented in p<Significant difference in 0.05 level
Under the conditions of abundance is for potassium, the condition of culture of 1/4NS significantly reduces K+、Na+And Mg2+Accumulation, and nutrition
Liquid concentration is to Ca2+That what is accumulated does not make significant difference (Fig. 2).Low potassium condition reduces K+And Ca2+Accumulation, supply potassium bar than sufficient respectively
94.1% and 58.3% is reduced under part, Na is increased+Accumulation, be averagely sufficient 2.6 times under the conditions of potassium, to Mg2+'s
Difference between accumulation kind.Under the conditions of low potassium in different nutrient solutions, the accumulation of 61 four kinds of dominant cations in new land
It is above new cotton 99B, and new cotton 99BMaximum cation cumulant be in 1/4NS conditions, and in new land 61 in first increasing
The trend for reducing afterwards, wherein the cation accumulation highest under the conditions of 1/2NS.
Under for potassium sufficiency, as the reduction of nutrient solution concentration significantly reduces Ca2+Utilization index, to K+、
Na+And Mg2+Utilization index do not make significant difference (Fig. 3).Low potassium condition increased K+Utilization index, be sufficient under the conditions of potassium
5.2 times, reduce Na+、Ca2+、Mg2+Utilization index, respectively for potassium abundance under 11.6%, 72.5% and 19.6%.
Under the conditions of low potassium, with the reduction of nutrient solution dilute concentration, the utilization index of four kinds of cations presents the trend of increase, 1NS
The cation utilization index of condition of culture is substantially less than other two process, and the utilization of 1/2NS and 1/4NS condition of culture refers to
Number compares, and certain otherness is shown between kind, and 61 maintain the K of maximum such as in the new land of condition of culture of 1/2NS+With
Ca2+Utilization index, and new cotton 99BIt is then the condition of culture in 1/4NS.
As can be seen that under for potassium adequate level, the optimum nutrient solution concentration of cotton seedling is 1NS, such as higher is dry
Photosynthetic matter accumulation, preferable Root morphology etc., and under the conditions of low potassium, higher nutrient solution concentration can suppress on the contrary seedling and root system
Growth.In different growing environments, plant to the concentration and ratio of mineral element demand different, in nutrient solution nutrient
Under conditions of sufficient, environment is suitable, it is possible to increase the concentration of ion, on the contrary then preferably appropriate reduction concentration.This test result indicate that, 1/
2NS conditions can be used as rational water planting condition of culture, i.e., can be used as facing of not affecting it to grow under for potassium sufficiency
Dividing value, can also be used as the concentration of suitable growth under the conditions of low potassium.
Although above with a general description of the specific embodiments the present invention is described in detail,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, without departing from theon the basis of the spirit of the present invention these modifications or improvements, belong to the scope of protection of present invention.
Bibliography
1. Chen Yong is magnificent, Wu Xiaofu, Zhang Donglin, Chen Liangming, Chen Mingli, thunder and lightning, Zhang Xiuxiang, and gold is beautiful. and Different Nutrition liquid is dense
Impact of the degree with formula to water cultivation of ornamental plant. Sino-South African Forestry University of Science and Technology's journal, 2007,27 (6):34-37
2. fourth is elegant, the little support of Wu, Liu Minna, Wang Junjun, Lin Xianyong. different nutrient formula to mist training romaine lettuce biomass and
The impact of nutritional quality. journal of Zhejiang university (agricultural and life science version), 2012,38 (2):175-184
3. model wishes peak, Wang Hanxia, Tian Xiaoli. impact and optimal application quantity research of the potash fertilizer to output of cotton. Cotton Science,
2006,18(3):175-179.
4. Pan Jing is refined, yellow lead maple, Wang Shiping, Jia Zhikuan. and nutrient solution concentration is to Muskmelon Seedlings growth and the shadow of photosynthesis characteristics
Ring. plant nutrient and fertilizer journal 2005,11 (2):254-258
5. Peng Long, former to protect loyal, Zhou Huan. and potassium is to Developmental of Cotton physiological property and the influence research of yield and quality. in
State's agronomy circular, 2011,27 (12):227-231
6. Sun Hong men of virtue and ability, Yang Fan, Tao Hong, You Jie, Lin Shan. the shadow that forms of nitrogen and nutrient solution concentration grow to twocolor sealavander herb
Ring. China Agricultural University's journal, 2008,13 (1):36-41
7. Tian Xiaoli, Wang Gangwei, Zhu Rui, Yang Peizhu, Duan Liusheng, Li Zhaohu. cotton genotype tolarant to K deficiency type screening conditions and
The research of index. Acta Agronomica Sinica, 2008,34 (8):1435-1443
8.De Rijck G and Schrevens E.Application of mixture-theory for the
optimization of the composition of the nutrient solution.Acta Hort,1995,401:
283-291
9.Nukaya A,Goto K,Jang H,Kano A,Ohkawa K.Effect of K/Ca ratio in the
nutrient solution on incidence of blossom-end rot and gold specks of tomato
fruit grown in rock wool.Acta Hort,1995,396:123-130
10.Shima K,Kageyama Y,Konishi K.Effect of phosphorus application
rates along a nitrogen application curve on growth and cut flower quality in
chrysanthemum in NFT system(in Japanese with English summary).J Japan Soc
Hort Sci,1996,65(2):355-361
11.Wang N,Hua HB,Eneji E,Li ZH,Duan LS,Tian XL.Genotypic variations
in photosynthetic and physiological adjustment to potassium deficiency in
cotton(Gossypium hirsutum L.).J Photoch Photobio B,2012,110:1-8
12.Willumsen J,Petersen KK,Kaack K.Yield and blossom-end rot of
tomato as affected by salinity and cation activity ratios in the root zone.J
Hort Sci,1996,71(1):81-98
13.Zhang ZY,Tian XL,Duan LS Wang BM,He ZP,Li ZH.Differential
responses of conventional and Bt-transgenic cotton(Gossypium hirsutum L.)to
potassium deficiency.J Plant Nutr,2007,30:659-671。
Claims (9)
1. low-kalium resistant cotton seedling nutrient solution, it is characterised in that the formula of the nutrient solution is：1.25mM Ca(NO3)2,0.5mM
MgSO4,0.25mM(NH4)H2PO4,0.05mM Fe-Na-EDTA,0.1μM CuSO4,0.5μM ZnSO4,10μM H3BO3,
2.5pM(NH4)6Mo7O24With 0.5 μM of MnSO4。
2. application of the nutrient solution described in claim 1 in cotton seedling water planting culture.
3. application of the nutrient solution described in claim 1 in the screening of low-kalium resistant cotton variety.
4. application according to claim 3, it is characterised in that by cotton seedling with containing described in different potassium concentrations
Solution culture method for a period of time after, according to the Root morphology of seedling, and by determine seedling dry matter, Ca2+、K+、Na+And Mg2 +Level and utilization rate are screening low-kalium resistant cotton variety.
5. application according to claim 4, it is characterised in that cotton seeds are sterile-processed, after being rinsed with water, in water
Then presoaking and germinating is seeded in sand bed up to showing money or valuables one carries unintentionally, and seedling is transferred to into the battalion containing different potassium concentrations after 4d
Dry matter, ion concentration and the Ca that seedling is measured by sampling to 4 leaf phases is cultivated in nutrient solution culture2+、K+、Na+And Mg2+Utilization
Rate.
6. application according to claim 5, it is characterised in that comprise the following steps：
1) cotton seeds rinse for several times deionized water presoaking and germinating afterwards until dew with 9% dioxygen water sterilization 30min with water
In vain, it is seeded in the flushed sand bed of deionized water；Seedling is transferred to into the plastics that volume is 32cm × 26cm × 32cm after 4d
Ventilation culture is carried out in box, the nutrient solution containing different potassium concentrations is held in plastic casing, per box 6 plants of seedling are put into,
One time of nutrition liquid is changed per 4d；
2) cultivate to during 4 leaf phase and sample, with EPSON scanner scanning Root morphologies, with root system image analysis software Win Rhizo
Analysis Root Parameters；
3) by seedling after 105 DEG C of 30min that complete, dry to constant weight at 80 DEG C；Pulverize and sieve after drying, use 1mol L-1HCl soaks
5h is carried, 30min is vibrated, is filtered, with atomic absorption spectrophotometer K is determined+、Na+、Ca2+And Mg2+Concentration；
4) ionic availability is calculated using following equation：Ionic availability=dry matter/ion concentration；
5) according to step 2) -4) result, filter out low-kalium resistant cotton variety.
7. application according to claim 6, it is characterised in that step 1) in the condition of culture of seedling be：Intensity of illumination 450
μmol m-2s-1, a length of 14h/10h during illumination/dark, cultivation temperature during illumination is 30 ± 2 DEG C, cultivation temperature when dark
For 20 ± 2 DEG C.
8. the application according to any one of claim 3-7, it is characterised in that the concentration range of potassium ion is 0.03-
2.50mM。
9. the application according to any one of claim 3-8, it is characterised in that the low-kalium resistant cotton variety is included in new land
61st, distant cotton 18, CCRI 4, Ji cotton 66.
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| CN113804632A (en) * | 2021-09-08 | 2021-12-17 | 河南科技学院 | Identification method for genotype of cotton in efficient potassium utilization |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351601A (en) * | 2011-07-18 | 2012-02-15 | 湖北仙隆化工股份有限公司 | Cotton seedling culturing pesticide fertilizer composition |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
| CN105647537A (en) * | 2016-01-19 | 2016-06-08 | 山东棉花研究中心 | Soil conditioner applicable to saline and alkaline dryland sandy soil cotton field |
| CN105675856A (en) * | 2016-02-19 | 2016-06-15 | 中国农业科学院棉花研究所 | Method for measuring potassium ion absorption kinetics of cotton |
-
2016
- 2016-11-09 CN CN201610986192.7A patent/CN106613826A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351601A (en) * | 2011-07-18 | 2012-02-15 | 湖北仙隆化工股份有限公司 | Cotton seedling culturing pesticide fertilizer composition |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
| CN105647537A (en) * | 2016-01-19 | 2016-06-08 | 山东棉花研究中心 | Soil conditioner applicable to saline and alkaline dryland sandy soil cotton field |
| CN105675856A (en) * | 2016-02-19 | 2016-06-15 | 中国农业科学院棉花研究所 | Method for measuring potassium ion absorption kinetics of cotton |
Non-Patent Citations (3)
| Title |
|---|
| 华含白等: "辽棉18 与新棉99B 苗期耐低钾能力的差异及其机制", 《作物学报》 * |
| 王春霞等: "NH +4 对不同基因型棉花幼苗K+吸收和利用的影响", 《植物营养与肥料学报》 * |
| 郝艳淑: "不同棉花基因型钾效率特征及其根系形态的差异", 《作物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113804632A (en) * | 2021-09-08 | 2021-12-17 | 河南科技学院 | Identification method for genotype of cotton in efficient potassium utilization |
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