CN105675856A - Method for measuring potassium ion absorption kinetics of cotton - Google Patents
Method for measuring potassium ion absorption kinetics of cotton Download PDFInfo
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Abstract
The invention relates to the technical field of agriculture, in particular to a method for measuring potassium ion absorption kinetics of cotton. The method specifically comprises the steps that a cotton seedling is fixed in depletion liquid, and during depletion treatment of 0-1 h, a depletion liquid sample is taken once every 5-10 min; during depletion treatment of 1-h, the depletion liquid sample is taken once every 30-60 min; during depletion treatment of 10-30h, the depletion liquid sample is taken once every 2-4 h, and the concentrations of potassium ions in the depletion liquid samples are measured respectively. Related parameters in the experiment process are optimized, especially the sampling time interval is optimized, and a drawn absorption curve can reflect the potassium ion absorption law of the cotton more precisely. The method is easy to operate and high in precision, the absorption kinetics curve is smooth, and a theoretical basis is provided for accurate screening of the efficient gene type cotton and reasonable application of potash fertilizer.
Description
Technical field
The invention belongs to agricultural technology field, particularly relate to a kind of mensuration dynamic (dynamical) method of Cotton Gossypii Potassium Absorption.
Background technology
Potassium is one of three elements of crop alimentary, compared with soil validity nitrogen, phosphorus, effectiveness potassium content relative abundance in China's agricultural soil, originally potassium deficiency problem is not bery prominent, but the development along with agricultural production, crop yield improves, and the nutrient amount of taking away increases, and the nutrient being returned in soil falls sharply, tradition farm manure and plant ash amount of application reduce, nitrogen, phosphatization fertilizer use increase, cause nutrient to lack of proper care, and potassium deficiency in various degree occur. Alleviate the approach of China's potassium source shortage problem mainly by the following two kinds: rely on raising soil K content to obtain high crop yield on the one hand; Breeding high-efficiency kind makes plant adapt to environment on the other hand. People are found by long-term research, the different cultivars of Different Crop or same crop yield under same fertility level differs greatly, this species diversity is exactly the different genotype concrete manifestation to environmental adaptation sex differernce, also provides enlightenment and opportunity for the exploitation of superior genotypes simultaneously. Fe absorption kinetics is started in early 1950s and grows up, and Michaelis (MichaelisMenten) theory and equation thereof are explained the plant a kind of theory to the dynamic process that medium intermediate ion absorbs, the parameter I in the equationmaxAnd KmThe feature of plant absorption nutrient can be described quantitatively, thus provide the index of quantization for Nutrient Absorption feature between control variety, can be used to evaluate resistance to lean ability between kind, therefore suffer from paying attention to.
Fe absorption kinetics is used for the plant research to ionic absorption, quantizating index is provided for Nutrient Absorption feature between control variety, but adopt in vitro root to carry out the absorption test of short-term at the solution of different concentration on conventional research method, although this method also can obtain the result of reference value, but there is many weak points, absorption characteristic such as excised root Yu whole plant be differentiated, measure time with many Plant samples etc., certainly exist bigger sampling difference. In order to overcome these defects, complete plant plant is carried out the absorption test of long period by ClaassenandBarber (1974), propose the depletion method of Fe absorption kinetics research, by the ion concentration in results of regular determination solution, obtain the curvilinear equation of concentration time to time change, obtain snubber circui through mathematical calculation.But substantial amounts of it have been experienced that: kinetic parameter non-constant parameter, for set nutrient ion, they affected by many factors as floristics and kind, seedling age, the supply situation of nutrient, PH (GlassandSiddiqi, 1984; Schenk, 1996; Nissenetal.1974) etc.
For Cotton Gossypii, the method in prior art, its Potassium Absorption kinetic determination adopted is to use for reference the ion depletion method of little the crop such as Semen Tritici aestivi, Oryza sativa L., there is the defects such as crop plant type not easily operates relatively greatly, absorption curve fluctuation is bigger, it is therefore proposed that a kind of improvement dynamic (dynamical) method of Cotton Gossypii Potassium Absorption is necessary.
Summary of the invention
It is an object of the invention to not easily measure greatly for cotton plants, and the coarse defect such as not accurate of existing assay method, a kind of mensuration dynamic (dynamical) method of Cotton Gossypii Potassium Absorption of improvement is provided, related experiment parameter in mensuration process is adjusted by the method, establishes the experiment condition forming dissimilar absorption curve.
One of technical scheme is: a kind of mensuration dynamic (dynamical) method of Cotton Gossypii Potassium Absorption, and described method is: the 0-1h, interval 5-10min that process in exhaustion take once exhaustion liquid sample; The 1-10h, interval 30-60min that exhaustion processes takes once exhaustion liquid sample; The 10-30h, interval 2-4h that exhaustion processes takes once exhaustion liquid sample, measures the potassium concentration exhausted in liquid sample respectively.
Described being fixed as adopts sponge to be wrapped up cotton seedling rhizome junction, is fixed in the container filling hungry liquid by cotton seedling by sponge.
This area routine techniques means can be adopted to measure and to exhaust potassium concentration in liquid, present invention preferably employs atomic spectrophotometer measurement.
Its sample point of existing sampling method is longer for interval time, and the depletion experimental persistent period is shorter, there is the curve drawn out unsmooth, and only one of which absorption equilibrium, and absorption curve can not really reflect that Cotton Gossypii absorbs the defects such as dynamic process. Sample interval in depletion experimental process and sampling duration are optimized by the present invention, namely at depletion experimental incipient stage intensive sampling, being found surprisingly that for a leaf phase and two leaf stage cotton seedling, its Potassium Absorption kinetic curve also exists two absorption equilibriums.
Preferably, exhaust that in processing procedure, the interval of sampling is: 5,10,10,15,30,30,30,30,30,60,60,60,60,60,60,60,120,120,180,180,240,240min, adopt the Potassium Absorption kinetic curve that this kind of interval sampling is drawn out to smooth, it is possible to reflect the Potassium Absorption rule of Cotton Gossypii more accurately.
The method that exhaustion processes can adopt the technological means that this area is conventional, is fixed in exhaustion liquid by cotton seedling, and continuous sampling and measuring exhausts the potassium concentration of liquid. Wherein, described exhaustion liquid can adopt liquid commonly used in the art, and the present invention preferably exhausts that liquid is: 4-6mmolL-1MES、180-220μmolL-1Ca2SO4, pH=6.5-7.0,100-300 μm of olL-1KCl is 5mmolL more preferably-1MES、200μmolL-1Ca2SO4, pH=6.8,100-300 μm of olL-1KCl。
Exhaust that in liquid, potassium ion is provided by potassium chloride, its purpose is to the introducing avoiding other ion and experiment is had a negative impact (as adopted potassium sulfate, owing to exhausting that in liquid, itself is containing sulfate radical, sulfate radical in potassium sulfate can make the sulfate concentration in exhaustion liquid increase, as adopted potassium nitrate, owing to the N element in potassium nitrate is one of a great number of elements required for plant, it exists, and to be likely to affect be the absorption of a great number of elements potassium required for plant equally), affect objectivity and the accuracy of measurement result.
In order to increase dissolved oxygen amount and reach the purpose of stirring in prior art, when measuring the absorption dynamics of plant, it is usually and is placed on shaking table by experimental system to carry out, this kind of mode is suitable for measuring the absorption dynamics of little crop such as such as Semen Tritici aestivi, Oryza sativa L. etc., for Cotton Gossypii, owing to its plant is relatively big, it is placed on shaking table and easily occurs that cane fractures or branch and leaf sustain damage, sample the problems such as inconvenience, finally affect the precision of measurement result. In order to overcome drawbacks described above, Cotton Gossypii is fixed in exhaustion liquid by the present invention, by exhausting that passing into air agitation in liquid exhausts that liquid is to get to the purpose increasing dissolved oxygen amount and stirring.
Preferably, in exhausting processing procedure, continue to pass into air in exhaustion liquid with small inflatable pump, passing into air and help lend some impetus to the aerobic respiration of root, the absorption for potassium element provides more energy, has also functioned to stirring action simultaneously, make exhaustion liquid more uniform, it is ensured that the precision of measurement result. The power of small inflatable pump is advisable with 5-10W, and power is higher easily to be made exhaustion liquid rising too fast or splashes, and power is relatively low does not have due effect.
In order to ensure precision and the objectivity of measurement result, before exhaustion processes, make K too much in plant body as far as possible+Ion is discharged, and eliminates internal K+Interference, it is preferable that before exhaustion processes, also including the step that cotton seedling carries out Nature enemy, the operation of described Nature enemy, particularly as follows: cotton seedling is fixed on Nature enemy 20-30h in hungry liquid, changes once hungry liquid at interval of 8-10h. The preferred operations of described Nature enemy is: cotton seedling is fixed on Nature enemy 24h in hungry liquid, changes once hungry liquid at interval of 8h.
Similarly, in Nature enemy process, continue to pass into air in hungry liquid.
Described hungry liquid can adopt liquid commonly used in the art, present invention preferably employs following hungry liquid: 4-6mmolL-1MES, 180-220 μm of olL-1Ca2SO4, pH=6.5-7.0, it is preferred that described hungry liquid is: 5mmolL-1MES, 200 μm of olL-1Ca2SO4, pH=6.8. In order to avoid introducing potassium ion in hungry liquid, when the hungry liquid of preparation, Tris and the HCl of low concentration is adopted to regulate pH value.
In order to carry out effective hunger in prior art, the Nature enemy time is usually 36-48h, but for Cotton Gossypii, owing to its plant is relatively big, needs the Nature enemy of longer time in theory; But, the present invention changes hungry liquid during Nature enemy, accelerates K+Outer row's effect of ion, the K when Nature enemy terminates, in the hungry liquid of replacing+Concentration is at 5 μm of oll-1Hereinafter, adopt the Nature enemy means of the present invention, just effectively cotton seedling can be carried out Nature enemy at 20-30h. Simultaneously in Nature enemy process, add the manipulation continuing to pass into air stirring solution in hungry liquid, it is possible to reduce the K around root system rapidly+Ion concentration, is also beneficial to improve K+Outer row's effect of ion.
The cotton seedling that the present invention relates to can adopt method well known in the art to cultivate, cultivate preferably by following method and obtain: after cotton seeds is sterilized, soak seed overnight with deionized water, select the seed that shows money or valuables one carries unintentionally of the same size, uniformly it is seeded on the bed that deionized water is washed, four layers of gauze of upper cover, then cover one layer of sand, send out Seedling and after 3-5 days, seedling is moved in container, cultivate with following improvement Hoagland nutritional solution: 2～3mMCa (NO3)2, 0.5～1.5mMMgSO4, 0.2～0.8mM (NH4)H2PO4, 1 × 10-4～3 × 10-4mMCuSO4, 0.5 × 10-3～1.5 × 10-3mMZnSO4, 0.05～0.15mMFeNaEDTA, 1 × 10-23×10-2mMH3BO3, 4 × 10-6～5 × 10-6mM(NH4)6Mo7O24With 0.5 × 10-3～1.5 × 10-3mMMnSO4, 0.05mmolL-1～2.5mmolL-1K+, incubation is continually fed into air, within every 3-5 days, changes a culture fluid.
Preferably, described improvement Hoagland nutritional solution is: 2.5mMCa (NO3)2, 1mMMgSO4,0.5mM(NH4)H2PO4, 2 × 10-4mMCuSO4, 1 × 10-3mMZnSO4, 0.1mMFeNaEDTA, 2 × 10-2mMH3BO3, 5 × 10-6mM(NH4)6Mo7O24With 1 × 10-3mMMnSO4, 0.05mmolL-1～2.5mmolL-1K+。
The present invention also provides for a kind of preferred mensuration dynamic (dynamical) method of Cotton Gossypii Potassium Absorption, comprises the steps:
(1) cotton seedling is fixed in hunger liquid and carries out Nature enemy;
(2) the cotton seedling immigration exhaustion liquid after Nature enemy will carry out exhaustion process, exhaust and processing procedure continues in container, pass into air, take exhaustion liquid sample according to following interval, measure potassium concentration: exhaustion processes 0-1h, interval 5-10min and takes a sample; Exhaustion processes 1-10h, interval 30-60min and takes a sample; Exhaustion processes 10-30h, interval 2-4h and takes a sample, measures and exhausts potassium concentration in liquid;
(3) process the time for abscissa with exhaustion, exhaust that in liquid sample, potassium concentration is that vertical coordinate draws Potassium Absorption kinetic curve.
Meeting on the basis of this area general knowledge, above-mentioned each optimum condition, it is possible to be mutually combined, obtain each preferred embodiment of the present invention.
The all commercially available acquisition of reagent of the present invention and material.
The method of the invention has the advantage that:
(1) by the relevant parameter in experimentation is optimized, the perfect dynamic (dynamical) method of Cotton Gossypii Potassium Absorption; Wherein sample interval and sampling duration are optimized, reflect the Potassium Absorption rule of Cotton Gossypii more accurately;
(2) in experimentation with small inflatable pump substitute shaking table, overcome cane fracture or branch and leaf sustain damage, sample inconvenience etc. problem;
(3) in Nature enemy process, the measure changed hungry liquid with continue to pass into air in hungry liquid is adopted, it is possible to effectively cotton seedling is carried out Nature enemy in the short period of time;
(4) the method is simple to operate, and precision is high, the absorption dynamics curve smoothing of drafting, and the Rational Application for accurately screening and the potash fertilizer of high efficiency gene type Cotton Gossypii provides theoretical foundation.
Accompanying drawing explanation
Fig. 1 is the different absorption curves exhausting next leaf phase cotton seedling of liquid potassium concn condition.
The absorption curve of two leaf stage cotton seedling when Fig. 2 is different exhaustion liquid potassium concn.
Fig. 3 is the different absorption curves exhausting cotton seedling in tri-leaf period when liquid potassium concn.
The absorption curve of five leaf phase cotton seedlings when Fig. 4 is different exhaustion liquid potassium concn.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention. The cotton seedling related in embodiment is cultivated by the following method and is obtained:
Material to be tested is CCRI 35, the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute provide; Test carries out in illumination cultivation indoor, and intensity of illumination is 450 μm of olm-2s-1, light application time is 14h, and day temperature is 30 DEG C ± 2 DEG C, and nocturnal temperature is 20 DEG C ± 2 DEG C. Seed is sterilized 30min with the hydrogen peroxide of 9%, and clear water rinsing is soaked seed overnight with deionized water for several times afterwards, selects the seed that shows money or valuables one carries unintentionally of the same size, is uniformly seeded on the bed that deionized water is washed, four layers of gauze of upper cover, then cover Bao Sha mono-layer. Send out Seedling and after 4 days, seedling is transferred in the plastics square box that volume is 32cm × 26cm × 32cm, respectively with containing different potassium concn (low potassium stress 0.05mmolL-1With abundance for potassium 2.5mmolL-1) the Hoagland nutritional solution improved cultivates; Nutrient solution prescription is (mM): 2.5mMCa (NO3)2, 1mMMgSO4,0.5mM(NH4)H2PO4, 2 × 10-4mMCuSO4, 1 × 10-3mMZnSO4, 0.1mMFeNaEDTA, 2 × 10-2mMH3BO3, 5 × 10-6mM(NH4)6Mo7O24 and 1 × 10-3mM MnSO4.Every box moves into 6 strain seedling, the strain of each seed soaking 3. 24h ventilates, and every 4d changes one time of nutrition liquid; Contrast arrangement, often processes and repeats 6 boxes. Test repeats more than 4 times.
The Potassium Absorption kinetic determination of embodiment 1 one leaf phase cotton seedling
Nature enemy: select in abundance for potassium 2.5mmolL-1Consistent cotton seedling 6 strain of the leaf phase obtained, representative growth (between group, weight differs less than 2g) is cultivated under (high potassium) condition, 3 strains are as a repetition (3 repetitions), being rinsed well by seedlings root deionized water, moisture is dried in absorbent paper. Taking triangular flask, each triangular flask puts 600mL hunger liquid (5mmolL-1MES、200μmolL-1Ca2SO4, pH=6.8), by seedling rhizome junction with sponge wrapping together, it is fixed in triangular flask to carry out the Nature enemy (every 8h changes once hungry liquid) of 24h; Should be specifically noted that in the process and avoid making root system sustain damage, in triangular flask, keep aeration status simultaneously.
Exhaustion processes: after Nature enemy 24h, is taken out by cotton seedling and blot root tissue water status with absorbent paper from hungry liquid, and (with hungry liquid preparation, potassium ion is provided by potassium chloride, and concentration is 106 μm of olL respectively to put into the exhaustion liquid that fills 600ml difference potassium concn-1,163μmolL-1,210μmolL-1) triangular flask in, and be fixed in triangular flask and carry out depletion experimental (avoiding root system to sustain damage); Timing simultaneously, is placed in the 1mL centrifuge tube of good number of mark respectively at different time points sucking-off 0.5mL from triangular flask; The interval of sampling is set to: 5,10,10,15,30,30,30,30,30,60,60,60,60,60,60,60,120,120,1 80,180,240,240min. In order to keep the vigor of cotton seedling root system and make exhaustion liquid more uniform, aeration status persistently should be kept in triangular flask; Meanwhile, for making up owing to evaporating lost moisture, hungry liquid supplement and make it maintain fixing exhaustion liquid to amass. After depletion experimental terminates, measured by atomic absorption spectrophotometer (SpectAA-50/55, Varian, Australia) and exhaust potassium concn in liquid.
Drawing curve: with the time of exhausting for abscissa, potassium concentration is that vertical coordinate draws curve, and result is as shown in Figure 1.
The Potassium Absorption kinetic determination of embodiment 2 two leaf stage cotton seedling
The operational approach of the present embodiment is with embodiment 2, and it is different with seedling age that it differs only in potassium concentration in exhaustion liquid, and the present embodiment exhausts that in liquid, potassium concentration is respectively as follows: 162 μm of olL-1, 204 μm of olL-1, 252 μm of olL-1, seedling age is two leaf stage seedling, and the absorption dynamics curve that drafting obtains is as shown in Figure 2.
The Potassium Absorption kinetic determination of embodiment 3 cotton seedling in tri-leaf period
The operational approach of the present embodiment is with embodiment 2, and it is different that it differs only in experiment material used; Material used by the present embodiment is: at low potassium stress 0.05mmolL-1(low potassium) and abundance are for potassium 2.5mmolL-1The cotton seedling in tri-leaf period is cultivated under (high potassium) condition; The absorption dynamics curve that drafting obtains is as shown in Figure 3.
The Potassium Absorption kinetic determination of embodiment 4 five leaf phase cotton seedling
The operational approach of the present embodiment is with embodiment 3, and its material differing only in experiment used is five leaf phase seedling, and the absorption dynamics curve that drafting obtains is as shown in Figure 4.
By Fig. 1 and Fig. 2 it can be seen that a leaf phase seedling all shows two absorption processes at absorption curve when exhausting initial concentration three kinds different, absorbing phenomenon stage by stage occurs significantly; Wherein when exhausting that liquid concentration reduces about 25 μm of ol, three absorption curves of a leaf phase seedling reach balance;Next absorption process is shown again after absorption equilibrium persistently about 420min. Two leaf stage seedling also shows similar situation at absorption dynamics curve when exhausting initial concentration three kinds different; But after two leaf stage seedling absorbs more potassium ion, absorption curve just reaches balance, and absorption equilibrium duration is also compared with a leaf phase seedling short (240min).
By Fig. 3 and Fig. 4 it can be seen that under 252 μm of ol initial exhaustion liquid concentration conditions, the different tri-leaf periods of potassium CMC model and the absorption curve of five leaf phase seedling are an absorption process, it does not have absorbing phenomenon stage by stage occur significantly; The seedling absorption rate that low potassium is cultivated is more than the seedling of high potassium CMC model, and along with the growth of seedling age, different potassium concns grow seedlings between absorption curve difference be gradually lowered.
Summary, the absorption of a leaf phase and two leaf stage seedling all shows obvious absorption process stage by stage and unrelated with exhausting liquid concentration, it may be possible to owing to cotton seedling is less, the demand for potassium is relatively low caused; Tri-leaf period and five leaf phase seedling are higher to the demand of potassium, and phenomenon stage by stage does not occur significantly in absorption curve.
Although, above use generality explanation, detailed description of the invention and test, the present invention is described in detail, but on basis of the present invention, it is possible to it is made some modifications or improvements, and this will be apparent to those skilled in the art. Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. one kind measures the dynamic (dynamical) method of Cotton Gossypii Potassium Absorption, it is characterised in that be fixed on by cotton seedling in exhaustion liquid, and the 0-1h, interval 5-10min that process in exhaustion take once exhaustion liquid sample; The 1-10h, interval 30-60min that exhaustion processes takes once exhaustion liquid sample; The 10-30h, interval 2-4h that exhaustion processes takes once exhaustion liquid sample, measures the potassium concentration exhausted in liquid sample respectively.
2. method according to claim 1, it is characterised in that: exhaust that in processing procedure, the interval of sampling is: 5,10,10,15,30,30,30,30,30,60,60,60,60,60,60,60,120,120,180,180,240,240min.
3. method according to claim 1 and 2, it is characterised in that: described exhaustion liquid is: 4-6mmolL-1MES, 180-220 μm of olL-1Ca2SO4, 100-300 μm of olL-1KCl, pH=6.5-7.0;
Preferably, described exhaustion liquid is 5mmolL-1MES, 200 μm of olL-1Ca2SO4, 100-300 μm of olL-1KCl, pH=6.8.
4. according to the arbitrary described method of claim 1-3, it is characterised in that: exhaust in processing procedure, continue to pass into air in exhaustion liquid.
5. method according to claim 4, it is characterized in that: before exhaustion processes, also include the step that cotton seedling is carried out Nature enemy, the operation of described Nature enemy, particularly as follows: cotton seedling is fixed on Nature enemy 20-30h in hungry liquid, changes once hungry liquid at interval of 8-10h;
Preferably, cotton seedling is fixed on Nature enemy 24h in hungry liquid, changes once hungry liquid at interval of 8h.
6. method according to claim 5, it is characterised in that: described hungry liquid is: 4-6mmolL-1MES, 180-220 μm of olL-1Ca2SO4, pH=6.5-7.0;
Preferably, described hungry liquid is: 5mmolL-1MES, 200 μm of olL-1Ca2SO4, pH=6.8.
7. the method according to claim 5 or 6, it is characterised in that: in Nature enemy process, continue to pass into air in hungry liquid.
8. according to the arbitrary described method of claim 1-7, it is characterized in that: described cotton seedling is cultivated by the following method and obtained: after cotton seeds is sterilized, soak seed overnight with deionized water, select the seed that shows money or valuables one carries unintentionally of the same size, uniformly it is seeded on the bed that deionized water is washed, upper cover gauze, then cover sand, send out Seedling and after 3-5 days, seedling is moved in container, cultivate with following improvement Hoagland nutritional solution: 2～3mMCa (NO3)2, 0.5～1.5mMMgSO4, 0.2～0.8mM (NH4)H2PO4, 1 × 10-4～3 × 10-4mMCuSO4, 0.5 × 10-3～1.5 × 10-3mMZnSO4, 0.05～0.15mMFeNaEDTA, 1 × 10-23×10-2mMH3BO3, 4 × 10-6～5 × 10-6mM(NH4)6Mo7O24With 0.5 × 10-3～1.5 × 10-3mMMnSO4, 0.05mmolL-1～2.5mmolL-1K+, incubation is continually fed into air, within every 3-5 days, changes a culture fluid.
9. method according to claim 8, it is characterised in that: described improvement Hoagland nutritional solution is: 2.5mMCa (NO3)2, 1mMMgSO4,0.5mM(NH4)H2PO4, 2 × 10-4mMCuSO4, 1 × 10-3mMZnSO4, 0.1mMFeNaEDTA, 2 × 10-2mMH3BO3, 5 × 10-6mM(NH4)6Mo7O24With 1 × 10-3mMMnSO4, 0.05mmolL-1～2.5mmolL-1K+。
10. according to the arbitrary described method of claim 1-9, it is characterised in that described method comprises the steps:
(1) cotton seedling is fixed in hunger liquid and carries out Nature enemy;
(2) the cotton seedling immigration exhaustion liquid after Nature enemy will carry out exhaustion process, exhaust and processing procedure continues in container, pass into air, take exhaustion liquid sample according to following interval, measure potassium concentration: exhaustion processes 0-1h, interval 5-10min and takes a sample; Exhaustion processes 1-10h, interval 30-60min and takes a sample; Exhaustion processes 10-30h, interval 2-4h and takes a sample, measures and exhausts potassium concentration in liquid;
(3) process the time for abscissa with exhaustion, exhaust that in liquid sample, potassium concentration is that vertical coordinate draws Potassium Absorption kinetic curve.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613826A (en) * | 2016-11-09 | 2017-05-10 | 中国农业科学院棉花研究所 | Low-potassium-tolerant cotton seedling nutrient solution and application thereof |
| CN107219174A (en) * | 2017-05-09 | 2017-09-29 | 中国农业科学院棉花研究所 | One authentication method for growing cotton low-kalium resistant germplasm |
| CN108776179A (en) * | 2018-06-11 | 2018-11-09 | 江苏省中国科学院植物研究所 | A kind of evaluation grass-leaved sweetflag absorption dynamic (dynamical) method of nitrate ion |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554240A (en) * | 2013-11-01 | 2014-02-05 | 中国农业大学 | Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof |
-
2016
- 2016-02-19 CN CN201610095013.0A patent/CN105675856B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554240A (en) * | 2013-11-01 | 2014-02-05 | 中国农业大学 | Protein GhKT2 related to potassium ion absorption capacity of plant as well as coding gene and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘静雯等: "氮饥饿细基江蓠繁枝变型和孔石莼氨氮的吸收动力学特征", 《海洋学报》 * |
| 张志勇等: "棉花钾吸收动力学的初步研究和应用", 《棉花学报》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106613826A (en) * | 2016-11-09 | 2017-05-10 | 中国农业科学院棉花研究所 | Low-potassium-tolerant cotton seedling nutrient solution and application thereof |
| CN107219174A (en) * | 2017-05-09 | 2017-09-29 | 中国农业科学院棉花研究所 | One authentication method for growing cotton low-kalium resistant germplasm |
| CN108776179A (en) * | 2018-06-11 | 2018-11-09 | 江苏省中国科学院植物研究所 | A kind of evaluation grass-leaved sweetflag absorption dynamic (dynamical) method of nitrate ion |
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