CN106591116A - Reaction device capable of eliminating inhibiting effect of enzymolysis product on enzymatic activity - Google Patents
Reaction device capable of eliminating inhibiting effect of enzymolysis product on enzymatic activity Download PDFInfo
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- CN106591116A CN106591116A CN201611033617.9A CN201611033617A CN106591116A CN 106591116 A CN106591116 A CN 106591116A CN 201611033617 A CN201611033617 A CN 201611033617A CN 106591116 A CN106591116 A CN 106591116A
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- enzymolysis
- enzymatic hydrolysate
- enzyme activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
The invention belongs to the field of enzymolysis technologies, and in particular discloses a reaction device capable of eliminating an inhibiting effect of an enzymolysis product on enzymatic activity. The device comprises a substrate supplementing system, an enzymolysis reaction system, an enzymolysis adsorption system and a product recovery system, wherein the various systems are fixed by virtue of an iron stand and are connected by virtue of a pipeline system. By virtue of a dialysis membrane and a product adsorption device of an enzymolysis device reactor, the inhibiting effect of the enzymolysis product on the enzymatic activity can be eliminated; in combination with the substrate supplementing system, the product adsorption system, the product recovery system and a control system, automatic and continuous production can be achieved; and the material of the dialysis membrane can be selected as required, so that repeated utilization in the same device is achieved. With the application of the device, the inhibiting effect of the product from an enzymolysis production process on the enzymatic activity is effectively eliminated; meanwhile, the rapid, simple, efficient, continuous and automatic implementation of the enzymolysis process is achieved; and at the same time, a function of realizing the repeated utilization of enzymolysis reaction liquid is achieved. The reaction device has a great application prospect in the production process of preparing the product by virtue of an enzymolysis approach.
Description
Technical field
The invention belongs to enzymolysis process field, is specifically related to a kind of reaction dress of elimination enzymatic hydrolysate inhibitory enzyme activity
Put.
Background technology
Physical, chemical method and enzymatic isolation method are three kinds of major ways for preparing biological product.With Physical and chemical method phase
There is the advantages such as substrate is single-minded, product is homogeneous and reaction condition is gentle than, enzymatic isolation method, but in most of enzymolysis process,
Accumulating a certain amount of enzymatic hydrolysate can carry out feedback suppression to enzymatic activity, cause enzymolysis process to terminate.No matter is this bottleneck problem
Tangible laboratory angle still produces the scale that enzymolysis process is all limited in angle.For above-mentioned bottleneck problem, energy is developed
In time, efficiently, the difficult problem that enzymatic hydrolysate is urgent need to resolve to the method for the inhibitory action of enzymatic activity is accurately eliminated.
The content of the invention：
The invention aims to eliminate product is provided the feedback inhibition of enzymatic activity in enzymolysis process one
Plant device.The device has and effectively eliminate inhibitory action of the product to enzymatic activity, and with seriality, simple, efficiently, automatically
The advantages of change, recycling and product reclaim simple.
In order to solve above-mentioned technical problem, the technical solution used in the present invention is as follows：
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, including substrate replenishment system, enzyme digestion reaction system, enzyme
Desorption system, product recovery, the substrate replenishment system includes container, pipe-line system, the dynamical system of storage substrate；
The enzyme digestion reaction system includes inside and outside cutting ferrule, dialyzer, enzyme digestion reaction control system (stirring, temperature control and pH control)；It is described
Enzymatic hydrolysate adsorption system includes enzymatic hydrolysate specific adsorption post, pipe-line system, dynamical system, enzyme digestion reaction liquid and waste liquid
Valve；The enzymatic hydrolysate recovery system includes enzymatic hydrolysate specificity eluent, pipe-line system, dynamical system；The substrate is mended
Charging system, enzyme digestion reaction system, enzymolysis adsorption system, product recovery are fixed by iron stand, pipe-line system is connected.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the container of the storage substrate is the burning of different ranges
Cup.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the pipe-line system is latex tubing, and dynamical system is
Peristaltic pump, specification and the peristaltic pump of latex tubing match.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the inside and outside cutting ferrule is with dialyzer using detachable
Attachment structure.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the material of the dialyzer is molecular sieve.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the enzyme digestion reaction control system is band heating function
Magnetic stirring apparatuss, and the adjustment of pH is carried out by way of labor measurement pH, the function of possessing stirring, regulation and control pH and temperature,
Enzyme-to-substrate is promoted more fully to contact.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption column is Demountable.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption column is activated carbon.
The switch of a kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption system and recovery system leads to
Cross Valve controlling.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the valve is in the form of glass three-way.
Compared with prior art, the present invention has advantages below：
1. enzymatic hydrolysate can depart from reaction system by dialyzer, effectively eliminate its inhibitory action to enzymatic activity.
2. the material for making dialyzer can be selected according to specific experiment, a device multiple use is realized；
3. the continuous supplementation of substrate is realized by substrate replenishment system, shadow of the initial high concentration substrate concentration to reaction is eliminated
Ring, improve substrate utilization ratio；
4. product adsorption system is capable of achieving the enrichment of enzymatic hydrolysate, and product adsorption column can change different absorption according to experiment
Material, realizes the recycling of pillar；
5. product adsorption system is capable of achieving the reuse of flow fluid (going to dezymotize the reactant liquor with substrate)；
6. product recovery carries out desorption using product specificities eluent, realizes that the stepization of product one is collected, while
Exempt and change pillar.
Description of the drawings
Fig. 1 is the structural representation of the reaction unit that the present invention eliminates enzymatic hydrolysate inhibitory enzyme activity, wherein, 1. substrate is mended
Charging system, 2. enzyme digestion reaction system, 3. enzymolysis adsorption system, 4. product recovery, the 6. container for 5. storing substrate, pipeline system
System, 7. dynamical system, 8. dialyzer, 9. inside and outside cutting ferrule, 10. enzyme digestion reaction control system, 11. specific adsorption posts, 12. special
Property eluent, 13. valves, the direction of arrow be device in reactant liquor flow direction.
Specific embodiment：
Below in conjunction with the accompanying drawings the present invention is described in detail with specific embodiment.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, as shown in figure 1, including substrate replenishment system 1, enzymolysis
Response system 2, enzymolysis adsorption system 3, product recovery 4, the substrate replenishment system 1 includes the container 5 of storage substrate, pipe
Road system 6, dynamical system 7, are capable of achieving continuous, the controllable supplement of substrate；The enzyme digestion reaction system include inside and outside cutting ferrule 9,
Dialyzer 8, enzyme digestion reaction control system 10, inside and outside cutting ferrule is fixed in retracting device by three foot rests, can be according to reactant
The different materials for changing dialyzers；The enzymatic hydrolysate adsorption system 3 includes enzymatic hydrolysate specific adsorption post 11, pipeline system
System 6, dynamical system 7, enzyme digestion reaction liquid and waist valve, are capable of achieving the continuous absorption of product；The enzymatic hydrolysate recovery system 4 is wrapped
Enzymatic hydrolysate specificity eluent 12, pipe-line system 6, dynamical system 7 are included, the one-step method for realizing product is collected；The substrate is mended
Charging system 1, enzyme digestion reaction system 2, enzymolysis adsorption system 3, product recovery 4 are fixed by iron stand, the phase of pipe-line system 6
Even.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the container 5 of the storage substrate is different ranges
Beaker.
It is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, the pipe-line system 6 be latex tubing, dynamical system 7
For peristaltic pump, specification and the peristaltic pump of latex tubing match.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the inside and outside cutting ferrule 9 is with dialyzer 8 using detachable
Formula attachment structure.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the material of the dialyzer 8 is molecular sieve.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the enzyme digestion reaction control system 10 is band plus hot merit
The magnetic stirring apparatuss of energy, and the adjustment of pH is carried out by way of labor measurement pH, possess the work(of stirring, regulation and control pH and temperature
Can, promote enzyme-to-substrate more fully to contact.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption column 11 is Demountable.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption column 11 is activated carbon.
A kind of switch of the reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the adsorption system 3 and recovery system 4
Controlled by valve 13.
A kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, the valve 13 is in the form of glass three-way.
The experiment that carrageenase enzymatic isolation method prepares Carrageenan sugar is carried out using apparatus of the present invention：Continuously added with certain flow rate
Plus the substrate containing carrageenan (0.7g/100ml), dialyzer 8 is that (enzymatic hydrolysate is 400- more than 2000Da for interception
1700Da), reaction temperature is 45 DEG C, and pH is 7, and rotating speed is 150rpm, and reactant liquor is PBS buffer solution, and adsorption flow liquid is PBS
Buffer solution, adsorption column 11 adopts activated carbon, the controllable peristaltic velocity regulation and control product rate of adsorption to release as necessary by flow fluid
Put valve replacement flow fluid；Eluent 12 is 20-40% ethanol, and product dry powder is obtained using lyophilization after eluting.By upper
Reaction unit is stated, carrageenase enzymatic isolation method prepares the throughput rate of Carrageenan sugar and improves 300%.
The above-mentioned description to embodiment is the research worker for ease of enzymolysis process field and those of ordinary skill's use
It is bright.Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein
General Principle is applied in other embodiment without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment,
Those skilled in the art's announcement of the invention, the improvement made without departing from scope and modification all should be at these
Within bright protection domain.
Claims (10)
1. a kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity, it is characterised in that anti-including substrate replenishment system, enzymolysis
Answer system, enzymolysis adsorption system, product recovery, the substrate replenishment system include the storage container of substrate, pipe-line system,
Dynamical system；The enzyme digestion reaction system includes inside and outside cutting ferrule, dialyzer, enzyme digestion reaction control system；The enzymatic hydrolysate is inhaled
Attached system includes enzymatic hydrolysate specific adsorption post, pipe-line system, dynamical system, enzyme digestion reaction liquid and waist valve；The enzymolysis
Product recovery includes enzymatic hydrolysate specificity eluent, pipe-line system, dynamical system；The substrate replenishment system, enzymolysis
Response system, enzymolysis adsorption system, product recovery, pipe-line system are connected.
2. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
The container of storage substrate is the beaker of different ranges.
3. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
Pipe-line system is latex tubing, and dynamical system is peristaltic pump, and specification and the peristaltic pump of latex tubing match.
4. a kind of reaction unit for eliminating enzymatic hydrolysate inhibitory enzyme activity according to claim 1, it is characterised in that a kind of
The reaction unit of enzymatic hydrolysate inhibitory enzyme activity is eliminated, the inside and outside cutting ferrule adopts detachable connection structure with dialyzer.
5. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
The material of dialyzer is molecular sieve.
6. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
Enzyme digestion reaction control system is the magnetic stirring apparatuss with heating function, and the adjustment of pH is carried out by way of labor measurement pH,
Possesses the function of stirring, regulation and control pH and temperature.
7. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
Adsorption column is Demountable.
8. it is according to claim 7 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
Adsorption column is activated carbon.
9. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that it is described
The switch of adsorption system and recovery system passes through Valve controlling.
10. it is according to claim 1 it is a kind of eliminate enzymatic hydrolysate inhibitory enzyme activity reaction unit, it is characterised in that institute
Valve is stated in the form of glass three-way.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611033617.9A CN106591116A (en) | 2016-11-22 | 2016-11-22 | Reaction device capable of eliminating inhibiting effect of enzymolysis product on enzymatic activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611033617.9A CN106591116A (en) | 2016-11-22 | 2016-11-22 | Reaction device capable of eliminating inhibiting effect of enzymolysis product on enzymatic activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106591116A true CN106591116A (en) | 2017-04-26 |
Family
ID=58592658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611033617.9A Pending CN106591116A (en) | 2016-11-22 | 2016-11-22 | Reaction device capable of eliminating inhibiting effect of enzymolysis product on enzymatic activity |
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| CN (1) | CN106591116A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116515628A (en) * | 2023-07-03 | 2023-08-01 | 鹤岗萃元科技产业发展有限公司 | Preparation facilities of full enzymatic synthesis diglyceride |
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|---|---|---|---|---|
| US6107084A (en) * | 1995-10-19 | 2000-08-22 | Mitsuhiko Onda | Method for the preparation of an immobilized protein ultrathin film reactor and a method for a chemical reaction by using an immobilized protein ultrathin film reactor |
| CN1266906A (en) * | 2000-03-01 | 2000-09-20 | 刘树军 | Process and equipment for preparing low-molecular peptide from sodium caseinate as raw material |
| US20030012798A1 (en) * | 2000-01-12 | 2003-01-16 | Tetsuro Ikekawa | Physiologically active substance eem-s originating in mushroms, process for producing the same and drugs |
| CN1563405A (en) * | 2004-04-01 | 2005-01-12 | 广西大学 | Method for preparing peptide of soybean through continuous enzyme membrane reaction of soyabean protein |
| CN101724555A (en) * | 2009-12-18 | 2010-06-09 | 中国农业大学 | Whey protein antihypertensive peptide prepared by utilizing continuous enzyme membrane reactor and special device thereof |
| CN102453741A (en) * | 2010-10-18 | 2012-05-16 | 华中农业大学 | Method for preparing high-activity corn antihypertensive peptide and special device |
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| CN104195200A (en) * | 2014-09-01 | 2014-12-10 | 华东理工大学 | Method for preparing monosialotetra-hexosylganglioside (GM1) by applying recombinant sialidase converted gangliosides (GLS) |
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2016
- 2016-11-22 CN CN201611033617.9A patent/CN106591116A/en active Pending
Patent Citations (8)
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|---|---|---|---|---|
| US6107084A (en) * | 1995-10-19 | 2000-08-22 | Mitsuhiko Onda | Method for the preparation of an immobilized protein ultrathin film reactor and a method for a chemical reaction by using an immobilized protein ultrathin film reactor |
| US20030012798A1 (en) * | 2000-01-12 | 2003-01-16 | Tetsuro Ikekawa | Physiologically active substance eem-s originating in mushroms, process for producing the same and drugs |
| CN1266906A (en) * | 2000-03-01 | 2000-09-20 | 刘树军 | Process and equipment for preparing low-molecular peptide from sodium caseinate as raw material |
| CN1563405A (en) * | 2004-04-01 | 2005-01-12 | 广西大学 | Method for preparing peptide of soybean through continuous enzyme membrane reaction of soyabean protein |
| CN101724555A (en) * | 2009-12-18 | 2010-06-09 | 中国农业大学 | Whey protein antihypertensive peptide prepared by utilizing continuous enzyme membrane reactor and special device thereof |
| CN102453741A (en) * | 2010-10-18 | 2012-05-16 | 华中农业大学 | Method for preparing high-activity corn antihypertensive peptide and special device |
| CN103409315A (en) * | 2013-07-15 | 2013-11-27 | 重庆大学 | Reaction separating and coupling apparatus, and technology for preparation of gluconic acid from xylitol crystallization mother liquor |
| CN104195200A (en) * | 2014-09-01 | 2014-12-10 | 华东理工大学 | Method for preparing monosialotetra-hexosylganglioside (GM1) by applying recombinant sialidase converted gangliosides (GLS) |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116515628A (en) * | 2023-07-03 | 2023-08-01 | 鹤岗萃元科技产业发展有限公司 | Preparation facilities of full enzymatic synthesis diglyceride |
| CN116515628B (en) * | 2023-07-03 | 2023-09-19 | 鹤岗萃元科技产业发展有限公司 | Preparation facilities of full enzymatic synthesis diglyceride |
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Application publication date: 20170426 |
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