CN203598535U - Device convenient to switch flow path direction of Streamline column - Google Patents
Device convenient to switch flow path direction of Streamline column Download PDFInfo
- Publication number
- CN203598535U CN203598535U CN201320718736.3U CN201320718736U CN203598535U CN 203598535 U CN203598535 U CN 203598535U CN 201320718736 U CN201320718736 U CN 201320718736U CN 203598535 U CN203598535 U CN 203598535U
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- Prior art keywords
- tetra
- way valves
- interface
- post
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- Expired - Lifetime
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 6
- 239000002158 endotoxin Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 6
- 238000009826 distribution Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 231100000284 endotoxic Toxicity 0.000 description 2
- 230000002346 endotoxic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model provides a device convenient to switch a flow path direction of a Streamline column. By manufacturing combination of a group of valves, flow rate conversion of the Streamline column is facilitated and a flow path of a chromatographic column can be easily and rapidly converted. The device comprises a conversion flow path valve group which comprises a first four-path two-way valve, a second four-path two-way valve, a third four-path two-way valve, a plurality of gaskets, a plurality of connection pipes and a bracket; all pipelines need to be dismantled and connected again according to the liquid flow direction when the flow path is converted each time before the conversion flow path valve group is added to the Streamline column, this process is finished within a long period of time, the risk of bringing in microorganisms or endotoxin is generated, and therefore the safety of the product is affected. After the conversion flow path valve group is added, the flow path is converted just in a few seconds, the pipelines do not need to be connected again, and no exogenous microorganisms and endotoxin are brought in. Thus, the safety of the product is ensured.
Description
Technical field
The utility model relates to bio-pharmaceuticals apparatus field, and particularly the accessory technical field of separation chemistry product and natural products experiment, is specifically related to a kind of device of being convenient to switch Sreeamline post path direction.
Background technology
Chromatography is to utilize the difference of the distribution coefficient of each composition in some solvent in system and the capacity variance being adsorbed on adsorbent, thereby carries out the separating-purifying of chemical substance, and the method is widely used in biochemical industry field of medicaments.Column chromatography is process for separation and purification the most conventional in chromatographic technique, and the comprehensive function in system between character and the three of adsorbent, solvent, solute, the structure of chromatographic column itself is also one of influence factor.
Streamline post is the one of chromatographic column, and this chromatographic column adopts fluid bed mode purifying, and chromatographic column inner stuffing is in use dynamic.Other conventional chromatographic column inner stuffings are fixed.The albumen being applied in bio-pharmaceuticals is caught work, operating process relative complex.Be particle diameter and the Density Distribution of utilizing adsorbent, in the time that feed liquid pumps into from column bottom, adsorbent can expand in Streamline post, and mutual space increases, and has formed a gradient distribution, has limited the motion of particle.Liquid phase flows for laminar flow, thereby obtains stable expansion bed, and target product is attracted on the adsorbent in expansion bed, thereby has realized the initial gross separation of feed liquid.
Level pad: a kind of solution of preparing according to a certain percentage processing with sodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride, for expansion and the balance of Streamline post.
The use flow process of Streamline post as shown in Figure 1, generally can be divided into and start front sedimentation adsorbent, and adsorbent expands and balance, reinforced washing, four steps of wash-out packed bed.
Wherein, will be to the path direction change of chromatographic column 2 times in the later operation of second step (adsorbent expands and balance).When each conversion stream, all need all the removing again of pipeline to connect according to flow direction, this process need long time completes, and has the microorganism of introducing or endotoxic risk, and the security of product is impacted.
Utility model content
For addressing the above problem, the purpose of this utility model is to provide a kind of device of being convenient to switch Sreeamline post path direction.By manufacturing the combination of one group of valve, be convenient to the flow velocity conversion of Streamline post, can be than the stream that is easier to and changes quickly chromatographic column.
For achieving the above object, the structure that the utility model adopts is: comprising:
One conversion stream valve sets, comprises Yi No. tetra-two-way valves, Er No. tetra-two-way valves, San No. tetra-two-way valves, some pads, some tube connectors and a support;
The first interface that described Yi No. tetra-two-way valves have is communicated with by peristaltic pump A with level pad bucket; The second interface that San No. tetra-two-way valves have is communicated with by one No. 1 control valves can controlling switching with collecting vessel; The 3rd interface that Yi No. tetra-two-way valves have is communicated with the column cap of Streamline post; The 4th interface that San No. tetra-two-way valves have is communicated with the post tail of Streamline post; The 5th interface that Yi No. tetra-two-way valves have has the 6th interface with Er No. tetra-two-way valves and is communicated with; The 7th interface that Yi No. tetra-two-way valves have has the 8th interface with San No. tetra-two-way valves and is communicated with; The 9th interface of described Er No. tetra-two-way valves is communicated with the tenth interface of San No. tetra-two-way valves;
By fixing Yi No. tetra-two-way valves, Er No. tetra-two-way valves and San No. tetra-two-way valves supported of described support; Guarantee that by described pad conversion stream valve sets total interface is in sealing state.
Particularly, described Yi No. tetra-two-way valves, Er No. tetra-two-way valves and the selected specification of San No. tetra-two-way valves are internal diameter 6mm.
Further, the 11 interface that Er No. tetra-two-way valves have is communicated with blast pipe.
Further, separately there are a container for splendid attire 20% ethanolic solution and the column cap of Streamline post to be communicated with by one No. 2 control valves can controlling switching.
Further, between described container and the column cap of Streamline post, selectively access a peristaltic pump B.
The utlity model has following beneficial effect:
Before Streamline post adds and joins conversion stream valve sets, when each conversion stream, all need all the removing again of pipeline to connect according to flow direction, this process need long time completes, and has the microorganism of introducing or endotoxic risk, and the security of product is impacted.
After increasing conversion stream valve sets, conversion stream only needs the time in several seconds, and pipeline does not need to reconnect and do not have ectogenic microorganism and endotoxin is introduced yet.The safety of the product of guaranteeing.
Accompanying drawing explanation
Fig. 1 is that general Streamline post uses schematic flow sheet.
Fig. 2 is connection and the running schematic diagram of the whole stream of Streamline post in " adsorbent expands and balance " step in the utility model embodiment.
Fig. 3 be in the utility model embodiment in " wash-out packed bed " step the connection of the whole stream of Streamline post and running schematic diagram.
In figure: 1-loading pin; 2-level pad bucket; 3-peristaltic pump A; 4-conversion stream valve sets; 41-Yi No. tetra-two-way valves; 411-first interface; 412-the 3rd interface; 413-the 5th interface; 414-the 7th interface; 42-Er No. tetra-two-way valves; 421-the 6th interface; 422-the 11 interface; 423-the 9th interface; 43-San No. tetra-two-way valves; 431-the tenth interface; 432-the 8th interface; 433-the 4th interface; 434-the second interface; 5-streamline post; 51-column cap; 52-post tail; 6-peristaltic pump B; 7-2 control valve; 8-20% ethanolic solution; 9-1 control valve; 10-drain pipe; 11-collecting vessel.
The specific embodiment
For above-mentioned feature and advantage of the present utility model can be become apparent, special embodiment below, and coordinate appended graphic being described in detail below.
Embodiment 1:
One group of Streamline column device, packs a conversion stream valve sets 4 into, comprises Yi No. tetra-two-way valves 41, Er No. tetra-two-way valves 42, San No. tetra-two-way valves 43, some pads, some tube connectors and a support;
The first interface 411 that described Yi No. tetra-two-way valves 41 have is communicated with by peristaltic pump A3 with level pad bucket 2 or liquid storing barrel; The second interface 434 that San No. tetra-two-way valves 43 have is communicated with by one No. 1 control valves 9 can controlling switching with collecting vessel 11; The 3rd interface 412 that Yi No. tetra-two-way valves 41 have is communicated with the column cap 51 of Streamline post 5; The 4th interface 433 that San No. tetra-two-way valves 43 have is communicated with the post tail 52 of Streamline post 5; The 5th interface 413 that Yi No. tetra-two-way valves 41 have has the 6th interface 421 with Er No. tetra-two-way valves 42 and is communicated with; The 7th interface 414 that Yi No. tetra-two-way valves 41 have has the 8th interface 432 with San No. tetra-two-way valves 43 and is communicated with; The 9th interface 423 of described Er No. tetra-two-way valves 42 is communicated with the tenth interface 431 of San No. tetra-two-way valves 43;
By fixing Yi No. tetra-two-way valves 41, Er No. tetra-two-way valves 42 and San No. tetra-two-way valves 43 supported of described support; Guarantee that by described pad conversion stream valve sets 4 total interfaces are in sealing state.
Described Yi No. tetra-two-way valves 41, Er No. tetra-two-way valves 41 and the selected specification of San No. tetra-two-way valves 43 are internal diameter 6mm.
Afterwards, the washing of feeding in raw material, loading pin 1 inserts in the supernatant in liquid storing barrel, and drain pipe inserts in collecting vessel 11, opens peristaltic pump A3, starts loading.End of the sample is closed peristaltic pump A3.
Afterwards, carry out wash-out packed bed, conversion stream valve sets 4 is switched to position as shown in Figure 3, level pad is flowed into from column cap 51, post tail 52 flows out.Open control valve 7 No. 2, and silicone tube installs to peristaltic pump B6 above, opens switch herein, adjusting flow velocity is 20ml/min, makes 20% ethanol in storage bottle, squeeze into piston top hydraulic part, until under piston along to 21.5cm place, stop peristaltic pump B6, unload silicone tube, close control valve 7 No. 2.
Afterwards, re-start expansion and the equilibrium step of adsorbent after finishing using.
As in use occurred bubble, Er No. tetra-two-way valves 42 are switched to blast pipe and carry out exhaust.
Whole operating process, conversion path direction only needs time in several seconds, and pipeline do not need to reconnect, and has so just stopped the possibility of ectogenic microorganism and endotoxin introducing.The safety of the product of guaranteeing.
Claims (6)
1. a device of being convenient to switch Sreeamline post path direction, is characterized in that, comprising:
One conversion stream valve sets, comprises Yi No. tetra-two-way valves, Er No. tetra-two-way valves, San No. tetra-two-way valves, some tube connectors and a support;
Described Yi No. tetra-two-way valves, Er No. tetra-two-way valves and San No. tetra-two-way valves are fixed on support successively;
The first interface that described Yi No. tetra-two-way valves have is communicated with by peristaltic pump A with level pad bucket or liquid storing barrel; The second interface that San No. tetra-two-way valves have is communicated with by one No. 1 control valves can controlling switching with collecting vessel; The 3rd interface that Yi No. tetra-two-way valves have is communicated with the column cap of Streamline post; The 4th interface that San No. tetra-two-way valves have is communicated with the post tail of Streamline post; The 5th interface that Yi No. tetra-two-way valves have has the 6th interface with Er No. tetra-two-way valves and is communicated with; The 7th interface that Yi No. tetra-two-way valves have has the 8th interface with San No. tetra-two-way valves and is communicated with; The 9th interface of described Er No. tetra-two-way valves is communicated with the tenth interface of San No. tetra-two-way valves.
2. the device of being convenient to switch Sreeamline post path direction according to claim 1, is characterized in that, described Yi No. tetra-two-way valves, Er No. tetra-two-way valves and the selected specification of San No. tetra-two-way valves are internal diameter 6mm.
3. the device of being convenient to switch Sreeamline post path direction according to claim 1, is characterized in that, the 11 interface that Er No. tetra-two-way valves have is communicated with a blast pipe.
4. the device of being convenient to switch Sreeamline post path direction according to claim 1, is characterized in that, separately has a container for splendid attire 20% ethanolic solution and the column cap of Streamline post to be communicated with by one No. 2 control valves can controlling switching.
5. the device of being convenient to switch Sreeamline post path direction according to claim 4, is characterized in that, between described container and the column cap of Streamline post, selectively accesses a peristaltic pump B.
6. the device of being convenient to switch Sreeamline post path direction according to claim 1, is characterized in that, also comprises some pads, guarantees that the total interface of conversion stream valve sets is in sealing state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320718736.3U CN203598535U (en) | 2013-11-14 | 2013-11-14 | Device convenient to switch flow path direction of Streamline column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320718736.3U CN203598535U (en) | 2013-11-14 | 2013-11-14 | Device convenient to switch flow path direction of Streamline column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203598535U true CN203598535U (en) | 2014-05-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320718736.3U Expired - Lifetime CN203598535U (en) | 2013-11-14 | 2013-11-14 | Device convenient to switch flow path direction of Streamline column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203598535U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105664527A (en) * | 2016-01-27 | 2016-06-15 | 李广良 | Continuous chromatographic separation device and method using device |
| CN113915177A (en) * | 2021-09-16 | 2022-01-11 | 利穗科技(苏州)有限公司 | Electro-hydraulic servo driving device and chromatography equipment |
-
2013
- 2013-11-14 CN CN201320718736.3U patent/CN203598535U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105664527A (en) * | 2016-01-27 | 2016-06-15 | 李广良 | Continuous chromatographic separation device and method using device |
| CN113915177A (en) * | 2021-09-16 | 2022-01-11 | 利穗科技(苏州)有限公司 | Electro-hydraulic servo driving device and chromatography equipment |
| CN113915177B (en) * | 2021-09-16 | 2024-05-14 | 利穗科技(苏州)有限公司 | Electrohydraulic servo driving device and chromatographic equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No.1 and No.2 Juli Road, No.280, Free Trade Pilot Area, Pudong New Area, Shanghai, 2003 Patentee after: TASLY PHARMACEUTICAL GROUP Co.,Ltd. Address before: 201203 No. 280 Curie Road, Zhangjiang High-tech Park, Pudong New Area, Shanghai Patentee before: SHANGHAI TASLY PHARMACEUTICAL Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140521 |