CN106577657A - Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid - Google Patents
Method for relieving panax pseudoginseng autotoxin damage by exogenously adding ascorbic acid Download PDFInfo
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- CN106577657A CN106577657A CN201611114645.3A CN201611114645A CN106577657A CN 106577657 A CN106577657 A CN 106577657A CN 201611114645 A CN201611114645 A CN 201611114645A CN 106577657 A CN106577657 A CN 106577657A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for relieving panax pseudoginseng autotoxin damage by exogenously adding an ascorbic acid and belongs to the technical field of plant protection. The concrete technical scheme of the method is as follows: carrying out seed soaking treatment on a panax pseudoginseng seed by using an L-ascorbic acid solution with the concentration of 0.1-50.0mg/L; or dissolving the L-ascorbic acid solution into a seed coating celest to prepare an L-ascorbic acid seed coating with the concentration of 50.0-2500.0mg/L, and then, carrying out coating treatment on the panax pseudoginseng seed; or carrying out root soaking treatment on a seedling by using the L-ascorbic acid solution with the concentration of 0.1-50.0mg/L. The beneficial effects of the method lie in that due to the adoption of the method for exogenously adding the ascorbic acid, the resistance of panax pseudoginseng is improved, the probability of root rot hazards caused by cell injury generated by autotoxins in a panax pseudoginseng growth process is reduced, the use amount of a pesticide is reduced, and the method is a measure for ecologically, safely and effectively relieving continuous cropping obstacles.
Description
Technical field
The present invention relates to a kind of utilization external source addition ascorbic acid alleviates the method that Radix Notoginseng suppression harmful bacteria is damaged, platymiscium is protected
Shield technical field.
Background technology
Radix Notoginseng is one of large rare Chinese medicine of China, but continuous cropping obstacle seriously restricts the production of Radix Notoginseng.Existing research table
It is bright, planted the soil of Radix Notoginseng after 10 to 20 years can not continuous cropping, continuous cropping obstacle has become restriction Radix Notoginseng industry and develops in a healthy way
Principal element.For many years, numerous scholars surround the reason for causing notoginseng obstacle and expand in-depth study, it is believed that continuous cropping
Obstacle mainly with the infecting of pine root fungus, the accumulation of suppression harmful bacteria, the deterioration of soil physical and chemical property and soil microflora
The factors such as change are relevant.The research of this seminar shows that the saponinss suppression harmful bacteria secreted during Panax notoginseng Growth into soil is
The major incentive for causing notoginseng obstacle seriously to occur.Especially emerging the stage in seed and seedling, Radix Notoginseng root system opposing energy
Power is weak, and the suppression harmful bacteria remained in soil has a strong impact on emerging and growing for seed seedling.These saponinss suppression harmful bacterias are to three
Seven seeds are sprouted and root growth has obvious Auto toxicity, cause root cell dead.It is more favourable after root cell death
Infecting in pathogen, increases the generation of root rot.Seminar carries out Difference of Metabolism analysis and finds using GC-TOF-MS technologies,
Processing from malicious saponin after root system polyphenoils (L-AA) content in cell body can be caused to decline rapidly.From at malicious saponin
Root cell active oxygen (ROS) can be caused after reason to burst out, make cell damage.In order to maintain the ROS in cell body to balance, cell meeting
Produce serial antioxidase and polyphenoils to eliminate unnecessary ROS, maintain cell Redox balance.Ascorbic acid is cell
The important antioxidant for producing in vivo, plays an important role in cell ROS equilibrium process is maintained.Suppression harmful bacteria processes Radix Notoginseng
Reduce intracellular Antioxidants after root system, disturb the redox equilibrium of root system, cause living in root cell
Property oxygen a large amount of accumulation cause oxidative damage, ultimately result in root cell dead.Therefore, a kind of method is found thin to improve root system
The oxygen radical removing ability of born of the same parents, the oxygen-derived free radicals for recovering to be caused by suppression harmful bacteria accumulate imbalance state be overcome from poison damage,
Mitigate the important channel of notoginseng obstacle.
Result of the present invention according to Auto toxicity Mechanism Study, there is provided one kind alleviates Radix Notoginseng using external source addition ascorbic acid
The method that suppression harmful bacteria is damaged.The method can safely and effectively eliminate Radix Notoginseng from toxicity damage, alleviate notoginseng obstacle.Scripture is offered
Retrieval, has no and identical open source literature of the present invention report.
The content of the invention
The cellular oxidation injury that the present invention causes for suppression harmful bacteria during Panax notoginseng Growth, there is provided one kind is added using external source
Plus antioxidants ascorbic acid come adjust cell Redox balance, recover root system redox ability, mitigate suppression harmful bacteria wound
Harmful method.
The present invention concrete technical scheme be：Using the L-AA solution that concentration is 0.1～50.0mg/L to Radix Notoginseng
Seed carries out seed soaking；Or be configured to during L-AA is dissolved in when seed coat agent fits happy containing concentration be 50.0～
Then Panax notoginseng seeds are coated process by the seed coat agent of the L-AA of 2500.0mg/L；Or using concentration be 0.1～
The L-AA solution of 50.0mg/L carries out soaking root process to seedling.These processing modes can effectively alleviate the poison certainly of Radix Notoginseng
Injury, promotes emerging and growing for Radix Notoginseng.
Described L-AA seed-soaking is processed as：Using the deionized water that percent by volume is 0.01% Tween 80
L-AA is configured to into the solution of 0.1～50.0mg/L of concentration, then Panax notoginseng seeds is put in solution and is soaked 10min,
Taking-up is dried and sowed after the surface of the seed moisture.
Described L-AA seed pelleting is processed as：It is 0.01% Tween 80 by ascorbic acid percent by volume
In making an addition to after deionized water dissolving when Radix Notoginseng seed coat agent fits happy, be configured to containing L-AA concentration be 50.0～
The seed coat agent of 2500.0mg/L, then according to pharmacopoeia mass ratio 1:50 ratio is coated process, and the surface of the seed seed coat agent dries in the air
Sow after dry.
Described L-AA seedling leaching root is processed as：Using the deionized water that percent by volume is 0.01% Tween 80
Dissolving L-AA, is configured to the solution of 0.1～50.0mg/L of concentration, then Radix Notoginseng seedling root system is put in solution and is soaked
10min, taking-up is dried and transplanted after seedling root system surface moisture.
The beneficial effects of the present invention is：
1st, the external source addition manner for soaking root using seed-soaking, seed coat agent coating and seedling supplements Radix Notoginseng rhizosphere L- Vitamin Cs
The content of acid, overcomes the oxygen radical removing brought due to antioxidant reduction caused by suppression harmful bacteria in Radix Notoginseng root cell
The problem that ability declines；Strengthen the oxidation resistance of Panax notoginseng seeds and seedling root system simultaneously, mitigate the injury of suppression harmful bacteria.
2nd, by the addition of Exogenous Antioxidants L-AA, the resistance of Radix Notoginseng is enhanced, reduces Panax notoginseng Growth mistake
Because suppression harmful bacteria causes cell damage so as to increase the probability of pine root fungus harm in journey, the usage amount of pesticide is reduced, be
A kind of measure that is ecological, safely, effectively alleviating notoginseng obstacle.
Specific embodiment：
Embodiment 1:Strengthen root cell antioxidation detoxification ability using external source addition ascorbic acid
After annual Radix Notoginseng seedling grows 7 months in test cool canopy, the Radix Notoginseng plant for choosing health takes back laboratory, uses
Soil on root is rinsed well and carries out liquid culture with being put in 200mL tissue culture bottles after aseptic water washing 3 times again by tap water.In order to
Excluded volume percentage ratio is impact of 1 ‰ methanol to Radix Notoginseng root system, and the first that percent by volume is 1 ‰ is added in all process
Alcohol.Test arranges altogether 5 process, i.e., with concentration 1.0mg/L Rg10h, 3h, 12h, 24h and 48h are processed respectively.Each process sets
4 repetitions are put, each repetition is put into 8 Radix Notoginseng Seedlings.Test is processed using inverted order method, all process water in sterilized water
Training 48h after sample, first by it is all process be incubated in sterilized water (100mL/ bottles), then the distance sampling time 3,12,24,
The certainly malicious saponin Rg that 0.1mL concentration is 1.0mg/L is separately added into during 48h1, control is the deionized water containing 1 ‰ methanol.Process
Radix Notoginseng Seedling afterwards is placed in 12h alternation of light and darkness culture in growth cabinet, and incubator day temperature is 25 DEG C, and nocturnal temperature is 20
℃.Respectively 4 plants of Radix Notoginseng Seedlings of random choose take its fibrous root for each repetition during sampling, at once with liquid nitrogen freezing, are placed in -80 DEG C of refrigerators
Preserve.Frozen sample extraction simultaneously after derivatization, is measured using the relative amount change of GC-TOF-MS Ascorbic Acids,
Carry out the absolute quantification analysis of ascorbic acid in different disposal time root system using the method for HPLC combined standard product simultaneously.Separately
Outward, Radix Notoginseng is tied up to from malicious saponin Rg10.1,1.0,10.0,50.0 and 100.0mg/L Vitamin C is added while process
Acid solution, sampled after 12 hours, with Scavenger of ROS related enzyme systems APX, SOD, the content of POD, GST in measure root system tissue
Change.
As a result show：From malicious saponin Rg1After processing Radix Notoginseng root system, the relative amount for either being determined with GC-TOF-MS becomes
Change, or under showing that ascorbic acid concentrations are after 3h is processed drastically in root system tissue with the result of HPLC absolute quantification analysis
Adjust, then begin to slow rising, but be substantially less than the process (table 1) of 0h.From malicious saponin Rg1Process 12 hours after Radix Notoginseng root system
Active o content in root system tissue significantly increases, and shows that root cell is subject to oxidative damage (table 2).From malicious saponin Rg1Process
While external source addition 0.1～50.0mg/L of concentration ascorbic acid process can significantly improve it is related to oxygen scavenging activity in root tissue
Enzyme system APX, SOD, the activity of POD, GST, so as to reduce the active o content in tissue, the oxidation for mitigating suppression harmful bacteria is damaged
Wound.But can not substantially reduce when ascorbic acid concentrations are increased to 100.0mg/L from toxicity damage (table 2).
The embodiment result shows, comprehending from malicious saponin causes under polyphenoils ascorbic acid content is notable in root system
Drop, root system is reduced to the resistivity of suppression harmful bacteria.But external source addition ascorbic acid can significantly increase the antioxygen of root system after processing
Change ability, mitigates harm of the suppression harmful bacteria to root system.
The certainly malicious saponin Rg of table 11Process ascorbic acid content change in Radix Notoginseng root system after different time
The external source of table 2 addition ascorbic acid processes the impact to Radix Notoginseng cell accumulated active oxygen and antioxidation relevant enzyme
Embodiment 2:The emergence rate of Radix Notoginseng is improved using the process of ascorbic acid seed-soaking
The moisture that sarcocarp dries the surface of the seed is removed after Panax notoginseng seeds collection, is 0.01% Tween 80 with percent by volume
Deionized water compound concentration is 0,0.1,1.0,10.0,50.0, the ascorbic acid solution of 100.0mg/L immersion seed 10min, take
The surface of the seed moisture is dried after going out.Then it is the certainly malicious saponin Rg of 1.0mg/L the Panax notoginseng seeds after process to be seeded in into addition concentration1
Soil in, planted in being not added with from the new soil of malicious saponin as control treatment with the Panax notoginseng seeds of unused ascorbic acid immersion.Often
Individual process arranges 3 repetitions, 1000 seeds of each repeat sowing.After planting conventionally it is managed, treats at control
Reason emergence rate reaches more than 60% and investigates emergence rate, compares antioxidant and processes the impact emerged to Panax notoginseng seeds and grow.
As a result show (table 3), Panax notoginseng seeds are seeded in and are not added with from malicious saponin Rg1Soil in growth it is normal, but soil
Suppression harmful bacteria Rg of middle addition 1.0mg/L1The emerging of Panax notoginseng seeds, the growth of plant height, root length and root weight can be significantly inhibited afterwards to refer to
Mark.When can significantly alleviate after the ascorbic acid seed soaking of Panax notoginseng seeds 0.1～50.0mg/L of concentration from malicious saponin to emerging
With the depression effect of growth.After especially being processed with the ascorbic acid of 1.0～50.0mg/L of concentration, the root length and root weight of Panax notoginseng seeds
It is also significantly higher than the control treatment for being not added with suppression harmful bacteria.But after Panax notoginseng seeds are processed with the ascorbic acid of 100.0mg/L not
Auto toxicity can be relieved, this shows that mitigation of the ascorbic acid to Auto toxicity has obvious dosage effect.It is above-mentioned
Result of the test shows that Panax notoginseng seeds are carried out using the ascorbic acid of 0.1～50.0mg/L of concentration can be effective after seed soaking
Alleviate the harm of suppression harmful bacteria.
The impact that the ascorbic acid solution seed soaking of table 3 is sprouted to Panax notoginseng seeds and grown
Embodiment 3:The process of L-AA seed pelleting promotes emerging for Panax notoginseng seeds
Remove sarcocarp after Panax notoginseng seeds collection and dry the moisture of the surface of the seed, be utilized respectively containing ascorbic acid concentrations be 0,
5.0th, 50.0,500.0,2500.0,5000.0mg/L it is suitable happy when seed coat agent according to pharmacopoeia mass ratio 1:50 ratio is mixed into
Row Cotton seeds, seed coat agent is sowed after drying.It is the certainly malicious soaps of 1.0mg/L that the Panax notoginseng seeds of different disposal are seeded in into addition concentration
Glycosides Rg1Soil in, it is right that the Panax notoginseng seeds processed during only fitting happy with seed coat agent are planted in being not added with from the new soil of malicious saponin
According to process.Each processes and arranges 3 repetitions.1000 seeds of each repeat sowing.After planting conventionally it is managed,
Treat that control treatment emergence rate reaches more than 60% and investigates emergence rate, compare ascorbic acid Cotton seeds and Panax notoginseng seeds are emerged and is given birth to
Long impact.
As a result show (table 4), suppression harmful bacteria Rg is added in soil1Afterwards 34% is reached to the suppression ratio that Radix Notoginseng emerges.But three
Seven seeds after the seed coat agent coating containing concentration 5.0～2500.0mg/L ascorbic acid with can significantly alleviate from malicious saponin to emerging
Inhibitory action.Can be relieved Auto toxicity as 5.0mg/L containing ascorbic acid concentrations in seed coat agent, but plant height, root length and
Root escheat is substantially less than control treatment.After seed is with containing concentration 50.0～2500.0mg/L ascorbic acid Cotton seeds, Radix Notoginseng
The emergence rate of seed is completely recovered to be not added with the level of suppression harmful bacteria soil.But when concentration increases to 5000.0mg/L, three
The root of seven plant is heavy, root length and root are significantly inhibited again.This shows that ascorbic acid Cotton seeds concentration needs control 50.0
In the range of～2500.0mg/L.
The external source of table 4 adds impact of the ascorbic acid solution Cotton seeds to Seed Germination and growth
Embodiment 4:The emergence rate of Radix Notoginseng seedling is improved using the process of Vitamin C acidleach root
The soil that root system sticks is removed after the excavation of Radix Notoginseng seedling, with the deionized water that percent by volume is 0.01% Tween 80
Compound concentration is 0,0.1,1.0,10.0,50.0, the ascorbic acid solution immersion seedling 10min of 100.0mg/L, taking-up dries root
It is to transplant after surface moisture.It is the certainly malicious saponin Rg of 1.0mg/L that transplantation of seedlings is planted in concentration is added1Soil in, with unused Vitamin C
It is control treatment that the Radix Notoginseng seedling of acid soak is planted in being not added with from the new soil of malicious saponin.Each processes and arranges 3 repetitions.Each
Repeat to transplant 100 plants of seedlings.After planting conventionally it is managed, treats that control treatment emergence rate reaches more than 60% investigation
Emergence rate, compares antioxidant leaching root and processes the impact emerged to Radix Notoginseng and grow.
As a result show (table 5), suppression harmful bacteria Rg is added in soil1After significantly suppress Radix Notoginseng seedling emergence rate, plant height, strain
The growth indexes of weight, root weight and root length.The ascorbic acid of seedling 0.1～50.0mg/L of concentration can delay to some extent after processing
Solution is from malicious saponin to the inhibitory action emerged.But concentration can suppress the growth of Radix Notoginseng when reaching 100.0mg/L.This shows Vitamin C
Acidleach root concentration for the treatment of needs control in the range of 0.1～50.0mg/L.
The ascorbic acid solution of table 5 leaching root processes the impact to Radix Notoginseng seedling early growth
Embodiment 5:The emergence rate of Radix Notoginseng seedling is improved using ascorbic acid root irrigation
Using the EXPERIMENTAL DESIGN of random district's groups.Radix Notoginseng is transplanted to the new soil and addition concentration for not planting Radix Notoginseng respectively
For the certainly malicious saponin Rg of 1.0mg/L1It is new soil in.Be utilized respectively in 7 days after Radix Notoginseng kind transplantation of seedlings deionized water preparation concentration be
0th, 0.1,1.0,10.0,50.0 and 100.0mg/L ascorbic acid solution root irrigation is once.Again with identical after Radix Notoginseng emerges
The ascorbic acid solution continuous pouring of concentration 3 times, every minor tick 7 days.Every Radix Notoginseng pouring root amount is 5mL.Each processes and arranges 3 times
Repeat, the cell that each repeats plants 200 plants of Radix Notoginseng.Emergence rate and Radix Notoginseng life are investigated after emerging according to five point sampling after 60 days
Long index of correlation, compares impact of the ascorbic acid process to Panax notoginseng Growth.
As a result (table 6) is shown, Radix Notoginseng emerges and grows normal in the case where soil is not added with suppression harmful bacteria.Add in soil
Enter suppression harmful bacteria Rg1Radix Notoginseng is emerged afterwards and is grown with significant suppression.Using the ascorbic acid of 0.1～10.0mg/L of concentration
Can significantly alleviate after process from malicious saponin to the inhibitory action emerging and grow, its emergence rate and growth indexes are suitable with control very
To better than control.But to poison certainly without significant remission effect after concentration increases to 50.0 and 100.0mg/L.This shows to utilize
The ascorbic acid root irrigation of 0.1～10.0mg/L can effectively alleviate harm of the suppression harmful bacteria to Radix Notoginseng.
Impact of the ascorbic acid solution root irrigation of table 6 to Radix Notoginseng seedling early growth
Claims (1)
1. a kind of utilization external source addition ascorbic acid alleviates the method that Radix Notoginseng suppression harmful bacteria is damaged, it is characterised in that the tool of the method
Body technique scheme is：Seed soaking is carried out to Panax notoginseng seeds using the L-AA solution that concentration is 0.1～50.0mg/L；Or
It is configured to during L-AA is dissolved in when seed coat agent fits happy containing the L-AA that concentration is 50.0～2500.0mg/L
Seed coat agent, then Panax notoginseng seeds are coated with process；Or using the L-AA solution that concentration is 0.1～50.0mg/L
Seedling is carried out to soak root process；Wherein：
Described L-AA seed-soaking is processed as：Using the deionized water that percent by volume is 0.01% Tween 80 by L-
Ascorbic acid is configured to the solution of 0.1～50.0mg/L of concentration, then Panax notoginseng seeds is put in solution and soaks 10min, takes out
Dry and sowed after the surface of the seed moisture；
Described L-AA seed pelleting is processed as：By ascorbic acid percent by volume be 0.01% Tween 80 go from
In making an addition to after sub- water dissolution when Radix Notoginseng seed coat agent fits happy, it is 50.0～2500.0mg/L to be configured to containing L-AA concentration
Seed coat agent, then according to pharmacopoeia mass ratio 1:50 ratio is coated process, and the surface of the seed seed coat agent is sowed after drying；
Described L-AA seedling leaching root is processed as：Using the deionized water dissolving that percent by volume is 0.01% Tween 80
L-AA, is configured to the solution of 0.1～50.0mg/L of concentration, then Radix Notoginseng seedling root system is put in solution and is soaked
10min, taking-up is dried and transplanted after seedling root system surface moisture.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646754A (en) * | 2021-01-22 | 2021-04-13 | 云南农业大学 | Burkholderia and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646754A (en) * | 2021-01-22 | 2021-04-13 | 云南农业大学 | Burkholderia and application thereof in preventing and treating main pathogenic bacteria of panax notoginseng root rot |
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