CN106577263B - A kind of rape chemical hybridizing agent accuracy pesticide applying method - Google Patents
A kind of rape chemical hybridizing agent accuracy pesticide applying method Download PDFInfo
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- CN106577263B CN106577263B CN201611176757.1A CN201611176757A CN106577263B CN 106577263 B CN106577263 B CN 106577263B CN 201611176757 A CN201611176757 A CN 201611176757A CN 106577263 B CN106577263 B CN 106577263B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of rape chemical hybridizing agent accuracy pesticide applying methods, are pierced into caulome at rape stem using small wood cone point, pipettor is recycled SX-1 aqueous solution to be injected into the small holes that rape stem is pierced；It can induce rape after 8~9d and male sterility occur, its sterile plant rate is up to 100%, semisterility strain rate and phytotoxicity strain rate are 0, sterile 21~24d of lasting period, and the influence to rape is smaller, plant height, main inflorescence length, main inflorescence silique number, seed number per pod and single plant yield and control no significant difference in spontaneous pollination.Present invention application dosage is accurate, the sterile effect of induced male is good, and the lasting period is long, absolutely not phytotoxicity, and the factors such as operation and implementation result climate environment influence very little, and easy to operate, special training is not necessarily to, without special protection equipment；The present invention is suitable for killing agent progress indirect labor's emasculation crossbreeding using rape and small area kills cenospecies test production of hybrid seeds work etc..
Description
Technical field
The invention belongs to technical field of crop propagation, are related to a kind of rape chemical hybridizing agent accuracy pesticide applying method.
Background technique
Crop male sterility is the basis for producing hybrid seed, from the point of view of domestic and international progress at present, although crop is miscellaneous
Kind use of advantage approach has cytoplasmic male sterility (CMS), nuclear male sterility (GMS), is selfed not affine (SI) and chemistry
Gametocide (CH) etc., but be method the most cost-effective using chemical emasculation male sterility；It is induced with chemical hybridizing agent (CHA) male
Property infertility is also one of the important channel that rape heterosis utilizes, and the method eliminates the breeding of sterile line, greatly simplified
The procedure of breeding, breeding cycle is short, while comparatively small to the dependence of genotype, can more freely select kind to prepare miscellaneous
Kind.Therefore, theoretically we can according to need and choose any kind as sterile line and restorer, and being quickly obtained has height
The cenospecies of the merits such as production, high-quality, degeneration-resistant, disease-resistant.By nearly research in 40 years, China utilizes in rape chemical emasculation and grinds
Study carefully and occupy first place in the world, the novel rape of open report kill agent have KMS-1, EN, SX-1, EXP, determine army 1, ESP, change are killed
Spirit, BHL, GS-1 and tribenuron-methyl etc..SX-1 be studied, screened by Shaanxi cross-bred rape research center, made one kind it is novel
Chemical hybridizing agent, using technology in acquisition Chinese invention patent (patent No.: ZL03105389.0) in 2006, hybridization agent tool
There is the features such as efficient, micro- poison, noresidue, low cost, gametocide thorough, using simplicity, the change is killed agent and is mainly widely used at present
Preparation kills hybrid；Solve the problems, such as the micro mist that three systems and crossbreeding of two-series occur；Two be that Genetic Sterility solves to transfer in female parent
Fertile plant；Artificial emasculation is replaced in terms of conventional cross-breeding, improves seed selection efficiency.Since two thousand in Qin-you No.7, Qin You
No. 10, using SX-1 control more than 80,000 mu of micro mist in the Rapeseed CMSs production of hybrid seeds such as Qinzayou No. 1, make hybrid seed recovery rate improve 5~
10 percentage points, seed purity is stablized 95% or more；And using the change kill technology cultivated Qin You 33, Qin You 19,
11 chemical hybridization kinds such as Qin Rong 2, miscellaneous oily No. 4 of the Qin, being used successfully to kill 7500 mu of the production of hybrid seeds or so, and change kills cenospecies and adds up to push away
Wide more than 400 ten thousand mu of area.But the rape male sterilant of open report is higher using technical requirements at present, especially to making
Dosage and environmental condition are more demanding, are mainly constrained to pesticide application technology bottleneck；Currentization kills used application method
Usually spray-on process and stem brush method, the usually used sprayer of spray-on process is of low quality, and application dosage inaccuracy error is larger, applies
Medicine is uneven, resprays and will lead to phytotoxicity, and drain spray, which will lead to, is not achieved gametocide purpose, and vulnerable to such environmental effects, especially
It is that wind and rainfall can cause damages to neighbouring sensitive crop if wind-force is larger when application will lead to medical fluid drift；After application
4~6h rainfall can have an impact gametocidal effect；In addition rape variety leaf area, wax powder layer, stomatal frequency and haze weather etc.
The attachment and absorption that factor all can kill agent to change have a certain impact, and it is undesirable to kill effect so as to cause change；Although stem brush method by
Environment influence it is smaller, but there is also application dosage inaccuracy error it is larger, cause generate phytotoxicity or gametocidal effect it is bad, these because
The limitation of element kills agent to rape and the popularization and application of cenospecies cause greatly to negatively affect.
And current research mostly concentrates on dosage that newcooperative medical system kills the exploitation of agent and use, on period, and killed for changing
Seldom, therefore, the pesticide application technology method that rape kills agent is current one urgently to be solved and important asks for the method for administration research of agent
Topic, researching and developing novel pesticide application technology is one of rape Chemical Castration Technology main research.
Summary of the invention
The purpose of the present invention is to provide a kind of rape chemical hybridizing agent accuracy pesticide applying methods, it is intended to solve in current rape
Change kills used by agent is administered under application method that application dosage inaccuracy error is larger, application is uneven, cause phytotoxicity occur or
The problems such as gametocidal effect is undesirable, the application method that agent is killed in change limit rape and kill agent and cenospecies further genralrlization and answer
With.
The invention is realized in this way a kind of rape chemical hybridizing agent accuracy pesticide applying method, the rape chemical hybridizing agent
Accuracy pesticide applying method utilizes a root long 10cm, the small wood that the front end diameter 0.55cm is cone；The high 1.5cm of cone, in oil
The high 25cm of tender flower stalk, bud 2mm period downward 45 degree of piercings caulome at rape stem 10cm~15cm with small wood cone point；Thorn
The depth entered, which is subject to, does not pierce through rape stem；Recycling pipettor 25ul, change that concentration is 100mg/L, to kill agent SX-1 water-soluble
Liquid is injected into the small holes that rape stem is pierced.
Further, the front end is processed into the circle of high 1.5cm for the cone in the small wood of cone using turning pencil knife
Cone.
Further, describedization kills agent SX-1 and is configured to concentration 100mg/L with sterile water.
Further, the pipettor uses 20ul~100ul single channel manual controlled variable pipettor.
Another object of the present invention is to provide a kind of to be assisted using above-mentioned rape chemical hybridizing agent accuracy pesticide applying method
The rape of artificial emasculation crossbreeding.
Another object of the present invention, which is to provide, a kind of utilizes above-mentioned rape chemical hybridizing agent accuracy pesticide applying method to carry out facet
Productization kills the rape of the cross experiment production of hybrid seeds.
Compared with prior art, outstanding advantages of the invention are: application dosage is accurate, the sterile effect of induced male is good, holds
The effect phase is long, absolutely not phytotoxicity, and the factors such as operation and implementation result climate environment influence very little, and easy to operate, without special
Door training, is not necessarily to special protection equipment.
Can induce rape after 8~9d of the present invention and male sterility occur, sterile plant rate up to 100%, semisterility strain rate and
Phytotoxicity strain rate is 0, sterile 21~24d of lasting period, and the influence to rape is smaller, plant height, main flower in spontaneous pollination
Sequence length, main inflorescence silique number, seed number per pod and single plant yield and control no significant difference.The present invention is suitable for killing agent using rape
It carries out indirect labor's emasculation crossbreeding and small area kills cenospecies test production of hybrid seeds work etc..
Detailed description of the invention
Fig. 1 is rape chemical hybridizing agent accuracy pesticide applying method flow diagram provided in an embodiment of the present invention.
Fig. 2 is the structural schematic diagram that one end provided in an embodiment of the present invention is cone small wood.
Fig. 3 is rape note stem method male sterility inducing effect figure provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Application principle of the invention is described in detail with reference to the accompanying drawing.
As shown in Figure 1, rape chemical hybridizing agent accuracy pesticide applying method provided in an embodiment of the present invention, comprising:
S101: using root long 10cm or so, the front end diameter 0.55cm be cone small wood, the high 1.5cm of cone,
In high 25cm of rape a kind of sedge or so, bud 2mm or so period is with small wood cone point downward 45 degree at 10~15cm of rape stem
It is pierced into caulome, the depth of piercing, which is subject to, does not pierce through rape stem.
S102: recycle pipettor that 25ul, the change that concentration is 100mg/L are killed agent SX-1 aqueous solution and be injected into rape stem
In the small holes pierced.
High 1.5cm is processed into turning pencil knife using the wooden stick of root long 10cm or so, diameter 0.55cm in one end by the present invention
Cone.
Present invention useization kills agent SX-1 and is configured to concentration 100mg/L with sterile water, and 1mL is taken to be divided in the centrifugation of 1.5ml
Guan Zhong.Every pipe can infuse 40 plants of stem rape.
The present invention is also known as liquid-transfering gun using pipettor, is a kind of utensil for quantitative transfer liquid, 20~100ul single-pass
The manual controlled variable pipettor in road.
Application Example one: change the preparation for killing tri- kinds of agent SX-1 different application method medical fluids
(1) change the preparation for killing agent SX-1 foliar spray medicament: weighing kills agent (SX-1) 10.5g, and sterile water is added to be settled to
1000ml takes 1mL to be divided in 1.5mL centrifuge tube with the manual controlled variable pipettor of 1ml single track, and every effective tap water of centrifugation is fixed
Hold to 13L, as changes and kill agent SX-1 foliar spray pharmacy solution.
(2) change the preparation for killing agent SX-1 stem brush medicament: agent SX-1 first is killed into change and is configured to 100mg/L concentration mother liquor, takes mother
Liquid 5.5ml is settled to 100ml, as stem brush finish liquid with edible oil.
(3) change the preparation for killing agent SX-1 note stem medicament: change kills agent SX-1 and is configured to concentration 100mg/L with sterile water, takes 1mL
It is divided in the centrifuge tube of 1.5ml, as note stem liquid.
Application Example two:
Rape kills tri- kinds of application methods of agent SX-1 to No. 10 Qin's oil 88, Qin You seven and Qin You male sterility induction effects
Fruit, phytotoxicity and sterile duration.In the high 20~25cm of rape a kind of sedge, maximum bud length about 2mm or so, pollen mother cell four divides
Body phase to monokaryotic stage is respectively adopted: 1. foliar spray method: foliage-spray 5.5mg/L, 2.5ml/ plant dosage；2. stem brush method: using hair
Dipping stem finish is coated in rape stem away from rhizome 10cm or so place, and ring stem one encloses, Tu Kuan 5cm or so, every plant of painting 0.2ml
Left and right；3. infusing stem method: use small wood cone point downward 45 degree of piercings caulome at 10~15cm of rape stem, the depth of piercing with
It does not pierce through subject to rape stem, recycles pipettor that 25ul, the change that concentration is 100mg/L are killed agent SX-1 aqueous solution and be injected into oil
In the small holes that dish stalk is pierced.
The result shows that: change to kill tri- kinds of application methods of agent SX-1 and can induce three rape varieties and reaches relatively high male not
Level is educated, sterile plant rate reaches 93.79%~100% (table one), wherein note stem method male sterility inducing effect is best, three product
Kind complete sterility strain rate, sterile plant rate reach 100%, and semisterility strain rate, normal strain rate and phytotoxicity strain rate are 0, sterile holding effect
21~24d of phase almost continues the entire florescence.Although spray-on process and stem brush method, which also can induce rape, reaches higher level male not
Educate, but its semisterility strain rate (3.33%~5.71%), normal strain rate (2.50%~6.21%) and phytotoxicity strain rate (8.28%~
12.5%) higher, sterile 15~17d of duration.
The change of table 1 is killed agent SX-1 difference application method induction rapeseed male sterility effect and is compared
Application Example three:
Rape kills tri- kinds of application methods of agent SX-1 to Qin's oil 88, No. 10 agronomy of Qin You seven and Qin You and yield traits
It influences.
The result shows that: with three kinds of application method induced male infertility to Qin's oil 88, No. 10 agronomy of Qin You seven and Qin You and
Yield traits will cause certain influence (table 2).Wherein spray-on process is affected, and three oil can be made in spontaneous pollination
Vegetable kind plant height, effective branch position, main inflorescence length, main inflorescence silique number, seed number per pod and single plant yield significantly reduce.Stem brush method
Influence to rape is smaller, the plant heights of three kinds of rapes, main inflorescence length, main inflorescence silique number, seed number per pod and single plant yield and comparison
According to decreasing but not significant, and infuse that influence of the stem method to three kinds of rapes is minimum, and agronomy and yield traits and control are without obvious
Difference.
Influence of the agent SX-1 difference application method induced male infertility to rape is killed in the change of table 2
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (3)
1. a kind of rape chemical hybridizing agent accuracy pesticide applying method, which is characterized in that rape chemical hybridizing agent accuracy pesticide applying side
Method utilizes a root long 10cm, the small wood that the front end diameter 0.55cm is cone；The high 1.5cm of cone, in the high 25cm of rape a kind of sedge,
Bud 2mm period downward 45 degree of piercings caulome at rape stem 10cm~15cm with small wood cone point；The depth of piercing with
It does not pierce through subject to rape stem；Recycle pipettor that 25ul, the change that concentration is 100mg/L are killed agent SX-1 aqueous solution and be injected into oil
In the small holes that dish stalk is pierced；
The cone of high 1.5cm is processed into using turning pencil knife for the cone in the small wood of cone in the front end；
Describedization kills agent SX-1 aqueous solution and is configured to concentration 100mg/L with sterile water；
The pipettor uses 20ul~100ul single channel manual controlled variable pipettor.
2. a kind of carry out rape indirect labor emasculation hybridization using rape chemical hybridizing agent accuracy pesticide applying method described in claim 1
The method of breeding.
3. a kind of kill hybridization examination using the progress of rape chemical hybridizing agent accuracy pesticide applying method described in claim 1 rape small area
The method for testing the production of hybrid seeds.
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| CN201611176757.1A CN106577263B (en) | 2016-12-19 | 2016-12-19 | A kind of rape chemical hybridizing agent accuracy pesticide applying method |
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| CN201611176757.1A CN106577263B (en) | 2016-12-19 | 2016-12-19 | A kind of rape chemical hybridizing agent accuracy pesticide applying method |
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