CN106561204A - Method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo - Google Patents
Method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo Download PDFInfo
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- CN106561204A CN106561204A CN201610902271.5A CN201610902271A CN106561204A CN 106561204 A CN106561204 A CN 106561204A CN 201610902271 A CN201610902271 A CN 201610902271A CN 106561204 A CN106561204 A CN 106561204A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G25/00—Watering gardens, fields, sports grounds or the like
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Abstract
The invention discloses a method for improving alcohol-soluble extractive content of dendrobium officinale kimura et migo. The method is mainly composed of the following steps of kind selecting, taking seedlings out of bottoms, performing disinfection and air drying processing, transplanting, performing seedling period management, performing seasonal management, performing sun shading and ventilation, harvesting stems and performing low-temperature processing. According to the method of the invention, authorized excellent new species are utilized for performing facility cultivation, and management of links such as tissue culture seedling transplanting, water and fertilizer management, disease and insect control, stem plucking and low-temperature processing are reinforced. The method can ensure a fact that the produced dendrobium officinale kimura et migo has a polysaccharide content that satisfies a national standard and furthermore settles a problem that the alcohol-soluble extractive content of dendrobium officinale kimura et migo fresh branches which are supplied on market cannot reach the national standard for a long time. The alcohol-soluble extractive content is remarkably improved and can reach 10-15% and is remarkably higher than 6.5% specified in the national standard.
Description
Technical field
The invention belongs to the plant regeneration technology by tissue culture technique in technical field of Chinese medicines, and in particular to a kind of
The method for improving Herba Dendrobii ethanol-soluble extractiveses content.
Background technology
Herba Dendrobii (Dendrobium) is orchid family Dendrobium Epiphyte, is exactly since ancient times generally acknowledged famous and precious medium-height grass
Medicine.Environmental condition needed for dendrobium officinale growth promoter is extremely harsh, and poor growth, breeding potential is extremely low, and artificially robs
Property excavation be directed at dendrobium officinale resource and be bordering on exhaustion.The many reports of current Herba Dendrobii artificial culture, but it is general
Store-through is not up to the requirement problem of national standard 6.5% in Herba Dendrobii ethanol-soluble extractiveses content, so as to govern iron sheet stone
The fast development of dry measure used in former times industry.
The patent of invention of Publication No. CN105748944A, entitled " a kind of preparation method of Herba Dendrobii decoction pieces ", leads to
A series of step is crossed, i.e.,：Pre-treatment：To naturally dry after the fresh bar cleaning of Herba Dendrobii；Section：Oblique thin slice is cut into, it is described
The angle of section is 15～30 °, and the thickness of described oblique thin slice is 2～6mm；Cold drying：Oblique thin slice is laid in into bottom saturating
In the container of gas, it is dried under 15～25 DEG C of ventilated environments, and interval is stirred, and obtains Herba Dendrobii piece 1；Middle temperature is dried：Will
Herba Dendrobii piece 1 is transferred in pallet, is dried in 35～60 DEG C of aeration cabinets, obtains Herba Dendrobii piece 2；Secondary low temperature is done
It is dry：Herba Dendrobii piece 2 is laid in again in the ventilative container in bottom, is dried under 20～25 DEG C of ventilated environments, until iron sheet stone
The moisture of dry measure used in former times reaches less than 10%, and the ethanol-soluble extractiveses content that can reach obtained Herba Dendrobii decoction pieces reaches
More than 7.5% target.But this method belongs to Chinese crude drug processing technique field, it is impossible in the technical field of cultivation of Herba Dendrobii
Find application.In addition, Publication No. CN101564008A, it is entitled " a kind of dendrobium candidum axenic seedlings without hormone culture and fast
The domestic patent of invention of fast propagation method " also disclose that a kind of dendrobium candidum axenic seedlings without hormone culture and quick breeding side
Method, the axenic germination of content including Seeds of Dendrobium Candidum, the formation of protocorm and seedling with preserve, aseptic seedling is taken root, breeds
And strong seedling culture, the process such as the experienced Seedling of aseptic seedling and transplanting.But the technology has the drawback that the induction of protocorm and seedling
Journey is related to the use of HONGGUANG, green glow, unglazed, gold-tinted and blue light, therefore process is extremely complex, and the process of induction also is difficult to control,
It is unfavorable in large-scale industrial production application.And the method does not solve Herba Dendrobii ethanol-soluble extractiveses content and does not reach
Target problem.
The content of the invention
The technical problem to be solved in the present invention is that the Herba Dendrobii alcohol-soluble that existing officinal dendrobium stem plantation technology is present is leached
Thing content is low, does not reach the problem of national standard 6.5%.
In order to solve the above problems, the present invention proposes a kind of method for improving Herba Dendrobii ethanol-soluble extractiveses content.Profit
Carry out facility cultivation with the improved Varieties of authorization, strengthen tissue culture transplantation of seedlings, water and fertilizer management, crop protection, stem bar pluck, it is low
Temperature processes each link management, it is ensured that not only polyoses content is up to state standards the Herba Dendrobii produced, and solves long-term
Since the ethanol-soluble extractiveses content of the fresh bar of Herba Dendrobii supplied on market do not reach the quality problems of national standard, its alcohol is molten
Property extract content be significantly improved, up to 13%-18%, hence it is evident that higher than the 6.5% of national standard.Technology is unique and simple
Single material benefit, using value is high, can promote the sustainable development of Herba Dendrobii industry.
Technical scheme is as follows：
A kind of method for improving Herba Dendrobii ethanol-soluble extractiveses content, comprises the steps of：
(1) Fruit variety, seedling bottle outlet, sterilize and dry process：High-quality Herba Dendrobii bottle seedling is selected, through the refining of 10 days
After seedling bottle outlet is colonized, and the ambient temperature of seedling bottle outlet is 15-35 degree Celsius, and every clump of individual plant of bottle seedling is higher than 3 centimetres, has 4-6 to save, and 4
Piece is with blade, and the root of more than 3 is the culture medium rinsed clean of Seedling root after seedling bottle outlet then molten for gloomy manganese -with 500 times
Liquid disinfectant 15 minutes, dries under diffuse light naturally, and seedling root gradually softens and become white after 5 to 7 hours；
(2) transplant：Transplant before to substrate with Dai Sen Meng -500 times of liquid carry out spraying disinfection, sterilize, dug Seedling cave, Seedling cave
3 to 5 centimetres of depth, is then put into Seedling root in Seedling cave, covers substrate, is disappeared with 1000 times of solution foliage-sprays of carbendazim
Poison, after transplanting 3 days 500 times of plant-mineral-elements are sprayed；
(3) seedling period management：
It is divided into 3 stages：
1st stage is 7 to 15 days after transplanting, and the sunshade net of lid 90%, air humidity is maintained at 85%-90%, waters
Water quality requirement pH value is 5-7；
2nd stage was 15～30 days after transplanting, and the sunshade net of lid 70 to 80%, air humidity is maintained at 70%-80%, applies
Slow-release fertilizer, 6 months slow release phases, Main Ingredients and Appearance is 1:1:1 nitrogen, phosphorus, potassium；
3rd stage be transplant 30 days after, 1500 times of foliage-spray compound fertilizer 1 time weekly, the sunshade net of lid 50 to 70% is empty
Air humidity degree is maintained at 70%-80%；
(4) season management：Carry out corresponding management according to the season in summer, autumn, winter and spring respectively；
(5) stem harvesting：Harvest before the Beginning of Winter to Clear and Bright, harvest front ethanol and harvesting instrument is carried out disinfection；
(6) K cryogenic treatment：Room temperature is dried after the Herba Dendrobii stem band leaf cleaning of harvesting, 4-6 DEG C of cryopreservation 10-20 days.
Further, above-mentioned season management divides four seasons to implement, and the management process in each season is as follows：
1, summer management：
A, sunshade and ventilation：Using double-layered sunshade net, sunshade reticular density more than 80%, when midsummer temperature is up to 30-35 DEG C
When, forced ventilation is carried out when humidity reaches more than 90%；
B, water：One layer of lichen being covered in stromal surface and reducing moisture evaporation, cover 2-4 centimetre of moss depth, humid control exists
70～90%, when midsummer temperature is up to 30-35 DEG C, spray once per hour, while plus forced ventilation；
C, fertilising：1500 times of N P and Ks 1 of foliage-spray weekly：1：1 compound fertilizer's solution 1 time；
D, disease control：Apply 1000 times of liquid of thiophanate methyl to be prevented and treated, spray 2 to 3 times, per 20 days left sides of minor tick
It is right；
2, autumn management：
A, sunshade and ventilation：Double-layered sunshade net, at 3 points in afternoon are used when having sunburst before at 10 points in the morning at 3 points in afternoon
Monolayer sunshade net is used later, and daily ventilation principle is that humidity is divulged information more than 60% when temperature is more than 20 degrees Celsius；
B, water：Foliar spray waters, and air humidity is controlled 40%～60%；
C, fertilising：1500 times of N P and Ks 1 were sprayed per 20 days：1：1 compound fertilizer's solution 1 time；
3, winter management：
A, insulation：Temperature is maintained between 15 DEG C to 20 DEG C；
B, leaf and stromal surface water spray, is defined by squirting substrate epidermis, leaf surfaces, humid control 20%～
40%；
4, spring management：
A, sunshade and ventilation：Using monolayer internal sunshade net sunshade, before at 10 points in the morning at 3 points in afternoon, using 50% to 70%
Sunshade net sunshade, temperature more than 20 degrees Celsius, more than 60% divulge information by humidity；
B, water：A water is sprayed to leaf with stromal surface within 2～4 days, be defined by squirting stromal surface, air humidity control
40%～70%；
C, fertilising：1500 times of N P and Ks 1 are sprayed at the beginning of spring：1：1 compound fertilizer's solution 1 time, Chun Moshi nitrogen phosphorus potassium slow releases fertilizer is once.
In view of the difference of the weather conditions of various places, the concrete month in the four seasons and length have some differences, can combine
Actual situation is adjusted.In general, in officinal dendrobium stem plantation management, above-mentioned summer is usually 4 to June, autumn
For 7 to September part, winter is 10 to December, and spring is 1 to the March in the coming year.
Beneficial effects of the present invention：
1, the present invention carries out facility cultivation using the improved Varieties of authorization, strengthens tissue culture transplantation of seedlings, water and fertilizer management, disease
Worm preventing and treating, stem bar are plucked, each link management of K cryogenic treatment, it is ensured that not only polyoses content reaches country to the Herba Dendrobii produced
Standard, and the ethanol-soluble extractiveses content of the fresh bar of Herba Dendrobii for solving to be supplied on market for a long time does not reach national standard
Quality problems, its ethanol-soluble extractiveses content is significantly improved, up to 10%-15%, hence it is evident that higher than national standard
6.5%.
2, the enforcement of the present invention is carried out by need to only having simple cultivation booth and stored frozen equipment, with operation letter
The high advantage of single, easy realization, using value.
Description of the drawings
Fig. 1 is the schematic flow sheet of the present invention.
Specific embodiment
The specific embodiment of the present invention is further described in conjunction with accompanying drawing.
As shown in figure 1, method proposed by the present invention is mainly made up of following step, i.e., Fruit variety, seedling bottle outlet,
Sterilization and processs is dried, transplanted, seedling period management, season management, sunshade and ventilation, stem is harvested and K cryogenic treatment.Now by real
Apply example to be described in further detail the above of the present invention again.
It should be noted that in view of the difference of various places weather conditions during cultivation, the concrete month in the four seasons and length meeting
There are some differences, can be adjusted with reference to actual situation.In general, in officinal dendrobium stem plantation management, summer is usual
For 4 to June, autumn is 7 to September part, and winter is 10 to December, spring for the coming year 1 to March.
Embodiment 1
A kind of method for improving Herba Dendrobii ethanol-soluble extractiveses content：
(1) Fruit variety and seedling bottle outlet and sterilization, dry process：Section is passed through from all No. 2 Herba Dendrobii bottle seedlings in selection
Bottle outlet field planting after the seedling exercising of 10 days, the ambient temperature of seedling bottle outlet is 15-35 degree Celsius, and every clump of individual plant of bottle seedling is higher than 3 centimetres, is had
4-6 is saved, 4 with blade, the root of more than 3, the culture medium rinsed clean of Seedling root after seedling bottle outlet, then with 500 times of generations
Sen Meng -solution disinfection 15 minutes, dry naturally under diffuse light, seedling root gradually softens and becomes white after 5 to 7 hours；
(2) transplant：Transplant before to substrate with Dai Sen Meng -500 times of liquid carry out spraying disinfection, sterilize, dug Seedling cave, Seedling cave
3 to 5 centimetres of depth, is then put into Seedling root in Seedling cave, covers substrate, is disappeared with 1000 times of solution foliage-sprays of carbendazim
Poison, after transplanting 3 days 500 times of plant-mineral-elements are sprayed；
(3) seedling period management：It is divided into 3 stages, first stage is 7 to 15 days after transplanting, and the sunshade net of lid 90% is empty
Air humidity degree is maintained at 85%, the water quality requirement pH value that waters be 5-7, second stage be transplant after 15～30 days, lid 70 to 80%
Sunshade net, air humidity is maintained at 70%, applies a slow-release fertilizer, 6 months slow release phases, Main Ingredients and Appearance be 1 to 1 nitrogen than 1, phosphorus,
Potassium, the phase III be transplant 30 days after, 1500 times of foliage-spray compound fertilizer 1 time weekly, the sunshade net of lid 50 to 70%, air is wet
Degree is maintained at 80%；
(4) season management：4.1 summer management：In officinal dendrobium stem plantation management, 4 to June is summer management, A, screening
Sun and ventilation：Using double-layered sunshade net, sunshade reticular density more than 80%, when midsummer, temperature was up to 30-35 DEG C, humidity reaches
To carry out forced ventilation when more than 90%, B, water：One layer of lichen is covered in stromal surface and reduce moisture evaporation, cover moss depth 2-
4 centimetres, humid control when midsummer temperature is up to 30-35 DEG C, is sprayed once per hour 70%, while plus forced ventilation；C, apply
Fertilizer：1500 times of N P and Ks 1 of foliage-spray weekly：1：1 compound fertilizer's solution 1 time, D, disease control：Apply 1000 times of thiophanate methyl
Liquid was prevented and treated, and is sprayed 2 to 3 times, per minor tick 20 days or so；4.2 autumns managed：7 to September part after summer is autumn pipe
Reason, A, sunshade and ventilation：Double-layered sunshade net is used when having sunburst before at 10 points in the morning at 3 points in afternoon, is made after at 3 points in afternoon
Use monolayer sunshade net, daily ventilation principle is that humidity is divulged information more than 60% when temperature is more than 20 degrees Celsius, B, water：
Foliar spray waters, and air humidity is controlled in 60%, C, fertilising：1500 times of N P and Ks 1 were sprayed per 20 days：1：1 compound fertilizer's solution 1
It is secondary, 4.3 winter managements：10 to December is winter management, A, insulation：Temperature is maintained between 15 DEG C to 20 DEG C, B, leaf with
Stromal surface is sprayed water, and is defined by squirting substrate epidermis, leaf surfaces, and 20%, 4.4 spring managed humid control, Herba Dendrobii
In cultivation management, 1 to March is to manage in spring, A, sunshade and ventilation：Using monolayer internal sunshade net sunshade, at 10 points in the morning is under
Before 3 points of noon, using 50% to 70% sunshade net sunshade, temperature more than 20 degrees Celsius, more than 60% divulge information by humidity, B, pours
Water：A water being sprayed to leaf with stromal surface within 2～4 days, being defined by squirting stromal surface, air humidity is controlled in 70%, C, applied
Fertilizer：1500 times of N P and Ks 1 are sprayed at the beginning of spring：1：1 compound fertilizer's solution 1 time, Chun Moshi nitrogen phosphorus potassium slow releases fertilizer is once；
(5) stem harvesting：Harvest before the Beginning of Winter to Clear and Bright, harvest front ethanol and harvesting instrument is carried out disinfection；
(6) K cryogenic treatment：Room temperature is dried after the Herba Dendrobii stem band leaf cleaning of harvesting, and 4-6 DEG C of cryopreservation is preserved 10 days.
Embodiment 2
A kind of method for improving Herba Dendrobii ethanol-soluble extractiveses content,
(1) Fruit variety and seedling bottle outlet and sterilization, dry process：Section is passed through from all No. 2 Herba Dendrobii bottle seedlings in selection
Bottle outlet field planting after the seedling exercising of 10 days, the ambient temperature of seedling bottle outlet is 15-35 degree Celsius, and every clump of individual plant of bottle seedling is higher than 3 centimetres, is had
4-6 is saved, 4 with blade, the root of more than 3, the culture medium rinsed clean of Seedling root after seedling bottle outlet, then with 500 times of generations
Sen Meng -solution disinfection 15 minutes, dry naturally under diffuse light, seedling root gradually softens and becomes white after 5 to 7 hours；
(2) transplant：Transplant before to substrate with Dai Sen Meng -500 times of liquid carry out spraying disinfection, sterilize, dug Seedling cave, Seedling cave
3 to 5 centimetres of depth, is then put into Seedling root in Seedling cave, covers substrate, is disappeared with 1000 times of solution foliage-sprays of carbendazim
Poison, after transplanting 3 days 500 times of plant-mineral-elements are sprayed；
(3) seedling period management：It is divided into 3 stages, first stage is 7 to 15 days after transplanting, and the sunshade net of lid 90% is empty
Air humidity degree is maintained at 90%, the water quality requirement pH value that waters be 5-7, second stage be transplant after 15～30 days, lid 70 to 80%
Sunshade net, air humidity is maintained at 70%, applies a slow-release fertilizer, 6 months slow release phases, Main Ingredients and Appearance be 1 to 1 nitrogen than 1, phosphorus,
Potassium, the phase III be transplant 30 days after, 1500 times of foliage-spray compound fertilizer 1 time weekly, the sunshade net of lid 50 to 70%, air is wet
Degree is maintained at 80%；
(4) season management：4.1 summer management：In officinal dendrobium stem plantation management, 4 to June is summer management, A, screening
Sun and ventilation：Using double-layered sunshade net, sunshade reticular density more than 80%, when midsummer, temperature was up to 30-35 DEG C, humidity reaches
To carry out forced ventilation when more than 90%, B, water：One layer of lichen is covered in stromal surface and reduce moisture evaporation, cover moss depth 2-
4 centimetres, humid control when midsummer temperature is up to 30-35 DEG C, is sprayed once per hour 90%, while plus forced ventilation；C, apply
Fertilizer：1500 times of N P and Ks 1 of foliage-spray weekly：1：1 compound fertilizer's solution 1 time, D, disease control：Apply 1000 times of thiophanate methyl
Liquid was prevented and treated, and is sprayed 2 to 3 times, per minor tick 20 days or so；4.2 autumns managed：7 to September part after summer is autumn pipe
Reason, A, sunshade and ventilation：Double-layered sunshade net is used when having sunburst before at 10 points in the morning at 3 points in afternoon, is made after at 3 points in afternoon
Use monolayer sunshade net, daily ventilation principle is that humidity is divulged information more than 60% when temperature is more than 20 degrees Celsius, B, water：
Foliar spray waters, and air humidity is controlled in 40%, C, fertilising：1500 times of N P and Ks 1 were sprayed per 20 days：1：1 compound fertilizer's solution 1
It is secondary, 4.3 winter managements：10 to December is winter management, A, insulation：Temperature is maintained between 15 DEG C to 20 DEG C, B, leaf with
Stromal surface is sprayed water, and is defined by squirting substrate epidermis, leaf surfaces, and 40%, 4.4 spring managed humid control, Herba Dendrobii
In cultivation management, 1 to March is to manage in spring, A, sunshade and ventilation：Using monolayer internal sunshade net sunshade, at 10 points in the morning is under
Before 3 points of noon, using 50% to 70% sunshade net sunshade, temperature more than 20 degrees Celsius, more than 60% divulge information by humidity, B, pours
Water：A water being sprayed to leaf with stromal surface within 2～4 days, being defined by squirting stromal surface, air humidity is controlled in 40%, C, applied
Fertilizer：1500 times of N P and Ks 1 are sprayed at the beginning of spring：1：1 compound fertilizer's solution 1 time, Chun Moshi nitrogen phosphorus potassium slow releases fertilizer is once；
(5) stem harvesting：Harvest before the Beginning of Winter to Clear and Bright, harvest front ethanol and harvesting instrument is carried out disinfection；
(6) K cryogenic treatment：Room temperature is dried after the Herba Dendrobii stem band leaf cleaning of harvesting, and 4-6 DEG C of cryopreservation is preserved 20 days.
Embodiment 3
A kind of method for improving Herba Dendrobii ethanol-soluble extractiveses content,
(1) Fruit variety and seedling bottle outlet and sterilization, dry process：Section is passed through from all No. 2 Herba Dendrobii bottle seedlings in selection
Bottle outlet field planting after the seedling exercising of 10 days, the ambient temperature of seedling bottle outlet is 15-35 degree Celsius, and every clump of individual plant of bottle seedling is higher than 3 centimetres, is had
4-6 is saved, 4 with blade, the root of more than 3, the culture medium rinsed clean of Seedling root after seedling bottle outlet, then with 500 times of generations
Sen Meng -solution disinfection 15 minutes, dry naturally under diffuse light, seedling root gradually softens and becomes white after 5 to 7 hours；
(2) transplant：Transplant before to substrate with Dai Sen Meng -500 times of liquid carry out spraying disinfection, sterilize, dug Seedling cave, Seedling cave
3 to 5 centimetres of depth, is then put into Seedling root in Seedling cave, covers substrate, is disappeared with 1000 times of solution foliage-sprays of carbendazim
Poison, after transplanting 3 days 500 times of plant-mineral-elements are sprayed；
(3) seedling period management：It is divided into 3 stages, first stage is 7 to 15 days after transplanting, and the sunshade net of lid 90% is empty
Air humidity degree is maintained at 88%, the water quality requirement pH value that waters be 5-7, second stage be transplant after 15～30 days, lid 70 to 80%
Sunshade net, air humidity is maintained at 75%, applies a slow-release fertilizer, 6 months slow release phases, Main Ingredients and Appearance be 1 to 1 nitrogen than 1, phosphorus,
Potassium, the phase III be transplant 30 days after, 1500 times of foliage-spray compound fertilizer 1 time weekly, the sunshade net of lid 50 to 70%, air is wet
Degree is maintained at 75%；
(4) season management：4.1 summer management：In officinal dendrobium stem plantation management, 4 to June is summer management, A, screening
Sun and ventilation：Using double-layered sunshade net, sunshade reticular density more than 80%, when midsummer, temperature was up to 30-35 DEG C, humidity reaches
To carry out forced ventilation when more than 90%, B, water：One layer of lichen is covered in stromal surface and reduce moisture evaporation, cover moss depth 2-
4 centimetres, humid control when midsummer temperature is up to 30-35 DEG C, is sprayed once per hour 80%, while plus forced ventilation；C, apply
Fertilizer：1500 times of N P and Ks 1 of foliage-spray weekly：1：1 compound fertilizer's solution 1 time, D, disease control：Apply 1000 times of thiophanate methyl
Liquid was prevented and treated, and is sprayed 2 to 3 times, per minor tick 20 days or so；4.2 autumns managed：7 to September part after summer is autumn pipe
Reason, A, sunshade and ventilation：Double-layered sunshade net is used when having sunburst before at 10 points in the morning at 3 points in afternoon, is made after at 3 points in afternoon
Use monolayer sunshade net, daily ventilation principle is that humidity is divulged information more than 60% when temperature is more than 20 degrees Celsius, B, water：
Foliar spray waters, and air humidity is controlled in 50%, C, fertilising：1500 times of N P and Ks 1 were sprayed per 20 days：1：1 compound fertilizer's solution 1
It is secondary, 4.3 winter managements：10 to December is winter management, A, insulation：Temperature is maintained between 15 DEG C to 20 DEG C, B, leaf with
Stromal surface is sprayed water, and is defined by squirting substrate epidermis, leaf surfaces, and 30%, 4.4 spring managed humid control, Herba Dendrobii
In cultivation management, 1 to March is to manage in spring, A, sunshade and ventilation：Using monolayer internal sunshade net sunshade, at 10 points in the morning is under
Before 3 points of noon, using 50% to 70% sunshade net sunshade, temperature more than 20 degrees Celsius, more than 60% divulge information by humidity, B, pours
Water：A water being sprayed to leaf with stromal surface within 2～4 days, being defined by squirting stromal surface, air humidity is controlled in 60%, C, applied
Fertilizer：1500 times of N P and Ks 1 are sprayed at the beginning of spring：1：1 compound fertilizer's solution 1 time, Chun Moshi nitrogen phosphorus potassium slow releases fertilizer is once；
(5) stem harvesting：Harvest before the Beginning of Winter to Clear and Bright, harvest front ethanol and harvesting instrument is carried out disinfection；
(6) K cryogenic treatment：Room temperature is dried after the Herba Dendrobii stem band leaf cleaning of harvesting, and 4-6 DEG C of cryopreservation is preserved 15 days.
Embodiment 4：Ethanol-soluble extractiveses assay, specifically comprises the steps of：
1st, the fresh bar of Herba Dendrobii supplied on market is bought.
2nd, take above-described embodiment 1 preparation Herba Dendrobii and market on supply the fresh bar of Herba Dendrobii, 1000g is taken respectively,
85% alcohol reflux is used, is extracted 2 hours, alcohol adding amount is 10 times of amounts of medical material weight, obtains ethanol-soluble extractiveses, and drying claims
Weight, Herba Dendrobii gained ethanol-soluble extractiveses prepared by embodiment 1 are the 14% of medical material weight, the Herba Dendrobii supplied on market
Gained ethanol-soluble extractiveses are the 3.8% of medical material weight, illustrate that ethanol-soluble extractiveses content of the present invention is significantly improved.
The preferably middle section of above-mentioned seedling is entitled from all No. 2 Herba Dendrobii Latins：Dendrobium officinale"Zhongke
Congdu 2 Hao"。
It should be noted that above-described embodiment provided by the present invention only has schematically, not with the restriction present invention's
The effect of the scope being embodied as.Protection scope of the present invention should for the person of ordinary skill of the art show including those
And the conversion that is clear to or replacement scheme.
Claims (3)
1. it is a kind of improve Herba Dendrobii ethanol-soluble extractiveses content method, it is characterised in that comprise the steps of：
(1) Fruit variety, seedling bottle outlet, sterilize and dry process：High-quality Herba Dendrobii bottle seedling is selected, after the seedling exercising of 10 days
Bottle outlet is colonized, and the ambient temperature of seedling bottle outlet is 15-35 degree Celsius, and every clump of individual plant of bottle seedling is higher than 3 centimetres, has 4-6 to save, 4 with
Blade, the root of more than 3, the culture medium rinsed clean of Seedling root after seedling bottle outlet, then with 500 times of Dai Sen Meng -solution disappear
Poison 15 minutes, dries under diffuse light naturally, and seedling root gradually softens and become white after 5 to 7 hours；
(2) transplant：Transplant before to substrate with Dai Sen Meng -500 times of liquid carry out spraying disinfection, sterilize, dug Seedling cave, Seedling acupoint depth 3
To 5 centimetres, then Seedling root is put in Seedling cave, covers substrate, carried out disinfection with 1000 times of solution foliage-sprays of carbendazim, transplanted
500 times of plant-mineral-elements are sprayed after 3 days；
(3) seedling period management：
It is divided into 3 stages：
1st stage is 7 to 15 days after transplanting, and the sunshade net of lid 90%, air humidity is maintained at 85%-90%, water quality of watering
Requirement pH value is 5-7；
2nd stage was 15～30 days after transplanting, and the sunshade net of lid 70 to 80%, air humidity is maintained at 70%-80%, applies once
Slow-release fertilizer, 6 months slow release phases, Main Ingredients and Appearance is 1:1:1 nitrogen, phosphorus, potassium；
3rd stage be transplant 30 days after, 1500 times of foliage-spray compound fertilizer 1 time weekly, the sunshade net of lid 50 to 70%, air is wet
Degree is maintained at 70%-80%；
(4) season management：Carry out corresponding management according to the season in summer, autumn, winter and spring respectively；
(5) stem harvesting：Harvest before the Beginning of Winter to Clear and Bright, harvest front ethanol and harvesting instrument is carried out disinfection；
(6) K cryogenic treatment：Room temperature is dried after the Herba Dendrobii stem band leaf cleaning of harvesting, 4-6 DEG C of cryopreservation 10-20 days.
2. it is according to claim 1 it is a kind of improve Herba Dendrobii ethanol-soluble extractiveses content method, it is characterised in that institute
State season as follows the step of manage：
2.1 summer management：
A, sunshade and ventilation：Using double-layered sunshade net, sunshade reticular density more than 80% is wet when midsummer, temperature was up to 30-35 DEG C
Degree will carry out forced ventilation when reaching more than 90%；
B, water：Cover one layer of lichen in stromal surface and reduce moisture evaporation, cover 2-4 centimetre of moss depth, humid control 70～
90%, when midsummer temperature is up to 30-35 DEG C, spray once per hour, while plus forced ventilation；
C, fertilising：1500 times of N P and Ks 1 of foliage-spray weekly：1：1 compound fertilizer's solution 1 time；
D, disease control：Apply 1000 times of liquid of thiophanate methyl to be prevented and treated, spray 2 to 3 times, per minor tick 20 days or so；
2.2 autumns managed：
A, sunshade and ventilation：Double-layered sunshade net is used when having sunburst before at 10 points in the morning at 3 points in afternoon, after at 3 points in afternoon
Using monolayer sunshade net, daily ventilation principle is that humidity is divulged information more than 60% when temperature is more than 20 degrees Celsius；
B, water：Foliar spray waters, and air humidity is controlled 40%～60%；
C, fertilising：1500 times of N P and Ks 1 were sprayed per 20 days：1：1 compound fertilizer's solution 1 time；
2.3 winter management：
A, insulation：Temperature is maintained between 15 DEG C to 20 DEG C；
B, leaf and stromal surface water spray, is defined by squirting substrate epidermis, leaf surfaces, and humid control is 20%～40%；
2.4 spring managed：
A, sunshade and ventilation：Using monolayer internal sunshade net sunshade, before at 10 points in the morning at 3 points in afternoon, using 50% to 70% sunshade
Net sunshade, temperature more than 20 degrees Celsius, more than 60% divulge information by humidity；
B, water：A water being sprayed to leaf with stromal surface within 2～4 days, being defined by squirting stromal surface, air humidity control exists
40%～70%；
C, fertilising：1500 times of N P and Ks 1 are sprayed at the beginning of spring：1：1 compound fertilizer's solution 1 time, Chun Moshi nitrogen phosphorus potassium slow releases fertilizer is once.
3. it is according to claim 2 it is a kind of improve Herba Dendrobii ethanol-soluble extractiveses content method, it is characterised in that
In officinal dendrobium stem plantation management, the summer is usually 4 to June, and autumn is 7 to September part, and winter is 10 to December, the spring
Season is 1 to the March in the coming year.
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