CN106552262A - A kind of deer collagen peptide liposome with function of blood sugar reduction and its preparation and application - Google Patents
A kind of deer collagen peptide liposome with function of blood sugar reduction and its preparation and application Download PDFInfo
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- CN106552262A CN106552262A CN201510621656.XA CN201510621656A CN106552262A CN 106552262 A CN106552262 A CN 106552262A CN 201510621656 A CN201510621656 A CN 201510621656A CN 106552262 A CN106552262 A CN 106552262A
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Abstract
The present invention relates to deer collagen peptide liposome, specifically a kind of to have blood sugar lowering deer collagen peptide liposome and prepare and apply.Prepared by deer collagen peptide become liposome, with to suppress function of blood sugar reduction of the dipeptidylpeptidase activity as target.Deer collagen peptide liposome prepared by the present invention can make deer collagen peptide from the degraded of digestive enzyme, and the target organ that can go directly plays dipeptidyl peptidase enzyme inhibition activity, so as to reach hypoglycemic purpose.The present invention solve deer collagen peptide it is oral when easily by the deactivated difficult problem of digestive tract enzymatic degradation, improve the oral bioavailability of deer collagen peptide, expand deer collagen peptide takes mode, can be used for the fields such as medicine, functional food, food additive, with broad prospect of application.
Description
Technical field
The present invention relates to deer collagen peptide, specifically a kind of deer collagen with function of blood sugar reduction
Peptidoliposome and its preparation and application.
Background technology
DPP IV (dipeptidyl peptidase-IV DPP-IV) is (EC3.4.14.5)
It is a kind of serine protease, is distributed widely in human body.DPP-IV is by cutting to polypeptide
Cutting inactivates which, so as to reach the effect of regulation of physiological functions.DPP-IV selective hydrolysises N- is last
There is on the second of end the protein of proline (Pro) or alanine (Ala).Its substrate specificity
Including：Glucagon-like-peptide-1 (GLP217-36), Gastric inhibitory polypeptide (GIP1-42), nerve
Peptide (NPY), YY peptides (PYY) and pancreatic polypeptide family (PP-family) etc..These materials
Common feature is exactly the deputy amino acid residue proline in N- ends or the third ammonia
Sour residue.DPP-IV by act on above peptide substrate and diabetes, glucose tolerance,
Obesity, appetite stimulator, hyperlipidemia, osteoporosises, neuropeptide metabolism and T- cells swash
Play an important role in the relevant disease such as living.Therefore, giving DPP-IV inhibitor in vivo can
The N- terminal degradations of prevention related substrates peptide, so as to ensure that function of human body is normally run.
Complete GLP-1 and GIP reactions are cut off N- ends 2 amino acid residue by DPP-IV
Afterwards so as to which inactivation is so as to physiological reactions such as the blood glucose regulations that affects GLP-1 related to GIP.
The N- terminal degradations that DPP-IV inhibitor can prevent GLP-1 and GIP are given in vivo, make pancreas
Island element secretions increase so as to improve glucose tolerance.In view of DPP-IV inhibitor and blood glucose generation
The substantial connection thanked, DPP-IV inhibitor have become the research heat of new type 2 diabetes mellitus medicine
Point.
DPP-IV inhibitor is carried by affecting sensitivity and β cells of the insulin to glucose
Sensitivity of the high surrounding tissue to insulin, promote Chylomicron and very low density lipoprotein (VLDL) point
Solution, so as to play a part of lipid-loweringing.Based on it is same the reasons why, DPP-IV also simultaneously has and subtracts
The functions such as fertilizer.Therefore have broad application prospects.
Deer collagen peptide has dipeptidyl peptidase enzyme inhibition activity, is applied to medicine, health product, food
The fields such as product play function of blood sugar reduction, but as other bioactive peptide problem encountered,
When biologically active peptide enters digestion by oral way, oral deer collagen peptide is easy
Degraded in digested systematic protein enzyme, so as to lose biological activity.
The content of the invention
It is an object of the invention to provide a kind of skill for protecting deer collagen peptide to degrade from digestive enzyme
Art；, with deer collagen peptide as active component, prepared by deer collagen peptide become liposome, deer collagen for which
Peptidoliposome can resist the degraded of digestive tract protease, make deer collagen peptide active component reach target
Organ plays blood sugar reducing function.Deer collagen peptide is embedded by the present invention by the method for liposome, not shadow
The biological activity of deer collagen peptide is rung, the degraded of digestive tract protease can be resisted again, with good
Application prospect.
For achieving the above object, the technical solution used in the present invention is as follows：
A kind of deer collagen peptide liposome with function of blood sugar reduction, with deer collagen peptide as active component,
Prepared by deer collagen peptide become liposome, with function of blood sugar reduction.
A kind of preparation method with deer collagen peptide liposome described in claim 1,
With in fresh Corium Cervi or Ligamentum cervi or more than two kind as raw material, Corium Cervi or Ligamentum cervi 1～
30%NaOH in 50～100 DEG C boil carry out ungrease treatment within 10～30 minutes after cleaned with pure water
Totally, it is cut to 2～3 square centimeters of fragment, Corium Cervi or Ligamentum cervi fragment are according to 1:1～100 is (former
Material：Buffer) mass ratio adds 0.01～1M buffer, then by 1:10～500 (enzymes：
Raw material) mass ratio adds protease, in 20～50 DEG C of stirring reactions 1～10 hour；Will be upper
The pH for stating reactant liquor is adjusted to 6～8 with 0.1～1M NaOH or 0.1～1M HCI, adds multiple
Synthase is digested, enzyme concentration for reactant quality 0.1～1%, anti-in 20～70 DEG C of stirrings
Answer 1～10 hour, after reaction terminates, 70～100 DEG C inactivate 5～30min；By above-mentioned reaction
2000～8000rpm of liquid is centrifuged 3～10min, collects supernatant, and lyophilization obtains deer collagen
Peptide.
Take and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：0.01～1,
Mixture is dissolved in chloroform and mix homogeneously, and mixture with the mass volume ratio of chloroform is
1:0.1～20 (mg：ml)；Mixed solution forms one layer in 20～80 DEG C of rotary evaporation in vacuo
Adipose membrane.Add deionized water aquation adipose membrane, phospholipid and cholesterol admixture quality and deionized water
The ratio of volume is 1：0.1～10 (mg：Ml), add 0.5～3mm of diameter bead in
30～90 DEG C are stirred 3～60 minutes, are stirred at room temperature 5～60 minutes, form liposome.On
The deer collagen peptide for stating preparation is dissolved in 7.4 phosphate buffers of pH, and preparation becomes concentration and is
The solution of 5～20mg/ml；Take deer collagen peptide and add liposome mixing, deer collagen peptide and liposome
Volume ratio be 1：1～50.Mixture be uniformly dispersed after lyophilization, add deionized water water
Change, deionized water volume is 0.5～20 times of deer collagen peptide liquor capacity, and is incubated at 30～50 DEG C
Educate 5～30 minutes.Mixture is centrifuged 120 minutes under 4 DEG C, 30000g rotating speeds, in collection
It is clearly deer collagen peptide liposome.
Corium Cervi and/or Ligamentum cervi raw material enzymolysis process are two-step method, the first step with single enzyme enzymolysis, the
Two step complex enzyme zymohydrolysis；
Enzyme used by single enzyme enzymolysis is pepsin, trypsin, neutral protease one of which；
Compound enzyme is trypsin, flavor protease, papain, bromelain, alkali
Property protease or neutral protease two of which, three kinds, four kinds or five kinds of enzymes be applied in combination, it is multiple
In synthase, single enzyme is mixed with identical mass ratio.
Deer collagen peptide liposome has the function of blood sugar reduction for suppressing dipeptidyl peptidase IV activity, can
Using in medicine, health food, food additive.
The characteristics of of the invention, is as follows：
1., using the deer collagen peptide liposome prepared by the present invention, deer collagen peptide is neither affected
DPP-IV inhibitory activity, makes deer collagen peptide in digestive tract from digestive tract albumen again
The degraded of enzyme and keep hypoglycemic activity.
2. deer collagen peptide is prepared by the present invention becomes liposome, it is ensured that oral way takes deer
The biological activity of collagen peptide, makes deer collagen peptide use model as food additive etc.
Enclose, expand the range of application of deer collagen peptide, have a good application prospect.
3. the inventive method is simple, it is easy to large-scale production.
Specific embodiment
Embodiment 1
Deer collagen peptide liposome is prepared according to following technique：
With fresh Cervus nippon Temminck Corium Cervi as raw material, Corium Cervi mass concentration 10%NaOH is in 50 DEG C
Boil pure water after carrying out ungrease treatment in 30 minutes to clean up, be cut to 2～3 square centimeters of fragment,
Corium Cervi fragment is according to 1:80 (raw materials：Buffer mass ratio) 0.01M acetate buffer solutions are added,
1 is pressed again:10 (enzymes：Raw material) mass ratio addition pepsin is little in 40 DEG C of stirring reactions 1
When.The pH of above-mentioned reactant liquor is adjusted to into 7 with 1M NaOH, compound enzyme (Trypsin is added
Enzyme, alkaline protease, mass mixing ratio 1:1) digested, enzyme concentration is reactant feed
The 0.1% of quality, in 20 DEG C of stirring reactions 1 hour, reaction terminate after 90 DEG C of inactivation 20min.
Above-mentioned reactant liquor 5000rpm is centrifuged into 5min, supernatant is collected, lyophilization obtains deer collagen
Peptide.
Take 50mg and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：
0.01, mixture is dissolved in 1000ml chloroforms and mix homogeneously, in 40 DEG C of rotary evaporation in vacuo,
Form one layer of adipose membrane.500ml deionized water aquation adipose membranes are added, 0.5～3mm of diameter is added
Bead stir 60 minutes in 90 DEG C, then be stirred at room temperature 60 minutes, form lipid
Body.The deer collagen peptide of above-mentioned preparation is dissolved in the phosphate buffer of pH 7.4, and preparation becomes dense
Spend the solution for 5mg/ml；The deer collagen peptide solution for taking 50ml adds 500ml liposomees
Mixing.Mixture be uniformly dispersed after lyophilization, add 1000ml deionized water aquations, and
It is incubated 15 minutes at 50 DEG C.Mixture is centrifuged 120 minutes under 4 DEG C, 30000g rotating speeds,
Collect supernatant and be deer collagen peptide liposome.
Embodiment 2
With fresh Cervus nippon Temminck Ligamentum cervi as raw material, Ligamentum cervi boils 10 point in 80 DEG C with 5%NaOH
Clock is cleaned up with pure water after carrying out ungrease treatment, is cut to 2～3 square centimeters of fragment, deer
Muscle fragment is according to 1:100 (raw materials：Buffer mass ratio) 0.01M phosphate buffers are added,
1 is pressed again:500 (enzymes：Raw material) mass ratio addition trypsin, in 35 DEG C of stirring reactions 10
Hour.The pH of above-mentioned reactant liquor is adjusted to into 6 with 0.1M HCI, compound enzyme (Fructus Chaenomeliss egg is added
In vain, bromelain, mass ratio 1:1) digested, enzyme concentration for reactant quality 1%,
In 50 DEG C of stirring reactions 10 hours, reaction terminate after 100 DEG C of inactivation 5min.By above-mentioned reaction
Liquid 2000rpm is centrifuged 10min, collects supernatant, and lyophilization obtains deer collagen peptide.
Take 50mg and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：
0.5, mixture is dissolved in 5ml chloroforms and mix homogeneously, in 60 DEG C of rotary evaporation in vacuo, shape
Into one layer of adipose membrane.With 300ml deionized water aquation adipose membranes, the glass of 0.5～3mm of diameter is added
Glass pearl is stirred 20 minutes in 80 DEG C, then is stirred at room temperature 10 minutes, forms liposome.
The deer collagen peptide of above-mentioned preparation is dissolved in 7.4 phosphate buffers of pH, and preparation becomes concentration for 10
The solution of mg/ml；The deer collagen peptide solution for taking 5ml adds the mixing of 100ml liposomees.It is mixed
Compound be uniformly dispersed after lyophilization, add 100ml deionized water aquations, and incubate at 40 DEG C
Educate 20 minutes.Mixture is centrifuged 120 minutes under 4 DEG C, 30000g rotating speeds, collects supernatant
As deer collagen peptide liposome.
Embodiment 3
With fresh Cervus elaphus linnaeuss Ligamentum cervi as raw material, Ligamentum cervi mass concentration 30%NaOH is in 70 DEG C
Boil pure water after carrying out ungrease treatment in 10 minutes to clean up, pure water cleaning after alcohol degreasing process
Totally, it is cut to 2～3 square centimeters of fragment, Ligamentum cervi fragment is according to 1:10 (raw materials：Buffer)
Mass ratio adds 0.01M phosphate buffers, then by 1:50 (enzymes：Raw material) mass ratio addition
Neutral protease, in 45 DEG C of stirring reactions 5 hours.By the pH 0.5M of above-mentioned reactant liquor
NaOH is adjusted to 8, add compound enzyme (Papain, alkaline protease, flavor protease,
Mass ratio is 1:1:1) digested, enzyme concentration is the 0.5% of reactant quality, stirs in 60 DEG C
Reaction 5 hours is mixed, 70 DEG C inactivate 20min after reaction terminates.By above-mentioned reactant liquor 8000rpm
Centrifugation 5min, collects supernatant, and lyophilization obtains deer collagen peptide.
Take 50mg and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：
0.1, mixture is dissolved in 50ml chloroforms and mix homogeneously, in 80 DEG C of rotary evaporation in vacuo,
Form one layer of adipose membrane.With 500ml deionized water aquation adipose membranes, add 0.5～3mm's of diameter
Bead is stirred 60 minutes in 90 DEG C, then is stirred at room temperature 60 minutes, forms liposome.
The deer collagen peptide of above-mentioned preparation is dissolved in 7.4 phosphate buffers of pH, and preparation becomes concentration for 20
The solution of mg/ml；The deer collagen peptide solution for taking 50ml adds the mixing of 500ml liposomees.It is mixed
Compound be uniformly dispersed after lyophilization, add 1000ml deionized water aquations, and incubate at 50 DEG C
Educate 30 minutes.Mixture is centrifuged 120 minutes under 4 DEG C, 30000g rotating speeds, collects supernatant
As deer collagen peptide liposome.
Embodiment 4
With fresh Cervus elaphus linnaeuss Corium Cervi as raw material, Corium Cervi mass concentration 1%NaOH is in 100 DEG C
Boil pure water after carrying out ungrease treatment in 15 minutes to clean up, be cut to 2～3 square centimeters of fragment,
Ligamentum cervi fragment is according to 1:1 (raw material：Buffer) mass ratio addition 0.01M acetate buffer solutions,
1 is pressed again:200 (enzymes：Raw material) mass ratio addition pepsin is little in 25 DEG C of stirring reactions 8
When.The pH of above-mentioned reactant liquor is adjusted to into 7 with 1M NaOH, compound protease (pancreas is added
Protease, alkaline protease, flavor protease, mass ratio are 1:1:1) digested, plus
Enzyme amount for reactant quality 0.8%, in 30 DEG C of stirring reactions 3 hours, reaction terminate after 80 DEG C
Inactivation 25min.Above-mentioned reactant liquor 6000rpm is centrifuged into 6min, supernatant is collected, freezing is dry
It is dry to obtain deer collagen peptide.
Take 50mg and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：
0.5, mixture is dissolved in 5ml chloroforms and mix homogeneously, in 60 DEG C of rotary evaporation in vacuo, shape
Into one layer of adipose membrane.With 300ml deionized water aquation adipose membranes, the glass of 0.5～3mm of diameter is added
Glass pearl is stirred 20 minutes in 80 DEG C, then is stirred at room temperature 10 minutes, forms liposome.
The deer collagen peptide of above-mentioned preparation is dissolved in 7.4 phosphate buffers of pH, and preparation becomes concentration for 10
The solution of mg/ml；The deer collagen peptide solution for taking 5ml adds the mixing of 100ml liposomees.It is mixed
Compound be uniformly dispersed after lyophilization, add 100ml deionized water aquations, and incubate at 40 DEG C
Educate 20 minutes.Mixture is centrifuged 120 minutes under 4 DEG C, 30000g rotating speeds, collects supernatant
As deer collagen peptide liposome.
Claims (6)
1. a kind of deer collagen peptide liposome with function of blood sugar reduction, it is characterised in that：Its with
Deer collagen peptide is active component, and prepared by deer collagen peptide become liposome.
2. the preparation method of deer collagen peptide liposome described in a kind of claim 1, its feature exist
In：
1) with fresh Corium Cervi or Ligamentum cervi or more than two kind as raw material, raw material adopts quality
1～30%NaOH of concentration in 50～100 DEG C boil carry out ungrease treatment within 10～30 minutes after use water
Clean up, be cut to 2～3 square centimeters of fragment, raw material fragment is according to raw material：Buffer
For 1:1～100 mass ratio adds 0.01～1M buffer, then by enzyme：The 1 of raw material:10～
500 was that mass ratio adds protease, in 20～50 DEG C of stirring reactions 1～10 hour；Will be above-mentioned
The pH of reactant liquor 0.1～1M NaOH or 0.1～1M HCI are adjusted to 6～8, add compound
Enzyme is digested, enzyme concentration for reactant feed quality 0.1～1%, is stirred in 20～70 DEG C
Reaction 1～10 hour, after reaction terminates, 70～100 DEG C inactivate 5～30min；Will be above-mentioned anti-
Answer 2000～8000rpm of liquid that 3～10min is centrifuged, collect supernatant, lyophilization obtains Colla cornus cervi
Former peptide；
2) take and be dried phospholipid and cholesterol admixture, phospholipid is 1 with the mass ratio of cholesterol：
0.01～1, mixture is dissolved in chloroform and mix homogeneously, the mass volume ratio of mixture and chloroform
For 1:0.1～20 (mg：ml)；Mixed solution forms one in 20～80 DEG C of rotary evaporation in vacuo
Layer adipose membrane；Add deionized water aquation adipose membrane, phospholipid and cholesterol admixture quality and deionization
The ratio of water volume is 1：0.1～10 (mg：Ml), add the bead of 0.5～3mm of diameter
Stir 3～60 minutes in 30～90 DEG C, be then stirred at room temperature 5～60 minutes, form lipid
Body；The deer collagen peptide of above-mentioned preparation is dissolved in the phosphate buffer of pH6～8, and preparation becomes concentration
For the solution of 5～20mg/ml；Take deer collagen peptide add liposome mixing, deer collagen peptide solution with
The volume ratio of liposome is 1：1～50；Mixture be uniformly dispersed after lyophilization, addition go from
Sub- water hydratable, deionized water volume are 0.5～20 times of deer collagen peptide liquor capacity, and
30～50 DEG C are incubated 5～30 minutes；Mixture is centrifuged under 4 DEG C, 10000～30000g rotating speeds
60～120 minutes, collect supernatant and be deer collagen peptide liposome.
3. according to the preparation method described in claim 2, it is characterised in that：Step 1) in delay
Liquid is rushed for acetate buffer solution or phosphate buffer.
4. according to the preparation method described in claim 2, it is characterised in that：Corium Cervi and/or deer
The enzymolysis process of muscle raw material is two-step method, the first step single enzyme enzymolysis, second step compound enzyme enzyme
Solution；
Enzyme used by single enzyme enzymolysis is pepsin, trypsin, neutral protease one of which；
Compound enzyme is trypsin, flavor protease, papain, bromelain, alkali
Property protease or neutral protease two of which, three kinds, four kinds or five kinds of enzymes be applied in combination, it is multiple
In synthase, each single enzyme is mixed with identical mass ratio.
5. the application of deer collagen peptide liposome described in a kind of claim 1, it is characterised in that：
Deer collagen peptide liposome described in claim 1 has the drop blood for suppressing dipeptidyl peptidase IV activity
Sugared function.
6. according to the application of deer collagen peptide liposome described in claim 5, it is characterised in that：
The deer collagen peptide liposome can make an addition to medicine, health food or food as blood sugar lowering additive
In product additive.
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Cited By (6)
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| CN107712881A (en) * | 2017-11-22 | 2018-02-23 | 吉林海王健康生物科技有限公司 | A kind of preparation method of deerskin glue |
| CN108396052A (en) * | 2018-03-28 | 2018-08-14 | 通化百泉保健食品有限公司 | A kind of industrialized producing technology of deer's sinew albumen oligopeptide |
| CN108484753A (en) * | 2018-03-28 | 2018-09-04 | 通化百泉保健食品有限公司 | A kind of industrialized producing technology of deerskin albumen oligopeptide |
| CN110916038A (en) * | 2019-11-25 | 2020-03-27 | 利和(深圳)生物科技有限公司 | Preparation method of beverage for conditioning blood health |
| CN112569347A (en) * | 2020-12-30 | 2021-03-30 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health foods |
| CN115191617A (en) * | 2022-07-13 | 2022-10-18 | 江南大学 | Preparation method of sodium alginate-stabilized hypoglycemic collagen peptide liposome |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107712881A (en) * | 2017-11-22 | 2018-02-23 | 吉林海王健康生物科技有限公司 | A kind of preparation method of deerskin glue |
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| CN110916038A (en) * | 2019-11-25 | 2020-03-27 | 利和(深圳)生物科技有限公司 | Preparation method of beverage for conditioning blood health |
| CN112569347A (en) * | 2020-12-30 | 2021-03-30 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health foods |
| CN112569347B (en) * | 2020-12-30 | 2023-09-22 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health-care foods |
| CN115191617A (en) * | 2022-07-13 | 2022-10-18 | 江南大学 | Preparation method of sodium alginate-stabilized hypoglycemic collagen peptide liposome |
| CN115191617B (en) * | 2022-07-13 | 2023-10-24 | 江南大学 | Preparation method of sodium alginate stabilized blood glucose-reducing collagen peptide liposome |
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Application publication date: 20170405 |