CN106550762A - A kind of Libo lip post lettuce tongue cuttage breeding method - Google Patents
A kind of Libo lip post lettuce tongue cuttage breeding method Download PDFInfo
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- CN106550762A CN106550762A CN201611115199.8A CN201611115199A CN106550762A CN 106550762 A CN106550762 A CN 106550762A CN 201611115199 A CN201611115199 A CN 201611115199A CN 106550762 A CN106550762 A CN 106550762A
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- cuttage
- libo
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- lettuce tongue
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to a kind of Libo lip post lettuce tongue cuttage breeding method, belongs to Libo lip post lettuce tongue reproduction technique field.The Libo lip post lettuce tongue cuttage breeding method that the application is provided, comprises the following steps：Cuttage raw material is soaked in exogenous hormone aqueous solution, blade of the cuttage raw material for Libo lip post lettuce tongue, the exogenous hormone are naphthalene acetic acid or ABT root-inducing powders；Breeding culture will be carried out in substrate in raw material cuttage to slotting machine after the immersion；60～75%, temperature is 21～28 DEG C to the relative humidity of the breeding culture, and cutting medium temperature is 21～26 DEG C, and intensity of illumination is 60～80% full exposures.The cuttage breeding method rooting rate of present invention offer, planting percent are high, realize the efficient breeding of Libo lip post lettuce tongue.
Description
Technical field
The present invention relates to Libo lip post lettuce tongue reproduction technique field, and in particular to a kind of Libo lip post lettuce tongue cutting propagation side
Method.
Background technology
Libo lip post lettuce tongue pattern is gorgeous, and blade profile leaf color change is enriched, can be used as graceful pot flowers, parterre flower
And Perennial Flowers, with higher ornamental value, some scholars of the country are visited to Libo lip post lettuce tongue reproduction technique at present
Rope and research, also achieve some effects, such as go out Libo lip post lettuce tongue plant using method for tissue culture successful incubation.
The report of Libo lip post lettuce tongue cutting propagation is related in prior art not yet.
The content of the invention
Present invention aim at providing a kind of Libo lip post lettuce tongue cuttage breeding method, improve rooting rate and planting percent be high,
To realize the efficient breeding of Libo lip post lettuce tongue.
The invention provides a kind of Libo lip post lettuce tongue cuttage breeding method, comprises the following steps：
Cuttage raw material is soaked in exogenous hormone aqueous solution, leaf of the cuttage raw material for Libo lip post lettuce tongue
Piece, the exogenous hormone are naphthalene acetic acid or ABT root-inducing powders；
Breeding culture will be carried out in substrate in raw material cuttage to slotting machine after the immersion；
60～75%, temperature is 21～28 DEG C to the relative humidity of the breeding culture, and cutting medium temperature is 21～26
DEG C, intensity of illumination is 60～80% full exposures.
Preferably, the concentration of the exogenous hormone aqueous solution is 0.02～0.5mg/L, time of the immersion is 1～
2.5h。
Preferably, the substrate is combination or the perlite of perlite and yellow soil.
Preferably, the thickness of the substrate is 40～65cm.
Preferably, the water content of the substrate is 50～65%.
Preferably, the cutting process includes that horizontal line cuts and longitudinally cutting.
Preferably, during the cuttage raw material be inserted perpendicularly into or it is flat bury in substrate, it is described to be inserted perpendicularly into or flat bury
Depth is independently selected from 1～2cm.
Preferably, with the KMnO that mass concentration is 0.5～1% before the cuttage4Solution carries out disinfection to bed surface, uses matter
Amount concentration is that 0.3～0.5% carbendazim carries out disinfection to the substrate.
Preferably, after the cuttage 2 weeks, the carbendazim that 1 inferior quality concentration is 0.3～5% is sprayed per 3～7d.
The Libo lip post lettuce tongue cuttage breeding method that the present invention is provided is processed to cuttage raw material using exogenous hormone,
Combination film shed breeds condition of culture, improves Libo lip post lettuce tongue rooting rate and planting percent, realizes Libo lip post lettuce tongue
Efficient cutting propagation.Result of the test shows：The cuttage breeding method rooting rate that the present invention is provided is up to 97%；Planting percent
117%.
Specific embodiment
The invention provides a kind of Libo lip post lettuce tongue cuttage breeding method, comprises the following steps：
Cuttage raw material is soaked in exogenous hormone aqueous solution, leaf of the cuttage raw material for Libo lip post lettuce tongue
Piece, the exogenous hormone are naphthalene acetic acid or ABT root-inducing powders；
Breeding culture will be carried out in substrate in raw material cuttage to slotting machine after the immersion；
60～75%, temperature is 21～28 DEG C to the relative humidity of the breeding culture, and cutting medium temperature is 21～26
DEG C, intensity of illumination is 60～80% full exposures.
Cuttage raw material is soaked in exogenous hormone aqueous solution, in the present invention by the present invention, the cuttage raw material bag
Include the blade or the blade with petiole of Libo lip post lettuce tongue.In the present invention, the cutting process to blade or the blade with petiole
Cut including horizontal line and/or longitudinally cutting.Transverse cuts of the present invention are preferably from 1～2cm of blade base or petiole end
Position proceed by cutting, the transverse cuts and longitudinally cutting number of times are each independently selected from 4～8 times.In the present invention
In, the exogenous hormone is naphthalene acetic acid or ABT root-inducing powders；The present invention does not have special restriction to the source of the exogenous hormone,
Using naphthalene acetic acid well known to those skilled in the art or the commercially available prod of ABT root-inducing powders.
In the present invention, the mass concentration of the exogenous hormone aqueous solution be 0.02～0.5mg/L, more preferably 0.1～
0.2mg/L.The soak time is 1～2.5h, more preferably 2h.In the present invention, the work of the exogenous hormone immersion treatment
With being to change Libo lip post lettuce bryophyte Endogenous Hormones, promote its Organ Differentiation.
After the immersion, the present invention will carry out breeding training in the substrate in the raw material cuttage to slotting machine after the immersion
Support.In the present invention, the substrate is combination or the perlite of perlite and yellow soil, when the substrate is perlite and gold zone
During the combination of soil, the mass ratio of the perlite and yellow soil is preferably (1～5)：1, more preferably 2：1.The present invention is to described
The source of perlite and yellow soil does not have special restriction, using perlite well known to those skilled in the art and the city of yellow soil
Sell product.
In the present invention, the particle diameter of the substrate is preferably 1～4mm, more preferably 2.5mm.In the present invention, it is described to insert
On bed, the thickness of substrate is 40～65cm, more preferably 50cm.The water content of the substrate is 50～65%, more preferably
60%.
In the present invention, during the cuttage raw material be inserted perpendicularly into or it is flat bury in substrate, it is described to be inserted perpendicularly into or flat bury
Depth independently selected from 1～2cm.
In the present invention, preferably with the KMnO that mass concentration is 0.5～1% before the cuttage4Solution disappears to bed surface
Poison, more preferably 0.8%；It is preferred that cutting medium is carried out disinfection with the carbendazim that mass concentration is 0.3～0.5%, more preferably
For 0.4%.
In the present invention, 60～75%, temperature is 21～28 DEG C to the relative humidity of the breeding culture, cutting medium
Temperature is 21～26 DEG C, and illumination condition is 60～80% full exposures.In the present invention, the relative humidity is more preferably 70%,
More preferably 25 DEG C of temperature, more preferably 22 DEG C of the temperature of cutting medium, the intensity of illumination is preferably entered to which using sunshade net
Row is controlled, and the intensity of illumination is more preferably controlled by shading screen is covered on thin film shed, the height of the shed
Preferably 45～60cm, more preferably 50cm.
During the culture, the present invention per 3～7d, more preferably sprays 1 matter preferably after cuttage 2 weeks per 6d
Amount concentration is 0.3～5% carbendazim, and the mass concentration of the carbendazim is more preferably 2.5%.The present invention is to the carbendazim
Source does not have special restriction, using the commercially available prod of carbendazim well known to those skilled in the art.
In the present invention, the water spray process after cuttage is preferably maintained at the water content of substrate according to arid situation decision
50～65%.
Libo lip post lettuce tongue cuttage breeding method of the present invention is done further in detail with reference to specific embodiment
Introduction, technical scheme includes but is not limited to following examples.
Embodiment 1
Cutting medium selects perlite, and it is 0.1mg/L naphthalene acetic acids that exogenous hormone used is mass concentration.
Cutting test is carried out in greenhouse.Slotting machine bottom spreads perlite 45cm, and plastic sheeting shed, shed are built on slotting machine
Intensity of illumination is controlled to 75% full exposure by high 55cm, coverage shading net, controls relative humidity 60%, 25 DEG C of temperature, cuttage base
22 DEG C of matter temperature.
With maternal blade or the leaf production test material with petiole, each test method obtains 50 blades and 50 bands
The blade of petiole is tested, the horizontal and vertical cutting of vanes, using material 1.5h, Ran Houfen after exogenous hormone immersion cutting
In substrate, depth is 1.5cm for other cuttage, checks situation of taking root every 7d after 4d.
First with the KMnO that mass concentration is 0.8% before inserting4Solution carries out disinfection to bed surface, then is 0.3% with mass concentration
Carbendazim cutting medium is carried out disinfection.After cuttage 2 weeks, the carbendazim that 1 inferior quality concentration is 0.3% is sprayed per 7d, according to dry
Damage caused by a drought condition, determines water spray situation, makes the water content of substrate be maintained at 55%.
The rooting rate of the Libo lip post lettuce tongue for finally giving is 90.5%, and planting percent is 103.5%.
Embodiment 2
Cutting medium selects perlite, and it is 0.1mg/L ABT root-inducing powders that exogenous hormone used is mass concentration.
Cutting test is carried out in greenhouse.Slotting machine bottom spreads perlite 55cm, and plastic sheeting shed, shed are built on slotting machine
Intensity of illumination is controlled to 80% full exposure by high 50cm, coverage shading net, controls relative humidity 70%, 27 DEG C of temperature, cuttage base
25 DEG C of matter temperature.
With maternal blade or the leaf production test material with petiole, each test method obtains 50 blades or 50 bands
The blade of petiole is tested, the horizontal and vertical cutting of vanes, using exogenous hormone soak 2h, then respectively cuttage in substrate
On middle about 2cm deep position, then situation of taking root is checked every 7d after 4d.
First with the KMnO that mass concentration is 0.5% before inserting4Solution carries out disinfection to bed surface, then is 0.5% with mass concentration
Carbendazim cutting medium is carried out disinfection.After cuttage 2 weeks, the carbendazim that 1 inferior quality concentration is 2% is sprayed per 5d, according to arid
Situation, determines water spray situation, makes the water content of substrate be maintained at 50%.
The rooting rate of the Libo lip post lettuce tongue for finally giving is 83%, and planting percent is 93.5%.
Embodiment 3
Cutting medium is 1 from mass ratio：1 perlite and yellow soil, exogenous hormone used for mass concentration is
0.2mg/L naphthalene acetic acids.
Cutting test is carried out in greenhouse.Slotting machine bottom spreads substrate 65cm, and plastic sheeting shed is built on slotting machine, and shed is high
Intensity of illumination is controlled to 65% full exposure by 60cm, coverage shading net, controls relative humidity 75%, 23 DEG C of temperature, cutting medium
26 DEG C of temperature.
With maternal blade or the leaf production test material with petiole, each test method obtains 100 blades or 100
Blade with petiole is tested, the horizontal and vertical cutting of vanes, using exogenous hormone soak 2h, then respectively cuttage in base
In matter on about 1.2cm deep position, then situation of taking root is checked every 7d after 4d.
First with the KMnO that mass concentration is 1% before inserting4Solution carries out disinfection to bed surface, then is 0.3% with mass concentration
Carbendazim carries out disinfection to cutting medium.After cuttage 2 weeks, the carbendazim that 1 inferior quality concentration is 4.5% is sprayed per 6d, according to arid
Situation, determines water spray situation, makes the water content of substrate be maintained at 60%.
The rooting rate of the Libo lip post lettuce tongue for finally giving is 90%, and planting percent is 98.5%.
Embodiment 4
Different substrates are affected on the rooting efficiency of Libo lip post lettuce tongue
Other Cutting conditions are set to perlite, yellow soil, perlite+yellow soil with embodiment 1, except for the difference that substrate
(mass ratio is 2：1).
Table 1 is different substrates cuttage root-taking situation, it can be seen that：Perlite, the two kinds of substrate cultures of perlite and yellow soil
Libo lip post lettuce tongue rooting rate and planting percent it is higher, rooting rate up to 90%～95%, planting percent up to 107%～
130%.
1 different substrates cuttage root-taking situation of table
Embodiment 5
Different illumination conditions are affected on the rooting efficiency of Libo lip post lettuce tongue
Other Cutting conditions carry out different settings with embodiment 2, intensity of illumination.Table 2 is to take root under different illumination intensity
Situation, it can be seen that：When intensity of illumination is respectively 60%, 75% and 80%, the rooting rate and planting percent of Libo lip post lettuce tongue are high
There is certain impact to Libo lip post lettuce tongue breeding and make in the rooting rate and planting percent under the conditions of full exposure, different illumination conditions
With under shading condition, take root situation, seedling situation of Libo lip post lettuce tongue is higher compared with rooting rate under the conditions of full exposure and planting percent.
2 different illumination conditions of table are taken root situation
Embodiment 6
The seedling influential effect of hormon species and its concentration to Libo lip post lettuce tongue
Table 3 is the seedling situation after hormon process, and in addition to listed condition in table, other conditions are with embodiment 1.From table
As can be seen that in four kinds of exogenous hormones, the planting percent Jing after naphthalene acetic acid and ABT root-inducing powder immersion treatment is higher than matched group in 3
Indolebutyric acid and heteroauxing, and when the concentration of naphthalene acetic acid and ABT root-inducing powders is in 0.1～0.2mg/L, planting percent highest.
Table 4 is that naphthalene acetic acid or ABT root-inducing powders process situation of taking root, as can be seen from Table 4, Jing after naphthalene acetic acid process, Libo lip post lettuce
The rooting rate of tongue is up to 97%, and planting percent is up to 112%；Jing after the process of ABT root-inducing powders, the rooting rate of Libo lip post lettuce tongue is up to
86%, planting percent is up to 97%.
3 hormon of table processes situation of taking root
4 naphthalene acetic acid of table or ABT root-inducing powders process situation of taking root
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of Libo lip post lettuce tongue cuttage breeding method, comprises the following steps：
Cuttage raw material is soaked in exogenous hormone aqueous solution, blade of the cuttage raw material for Libo lip post lettuce tongue, institute
Exogenous hormone is stated for naphthalene acetic acid or ABT root-inducing powders；
Breeding culture will be carried out in substrate in raw material cuttage to slotting machine after the immersion；
60～75%, temperature is 21～28 DEG C to the relative humidity of the breeding culture, and cutting medium temperature is 21～26 DEG C, light
It is 60～80% full exposures according to intensity.
2. method according to claim 1, it is characterised in that the concentration of the exogenous hormone aqueous solution is 0.02～
0.5mg/L, the time of the immersion is 1～2.5h.
3. method according to claim 1, it is characterised in that the substrate is combination or the Margarita of perlite and yellow soil
Rock.
4. the method according to claim 1 or 3, it is characterised in that the thickness of the substrate is 40～65cm.
5. the method according to claim 1 or 3, it is characterised in that the water content of the substrate is 50～65%.
6. method according to claim 1, it is characterised in that the cutting process includes horizontal line cutting and longitudinally cutting.
7. method according to claim 1, it is characterised in that raw material is inserted perpendicularly into or flat buries substrate during the cuttage
In, it is described to be inserted perpendicularly into or equal the depth buried independently selected from 1～2cm.
8. method according to claim 1, it is characterised in that with the KMnO that mass concentration is 0.5～1% before the cuttage4
Solution carries out disinfection to bed surface, the substrate is carried out disinfection with the carbendazim that mass concentration is 0.3～0.5%.
9. method according to claim 1, it is characterised in that after the cuttage 2 weeks, 1 inferior quality concentration is sprayed per 3～7d is
0.3～5% carbendazim.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107318623A (en) * | 2017-08-04 | 2017-11-07 | 贵州省林业科学研究院 | A kind of method of Begonia masoniana cutting propagation |
| CN109005966A (en) * | 2018-07-10 | 2018-12-18 | 深圳市仙湖植物园管理处（深圳市园林研究中心） | A kind of cuttage breeding method of Gesneriaceae |
| CN112931073A (en) * | 2021-01-27 | 2021-06-11 | 中国科学院华南植物园 | Method for naturally regressing Yaoshan moss |
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| CN107318623A (en) * | 2017-08-04 | 2017-11-07 | 贵州省林业科学研究院 | A kind of method of Begonia masoniana cutting propagation |
| CN109005966A (en) * | 2018-07-10 | 2018-12-18 | 深圳市仙湖植物园管理处（深圳市园林研究中心） | A kind of cuttage breeding method of Gesneriaceae |
| CN112931073A (en) * | 2021-01-27 | 2021-06-11 | 中国科学院华南植物园 | Method for naturally regressing Yaoshan moss |
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Application publication date: 20170405 |