CN104521539A - Gold leaf acer negundo twig cutting seedling method - Google Patents
Gold leaf acer negundo twig cutting seedling method Download PDFInfo
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- CN104521539A CN104521539A CN201510026553.9A CN201510026553A CN104521539A CN 104521539 A CN104521539 A CN 104521539A CN 201510026553 A CN201510026553 A CN 201510026553A CN 104521539 A CN104521539 A CN 104521539A
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- 244000046151 Acer negundo Species 0.000 title claims abstract description 36
- 235000004422 Acer negundo Nutrition 0.000 title claims abstract description 34
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- 238000000034 method Methods 0.000 title claims abstract description 24
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- 235000019362 perlite Nutrition 0.000 claims abstract description 10
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 8
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- 230000006698 induction Effects 0.000 claims abstract description 4
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- 239000007921 spray Substances 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 230000008774 maternal effect Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
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- 239000004745 nonwoven fabric Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
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- 210000004027 cell Anatomy 0.000 description 5
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
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- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 238000004088 simulation Methods 0.000 description 3
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- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 2
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- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
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- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
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- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a gold leaf acer negundo twig cutting seedling method which comprises the steps of cutting bed preparation, cutting stem selection, cutting shoot making, cutting shoot hormone rooting induction, cutting, post-cutting management and the like. Perlite is adopted as a substrate of a cutting bed, and a magnetic field network, an electric field network and an automatic atomizing system are arranged; cutting stems are selected within May, June and July; cutting stems with the diameter ranging from 4 mm to 6 mm are selected as cutting shoots and soaked in a growth regulating agent for 1 min to 2 h; a straight cutting method is adopted for cutting the cutting shoots into the seedbed, the automatic atomizing system carries out automatic atomization after cutting is finished when the air humidity is smaller than 90%; in the period from cutting to callus forming, the shading rate is kept 75%, the relative humidity is kept from 90% to 91%, and the temperature is kept from 30 DEG C to 40 DEG C; in the middle and later periods of cutting, the air humidity is controlled to range from 70% to 80%, the temperature is controlled to range from 30 DEG C to 40 DEG C, and the cutting shoots are processed by 100000 V voltage for 2 h every day. According to the gold leaf acer negundo twig cutting seedling method, the cutting environment most suitable for growth of gold leaf acer negundo twigs is obtained through screening by adjusting and controlling cutting shoot obtaining time, the cutting environment, the cutting shoot selection, the cutting substrate selection, the selection of hormone promoting rooting and other aspects, and the method is stable and efficient for gold leaf acer negundo twig cutting propagation.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, be specifically related to gold leaf Acer negundo. L epicormic branch cuttage technology.
Background technology
Gold leaf Acer negundo. L (Acer negundo ' Kellys gold ') to be fallen leaves high megaphanerophyte for Aceraceae Acer L, the cultivar You Cheng Ash leaf maple of North America Acer negundo. L (Acer negundo), ash-leaved maple, originate in North America, in North America, Europe is widely used in urban afforestation.Russia has plants and in a large number for building shelter forest, and the end of the eighties in last century just introduces China, tree crown ball-shaped, sprig is smooth, and often by white wax powder, imparipinnate leaf is to life, the very large 5-7 piece of leaflet, leaf look soft, and blade shows as yellow in the whole season of growth, spring bright yellow, fade to yellow green, summer not burnt leaf, raceme, flower without lobe; Infructescence is sagging, and two wings are carried out and acutangulated, and May, first leaf was bloomed, and September, fruit was ripe.
Happiness light, happiness Cold and cool climate, resistance to-40 DEG C ~-45 DEG C low temperature, drought-enduring, waterlogging, less demanding to soil property, no matter fertilizer is lean or soda acid soil all can grow, adaptable, cold tolerance is strong, and have wider Bioclimatic analysis, damage by disease and insect is few, is the beautiful ornamental tree species of urban beautification, green barren hill, color.Spring and summer autumn green dry, safflower is graceful and bold and unrestrained; Winter is green dryly becomes red dry, warm and ardent, is described as " the princess tree " in street tree.Shade tree, street tree, specimen tree should be made, flourishing, be easy to form large-area colony view, having the very large market demand, is the important middle level trees showing autumn scenery in amenity forest, in numerous autumn scenery Colored-leaf Plants, gold leaf Acer negundo. L is taken the course of its own, and has glamour.Gold leaf Acer negundo. L not only leaf coloured gold Huang is bright, and amount of growth is large, is high megaphanerophyte rare in Colored-leaf Plants family.
Gold leaf Acer negundo. L planting seed variability is large, be unfavorable for the merit ensureing this kind, current gold leaf Acer negundo. L breeding adopts propagation by grafiting more, but propagation by grafiting is higher to technical requirement, cultivate masterful technique work, and limit by stock seeds, the cultivation of stock also will expend certain human and material resources, overall reproductive speed is slow, and cost is higher.
Epicormic branch cuttage is used widely as a kind of technology of applicable scale high efficiency quick breeding; there is advantages such as taking root rapidly easily, well developed root system, survival rate are high, fringe bar abundance, cycle of emerging are short; and having superiority of maternal plant can be retained completely; continuity color tree species leaf look proterties can be kept well, be well suited for the breeding for color tree species in gardens.Carry out gold leaf Acer negundo. L epicormic branch cuttage, and cuttings collection is pruned with sparse branching in summer combine, the branch removed can be turned waste into wealth, utilize once again, the breeding and Seedling Industrialization Development promoting gold leaf Acer negundo. L is had great importance.
Research conclusion about gold leaf Acer negundo. L epicormic branch cuttage is inconsistent, and the index weighing growth is too single, and relevant result of study also shows that all difficult seeds that take root of Aceraceae plant, containing internal hormones and related enzyme such as tannin, belong to and induce root base rooting type.
Summary of the invention
The problem that the present invention need solve is not enough for prior art, advanced high-pressure electrostatic magnetization seedling growing process is utilized to promote cuttage rooting, simultaneously from cuttage environment, hormone kind, hormone concentration, cutting time, adopt the aspects such as fringe position system regulation carried out to gold leaf Acer negundo. L cottage propagation, to the optimum condition of screening gold leaf Acer negundo. L softwood cutting propagation, for gold leaf Acer negundo. L softwood cutting propagation provides a kind of method of stability and high efficiency.
Object of the present invention realizes by following technical scheme:
A kind of gold leaf Acer negundo. L epicormic branch cutting method, its feature is, comprises the steps:
(1) slotting machine prepares:
Slotting machine is located in temperature control green house, and employing perlite is matrix, the thick perlite of paving 8-10cm, lay magnet under it, between magnet and perlite, make separation layer with non-woven fabrics, above slotting machine, upper electric field net is laid at 40cm place, magnetic underlayer lays lower electric field net, and upper electric field side off the net lays automatic spraying system;
(2) cutting is chosen
Select the cloudy day, take robust growth on gold leaf Acer negundo. L maternal plant, give birth to spray then as cutting without damage by disease and insect, cutting access time is 5-7 month;
(3) cuttings makes:
Select diameter 4mm-6mm cutting as cuttings, each cuttings is cut into 10cm-15cm, retains 2-3 joint, and 2cm place on bud, upper end is cut into flat mouth, and lower end is truncated in the lower 0.5cm place of joint, and otch is level and smooth; Each cuttings retains 1/2nd blades of less a slice in the compound leaf of side; By described cuttings soaking disinfection 15 minutes;
(4) the hormone root induction of cuttings and cuttage:
In 50-800ppm growth regulator, 1min-2h is soaked by bottom the cuttings after disinfecting; Adopt the cuttage of straight cutting method in the slotting machine described in step (1), inserting the native degree of depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, line-spacing 7cm;
(5) manage after cuttage:
In temperature control green house, during cuttage to Callus formation, keep shading rate 75%, relative moisture 90-91%, 30 DEG C-40 DEG C; By simulation blade sensor, as air humidity < 90%, start auto spraying, when reaching 90-91% to relative moisture, stop spraying; Then reduce air humidity gradually to the phase of taking root after cuttage middle and later periods and Callus formation, control air humidity at 70%-80%, temperature 30 DEG C-40 DEG C; Be equipped with high-voltage generator simultaneously, by upper and lower electric field net every day with 100,000 volts of voltages to cuttings process 2 hours.
Described cutting time is late July.
The growth regulator of described step (4) is IBA, and concentration is 50ppm, and soak time is 2h.
The present invention relative to the beneficial effect of prior art is:
Cuttage and seedling culture method of the present invention, accelerates gold leaf Acer negundo. L spray and takes root, and promotes that cuttings is grown, not only shorten growing-seedling period, combine the useless branch cut when utilizing training in summer or grafting simultaneously and make cuttings, reduce cost, be conducive to the industrialization of gold leaf Acer negundo. L cottage propagation and carry out.
Cottage method of the present invention, take root in a large number in two weeks, rooting rate reaches 81.11%, the cuttings survival rate 93.4% after taking root.
It is poor that electrostatic field of the present invention produces micro-current level under elevated pressure conditions, and cellular endogenous growth hormone is transported from electronegative potential to high potential, and negative ions moves, break cuttings in vitro after of short duration dormancy.Potential difference is the important motivity of Polar Transport of Auxin, on the one hand because film potential strengthens, make hormone transmission faster, after living cells damaged, its current of injury produced is strengthened therefrom, facilitate the transmission of information and material, injury is produced and repairs responsing reaction, thus promote cuttings Callus formation.Cell membrane carries out the important channel of mass exchange as cell and surrounding physiological environment on the other hand, has extremely crucial effect.When in certain electrostatic field to plant, cell membrane can be made excited, improve the permeability of ion, and then promote the process of Cell uptake ion, impel cuttings cells in vivo to break up, improve the speed of the mass exchange inside and outside cell membrane, be rapidly converted into cell of taking root, the activity of activated in advance enzyme, accelerates metabolism, improves the photosynthetic rate of cuttings blade and respiratory rate thus promotes the conveying activity of moisture and nutrient in cuttings body.
Embodiment
Below by way of specific embodiment, explanation is further explained to technical solution of the present invention.
(1) seedbed prepares:
Experiment is carried out in the intelligent temperature control booth of Hefei forestry Advanced Technology Center, booth area about 2000 ㎡, totally 98 seedbeds.Employing perlite is matrix, and seedbed specification is 10.0m × 1.2m × 0.4m, and for ease of operation and slotting machine draining, center, seedbed is higher than both sides 2cm, and the bottom will reserve osculum.Tile in seedbed the thick perlite of 10cm, magnet is laid under it, separation layer is made with non-woven fabrics between magnet and perlite, above slotting machine, upper electric field net is laid at 40cm place, and magnetic underlayer lays lower electric field net, and upper electric field side off the net lays automatic spraying system, be equipped with simulation blade sensor, according to slotting machine moisture situation water spray, be equipped with high-voltage generator simultaneously, every day by 100,000 volts of low pressure to cuttings process 2 hours.
(2) cutting is chosen:
Select cloudy day take robust growth on maternal plant, without damage by disease and insect then give birth to spray, choose the part that in branch, hypomere degree of lignification is high and do cutting.And respectively May 24, June 24, respectively adopted a cutting July 24 and carry out cutting time Experimental Comparison.
(3) cuttings makes:
Conventionally cuttings is cut into 10cm-15cm (retaining 2 ~ 3 joints), diameter is the cuttage branch of 4mm-6mm, and 2cm place on bud, upper end is cut into flat mouth, and lower end is truncated in the lower 0.5cm place of joint, and otch is level and smooth.Thickness according to cuttings selects 1/2nd blades retaining less a slice in the compound leaf of side.Cuttings 30 is a bundle, puts into 25% carbendazol wettable powder soaking disinfection 15 minutes.And select cutting top, middle part, bottom to carry out comparative trial as cuttings respectively, matrix, hormone, cutting time all adopt cuttings in the middle part of branch to the test that gold leaf Acer negundo. L cottage propagation affects.
(4) the hormone root induction of cuttings and cuttage:
External source root-promoting hormone is ABT1 root-inducing powder, methyl α-naphthyl acetate (NAA), indolebutyric acid (IBA), heteroauxin (IAA), NAA+IBA (isoconcentration mixing), ABT1 needs first to add auxiliary solvent when dissolving, then uses warm water solution.Methyl α-naphthyl acetate (NAA), indolebutyric acid (IBA) and heteroauxin (IAA) need to add a little sodium hydroxide hydrotropy when dissolving.In 50ppm, 100ppm, 200ppm growth regulator, 2h is soaked by bottom the cuttings after disinfecting; Dip 1min 500, in 800ppm growth regulator, solution did not have base portion 3cm, and every 30 cuttings are one group, experiment in triplicate and with clear water process in contrast, carry out the comparative trial of root-promoting hormone and concentration.Cutting position, cutting time, cuttage environment all adopt IBA50ppm to the test that gold leaf Acer negundo. L cottage propagation affects.
Adopt straight cutting method, matrix watered permeable before cuttage, cuttage immediately under moisture state after HORMONE TREATMENT, presses cuttings surrounding matrix gently after cuttage, make it fully to contact with cuttings, and inserting the native degree of depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, line-spacing 7cm.Cuttage terminates rear spray 25% carbendazol wettable powder 400 times of liquid, prevents fungal infection cuttings.
(5) manage after cuttage:
In temperature control green house, during cuttage to Callus formation, keep shading rate 75%, relative moisture 90-91%, 30 DEG C-40 DEG C; By simulation blade sensor, as air humidity < 90%, start auto spraying, when reaching 90-91% to relative moisture, stop spraying; Then reduce air humidity gradually to the phase of taking root after cuttage middle and later periods and Callus formation, control air humidity at 70%-80%, temperature 30 DEG C-40 DEG C; Be equipped with high-voltage generator simultaneously, by upper and lower electric field net every day with 100,000 volts of voltages to cuttings process 2 hours.Cultivate and can become transplantation of seedlings in one month.
The present embodiment relevant experimental data is in Table 1-3.
The impact that the process of table 1 hormon is taken root on gold leaf Acer negundo. L epicormic branch cuttage
The different cutting time of table 2 is to gold leaf Acer negundo. L epicormic branch cuttage Rooting effect
The different cutting position of table 3 is to gold leaf Acer negundo. L epicormic branch cuttage Rooting effect
Claims (3)
1. a gold leaf Acer negundo. L epicormic branch cutting method, is characterized in that, comprise the steps:
(1) slotting machine prepares:
Slotting machine is located in temperature control green house, and employing perlite is matrix, the thick perlite of paving 8-10cm, lay magnet under it, between magnet and perlite, make separation layer with non-woven fabrics, above slotting machine, upper electric field net is laid at 40cm place, magnetic underlayer lays lower electric field net, and upper electric field side off the net lays automatic spraying system;
(2) cutting is chosen
Select the cloudy day, take robust growth on gold leaf Acer negundo. L maternal plant, give birth to spray then as cutting without damage by disease and insect, cutting access time is 5-7 month;
(3) cuttings makes:
Select diameter 4mm-6mm cutting as cuttings, each cuttings is cut into 10cm-15cm, retains 2-3 joint, and 2cm place on bud, upper end is cut into flat mouth, and lower end is truncated in the lower 0.5cm place of joint, and otch is level and smooth; Each cuttings retains 1/2nd blades of less a slice in the compound leaf of side; By described cuttings soaking disinfection 15 minutes;
(4) the hormone root induction of cuttings and cuttage:
In 50-800ppm growth regulator, 1min-2h is soaked by bottom the cuttings after disinfecting; Adopt the cuttage of straight cutting method in the slotting machine described in step (1), inserting the native degree of depth is the 1/3-1/4 of cutting length, spacing in the rows 5cm, line-spacing 7cm;
(5) manage after cuttage:
In temperature control green house, during cuttage to Callus formation, keep shading rate 75%, relative moisture 90-91%, 30 DEG C-40 DEG C; As air humidity < 90%, start auto spraying, when reaching 90-91% to relative moisture, stop spraying; Then reduce air humidity gradually to the phase of taking root after cuttage middle and later periods and Callus formation, control air humidity at 70%-80%, temperature 30 DEG C-40 DEG C; Be equipped with high-voltage generator simultaneously, by upper and lower electric field net every day with 100,000 volts of voltages to cuttings process 2 hours.
2. a kind of gold leaf Acer negundo. L epicormic branch cutting method according to claim 1, it is characterized in that, described cutting time is late July.
3. a kind of gold leaf Acer negundo. L epicormic branch cutting method according to claim 1, it is characterized in that, the growth regulator of described step (4) is IBA, and concentration is 50ppm, and soak time is 2h.
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| CN108464178A (en) * | 2018-03-13 | 2018-08-31 | 贵州省山地资源研究所 | A kind of epicormic branch cutting method of kadsurae coccineae |
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| CN107494284B (en) * | 2017-10-16 | 2019-01-29 | 广东德康农业科技有限公司 | A kind of breeding of the fleece-flower root and cultural method |
| CN108432527A (en) * | 2018-01-11 | 2018-08-24 | 天津绿茵景观生态建设股份有限公司 | A kind of Acer negundo. L Technique on Cuttage Propagation |
| CN108464178A (en) * | 2018-03-13 | 2018-08-31 | 贵州省山地资源研究所 | A kind of epicormic branch cutting method of kadsurae coccineae |
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