CN106520615A - Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof - Google Patents
Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof Download PDFInfo
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Abstract
The invention discloses a composite microbial system for quickly degrading maize straw, a preparation method thereof and a pretreatment method for conducting quick maize straw degrading through the composite microbial system. The component of composite microbial system for quickly degrading the maize straw is an optimal combination of fungus, bacteria and actinomycetes, the composition is more diversified, and accordingly the composite microbial system has a better degrading effect on the maize straw. Through the pretreatment method adopting the composite microbial system to pretreat the maize straw, the microstructure of the maize straw is damaged, the hydrolysis process is accelerated, and the accessibility of lignocelluloses is remarkably improved, so that the straw degrading efficiency and stability are improved notably, and resource utilization of the maize straw is further facilitated.
Description
Technical field
The present invention relates to microbe to screen method and technology field, and in particular to a kind of compound bacteria of fast degradation corn straw
System and preparation method thereof and the preprocess method of fast degradation straw is carried out using the composite microbial system.
Background technology
China is large agricultural country, all kinds of agricultural straw resource very abundants, and agricultural crop straw mainly by cellulose, half
Cellulose and lignin three parts composition, are difficult to be decomposed by the microorganisms under naturalness, so in soil after straw directly returning to field
The cycle for being decomposed by the microorganisms conversion in earth is long, it is difficult to used as the source of manure of this season crop.In the past, in rural energy shortage, yule logs
In the case of shortage, most of straw is used as fuel combustion by peasant and falls.But the development and Rural Energy with rural economy
The solution of source problem, straw are not re-used as fuel, and crops are become increasingly conspicuous by the phenomenon of centralized burning in harvesting season straw, no
Only pollute environment, endanger the healthy and full of smoke of people, affect land communications and flight safety.
At present, the main Land use systems of straw have straw-returning, fodder utilization, gasifying stalk, solidification and carbonization, give birth to
Produce culture base-material, commercial Application of edible fungi etc..
Straw-returning include on the spot also field, quickly make compost with compost etc..Wherein, can also to increase soil organic in field on the spot for straw
Matter content, fertilizing soil, the sustainable development to agricultural are significant, but also problems are not yet solved.First, straw
Carbon-nitrogen ratio（C/N）Height, not only bad for soil microbial degradation, and also can result in crop nitrogen stress and undergrowth；Its
Secondary, straw is also possible to part of propagation disease, causes crop failure etc..And quickly make compost and then there is cellulose with compost and be difficult to degrade
Problem.
Fodder mainly includes ensiling ammonification, fermentation method and production single cell protein using technology.Can be used as in China
The agricultural crop straw of feedstuff has various, but with common nutritional characteristic：Protein, soluble-carbohydrate, mineral and
Carotene carotene content is low, and crude fiber content is high, therefore digestibility is low, and palatability is poor.Palatability to be improved, improves digestibility, deposits
In the degradation problem of cellulose.Commercial Application includes papermaking, biodegradation production starch, ethanol, acetic acid etc., easily causes serious
Environmental pollution, and equally exist the degradation problem of cellulose.
At this stage, the research to its biomass energy conversion is a lot, and wherein stalk anaerobic fermentation producing methane is current grinding
Study carefully one of focus.In general, in corn straw composition, the content of lignin be the content of 17.5%, cellulose be 37.3%,
It is that 2.0%, other content of material are 18.5% for 6.1%, acylate content that the content of hemicellulose is 20.6%, content of ashes.
Wherein cellulose and hemicellulose are the sources of fermentable sugars, but as the presence of lignin causes corn straw hydrolytic process to delay
Slowly, hydrolysis degree is low, and then have impact on follow-up acidifying and gas generation process.
Therefore, studying suitable preprocess method carries out pretreatment to corn straw, so as to destroy the microstructure of straw,
Accelerate hydrolysis process, have in terms of the recyclings such as the gas generation property with corn straw as waste biogas important to improving
Meaning, become this area technical problem urgently to be resolved hurrily.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, there is provided one kind can quickly, efficient degradation
The composite microbial system and its biodegrading process of corn straw, is significantly improved the efficiency and stability of degrading maize straws.
For reaching aforementioned invention purpose, the technical solution adopted in the present invention includes：
A kind of composite microbial system of fast degradation corn straw, including funguses, antibacterial and actinomycetes, wherein funguses include close viscous pleat
Bacterium, Phanerochaete chrysosporium, Coriolus Versicolor, Trichoderma viride and aspergillus niger, antibacterial include Bacillus circulans, P. aeruginosa
Bacterium, actinomycetes include Streptomyces badius.
A kind of preparation method of the composite microbial system, comprises the steps：
Prepare fluid medium：Wherein, fluid medium includes nutrient culture medium, LB culture medium, PDA culture medium and ferment
Female powder, starch culture-medium；
Single culture amplification culture：Each fluid medium to preparing carries out high temperature sterilize, after being cooled to room temperature, uses inoculating loop
Taking a small amount of each strain carries out spawn culture；
Generate composite microbial system：Each strain of slant preservation is activated by solid state rheology respectively, the difference after activation single
One strain is inoculated into different liquids culture medium respectively, and places it in culture in isothermal vibration incubator；Then by different strain
Bacterium solution mix homogeneously.
Preferably, prepare nutrient culture medium：Take 5 grams of peptone, 30 grams of Carnis Bovis seu Bubali cream, 5 grams of Sodium Chloride, distilled water 1000
Milliliter, sterilizes 30 minutes at 121 DEG C, for the culture of Bacillus circulans；Prepare LB culture medium：Take 5 grams of yeast extract, egg
White 10 grams of peptone, 10 grams of Sodium Chloride, 1000 milliliters of distilled water sterilize 30 minutes at 121 DEG C, for the culture of Pseudomonas aeruginosa；
Prepare yeast powder, starch culture-medium：2 grams of yeast extract is taken, 10 grams of soluble starch, 1000 milliliters of distilled water go out at 121 DEG C
Bacterium 30 minutes, for the culture of Streptomyces badius；Prepare PDA culture medium：Take 1000 milliliters of Rhizoma Solani tuber osi extracting solution, glucose 20
Gram, 115 DEG C sterilize 30 minutes, for Phanerochaete chrysosporium, Coriolus Versicolor, Trichoderma viride, aspergillus niger, gloeophyllum trabeum training
Support.
Preferably, single culture amplification culture refers to the culture medium that will be prepared, and sterilizes 30 minutes at high temperature, is cooled to
After room temperature, 1～2 ring of strain is taken in triangular flask with inoculating loop, and placed it in isothermal vibration incubator, 30 DEG C of constant temperatures
Lower 120r/min is continuously cultivated 2 days.
Preferably, generation composite microbial system is referred to and is activated each strain of slant preservation respectively by solid state rheology,
Different single cultures after activation are inoculated into different liquids culture medium respectively, and place it in isothermal vibration incubator, 30 DEG C
Under constant temperature, 120r/min is continuously cultivated 2 days, then by the bacterium solution mix homogeneously of different strain.
A kind of preprocess method using the composite microbial system fast degradation corn straw, takes 30 grams of straw, is placed in 250 millis
Rise in triangular flask, according to solid-liquid mass ratio 1：5 add bacterium solution, are sealed with sealed membrane, to maintain base in triangular flask after mix homogeneously
The moisture content of matter；Cultivate 14 days under 30 DEG C of constant temperatures, humid control is more than 80%.
Compared with prior art, it is an advantage of the invention that：
（1）The present invention provide degrading maize straws microbial composite bacteria system be funguses, antibacterial and actinomycetic optimum organization,
Destructive effects can be produced to surface integument by secreting digestive enzyme, the straw rough surface, bulk Jing after compound bacteria pretreatment,
There is a large amount of projections and hole in the surface of coarse structure, lignocellulosic structure changes, and increased the exposure of cellulose
Product, is conducive to microorganism mycelia be directly entered inside straw, internal lignocellulose is degraded by secreting digestive enzyme.
As a result show, during compound bacteria pretreated straw, significantly improve the accessibility of lignocellulose, further significantly improve wood
The utilization ratio of matter cellulose.
（2）The present invention creatively prepares composite microbial system, and research shows, by the composite microbial system of the present invention to corn stalk
Stalk carries out pretreatment, so as to destroy the microstructure of straw, accelerates hydrolysis process so as to degradation efficiency than single culture or other
Flora is significantly improved.
（3）Composite microbial system is prepared by optimizing special parameter, fast and efficiently pretreatment is realized, is further significantly carried
It is high with corn straw as the gas generation property of waste biogas.
Description of the drawings
Fig. 1 is microbial growth curve in corn straw preprocessing process.
Fig. 2 is the change of pH value in corn straw preprocessing process.
Fig. 3 is the change of COD value in corn straw preprocessing process.
Fig. 4 is corn straw scanning electron microscope image before pretreatment.
Fig. 5 is corn straw scanning electron microscope image after natural packing pretreatment.
Fig. 6 is corn straw scanning electron microscope image after compound bacteria pretreatment.
Specific embodiment
It is that above and other objects, features and advantages of the invention can be become apparent, hereafter with the preferable of the present invention
As a example by embodiment, it is described below in detail.
The present invention, is creatively prepared using the characteristic of different strain according to corn straw main component as shown in table 1
Go out a kind of composite microbial system of fast degradation corn straw, the composite microbial system includes funguses, antibacterial and actinomycetes, and wherein funguses include
Gloeophyllum trabeum, Phanerochaete chrysosporium, Coriolus Versicolor, Trichoderma viride and aspergillus niger, antibacterial include that Bacillus circulans, Aerugo are false
Zymomonas mobiliss, actinomycetes include Streptomyces badius.
The main component of 1 corn straw of table
| Raw material | C/% | N/% | C/N | Cellulose/% | Hemicellulose/% | Lignin/% | Total solid TS/% | Volatile solid VS/% |
| Straw | 49.41±1.87 | 1.48±0.14 | 33.39 | 36.53±1.27 | 27.93±1.62 | 15.38±0.92 | 90.92±0.73 | 87.49±0.31 |
Corn straw mainly by cellulose, hemicellulose and lignin these into being grouped into.For the degraded of these materials is needed
Want the synergism of multiple-microorganism.Whiterot fungi in the present embodiment（Phanerochaete chrysosporium and Coriolus Versicolor in compound bacteria）
There is to lignin good degradation capability, the lignin degradation being wrapped in outside cellulose can be made, be conducive to cellulose exposed, from
And strengthen degradation efficiency of other microorganisms to cellulose.Meanwhile, the aspergillus niger and Trichoderma viride in the present embodiment is to corn stalk
Cellulose and hemicellulose in stalk has preferable degradation capability.Actinomycetes and antibacterial in this enforcement can be from structure in addition
Change the structure of lignocellulose, improve the accessibility of cellulose.
Invention preferably provides a kind of method of composite microbial system fast degradation corn straw, comprises the following steps：
The compound method of fluid medium is as follows：Nutrient culture medium：Peptone 5g, Carnis Bovis seu Bubali cream 30g, Sodium Chloride（NaCl）
5g, distilled water 1000ml, 121 DEG C of sterilizing 30min are used for the culture of Bacillus circulans；LB culture medium：Yeast extract 5g, egg
White peptone 10g, Sodium Chloride（NaCl）10g, distilled water 1000ml, 121 DEG C of 30 min of sterilizing, for the culture of Pseudomonas aeruginosa；
Yeast powder, starch culture-medium：Yeast extract 2g, soluble starch 10g, distilled water 1000ml, 121 DEG C of sterilizing 30min, are used for
The culture of Streptomyces badius；PDA culture medium：Rhizoma Solani tuber osi extracting solution 1000ml, glucose 20g, 115 DEG C of sterilizing 30min, for Huang
The flat lead fungi of archespore hair, Coriolus Versicolor, Trichoderma viride, aspergillus niger, the culture of gloeophyllum trabeum.
Single culture amplification culture：By each fluid medium for preparing, sterilize 30min at high temperature, is cooled to room temperature
After be placed in triangular flask, take 1～2 ring of each strain in triangular flask with inoculating loop, and place it in isothermal vibration incubator, 30
DEG C, 120r/min continuously cultivate 2d, will be enlarged by cultivate after each strain carry out slant preservation.
Compound bacteria pretreatment：The each strain obtained Jing after amplification culture of slant preservation is carried out by solid state rheology respectively
Activation, activation after different single cultures be inoculated into different liquids culture medium respectively, and place it in isothermal vibration incubator
Interior, under 30 DEG C of constant temperatures, 120r/min continuously cultivates 2d.Then by the bacterium solution mix homogeneously of different strain, take standby straw
30g, is placed in 250mL triangular flasks, according to solid-liquid mass ratio 1:5 add bacterium solution, are sealed with sealed membrane after mix homogeneously, to maintain
The moisture content of triangular flask mesostroma.30 DEG C of constant temperature culture 14d, humid control is more than 80%.
Wherein, the high temperature described in single culture amplification culture is more than 121 DEG C.
Impact of the above-mentioned preprocess method to the degradation efficiency of corn straw is utilized using the technique study of controlled trial.Examination
During testing with the pretreatment of corn straw natural packing as a control group.The material during 2d is measured by sampling different pretreatments
In growth of microorganism amount, pH value and COD value, the content to lignin, cellulose, hemicellulose in straw before and after pretreatment
It is measured, and uses scanning electron microscopic observation microstructure change, relative analyses material characteristic reason of changes.
Wherein, lignocellulose assay method：Using normal form WATER-WASHING METHOD to the cellulose in straw, hemicellulose and wooden
Cellulose content is measured.The measure of growth of microorganism amount：Using HP8453 types（Shanghai Anjelen Sci. ＆ Tech. Inc produces）Point
The OD values of light photometer determination sample at the 600nm, used as microbial cell concentration.The measure of pH value：Using PHS-3C types（South
Jing Ke ring analyses Instrument Ltd.）PH meter is measured.The measure of COD value：The supernatant after fermentation liquid stands is taken, is adopted
KHCN-200A type COD ammonia nitrogen determination instrument（Ring analyses instrument company limited of Nanjing section produces）It is measured.
Scanning electron microscope analysis：The straw sample before and after a small amount of pretreatment is taken, with S-3400N II type scanning electron microscopies（Japan
HITACHI is produced）Observed, amplification is 1500 times.
Test example：Illustrate the composite microbial system built with method of the present invention to be had below by way of test example
Beneficial effect, composite microbial system is accessed into 30 DEG C of cultures in described culture medium in this test example.Made with natural packing pretreatment
For control, growth of microorganism amount, pH value, COD value, scanning electron microscope image and wood fibre cellulose content is determined respectively.
Test example 1：The change of microbial growth curve in preprocessing process
OD during stalk pretreatment600The change of value as shown in figure 1, wherein, horizontal axis plots time in Fig. 1（d）, longitudinal axis representative
OD600Value, curve orbicular spot represent natural packing pretreatment, and square point represents compound bacteria pretreatment.In compound bacteria preprocessing process
0 ~ 2d is lag phase, and thalline increasess slowly；2 ~ 6d is increased logarithmic phase；6 ~ 10d be stable phase, material OD600Value be in all the time compared with
High level, reaches maximum 1.521 in 8d；Decline phase, material OD is entered after 10d600Value is slow to be reduced.Stalk pretreatment mistake
OD in journey600Value is all presented and first increases the trend for reducing afterwards, and in compound bacteria preprocessing process, microorganism lag phase is shorter, is illustrated certainly
So in banking process, the breeding of microorganism is slower, and compound bacteria can Adaptable growth environment faster, enable straw when shorter
It is interior to begin to degraded；Compound bacteria OD in preprocessing process600Value is consistently higher than natural packing pretreatment, illustrates that compound bacteria is located in advance
Than larger, thalli growth metabolism is more vigorous for cell concentration during reason straw.
Test example 2：The change of pH value in preprocessing process
During stalk pretreatment the change of pH value as shown in Fig. 2 wherein, horizontal axis plots time in Fig. 2（d）, the longitudinal axis represents pH
Value, curve orbicular spot represent natural packing pretreatment, and square point represents compound bacteria pretreatment.Compound bacteria pretreatment starts in fermentation
After rapidly enter the acidifying phase, pH value reaches minima 5.18 in 2d；PH value quickly goes back up to 6.81 in 2 ~ 6d；Material after 6d
PH value slow elevated trend is presented always.After compound bacteria pretreatment, the pH value of straw is consistently higher than natural packing pretreatment,
And straw experiences the longer acidifying phase in natural packing preprocessing process, is unfavorable for the growth of microorganism；Jing compound bacteria is located in advance
Material after reason can return to neutral level after 6d substantially.General non-methanogen to the subject range of acid-base value compared with
Extensively, and methanogen is extremely sensitive to environmental change, could normal growth metabolism only in the environment of pH 6.8 ~ 7.5.It is compound
Bacterium has stronger pH abilities of regulation and control during straw degradative, from the angle analysis of pH value, pretreated through compound bacteria
Straw is more suitable for follow-up anaerobic fermentation.
Test example 3：The change of COD in preprocessing process
During stalk pretreatment the change of COD value as shown in figure 3, wherein, horizontal axis plots time in Fig. 3（d）, longitudinal axis representative
COD（mg·L-1）, curve orbicular spot represents natural packing pretreatment, and square point represents compound bacteria pretreatment.Straw compound bacteria is pre-
COD value in processing procedure is presented in 0 ~ 8d and increases trend, reaches 12865 mg L of maximum in 8d-1；Experience peak period
Afterwards, the COD value of material starts slow reduction, and finally tend towards stability state.Found by Data Comparison, with preprocessing process
Carry out, in the pretreated material of Jing compound bacteria, the content of organic matter is raised, and illustrates that degraded of the compound bacteria to straw is progressively carried out, product
A large amount of organic matters are tired out；Subsequently reduce again, microorganism is illustrated during stalk pretreatment while consuming part organic matter；
Content of organic matter variation tendency in straw natural packing preprocessing process, illustrate straw in hydrolytic process degradation rate compared with
Slowly, degrade not complete enough.In preprocessing process, not exclusively, overlong time Organic substance can be consumed time short then straw degradative,
And the Organic substance produced after straw degradative is the carbon source in follow-up anaerobic fermentation process, analyze from the purpose using Organic substance, Jing
After compound bacteria pretreatment 8d, dissolved organic matter content reaches peak, is relatively adapted to anaerobic fermentation.
Test example 4：Straw structure change is analyzed
Before and after pretreatment, as Figure 4-Figure 6, the straw surfacing before pretreatment is smooth, compact structure for straw scanning electron microscope image
It is regular.The pretreated straw knot of natural packing constitutes bulk, and part-structure surface stratum disjunction occurs, illustrates that natural packing is pre-
The structure of energy changing section lignocellulose is processed, but degradation efficiency need to be improved；The chip residue of body structure surface is reduced, and
There is a little gap and hole, small particle straw is more first degraded in illustrating preprocessing process, to straw structural damage act on compared with
It is little.The straw rough surface, bulk Jing after compound bacteria pretreatment, there is a large amount of projections and hole in the surface of coarse structure, illustrate multiple
Close bacterium and destructive effects can be produced to surface integument by secreting digestive enzyme；Lignocellulosic structure changes, and increased
The exposed area of cellulose, is conducive to microorganism mycelia to be directly entered inside straw, by secreting digestive enzyme to internal wooden fibre
Dimension element is degraded.As a result show, during compound bacteria pretreated straw, improve the accessibility of lignocellulose, be conducive to
Improve the utilization ratio of lignocellulose.
Test example 5：The change of wood fibre cellulose content before and after pretreatment
Before and after pretreatment, the change of wood fibre cellulose content as shown in table 2, is significantly reduced in straw during stalk pretreatment
The content of neutral detergent fiber, cellulose and hemicellulose（P<0.05）, illustrate neutral detergent fiber, fiber in corn straw
Element and hemicellulose are easier degraded；Compare with natural packing pretreatment, corn straw in compound bacteria preprocessing process
Acid detergent fiber and content of lignin are significantly reduced（P<0.05）, illustrate that compound bacteria has to acid detergent fiber and lignin
Stronger degradation capability.
The chemical composition of corn straw before and after 2 pretreatment of table
| Corn straw | Neutral detergent fiber/% | Acid detergent fiber/% | Cellulose/% | Hemicellulose/% | Lignin/% |
| It is untreated | 73.76±0.39a | 45.83±0.14a | 36.53±1.27a | 27.93±1.62a | 15.38±0.92a |
| Natural packing pretreatment | 65.38±1.12b | 43.02±0.54a | 31.17±1.52b | 22.36±0.58b | 13.46±1.35a |
| Compound bacteria pretreatment | 54.13±2.08c | 38.61±1.05b | 23.78±0.63c | 15.52±0.76c | 9.35±1.21b |
Note：Numeral of going together in table difference lowercase letter indication difference is notable（P<0.05）.
The above, only presently preferred embodiments of the present invention not makees any formal and substantial limit to the present invention
System, all those skilled in the art, in the range of without departing from technical solution of the present invention, when using disclosed above
Technology contents, and few modifications, modification and the equivalent variations for developing made, are the Equivalent embodiments of the present invention；Meanwhile, it is all
According to change, modification and differentiation of the substantial technological of the present invention to any equivalent variations made for any of the above embodiments, still fall within
In the range of technical scheme.
Claims (6)
1. a kind of composite microbial system of fast degradation corn straw, it is characterised in that：
Composite microbial system includes funguses, antibacterial and actinomycetes, and wherein funguses include gloeophyllum trabeum, Phanerochaete chrysosporium, variegated cloud
Sesame, Trichoderma viride and aspergillus niger, antibacterial include Bacillus circulans, Pseudomonas aeruginosa, and actinomycetes include Streptomyces badius.
2. a kind of preparation method of composite microbial system as claimed in claim 1, it is characterised in that comprise the steps：
S1）Prepare fluid medium：Wherein, fluid medium include nutrient culture medium, LB culture medium, PDA culture medium with
And yeast powder, starch culture-medium；
S2）Single culture amplification culture：Each fluid medium to preparing carries out high temperature sterilize, after being cooled to room temperature, with connecing
Kind ring takes a small amount of each strain and carries out spawn culture；
S3）Generate composite microbial system：Each strain of slant preservation is activated by solid state rheology respectively, the difference after activation
Single culture is inoculated into different liquids culture medium respectively, and places it in culture in isothermal vibration incubator；Then by different bacterium
The bacterium solution mix homogeneously planted.
3. the preparation method of composite microbial system according to claim 2, it is characterised in that：
Prepare nutrient culture medium：Take 5 grams of peptone, 30 grams of Carnis Bovis seu Bubali cream, 5 grams of Sodium Chloride, 1000 milliliters of distilled water, 121
DEG C sterilizing 30 minutes, for the culture of Bacillus circulans；
Prepare LB culture medium：Take 5 grams of yeast extract, 10 grams of peptone, 10 grams of Sodium Chloride, 1000 milliliters of distilled water, at 121 DEG C
Sterilizing 30 minutes, for the culture of Pseudomonas aeruginosa；
Prepare yeast powder, starch culture-medium：Take 2 grams of yeast extract, 10 grams of soluble starch, 1000 milliliters of distilled water, 121
DEG C sterilizing 30 minutes, for the culture of Streptomyces badius；
Prepare PDA culture medium：1000 milliliters of Rhizoma Solani tuber osi extracting solution is taken, 20 grams of glucose sterilizes 30 minutes at 115 DEG C, for Huang
The flat lead fungi of archespore hair, Coriolus Versicolor, Trichoderma viride, aspergillus niger, the culture of gloeophyllum trabeum.
4. the preparation method of the composite microbial system according to Claims 2 or 3, it is characterised in that：
Single culture amplification culture refers to the culture medium that will be prepared, and sterilizes 30 minutes at high temperature, after being cooled to room temperature, with connecing
Plant ring and 1～2 ring of strain is taken in triangular flask, and place it in isothermal vibration incubator, 120r/min under 30 DEG C of constant temperatures
Continuous culture 2 days.
5. the preparation method of composite microbial system according to claim 4, it is characterised in that：
Generation composite microbial system is referred to and is activated each strain of slant preservation respectively by solid state rheology, the difference after activation
Single culture is inoculated into different liquids culture medium respectively, and places it in isothermal vibration incubator, under 30 DEG C of constant temperatures
120r/min is continuously cultivated 2 days, then by the bacterium solution mix homogeneously of different strain.
6. the preprocess method of the composite microbial system fast degradation corn straw described in a kind of utilization claim 1-5, its feature exist
In：
30 grams of straw is taken, is placed in 250 milliliters of triangular flasks, according to solid-liquid mass ratio 1：5 add bacterium solution, with sealing after mix homogeneously
Film is sealed, to maintain the moisture content of triangular flask mesostroma；Cultivate 14 days under 30 DEG C of constant temperatures, humid control is more than 80%.
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| CN201611034330.8A CN106520615A (en) | 2016-11-16 | 2016-11-16 | Composite microbial system for quickly degrading maize straw and preparation and pretreatment method thereof |
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| CN107619797A (en) * | 2016-07-21 | 2018-01-23 | 济南航晨生物科技有限公司 | One kind is based on solid waste mixed fermentation composite bacteria agent and its production technology |
| CN108191550A (en) * | 2018-03-06 | 2018-06-22 | 河北百禾丰化肥有限公司 | A kind of multi-element biological organic soil modified form fertilizer and preparation method thereof |
| CN108384738A (en) * | 2018-04-28 | 2018-08-10 | 华中农业大学 | One Pseudomonas aeruginosa strain and its screening technique and its application in stalk lactic fermentation |
| CN109385455A (en) * | 2018-12-06 | 2019-02-26 | 齐鲁工业大学 | A kind of application of wheat stalk hemicellulose |
| CN109805158A (en) * | 2019-01-16 | 2019-05-28 | 中国农业科学院北京畜牧兽医研究所 | A kind of method and application using fungal biodegradation corn stalk fiber |
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| CN110922975A (en) * | 2019-12-23 | 2020-03-27 | 南京朴厚生态科技有限公司 | Preparation method and application of microbial straw degradation microbial inoculum |
| CN110938574A (en) * | 2019-12-27 | 2020-03-31 | 黑龙江省农业科学院耕作栽培研究所 | Corn straw decomposition microbial inoculum |
| CN112746037A (en) * | 2020-12-16 | 2021-05-04 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
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| CN108191550A (en) * | 2018-03-06 | 2018-06-22 | 河北百禾丰化肥有限公司 | A kind of multi-element biological organic soil modified form fertilizer and preparation method thereof |
| CN108384738A (en) * | 2018-04-28 | 2018-08-10 | 华中农业大学 | One Pseudomonas aeruginosa strain and its screening technique and its application in stalk lactic fermentation |
| CN109385455A (en) * | 2018-12-06 | 2019-02-26 | 齐鲁工业大学 | A kind of application of wheat stalk hemicellulose |
| CN109805158A (en) * | 2019-01-16 | 2019-05-28 | 中国农业科学院北京畜牧兽医研究所 | A kind of method and application using fungal biodegradation corn stalk fiber |
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| CN110627543B (en) * | 2019-10-30 | 2021-11-12 | 江苏省农业科学院 | Method for promoting compost maturity of livestock and poultry manure through microbial pretreatment |
| CN110922975A (en) * | 2019-12-23 | 2020-03-27 | 南京朴厚生态科技有限公司 | Preparation method and application of microbial straw degradation microbial inoculum |
| CN110938574A (en) * | 2019-12-27 | 2020-03-31 | 黑龙江省农业科学院耕作栽培研究所 | Corn straw decomposition microbial inoculum |
| CN112746037A (en) * | 2020-12-16 | 2021-05-04 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
| CN113817639A (en) * | 2021-09-26 | 2021-12-21 | 黑龙江省农业科学院耕作栽培研究所 | Efficient corn straw degradation composite microbial inoculum, and preparation method and application thereof |
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