CN106511547A - Medicine for preventing and treating frog disease in America and preparation method of medicine - Google Patents

Abstract

The invention discloses a medicine for preventing and treating frog disease in America. The medicine is prepared from, by mass, 170-210 parts of rheum officinale, 50-80 parts of concha haliotidis, 190-220 parts of alfalfa, 180-215 parts of milkvetch roots, 180-210 parts of myrrh, 80-110 parts of wormwood, 130-165 parts of salvia chinensis, 185-215 parts of cape jasmine, 105-135 parts of asarum, 105-140 parts of gleditsia officinalis, 135-160 parts of white paeony roots, 120-150 parts of Chinese fevervine herb and roots and 50-70 parts of cassia seeds. According to the medicine, incompatibility of the raw materials in the medicine does not exist, the occurrence rate of frog disease in America can be obviously lowered, a quite good prevention and treatment effect is achieved on frog disease in America, no toxic or side effect is produced, use is safe, and no harmful medicine residue is left.

Description

A kind of protective agents of Rana grylia disease and preparation method thereof

Technical field

The invention belongs to Rana nigromaculata cultural technique field, and in particular to a kind of protective agents of Rana grylia disease and its preparation Method.

Background technology

Rana grylia also known as the river frog, Hylarana guentheri.The U.S. is originated in, the later stage eighties 20th century introduces China, its fine and tender taste, Delicious flavour, is first-class Rana esculenta, has promoted cultivation throughout the country.Rana grylia has that individual big, growth is fast, taste Delicious, protein content is high, is a kind of high-quality and efficient economic cultivation batrachia.With industry competition fierceness, some old raisers Cultural technique old stuff, causes to produce effect lowly, and some can't regain one's original capital, and some cultivation are not stinted using some forbidden drugs, cause Rana nigromaculata Harmful substance accumulation, so as to cause mortality, also makes cultivation water suffer severe contamination in vivo, also ecological around collateral damage Environment.

In breeding process, large-scale cultivation is susceptible to disease to Rana grylia, and is very easy to spread, mainly There is following disease：Tadpole period Common Diseases：Disease, septicemia, enteritis, fish moldss, ascites, trichodinasis, gas bubble disease；It is common into the frog Disease：Enteritis, red leg disease, infectious liver disease, white-eyes-sickness, bark rot etc., by putting into practice, exploring, Experimental comparison, take to prevent Based on.

Wherein Rana grylia torticollis disease is that the bacterium directly destroys the cranial nerve of the frog by caused by meningitiss deteriorated blood Flavobacterium, Cause mentally deranged generation torticollis.The ill frog is ceaselessly spinned in water, askew turn to the left or to the right of its head.Sick frog food ration subtracts Few, mortality rate is high, if processing the sick frog not in time, it will infect frog group.Main infection mode has two kinds, and one is directly from parent To filial generation, two is mutually infection.

Rana grylia disease is prevented and treated using Western medicine typically in prior art, drug dependence is easily caused, or is affected Rana nigromaculata Meat, it is therefore necessary to develop a kind of protective agents of Rana grylia disease.

The content of the invention

It is an object of the invention to overcome the deficiencies in the prior art, there is provided a kind of protective agents of Rana grylia disease, medicine In thing there is no incompatibility in each material combination, can substantially reduce the incidence rate of Rana grylia disease, Rana grylia disease is had Good preventive and therapeutic action, and have no toxic side effect, using safety, remain without hazardous drugs.

The technical solution adopted for the present invention to solve the technical problems is：

The technical scheme is that：A kind of protective agents of Rana grylia disease, which is by the raw material of following masses part proportioning Medicine is made：Radix Et Rhizoma Rhei 170-210 parts, Concha Haliotidis 50-80 parts, toothed burclover 190-220 parts, Radix Astragali 180-215 parts, Myrrha 180-210 parts, Folium Artemisiae Argyi 80-110 parts, Herba Salviae Chinensiss 130-165 parts, Fructus Gardeniae 185-215 parts, Herba Asari 105-135 parts, Fructus Gleditsiae Abnormalis 105-140 parts, Radix Paeoniae Alba 135- 160 parts, Herba Paederiae 120-150 parts, Semen Cassiae 50-70 parts.

Its further preferred mass part proportioning is：Radix Et Rhizoma Rhei 170-205 parts, Concha Haliotidis 50-75 parts, toothed burclover 190-215 parts, Radix Astragali 190-215 parts, Myrrha 185-210 parts, Folium Artemisiae Argyi 80-105 parts, Herba Salviae Chinensiss 130-160 parts, Fructus Gardeniae 185-210 parts, Herba Asari 105- 130 parts, Fructus Gleditsiae Abnormalis 110-140 parts, Radix Paeoniae Alba 140-160 parts, Herba Paederiae 125-150 parts, Semen Cassiae 50-70 parts.

Its further preferred mass part proportioning is：Radix Et Rhizoma Rhei 180-205 parts, Concha Haliotidis 50-70 parts, toothed burclover 190-210 parts, Radix Astragali 190-210 parts, Myrrha 190-210 parts, Folium Artemisiae Argyi 90-105 parts, Herba Salviae Chinensiss 140-160 parts, Fructus Gardeniae 190-210 parts, Herba Asari 110- 130 parts, Fructus Gleditsiae Abnormalis 120-140 parts, Radix Paeoniae Alba 145-160 parts, Herba Paederiae 130-150 parts, Semen Cassiae 50-70 parts.

Its further preferred mass part proportioning is：Radix Et Rhizoma Rhei 190-205 parts, Concha Haliotidis 55-70 parts, toothed burclover 190-205 parts, Radix Astragali 195-210 parts, Myrrha 190-205 parts, Folium Artemisiae Argyi 95-105 parts, Herba Salviae Chinensiss 140-155 parts, Fructus Gardeniae 190-205 parts, Herba Asari 110- 125 parts, Fructus Gleditsiae Abnormalis 120-135 parts, Radix Paeoniae Alba 145-155 parts, Herba Paederiae 135-150 parts, Semen Cassiae 50-70 parts.

Its further preferred mass part proportioning is：Radix Et Rhizoma Rhei 190-200 parts, Concha Haliotidis 60-70 parts, toothed burclover 190-200 parts, Radix Astragali 200-210 parts, Myrrha 190-200 parts, Folium Artemisiae Argyi 100-105 parts, Herba Salviae Chinensiss 150-155 parts, Fructus Gardeniae 200-205 parts, Herba Asari 110-120 parts, Fructus Gleditsiae Abnormalis 130-135 parts, Radix Paeoniae Alba 145-150 parts, Herba Paederiae 140-150 parts, Semen Cassiae 60-70 parts.

Its further preferred mass part proportioning is：200 parts of Radix Et Rhizoma Rhei, 60 parts of Concha Haliotidis, 200 parts of toothed burclover, 200 parts of the Radix Astragali, 200 parts of Myrrha, 100 parts of Folium Artemisiae Argyi, 150 parts of Herba Salviae Chinensiss, 200 parts of Fructus Gardeniae, 120 parts of Herba Asari, 130 parts of Fructus Gleditsiae Abnormalis, 150 parts of the Radix Paeoniae Alba, Herba Paederiae 140 parts, 60 parts of Semen Cassiae.

The primary raw material medicine that the present invention is used is described as follows：

Radix Et Rhizoma Rhei:Radix Et Rhizoma Rhei is being collectively referred to as the perennial plant of various Polygonaceae Rheums, and the title of Chinese crude drug.Radix Et Rhizoma Rhei of the present invention refers to Be todaiwo,rhizoma.Autumn Mo stem and leaf is withered or the front excavation of secondary spring germination.Radicula is removed, crust, valve cutting or section is scraped off, rope wears bunchiness It is dried or convection drying, Chinese herb rhubarb has attacks the effects such as stagnant, clearing away damp-heat, pathogenic fire purging, removing heat from blood, blood stasis dispelling, removing toxic substances.

Concha Haliotidis：For the shell of Bao Ke animal Haliotis diversicolors, haliotis discus hannai Ino, ear Bao, sheep Bao etc..Also known as natural pearls are female（《Thunder God big gun Process opinion》）, Carnis Haliotidiss first（《Collective Notes to Canon of Materia Medica》）, nine hole spiral shells（《Japan hanako materia medica》）, Herba Senecionis Scandentiss（《Detailed outline》）, very sea certainly, Hai Jue Bright, CONCHA HALIOTIDI, abalone shells, nine hole Concha Haliotidis（《Pharmacognosy》）, Carnis Haliotidiss skin（《Shandong Chinese medicine handbook》）, golden Carnis Mactrae skin（《In Shandong Medical herbs》）.There are calming liver and clearing heat, effect of shade removing.This product is conventional Chinese medicine, and " Mingyi Bielu " is classified as top grade.Modern commercially available product point Determine for light bottom sea, hair bottom sea is certainly determined three kinds with sea.Original animal is determined for haliotis diversicolor Reeve in light bottom sea, and the extra large former animal of determining in hair bottom is big for disk Bao, sea former animal certainly are sheep Bao.Other title natural pearls are female, Carnis Haliotidiss first, nine hole spiral shells, Herba Senecionis Scandentiss, Carnis Haliotidiss skin, golden Carnis Mactrae skin.

Toothed burclover:It is the address of the people to Herba Loti Tenuis on the ground such as Shanghai, granary, Changshu.Herba Loti Tenuis, original name south lucerne vegetables MedicagohispidaGaertn has Ye Sanchi, such as reversed cardioid, tip slightly circle or recessed top to have sawtooth, and the surface of leaf is in dense Green, stalk are extremely short, and main root is long, multi-branched.Stem is generally vertical, closely without hair, high 30-100 centimetre.Compound leaf has 3 lobules, lobule Obovate or lanceolar, long 1-2 centimetre, wide about 0.5 centimetre, nose-circle, middle rib slightly protrude, and first half leaf has sawtooth, base portion Narrow wedge shape；Stipule narrow lanceolar, full edge.Raceme axil is given birth to, flower 8-25.Pod spiral type, stingless, top has point to expose nozzle； The production of two province of Jiangsu and Zhejiang Provinces is special more.Toothed burclover is leguminous plant, Jing often to eat toothed burclover element can balanced human pH-value.

The Radix Astragali：Also known as continuous stilbene.Perennial herb is high 50-100 centimetre.Main root is plump, wooden, normal branch, canescence.Stem Uprightly, top multi-branched, has thin rib, by white pubescence.Perennial herb is high 50-100 centimetre.Originate in Inner Mongol, Shanxi, sweet The ground such as respectful, Heilungkiang.The medicinal history for having more than 2000 years so far of the Radix Astragali, which has enhancing human body immunity function, liver protection, profit Urine, defying age, anti-stress, blood pressure lowering and wide antibacterial action.But table excess pathogen contain, retention of dampness due to stagnation of QI, food stagnation stagnate, carbuncle from the beginning of Or the excess syndrome such as pyretic toxicity Shang Sheng after bursting, and hyperactivity of YANG due to deficiency of YIN person, clothes must be prohibited.

Myrrha：Chinese medicine name, is olive subject plant Herba Violae tree CommiphoramyrrhaEngl. or Ha Didingshu CommiphoramolmolEngl. dry resin.It is divided into natural Myrrha and gum opoponax.It is distributed in Somalia, Ethiopia Russia ratio The ground such as sub- and Arabia Peninsula south.The effect of with scattered stasis pain, detumescence and promoting granulation.It is usually used in obstruction of qi in the chest and cardialgia, gastralgia, pain The treatment of the diseases such as Jing amenorrhea, postpartum stagnation, abdominal mass stomachache, rheumatic arthralgia, traumatic injury, carbuncle swells sore.

Folium Artemisiae Argyi：Perennial herb or slightly into suffruticose, plant has enhanced aroma.Stem list is given birth to or minority, brown or sallow Brown, base portion slightly lignifying, top Bi matter, and the branch for having minority short, leaf ground paper matter, above by canescence pubescence, base portion Generally without pseudostipule or minimum pseudostipule；Upper leaf and bract leaf pinniform are partly split, head inflorescence ellipse, corolla tubulose or height Foot cup-shaped, outside have gland point, and flower pesticide is narrow linear, and style and corolla be near isometric or slightly longer than corolla.Achene long avette or Long Circle. The flowering fruit bearing stage 9-10 months.All herbal medicine, have warming the meridian, dry, cold expelling, hemostasis, antiinflammatory, relieving asthma, cough-relieving, the effect such as antiabortive, antiallergic. Folium Artemisiae Argyi is dried and smashs to pieces " Folium Artemisiae Argyi ", and moxa roll processed can make the raw material of " ink paste " again for moxa-moxibustion.

Herba Salviae Chinensiss：Also known as：Dali Radix Rubiae, Rubiayunnanensis (Franch.) Dlels, Arisaema balansae Engl., dice piece etc., genus rubia mesh, Rubiaceae draft；Indication： Heat-clearing and toxic substances removing, blood circulation promoting regulates qi pain relieving.Swell for acute, chronic hepatitis, gastral cavity side of body distending pain, damp-heat vaginal discharge, mastitis, furuncle.

Fructus Gardeniae：Another name Yellow Fructus Gardeniae, Fructus Gardeniae, white toad, Flos Gardeniae, are the fruits of Maguireothamnus speciosus Fructus Gardeniae.Genus rubia section, Fructus Gardeniae Category, evergreen shrubss；Root, leaf, fruit can be used as medicine, and have the effect of purging intense heat relieving restlessness, clearing away heat and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body；Effect：Clearly Lung cough-relieving, cooling blood for hemostasis, pathogenic fire purging cure mainly cough due to lung-heat, cough up phlegm, toxic swelling, control calentura restlessness of asrhenia type and insomnia, and jaundice, gonorrhea are quenched one's thirst, mesh Red, pharyngalgia is spitted blood, and epistaxis, dysentery, hematuria, pathopyretic ulcer are sprained and swollen and ache.《Dictionary of medicinal plant》Described in：Fructus Gardeniae, different name：Wood Red (《This Jing》), fresh (《Upper woods is assigned》), ancient wine vessel (《Chinese book》Meng Kang note), more Fructus Persicae (《Do not record》), stand (《Collective Notes to Canon of Materia Medica》), SHANZHIZI (《Haigoushen》), Fructus Gardeniae (《Newly Revised Canon of Materia Medica》), it is little danger son (《Book on Chinese herbal medicine is original》), Huang sprout (《Fujian east book on Chinese herbal medicine》), yellow Cape jasmine Son (《Jiangsu medical material will》), yellow Cape jasmine, gardenia jasminoides ellis, Fructus Gardeniae (《Zhejiang medicinal plants will》)；For Rubiaceae Fructus Gardeniae platymiscium Fructus Gardeniae Fruit.

Herba Asari；Another name：Asarum sieboldii, basin grass Herba Asari, Aristolochiaceae, asarum perennial herb；Herba Asari as medicinal plants, Shi Zai Qin Han《Sheng Nong's herbal classic》,《Sheng Nong's herbal classic》It is classified as top grade.Herba Asari root is thin, color sallow, leaf color are green, and acrid in the mouth is peppery Numb tongue.It is warm in nature, it is slightly poisonous.GUIXIN, lung, kidney channel.There are wind-dispelling, cold expelling, warming the lung, water-filling, the effect having one's ideas straightened out.Control headche due to wind-cold, nose Deep pool, toothache, phlegm retention cough with dyspnea, rheumatic arthralgia.Additionally, Herba Asari also have anti inflammatory immunity, Herba Asari local anesthesia, improve metabolism, Antibacterial action, also can be used for the leukopenia of Chemotherapy of Tumor Patients and caused by radiation.

Fructus Gleditsiae Abnormalis：Also known as：Fructus Gleditsiae Abnormalis.It is the small-sized fruit tied after leguminous plant Fructus Gleditsia plant injury, bends to crescent. The flat elder of shape, referred to as big Fructus Gleditsia（That is Fructus Gleditsia）.Containing triterpenoid saponin, tannin, ceryl alcohol, nonacosane, stigmasterol etc..There is eliminating the phlegm, urge Tell, the effect such as diarrhea inducing.

The Radix Paeoniae Alba：Also referred to as Paeonia sterniana Fletcher in Journ., is Ranunculaceae Radix Paeoniae platymiscium.In the existing long cultivation history of China, in being renown far and wide Outward, which claims Paeonia sterniana Fletcher in Journ..Its root can be used as medicine.Smooth liver and relieve pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating.For dizziness of having a headache, hypochondriac pain, suffer from abdominal pain, Limb pain twin, blood deficiency and yellow complexion, menoxenia, spontaneous perspiration, night sweat.

Herba Paederiae：Herba Paederiae refers generally to fevervine, is the herb of Maguireothamnus speciosus fevervine, summer harvesting herb, dries. Its sweet in the mouth, slight bitter, it is mild-natured.The effect of with expelling wind and removing dampness, analgesic antidotal, promoting digestion and removing stagnation, promoting blood circulation and detumescence.For rheumatism muscles and bones Bitterly, traumatic injury, traumatic pain, liver and gall and stomach intestinal colic, dyspepsia, infantile malnutrition, bronchitis；It is used for outward dermatitis, Eczema and sore swollen toxin.

Semen Cassiae：The dry mature seed of leguminous plant Semen Cassiae or little Cassia tora, with its have improving eyesight work(and name it.Autumn Harvesting mature fruit, dries, and lays seed, removes impurity.Semen Cassiae bitter in the mouth, sweet, salty, cold nature, enter liver, kidney, large intestine channel；Profit Intestinal relieving constipation, lipid-loweringing improving eyesight, treatment constipation and hyperlipidemia, hypertension.Liver heat removing and eyesight improving, diuretic relieving constipation have laxative action, blood pressure lowering drop Blood fat.

In the medicine of the present invention there is no incompatibility in each material combination, can substantially reduce the generation of Rana grylia disease Rate, has good preventive and therapeutic action to Rana grylia disease, and has no toxic side effect, using safety, residual without hazardous drugs Stay.

It is a further object of the present invention to provide a kind of preparation method of said medicine, its process is simple, its crude drug is common, Cheaply.

The preparation method of medicine of the present invention is as follows：

The first step:The raw material weight of batch production is calculated by formula proportion；

Second step:The raw material for calculating batch production is positioned in stirring container, be soaked in water 3-5 hours, wherein the use of water Measure 30 times for Chinese medicine gross weight；Warm fire heating is burnt to seething with excitement, and endures 15-20 minutes, and after being then incubated 3 hours, then warm fire heating is burnt To seething with excitement, 25-30 minutes are endured；

3rd step:Carry out centrifugal filtration, filtrate after 2-3 hours precipitation, second centrifugal filtration；Filtrate is this The medicine of bright preventing and treating Rana grylia disease.

The medicine using method of the present invention：Into the frog phase, when feeding every time, every 10 kg diet weighs 1000 grams of U.S. green grass or young crops The protective agents filtrate of frog disease, adds 50 grams of brown sugar in Chinese medicine composition filtrate, is subsequently adding in feedstuff, mixes, Feed immediately, sooner or later each daily to feed once, free choice feeding, 10 days is a course for the treatment of, can feed this medicine one every 10 days The individual course for the treatment of, froglet phase medicine halve.

In order to be illustrated more clearly that the present invention, following examples are enumerated, but its scope to invention is without any restrictions.

Specific embodiment

Embodiment 1

Weigh Radix Et Rhizoma Rhei 200g, Concha Haliotidis 60g, toothed burclover 200g, Radix Astragali 200g, Myrrha 200g, Folium Artemisiae Argyi 100g, Herba Salviae Chinensiss 150g, Fructus Gardeniae 200g, Herba Asari 120g, Fructus Gleditsiae Abnormalis 130g, Radix Paeoniae Alba 150g, Herba Paederiae 140g, 60 parts of Semen Cassiae, these material compositions are through shining Quality after dry；

The first step:The raw material weight of batch production is calculated by formula proportion；

Second step:The raw material for calculating batch production is positioned in stirring container, is soaked in water 4 hours, wherein the consumption of water For 30 times of Chinese medicine gross weight；Warm fire heating is burnt to boiling, endures 20 minutes, and after being then incubated 3 hours, then warm fire heating is burnt to boiling Rise, endure 30 minutes；

3rd step:Carry out centrifugal filtration, filtrate after 2 hours precipitations, second centrifugal filtration；Filtrate is the present invention The medicine of preventing and treating Rana grylia disease.

Embodiment 2-14

Embodiment 2-12 in addition to raw material mass mixture ratio is different from embodiment 1, its raw material type and step of preparation process and use Method is same as Example 1, and comparative example 1 is that Myrrha is eliminated on the basis of embodiment 1, and comparative example 2 is the base in embodiment 1 Herba Salviae Chinensiss are eliminated on plinth, raw material that embodiment 1-12, comparative example 1-2 are used and quality as shown in Table 1 below, table below 1 shows the raw material and quality used by embodiment 1-12, comparative example 1-2.

Table 1

Clinical trial one：

(1) laboratory animal

Select 300 Rana grylias with torticollis disease into the frog, loss of appetite, be reluctant activity；

(2) experimental technique

It is random to be grouped, 15 groups are divided into, 20 per group, wherein 14 groups is experimental group, embodiment 1-12, comparative example 1- is fed respectively The feedstuff that medicine described in 2 is made into, daily 2 times, free choice feeding, continuous 10 days；Another group is matched group, using prior art sulfanilamide Pyrimidine therapy, feeds according still further to normal feed mode.Treatment is observed and is made a record, be specifically shown in Table 2 for 10 days afterwards；

(3) standard and observational technique are treated

Cure：Torticollis disease recovery from illness；

Effectively：Torticollis disease is effectively alleviated, and has very big alleviation；

It is invalid：Torticollis disease is not effectively alleviated.

Table 2

	Cure number	Significant figure	Invalid number
				Embodiment 1	20	0	0
Embodiment 2	19	1	0
				Embodiment 3	20	0	0
Embodiment 4	19	1	0
				Embodiment 5	19	1	0
Embodiment 6	18	1	1
				Embodiment 7	19	1	0
Embodiment 8	18	1	1
				Embodiment 9	19	1	0
Embodiment 10	19	1	0
				Embodiment 11	20	0	0
Embodiment 12	19	1	0
				Comparative example 1	12	5	3
Comparative example 2	12	5	3
				Matched group	10	2	8

It follows that in the case of equal consumption, it is right that the therapeutic effect being mixed into using medicine of the present invention in feedstuff is substantially better than Ratio and matched group.And after 1 month, experimental group is 0 recurrence, and matched group has 4 recurrences.

Clinical trial two：

In order to prove feeding effect of the present invention to Rana grylia, feeding experiment is repeatedly tried, choose large-scale Rana grylia and support Field 3000 is grown in the Rana grylia into the frog phase, body weight size, health status are basically identical, are randomly divided into 15 groups, per group 200 Only.Wherein 14 groups is experimental group, the feedstuff that feeding embodiment 1-12, medicine described in comparative example 1-2 are made into respectively, twice daily, Free choice feeding, continuous 10 days；One course for the treatment of of this medicine was fed every 10 days.Another group is matched group, feeds and is not added with the identical of medicine Feedstuff, the nursing number of times of 15 groups of Rana nigromaculatas, feeding volume, nursing time all same.The various performance indications of 15 groups of Rana nigromaculatas are contrasted, averagely Value such as table 3.

After 1 month, 15 groups of Rana nigromaculata situation such as tables 3：

Table 3

	Ill evil number	Moon weightening (g)/only
			Embodiment 1	0	135
Embodiment 2	1	127
			Embodiment 3	0	129
Embodiment 4	0	129
			Embodiment 5	0	137
Embodiment 6	0	139
			Embodiment 7	0	129
Embodiment 8	0	129
			Embodiment 9	3	128
Embodiment 10	0	136
			Embodiment 11	0	130
Embodiment 12	0	132
			Comparative example 1	30	118
Comparative example 2	40	110
			Matched group	100	90

Can be evident that from table, after the medicine that the continuous feeding present invention is provided, daily gain is more right for experimental group Rana nigromaculata Increase obvious according to group, and experimental group is compared with matched group honey stomach, energetic, state is good, substantially no disease occurs.

So the present invention is not limited to the protective agents as Rana grylia disease, as long as composition of raw materials and phase of the present invention Same or equivalent medicine just falls within the scope and spirit of the invention.By the protective agents with the Rana grylia disease of the present invention The raw material of same recipe is made any Chinese medicine preparation and is also fallen within the scope and spirit of the invention, if that is, composition of raw materials with Identical medicine of the present invention just falls within the scope and spirit of the invention.

Claims (7)

1. a kind of protective agents of Rana grylia disease, it is characterised in that be made up of the crude drug of following masses part proportioning：Radix Et Rhizoma Rhei 170-210 parts, Concha Haliotidis 50-80 parts, toothed burclover 190-220 parts, Radix Astragali 180-215 parts, Myrrha 180-210 parts, Folium Artemisiae Argyi 80-110 Part, Herba Salviae Chinensiss 130-165 parts, Fructus Gardeniae 185-215 parts, Herba Asari 105-135 parts, Fructus Gleditsiae Abnormalis 105-140 parts, Radix Paeoniae Alba 135-160 parts, chicken droppings Rattan 120-150 parts, Semen Cassiae 50-70 parts.

2. protective agents of Rana grylia disease according to claim 1, it is characterised in that Radix Et Rhizoma Rhei 170-205 parts, Concha Haliotidis Bright 50-75 parts, toothed burclover 190-215 parts, Radix Astragali 190-215 parts, Myrrha 185-210 parts, Folium Artemisiae Argyi 80-105 parts, Herba Salviae Chinensiss 130-160 Part, Fructus Gardeniae 185-210 parts, Herba Asari 105-130 parts, Fructus Gleditsiae Abnormalis 110-140 parts, Radix Paeoniae Alba 140-160 parts, Herba Paederiae 125-150 parts, certainly Pine torch 50-70 parts.

3. protective agents of Rana grylia disease according to claim 1, it is characterised in that Radix Et Rhizoma Rhei 180-205 parts, Concha Haliotidis Bright 50-70 parts, toothed burclover 190-210 parts, Radix Astragali 190-210 parts, Myrrha 190-210 parts, Folium Artemisiae Argyi 90-105 parts, Herba Salviae Chinensiss 140-160 Part, Fructus Gardeniae 190-210 parts, Herba Asari 110-130 parts, Fructus Gleditsiae Abnormalis 120-140 parts, Radix Paeoniae Alba 145-160 parts, Herba Paederiae 130-150 parts, certainly Pine torch 50-70 parts.

4. protective agents of Rana grylia disease according to claim 1, it is characterised in that Radix Et Rhizoma Rhei 190-205 parts, Concha Haliotidis Bright 55-70 parts, toothed burclover 190-205 parts, Radix Astragali 195-210 parts, Myrrha 190-205 parts, Folium Artemisiae Argyi 95-105 parts, Herba Salviae Chinensiss 140-155 Part, Fructus Gardeniae 190-205 parts, Herba Asari 110-125 parts, Fructus Gleditsiae Abnormalis 120-135 parts, Radix Paeoniae Alba 145-155 parts, Herba Paederiae 135-150 parts, certainly Pine torch 50-70 parts.

5. protective agents of Rana grylia disease according to claim 1, it is characterised in that Radix Et Rhizoma Rhei 190-200 parts, Concha Haliotidis Bright 60-70 parts, toothed burclover 190-200 parts, Radix Astragali 200-210 parts, Myrrha 190-200 parts, Folium Artemisiae Argyi 100-105 parts, Herba Salviae Chinensiss 150-155 Part, Fructus Gardeniae 200-205 parts, Herba Asari 110-120 parts, Fructus Gleditsiae Abnormalis 130-135 parts, Radix Paeoniae Alba 145-150 parts, Herba Paederiae 140-150 parts, certainly Pine torch 60-70 parts.

6. protective agents of Rana grylia disease according to claim 1, it is characterised in that 200 parts of Radix Et Rhizoma Rhei, Concha Haliotidis 60 Part, 200 parts of toothed burclover, 200 parts of the Radix Astragali, 200 parts of Myrrha, 100 parts of Folium Artemisiae Argyi, 150 parts of Herba Salviae Chinensiss, 200 parts of Fructus Gardeniae, 120 parts of Herba Asari, Fructus Gleditsiae Abnormalis 130 parts, 150 parts of the Radix Paeoniae Alba, 140 parts of Herba Paederiae, 60 parts of Semen Cassiae.

7. the preparation method of the protective agents of Rana grylia disease according to claim 1, it is characterised in that including following step Suddenly:

The first step:The raw material weight of batch production is calculated by formula proportion；

Second step:The raw material for calculating batch production is positioned in stirring container, be soaked in water 3-5 hours, wherein the use of water Measure 30 times for Chinese medicine gross weight；Warm fire heating is burnt to seething with excitement, and endures 15-20 minutes, and after being then incubated 3 hours, then warm fire heating is burnt To seething with excitement, 25-30 minutes are endured；

3rd step:Carry out centrifugal filtration, filtrate after 2-3 hours precipitation, second centrifugal filtration；Filtrate is as prevented and treated The medicine of Rana grylia disease.