CN106511485A - Chinese herbal medicinal polysaccharide composition and application thereof - Google Patents
Chinese herbal medicinal polysaccharide composition and application thereof Download PDFInfo
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- CN106511485A CN106511485A CN201610957660.8A CN201610957660A CN106511485A CN 106511485 A CN106511485 A CN 106511485A CN 201610957660 A CN201610957660 A CN 201610957660A CN 106511485 A CN106511485 A CN 106511485A
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- panaxan
- wheat
- inokopolyose
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 60
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 60
- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 44
- 239000003814 drug Substances 0.000 claims abstract description 21
- 241000005787 Cistanche Species 0.000 claims abstract description 17
- 241000208340 Araliaceae Species 0.000 claims abstract description 15
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 15
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 15
- 235000008434 ginseng Nutrition 0.000 claims abstract description 15
- 238000009098 adjuvant therapy Methods 0.000 claims abstract description 7
- 241000209140 Triticum Species 0.000 claims description 61
- 235000021307 Triticum Nutrition 0.000 claims description 61
- 235000005911 diet Nutrition 0.000 claims description 61
- 230000037213 diet Effects 0.000 claims description 61
- 239000002131 composite material Substances 0.000 claims description 31
- 238000002360 preparation method Methods 0.000 claims description 26
- 235000013402 health food Nutrition 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 241000427159 Achyranthes Species 0.000 claims description 19
- 210000004985 myeloid-derived suppressor cell Anatomy 0.000 claims description 18
- 230000002265 prevention Effects 0.000 claims description 18
- 238000005352 clarification Methods 0.000 claims description 16
- 239000012141 concentrate Substances 0.000 claims description 15
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- 239000004480 active ingredient Substances 0.000 claims description 9
- 230000002829 reductive effect Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
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- 101100078001 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MSC2 gene Proteins 0.000 description 9
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- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 2
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- 235000013399 edible fruits Nutrition 0.000 description 1
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- LWYXLXAMDLNBFQ-UHFFFAOYSA-N iso-6-Carnavalin Natural products CC(O)CCCCCCCCCCC1CCC(O)C(C)N1 LWYXLXAMDLNBFQ-UHFFFAOYSA-N 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the field of health care food, in particular to a Chinese herbal medicinal polysaccharide composition and an application thereof. The Chinese herbal medicinal composition provided by the invention consists of herba cistanche, radix achyranthis bidentatae and ginseng or consists of polysaccharide of the herba cistanche, polysaccharide of the radix achyranthis bidentatae and polysaccharide of the ginseng. Experiments show that the Chinese herbal medicinal composition, medicines or health care food prepared by the invention is subjected to scientific compatibility, various components supplement each other and the prepared herbal medicinal composition, medicines or health care food is free from toxic and side effects. The Chinese herbal medicinal composition not only has a nutritive value but also recovers a normal immunological surveillance function of body; and an effect of preventing tumors and/or adjuvant therapy can be achieved.
Description
Technical field
The present invention relates to field of health care food, more particularly to a kind of polysaccharides of traditional Chinese medicine composition and application thereof.
Background technology
There is limitation in existing oncotherapy.Tumour occupy the first place of cause of the death spectrum at present, becomes great health threat, swells
Three big conventional therapies of knurl：Operation, chemicotherapy can be caused with certain limitation, and certain pain sensation is caused to patient
Receive, chemicotherapy also has different degrees of inhibitory action to normal immune system.
Immunization therapy is most important.With the progress of tumor immunology, it is increasingly recognised that immune system pair
In tumour monitoring and remove it is critical that, immunization therapy is described as being expected to the 4th class treatment method for capturing cancer.
Immune negative regulation cell becomes novel targets.In the past of concern is positive immunity, i.e., immunocyte is thin to tumour
The direct killing of born of the same parents, and now academia thinks, tumour cell realize immunologic escape be tumour be able to occur development it is important before
Carry, and the important biomolecule event is that an immune negative regulation network is there is in tumor microenvironment the reason for generation, in the network
Immune negative regulation cell and the factor inhibit monitoring and removing of the immune effector cell to tumour cell.Marrow source SC
(MDSC), regulatory T cells (Treg) are main immune negative regulation cells.
Polysaccharides of traditional Chinese medicine can improve tumor microenvironment.Polysaccharide is the main component of Chinese herbal medicine extract, and some Chinese herbal medicines are more
Sugar can regulate and control activation, propagation and the differentiation of immunocyte, and research in recent years shows that polysaccharides of traditional Chinese medicine is to immune negative regulation cell
Differentiation there is regulating and controlling effect, and then affect its function, performance improves the effect of tumor microenvironment.
The content of the invention
In view of this, the present invention provides a kind of polysaccharides of traditional Chinese medicine composition and application thereof.The Chinese herbal medicine group of gained of the invention
Compound, medicine or health food Jing scientific compatibilities are formed, and are brought out the best in each other, are had no toxic side effect.Both it is nutritious, there is recovery machine again
The normal immune surveillance function of body, can play prevention and/or auxiliary therapeutic action to tumour.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of herbal composite, is made up of saline cistanche, the root of bidentate achyranthes and ginseng.
In some specific embodiments of the present invention, described in the herbal composite, the active ingredient of saline cistanche is
Wheat-based diet, the active ingredient of the root of bidentate achyranthes is Inokopolyose, and the active ingredient of the ginseng is panaxan.
In some specific embodiments of the present invention, wheat-based diet described in the herbal composite, the ox
Knee polysaccharide is (1～5) with the mass ratio of the panaxan:(1～5):(1～5).
In some specific embodiments of the present invention, wheat-based diet described in the herbal composite, the ox
Knee polysaccharide is (2～4) with the mass ratio of the panaxan:(2～4):(2～4).
In some specific embodiments of the present invention, wheat-based diet described in the herbal composite, the ox
Knee polysaccharide is 2 with the mass ratio of the panaxan:3:3.
In some specific embodiments of the present invention, in the herbal composite, the wheat-based diet, the root of bidentate achyranthes are more
Sugar or panaxan comprise the following steps：Respectively by saline cistanche, the root of bidentate achyranthes or ginseng crushing after, in terms of g/mL, according to saline cistanche,
The root of bidentate achyranthes or people participate in the mass volume ratio of water and mix for the ratio of (0.5g～2.0g): 5mL, the heating stirring at 70 DEG C～75 DEG C
2～4h is extracted, filter residue is collected by filtration and is repeated to extract twice according to above-mentioned solid-liquid ratio, temperature and time, merge No. three extracts, Jing
Cross reduced pressure concentration and centrifugation obtains clarification concentrate, be 1 according to the volume ratio of clarification concentrate and 95% ethanol: (7～10) mix
Close, staticly settle in 4 DEG C, precipitation is collected by centrifugation, wheat-based diet, Inokopolyose or ginseng are obtained through vacuum freeze drying
Polysaccharide.
Present invention also offers the herbal composite prepare release MDSC inhibitor that T cell is bred and/or
Lower the application in the preparation of tumor mice Treg cell proportions.
Present invention also offers the herbal composite is in the health food and/or medicine for improving immunity function is prepared
Purposes.
Present invention also offers the herbal composite is in the health food for preparing prevention and/or adjuvant therapy of tumors
And/or the purposes in medicine.
Additionally, present invention also offers a kind of be used to improve human body immune function and/or prevention and/or adjuvant therapy of tumors
Medicine and/or health food, including the herbal composite and pharmaceutically acceptable auxiliary material.
Present aspect one preferred embodiment in, the formulation of the medicine or health food is capsule, tablet, particle
Agent, oral liquid, powder, tea in bag.
As experiment surprisingly, it has been found that the health food Jing scientific compatibilities obtained by the present invention are formed, bring out the best in each other, nothing
Toxic and side effect.Both it is nutritious, there is the normal immune surveillance function of recovery body again, tumour can be risen and be prevented and/or auxiliary
Help therapeutic action.Experimental verification, the health food of the present invention release the depression effect that MDSC breeds to T cell；Also see after administration
Observe the ratio that complex polysaccharide can lower Treg cells in tumor-bearing mice spleen.Its meaning is that recovery body is normally immune
There is immunologic escape in function for monitoring, prevention of tumor cell, can be used to treat or prevent by immune negative regulation cell MDSC and Treg
Caused tumour and precancerous lesion；Strengthen chemotherapeutic antitumous effect.
The object of the invention provides a kind of with the health food for recovering the normal immune surveillance function of body and its preparation side
Method, while confirming and evaluate wheat-based diet, Inokopolyose, panaxan's combination monitors whether have to recovering body normal immunological
There are prevention and improvement result.Health food prepared by the present invention is commented by recovering the normal immune surveillance function experiment of body
Valency and checking, it should be noted that cited embodiment should not be construed as limiting the invention.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described.
Fig. 1 shows the effect that complex polysaccharide is bred to MSC2；
Fig. 2 gives instructions in reply and closes saccharide portion recovery T cell multiplication capacity；Wherein, Fig. 2 (A) shows control group (splenocyte), Fig. 2 (B)
Show control group+canavaline (ConA), Fig. 2 (C) shows that control group+ConA+MSC2, Fig. 2 (D) show control group+ConA+MSC2+
RCR, Fig. 2 (E) show control group+ConA+MSC2+NX, Fig. 2 (F) show control group+ConA+MSC2+RS, Fig. 2 (G) show control group+
ConA+MSC2+RCR+NX+RS；
Fig. 3 shows that complex polysaccharide lowers colon cancer CT26 tumor-bearing mice MDSC cell proportions；Wherein, Fig. 3 (A) shows control group
Sample, Fig. 3 (B) show the sample of wheat-based diet group, and Fig. 3 (C) shows the sample of Inokopolyose group, and Fig. 3 (D) lets others have a look at gracilis polysaccharide group
Sample, Fig. 3 (E) show the sample of complex polysaccharide group (wheat-based diet+Inokopolyose+panaxan)；
Fig. 4 shows that complex polysaccharide strengthens chemotherapeutic antitumous effect.
Specific embodiment
The invention discloses a kind of polysaccharides of traditional Chinese medicine composition and application thereof, those skilled in the art can use for reference interior herein
Hold, be suitably modified technological parameter realization.Specifically, all similar replacements and change are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.The method of the present invention and application have passed through preferably enforcement
Example is described, related personnel substantially can in without departing from present invention, spirit and scope to method described herein and
Using be modified or suitably change and combine, realize and apply the technology of the present invention.
The invention provides a kind of herbal composite, is made up of saline cistanche, the root of bidentate achyranthes and ginseng.
In some specific embodiments of the present invention, the active ingredient of saline cistanche described in herbal composite is meat desert
Rong's polysaccharide, the active ingredient of the root of bidentate achyranthes is Inokopolyose, and the active ingredient of the ginseng is panaxan.
In some specific embodiments of the present invention, wheat-based diet described in herbal composite, the root of bidentate achyranthes are more
The sugared mass ratio with the panaxan is (1～5):(1～5):(1～5).
In some specific embodiments of the present invention, wheat-based diet described in herbal composite, the root of bidentate achyranthes are more
The sugared mass ratio with the panaxan is (2～4):(2～4):(2～4).
According to a first aspect of the present invention, there is provided a kind of health-care food composition, the composition comprising wheat-based diet,
Inokopolyose, panaxan, preferably by weight 2:3:3 ratios mix, and optionally further include that medical science or food are acceptable
Carrier, excipient, excipient and/or filler.
Present aspect one preferred embodiment in, the wheat-based diet, Inokopolyose or panaxan are natural
Or it is artificial synthesized.
According to a second aspect of the present invention, there is provided a kind of preparation method of health-care food composition, methods described include, mix
By weight 2:3:The wheat-based diet of 3 ratios, Inokopolyose and panaxan.
In one preferred embodiment of the invention, the wheat-based diet, Inokopolyose or panaxan are by as follows
Method is obtained：Take the saline cistanche, the root of bidentate achyranthes or the ginseng to crush, according to saline cistanche, the root of bidentate achyranthes or ginseng: water=0.5g～
2.0g: 5mL ratio adds water, and in terms of g/mL, at 70 DEG C～75 DEG C, heating stirring extracts 3h, filters the filter residue for obtaining according to upper
State solid-liquid ratio, temperature and time to continue to extract twice, merge No. three extracts, clarification concentration is obtained through reduced pressure concentration and centrifugation
Liquid, according to clarification concentrate: 95% ethanol=1: 95% ethanol of corresponding 7～10 times of volumes is added to by 7～10 volume ratio
In clarification concentrate, the overnight stand precipitation at 4 DEG C is mixed, precipitation is collected by centrifugation, meat is obtained through vacuum freeze drying
Desert cistanche polysaccharide, Inokopolyose or panaxan.
According to a third aspect of the present invention, there is provided a kind of health-care food composition prepared according to the method for second aspect.
According to a fourth aspect of the present invention, there is provided a kind of composition and/or Chinese herbal medicine group obtained in described preparation method
Compound is in the preparation for releasing the MDSC inhibitor bred to T cell and/or lowering tumor mice Treg cell proportions is prepared
Using.
Present invention also offers described herbal composite and/or herbal composite obtained in described preparation method
Purposes in the health food and/or medicine for improving immunity function is prepared.
Present invention also offers described herbal composite and/or herbal composite obtained in described preparation method
Purposes in the health food and/or medicine of prevention and/or adjuvant therapy of tumors is prepared.
Present invention also offers described herbal composite and/or herbal composite obtained in the preparation method exist
Prepare the purposes in the medicine for recovering the normal immune surveillance function of body.
It is described to recover the normal immune surveillance function of body, including the depression effect that MDSC breeds to T cell is released, recover
The ability of T cell propagation；Treg cell proportions in tumor mice spleen are lowered, improves immune state；Treatment prevents by immunity
Disease caused by negative regulation cell MDSC and Treg, the disease include tumour and the precancerosis caused by immunosuppressive condition
Become；Strengthen chemotherapeutic antitumous effect.
Present aspect one preferred embodiment in, the formulation of the medicine or health food is capsule, tablet, particle
Agent, oral liquid, powder, tea in bag.
As experiment surprisingly, it has been found that the health food Jing scientific compatibilities obtained by the present invention are formed, bring out the best in each other, nothing
Toxic and side effect.Both it is nutritious, there is the normal immune surveillance function of recovery body again, tumour can be risen and be prevented and/or auxiliary
Help therapeutic action.Experimental verification, the health food of the present invention release the depression effect that MDSC breeds to T cell；Also see after administration
Observe the ratio that complex polysaccharide can lower Treg cells in tumor-bearing mice spleen.Its meaning is that recovery body is normally immune
There is immunologic escape in function for monitoring, prevention of tumor cell, can be used to treat or prevent by immune negative regulation cell MDSC and Treg
Caused tumour and precancerous lesion；Strengthen chemotherapeutic antitumous effect.
The object of the invention provides a kind of with the health food for recovering the normal immune surveillance function of body and its preparation side
Method, at the same confirm and evaluate wheat-based diet, Inokopolyose combination to recover body normal immunological monitor whether with prevention and
Improvement result.Health food prepared by the present invention is evaluated and is tested by recovering the normal immune surveillance function experiment of body
Card, it should be noted that cited embodiment should not be construed as limiting the invention.The Chinese herbal medicine combination that the present invention is provided
Raw materials used and reagent in thing and preparation method thereof, purposes and the medicine containing the herbal composite, health food
Buied by market.
With reference to embodiment, the present invention is expanded on further：
The preparation of 1 wheat-based diet of embodiment
Wheat-based diet of the present invention is prepared by following preparation method：After saline cistanche is crushed, according to meat desert
Rong: the ratio of water=0.5g: 5mL adds water, at 70 DEG C, heating stirring extracts 2h, filters the filter residue for obtaining according to above-mentioned feed liquid
Continue to extract twice than, temperature and time, merge No. three extracts, clarification concentrate is obtained through reduced pressure concentration and centrifugation, pressed
According to clarification concentrate: 95% ethanol=1: 7 volume ratio is added to 7 times of 95% ethanol of volume in clarification concentrate, after mixing
At 4 DEG C, overnight stand precipitation, is collected by centrifugation precipitation, obtains wheat-based diet through vacuum freeze drying.
The preparation of 2 Inokopolyose of embodiment
Inokopolyose of the present invention is prepared by following preparation method：After the root of bidentate achyranthes is crushed, according to the root of bidentate achyranthes: water=
1g: 5mL ratio adds water, at 75 DEG C heating stirring extract 3h, filter obtain filter residue according to above-mentioned solid-liquid ratio, temperature and when
Between continue extract twice, merge No. three extracts, through reduced pressure concentration and centrifugation obtain clarification concentrate, according to clarification concentrate
: 95% ethanol=1: 9 volume ratio is added to 9 times of 95% ethanol of volume in clarification concentrate, mixes overnight quiet at 4 DEG C
Precipitation is put, precipitation is collected by centrifugation, Inokopolyose is obtained through vacuum freeze drying.
The preparation of 3 panaxan of embodiment
Inokopolyose of the present invention, panaxan are prepared by following preparation method：After ginseng is crushed, according to
Ginseng: the ratio of water=2g: 5mL adds water, at 75 DEG C, heating stirring extracts 4h, filters the filter residue for obtaining according to above-mentioned feed liquid
Continue to extract twice than, temperature and time, merge No. three extracts, clarification concentrate is obtained through reduced pressure concentration and centrifugation, pressed
According to clarification concentrate: 95% ethanol=1: 10 volume ratio is added to 10 times of 95% ethanol of volume in clarification concentrate, mixes
At 4 DEG C, overnight stand precipitation, is collected by centrifugation precipitation, obtains panaxan through vacuum freeze drying.
The preparation of 4 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 2 by weight:3:3 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
The preparation of 5 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 1 by weight:5:5 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
The preparation of 6 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 5 by weight:1:1 mixing, further adds medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
The preparation of 7 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 2 by weight:4:3 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
The preparation of 8 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 4 by weight:2:4 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
The preparation of 9 wheat-based diet of embodiment, Inokopolyose and panaxan's composition
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
Ratio 3 by weight:3:2 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.
10 polysaccharide composition of embodiment is to prevention or the checking of adjuvant therapy of tumors function
Present invention applicant is using the wheat-based diet obtained by above-described embodiment 1～4, Inokopolyose, panaxan, multiple
Closing polysaccharide has carried out cytologic experiment, and experimental result is as follows：
1 experiment material
1.1 laboratory apparatus
Flow cytometer：BD companies, Calibo；
Assay balance：Beijing Sai Duolisi Co., Ltds product, Sartorius BS124S types；
Electronic balance：Beijing Sai Duolisi Co., Ltds product, Sartorius BL1500 types.
1.2 experiment reagent
CFSESigma is purchased from company.CD4+, CD8+, CD25+ streaming antibody is purchased from BD companies.
1.3 Experimental agents
1 cell experiment resourcing manifest of table
2 experimental techniques
The effect that 2.1 complex polysaccharides are bred to MDSC
MSC2 is inoculated in 96 orifice plates, and cell density is 1 × 104/ hole.Wheat-based diet, Inokopolyose and panaxan add
Enter in cultivating system.The concentration of single polysaccharide group polysaccharide is respectively 1mg/ml, and complex polysaccharide group total concentration is 1mg/ml, wherein often
It is 2 to plant polysaccharide concentration ratio：3：3.After effect 3 days, MTT detections are carried out.
2.2 mouse junction cancer subcutaneous transplantation knurl model constructions and complex polysaccharide are intervened in vitro
Colon carcinoma cell line CT26, in Balb/c mouse, dosage of inoculation is 1 × 10 for subcutaneous vaccination6/ only, after 21 days, put to death
Mouse, takes spleen and prepares single cell suspension, is inoculated in 96 orifice plates by 4 × 106/ holes, and experimental group is compound to add in cultivating system
Polysaccharide (wheat-based diet+Inokopolyose+panaxan), control group include do not add polysaccharide group and 3 kinds of polysaccharide alone group.Make
With flow cyctometry detection is carried out after 1 day, ratio of the CD11b+Gr1+ cells in monocyte is detected.
2.3 complex polysaccharides are combined with chemotherapeutic
Colon carcinoma cell line CT26, in Balb/c mouse, dosage of inoculation is 1 × 10 for subcutaneous vaccination6/ only.Adopt daily simultaneously
With complex polysaccharide (wheat-based diet+Inokopolyose+panaxan) gavage, once a day.Meanwhile, hypodermic injection 5 FU 5 fluorouracil
(5Fu), dosage is 5 mg kg days.Complex polysaccharide dosage is 500 mg kg days.Control group adopts distilled water gavage,
Daily also hypodermic injection 5Fu, dosage is with complex polysaccharide group.Every group of Sample size is 8.When the 12nd day, start recording tumour is big
Little (long and wide), calculates gross tumor volume, and formula is 1/2 × it is long × wide2.Terminate experiment when 21 days.
3 experimental results
The effect that 3.1 complex polysaccharides are bred to MSC2, is shown in Fig. 1, table 2.
The depression effect that 2 complex polysaccharide of table is bred to MDSC clones (MSC2 cells)
Note：*：Complex polysaccharide is compared with control group, P ＜ 0.01；#：Complex polysaccharide is compared with each single polysaccharide group, P ＜
0.01。
As a result show, complex polysaccharide group MDSC propagation is considerably less than control group, also less than alone group of polysaccharide.The explanation of this result
Complex polysaccharide (wheat-based diet+Inokopolyose+panaxan) can produce Synergistic, suppress MDSC, improve exempting from for body
Epidemic disease state, can be used to prepare health products and food, can be used for prevention and the auxiliary treatment of tumour.
T cell expanding capacity is recovered in 3.2 complex polysaccharide parts, sees Fig. 2, table 3.
The effect of the suppression that 3 complex polysaccharide of table reverse MDSC breeds to T cell
Note：*：Complex polysaccharide is compared with control group, P ＜ 0.01；#：Complex polysaccharide is compared with single polysaccharide group, P ＜ 0.01.
As a result show, complex polysaccharide group T cell propagation obtains part recovery, propagation peak occur, and alone group of polysaccharide does not go out
Now breed peak.This result explanation complex polysaccharide (wheat-based diet+Inokopolyose+panaxan) can be with past release MDSC to T
Cell propagation inhibitory action, play immunoregulation effect, can be used to prepare health products and food, can be used for tumour prevention and
Auxiliary treatment.
3.3 complex polysaccharides lower colon cancer CT26 tumor-bearing mice Treg cell proportions, see Fig. 3, table 4.
4 complex polysaccharide of table lowers the ratio of Treg in CT26 tumor-bearing mice spleens
Note：*：Complex polysaccharide is compared with control group, P ＜ 0.01；#：Complex polysaccharide is compared with single polysaccharide group, P ＜ 0.05.
As a result show, complex polysaccharide group Treg cell proportion is less than control group.(saline cistanche is more for this result explanation complex polysaccharide
Sugar+Inokopolyose+panaxan) tumor mice Treg cell proportions can be lowered, improve the immune state of body, can be used to make
Standby health products and food, can be used for prevention and the auxiliary treatment of tumour.
3.4 complex polysaccharides collaboration chemotherapeutic is antitumor, sees Fig. 4, table 5.
The effect of inhibitor against colon carcinoma cells in 5 complex polysaccharide body of table
Note：*：When the 21st day, complex polysaccharide is compared with control group, P ＜ 0.01.
As a result show, when the 15th day, complex polysaccharide and chemotherapeutic combination group, gross tumor volume are significantly less than control group.This knot
Fruit explanation complex polysaccharide (wheat-based diet+Inokopolyose+panaxan) can strengthen the antitumous effect of chemotherapeutic, can be used for
The auxiliary treatment of chemotherapy of tumors.
Based on the above results, the wheat-based diet of the preparation of embodiment 4, Inokopolyose, panaxan's composition can be released
The depression effect that MDSC breeds to T cell；It was additionally observed that complex polysaccharide can lower the ratio of Treg cells in tumor-bearing mice spleen
Example.Its meaning is to recover the normal immune surveillance function of body, prevention of tumor cell occurs immunologic escape, and prevention of tumor is sent out
It is raw.Thus, show the embodiment of the present invention 4 prepare wheat-based diet, Inokopolyose, panaxan's composition have recover body
Normal immune surveillance function, and than wheat-based diet, Inokopolyose, panaxan more advantage (p is used alone<
0.05)。
The wheat-based diet of the preparation of 5～embodiment of Example 9, Inokopolyose, panaxan's composition carry out above-mentioned reality
Test, the result of the composition that experimental result is prepared with embodiment 4 is same or like, it is more than wheat-based diet, the root of bidentate achyranthes is used alone
Sugar has more advantage, and has significant difference (P≤0.05).
Had according to the wheat-based diet, Inokopolyose, panaxan's composition and recover the normal immunosurveillance work(of body
Can, by natural, artificial, and wheat-based diet, Inokopolyose, people made by the preparation method of above-described embodiment 1,2 and 3
Gracilis polysaccharide, prepares (composition that i.e. prepared by embodiment 4, test discovery 2 in proportion:3: 3 proportional arrangement effects are most unexpected),
Edible or pharmaceutic adjuvant is added, the various health care foods such as capsule, tablet, granule, oral liquid, powder, tea in bag can be prepared into
The formulation of product.
The wheat-based diet of 11 different proportion of embodiment, Inokopolyose and panaxan's composition and its effect
Wheat-based diet, embodiment 2 obtained in embodiment 1 are obtained into Inokopolyose and panaxan obtained in embodiment 3
The ratio mixing in terms of Different Weight in table 6, further add medical science or food acceptable carrier, excipient, excipient and/or
Filler.Make the health-care food composition comprising wheat-based diet, Inokopolyose and panaxan.And according to above-described embodiment
In can to tumour rise prevention and/or auxiliary therapeutic action verification method, the body normal immunological function for monitoring that is restored effect
The result score value of power.(100 points of systems, with embodiment 42:3:3 mixing quality is just recovering body accordingly than prepared composition
Often immune surveillance function effect is designated as 100 points, and the corresponding function test result of other mixed proportions compares the function of accounting for embodiment 4
The percentage point of result of the test, is designated as corresponding function score value)
The wheat-based diet of 6 different proportion of table, Inokopolyose and panaxan's composition and its effect
Function test result finds wheat-based diet, Inokopolyose and panaxan in (1～5):(1～5):(1～5)
Compare during mixed proportion, to tumour
Play prevention and auxiliary therapeutic action.It is (2～4) when polysaccharide mixed proportion is applied:(2～4):When (2～4), effect is more notable.
Health food Jing scientific compatibilities of the present invention are formed, and are brought out the best in each other, are had no toxic side effect；It was both nutritious,
There is the normal immune surveillance function of recovery body again.Applicant states that above content is to this with reference to specific embodiment
Bright made further description, it is impossible to which what identification was of the invention is embodied as being confined to these explanations.For institute of the present invention
For the those of ordinary skill of category technical field, without departing from the inventive concept of the premise, can also make and some simply pushing away
Drill or replace, should all be considered as belonging to protection scope of the present invention.
Claims (10)
1. a kind of herbal composite, it is characterised in that be made up of saline cistanche, the root of bidentate achyranthes and ginseng.
2. herbal composite according to claim 1, it is characterised in that the active ingredient of the saline cistanche is saline cistanche
Polysaccharide, the active ingredient of the root of bidentate achyranthes is Inokopolyose, and the active ingredient of the ginseng is panaxan.
3. herbal composite according to claim 2, it is characterised in that the wheat-based diet, the Inokopolyose
Mass ratio with the panaxan is (1～5):(1～5):(1～5).
4. herbal composite according to claim 2, it is characterised in that the wheat-based diet, the Inokopolyose
Mass ratio with the panaxan is (2～4):(2～4):(2～4).
5. herbal composite according to claim 2, it is characterised in that the wheat-based diet, the Inokopolyose
Mass ratio with the panaxan is 2:3:3.
6. the herbal composite according to any one of claim 1 to 5, it is characterised in that the wheat-based diet, the root of bidentate achyranthes
Polysaccharide or panaxan comprise the following steps：After crushing saline cistanche, the root of bidentate achyranthes or ginseng respectively, in terms of g/mL, according to meat desert
Rong, the root of bidentate achyranthes or people participate in the mass volume ratio of water and mix for the ratio of (0.5g～2.0g): 5mL, heat at 70 DEG C～75 DEG C
2～4h is extracted in stirring, filter residue is collected by filtration and repeats to extract twice according to above-mentioned solid-liquid ratio, temperature and time, merge three extractions
Liquid, obtains clarification concentrate through reduced pressure concentration and centrifugation, is 1 according to the volume ratio of clarification concentrate and 95% ethanol: (7～
10) mix, staticly settle in 4 DEG C, precipitation is collected by centrifugation, wheat-based diet, Inokopolyose are obtained through vacuum freeze drying
Or panaxan.
7. the herbal composite according to any one of claim 1 to 6 is preparing the suppression that releasing MDSC breeds to T cell
Application in the preparation of agent and/or downward tumor mice Treg cell proportions.
8. the herbal composite according to any one of claim 1 to 6 prepare improve immunity function health food and/
Or the purposes in medicine.
9. the herbal composite according to any one of claim 1 to 6 is preparing the guarantor of prevention and/or adjuvant therapy of tumors
Purposes in health food and/or medicine.
10. it is a kind of for improve human body immune function and/or prevention and/or adjuvant therapy of tumors medicine and/or health food,
Characterized in that, including the herbal composite as described in any one of claim 1 to 6 and pharmaceutically acceptable auxiliary material.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580078A (en) * | 2004-01-05 | 2005-02-16 | 吉林威威药业股份有限公司 | Method for preparing ginseng polysaccharide |
| CN101002858A (en) * | 2006-12-19 | 2007-07-25 | 戴甲木 | Medicine composition for enhancing immunity, and its preparing method and use |
| CN104940287A (en) * | 2015-07-20 | 2015-09-30 | 南京中医药大学 | Traditional Chinese medicinal effective ingredient composition and application thereof to preparation of anti-tumor metastasis drugs |
| CN105902597A (en) * | 2016-05-31 | 2016-08-31 | 无限极（中国）有限公司 | Polysaccharide composition, preparation method and use thereof |
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2016
- 2016-10-27 CN CN201610957660.8A patent/CN106511485A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580078A (en) * | 2004-01-05 | 2005-02-16 | 吉林威威药业股份有限公司 | Method for preparing ginseng polysaccharide |
| CN101002858A (en) * | 2006-12-19 | 2007-07-25 | 戴甲木 | Medicine composition for enhancing immunity, and its preparing method and use |
| CN104940287A (en) * | 2015-07-20 | 2015-09-30 | 南京中医药大学 | Traditional Chinese medicinal effective ingredient composition and application thereof to preparation of anti-tumor metastasis drugs |
| CN105902597A (en) * | 2016-05-31 | 2016-08-31 | 无限极（中国）有限公司 | Polysaccharide composition, preparation method and use thereof |
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