CN101380346B - Traditional Chinese composition for treating tumor and production method thereof - Google Patents
Traditional Chinese composition for treating tumor and production method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for treating tumors, the traditional Chinese medicine composition comprises ganoderma lucidum, ganoderma sinensis, coriolus versicolor, antrodia camphorate, agaricus blazei, grifola frondosa, ginseng and ganoderma lucidum spore oil; or the traditional Chinese medicine composition is prepared by taking ganoderma lucidum, ganoderma sinensis, coriolus versicolor, antrodia camphorate, agaricus blazei, grifola frondosa, ginseng and ganoderma lucidum as raw materials and further comprises ganoderma lucidum extract, ganoderma sinensis extract, coriolus versicolor extract, antrodia camphorate extract, agaricus blazei extract, grifola frondosa extract, ginseng extract and the ganoderma lucidum spore oil. The traditional Chinese medicine composition for treating the tumors can assist to suppress the growth of the tumors, resist the injuries of chemo-radiation therapy, promote the bone marrow hematopoiesis and improve the immunity. The invention further relates to a production method of the traditional Chinese medicine composition for treating the tumors.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition of treating tumor, relating in particular to a kind of is that the Mycophyta that raw material is processed is treated the Chinese medicine composition of tumor and the manufacture method of this Chinese medicine composition with Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng, Ganoderma spore oil.
Background technology
Tumor incidence and mortality rate rise year by year, and the serious threat human health has become one of human main causes of death.At present, mostly the inoperable tumor of doctor trained in Western medicine is with chemotherapy and radiation is main, and still, antineoplastic chemotherapy medicine and radiotherapy all have serious side reaction, causes bone marrow hematogenesis obstacle, gastrointestinal reaction and constitutional secondary tumor etc. easily.And Chinese medicine is aspect the treatment tumor, and relatively chemotherapy is less with radiotherapy side effect, and the damage that can cause anti-tumor chemotherapeutic and radiotherapy, the immune indexes of raising tumor patient.Therefore, the Chinese medicine of development adjuvant therapy of tumors, drug combination becomes trend in oncotherapy.
In recent years, quite active about the research of fungus antitumor, a lot of funguses are applied in the middle of the clinical treatment, play the effect of the auxiliary chemotherapy that suppresses tumor and protection tumour patient, damage that radiotherapy causes.Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa all are the wider antitumor funguses of domestic and international application.Radix Ginseng then is China's tradition rare Chinese medicine, and its pharmacological component ginsenoside activity is various, especially has splendid anti-tumor activity.
Therefore; Being necessary to provide a kind of is that the Mycophyta that raw material is processed is treated the Chinese medicine composition of tumor and the manufacture method of this Chinese medicine composition with Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng, Ganoderma spore oil, effectively resists chemoradiotherapy damage, auxiliary tumor, promotion bone marrow hematogenesis, the raising immunity of suppressing to use this Chinese medicine composition.
Summary of the invention
The objective of the invention is; The Chinese medicine that lacks the damage of antagonism chemoradiotherapy to present treatment tumor; Select rare Chinese medicine; Provide a kind of and can assist the Chinese medicine composition that suppresses tumor, the damage of antagonism chemoradiotherapy, promotes the treatment tumor of bone marrow hematogenesis, raising immunity, be applied in the oncotherapy field, particularly the application in the medicine of preparation treatment tumor or bone marrow hematogenesis.
Another object of the present invention is that the selection rare Chinese medicine through screening and optimizing prescription, appropriate design technological process, provides a kind of making can assist the method for the Chinese medicine composition of inhibition tumor, the damage of antagonism chemoradiotherapy, promotion bone marrow hematogenesis, raising immunity.
Below will further specify the technical scheme that realizes the foregoing invention purpose.
A kind of Chinese medicine composition of treating tumor; It comprises Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma spore oil, and perhaps it is that raw material is processed and comprised Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract, Radix Ginseng extract, Ganoderma spore oil by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma.
Charge ratio (weight) in the Chinese medicine composition of the said treatment tumor that comprises Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma spore oil is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. 1-90%, Ganoderma 1-90%, Coriolous Dersicolor (Fr.) Quel 1-90%, Antrodia camphorata 1-90%, Agaricus blazei Murrill 1-90%, Grifola frondosa 1-90%, Radix Ginseng 1-90% and Ganoderma spore oil 1-90%.Wherein, Antrodia camphorata can be selected sporophore or mycelium for use, and Agaricus blazei Murrill can be selected sporophore or mycelium for use, and Grifola frondosa can be selected sporophore or mycelium for use.
Preferred formulations in the Chinese medicine composition of the said treatment tumor that comprises Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma spore oil than (weight) is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. 1-20%, Ganoderma 1-20%, Coriolous Dersicolor (Fr.) Quel 1-20%, Antrodia camphorata 1-20%, Agaricus blazei Murrill 1-20%, Grifola frondosa 1-20%, Radix Ginseng 1-20% and Ganoderma spore oil 30-70%.
Said is that the weight proportion of each component in the Chinese medicine composition of the treatment tumor processed of raw material is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 1-90%, Ganoderma extract 1-90%, Coriolus Versicolor P.E. 1-90%, Antrodia camphorata extract 1-90%, Agaricus blazei Murrill extract 1-90%, Grifola frondosa extract 1-90%, Radix Ginseng extract 1-90%, Ganoderma spore oil 1-90% by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma.Wherein, Antrodia camphorata can be selected sporophore or mycelium for use, and Agaricus blazei Murrill can be selected sporophore or mycelium for use, and Grifola frondosa can be selected sporophore or mycelium for use.
Said is that the preferred weight proportioning of each component in the Chinese medicine composition of the treatment tumor processed of raw material is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 1-20%, Ganoderma extract 1-20%, Coriolus Versicolor P.E. 1-20%, Antrodia camphorata extract 1-20%, Agaricus blazei Murrill extract 1-20%, Grifola frondosa extract 1-20%, Radix Ginseng extract 1-20%, Ganoderma spore oil 30-70% by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma.
A kind of manufacture method of treating the Chinese medicine composition of tumor, it may further comprise the steps:
The first step prepares each component of the Chinese medicine composition of said treatment tumor respectively: Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract.
Above-mentioned each component can take the water concentration to contract or the water extract-alcohol precipitation mode prepares; Promptly get Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore, Ganoderma spore, Coriolous Dersicolor (Fr.) Quel spore, the dry sporophore of Antrodia camphorata or mycelium, the dry sporophore of Agaricus blazei Murrill or mycelium, the dry sporophore of Grifola frondosa or mycelium, dry Radix Ginseng respectively; Carry out micronizing to obtain the powder of above-mentioned each raw material respectively; Adopt supersonic wave wall breaking again, with the breaking cellular wall powder of the powder that obtains above-mentioned each raw material respectively; The breaking cellular wall powder of the powder of above-mentioned each raw material of reflux extraction, and, extract the water-soluble components in each raw material with the ethanol precipitation of 5-95%.Preferably, carry out micronizing before, direct above-mentioned each raw material of lyophilization.Adopt the concrete manufacturing process of water extract-alcohol precipitation mode following:
(1) breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder, preparation Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract
Get the Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets the Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder; Perhaps direct lyophilization Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore is processed the Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder the Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder.
Get breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract the Ganoderma lucidum (Leyss. Ex Fr.) Karst. water-soluble components, mainly be that the polysaccharide body comprises polysaccharide, glycopeptide, glycoprotein, thereby obtain the Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract.
(2) breaking cellular wall Ganoderma spore powder, preparation Ganoderma extract
Get the Ganoderma spore, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets the Ganoderma spore powder; Perhaps direct lyophilization Ganoderma spore is processed the Ganoderma spore powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Ganoderma spore powder the Ganoderma spore powder.
Get breaking cellular wall Ganoderma spore powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract the Ganoderma water-soluble components, mainly be that the polysaccharide body comprises polysaccharide, glycopeptide, glycoprotein, thereby obtain the Ganoderma extract.
(3) breaking cellular wall Coriolous Dersicolor (Fr.) Quel spore powder, the preparation Coriolus Versicolor P.E.
Get the Coriolous Dersicolor (Fr.) Quel spore, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets the Coriolous Dersicolor (Fr.) Quel spore powder; Perhaps direct lyophilization Coriolous Dersicolor (Fr.) Quel spore is processed the Coriolous Dersicolor (Fr.) Quel spore powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Coriolous Dersicolor (Fr.) Quel spore powder the Coriolous Dersicolor (Fr.) Quel spore powder.
Get breaking cellular wall Coriolous Dersicolor (Fr.) Quel spore powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract the Coriolous Dersicolor (Fr.) Quel water-soluble components, mainly be that the polysaccharide body comprises polysaccharide, glycopeptide, glycoprotein, thereby obtain Coriolus Versicolor P.E..
(4) breaking cellular wall Antrodia camphorata sporophore or mycelium powder, preparation Antrodia camphorata extract
Get dry sporophore of Antrodia camphorata or mycelium, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets Antrodia camphorata sporophore or mycelium powder; Perhaps direct lyophilization Antrodia camphorata sporophore or mycelium are processed Antrodia camphorata sporophore or mycelium powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Antrodia camphorata sporophore or mycelium powder Antrodia camphorata sporophore or mycelium powder.
Get breaking cellular wall Antrodia camphorata sporophore or mycelium powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract Antrodia camphorata polysaccharide body, triterpenes components, obtain the Antrodia camphorata extract.
(5) breaking cellular wall Agaricus blazei Murrill sporophore or mycelium powder, preparation Agaricus blazei Murrill extract
Get dry sporophore of Agaricus blazei Murrill or mycelium, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets Agaricus blazei Murrill sporophore or mycelium powder; Perhaps direct lyophilization Agaricus blazei Murrill sporophore or mycelium are processed Agaricus blazei Murrill sporophore or mycelium powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Agaricus blazei Murrill sporophore or mycelium powder Agaricus blazei Murrill sporophore or mycelium powder.
Get breaking cellular wall Agaricus blazei Murrill sporophore or mycelium powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract the Agaricus Blazei Murrill polysaccharide body, obtain the Agaricus blazei Murrill extract.
(6) breaking cellular wall Grifola Frondosa sporophore or mycelium powder, preparation Grifola frondosa extract
Get dry sporophore of Grifola frondosa or mycelium, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets Grifola Frondosa sporophore or mycelium powder; Perhaps direct lyophilization Grifola Frondosa sporophore or mycelium are processed Grifola Frondosa sporophore or mycelium powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Grifola Frondosa sporophore or mycelium powder Grifola Frondosa sporophore or mycelium powder.
Get breaking cellular wall Grifola Frondosa sporophore or mycelium powder, with 5-10 times of water gaging reflux extraction 1-3 time, each 0.5-3 hour, with the ethanol precipitation of 5-95%, extract the grifolan body, obtain the Grifola frondosa extract.
(7) breaking cellular wall Radix Ginseng dry powder, the preparation Radix Ginseng extract
Get dry Radix Ginseng, carry out micronizing, the granularity of pulverizing is the 80-300 order, gets Radix Ginseng dry powder; Perhaps the lyophilization Radix Ginseng is processed Radix Ginseng dry powder through micronizing.Adopt supersonic wave wall breaking to obtain breaking cellular wall Radix Ginseng dry powder in Radix Ginseng dry powder.
Get breaking cellular wall Radix Ginseng dry powder,, each 0.5-3 hour,, extract ginseng polysaccharide's body, Saponin constituents, thereby obtain Radix Ginseng extract with the ethanol precipitation of 5-95% with 5-10 times of water gaging reflux extraction 1-3 time.
Second step, the preparation Ganoderma spore oil.
Said Ganoderma spore oil can take supercritical carbon dioxide extraction, squeezing to extract or Suo Shi extracts preparation.When adopting supercritical carbon dioxide extraction to extract preparation; Get dry Ganoderma spore powder, carry out supercritical carbon dioxide extraction, extraction conditions is 25-35MPa; Extraction temperature is 40-45 ℃; Extraction 2-3 hour obtains Ganoderma spore oil, and its main component is fatty acid, Ganoderma triterpenoids constituents.
The 3rd step, get Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract, be base oil with the Ganoderma spore oil, add pharmaceutic adjuvant and mix, process medicament.Preferably, used pharmaceutic adjuvant is Cera Flava, hydrogenated oil and fat, gelatin, glycerol, water, processes soft capsule by conventional method.
With respect to prior art, the Chinese medicine composition of the treatment tumor of present technique scheme is processed soft capsule, shows the effect with adjuvant therapy of tumors after deliberation.Result of the test shows: (1) is to transplanted sarcoma S
180Mouse stomach gives this Chinese medicine composition, can significantly suppress mice transplanted sarcoma S
180The propagation of tumor cell explains that these article have stronger anti-tumor activity; (2) tumor-bearing mice is irritated stomach and give this Chinese medicine composition, can increase the quantity of mice with tumor thymus and splenocyte, explain that this Chinese medicine composition can significantly improve immune ability; (3) the raying injured mice is irritated stomach and give this Chinese medicine composition, can alleviate bone marrow depression, recover hemopoietic function of bone marrow.(4) irritate stomach and give the rabbit mortality rate that this Chinese medicine composition can reduce the tetrachloro-methane induction hepatic injury, alleviate injured degree of hepatic tissue and the destruction that prevents the hepatocyte device.
Test method:
1, the Chinese medicine composition of this treatment tumor is to transplanted sarcoma S
180The influence of mice and immune organ thereof
Get Kunming kind pure lines healthy mice, 6 ages in week, heavy (mg) body weight 18～22g of the average tumor of test group.Extract abdominal cavity inoculation S
180Cell 7～10d (my god) mouse ascites, be diluted to 1 * 10 with physiological saline solution
7The oncocyte suspension of/ml concentration, inoculation 0.2ml suspension is subcutaneous in the right ventral groove of healthy mice, random packet; Every group 10; Male and female half and half, begin next day with 0.167,0.330, the dosage gastric infusion of 1.000g/dkg once, continuous 10 days; Negative control group (Ck) gives the equivalent physiological saline solution every day, and positive controls (Cy) gives the cyclophosphamide with the physiological saline solution dilution.Animal is put to death in the cervical vertebra dislocation behind the drug withdrawal 24h, peels off the tumor piece, weighs, and is calculated as follows tumor control rate:
Tumor control rate (%)=(1-W
1/ W
2) * 100%
W wherein
1The average tumor of negative matched group heavy (mg), W
2For the average tumor of each test group heavy (mg), take by weighing spleen and thymus simultaneously, weigh, relatively to the influence of immune organ.
Index and spleen index=spleen weight/body weight (mg/g)
Thymus index=thymus weight/body weight (mg/g)
Table 1 should be treated the antitumous effect of the Chinese medicine composition of tumor to mice S180 transplanted tumor
Table 2 should be treated the influence of the Chinese medicine composition of tumor to the mice with tumor immune organ
2, the Chinese medicine composition of this treatment tumor is to the influence of mouse bone marrow cells hemopoietic function
(1) to the influence of mice with radiation injury PBL sum and bone marrow GM-CFU (bone marrow grain macrophage colony forms unit)
Have male and female concurrently BALB/c mouse and divide 3 groups at random, 10 every group, test and surveyed the PBL sum in preceding 1 day, test the same day, the experimental mice warp
60Co gamma-rays 300cGy total irradiation, close rate does<10cGymin, begin with 0.167,0.330 after the irradiation immediately, the dosage gastric infusion of 1.000g/dkg once, continuous 5 days, normal control group (not shining) and irradiation control group (exposure dose is the same) were all injected the equivalent normal saline.Total white blood cells was looked into by tail vein blood respectively on the 1st, 3,5 day in irradiation back, got homonymy femur bone marrow cell, In vitro culture GM-CFU on the 6th day.
(2) to the active influence of mice with radiation injury serum Hemopoietic factor
The donor BALB/c mouse is divided into 3 groups at random, 21 every group, equal warp
60Co gamma-rays 300cGy total irradiation, close rate does<10cGymin, prevention group pre-irradiation, after the treatment group irradiation, begin with 0.167,0.330 immediately, the dosage gastric infusion of 1.000g/dkg once, continuous 5 days, all inject the equivalent normal saline behind the irradiation control group pre-irradiation.Shine and killed mice on the the 6th, 8,10 day in batches, heart puncturing extracting blood is collected in the sterilization centrifuge tube respectively; After leaving standstill 12h; Collect serum as colony stimulating factor, (rat lung strip spare culture fluid reduces by half, adds test serum 0.1ml/ml) cultivated 5 days in the adding GM-CFU cultivating system, detected the GM-CFU productive rate; It is active to observe the serum Hemopoietic factor, detects its activity level after the 6th, 8, the 10 day simultaneously serum mixed in equal amounts.
(3) result
Through 300cGy
60The mice peripheral white blood cell of Co radiation gamma obviously reduces (P<0.01), the Chinese medicine composition gastric infusion leukocyte count bottom out in 3 days through this treatment tumor rose to 6.06 * 10 in 5 days
9/ L, irradiation control group leukocyte count are then by postradiation 3.20 * 10
9/ L continues to drop to 2.35 * 10
9/ L.Point out the Chinese medicine composition of this treatment tumor to have the effect that promotes that the mice with radiation injury PBL recovers.Can promote simultaneously the propagation of mice with radiation injury GM-CFU.
It is active that these article can improve mice with radiation injury serum Hemopoietic factor.Dynamic observe and show: irradiation back the 6th day, prevention group and treatment group serum Hemopoietic factor activity level significantly improve (P < 0.01), then descend gradually after the drug withdrawal, and the 10th day, near matched group level (P>0.05).
3, the Chinese medicine composition of this treatment tumor is to the protective effect of tetrachloro-methane induction hepatic injury rabbit
To test at random that rabbit is divided into I group (liver injury model group), II organizes (administration group), 7 every group.Under same condition, give feedstuff and drinking-water according to normal method.Tested the 1st day; Each organizes the carbon tetrachloride paraffin solution (dosage is 0.4mL/kgBw (ml/kg body weight)) of equal subcutaneous injection 50%, and the I group is irritated stomach and given normal saline simultaneously, and the II group is irritated the Chinese medicine composition that stomach gives this treatment tumor; Dosage is 330mg/kgBw), injected continuously 7 days.After contamination, every day, the observed and recorded clinical symptoms was in time cutd open inspection to dead rabbit, inspection and record pathological change.Tested the 12nd day, (handling the same) all slaughtered, dissected to the survival test rabbit.
Result of the test: I, II group rabbit are after contamination, and I group part rabbit performance spirit is depressed, drowsiness, appetite reduces or useless exhausted, diarrhoea, and reaction reduces to external world.Behind counteracting toxic substances, began in 3 days to occur dead, to the 5th day dead 4, the 11st day dead 1, dead 7 altogether.No abnormality seen then in the II group rabbit 5 days, only behind counteracting toxic substances the 12nd day dead 1.
The specific embodiment
To further specify the present technique scheme through a plurality of embodiment below.
Embodiment 1
A kind of is the method that raw material is made the Chinese medicine composition of treatment tumor by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma, and it may further comprise the steps:
(1) preparation breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder, Ganoderma spore powder, Coriolous Dersicolor (Fr.) Quel spore powder
Get Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore, Ganoderma spore, each 10kg of Coriolous Dersicolor (Fr.) Quel spore respectively, utilize super micron mill, grinding particle size is 300 orders, gets breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powder, Ganoderma spore powder, Coriolous Dersicolor (Fr.) Quel spore powder respectively through supersonic wave wall breaking again.
(2) preparation breaking cellular wall Antrodia camphorata, Agaricus blazei Murrill, ash tree pollen
Get Antrodia camphorata sporophore or mycelium, Agaricus blazei Murrill sporophore or each 10kg of mycelium, Grifola Frondosa sporophore or mycelium respectively; Utilize super micron mill; Grinding particle size is 300 orders, gets breaking cellular wall Antrodia camphorata sporophore or mycelium powder, Agaricus blazei Murrill sporophore or mycelium powder, Grifola Frondosa sporophore or mycelium powder respectively through supersonic wave wall breaking again.
(3) preparation breaking cellular wall Radix Ginseng powder
Get Radix Ginseng dry product 10kg, utilize super micron mill, grinding particle size is 300 orders, gets the breaking cellular wall Radix Ginseng powder through supersonic wave wall breaking again.
(4) preparation Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract
In the present embodiment, adopt the water extract-alcohol precipitation mode to prepare the extract of each raw material.Particularly; Get 300 purpose breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore powders, Ganoderma spore powder, Coriolous Dersicolor (Fr.) Quel spore powder and Antrodia camphorata sporophore or mycelium powder, Agaricus blazei Murrill sporophore or mycelium powder, Grifola Frondosa sporophore or mycelium powder, Radix Ginseng powder 10kg respectively; Use the water heating and refluxing extraction 3 times of 10 times of amounts respectively; Each 2 hours; Ethanol precipitation with 75% obtains Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 3.5kg, Ganoderma extract 3.6kg, Coriolus Versicolor P.E. 4.1kg, Antrodia camphorata extract 3.2kg, Agaricus blazei Murrill extract 3.8kg, Grifola frondosa extract 4.5kg, Radix Ginseng extract 1.3kg respectively.That is to say; 1kg Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract is equivalent to the 2.9kg Ganoderma lucidum (Leyss. Ex Fr.) Karst.; 1kg Ganoderma extract is equivalent to the 2.8kg Ganoderma, and the 1kg Coriolus Versicolor P.E. is equivalent to 2.4kg Coriolous Dersicolor (Fr.) Quel, and 1kg Antrodia camphorata extract is equivalent to the 3.1kg Antrodia camphorata; 1kg Agaricus blazei Murrill extract is equivalent to the 2.6kg Agaricus blazei Murrill, and 1kg Grifola frondosa extract is equivalent to the 2.2kg Grifola frondosa.
(5) preparation Ganoderma spore oil
In the present embodiment, adopt the supercritical carbon dioxide extraction method to prepare Ganoderma spore oil.Particularly, take by weighing Ganoderma spore powder with cellular wall broken 10kg, granulate with 30% ethanol water, throw in extraction kettle, extraction conditions is: temperature is 45 ℃, and pressure is 30MPa, and the time is 2h, and flow is 35kg/h.The secondary separation processes is: first order separating pressure is 10MPa, and separation temperature is 50 ℃, second level separating pressure 6MPa, 40 ℃ of separation temperatures.Collect the Ganoderma spore oil 2.3kg that separation reactor I parses.
(6) Chinese medicine composition of preparation treatment tumor
In the present embodiment; The Chinese medicine composition of the treatment tumor of preparation soft capsule; Particularly Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract, each 2kg of Radix Ginseng extract are mixed,, add adjuvant Cera Flava 0.2kg with 21kg Ganoderma spore oil mixing; Be pressed into soft capsule according to soft capsule technology again, every intragranular is tolerant to be 500mg.
Be appreciated that and also can adopt other pharmaceutical methods commonly used, the mixture of said each extract is processed the medicament of other form, for example tablet, oral administration solution or the like.
Embodiment 2
A kind of is the method for the Chinese medicine composition of the raw material treatment tumor of making by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma, wherein prepares breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel spore powder; Preparation breaking cellular wall Antrodia camphorata, Agaricus blazei Murrill, ash tree pollen; Preparation breaking cellular wall Radix Ginseng powder and preparation Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract; And the step of preparation Ganoderma spore oil is basic identical with embodiment 1.Difference is; The grinding particle size of each raw material is 200 orders; And add 5 times of water yield reflux extractions 3 times; Each 1 hour, in reflux extraction with 95% ethanol precipitation, thereby obtain Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Grifola frondosa extract, Radix Ginseng extract respectively.
In the step of the Chinese medicine composition of this treatment tumor of this external preparation; The Chinese medicine composition that present embodiment will be treated tumor is prepared into soft capsule; Particularly Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 2kg, Ganoderma extract 1kg, Coriolus Versicolor P.E. 1kg, Antrodia camphorata extract 1kg, Agaricus blazei Murrill extract 2kg, Grifola frondosa extract 1kg, Radix Ginseng extract 2kg are mixed; With 10kg Ganoderma spore oil mixing, add adjuvant Cera Flava 0.1kg, hydrogenated oil and fat 0.02kg, gelatin 0.01kg, glycerol 0.01kg; Be pressed into soft capsule according to soft capsule technology again, every intragranular is tolerant to be 500mg.
Be appreciated that and also can adopt other pharmaceutical methods commonly used, said mixture is processed the medicament of other form, for example tablet, oral administration solution or the like.
Embodiment 3
A kind of is the method for the Chinese medicine composition of the raw material treatment tumor of making by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma, wherein prepares breaking cellular wall Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel spore powder; Preparation breaking cellular wall Antrodia camphorata, Agaricus blazei Murrill, ash tree pollen; Preparation breaking cellular wall Radix Ginseng powder and preparation Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract; And the step of preparation Ganoderma spore oil is basic identical with embodiment 1.Difference is; The grinding particle size of each raw material is 80 orders; And add 8 times of water yield reflux extractions 2 times; Each 3 hours, in reflux extraction with 45% ethanol precipitation, thereby obtain Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Grifola frondosa extract, Radix Ginseng extract respectively.
In the step of this external preparation Chinese medicine composition; In the present embodiment; The Chinese medicine composition of preparation soft capsule mixes Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 3kg, Ganoderma extract 3kg, Coriolus Versicolor P.E. 4kg, Antrodia camphorata extract 2kg, Agaricus blazei Murrill extract 2kg, Grifola frondosa extract 2kg, Radix Ginseng extract 4kg, with 5kg Ganoderma spore oil mixing particularly; Add adjuvant Cera Flava 0.1kg; Hydrogenated oil and fat 0.02kg, gelatin 0.01kg, glycerol 0.01kg are pressed into soft capsule according to soft capsule technology again, and every intragranular is tolerant to be 500mg.
Be appreciated that equally and also can adopt other pharmaceutical methods commonly used, said mixture is processed the medicament of other form, for example tablet, oral administration solution or the like.
Embodiment 4
A kind of Chinese medicine composition of treating tumor; It selects Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa and the Radix Ginseng mixing Ganoderma spore oil of corresponding weight to carry out direct pharmacy according to effective ingredient required in Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa and the Radix Ginseng.Be that 1kg Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract is equivalent to the 2.9kg Ganoderma lucidum (Leyss. Ex Fr.) Karst.; 1kg Ganoderma extract is equivalent to the 2.8kg Ganoderma; The 1kg Coriolus Versicolor P.E. is equivalent to 2.4kg Coriolous Dersicolor (Fr.) Quel; 1kg Antrodia camphorata extract is equivalent to the 3.1kg Antrodia camphorata, and 1kg Agaricus blazei Murrill extract is equivalent to the 2.6kg Agaricus blazei Murrill, and 1kg Grifola frondosa extract is equivalent to the 2.2kg Grifola frondosa.The concrete manufacture method of the Chinese medicine composition of said treatment tumor is following: get the 5.8kg Ganoderma lucidum (Leyss. Ex Fr.) Karst. respectively, 5.6kg Ganoderma, 4.8kg Coriolous Dersicolor (Fr.) Quel; 6.2kg Antrodia camphorata, the 5.2kg Agaricus blazei Murrill, the 4.4kg Grifola frondosa is carried out the Ultrasonic Pulverization powdered; Grinding particle size is 300 orders, with 20kg Ganoderma spore oil mixing.It is understandable that, can adopt multiple pharmaceutical methods commonly used, said mixture is processed various forms of medicaments, for example soft capsule, tablet, oral administration solution or the like.
Claims (4)
1. Chinese medicine composition of treating sarcoma; It is that crude drug is processed and comprised Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract, Radix Ginseng extract and Ganoderma spore oil by Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma, Coriolous Dersicolor (Fr.) Quel, Antrodia camphorata, Agaricus blazei Murrill, Grifola frondosa, Radix Ginseng and Ganoderma, and said Chinese medicine composition is prepared from through following steps:
Prepare Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract respectively;
The preparation Ganoderma spore oil, said Ganoderma spore oil is the oiliness concentrate of triterpenes components; And
Getting Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract, add pharmaceutic adjuvant and mix, is base oil with the Ganoderma spore oil, processes medicament, wherein,
Adopt the water extract-alcohol precipitation mode to prepare Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract and Radix Ginseng extract respectively: to get Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore, Ganoderma spore, Coriolous Dersicolor (Fr.) Quel spore, the dry sporophore of Antrodia camphorata or mycelium, the dry sporophore of Agaricus blazei Murrill or mycelium, the dry sporophore of Grifola frondosa or mycelium, dry Radix Ginseng respectively; Carry out micronizing; Obtaining above-mentioned each raw material granularity is 80-300 purpose powder; Adopt supersonic wave wall breaking, obtain the breaking cellular wall powder of the powder of above-mentioned each raw material; Adopt 5-10 times of water gaging reflux extraction 1-3 time, 0.5-3 hour at every turn, and, extract the water-soluble components in each raw material with the ethanol precipitation of 5-95%;
Utilize supercritical carbon dioxide extraction to prepare Ganoderma spore oil: get dry Ganoderma spore powder, carry out supercritical carbon dioxide extraction, extraction conditions is 25-35MPa, and extraction temperature is 40-45 ℃, extraction 2-3 hour.
2. the Chinese medicine composition of treatment sarcoma as claimed in claim 1 is characterized in that: the weight proportion of said Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract, Radix Ginseng extract, Ganoderma spore oil is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 1-90%, Ganoderma extract 1-90%, Coriolus Versicolor P.E. 1-90%, Antrodia camphorata extract 1-90%, Agaricus blazei Murrill extract 1-90%, Grifola frondosa extract 1-90%, Radix Ginseng extract 1-90%, Ganoderma spore oil 1-90%.
3. the Chinese medicine composition of treatment sarcoma as claimed in claim 2 is characterized in that: the weight proportion of said Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract, Ganoderma extract, Coriolus Versicolor P.E., Antrodia camphorata extract, Agaricus blazei Murrill extract, Grifola frondosa extract, Radix Ginseng extract, Ganoderma spore oil is: Ganoderma lucidum (Leyss. Ex Fr.) Karst. extract 1-20%, Ganoderma extract 1-20%, Coriolus Versicolor P.E. 1-20%, Antrodia camphorata extract 1-20%, Agaricus blazei Murrill extract 1-20%, Grifola frondosa extract 1-20%, Radix Ginseng extract 1-20%, Ganoderma spore oil 30-70%.
4. the application of the Chinese medicine composition of treatment sarcoma as claimed in claim 3 in preparation medicine for treating tumor thing, it is characterized in that: said tumor is a sarcoma.
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| JP6093238B2 (en) * | 2013-05-13 | 2017-03-08 | 株式会社リンクス | Hematopoietic progenitor cell recovery agent for radiation damage |
| CN103300368B (en) * | 2013-06-18 | 2016-12-07 | 陕西大志药业有限公司 | The health food of a kind of enhancing immunity and capsule preparation method thereof thereof |
| CN104435756A (en) * | 2014-11-21 | 2015-03-25 | 柳州市天姿园艺有限公司 | Pharmaceutical composition capsule containing antrodia camphorata, and preparation method therefor |
| CN104856238A (en) * | 2015-05-15 | 2015-08-26 | 深圳市杰仕博科技有限公司 | Indirect heating type atomizer |
| CN105920136A (en) * | 2016-04-25 | 2016-09-07 | 哈药集团中药二厂 | Preparation method of pyropolyporus fomentarius teng anticancer tablet |
| CN107485627A (en) * | 2017-08-02 | 2017-12-19 | 袁青贤 | A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour |
| CN110183522B (en) * | 2019-05-29 | 2020-09-18 | 北京市农林科学院 | Glycopeptide with antidepressant function and preparation method and application thereof |
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