CN106508827B - A kind of method and application of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra - Google Patents
A kind of method and application of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra Download PDFInfo
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- CN106508827B CN106508827B CN201610979869.4A CN201610979869A CN106508827B CN 106508827 B CN106508827 B CN 106508827B CN 201610979869 A CN201610979869 A CN 201610979869A CN 106508827 B CN106508827 B CN 106508827B
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 85
- 230000005058 diapause Effects 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 24
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 10
- 230000012447 hatching Effects 0.000 claims abstract description 8
- 238000012545 processing Methods 0.000 claims abstract description 6
- 241000257303 Hymenoptera Species 0.000 claims abstract description 5
- 208000012641 Pigmentation disease Diseases 0.000 claims abstract description 5
- 230000019612 pigmentation Effects 0.000 claims abstract description 5
- 230000008117 seed development Effects 0.000 claims abstract description 4
- 230000032696 parturition Effects 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 abstract description 6
- 235000013601 eggs Nutrition 0.000 description 8
- 235000008708 Morus alba Nutrition 0.000 description 5
- 240000000249 Morus alba Species 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000004321 preservation Methods 0.000 description 3
- 101800004437 Diapause hormone Proteins 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Materials For Medical Uses (AREA)
- Peptides Or Proteins (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
Abstract
The present invention discloses a kind of method and application of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra.The present invention will give birth to after without diapause polyvoltinism silkworm germ plasm resource silkworm seed in 25~26 DEG C, relative humidity be 75%~80% in the environment of protect 38~42 hours；14~16 DEG C of environment is next moved to place 1.5~2.5 hours；Then 1~10 DEG C of environment is moved to stored refrigerated 25~28 days；14~16 DEG C of environment is then moved to place to the silkworm seed development of embryo to body pigmentation stage；It takes out, carries out hiding black processing under 27~28 DEG C of temperature, 80%~85% environment of humidity, promote silkworm seed embryo of silkworms to be smoothly developed to hatching, normally collect ants and raise.The time that the present invention can save no diapause polyvoltinism cold storage of silkworm egg extends 20~22 days.To reduce to for 4 generations by annual 7~8 generation of conventional method to the child care algebra of no diapause polyvoltinism silkworm germ plasm resource, raising number and labor intensity are greatly reduced.
Description
Technical field
The invention belongs to silkworm Germ-plasma resources protection field, in particular to a kind of reduce provides without diapause polyvoltinism silkworm germplasm
The method and application of source child care algebra.
Background technique
By etesian generation number, silkworm germ plasm resource can be divided into univoltine, bivoltine and polyvoltinism, wherein polyvoltine
Property silkworm germplasm is divided into again has duration of diapause polyvoltinism and without two class of duration of diapause polyvoltinism.
No diapause polyvoltinism silkworm germ plasm resource is the Germplasm resources of nature preciousness, is the existing silkworm germplasm in China
The important component of resource and the original material of silkworm genetic breeding and basic scientific research.The in store heat in Guangdong at present
Without more than 40 part of diapause polyvoltinism silkworm germ plasm resource, domestic other teaching and scientific research units are also in store in varying numbers in band subtropical zone
Without diapause polyvoltinism silkworm germplasm.Due to the annual not Diapause of such silkworm germplasm, length can only be passed through by saving to it
Year constantly propagating and breeding, generally need to raise 7-8 generation every year, a large amount of manpower and material resources need to be expended, so that being engaged in this work
Personnel not can guarantee long-term vacation, during the Spring Festival must according to silkworm rearing season carry out work and life arrangement.Every year
Raising of the dormant period of winter mulberry tree also to polyvoltinism silkworm germplasm during this brings certain difficulty, due to certain kinds of confrontation people
Work feed ration size is poor, by man-made feeds carry out the teletostage feeding again will necessarily be brought to the conservation of polyvoltinism silkworm germplasm compared with
Big risk.
In order to reduce every year to the child care algebra of no diapause polyvoltinism silkworm germ plasm resource, researcher is to this progress
More exploration and trial.In the report of forefathers, solution for this problem mainly makes no diapause polyvoltinism silkworm egg
Diapause silkworm seed is generated, including uses control hatching period of silkworm egg temperature and humidity and sericulture phase light application time to obtain Diapausing egg, is infused to silkworm chrysalis
Micro diapause hormone is penetrated to obtain the methods of Diapausing egg, restores it after second year termination of diapause again without diapause polyvoltinism.But due to
The Diapause rate for controlling temperature and humidity and the produced silkworm seed of illumination condition is unstable, and the operation processing for injecting diapause hormone has relatively strictly
Technical requirements.
In view of the above-mentioned problems, research is not under conditions of influencing main morphological features and economic characters to no diapause polyvoltinism
The new method that the silkworm egg of silkworm germplasm is protected provides with reference to approach more effectively to save such resource, meets Labor-saving
Efficient silkworm modern times yard feeding pattern is protected to reducing without the annual child care algebra of diapause polyvoltinism silkworm germ plasm resource, reduction
It plants labor intensity and conservation cost has important practical significance and broad application prospect.
Summary of the invention
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and deficiency, provide a kind of reduce without diapause polyvoltinism
The method of silkworm germ plasm resource child care algebra.
Another object of the present invention is to provide the side of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra
The application of method.
The purpose of the invention is achieved by the following technical solution: a kind of to reduce without diapause polyvoltinism silkworm germ plasm resource child care
The method of algebra, includes the following steps:
(1) without diapause polyvoltinism silkworm germ plasm resource silkworm seed after giving birth in temperature is 25~26 DEG C, relative humidity is
It is protected 38~42 hours in the environment of 75%~80%；
(2) 14~16 DEG C of environment is next moved to place 1.5~2.5 hours；
(3) environment stored refrigerated 25~28 days of 1~10 DEG C are then moved to；
(4) 14~16 DEG C of environment is then moved to place to the silkworm seed development of embryo to body pigmentation stage；
(5) it takes out, carries out hiding black processing under 27~28 DEG C of temperature, 80%~85% environment of humidity, promote silkworm seed embryo of silkworms
Smoothly it is developed to hatching, normally collects ants and raise.
The time of protection described in step (1) is preferably 40 hours.
The temperature of placement described in step (2) is preferably 15 DEG C.
The time of placement described in step (2) is preferably 2 hours.
Stored refrigerated temperature described in step (3) is preferably 4~6 DEG C；More preferably 5 DEG C.
The temperature of placement described in step (4) is preferably 15 DEG C.
When the time of placement described in step (4) is generally 27~30 days, silkworm seed embryo of silkworms can just be developed to body pigmentation stage.
The method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra is in no diapause polyvoltinism silkworm germplasm
Application in resource conservation.
The present invention has the following advantages and effects with respect to the prior art:
(1) method provided by the invention can be by conventional annual 7- to the child care algebra of no diapause polyvoltinism silkworm germ plasm resource
In 8 generations, reduced to for 4 generations, greatly reduced raising number, labor intensity and feeding cost, at the same the main morphological features of silkworm germplasm and
Economic characters are not significantly affected.
(2) stored refrigerated to the progress of no diapause polyvoltinism silkworm germ plasm resource using this method, in tropical and subtropical zone silkworm area
Mulberry tree dormant period in winter (mid-February mid-December -) can be avoided completely, can also be avoided in other mulberry tree dormant periods longer area
One period, and can guarantee the Spring Festival holiday of labour, so that it is enjoyed the reasonable time of having a rest.
(3) this method provides the silkworm for meeting Labor-saving high efficiency with reference to approach for more effectively preservation polyvoltinism resource
Modern yard feeding pattern, scientific research and teaching unit in store no diapause polyvoltinism silkworm germ plasm resource have important reference price
Value and application prospect.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Following silkworm egg resource can be commercially available from Guangdong silkworm and mulberry germplasm resource bank: 305, Qiongshan Hainan, it is safe stop, 7018,
301, P50 is raw.
Embodiment 1
A method of it reduces without diapause polyvoltinism silkworm germ plasm resource child care algebra, includes the following steps:
(1) without diapause polyvoltinism silkworm germ plasm resource silkworm seed after giving birth in temperature is 25~26 DEG C, relative humidity is
It is protected 40 hours in the environment of 75%~80%.
(2) 15 DEG C of environment is next moved to place 2 hours；
(3) environment stored refrigerated 28 days of 5 DEG C are then moved to；
(4) 15 DEG C of environment is then moved to place to the silkworm seed development of embryo to body pigmentation stage；305 and Qiongshan Hainan need 29 days,
Thailand stops and 7018 need 28 days, and 301 and P50 is raw to need 27 days.
(5) it takes out, carries out hiding black processing under 27~28 DEG C of temperature, 80%~85% environment of humidity, promote silkworm seed embryo of silkworms
Smoothly it is developed to hatching, normally collects ants and raise.
(6) preservation effect is analyzed, the results are shown in Table 1.
It is compared simultaneously with the effect of conventional method used at present, the step of conventional method is as follows:
(A) without diapause polyvoltinism silkworm germ plasm resource silkworm seed after giving birth in temperature is 25~26 DEG C, relative humidity is
It is protected 40 hours in the environment of 75%~80%；
(B) it next moves to 15 DEG C of environment and places transition in 2 hours；
(C) environment stored refrigerated 28 days of 5 DEG C are then moved to；
(D) it next moves to 15 DEG C of environment and places transition in 2 hours, be subsequently placed in 27~28 DEG C of environment and hasten the hatching of silkworms 7 days；
(E) it takes out, carries out hiding black processing under 27~28 DEG C of temperature, 80%~85% environment of humidity, promote silkworm seed embryo of silkworms
Smoothly it is developed to hatching, normally collects ants and raise.
(F) preservation effect is analyzed, the results are shown in Table 1.
1 new method of table and conventional method, which examine the t of the stored refrigerated effect of no diapause polyvoltinism silkworm germplasm silkworm seed, divides
Analysis
Note: " * " indicates significant difference；T (4,0.05)=2.776, t (4,0.01)=4.604
As it can be seen from table 1 the present invention, compared with conventional method, silkworm main morphological features are unchanged, Hatching Rate of Silkworm Eggs
Decline unobvious, worm pupa unifies vital rates, cocoon weight, cocoon shell weight, Cocoon layer ratio etc. there are no significant difference, the i.e. master of silkworm germplasm
Morphological feature and economic characters is wanted not to be significantly affected.
The time that method provided by the invention can save no diapause polyvoltinism cold storage of silkworm egg extends 20~22 days.To,
By annual 7~8 generation of conventional method can be reduced to 4 generations to the child care algebra of no diapause polyvoltinism silkworm germ plasm resource, greatly reduced
Raising number and labor intensity, in tropical and subtropical zone silkworm area can avoid mulberry tree dormant period in winter (in -2 months mid-Decembers completely
Ten days) and the manpower shortage phase in spring vacation.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (7)
1. a kind of method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra, it is characterised in that include the following steps:
(1) without diapause polyvoltinism silkworm germ plasm resource silkworm seed after giving birth in temperature be 25~26 DEG C, relative humidity be 75%~
It is protected 38~42 hours in the environment of 80%；
(2) 14~16 DEG C of environment is next moved to place 1 .5~2 .5 hours；
(3) environment stored refrigerated 25~28 days of 4~6 DEG C are then moved to；
(4) 14~16 DEG C of environment is then moved to place to the silkworm seed development of embryo to body pigmentation stage；The time of the placement be 27~
30 days；
(5) it takes out, carries out hiding black processing under 27~28 DEG C of temperature, 80%~85% environment of humidity, promote silkworm seed embryo of silkworms smooth
It is developed to hatching, normally collects ants and raise.
2. method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra according to claim 1, feature exist
In: the time of protection described in step (1) is 40 hours.
3. method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra according to claim 1, feature exist
In: the temperature of placement described in step (2) is 15 DEG C.
4. method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra according to claim 1, feature exist
In: the time of placement described in step (2) is 2 hours.
5. method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra according to claim 1, feature exist
In: stored refrigerated temperature described in step (3) is 5 DEG C.
6. method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra according to claim 1, feature exist
In: the temperature of placement described in step (4) is 15 DEG C.
7. any one of claim 1~6 method of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra is without stagnant
Educate the application in polyvoltinism silkworm Germ-plasma resources protection.
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| CN107372458A (en) * | 2017-07-15 | 2017-11-24 | 合肥市聚丰制丝有限责任公司 | A kind of silkworm eggs store method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1286022A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Slightly freezing method for storing silkworm ova |
| CN1370404A (en) * | 2002-03-06 | 2002-09-25 | 张殿明 | Silkworm egg cold storage incubation ant collecting method |
| CN1631128A (en) * | 2004-12-28 | 2005-06-29 | 张以俭 | Method for treating silkworm egg comprising shot-term refrigeration, high temperature impact and acid immersion |
| JP4273225B2 (en) * | 2003-03-05 | 2009-06-03 | 独立行政法人農業生物資源研究所 | Preservation of silkworm fertilized eggs |
| KR20160051419A (en) * | 2014-11-03 | 2016-05-11 | 대한민국(농촌진흥청장) | Method For Storage Of Osmia pedicornis for Adult Emergence At Once |
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- 2016-11-08 CN CN201610979869.4A patent/CN106508827B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1286022A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Slightly freezing method for storing silkworm ova |
| CN1370404A (en) * | 2002-03-06 | 2002-09-25 | 张殿明 | Silkworm egg cold storage incubation ant collecting method |
| JP4273225B2 (en) * | 2003-03-05 | 2009-06-03 | 独立行政法人農業生物資源研究所 | Preservation of silkworm fertilized eggs |
| CN1631128A (en) * | 2004-12-28 | 2005-06-29 | 张以俭 | Method for treating silkworm egg comprising shot-term refrigeration, high temperature impact and acid immersion |
| KR20160051419A (en) * | 2014-11-03 | 2016-05-11 | 대한민국(농촌진흥청장) | Method For Storage Of Osmia pedicornis for Adult Emergence At Once |
Non-Patent Citations (1)
| Title |
|---|
| 无滞育多化性蚕卵的冷藏保种研究;沈卫德等;《蚕业科学》;19881231;第14卷(第4期);第226-227页 * |
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