CN106508662A - Pollination method in angiosperm crop breeding process in facility - Google Patents
Pollination method in angiosperm crop breeding process in facility Download PDFInfo
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- CN106508662A CN106508662A CN201610947415.9A CN201610947415A CN106508662A CN 106508662 A CN106508662 A CN 106508662A CN 201610947415 A CN201610947415 A CN 201610947415A CN 106508662 A CN106508662 A CN 106508662A
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- angiosperm
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of plant crop breeding, concretely relates to a pollination method in an angiosperm crop breeding process in facility, and concretely relates to the pollination method in a liliaceae and cruciferous crop breeding process. The pollination method selects bombus colony of bombus for crop pollination, and is especially suitable for suitable for liliaceae and cruciferous crop pollination. The bombus cannot depend on the bee colony, pollination can be completed by individual movement, the body is large, the body hair is heavy, a lot of pollen can be carried, and the pollination effect is excellent.
Description
Technical field
The invention belongs to plant crop reproduction technique field, and in particular to angiosperm crop breeding process in a kind of facility
In pollinating method, be more particularly to the pollinating method in Liliaceae and crop in cruciferae breeding process in a kind of facility.
Background technology
Angiosperm (Angiosperm) also known as green flowering plant, are frequently referred to Angiospermae on taxonomy.About 1
Before 100000000 years, gymnosperm is from prosperity to decline so that angiosperm is developed, and becomes and is distributed the most wide, plant of most species on the earth
Thing.Angiosperm also cries phanerogam, flowering plant, and they possess real flower, and the flower of these beauties is that they raise up seed
Vitals, and they are different from the marked feature of gymnosperm and other plant.Angiosperm is that plant kingdom is highest
A class, and on the earth, most perfect, adaptability is most strong, the plant that occurs the latest, and since the Cenozoic, they are in the earth
On account for absolute advantagess.Angiosperm has category more than 10,000, about 300,000 kinds, constitutes about botanic half.They come in every shape, bag
Include tall and big arbor, short and small shrub and some herbaceous plant.Angiosperm can have so numerous species, have extremely extensive
Adaptability, this is inseparable with its complex structure, improvement, particularly the spy of the structure and reproductive process of organ of multiplication
Point, there is provided it adapts to, resists the internal condition of various environment, makes it in struggle for existence, the contradiction struggle process of natural selection
In, new variation is constantly produced, new species are produced.
, also known as breed improvement, its breeding method is closely related with the propagation method of crop, common are selfing for crop breeding
Crop breeding and outcrossing crop breeding.Both breeding modes require specific maternal and paternal parent, and this is accomplished by educating
During kind, crop is isolated from the outside, prevents insecticide from bringing the pollen of other strains into, cause string powder phenomenon.Current facility
The Pollinating Insect commonly used in crop breeding has fly, wall honeybee, Apiss and bumblebee.Wherein, fly easily obtains, low cost, but awards
Powder is extremely inefficient, and its pollination is mainly completed by the irregular activity of a large amount of flies, and which is only suitable for using in Liliaceae pollination, should
It is narrower with scope;Wall honeybee is adapted to orchard pollination, and its pollination activity is also required to more than 12 DEG C, and its upper strata often in the facility is lived
Dynamic, poor to the Pollination Effect of bottom flower, which is only suitable for using in the pollination of Cruciferae section, and range of application is narrower;Hive gesture is big,
Phototaxis is strong, easily hits canopy, high to temperature requirement, and temperature stays in less than 17 DEG C and adopts powder, the range of application in protected crop breeding
It is minimum;Although bumblebee pollination group is adapted to pollination in facility, if pollinated in the facility of small area, just occur due to flower
Powder amount is not enough, caused excessive pollination phenomenon, and strong bumblebee worker bee visits the floral organs such as flower, destruction stigma repeatedly, causes abortion, single
, in the case of the stimulation of no brood pheromone, visit flower desire is not strong, and pollination efficiency is low for only bumblebee worker bee.So, in facility
The pollination of breeding crop is there is still a need for a more efficient, easier method.
The content of the invention
For this purpose, the technical problem to be solved is to provide in a kind of facility during angiosperm crop breeding
Pollinating method, the pollinating method in being especially adapted for use in Liliaceae and crop in cruciferae breeding process, to solve existing skill
The low problem of Liliaceae and crop in cruciferae pollination rate in art.
For solving above-mentioned technical problem, the pollination side in facility of the present invention during angiosperm crop breeding
Method, is pollinated for entomophila using the male honeybee of bumblebee.
The male honeybee is 35-65 with the quantity ratio of the plant:20.
Preferably, when, when the plant that pollinates is less than 20 plants, the male honeybee is 55-65 with the quantity ratio of the plant:20.
When, when the plant that pollinates is less than 20 plants, the male honeybee is 60 with the quantity ratio of the plant:20.
The male honeybee is pollinated for release by several times.
Preferably, when, when the plant that pollinates is higher than 20 plants, the male honeybee is 35-45 with the quantity ratio of the plant:20.
When, when the plant that pollinates is higher than 20 plants, the male honeybee is 40 with the quantity ratio of the plant:20.
Preferably, when, when the plant that pollinates is 20 plants, the male honeybee is 45-55 with the quantity ratio of the plant:20.
When, when the plant that pollinates is 20 plants, the male honeybee is 50 with the quantity ratio of the plant:20.
The angiosperm includes Liliaceae crop and crop in cruciferae.
Pollinating method during angiosperm crop breeding of the present invention, is entered row crop and is awarded from the male honeybee of bumblebee
Powder, as the male honeybee of bumblebee is taken food 1-2 days after emergence in nest, can go out nest, and independent action is sought after eating and drinking to one's heart's content
Virgin mating is looked for, is no longer gone back to the nest, be adapted to individually activity, therefore be adapted to crop pollination and be used.Importantly, due to bumblebee
Male honeybee only gathers nectar and regains one's strength of body, and does not gather pollen, can access and not have polliniferous androecium abortion flower, and due to which
Chaeta is more, can carry many pollen, takes powder amount greatly, and its Pollination Effect is fabulous;And due in the input worm that comes into bloom
Spend few honeybee many at the beginning of matchmaker, therefore bad flower will not be stung;And bumblebee phototaxis is poor, substantially without hitting canopy phenomenon;And male honeybee is without tailpin,
People is not bitten, can factorial praluction.Pollinating method of the present invention is particularly suitable for Liliaceae crop and crop in cruciferae pollination
It is used, its Pollination Effect is more preferably.
Specific embodiment
Embodiment 1
50 plants of Bulbus Allii Cepae plant is chosen, and which is planted pollination experiment detection to be carried out in a conventional manner with the male honeybee 100 of bumblebee
The pollination rate of strain is 85.23%.
50 plants of the Bulbus Allii Cepae plant of the equal development of the same area is chosen, 200 carry out conventional pollination with fly, detect which is awarded
Powder rate is only 53.0%.
Embodiment 2
10 plants of Plantula Brassicae chinensis plant is chosen, and artificial pollination experiment is carried out according to existing usual manner, is detected that its setting percentage is
85%.
10 plants of the Plantula Brassicae chinensis plant of the equal development of the same area is chosen, conventional awarding is carried out with the male honeybee 30 of bumblebee
Powder, equivalent is put into pollination to the male honeybee of the bumblebee at twice, detects that its setting percentage is 94%.
Embodiment 3
20 plants of Fructus Solani melongenae plant is chosen, spontaneous pollination is carried out according to existing usual manner, detect that its fructufy situation is 36.
20 plants of the Fructus Solani melongenae plant of the equal development of the same area is chosen, conventional pollination is carried out with the male honeybee 50 of bumblebee,
Detect that its fructufy situation is 48.
Embodiment 4
50 plants of Chinese cabbage plant is chosen, and artificial supplementary pollination is carried out according to existing usual manner, is detected the effective of its plant
Pod number is 794.8.
50 plants of the Chinese cabbage plant of the equal development of the same area is chosen, conventional awarding only is carried out with the male honeybee 100 of bumblebee
Powder, detects that effective pod number of its plant is 868.3.
Bumblebee be can be seen that from above-mentioned contrast test, and (strain tree is few, needs to prevent from going here and there between strain species more for small-scale
Powder) test breeding is particularly suitable, is easy to go here and there the low defect of powder, fly pollination efficiency compared to artificial pollination, using the hero of bumblebee
Property honeybee is pollinated, especially the huge advantage of itself.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description other multi-forms change or
Change.There is no need to be exhaustive to all of embodiment.And thus it is extended obvious change or
Among changing still in the protection domain of the invention.
Claims (10)
1. the pollinating method in a kind of facility during angiosperm crop breeding, it is characterised in that using the male honeybee of bumblebee
Pollinated for entomophila.
2. the pollinating method in facility according to claim 1 during angiosperm crop breeding, it is characterised in that institute
The quantity ratio that male honeybee is stated with the plant is 35-65:20.
3. the pollinating method in facility according to claim 2 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is less than 20 plants, the male honeybee is 55-65 with the quantity ratio of the plant:20.
4. the pollinating method in facility according to claim 3 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is less than 20 plants, the male honeybee is 60 with the quantity ratio of the plant:20.
5. the pollinating method in the facility according to claim 3 or 4 during angiosperm crop breeding, its feature exist
In the male honeybee is pollinated for release by several times.
6. the pollinating method in facility according to claim 2 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is higher than 20 plants, the male honeybee is 35-45 with the quantity ratio of the plant:20.
7. the pollinating method in facility according to claim 6 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is higher than 20 plants, the male honeybee is 40 with the quantity ratio of the plant:20.
8. the pollinating method in facility according to claim 2 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is 20 plants, the male honeybee is 45-55 with the quantity ratio of the plant:20.
9. the pollinating method in facility according to claim 8 during angiosperm crop breeding, it is characterised in that when
When the plant that pollinates is 20 plants, the male honeybee is 50 with the quantity ratio of the plant:20.
10. the pollinating method in the facility according to any one of claim 1-9 during angiosperm crop breeding, which is special
Levy and be, the angiosperm includes Liliaceae crop and crop in cruciferae in facility.
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CN201610947415.9A CN106508662A (en) | 2016-11-02 | 2016-11-02 | Pollination method in angiosperm crop breeding process in facility |
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CN201610947415.9A CN106508662A (en) | 2016-11-02 | 2016-11-02 | Pollination method in angiosperm crop breeding process in facility |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102334445A (en) * | 2010-07-16 | 2012-02-01 | 山东省潍坊市农业科学院 | Pollination method for purity-conservation Chinese cabbage breeding material |
CN103651107A (en) * | 2013-12-21 | 2014-03-26 | 山东鑫诚现代农业科技有限责任公司 | Bumblebee artificial-propagation subculturing pollination technology |
CN105409757A (en) * | 2015-11-05 | 2016-03-23 | 石大勇 | Bumblebee pollination method for facility tomatoes |
CN205389849U (en) * | 2016-03-08 | 2016-07-27 | 山东省农业科学院植物保护研究所 | Underground case of pollinating |
-
2016
- 2016-11-02 CN CN201610947415.9A patent/CN106508662A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102334445A (en) * | 2010-07-16 | 2012-02-01 | 山东省潍坊市农业科学院 | Pollination method for purity-conservation Chinese cabbage breeding material |
CN103651107A (en) * | 2013-12-21 | 2014-03-26 | 山东鑫诚现代农业科技有限责任公司 | Bumblebee artificial-propagation subculturing pollination technology |
CN105409757A (en) * | 2015-11-05 | 2016-03-23 | 石大勇 | Bumblebee pollination method for facility tomatoes |
CN205389849U (en) * | 2016-03-08 | 2016-07-27 | 山东省农业科学院植物保护研究所 | Underground case of pollinating |
Non-Patent Citations (7)
Title |
---|
KATHERINE L.OSTEVIK ET AL.: "POLLINATION POTENTIAL OF MALE BUMBLE BEES (BOMBUS IMPATIENS):MOVEMENT PATTERNS AND POLLEN-TRANSFER EFFICIENCY", 《JOURNAL OF POLLINATION ECOLOGY》 * |
KYEONG YONG LEE ET AL.: "The Comparative Study of Pollination with Flies and Bumblebee(Bombus terrestris L.) in Different Onion Cultivars", 《SOUTH KOREA APPLIED ENTOMOLOGY REGULAR MEETING AND SPRING ACADEMIC CONFERENCE》 * |
刘春梅等: "熊蜂授粉技术在设施草莓上的应用", 《新疆农业科技》 * |
安建东等: "熊蜂为温室茄子授粉试验", 《中国养蜂》 * |
董捷等: "不同蜂传粉对设施桃果实生长发育和品质的影响", 《中国生态农业学报》 * |
蔡继红: "熊蜂及其在设施农业生产中的作用", 《新疆农业科技》 * |
黄家兴等: "设施作物熊蜂授粉关键技术及其应用前景", 《现代农业科技》 * |
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Application publication date: 20170322 |