CN106508173B - Method for promoting dragon fruit seed germination - Google Patents
Method for promoting dragon fruit seed germination Download PDFInfo
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- CN106508173B CN106508173B CN201610947681.1A CN201610947681A CN106508173B CN 106508173 B CN106508173 B CN 106508173B CN 201610947681 A CN201610947681 A CN 201610947681A CN 106508173 B CN106508173 B CN 106508173B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for promoting dragon fruit seed germination, which comprises the step of soaking dragon fruit seeds for 6-24 hours by using a seed soaking agent before the dragon fruit seeds are germinated and cultured, wherein the seed soaking agent contains gibberellin with the concentration of 50-250mg/L, kinetin with the concentration of 5-30mg/L and DA-6 with the concentration of 5-15 mg/L. The method improves the activity of the dragon fruit seeds, and has high germination uniformity and good seedling quality.
Description
Technical Field
The invention relates to the technical field of plant seed germination. More particularly, the invention relates to a method for promoting the germination of pitaya seeds.
Background
Pitaya (Latin article name: Hylocereus undatus Britt), also known as red dragon fruit, Xianmei fruit, and Yulong dragon fruit. Is a plant of Cactaceae, genus Petasites. The dragon fruit is tropical and subtropical fruit, and is introduced into places such as costa rica, guatemala, panama, ecuador, copa, columbia, etc. in central america of origin, and then introduced into southeast asian countries such as vietnam and thailand, Taiwan provinces in china, and provinces such as Hainan, Guangxi, Guangdong, Fujian, Yunnan, etc. in continental china. The pitaya is rich in nutrition and unique in function, contains plant albumin and anthocyanin which are few in common plants, abundant vitamins and water-soluble dietary fibers, belongs to cold fruits, and is ripe in summer and autumn, sweet in taste and juicy in a natural state. The propagation mode of the dragon fruit mainly comprises cuttage and seed sowing, and due to the reasons of small seeds, hard seed shells, difficult water absorption and the like of the dragon fruit, the phenomena of slow seed germination, irregular germination and low germination rate appear after sowing, and the dragon fruit seeds are easy to adhere to pulp, so that the mildew phenomenon is easily caused in the germination, and the germination effect is influenced.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
The invention also aims to provide a method for promoting the germination of the dragon fruit seeds, which improves the germination rate of the dragon fruit seeds, shortens the germination time and solves the problems of weak germination capacity, low germination rate and weak seedlings through seed soaking treatment.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for promoting germination of dragon fruit seeds, comprising soaking dragon fruit seeds for 6-24 hours with a seed soaking agent containing gibberellin at a concentration of 50-250mg/L, kinetin at a concentration of 5-30mg/L, and DA-6 at a concentration of 5-15mg/L before the dragon fruit seeds are germinated and cultured.
Preferably, in the method for promoting the germination of the pitaya seeds, the concentration of gibberellin in the seed soaking agent is 150mg/L, the concentration of kinetin in the seed soaking agent is 8mg/L, DA-6 is 10mg/L, and the soaking time of the seed soaking agent is 12 hours.
Preferably, the method for promoting the germination of the dragon fruit seeds comprises the following specific steps of firstly disinfecting the dragon fruit seeds before soaking the dragon fruit seeds in the seed soaking agent: placing the pretreated dragon fruit seeds in 0.5 mass percent of bleaching powder aqueous solution for 10-30min, then placing the dragon fruit seeds in 10 mass percent of trisodium phosphate aqueous solution for soaking for 10-20min, cleaning for 3-4 times, and then carrying out ultraviolet radiation disinfection treatment for 30-60min under the condition that the ultraviolet wavelength is 210-328 nm.
Preferably, the method for promoting the germination of the dragon fruit seeds comprises the steps of putting the dragon fruit seeds into a transparent glass culture dish with filter paper, and placing the dragon fruit seeds into a constant-temperature incubator for culture, wherein the temperature of the constant-temperature incubator is 20-32 ℃, the humidity of the incubator is 75-85%, and the culture time is 10-30 days.
Preferably, in the method for promoting the germination of the dragon fruit seeds, the wavelength of the ultraviolet radiation disinfection treatment is 250nm, and the time is 40 min.
Preferably, the method for promoting the germination of the dragon fruit seeds further comprises the steps of adding nano zinc oxide with the concentration of 3mg/L and a cattail pollen water extract with the concentration of 5mg/L into the seed soaking agent, mixing the cattail pollen and water according to the mass ratio of 1:25, boiling with strong fire, decocting with slow fire for 5 hours, and filtering gauze of 100 meshes to obtain the cattail pollen water extract;
the soaking treatment of the dragon fruit seeds by the seed soaking agent comprises the following steps: firstly soaking the dragon fruit seeds soaked by the seed soaking agent and the seed soaking agent for 5h at 4 ℃, then placing the dragon fruit seeds in a 200-plus-300 gauss magnetic field for magnetization treatment for 3h, and finally placing the dragon fruit seeds in a 30 ℃ soaking treatment for 4h, wherein the soaking treatments at 4 ℃ and 30 ℃ are both carried out under the irradiation of ultraviolet rays with the wavelength of 250-plus-300 nm.
Preferably, the pretreatment is to collect ripe pitaya fruits, peel the ripe pitaya fruits, cut the ripe pitaya fruits into small pieces, place the pitaya fruits in a screen bag with the aperture smaller than 20 meshes, rub and wash the pitaya fruits in running water, separate pulp and seeds of the pitaya fruits, clean the separated seeds of the pitaya fruits, and place the seeds in a ventilated place for drying in the shade for later use.
The invention at least comprises the following beneficial effects:
1) according to the method for promoting the germination of the dragon fruit seeds, the germination of the dragon fruit seeds is remarkably promoted by soaking the seeds in the seed soaking agent with proper concentration for proper time, so that the germination rate of the dragon fruit seeds is up to more than 90%, the germination rate of the dragon fruit seeds can be remarkably improved while the mildew phenomenon of the dragon fruit seeds is reduced, the problems of weak germination capacity, low germination rate and thin and weak seedlings are solved, the operation is simple, the cost is low, the manual operation is easy, and a foundation is provided for the seeding, seedling raising and breeding work of the dragon fruit seeds in future;
2) the invention also carries out soaking treatment by a seed soaking agent containing nano zinc oxide with proper content and a cattail pollen aqueous extract, combines soaking and magnetic field magnetization treatment, further improves the germination rate of the dragon fruit seeds, and shortens the germination time, wherein the nano zinc oxide has large specific surface area, can promote the absorption of the dragon fruit seeds to other components, and can excite active oxygen and kill germ viruses under the irradiation of ultraviolet light because the nano zinc oxide is a photocatalyst, and the cattail pollen aqueous extract can enhance the hypoxia resistance of the dragon fruit seeds, improve the respiration of the seeds, enhance the germination potential, shorten the germination time and promote the germination of the seeds.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail with reference to specific examples, so that those skilled in the art can implement the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
Example 1:
a method for promoting germination of dragon fruit seeds comprises the step of soaking the dragon fruit seeds for 6 hours by using a seed soaking agent before germination and culture of the dragon fruit seeds, wherein the seed soaking agent contains gibberellin with the concentration of 50mg/L, kinetin with the concentration of 5mg/L and DA-6 with the concentration of 5 mg/L. As used herein, the content of each component in 1L of seed soaking agent is as follows: the gibberellin content in each 1L seed soaking agent is 50 mg.
DA-6 is diethylaminoethanol caproate.
Example 2:
a method for promoting germination of dragon fruit seeds comprises the step of soaking the dragon fruit seeds for 24 hours by using a seed soaking agent before germination and culture of the dragon fruit seeds, wherein the seed soaking agent contains gibberellin with the concentration of 250mg/L, kinetin with the concentration of 30mg/L and DA-6 with the concentration of 15 mg/L.
Example 3:
a method for promoting germination of dragon fruit seeds comprises the step of soaking the dragon fruit seeds for 12 hours by using a seed soaking agent before germination and culture of the dragon fruit seeds, wherein the seed soaking agent contains gibberellin with the concentration of 150mg/L, kinetin with the concentration of 8mg/L and DA-6 with the concentration of 10 mg/L.
Example 4:
on the basis of embodiment 3, the method for promoting the germination of the dragon fruit seeds comprises the following specific steps of disinfecting the dragon fruit seeds before soaking the dragon fruit seeds in the seed soaking agent: placing the pretreated dragon fruit seeds in 0.5 mass percent of bleaching powder aqueous solution for 10-30min, then placing the dragon fruit seeds in 10 mass percent of trisodium phosphate aqueous solution for soaking for 10-20min, cleaning for 3-4 times, and then carrying out ultraviolet radiation disinfection treatment for 30-60min under the condition that the ultraviolet wavelength is 210-328 nm. The optimal conditions are that the wavelength of the ultraviolet radiation disinfection treatment is set to be 250nm, and the ultraviolet radiation disinfection treatment time is 40 min.
The method for promoting the germination of the dragon fruit seeds is characterized in that the dragon fruit seeds are placed in a transparent glass culture dish with filter paper and are placed in a constant-temperature incubator for culture, the temperature of the constant-temperature incubator is 20-32 ℃, the humidity of the incubator is 75-85%, and the culture time is 10-30 days.
The method for promoting the germination of the dragon fruit seeds comprises the steps of collecting mature dragon fruit, peeling, cutting into small pieces, placing the small pieces into a screen bag with the aperture smaller than 20 meshes, rubbing and washing under running water, separating dragon fruit pulp and seeds, cleaning the separated dragon fruit seeds, and then placing the cleaned dragon fruit seeds in a ventilation place to be dried in the shade for later use.
Example 5:
on the basis of embodiment 4, the method for promoting dragon fruit seed germination further comprises the steps of adding nano zinc oxide with the concentration of 3mg/L and cattail pollen aqueous extract with the concentration of 5mg/L into the seed soaking agent, mixing the cattail pollen and water according to the mass ratio of 1:25, boiling with strong fire, decocting with slow fire for 5 hours, and filtering gauze of 100 meshes to obtain the cattail pollen aqueous extract;
the soaking treatment of the dragon fruit seeds by the seed soaking agent comprises the following steps: firstly soaking the dragon fruit seeds soaked by the seed soaking agent and the seed soaking agent for 5h at 4 ℃, then placing the dragon fruit seeds in a 200-plus-300 gauss magnetic field for magnetization treatment for 3h, and finally placing the dragon fruit seeds in a 30 ℃ soaking treatment for 4h, wherein the soaking treatments at 4 ℃ and 30 ℃ are both carried out under the irradiation of ultraviolet rays with the wavelength of 250-plus-300 nm.
To illustrate the effects of the present invention, the inventors of the present invention conducted studies on examples 4 and 5, the conventional method (i.e., soaking dragon fruit seeds in bleaching powder and trisodium phosphate aqueous solution in the conditions of example 4, washing, and then directly culturing the seeds by germination culture method of example 4, specifically, see example 4), and some of the other beards3Soaking seed to promote dragon fruit seed germination to obtain control group with proper soaking concentration (using GA with concentration of 400 mg/L)3Soaking seeds in an aqueous solution for 12h as in example 3, and soaking seeds in a bleaching powder and trisodium phosphate aqueous solution as in example 4 in a control group, and performing germination culture in the same manner as in a conventional method) to germinate the dragon fruit seeds from the same source, and determining the germination vigor, the germination rate and the fresh weight of each seedling of the dragon fruit seeds according to the national standard of crop seed inspection, wherein the results are shown in table 1 below,
germination vigor (%) — number of germinated seeds/number of test seeds on day 8 × 100%;
germination rate (%) — number of germinated seeds/number of test seeds on day 14 × 100%;
the fresh weight of each seedling is 10 random seedlings are taken to determine the fresh weight of each seedling/10.
TABLE 1 comparison of the germination results of dragon fruit seeds of different examples
| Processing method | Germination potential/%) | Germination rate/% | Fresh weight of single seedling/mg |
| Conventional methods | 17.8 | 23.2 | 5.9 |
| Control group | 56.8 | 48.5 | 11.8 |
| Example 4 | 78.6 | 91.8 | 19.6 |
| Example 5 | 84.9 | 97.5 | 24.7 |
As can be seen from table 1, in example 4, compared with the conventional method and the method of the control group, the germination rate and the germination vigor of the dragon fruit seeds are obviously improved, the fresh weight of the individual seedlings is also obviously increased, the germination vigor refers to the germination speed and the germination uniformity of the seeds within a specified time, which indicates the vitality of the seeds, and the fresh weight of the seedlings reflects the vitality level of the seeds to a certain extent.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (5)
1. A method for promoting the germination of dragon fruit seeds is characterized in that the dragon fruit seeds are soaked for 12 hours by using a seed soaking agent before the germination and culture of the dragon fruit seeds, wherein the seed soaking agent contains gibberellin with the concentration of 150mg/L, kinetin with the concentration of 8mg/L and DA-6 with the concentration of 10 mg/L;
the seed soaking agent also contains nano zinc oxide with the concentration of 3mg/L and a cattail pollen aqueous extract with the concentration of 5mg/L, the solvent of the seed soaking agent is magnetized water, the cattail pollen aqueous extract is obtained by mixing cattail pollen and water according to the mass ratio of 1:25, boiling with strong fire, decocting with slow fire for 5 hours, and filtering gauze with 100 meshes;
the soaking treatment of the dragon fruit seeds by the seed soaking agent comprises the following steps: firstly soaking the dragon fruit seeds soaked by the seed soaking agent and the seed soaking agent for 5h at 4 ℃, then placing the dragon fruit seeds in a 200-plus-300 gauss magnetic field for magnetization treatment for 3h, and finally placing the dragon fruit seeds in a 30 ℃ soaking treatment for 4h, wherein the soaking treatments at 4 ℃ and 30 ℃ are both carried out under the irradiation of ultraviolet rays with the wavelength of 250-plus-300 nm.
2. The method for promoting the germination of the dragon fruit seeds as claimed in claim 1, wherein the dragon fruit seeds are disinfected before being soaked with the seed soaking agent, and the method comprises the following specific steps: placing the pretreated dragon fruit seeds in 0.5 mass percent of bleaching powder aqueous solution for 10-30min, then placing the dragon fruit seeds in 10 mass percent of trisodium phosphate aqueous solution for soaking for 10-20min, cleaning for 3-4 times, and then carrying out ultraviolet radiation disinfection treatment for 30-60min under the condition that the ultraviolet wavelength is 210-328 nm.
3. The method for promoting the germination of the dragon fruit seeds as claimed in claim 1, wherein the method for cultivating the dragon fruit seeds by germination specifically comprises the steps of putting the dragon fruit seeds into a transparent glass culture dish with filter paper, and putting the dragon fruit seeds into a constant-temperature incubator for cultivation, wherein the temperature of the constant-temperature incubator is 20-32 ℃, the humidity of the incubator is 75-85%, and the cultivation time is 10-30 days.
4. The method for promoting germination of dragon fruit seeds as claimed in claim 2, wherein the ultraviolet radiation disinfection treatment is performed at a wavelength of 250nm for a period of 40 min.
5. The method for promoting germination of dragon fruit seeds as claimed in claim 2, wherein the pre-treatment specifically refers to collecting mature dragon fruit, peeling, cutting into small pieces, placing the small pieces in a sieve bag with a pore size smaller than 20 meshes, rubbing and washing under running water, separating dragon fruit pulp and seeds, cleaning the separated dragon fruit seeds, and then placing the cleaned dragon fruit seeds in a ventilated place for drying in the shade for later use.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107548610A (en) * | 2017-09-28 | 2018-01-09 | 广西沙田仙人滩农业投资有限公司 | A kind of sterile quick pregermination method of dragon fruit seed |
| CN108293357A (en) * | 2017-09-30 | 2018-07-20 | 广西吉朋投资有限公司 | A kind of high-quality and efficient method for culturing seedlings of dragon fruit |
| CN108040845A (en) * | 2017-11-27 | 2018-05-18 | 余雪平 | A kind of method for culturing seedlings of oil Paeonia suffruticosa seed |
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| CN108370927A (en) * | 2018-01-31 | 2018-08-07 | 平南县正达农业发展有限公司 | A kind of type of rearing of dragon fruit |
| CN108307948B (en) * | 2018-01-31 | 2020-04-24 | 平南县正达农业发展有限公司 | Mechanized planting method for dragon fruits |
| CN108718601B (en) * | 2018-05-23 | 2021-04-30 | 广西壮族自治区药用植物园 | Method for promoting germination of asparagus cochinchinensis seeds |
| CN108575185A (en) * | 2018-05-25 | 2018-09-28 | 合肥慧谷农业科技有限公司 | A method of improving Germination of Watermelon Seed rate |
| CN109041650A (en) * | 2018-11-01 | 2018-12-21 | 界首市王集镇毛毛家庭农场 | A kind of method for treating seeds improving crowndaisy chrysanthemum yield and quality |
| CN111527823B (en) * | 2020-05-08 | 2021-08-27 | 广西壮族自治区药用植物园 | Method for promoting germination of parasitic loranthus seeds |
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