CN106404472A - Experiment device and method for in-situ collection of root system volatiles - Google Patents
Experiment device and method for in-situ collection of root system volatiles Download PDFInfo
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- CN106404472A CN106404472A CN201610995674.9A CN201610995674A CN106404472A CN 106404472 A CN106404472 A CN 106404472A CN 201610995674 A CN201610995674 A CN 201610995674A CN 106404472 A CN106404472 A CN 106404472A
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- top cover
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- volatile matter
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- 238000011065 in-situ storage Methods 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title abstract description 6
- 239000003039 volatile agent Substances 0.000 title abstract 4
- 241000196324 Embryophyta Species 0.000 claims abstract description 35
- 239000007789 gas Substances 0.000 claims description 42
- 238000003556 assay Methods 0.000 claims description 13
- 239000011159 matrix material Substances 0.000 claims description 6
- 238000010998 test method Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 4
- 229920000742 Cotton Polymers 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 239000012855 volatile organic compound Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229940023032 activated charcoal Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 244000038280 herbivores Species 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000010309 melting process Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to an experiment device for in-situ collection of root system volatiles. The experiment device comprises a box for plant seedling growth, four side walls and the bottom of the box are closed, a left top cover and a right top cover which are openable and closeable are arranged at the top of the box, a planting groove for plant seedlings to penetrate is formed between the left top cover and the right top cover, the bottom of the box is externally connected with an air incoming pipeline, a gas flowmeter, an air purifier and an air compressor sequentially through an air incoming hole, and the top of the box is externally connected with an air outgoing pipeline and a gas collection bag sequentially through an air outgoing hole. The invention further relates to an experiment method for in-situ collection of the root system volatiles. The experiment device is simple in integral structure, low in manufacturing cost, convenient in material getting and manufacturing and especially capable of instantaneously, continuously and accurately collecting and analyzing feature indexes like number and type of the root system volatiles without causing damage to root systems under real cultivation conditions.
Description
Technical field
The present invention relates to a kind of assay device for collecting root system volatile matter in situ and method.
Background technology
Herbivore Organic substance（volatile organic compounds；VOCs）It is by secondary in plant body
The low boiling of metabolic pathway synthesis, volatile micromolecular compound, content is low, and chemism is high, is weight in ecosystem
The chemical information transmission material wanted, can be by the aerial partss of plant（As, flower, really, leaf）Discharge with Different Organs such as roots and produce.Mesh
Front identify more than 1700 kind of volatile organic matter from kind more than 90 is equal, platymiscium, its constituent mainly include terpenoid,
Alkane, alkene, alcohols, aldehydes, esters, the compound containing carboxyl and carboxyl class etc..Wherein terpene substances are to constitute plant VOCs group
The important component divided.These VOCs are widely present in the air, largely impact air quality and health；In forest
In ecosystem, VOCs is to communicate between development of plants and procreation, mediated plant and plant and resist the biological and abiotic side of body
The important means such as urgent adverse circumstance.
Compared with aerial partss, the volatile content of root system of plant release is less, collects difficult in situ, leads to its content
Slower with the progress of the biophysiology functions such as composition.It can be seen that, how convenient, accurately and timely collect root system volatile matter,
To its qualitative and quantitative study tool significance.
Content of the invention
In view of the deficiencies in the prior art, the technical problem to be solved is to provide one kind for collecting root system in situ
The assay device of volatile matter and method, not only reasonable in design, and also efficiently convenient.
In order to solve above-mentioned technical problem, the technical scheme is that：A kind of for collecting root system volatile matter in situ
Assay device, including the casing in order to plant seedling growth, four side walls of described casing and bottom lock, the top of described casing
Portion is provided with left top cover able to turn on or off and right top cover, is provided with and runs through in order to plant nursery stock between described left top cover and right top cover
Planting groove, the bottom of described casing is through the external admission line of air inlet order, gas flowmeter, air purifier and air pressure
Contracting machine, the top of described casing is through the external outlet pipe of venthole order and collection and confinement of gases bag.
Preferably, described left top cover is equal in magnitude with right top cover.
Preferably, the two side of described left top cover, right top cover and casing is slidably connected or hinged.
Preferably, the intracavity bottom coiling of described casing has subsidiary conduit, and described subsidiary conduit accesses air inlet through air inlet
Pipeline, described subsidiary conduit is disposed with some passages upward.
A kind of test method for collecting root system volatile matter in situ, including above-mentioned any one be used in situ collect root system
The assay device of volatile matter, comprises the steps of：
（1）Take some plant seedlings, take some casings, open the left top cover of each casing and right top cover, at each
The culture matrix of Different Nutrients concentration is placed in casing；
（2）Each casing such as is assigned with the plant seedlings of quantity, closes and disturbs left top cover and right top cover, and the foundation portion of plant seedlings is firm
It is in well the planting groove between left top cover and right top cover, between the foundation portion of planting groove and plant seedlings, leaves space, planting
One layer of Cotton Gossypii is wrapped up with space between the foundation portion of filling planting groove and plant seedlings at the foundation portion of thing seedling；
（3）Collection and confinement of gases bag, outlet pipe order are accessed venthole, by air compressor, gas flowmeter and admission line
It is linked in sequence；
（4）Start air compressor, gas flowmeter reading is adjusted to default throughput, ventilate certain time, drain air inlet
After original air in pipeline, admission line order is accessed air inlet, opens the switch of collection and confinement of gases bag, through the scheduled time,
Treat collection and confinement of gases bag gassy, first close collection and confinement of gases bag switch, turn off air compressor；
（5）Off-test, takes the gas sample in collection and confinement of gases bag to carry out qualitative and quantitative determination.
Preferably, after described left top cover and right top cover are disturbed in conjunction, using preservative film along left top cover and right top cover joint
Line direction is wound around a few.
Preferably, the intracavity bottom coiling of described casing has subsidiary conduit, and described subsidiary conduit accesses air inlet through air inlet
Pipeline, described subsidiary conduit is disposed with some passages upward, a diameter of 1 ~ 2mm of described passage.
Preferably, using gas chromatograph-mass spectrometer (GC-MS)（GC-MS）Detect root system release in each collection and confinement of gases bag
Volatile matter, carries out qualitative and quantitative analysis.
Compared with prior art, the invention has the advantages that：Its overall structure of the present invention is simple, cheap,
Draw materials and easy to make, especially can carry out lossless root system under real cultivation condition, in real time, continuously, accurately collect and analyze
The characteristic index such as the quantity of root system volatile matter, species.
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
Brief description
Fig. 1 is the unitary construction schematic diagram of the embodiment of the present invention.
Fig. 2 is the organigram of the left top cover of the embodiment of the present invention and right top cover.
In figure：1- casing, the left top cover of 11-, the right top cover of 12-, 13- planting groove, 2- air inlet, 3- admission line, 4- gas
Effusion meter, 5- air purifier, 6- air compressor, 7- venthole, 8- outlet pipe, 9- collection and confinement of gases bag, 10- auxiliary tube
Road, 101- passage.
Specific embodiment
It is that the features described above of the present invention and advantage can be become apparent, special embodiment below, and coordinate accompanying drawing, make in detail
Carefully it is described as follows.
As shown in Figure 1 and 2, a kind of assay device for collecting root system volatile matter in situ, including in order to plant seedling growth
Casing 1, four side walls of described casing 1 and bottom lock, the top of described casing 1 be provided with left top cover 11 able to turn on or off with
Right top cover 12, is provided with the planting groove 13 running through in order to plant nursery stock, described casing 1 between described left top cover 11 and right top cover 12
Bottom through the external admission line 3 of air inlet 2 order, gas flowmeter 4, air purifier 5 and air compressor 6, described
The top of casing 1 is through the external outlet pipe 8 of venthole 7 order and collection and confinement of gases bag 9；Preferably, four sides of described casing 1
Wall, bottom and left top cover 11 and right top cover 12 are the thick acrylic material of 5 mm or glass is made, and casing seam crossing is preferably adopted
With hot melting process, the industrial glue of disabling, in case the chemical volatile matter influence research effect of glue release, acrylic or glass are no
Taste, good leak tightness；Preferably, described air purifier 5 adopts activated-charcoal column, and described activated-charcoal column is acrylic or glass material
Cylinder shell, inside fills out granular active carbon, full of whole cylinder, free from foreign meter to ensure air cleaning.
In embodiments of the present invention, described left top cover 11 is equal in magnitude with right top cover 12.
In embodiments of the present invention, described left top cover 11, right top cover 12 and the two side of casing 1 are slidably connected or hinged.
In embodiments of the present invention, the intracavity bottom coiling of described casing 1 has subsidiary conduit 10, described subsidiary conduit 10 warp
Air inlet 2 accesses admission line 3, and described subsidiary conduit 10 is disposed with some passages 101 upward, to ensure by air inlet
The gas of 2 entrance casings 1 can be uniformly distributed, and from the bottom of casing 1, the volatile matter that all root systems discharge is rushed to venthole
7.
In embodiments of the present invention, a kind of test method for collecting root system volatile matter in situ, including above-mentioned any one
Plant the assay device for collecting root system volatile matter in situ, comprise the steps of：
（1）Take some plant seedlings, take some casings 1, open the left top cover 11 of each casing 1 and right top cover 12,
Place the culture matrix of Different Nutrients concentration in each casing 1, described culture matrix is sandy soil and crystal mud with 3:1 volume
Ratio mixing, first cleans sandy soil to water clearly with clear water before mixing, no muddy, air-dries stand-by, crystal mud is soaked in pancebrin respectively
With 2 days in phosphorus deficiency nutritional solution；
（2）Each casing 1 such as is assigned with the plant seedlings of quantity, closes and disturbs left top cover 11 and right top cover 12, plant seedlings stretch into
Culture matrix, the foundation portion of plant seedlings is just at the planting groove 13 between left top cover 11 and right top cover 12, planting groove 13
In the centre of left top cover 11 and right top cover 12 closing line, plant seedlings are also located just at the centre of casing 1, when planting groove 13
Leave space and the foundation portion of plant seedlings between, wrap up one layer of Cotton Gossypii at the foundation portion of plant seedlings with filling planting groove 13
Space and the foundation portion of plant seedlings between, can protect the extruding damage from left top cover 11 and right top cover 12 for the foundation portion of seedling
Wound, also can prevent root system from discharging the loss of volatile matter in certain air pressure；
（3）Collection and confinement of gases bag 9 and outlet pipe 8 order are accessed venthole 7, by air compressor 6 and gas flowmeter 4 order
Access admission line 3；
（4）Start air compressor 6, gas flowmeter 4 reading is adjusted to default throughput, ventilate certain time, preferably 2 points
More than clock, after draining the original air in admission line 3, admission line order 3 is accessed air inlet 2, open collection and confinement of gases bag 9
Switch, through the scheduled time, treat collection and confinement of gases bag 9 gassy, first close collection and confinement of gases bag 9 switch, turn off air compression
Machine 6；
（5）Off-test, takes the gas sample in collection and confinement of gases bag 9 to carry out qualitative and quantitative determination.
In embodiments of the present invention, in order to preferably carry out qualitative and quantitative determination, matched group, casing in matched group are set
On do not cultivate plant seedlings, place and step in casing（1）Identical culture matrix, remaining all parts and operating procedure all with
Above-mentioned consistent.
In embodiments of the present invention, closely water-tight in order to ensure seam, disturb described left top cover 11 and right top cover 12 in conjunction
Afterwards, it is wound around a few using preservative film along the closing line direction of left top cover 11 and right top cover 12.
In embodiments of the present invention, the intracavity bottom coiling of described casing 1 has subsidiary conduit 10, described subsidiary conduit 10 warp
Air inlet 2 accesses admission line 3, and described subsidiary conduit 10 is disposed with some passages 101 upward, described passage 101
A diameter of 1 ~ 2mm.
In embodiments of the present invention, during test, collection and confinement of gases bag 9, casing 1, gas flowmeter 4, air purifier 5 with
And between air compressor 6, connect good, all no gas leakage.
In embodiments of the present invention, using gas chromatograph-mass spectrometer (GC-MS)（GC-MS）Detect each collection and confinement of gases bag 9
The volatile matter of middle root system release, carries out qualitative and quantitative analysis.
The present invention is not limited to above-mentioned preferred forms, and anyone can show that under the enlightenment of the present invention other are each
The assay device for collecting root system volatile matter in situ of the form of kind and method.All according to scope of the present invention patent done equal
Deng change and modification, the covering scope of the present invention all should be belonged to.
Claims (8)
1. a kind of assay device for collecting root system volatile matter in situ it is characterised in that：Including in order to plant seedling growth
Casing, four side walls of described casing and bottom lock, the top of described casing is provided with left top cover able to turn on or off and right top cover,
It is provided with the planting groove running through in order to plant nursery stock, the bottom of described casing is suitable through air inlet between described left top cover and right top cover
The external admission line of sequence, gas flowmeter, air purifier and air compressor, the top of described casing is through venthole order
External outlet pipe and collection and confinement of gases bag.
2. the assay device for collecting root system volatile matter in situ according to claim 1 it is characterised in that：Described left top
Lid is equal in magnitude with right top cover.
3. the assay device for collecting root system volatile matter in situ according to claim 1 it is characterised in that：Described left top
The two side of lid, right top cover and casing is slidably connected or hinged.
4. the assay device for collecting root system volatile matter in situ according to claim 1 it is characterised in that：Described casing
Intracavity bottom coiling have subsidiary conduit, described subsidiary conduit through air inlet access admission line, on described subsidiary conduit arrange
There are some passages upward.
5. a kind of test method for collecting root system volatile matter in situ it is characterised in that：Including as described in claim 1 ~ 4
Any one is used for collecting the assay device of root system volatile matter in situ, comprises the steps of：
（1）Take some plant seedlings, take some casings, open the left top cover of each casing and right top cover, at each
The culture matrix of Different Nutrients concentration is placed in casing；
（2）Each casing such as is assigned with the plant seedlings of quantity, closes and disturbs left top cover and right top cover, and the foundation portion of plant seedlings is firm
It is in well the planting groove between left top cover and right top cover, between the foundation portion of planting groove and plant seedlings, leaves space, planting
One layer of Cotton Gossypii is wrapped up with space between the foundation portion of filling planting groove and plant seedlings at the foundation portion of thing seedling；
（3）Collection and confinement of gases bag, outlet pipe order are accessed venthole, by air compressor, gas flowmeter and admission line
It is linked in sequence；
（4）Start air compressor, gas flowmeter reading is adjusted to default throughput, ventilate certain time, drain air inlet
After original air in pipeline, admission line order is accessed air inlet, opens the switch of collection and confinement of gases bag, through the scheduled time,
Treat collection and confinement of gases bag gassy, first close collection and confinement of gases bag switch, turn off air compressor；
（5）Off-test, takes the gas sample in collection and confinement of gases bag to carry out qualitative and quantitative determination.
6. the test method for collecting root system volatile matter in situ according to claim 5 it is characterised in that：Disturb institute in conjunction
After stating left top cover and right top cover, it is wound around a few using preservative film along the closing line direction of left top cover and right top cover.
7. the test method for collecting root system volatile matter in situ according to claim 5 it is characterised in that：Described casing
Intracavity bottom coiling have subsidiary conduit, described subsidiary conduit through air inlet access admission line, on described subsidiary conduit arrange
There are some passages upward.
8. the test method for collecting root system volatile matter in situ according to claim 5 it is characterised in that：Using gas phase
Chromatograph-mas spectrometer（GC-MS）Detect the volatile matter of root system release in each collection and confinement of gases bag, carry out qualitative and quantitative point
Analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610995674.9A CN106404472A (en) | 2016-11-12 | 2016-11-12 | Experiment device and method for in-situ collection of root system volatiles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610995674.9A CN106404472A (en) | 2016-11-12 | 2016-11-12 | Experiment device and method for in-situ collection of root system volatiles |
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| Publication Number | Publication Date |
|---|---|
| CN106404472A true CN106404472A (en) | 2017-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610995674.9A Pending CN106404472A (en) | 2016-11-12 | 2016-11-12 | Experiment device and method for in-situ collection of root system volatiles |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109883773A (en) * | 2019-03-19 | 2019-06-14 | 海南大学 | It is a kind of original position root system of plant volatile organic matter collect and measuring method |
| CN110726597A (en) * | 2019-11-04 | 2020-01-24 | 南京科略环境科技有限责任公司 | Dynamic sampling method for vegetation source volatile organic compounds |
| CN111624313A (en) * | 2020-06-23 | 2020-09-04 | 东北林业大学 | Research device and method for allelopathy of volatile organic compounds of plant root system |
| CN111929117A (en) * | 2020-09-29 | 2020-11-13 | 中国农业科学院农业环境与可持续发展研究所 | Methane monitoring device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109883773A (en) * | 2019-03-19 | 2019-06-14 | 海南大学 | It is a kind of original position root system of plant volatile organic matter collect and measuring method |
| CN110726597A (en) * | 2019-11-04 | 2020-01-24 | 南京科略环境科技有限责任公司 | Dynamic sampling method for vegetation source volatile organic compounds |
| CN111624313A (en) * | 2020-06-23 | 2020-09-04 | 东北林业大学 | Research device and method for allelopathy of volatile organic compounds of plant root system |
| CN111929117A (en) * | 2020-09-29 | 2020-11-13 | 中国农业科学院农业环境与可持续发展研究所 | Methane monitoring device |
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