CN106386711B - Insect moisture-preserving culture method and device - Google Patents
Insect moisture-preserving culture method and device Download PDFInfo
- Publication number
- CN106386711B CN106386711B CN201610931055.3A CN201610931055A CN106386711B CN 106386711 B CN106386711 B CN 106386711B CN 201610931055 A CN201610931055 A CN 201610931055A CN 106386711 B CN106386711 B CN 106386711B
- Authority
- CN
- China
- Prior art keywords
- dish
- culture
- insect
- cover
- moisturizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000238631 Hexapoda Species 0.000 title claims abstract description 59
- 238000012136 culture method Methods 0.000 title claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000008223 sterile water Substances 0.000 claims description 12
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 9
- 229910052717 sulfur Inorganic materials 0.000 claims description 9
- 239000011593 sulfur Substances 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 8
- 241000282326 Felis catus Species 0.000 claims description 5
- 241000257303 Hymenoptera Species 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 2
- 239000000123 paper Substances 0.000 description 10
- 239000002775 capsule Substances 0.000 description 4
- 239000000428 dust Substances 0.000 description 4
- 241000382353 Pupa Species 0.000 description 3
- 239000011088 parchment paper Substances 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a moisture-preserving insect culture method and a device thereof, wherein the method comprises the following steps: culturing preparation, feeding, culturing and preserving; the insect moisturizing culture device comprises a dish body and a dish cover; the dish cover is installed on the dish body, a plurality of supporting tables are installed in the dish body, a plurality of air guide holes are formed in the end face of the dish cover, a plurality of observation holes are formed in the dish cover, a cover opening is formed in the upper end of each observation hole and is installed on the dish cover, and a dustproof cover is arranged on the cover opening. Supporting the insect in the culture dish through a supporting bench, the insect does not influence its normal culture with water direct contact, adds water and can guarantee that the insect is in moist environment at the bottom of the culture dish, and the observation hole that the dish was covered makes the cultivation process of insect can direct observation, and need not open the culture dish and introduce the article that influence insect culture such as bacterium, this insect culture method and device of moisturizing is effectual, is convenient for a large amount of specimens simultaneous processing.
Description
Technical Field
The invention relates to the technical field of insect culture and preservation, in particular to a moisture-preserving insect culture method and a moisture-preserving insect culture device.
Background
Once insect pupas or cocoons parasitized by natural enemies such as parasitic bees and insect specimens infected by entomogenous fungi leave the native natural environment, the insect pupas or cocoons and the insect specimens cannot normally develop and cannot produce entomogenous fungi spores to be inactivated due to factors such as water evaporation and infectious microbe pollution. However, when natural enemy insects and entomogenous fungi are researched, the specimens need to be collected back to a laboratory for moisturizing culture, so that the biological activity of the research materials can be ensured. At present, the method of adding filter paper in a small finger tube and the method of adding absorbent cotton in a culture dish are generally adopted to solve the problems, but the method has poor moisturizing effect, requires adding water or absorbent cotton to the filter paper every day, is inconvenient to operate, is easy to cause pollution, has poor moisturizing culture effect, can only place one sample in each small paper tube, and is inconvenient to process when a large number of samples are placed.
Disclosure of Invention
In order to solve the technical problems, the invention provides a moisture-preserving insect culture method and a device thereof.
The invention is realized by the following technical scheme:
a moisture-keeping culture method for insects comprises the following steps:
a. preparing culture: preparing a culture dish with the diameter of 50-120mm by adopting glass or plastic, arranging a plurality of support tables with the diameter of 5-15mm in the dish body, arranging a culture table with an arc-shaped groove on each support table, arranging a guide pipe with the diameter of 2-8mm in the middle of each culture table, and covering the dish body with a dish cover;
b. feeding: placing sterile water or sponge filled with sterile water into the dish body, wherein the quantity of the placed sterile water cannot exceed the top end of the support platform;
c. culturing and preserving: placing the sulfur paper after sterilization treatment on a culture table, placing insect larvae, pupae or cocoons which are parasitized by natural enemies such as parasitic bees and the like and insect specimens infected by entomogenous fungi which need to be cultured on the sulfur paper, and covering the sulfur paper with a dish and the dish and storing the sulfur paper at room temperature.
An insect moisturizing culture device comprises a dish body and a dish cover; the dish cover is installed on the dish body, a plurality of supporting tables are installed in the dish body, a plurality of air guide holes are formed in the end face of the dish cover, a plurality of observation holes are formed in the dish cover, a cover opening is formed in the upper end of each observation hole and installed on the dish cover, and a dust cover is arranged on the cover opening.
When the supporting table is a cylinder, the bottom of the dish body is provided with a water trough, and the middle of the dish body is provided with a through hole.
The supporting table can also be arranged as a culture table, the upper end face of the culture table is provided with an arc-shaped groove, the middle part of the culture table is provided with a vertical conduit, and the conduit is communicated with the bottom of the dish body.
The side surface of the culture platform can also be provided with an inclined surface.
The observation hole is in a circular truncated cone shape.
And a fixing ring is arranged on the outer wall of the cover opening.
And an annular groove matched with the fixing ring is formed in the inner wall of the dustproof cover.
The dust cover is made of transparent materials.
The side surface of the dish cover is also provided with a cap extension.
The invention has the beneficial effects that: support the insect in the culture dish through a supporting bench, the insect does not influence its normal culture with water direct contact, adds water and can guarantee that the insect is in moist environment at the bottom of the culture dish, and the observation hole that the dish was covered makes the cultivation process of insect can direct observation, and need not open the culture dish and get into the article that influence the insect culture such as bacterium, and this insect culture method and device of moisturizing is moisturized effectually, is convenient for a large amount of specimens simultaneous processing.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a structural diagram of the support column of the present invention in the form of a cylinder;
FIG. 3 is a structural view of the support column of the present invention as a culture table;
figure 4 is a cross-sectional view of the capsule of the present invention;
FIG. 5 is a schematic view of the capsule construction of the present invention;
FIG. 6 is a block diagram of a capsule embodiment of the present invention;
figure 7 is a cross-sectional view of the capsule of the present invention;
in the figure: 1-dish body, 11-supporting table, 12-water tank, 13-culture table, 14-inclined plane, 141-arc-shaped groove, 142-guide pipe, 2-dish cover, 21-hat extension, 22-air guide hole, 23-cover opening, 231-fixing ring, 24-observation hole, 3-dustproof cover and 31-annular groove.
Detailed Description
The technical solution of the present invention is further described below with reference to the drawings and the embodiments, but the scope of protection claimed is not limited to the description.
Example one
A moisture-keeping culture method for insects comprises the following steps:
a. preparation of culture: preparing a culture dish with the diameter of 50-120mm by adopting glass or plastic, arranging a plurality of support tables with the diameter of 5-15mm in a dish body, arranging a culture table with an arc-shaped groove on each support table, arranging a guide pipe with the diameter of 2-8mm in the middle of each culture table, and covering the dish body with a dish cover;
b. feeding: placing sterile water or sponge filled with sterile water into the dish body, wherein the quantity of the placed sterile water cannot exceed the top end of the support platform;
c. culturing and preserving: placing sterilized sulfur paper on a culture table, placing insect larvae, pupae or cocoons which are parasitized by natural enemies such as parasitized bees and the like and insect specimens infected by entomogenous fungi to be cultured on the sulfur paper, and covering the sulfur paper with a dish and a cover to store at room temperature.
The insect moisturizing culture device comprises a dish body 1 and a dish cover 2; the dish cover 2 is installed on the dish body 1, a plurality of supporting tables 11 are installed in the dish body 1, a plurality of air guide holes 22 are formed in the end face of the dish cover 2, a plurality of observation holes 24 are formed in the dish cover 2, a cover opening 23 is formed in the upper end of each observation hole 24 and installed on the dish cover 2, and a dust cover 3 is arranged on the cover opening 23. Support the insect in the culture dish through brace table 11, the insect does not influence its normal culture with water direct contact, adds water and can guarantee that the insect is in moist environment at the bottom of the culture dish, and the observation hole 24 on the dish lid 2 makes the cultivation process of insect can direct observation, and need not open the culture dish and introduce article that influence insect culture such as bacterium.
In order to circulate air in the culture dish and the outside and prevent excessive moisture from being lost, when the support table 11 is a cylinder, the bottom of the dish body 1 is provided with a water tank 12, and the middle of the dish body 1 is provided with a through hole.
The support platform 11 can also be set as a culture platform 13, an arc-shaped groove 141 is arranged on the upper end face of the culture platform 13, a vertical conduit 142 is arranged in the middle of the culture platform 13, and the conduit 142 penetrates through the bottom of the dish body 1. The arc-shaped groove 141 can concentrate water in the culture platform 13 at the lowest part of the arc-shaped groove 13 and then flow out of the culture dish from the guide pipe 142, so that the influence on the culture of insects caused by the concentration of water in the culture dish on the culture platform 13 due to long-term accumulation is avoided.
The side surface of the culture platform 13 can also be provided with a slant surface 14.
The observation hole 24 is of a truncated cone shape in order to allow the observer to view a larger area of the inside of the culture dish after passing through the lid 23 and the observation hole 24.
The shield can be for the plain lens design, also can for cat eye design [ the cat eye is formed by a concave lens and a convex lens, and objective is concave lens, and the eyepiece is convex lens, but the focus of objective is shorter, and the focal length of eyepiece is longer, and the focal length of eyepiece should be equal to or be greater than the distance (the length of cat eye) of objective and eyepiece and the focal length sum of eyepiece ].
In order to fix the dust-proof cover 3 conveniently, a fixing ring 231 is arranged on the outer wall of the cover opening 23.
And an annular groove 31 matched with the fixing ring 231 is formed in the inner wall of the dustproof cover 3.
The dust cover 3 is made of transparent materials.
The side surface of the dish cover 2 is also provided with a cap extension 21. The cap delay 21 enables the dish cover 2 to have a larger force application point when being opened, so that the dish cover is convenient to open.
When the support base 11 is cylindrical as shown in FIG. 2, it is suitable for mass cultivation; add sterile water or inhale the sponge that is full of sterile water in basin 12 at the bottom of the culture dish earlier, the water yield is no longer than the cylinder height, puts the parchment paper after the sterilization on the cylinder, puts into the similar sample of the insect larva, pupa or cocoon or by worm fungus infection of cultivateing on the parchment paper, then covers dish lid 2, can observe the growth condition of insect through observation hole 24 at the culture in-process.
As shown in the fig. 3 and 4, when the support platform 11 is the culture platform 13, firstly, sterile water or sponge filled with sterile water is added into the culture dish bottom 2, the water volume and the height of the sponge are not more than the height of the cylinder, then the same kind/type of the cultured insect larvae, pupae or cocoons or insect specimens infected by insect fungi are put into the culture platform 13, then the dish cover 2 is covered, or the cultured insect specimens can be put into the culture platform 13 after the parchment paper is put once, and the growth condition of the insect/entomogenous fungi can be observed through the observation hole 24 in the culture process.
As shown in FIG. 6, in order to better observe the growth of the insects, a plurality of observation holes 24 are formed in the dish cover 2, and when the support platform 11 is the culture platform 13, the observation holes 24 can be arranged right above the culture platform 13, so that the growth of the insects can be observed more intuitively.
Claims (8)
1. An insect moisturizing culture method is characterized in that: the method comprises the following steps:
a. preparing culture: preparing a culture dish with the diameter of 50-120mm by adopting glass or plastic, arranging a plurality of support tables (11) with the diameter of 5-15mm in the middle of the dish body (1), arranging a culture table (13) with an arc-shaped groove (141) on each support table (11), arranging a guide pipe (142) with the diameter of 2-8mm in the middle of each culture table (13), and covering the dish body (1) with a dish cover (2);
b. feeding: putting sterile water or sponge filled with the sterile water into the dish body (1), wherein the amount of the put water cannot exceed the top end of the support table (11);
c. and (3) culture and preservation: placing the sterilized sulfur paper on a culture table (13), placing insect larvae, pupae or cocoons which are parasitized by natural enemies such as parasitized bees and the like and insect specimens infected by entomogenous fungi to be cultured on the sulfur paper, and covering a dish cover (2) to store at room temperature;
the insect moisturizing culture device used in the method comprises a dish body (1) and a dish cover (2), wherein the dish cover (2) is installed on the dish body (1), a plurality of supporting tables (11) are installed in the dish body (1), a plurality of air guide holes (22) are formed in the end face of the dish cover (2), a plurality of observation holes (24) are formed in the dish cover (2), a cover opening (23) is formed in the upper end of each observation hole (24) and is installed on the dish cover (2), and a dustproof cover (3) is arranged on the cover opening (23);
the observation hole (24) is in a circular truncated cone shape;
the dustproof cover is designed to be a cat eye, the cat eye consists of a concave lens and a convex lens, the objective lens is a concave lens, and the ocular lens is a convex lens.
2. The insect moisturizing culture method of claim 1, wherein: when the supporting table (11) is cylindrical, a water trough (12) is arranged at the bottom of the dish body (1), and a through hole is formed in the middle of the dish body (1).
3. The insect moisturizing culture method of claim 1, wherein: the support table (11) can also be set as a culture table (13), an arc-shaped groove (141) is formed in the upper end face of the culture table (13), a vertical guide pipe (142) is arranged in the middle of the culture table (13), and the guide pipe (142) penetrates through the bottom of the dish body (1).
4. The insect moisturizing culture method of claim 1, wherein: the side surface of the culture platform (13) can also be provided with an inclined surface (14).
5. The insect moisturizing culture method of claim 1, wherein: and a fixing ring (231) is arranged on the outer wall of the cover opening (23).
6. The insect moisturizing culture method of claim 5, wherein: be provided with annular groove (31) that matches with retainer plate (231) on shield (3) inner wall.
7. The insect moisturizing culture method of claim 1, wherein: the dustproof cover (3) is made of transparent materials.
8. The insect moisturizing culture method of claim 1, wherein: the side surface of the dish cover (2) is also provided with a cap extension (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931055.3A CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931055.3A CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106386711A CN106386711A (en) | 2017-02-15 |
| CN106386711B true CN106386711B (en) | 2022-07-26 |
Family
ID=58012913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610931055.3A Active CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106386711B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107258150A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture dish |
| CN107258151A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture apparatus |
| WO2019178791A1 (en) * | 2018-03-21 | 2019-09-26 | 苏州大学张家港工业技术研究院 | Bombyx mori hatching and young silkworm rearing frame |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640730A (en) * | 2012-04-13 | 2012-08-22 | 中国科学院东北地理与农业生态研究所 | In vivo reproduction method of entomopathogenic nematodes |
| KR20120126320A (en) * | 2011-05-11 | 2012-11-21 | 김종택 | A Box for farming a snail |
| KR20130005856U (en) * | 2012-03-29 | 2013-10-10 | 김유신 | Earthworms are landfill in pots of food boxes |
| CN204579580U (en) * | 2015-05-04 | 2015-08-26 | 中国农业科学院草原研究所 | Larva raising feeding device |
| CN205093396U (en) * | 2015-11-02 | 2016-03-23 | 金海韵 | Box is raised to snail |
| CN205124775U (en) * | 2015-12-03 | 2016-04-06 | 长江大学 | Apparatus is trained in small insects's cold -resistant experiment |
| CN205320955U (en) * | 2016-01-29 | 2016-06-22 | 华中农业大学 | Phytopathogenic nematode incubator |
| CN206390058U (en) * | 2016-10-31 | 2017-08-11 | 遵义市林业科学研究所 | A kind of insect moisturizing culture apparatus |
-
2016
- 2016-10-31 CN CN201610931055.3A patent/CN106386711B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120126320A (en) * | 2011-05-11 | 2012-11-21 | 김종택 | A Box for farming a snail |
| KR20130005856U (en) * | 2012-03-29 | 2013-10-10 | 김유신 | Earthworms are landfill in pots of food boxes |
| CN102640730A (en) * | 2012-04-13 | 2012-08-22 | 中国科学院东北地理与农业生态研究所 | In vivo reproduction method of entomopathogenic nematodes |
| CN204579580U (en) * | 2015-05-04 | 2015-08-26 | 中国农业科学院草原研究所 | Larva raising feeding device |
| CN205093396U (en) * | 2015-11-02 | 2016-03-23 | 金海韵 | Box is raised to snail |
| CN205124775U (en) * | 2015-12-03 | 2016-04-06 | 长江大学 | Apparatus is trained in small insects's cold -resistant experiment |
| CN205320955U (en) * | 2016-01-29 | 2016-06-22 | 华中农业大学 | Phytopathogenic nematode incubator |
| CN206390058U (en) * | 2016-10-31 | 2017-08-11 | 遵义市林业科学研究所 | A kind of insect moisturizing culture apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106386711A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106386711B (en) | Insect moisture-preserving culture method and device | |
| Choi et al. | Single spore isolation of fungi | |
| Wood | Nematode feeding relationships: Feeding relationships of soil-dwelling nematodes | |
| CN203467354U (en) | Water-planting device for tissue culture seedlings | |
| CN102972446A (en) | Preparation method of steinernema carpocapsae | |
| CN109082366A (en) | A kind of device and method separating Pyricularia oryzae single-ascospore strain | |
| CN105296386B (en) | The interior raw bacillus amyloliquefaciens of one plant height production antagonism tobacco bacterial wilt active material | |
| CN109355208A (en) | A kind of highly pathogenicity biocontrol microorganisms Java cordyceps sinensis and its application | |
| CN103820381B (en) | A kind of primary culture method of striped rice borer cell | |
| CN103918611A (en) | Simple wheat aphid species assay method | |
| CN206390058U (en) | A kind of insect moisturizing culture apparatus | |
| CN207355280U (en) | A kind of breeding apparatus of indoor feeding planthopper | |
| CN205623962U (en) | Insect rearing cage | |
| CN106065392A (en) | A kind of diaphorina citri highly pathogenicity fumosorosea bacterial strain and application thereof | |
| Linford | Methods of observing soil flora and fauna associated with roots | |
| CN104004663A (en) | Preparation method of Duddingtonia flagrans biological freeze-drying preparation | |
| CN203613177U (en) | Culture device applicable to microorganism separation and purification | |
| CN110373331A (en) | The Huperzia serrata endogenetic epiphyte of one plant of anti-botrytis cinerea and its application | |
| CN205695089U (en) | Insect observation device | |
| CN113455468B (en) | Industrialized breeding method of sterile cordyceps sinensis host larvae | |
| CN107549121A (en) | The application of the method for breeding of indoor feeding planthopper, breeding apparatus and breeding apparatus | |
| RU182023U1 (en) | GROWTH CAMERA FOR CULTIVATION OF MICRO-ORGANISMS | |
| CN210039372U (en) | Lotus grass direct chest flea beetle experiment observation tube | |
| CN208000305U (en) | A kind of Similis Contact toxicity measurement device | |
| CN218635098U (en) | A device that is used for function plant to raise natural enemy and investigate parasitic rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |