CN106386711A - Insect moisture-preserving culture method and device thereof - Google Patents
Insect moisture-preserving culture method and device thereof Download PDFInfo
- Publication number
- CN106386711A CN106386711A CN201610931055.3A CN201610931055A CN106386711A CN 106386711 A CN106386711 A CN 106386711A CN 201610931055 A CN201610931055 A CN 201610931055A CN 106386711 A CN106386711 A CN 106386711A
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- CN
- China
- Prior art keywords
- insect
- culture
- ware
- lid
- moisturizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000238631 Hexapoda Species 0.000 title claims abstract description 53
- 238000012136 culture method Methods 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 17
- 230000003020 moisturizing effect Effects 0.000 claims description 21
- 244000144985 peep Species 0.000 claims description 16
- 239000000428 dust Substances 0.000 claims description 12
- 241000233866 Fungi Species 0.000 claims description 7
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 7
- 239000005864 Sulphur Substances 0.000 claims description 7
- 241000382353 Pupa Species 0.000 claims description 6
- 241001414987 Strepsiptera Species 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 241001481304 Vespoidea Species 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 230000009545 invasion Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 description 4
- 239000011022 opal Substances 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 206010017533 Fungal infection Diseases 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 210000004932 little finger Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an insect moisture-preserving culture method and device thereof. The insect moisture-preserving culture method comprises the steps of culture preparation, material feeding and culture and preservation. The insect moisture-preserving culture device comprises a vessel body and a vessel cover, wherein the vessel cover is installed on the vessel body, a plurality of supporting tables are installed in the vessel body, a plurality of air guiding holes are formed in the end face of the vessel cover, a plurality of observing holes are formed in the vessel cover, and cap openings formed in the upper end of the observing holes are installed on the vessel cover and are provided with dust-proof caps. An insect is supported in the culture vessel through the supporting tables and is not in direct contact with water, accordingly normal culture of the insect is not influenced, water is added to the bottom of the culture vessel so that it can be ensured that the insect is in a humid environment, and the observing holes formed in the vessel cover make the insect culture process observed directly without vessel opening causing invasion of bacteria and other articles affecting insect culture. The insect moisture-preserving culture method and the insect moisture-preserving culture device have a good moisture-preserving culture effect and facilitate simultaneous processing of a large number of specimens.
Description
Technical field
The present invention relates to insect culture Techniques of preserving field is and in particular to a kind of insect moisturizing cultural method and its device.
Background technology
The insect specimen infected by the parasitic insect pupa of the natural enemies such as parasitic wasp or cocoon and by entomogenous fungi once leaves it
Primary natural environment, can be because the factors such as moisture evaporation, living contaminants lead to not normal development and cannot produce entomogenous fungi spore
Son and inactivate.But studying natural enemy insect and during entomogenous fungi, need for these collections of specimens to go back to laboratory and carry out moisturizing training
Support, the biologically active of guarantee research material.At present, the approach solving the above problems, generally adopts little finger of toe Guan Zhongjia filter paper
Method and culture dish in plus absorbent cotton method, but said method moistening effect is poor, need add water to filter paper daily or add
Absorbent cotton, operation inconvenience, and easily cause pollution, moisturizing culture effect is poor, and can only place a sample in each conette,
It is not easy to process during a large amount of sample.
Content of the invention
For solving above-mentioned technical problem, the invention provides a kind of insect moisturizing cultural method and its device.
The present invention is achieved by the following technical programs：
A kind of insect moisturizing cultural method, the method comprises the following steps：
A, culture prepare：It is prepared into the culture dish of a diameter of 50-120mm using glass or plastics, in the middle setting of ware body
The supporting table of several a diameter of 5-15mm, setting in supporting table has arc tank culture platform, and the middle part of culture platform has directly
Footpath is 2-8mm conduit, and ware lid covers on ware body；
B, pan feeding：By sterilized water or the sponge that is filled sterilized water is put in ware body, the water yield put into not can exceed that support
Platform top；
C, culture preserve：Sulphur paper through sterilization treatment is placed on culture platform, then by needs culture by parasitic wasp
The insect specimen infected Deng the parasitic insect larvae of natural enemy, pupa or cocoon and by entomogenous fungi is placed on sulphur paper, covers ware lid
Put and preserve at room temperature.
A kind of insect moisturizing culture apparatus, including ware body, ware lid；Described ware lid is arranged on ware body, peace in described ware body
Equipped with some supporting tables, described ware lid end face has some gas ports, ware lid is provided with some peep holes, on peep hole
End has lid mouth to be arranged on ware lid, and described lid mouth is provided with dust cap.
Described supporting table be cylinder when, ware body bottom be provided with tank, the middle part of ware body is provided with through hole.
Described supporting table may be arranged as cultivating platform, and the upper surface of culture platform is provided with deep-slotted chip breaker, the middle part of culture platform
Have vertical conduit, conduit insertion is in the bottom of ware body.
The side of described culture platform may be arranged as inclined-plane.
Described peep hole is circular platform type.
It is provided with retainer plate on described lid mouth outer wall.
It is provided with, on described dust cap inwall, the annular groove mating with retainer plate.
Described dust cap is transparent material.
The side of described ware lid is additionally provided with cap and prolongs.
The beneficial effects of the present invention is：By supporting table, insect is supported that insect is not directly connect with water in culture dish
Touch and affect it and normally cultivate, add water to ensure that the observation in the environment that insect is in humidity, on ware lid at culture dish bottom
Hole enables the incubation of insect directly to observe, and entering bacterium etc. without opening culture dish affects insect culture
Article, this insect moisturizing cultural method and device moisturizing culture effect are good, are easy to a large amount of samples and process simultaneously.
Brief description
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structure chart that the new support column of this practicality is during cylinder；
Fig. 3 is the structure chart that support column of the present invention is culture platform；
Fig. 4 is the sectional view of ware body of the present invention；
Fig. 5 is the ware lid structural representation of the present invention；
Fig. 6 is the ware lid example structure figure of the present invention；
Fig. 7 is the ware lid sectional view of the present invention；
In figure：1- ware body, 11- supporting table, 12- tank, 13- culture platform, 14- inclined-plane, 141- deep-slotted chip breaker, 142- conduit,
2- ware lid, 21- cap prolongs, 22- gas port, 23- lid mouth, 231- retainer plate, 24- peep hole, 3- dust cap, 31- annular groove.
Specific embodiment
Below in conjunction with drawings and Examples, technical scheme is further described, but claimed scope is simultaneously
It is not limited to described.
Embodiment one
A kind of insect moisturizing cultural method, the method comprises the following steps：
A, culture prepare：It is prepared into the culture dish of a diameter of 50-120mm using glass or plastics, in the middle setting of ware body
The supporting table of several a diameter of 5-15mm, setting in supporting table has arc tank culture platform, and the middle part of culture platform has directly
Footpath is 2-8mm conduit, and ware lid covers on ware body；
B, pan feeding：By sterilized water or the sponge that is filled sterilized water is put in ware body, the water yield put into not can exceed that support
Platform top；
C, culture preserve：Sulphur paper through sterilization treatment is placed on culture platform, then by needs culture by parasitic wasp
The insect specimen infected Deng the parasitic insect larvae of natural enemy, pupa or cocoon and by entomogenous fungi is placed on sulphur paper, covers ware lid
Put and preserve at room temperature.
A kind of insect moisturizing culture apparatus as depicted, including ware body 1, ware lid 2；Described ware lid 2 is arranged on ware body 1
On, some supporting tables 11 are installed in described ware body 1, described ware lid 2 end face has some gas ports 22, ware lid 2 is arranged
There are some peep holes 24, have lid mouth 23 to be arranged on ware lid 2 in peep hole 24 upper end, described lid mouth 23 is provided with dust cap 3.
By supporting table 11 by insect support in culture dish, insect not with water directly contact and affect it and normally cultivate, in culture dish
Bottom adds water to ensure that in the environment that insect is in humidity, the peep hole 24 on ware lid 2 enables the incubation of insect straight
Connect observation, introducing bacterium etc. without opening culture dish affects the article of insect culture.
For air and extraneous circulation in culture dish, also it is unlikely to the excessive moisture that scatters and disappears, described supporting table 11 is cylinder
When, ware body 1 bottom be provided with tank 12, the middle part of ware body 1 is provided with through hole.
Described supporting table 11 may be arranged as cultivating platform 13, and the upper surface of culture platform 13 is provided with deep-slotted chip breaker 141, culture
The middle part of platform 13 has vertical conduit 142, and conduit 142 insertion is in the bottom of ware body 1.Deep-slotted chip breaker 141 can be by culture platform 13
Interior water concentrates on deep-slotted chip breaker 13 lowest part and then flows out culture dish in conduit 142, it is to avoid the moisture in culture dish is long-term
Accumulate and concentrate on and on culture platform 13, the culture of insect is impacted.
The side of described culture platform 13 may be arranged as inclined-plane 14.
In order that observer sight line can watch within culture dish more through after lid mouth 23 and peep hole 24
Large area, described peep hole 24 is circular platform type.
Described dust cap is can to design for flat mirror or opal design【" opal " is by concavees lens and one
Individual convex lens composition, object lens are concavees lens, and eyepiece is convex lens, but the focal length of object lens is shorter, and the defocus distance of eyepiece is longer, and mesh
The focal length of mirror should be equal to or more than object lens and the distance (length of opal) of eyepiece and the focal length sum of eyepiece】.
The fixation of dust cap 3 for convenience, described lid mouth 23 outer wall is provided with retainer plate 231.
It is provided with, on described dust cap 3 inwall, the annular groove 31 mating with retainer plate 231.
Described dust cap 3 is transparent material.
The side of described ware lid 2 is additionally provided with cap and prolongs 21.Cap prolongs 21 makes ware lid 2 have larger impetus when opening,
Ware lid is made to open convenient.
As shown in Figure 2 when supporting table 11 is for cylinder, it is suitable for large batch of culture；The first tank 12 at culture dish bottom
The interior sponge adding sterilized water or being filled sterilized water, the water yield is less than the height of cylinder, the sulphur after putting sterilizing on cylinder
Sour paper, puts into insect larvae, pupa or cocoon or the similar sample by the insect specimen of worm fungal infection of culture, so on template
Afterwards ware lid 2 is covered, incubation can be observed by peep hole 24 the growth situation of insect.
When supporting table 11 is culture platform 13, in the 2 of culture dish bottom, first adds sterilized water or be filled as in Fig. 3,4
The height of the sponge of sterilized water, the water yield and sponge is less than the height of cylinder, then puts into the elder brother of culture on individual culture platform 13
Then ware lid 2 is covered it is also possible to train by worm larva, pupa or cocoon or the of the same race/class sample by the insect specimen of worm fungal infection
Put the insect specimen of culture after template is put on foster platform 13 again, elder brother can be observed by peep hole 24 in incubation
The growth situation of worm/entomogenous fungi.
As shown in Figure 6, in order to preferably observe the growing state of insect, some peep holes 24 are opened on ware lid 2, when
When support platform 11 is culture platform 13, peep hole 24 can be arranged on the surface of culture platform 13, more intuitively observes insect
Growing state.
Claims (10)
1. a kind of insect moisturizing cultural method it is characterised in that：The method comprises the following steps：
A, culture prepare：It is prepared into the culture dish of a diameter of 50-120mm using glass or plastics, some in the middle setting of ware body
The supporting table of individual a diameter of 5-15mm, setting in supporting table has an arc tank culture platform, cultivate platform middle part have a diameter of
2-8mm conduit, ware lid covers on ware body；
B, pan feeding：By sterilized water or the sponge that is filled sterilized water is put in ware body, the water yield put into not can exceed that supporting table top
End；
C, culture preserve：Sulphur paper through sterilization treatment is placed on culture platform, then by needs culture by skies such as parasitic wasps
The parasitic insect larvae of enemy, pupa or cocoon and the insect specimen being infected by entomogenous fungi are placed on sulphur paper, cover ware lid and are placed on
Preserve under room temperature.
2. a kind of insect moisturizing culture apparatus, including ware body (1), ware lid (2) it is characterised in that：Described ware lid (2) is arranged on ware
On body (1), some supporting tables (11) are installed in described ware body (1), described ware lid (2) end face has some gas ports
(22), ware lid (2) is provided with some peep holes (24), has lid mouth (23) to be arranged on ware lid (2) in peep hole (24) upper end,
It is provided with dust cap (3) on described lid mouth (23).
3. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：When described supporting table (11) is cylinder, ware
Body (1) bottom be provided with tank (12), the middle part of ware body (1) is provided with through hole.
4. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：Described supporting table (11) may be arranged as
Culture platform (13), the upper surface of culture platform (13) is provided with deep-slotted chip breaker (141), and the middle part of culture platform (13) has vertical conduit
(142), conduit (142) insertion is in the bottom of ware body (1).
5. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：The side of described culture platform (13) is acceptable
It is set to inclined-plane (14).
6. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：Described peep hole (24) is circular platform type.
7. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：It is provided with solid on described lid mouth (23) outer wall
Fixed circle (231).
8. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：It is provided with described dust cap (3) inwall
The annular groove (31) mating with retainer plate (231).
9. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：Described dust cap (3) is transparent material.
10. insect moisturizing culture apparatus as claimed in claim 2 it is characterised in that：The side of described ware lid (2) is additionally provided with
Cap prolongs (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931055.3A CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610931055.3A CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106386711A true CN106386711A (en) | 2017-02-15 |
| CN106386711B CN106386711B (en) | 2022-07-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610931055.3A Active CN106386711B (en) | 2016-10-31 | 2016-10-31 | Insect moisture-preserving culture method and device |
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| Country | Link |
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| CN (1) | CN106386711B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107258151A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture apparatus |
| CN107258150A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture dish |
| US20210000093A1 (en) * | 2018-03-21 | 2021-01-07 | Soochow University | Bombyx mori hatching and young silkworm rearing frame |
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| KR20120126320A (en) * | 2011-05-11 | 2012-11-21 | 김종택 | A Box for farming a snail |
| KR20130005856U (en) * | 2012-03-29 | 2013-10-10 | 김유신 | Earthworms are landfill in pots of food boxes |
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| CN205093396U (en) * | 2015-11-02 | 2016-03-23 | 金海韵 | Box is raised to snail |
| CN205124775U (en) * | 2015-12-03 | 2016-04-06 | 长江大学 | Apparatus is trained in small insects's cold -resistant experiment |
| CN205320955U (en) * | 2016-01-29 | 2016-06-22 | 华中农业大学 | Phytopathogenic nematode incubator |
| CN206390058U (en) * | 2016-10-31 | 2017-08-11 | 遵义市林业科学研究所 | A kind of insect moisturizing culture apparatus |
-
2016
- 2016-10-31 CN CN201610931055.3A patent/CN106386711B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120126320A (en) * | 2011-05-11 | 2012-11-21 | 김종택 | A Box for farming a snail |
| KR20130005856U (en) * | 2012-03-29 | 2013-10-10 | 김유신 | Earthworms are landfill in pots of food boxes |
| CN102640730A (en) * | 2012-04-13 | 2012-08-22 | 中国科学院东北地理与农业生态研究所 | In vivo reproduction method of entomopathogenic nematodes |
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| CN205124775U (en) * | 2015-12-03 | 2016-04-06 | 长江大学 | Apparatus is trained in small insects's cold -resistant experiment |
| CN205320955U (en) * | 2016-01-29 | 2016-06-22 | 华中农业大学 | Phytopathogenic nematode incubator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107258151A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture apparatus |
| CN107258150A (en) * | 2017-07-14 | 2017-10-20 | 广西壮族自治区农业科学院经济作物研究所 | One cultivates peanut rudiment culture dish |
| US20210000093A1 (en) * | 2018-03-21 | 2021-01-07 | Soochow University | Bombyx mori hatching and young silkworm rearing frame |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106386711B (en) | 2022-07-26 |
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