CN106386595A - Microelement nutrition reinforcing method capable of promoting development of larval of Macrobrachium nipponense - Google Patents
Microelement nutrition reinforcing method capable of promoting development of larval of Macrobrachium nipponense Download PDFInfo
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- CN106386595A CN106386595A CN201610798468.9A CN201610798468A CN106386595A CN 106386595 A CN106386595 A CN 106386595A CN 201610798468 A CN201610798468 A CN 201610798468A CN 106386595 A CN106386595 A CN 106386595A
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- artemia
- paedomorphosis
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- macrobrachium nipponensis
- fish oil
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- 241000058338 Macrobrachium nipponense Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 39
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 26
- 230000035764 nutrition Effects 0.000 title claims abstract description 26
- 230000001737 promoting effect Effects 0.000 title claims abstract description 16
- 230000001418 larval effect Effects 0.000 title abstract 7
- 230000003014 reinforcing effect Effects 0.000 title abstract 3
- 241001247197 Cephalocarida Species 0.000 claims abstract description 51
- 239000011669 selenium Substances 0.000 claims abstract description 28
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 27
- 235000021323 fish oil Nutrition 0.000 claims abstract description 27
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 27
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 25
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 23
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052802 copper Inorganic materials 0.000 claims abstract description 18
- 239000010949 copper Substances 0.000 claims abstract description 18
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000011701 zinc Substances 0.000 claims abstract description 16
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 16
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 11
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 11
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 9
- 238000005728 strengthening Methods 0.000 claims description 30
- 239000011573 trace mineral Substances 0.000 claims description 22
- 235000013619 trace mineral Nutrition 0.000 claims description 22
- 230000001804 emulsifying effect Effects 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000012423 maintenance Methods 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 229910052738 indium Inorganic materials 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 230000002787 reinforcement Effects 0.000 abstract description 3
- 239000000969 carrier Substances 0.000 abstract 1
- 229910000365 copper sulfate Inorganic materials 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 239000011785 micronutrient Substances 0.000 description 4
- 235000013369 micronutrients Nutrition 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 210000000481 breast Anatomy 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 241000238582 Artemia Species 0.000 description 2
- 241000238017 Astacoidea Species 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a microelement nutrition reinforcing method capable of promoting development of larval of Macrobrachium nipponense. The method includes: using high concentrated chlorella as microelement enriched carriers, and preparing a certain concentration of a copper sulfate solution and a zinc sulfate solution; using the solutions to perform enrichment of microelement copper and zinc on the chlorella, putting the chlorella into a water tank with artemia after enrichment is completed, and adding emulsified fish oil and selenium yeast into the water tank to reinforce the artemia; and finally feeding the larval of the Macrobrachium nipponense with the reinforced artemia. The microelement nutrition reinforcing method capable of promoting development of the larval of the Macrobrachium nipponense can perform microelement reinforcement on the artemia for the the larval of the Macrobrachium nipponense, and can feed the larval of the Macrobrachium nipponense with the microelement reinforced artemia to significantly improve the survival rate of the larval.
Description
Technical field
The present invention relates to a kind of fortification method, more particularly to a kind of micro unit promoting Macrobrachium nipponensis paedomorphosis
The method of element strengthening artemia.
Background technology
The artificial breeding Macrobrachium nipponensis young needs substantial amounts of living body bait, and the artemia of artificial culture is generally as Shrimp waste children
The living body bait of body.During propagating artificially, the nutrient balance of the open-mouthed bait of stage of development rises to the seed yield in later stage
To key effect.The micro- prothetic group as some enzymes and metal-binding sites, are able to maintain that skeleton development, promote body to exempt from
Epidemic disease function is normally carried out with holding metabolism.This laboratory research shows, copper, zinc and Se content in the artemia of artificial culture
Less than the requirement that can not meet Macrobrachium nipponensis paedomorphosis.Compared to natural water area, the Macrobrachium nipponensis young is propagating ring artificially
The living body bait limitednumber ingested under border, practice produces and generally feeds to after living body bait wheel animalcule fortification, but often neglects
Omit the fortification of trace element.
At present the method for fortification artemia has multiple, and its cost and effect are also had nothing in common with each other, to artemia feed Micro Algae,
Yeast and fish oil are relatively conventional schedule of reinforcements, and existing intensifying method is limited only to supplement fat in living body bait wheel animalcule
Fat acid and vitamin, often have ignored the supplement of external source trace element.Copper, zinc and selenium are all water-soluble substanceses, and artemia can not
Absorb trace element in water body.So far, the method for aliphatic acid and vitamin enrichment artemia can be not applied directly to trace quantity mineral unit
Element, and the strengthening artemia method of water soluble trace elements is not yet set up.So, find artemia trace element reinforcement technique, with full
Sufficient Macrobrachium nipponensis paedomorphosis demand, becomes the problem of urgent need to resolve.
Content of the invention
It is an object of the invention in order to overcome above the deficiencies in the prior art to provide a kind of promotion Macrobrachium nipponensis young
The micro-element nutrition intensifying method developed, carrys out Rich in Trace Element by using highly enriched chlorella, micro- by using being enriched with
Chlorella after secondary element strengthens artemia further to artemia feeding, utilizes emulsifying fish oil and yeast selenium directly to artemia simultaneously
Strengthened so that artemia has reached the full nutritional status required for Macrobrachium nipponensis paedomorphosis, being capable of high degree lifting day
The growing of this pond crayfish young.
Technical scheme is as follows：
A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis, concrete grammar comprises the following steps：
Step one, is Rich in Trace Element carrier using high enrichment chlorella；
Step 2, compound concentration is 500-1000 mg L−1Copper-bath and concentration be 200-500 mg L-1Zinc sulfate
Solution；
Step 3, takes the high enrichment chlorella of step one to add the copper-bath for preparing of step 2 and solution of zinc sulfate
Carry out Rich in Trace Element copper and zinc in mixed solution；
Step 4, the chlorella after step 2 Rich in Trace Element copper and zinc is taken out；Put in the tank that cultivation has artemia, with
When add emulsifying fish oil and yeast selenium that artemia is strengthened；
Step 5, carries out feeding using the artemia after strengthening to the Macrobrachium nipponensis young.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, joins in step one
The weight in wet base putting chlorella is 7 × 1010cells g−1.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, will in step 3
10g chlorella is added in 3-5ml copper-bath and the mixed liquor of 3-5ml solution of zinc sulfate.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, rich in step 3
The enrichment time integrating trace copper and zinc is as 1-3h.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis is strong in step 4
Change temperature is 28 ± 0.5 DEG C, pH 7.8-8.2, dissolved oxygen are more than 6 mg/L, salinity 20 ‰.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, breast in step 4
The addition of change type fish oil is 2mg/L.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, yeast selenium in step 4
Addition be 2mg/L.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, when strengthening in step 4
Between be 8-12h.
Further, the micro-element nutrition intensifying method of described promotion Macrobrachium nipponensis paedomorphosis, adds breast in step 4
The process that change type fish oil and yeast selenium are strengthened to artemia is carried out in two stages, and the first stage is will to be passed through oxygen in tank
And system temperature is warming up to 30-32 DEG C, keep 1-2h, be subsequently adding emulsifying fish oil, add emulsifying fish oil to support with tank
The mass volume ratio growing liquid is 1mg/L, keeps 1-2h, then adds yeast selenium, keeps 1-2h, adds amount and the breast of yeast selenium
The amount of change type fish oil is identical；Then system temperature is reduced to 15-18 DEG C, keeps 8-10h, enter second stage, keep system
Temperature adds emulsifying fish oil and yeast selenium, the emulsifying fish oil of addition and the amount of yeast selenium and water under conditions of being 15-18 DEG C
The mass volume ratio that groove cultivates liquid system is 1mg/L, and after addition, maintenance system dissolved oxygen amount is more than 6mg/L, pH7.8-8.2, salt
Spend 20 ‰, in 3-4h, tank culturing liquid system temperature is risen to 30 DEG C, keep 1-2h, strengthening completes.
The present invention passes through the enrichment using chlorella to the trace element such as copper and zinc, using the chlorella after enrichment to artemia
Carry out feeding strengthening, greatly improve the content of trace element in artemia body, entered by the way of strengthening artemia stage by stage simultaneously
One step improves the strengthening effect of artemia nutrient, carries out feeding using the artemia after strengthening to the Macrobrachium nipponensis young, can
It is obviously improved the survival rate of the pond crayfish young.
Brief description
Fig. 1 is the artemia micronutrient levels comparison diagram after control group in the embodiment of the present invention 1 and strengthening；
Fig. 2 is the artemia micronutrient levels comparison diagram after control group in the embodiment of the present invention 2 and strengthening；
Fig. 3 be the embodiment of the present invention 3 in for strengthening Macrobrachium nipponensis young control group with strengthening after young survival rate comparison diagram.
Specific embodiment：
Embodiment 1
A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis, concrete grammar comprises the following steps：
Step one, is Rich in Trace Element carrier using high enrichment chlorella, and wherein the weight in wet base of chlorella is 7 × 1010
cells g−1；
Step 2, compound concentration is 1000 mg L−1Copper-bath and concentration be 500 mg L-1Solution of zinc sulfate；
Step 3, takes high enrichment chlorella 10 g of step one to add the 3 ml copper-baths and 3 that step 2 prepares
Carry out Rich in Trace Element copper and zinc in the mixed solution of ml solution of zinc sulfate, be enriched with 1-3h, the seawater bead of survival or death
Algae all can be enriched with appropriate concentration trace copper and zinc；
Step 4, the chlorella after step 2 Rich in Trace Element copper and zinc is taken out；Put in the tank that cultivation has artemia, with
When add emulsifying fish oil and yeast selenium that artemia is strengthened, strengthening process temperature is 28 ± 0.5 DEG C, pH 7.8-8.2, molten
Solution oxygen is more than 6 mg/L, salinity 20 ‰, and the amount of addition emulsifying fish oil and yeast selenium is equal with the mass volume ratio of tank culturing liquid
For 2mg/L, enhanced time 8-12h.
Nutrient mensure is carried out to the artemia after above strengthening, is contrasted with the control group strengthened simultaneously,
Result sees Fig. 1 it can be seen that the artemia copper coin cellulose content after strengthening has reached 35（μ g/g dry）Left and right, does not strengthen
More than 3 times of control group content, the content nearly 60 of Zn-ef ficiency（μ g/g dry）, it is 2 times of the control group content do not strengthened
More than, and in the control group do not strengthened, do not detect the content of selenium element, can substantially detect in the artemia after strengthening
To selenium element content.
Embodiment 2
A kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis, concrete grammar comprises the following steps：
Step one, is Rich in Trace Element carrier using high enrichment chlorella, and wherein the weight in wet base of chlorella is 7 × 1010
cells g−1；
Step 2, compound concentration is 500 mg L−1Copper-bath and concentration be 200 mg L-1Solution of zinc sulfate；
Step 3, takes high enrichment chlorella 10 g of step one to add the 5 ml copper-baths and 5 that step 2 prepares
Carry out Rich in Trace Element copper and zinc in the mixed solution of ml solution of zinc sulfate, be enriched with 1-3h, the seawater bead of survival or death
Algae all can be enriched with appropriate concentration trace copper and zinc；
Step 4, the chlorella after step 2 Rich in Trace Element copper and zinc is taken out；Put in the tank that cultivation has artemia, with
When add emulsifying fish oil and yeast selenium that artemia is strengthened, the process of strengthening is carried out in two stages, the first stage be by
It is passed through oxygen in tank and system temperature is warming up to 30-32 DEG C, keep 1-2h, be subsequently adding emulsifying fish oil, add emulsification
Type fish oil is 1mg/L with the mass volume ratio of tank culturing liquid, keeps 1-2h, then adds yeast selenium, keep 1-2h, adds
The amount of yeast selenium is identical with the amount of emulsifying fish oil；Then system temperature is reduced to 15-18 DEG C, keeps 8-10h, enter second-order
Section, adds emulsifying fish oil and yeast selenium, the emulsifying fish oil of addition and ferment under conditions of keeping system temperature to be 15-18 DEG C
The mass volume ratio that the amount of female selenium cultivates liquid system with tank is 1mg/L, and after addition, maintenance system dissolved oxygen amount is more than 6mg/L,
PH7.8-8.2, salinity 20 ‰, in 3-4h, tank culturing liquid system temperature is risen to 30 DEG C, keep 1-2h, strengthening completes.
Nutrient detection is carried out for the artemia after above strengthening, and is contrasted with not doing the control group strengthened, knot
Fruit sees Fig. 2 it can be seen that the artemia copper coin cellulose content after strengthening has exceeded 40（μ g/g dry）, be 4 times of control group with
On, after strengthening, artemia Zn-ef ficiency content has reached 70（μ g/g dry）More than, it is more than 3 times of the control group do not strengthened, and
The content of selenium also becomes apparent from.
The present embodiment compared with Example 1, is changed, wherein this reality on the intensifying method to artemia for the trace element
Apply example and employ two stages and artemia is strengthened, by final result as can be seen that in artemia body after the present embodiment strengthening
Micronutrient levels strengthen apparently higher than the mode according to embodiment 1 after micronutrient levels.Pass through two in the present embodiment
Stage strengthens to artemia, improves the absorption to nutrient for the artemia, increased artemia endotrophic element after strengthening
Content, improves quality, can preferably as nutrient enrichment fodder, the Macrobrachium nipponensis young be strengthened.
Embodiment 3
In industrial seedling rearing, after method strengthening as described in above example for the artemia, the Macrobrachium nipponensis young is fed daily
Artemia 20-30/L, feeds 22 days altogether.The development condition of the young shows, with control group be feed strengthening artemia compared with, nutrition is strong
Artemia after change has significant facilitation to the survival rate of the Macrobrachium nipponensis young, can promote the molt metamorphosis of the young, and then
Reduce the generation of freshwater shrimp young autotomy phenomenon.As shown in figure 3, the young survival rate after strengthening is with respect to the comparison composition for strengthening
Motility rate improves more than 15%.
Claims (9)
1. a kind of micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis is it is characterised in that concrete grammar includes
Following steps：
Step one, is Rich in Trace Element carrier using high enrichment chlorella；
Step 2, compound concentration is 500-1000 mg L−1Copper-bath and concentration be 200-500 mg L-1Zinc sulfate
Solution；
Step 3, takes the high enrichment chlorella of step one to add the copper-bath for preparing of step 2 and solution of zinc sulfate
Carry out Rich in Trace Element copper and zinc in mixed solution；
Step 4, the chlorella after step 2 Rich in Trace Element copper and zinc is taken out；Put in the tank that cultivation has artemia, with
When add emulsifying fish oil and yeast selenium that artemia is strengthened；
Step 5, carries out feeding using the artemia after strengthening to the Macrobrachium nipponensis young.
2. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In the weight in wet base configuring chlorella in step one is 7 × 1010cells g−1.
3. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In 10 g chlorellas being added in 3-5ml copper-bath and the mixed liquor of 3-5ml solution of zinc sulfate in step 3.
4. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In in step 3, the enrichment time of Rich in Trace Element copper and zinc is 1-3h.
5. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In, in step 4 strengthening temperature be 28 ± 0.5 DEG C, pH 7.8-8.2, dissolved oxygen be more than 6 mg/L, salinity 20 ‰.
6. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In in step 4, the addition of emulsifying fish oil is 2mg/L.
7. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In in step 4, the addition of yeast selenium is 2mg/L.
8. the micro-element nutrition intensifying method of the promotion Macrobrachium nipponensis paedomorphosis according to claim 6 or 7, its feature
It is, in step 4, enhanced time is 8-12h.
9. the micro-element nutrition intensifying method promoting Macrobrachium nipponensis paedomorphosis according to claim 1, its feature exists
In adding the process that emulsifying fish oil and yeast selenium are strengthened to artemia to carry out in two stages in step 4, the first stage
It is will to be passed through oxygen in tank and system temperature is warming up to 30-32 DEG C, keep 1-2h, be subsequently adding emulsifying fish oil, add
Emulsifying fish oil is 1mg/L with the mass volume ratio of tank culturing liquid, keeps 1-2h, then adds yeast selenium, keeps 1-2h,
Add the amount of yeast selenium identical with the amount of emulsifying fish oil；Then system temperature is reduced to 15-18 DEG C, keeps 8-10h, enter the
Two-stage, under conditions of keeping system temperature to be 15-18 DEG C, add emulsifying fish oil and yeast selenium, the emulsifying fish oil of addition
The mass volume ratio cultivating liquid system with tank with the amount of yeast selenium is 1mg/L, and after addition, maintenance system dissolved oxygen amount is more than
6mg/L, pH7.8-8.2, salinity 20 ‰, in 3-4h, tank culturing liquid system temperature is risen to 30 DEG C, keep 1-2h, strengthened
Become.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110999836A (en) * | 2019-12-31 | 2020-04-14 | 海南晨海水产有限公司 | Nutrition strengthening method for promoting growth of penaeus orientalis larvae |
| CN112913727A (en) * | 2021-01-28 | 2021-06-08 | 海南晨海水产有限公司 | Nutrition strengthening method for promoting development of erythema erythropolis |
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| EP1049385B1 (en) * | 1998-01-21 | 2003-06-18 | University of Maryland Biotechnology Institute | Methods for the enrichment of live feed for fish larvae with docosahexaenoic acid |
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| CN112913727A (en) * | 2021-01-28 | 2021-06-08 | 海南晨海水产有限公司 | Nutrition strengthening method for promoting development of erythema erythropolis |
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