CN101260368A - Chinese caterpillar fungus liquid bacterial cultivating matrix - Google Patents
Chinese caterpillar fungus liquid bacterial cultivating matrix Download PDFInfo
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- CN101260368A CN101260368A CNA200810011010XA CN200810011010A CN101260368A CN 101260368 A CN101260368 A CN 101260368A CN A200810011010X A CNA200810011010X A CN A200810011010XA CN 200810011010 A CN200810011010 A CN 200810011010A CN 101260368 A CN101260368 A CN 101260368A
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Abstract
The invention relates to a cordyceps liquid spawn culture medium, which is mixed and prepared by 30 to 100 gram glucose, 3 to 10 gram peptone, 0.5 to 1.1 gram multiplex vitamin, 5 to 12 gram multiplex mineral matters and 961.5 to 876.9 gram water, wherein, the vitamin mainly comprises VA, VB1, VB2, VB5, VB12, VC and VE which are prepared according to the ratio of 1 to 1; the mineral matters mainly comprise calcium hypochlorite, green vitriol, zinc selenides, sodium chlorides, potassium nitrates, magnesium sulphates, manganese sulphates, ammonia sulphates and potassium iodides which are prepared according to the ratio of 1 to 1 or are mixed and prepared by 2 gram calcium hypochlorite, 0.2 gram green vitriol, 0.2 gram zinc selenides, 0.5 to 1 gram sodium chlorides, 3 gram potassium nitrates, 3 gram magnesium sulphates, 0.2 gram manganese sulphates, 0.3 gram potassium iodides and 2 gram ammonia sulphates. The invention concentrates effective nutrition and medicinal compositions in cordyceps, and reduces the dosage but does not reduce the effect. The cordyceps liquid spawn culture medium can save 90 percent labor and save 90 percent energy, is environment-friendly without refuses; moreover, the seed production technology is simplified, which contributes to the popularity and promotion of cordyceps seed production and culture technologies and is favorable for the development of cordyceps project and simultaneously avoids large-area pollution loss due to various reasons of liquid spawn.
Description
Technical field
The present invention relates to a kind of mushroom production matrix, specifically relate to a kind of culture medium that is used for the Chinese caterpillar fungus liquid spawn.
Background technology
Cordyceps sinensis is used as the treasured in China's Chinese medicine treasure-house always.Scientific research in recent years finds that Chinese caterpillar fungus can be improved body's immunological function, be had and strengthen the body resistance to consolidate the constitution, protection heart, liver, attenuation, enhancing body self anti-cancer ability and effect anti-ageing, the skin moisten beauty treatment.
Along with continuous expansion to Cordyceps sinensis understanding field; demand to Chinese caterpillar fungus; not only the domestic market enlarges day by day; and the demand of world markets such as South East Asia, Japan, the U.S. is also increasing; natural cordyceps has belonged to country and has forbidden that a class of plucking protects medicinal species, and raw material sources have become the key that can this kind further enlarge market.Therefore replacing wild Chinese caterpillar fungus with the artificial aweto cultivation, is the protection natural resources of Chinese medicinal materials, promotes sustainable development the needs of preserving the ecological environment.
Along with the continuous development and the popularization of science and technology, the artificial culture Chinese caterpillar fungus has become an industry, and is just in the ascendant.Increasing people goes on the road of building up the family fortunes by the cultivation Chinese caterpillar fungus.Because liquid spawn is adopted in the artificial aweto cultivation, the culture medium that bacterial classification is used is the key of main pharmacodynamics composition cordycepin in the decision artificial aweto, cordycepic acid, Cordyceps polysaccharide, SOD content height.Adopt Cordyceps sinensis (be called for short Chinese caterpillar fungus) efficacy component cordycepin, cordycepic acid, Cordyceps polysaccharide, the SOD content of known PDA cordyceps species substratum low, the micro-tungsten of needed by human body, iron, zinc, magnesium, manganese, selenium, potassiumiodide equal size are also low.Therefore, drug effect is relatively poor, and nutritive value is lower.
Summary of the invention
The purpose of this invention is to provide a kind of efficacy component cordycepin, cordycepic acid, SOD, Cordyceps polysaccharide content height, and be rich in the Chinese caterpillar fungus liquid bacterial cultivating matrix of the trace element of multiple human body needs.
The technical scheme that adopts is:
Chinese caterpillar fungus liquid bacterial cultivating matrix is by glucose, peptone, V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EBe hybridly prepared into Deng several mineral materials such as multivitamin and Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide and water.
With preparation 1000g Chinese caterpillar fungus liquid bacterial cultivating matrix is example, and its ratio of components is:
Glucose 30-100g, peptone 3-10g, multivitamin 0.5-1.1g, several mineral materials 5-12g and water 961.5-876.9g.
The compound method of Chinese caterpillar fungus liquid bacterial cultivating matrix is:
Get by weight percentage glucose 30-100g, peptone 3-10g, VITAMIN 0.5-1.1g, mineral substance 5-12g be placed on be mixed in the container even, in container, add an amount of 80-100 ℃ hot water again, material is dissolved, add water to 1000g again, again thorough mixing evenly after, be divided in the vial by setting metering, seal, be finished product with the poly-third OPP plastic film.
Described VITAMIN mainly contains V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
E, each VITAMIN was by preparation in 1: 1.
Described mineral substance mainly contains Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each mineral element is by preparation in 1: 1 or Losantin 2g, ferrous sulfate 0.2g, zinc selenide 0.2g, sodium-chlor 0.5-1g, saltpetre 3g, sal epsom 3g, manganous sulfate 0.2g, potassiumiodide 0.3g, sulfate of ammoniac 2g.
Use Chinese caterpillar fungus liquid bacterial cultivating matrix of the present invention, through sterilization, cooling (writing out cooling temperature), insert cordyceps species, cultivate (leave standstill, all can vibrate), resulting liquid cordyceps species is transferred and is expanded numerously to rice medium, and the artificial aweto that grows out is exactly the high Chinese caterpillar fungus of cordycepin equal size.
This patent advantage is:
1, concentrates effective nutrition of Chinese caterpillar fungus and medicinal component, reduce consumption and do not reduce effect.
2, in bacterial classification production, save in the medium preparation process artificial 90%, save the energy 90%, environmental protection does not have rubbish.
3, the Chinese caterpillar fungus seeding technique is oversimplified, be convenient to popularizing and promoting of the Chinese caterpillar fungus production of hybrid seeds and cultivation technique, help the development of Chinese caterpillar fungus cause.
4, bacterial classification expense and freight charges are bought in income, the minimizing that increases the Chinese caterpillar fungus producer, also avoid causing because of a variety of causes of liquid spawn simultaneously the loss of pollution in wide area.
This patent is applicable to that the liquid spawn of Cordyceps sporophore produces and the fermentation culture production of Cordyceps mycelium.
Table one compares with Cordyceps sinensis nutrition to the Chinese caterpillar fungus (to call the matrix Chinese caterpillar fungus in the following text) of table four for producing with Chinese caterpillar fungus liquid bacterial cultivating matrix of the present invention.
Entrust Shenyang Branch of Chinese Academy of Sciences physical and chemical testing center to measure.The interpretation of result of measuring is compared as follows:
One, special component content relatively
Measurement result shows, cordycepin is that 8.5 times of Cordyceps sinensis content, cordycepic acid are 2.14 times of Cordyceps sinensis in the matrix Chinese caterpillar fungus.SOD content height is 3 times of Cordyceps sinensis, and the content of Cordyceps polysaccharide is less than Cordyceps sinensis only.
Two, aminoacids content relatively
Measurement result shows that all than high several times to tens times of Cordyceps sinensis, Guang amino acid is a little less than Cordyceps sinensis only for 17 seed amino acids of matrix Chinese caterpillar fungus, and the 18 seed amino acid total contents of matrix Chinese caterpillar fungus are 7.76 times than Cordyceps sinensis.
Three, vitamin contents relatively
Measurement result shows, the vitamin contents of matrix Chinese caterpillar fungus is all than Cordyceps sinensis height, V
AHigh several times, V
B2, V
B12, V
CHigh tens times, V
B1, V
EHigh hundred times, V
B6Exceed thousand times.
Four, content of mineral substances relatively
Measurement result shows, the Na of matrix Chinese caterpillar fungus, Mg, Co, Cr content are a little less than Cordyceps sinensis, the K of matrix Chinese caterpillar fungus, P, Fe, Mo content are a little more than Cordyceps sinensis, and the Se content of matrix Chinese caterpillar fungus is higher than nearly 3 times of Cordyceps sinensis, and the Zn of matrix Chinese caterpillar fungus, Cu content are higher than Cordyceps sinensis more than 5 times.
Embodiment
Embodiment one
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be mixed with by glucose 30g, peptone 3g, VITAMIN 0.5g, mineral substance 5g and water 961.5g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
Embodiment two
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be mixed with by glucose 40g, peptone 4g, VITAMIN 0.54g, mineral substance 5.1g and water 950.36g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
Embodiment three
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be mixed with by glucose 50g, peptone 5.1g, VITAMIN 0.6g, mineral substance 5.6g and water 938.7g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EBe hybridly prepared into, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
Embodiment four
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be mixed with by glucose 90g, peptone 8g, VITAMIN 0.9g, mineral substance 5.9g and water 895.2g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
Embodiment five
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be hybridly prepared into by glucose 100g, peptone 10g, VITAMIN 1g, mineral substance 10g and water 879g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
Embodiment six
Chinese caterpillar fungus liquid bacterial cultivating matrix is to be hybridly prepared into by glucose 100g, peptone 10g, VITAMIN 1.05g, Losantin 2g, ferrous sulfate 0.2g, zinc selenide 0.2g, sodium-chlor 1g, saltpetre 3g, sal epsom 3g, manganous sulfate 0.2g, sulfate of ammoniac 2g and water 877.35g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1.
Claims (7)
1, Chinese caterpillar fungus liquid bacterial cultivating matrix is characterized in that being hybridly prepared into by glucose 30-100g, peptone 3-10g, multivitamin 0.5-1.1g, several mineral materials 5-12g and water 961.5-876.9g; Described VITAMIN mainly contains V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EBy preparation in 1: 1; Mineral substance mainly contains Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide and is hybridly prepared into by 1: 1 or Losantin 2g, ferrous sulfate 0.2g, zinc selenide 0.2g, sodium-chlor 0.5-1g, saltpetre 3g, sal epsom 3g, manganous sulfate 0.2g, potassiumiodide 0.3g, sulfate of ammoniac 2g.
2, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being mixed with by glucose 30g, peptone 3g, VITAMIN 0.5g, mineral substance 5g and water 961.5g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
3, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being mixed with by glucose 40g, peptone 4g, VITAMIN 0.54g, mineral substance 5.1g and water 950.36g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
4, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being mixed with by glucose 50g, peptone 5.1g, VITAMIN 0.6g, mineral substance 5.6g and water 938.7g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EBe hybridly prepared into, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
5, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being mixed with by glucose 90g, peptone 8g, VITAMIN 0.9g, mineral substance 5.9g and water 895.2g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
6, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being hybridly prepared into by glucose 100g, peptone 10g, VITAMIN 1g, mineral substance 10g and water 879g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1; Mineral substance is made up of Losantin, ferrous sulfate, zinc selenide, sodium-chlor, saltpetre, sal epsom, manganous sulfate, sulfate of ammoniac, potassiumiodide, and each component was by preparation in 1: 1.
7, Chinese caterpillar fungus liquid bacterial cultivating matrix according to claim 1 is characterized in that being hybridly prepared into by glucose 100g, peptone 10g, VITAMIN 1.05g, Losantin 2g, ferrous sulfate 0.2g, zinc selenide 0.2g, sodium-chlor 1g, saltpetre 3g, sal epsom 3g, manganous sulfate 0.2g, sulfate of ammoniac 2g and water 877.35g; VITAMIN is by V
A, V
B1, V
B2, V
B5, V
B12, V
C, V
EForm, each component was by preparation in 1: 1.
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|---|---|---|---|
| CNA200810011010XA CN101260368A (en) | 2008-04-14 | 2008-04-14 | Chinese caterpillar fungus liquid bacterial cultivating matrix |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200810011010XA CN101260368A (en) | 2008-04-14 | 2008-04-14 | Chinese caterpillar fungus liquid bacterial cultivating matrix |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630490A (en) * | 2012-04-19 | 2012-08-15 | 鲁东大学 | Artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
| CN103483037A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
| CN105154491A (en) * | 2015-09-02 | 2015-12-16 | 天津现代职业技术学院 | Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides |
| CN105746172A (en) * | 2016-02-23 | 2016-07-13 | 张家港顺泰元生物科技有限公司 | Selenium-enriched cordyceps culture method |
-
2008
- 2008-04-14 CN CNA200810011010XA patent/CN101260368A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630490A (en) * | 2012-04-19 | 2012-08-15 | 鲁东大学 | Artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content |
| CN103396193A (en) * | 2013-08-08 | 2013-11-20 | 董亚贤 | Cordycepsmilitaris strain liquid culture medium and preparation method therefor |
| CN103483037A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
| CN103483037B (en) * | 2013-09-05 | 2015-06-24 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
| CN105154491A (en) * | 2015-09-02 | 2015-12-16 | 天津现代职业技术学院 | Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides |
| CN105154491B (en) * | 2015-09-02 | 2018-08-24 | 天津现代职业技术学院 | A kind of culture medium for cordyceps sinensis liquid state fermentation production exocellular polysaccharide |
| CN105746172A (en) * | 2016-02-23 | 2016-07-13 | 张家港顺泰元生物科技有限公司 | Selenium-enriched cordyceps culture method |
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Application publication date: 20080910 |