CN106386510A - Manufacturing method of Anoectochilus roxburghii test tube flowers - Google Patents
Manufacturing method of Anoectochilus roxburghii test tube flowers Download PDFInfo
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- CN106386510A CN106386510A CN201611108158.6A CN201611108158A CN106386510A CN 106386510 A CN106386510 A CN 106386510A CN 201611108158 A CN201611108158 A CN 201611108158A CN 106386510 A CN106386510 A CN 106386510A
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- test tube
- herba anoectochili
- anoectochili roxburghii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention relates to a manufacturing method of Anoectochilus roxburghii test tube flowers. The manufacturing method uses Anoectochilus roxburghii sterile plants as materials and comprises the following steps: preparation of colored culture medium; implantation of Anoectochilus roxburghii sterile plants; and rooting culture and plant observation. The method is simple to operate and low in production cost. The produced product is delicate, is free from the influence of the seasons and regional environment, only needs lighting, does not need watering, and has long ornamental period (up to 10-18 months). The Anoectochilus roxburghii plants have the advantages of graceful shape, special leaf color and veins, high ornamental value and longer ornamental period, can beautify the living environment of people, and can save the time of people for taking care of the plants.
Description
Technical field
The invention belongs to plant tissue culture and test tube field of flower technology, it is related to a kind of container culture side of Herba Anoectochili roxburghii
Method, particularly to a kind of manufacture method of Herba Anoectochili roxburghii test tube flowers.
Background technology
Herba Anoectochili roxburghii, also known as Anoectochilus nefiliforme (Nakai) hara（Scientific name：Anoectochilus roxburghii(Wall.) Lindl.）.Property happiness
The moon, avoids direct sunlight, and happiness is moistening, avoids drying, 15 DEG C the most preferably grow to 30 DEG C.More than 35 DEG C undergrowths.Plant is high 8-18 li
Rice.Root stock is crawled, elongation, meat, tool section, and section is taken root.Stem is upright, meat, cylindrical, has 3-4 piece of leaf.Blade oval
Or avette, long 1.3-3.5 centimetre, wide 0.8-3 centimetre, above mulberry or black purple, tool golden red carry silky lustre U.S.
Beautiful net vein, back side lilac red, tip is closely anxious sharp or slightly blunt, the nearly section shape of base portion or circle, narrow one-tenth handle suddenly；Petiole long 4-10 milli
Rice, base portion is expanded into the sheath embracing stem.Herba Anoectochili roxburghii plant is stood upright delicate, and the vein exquisiteness of blade is good-looking, and ornamental value is high, except suitable
With with potted plant plantation, be also suitable for being fabricated to test tube flowers, not only save placing space, be houseplant ornaments only choosing
Select, increase indoor beautification, simultaneously new and original, arrange conveniently, do not affected by seasonal climate.Additionally, Herba Anoectochili roxburghii also has
Very high medical value：Clearing away heat and cooling blood, removing toxic substances and promoting subsidence of swelling, nourishing the lung to arrest cough.For spitting blood, phlegm dyspnea of coughing, difficulty and pain in micturition, quench one's thirst, breast
Rotten urine, pediatric acute convulsion, boil on the nape opposite the mouth, heart disease, venom.
Test tube flowers are using modern plants tissue culture technique, by the tune to totally-enclosed Tube propagation and condition of culture
Control, manufactured mini test tube flowers.Test tube flowers belong to plant tissue culture technique category, and this technology is thin according to plant
This theory of the Almightiness type of born of the same parents, isolates the tissue suiting the requirements, organ or cell from plant, and protoplast etc., by no
Bacterium operates, and is seeded in the complete plant being cultivated and being obtained regeneration in the culture medium containing various nutrient substance and phytohormone
Strain or the technology producing other products with economic worth.
Test tube flowers are in the starting stage in the research of China, and the test tube flower of multiple flowers is in experimental study, but
As the research of test tube flowers, report is had no for Herba Anoectochili roxburghii.
Content of the invention
It is an object of the invention to provide a kind of manufacture method of Herba Anoectochili roxburghii test tube flowers, the method operates simply, culture
Cycle is short, the Herba Anoectochili roxburghii test tube flower ornamental phase obtaining is long, has higher economy and ornamental value.
The manufacture method of Herba Anoectochili roxburghii test tube flowers of the present invention comprises the following steps：
A. colored culture medium is prepared：Prepare the culture medium of clear, colorless, heating based on MS medium component table, be subsequently adding
The pigment of the different colours of variable concentrations, carries out containers, sterilizing, and natural cooling obtains colored culture medium；
B. the implantation of the aseptic plant of Herba Anoectochili roxburghii：Herba Anoectochili roxburghii that choose health, that growing way identical has been obtained by Fast-propagation
Aseptic plant, as inoculation material, covers bottle cap or the bottle stopper of sterilizing at once after inoculation, with least 2 layers sealed membrane sealing；
C. root culture and plant are observed：Postvaccinal plant, through 1-2 week culture, free of contamination test tube flowers are into
Product, this Herba Anoectochili roxburghii test tube flower ornamental is high, viewing period 10-18 month.
Preferably, in described step A, described colour culture medium is：1/2MS+3% sucrose+0.4% carrageenan, pH value is
5.8-6.2, food coloring 0g/L-30g/L.
Preferably, described food coloring is to be selected from：Lemon yellow, sunset yellow, amaranth, erythrosine, indigo, temptation red
Kind or the combination of more than one pigments.
Preferably, in described step B, the Herba Anoectochili roxburghii upgrowth situation of selected inoculation：The long 2.0-5.0cm of strain, the number of blade
2-6 piece.
Preferably, in described step B, after covering bottle cap or bottle stopper, with least 2 layers sealed membrane sealing.
The manufacture method of Herba Anoectochili roxburghii test tube flowers of the present invention is simple to operate, low production cost, the product being produced
Exquisiteness, not season and territorial environment affected it is only necessary to illumination is it is not necessary to water, viewing period was long, up to 10-18 month.This
The form of Herba Anoectochili roxburghii plant out made by invention is graceful, and leaf color vein especially, has the blade of exquisiteness and graceful plant type,
There is high ornamental value, definitely no less than the plant that blooms.Therefore, the Herba Anoectochili roxburghii that manufacture method of the present invention obtains
Test tube flowers have high ornamental and longer viewing period, can not only beautify the living environment of people moreover it is possible to save people
Time is arranged to plant.
Specific embodiment
The following is the specific embodiment of production technology of the present invention, technical scheme is done and is described further, but
It is that protection scope of the present invention is not limited to these embodiments.Every change without departing substantially from present inventive concept or equivalent substitute are all wrapped
Include within protection scope of the present invention.
Embodiment one：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.300ml distilled water, adds 30g sucrose, 2.37g MS dry powder, plus distilled water is extremely
1000ml, adjusts pH value 5.8, adds 4g carrageenan, heated and boiled, adds pigment temptation red.The culture medium preparing is carried out point
Dress, is sub-packed in the ornamental type container such as mini test tube, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.Sterilizing
After end, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 14 days, plant is not contaminated Herba Anoectochili roxburghii, this test tube flower is cultivated successfully, can be packed or directly go out
Sell.Wherein, the time average that plant starts to grow new root on luring red culture medium is 7.5 days, plant average height when 8 weeks
4.3cm, plant height average height 6.6cm when 28 weeks.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.Most preferably view and admire
After phase was for inoculation the 4th week, now Herba Anoectochili roxburghii plant appearance is exquisite, has higher sight.
Embodiment two：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Take 200ml distilled water, add 15g sucrose, 1.19gMS dry powder, plus distilled water is settled to
Culture medium cumulative volume 500ml, adjusts pH value 6.2, adds 2g carrageenan, heated and boiled, adds pigment indigo.By the colour preparing
Culture medium carries out subpackage, is sub-packed in the ornamental type container such as mini test tube, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes
Autoclaving.After sterilizing terminates, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation at once, sealed with 3 layers of sealing compound
Mouthful, carry out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 10 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 4th day, Herba Anoectochili roxburghii plant mean height 5.2cm when 12 weeks.In test tube, Herba Anoectochili roxburghii
Trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii plant appearance is exquisite, has higher
Sight.
Embodiment three：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
6.0, add the carrageenan of respective amount, heated and boiled, add appropriate pigment erythrosine.The colored culture medium preparing is carried out point
Dress, is sub-packed in the ornamental type container such as mini test tube, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.Sterilizing
After end, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 12 days, if during this period, be not contaminated Herba Anoectochili roxburghii plant, as qualified test tube flowers, can carry out
Pack or sell.When cultivating 6 weeks, rooting rate is 100%, average plant height 4.5cm when cultivating 12 weeks.In glass container, gold
Anoectochilus roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii plant appearance is exquisite, has relatively
High sight.
Example IV：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
6.0, add the carrageenan of respective amount, heated and boiled, add appropriate pigment sunset yellow, lure red, indigo, amaranth, lemon yellow
The complex being formed, final color is brown.The colored culture medium preparing is carried out subpackage, is sub-packed in mini test tube etc. and views and admires
In type container, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.After sterilizing terminates, culture medium natural cooling,
Use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation at once, sealed with 4 layers of sealing compound
Mouthful, carry out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 14 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 4th day, Herba Anoectochili roxburghii plant mean height 5cm when 12 weeks.In test tube, Herba Anoectochili roxburghii is given birth to
For a long time up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii plant appearance is exquisite, has higher sight
Reward property.
Embodiment five：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
6.0, add the carrageenan of respective amount, heated and boiled, add appropriate pigment sunset yellow.The colored culture medium preparing is carried out point
Dress, is sub-packed in the ornamental type container such as mini test tube, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.Sterilizing
After end, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 14 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 4th day, and rooting rate reaches 100% within the 5th week, Herba Anoectochili roxburghii plant mean height when 12 weeks
4.3cm.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii is planted
Strain appearance is exquisite, has higher sight.
Embodiment six：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
6.0, add the carrageenan of respective amount, heated and boiled, add appropriate pigment amaranth, lemon yellow and indigo formation pigment to be combined
Thing, final color is greyish purple.The colored culture medium preparing is carried out subpackage, is sub-packed in the ornamental type container such as mini test tube,
The good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.After sterilizing terminates, culture medium natural cooling, make when to be seeded
With.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation at once, sealed with 3 layers of sealing compound
Mouthful, carry out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 12 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 5th day, and rooting rate reaches 100% within the 9th week, Herba Anoectochili roxburghii plant mean height when 12 weeks
4.6cm.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii is planted
Strain appearance is exquisite, has higher sight.
Embodiment seven：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
6.0, add the carrageenan of respective amount, heated and boiled, add appropriate pigment lemon yellow and indigo formation pigment complex, final face
Color is apple green.The colored culture medium preparing is carried out subpackage, is sub-packed in the ornamental type container such as mini test tube, subpackage is good
Culture medium carries out 121 DEG C, 20 minutes autoclavings.After sterilizing terminates, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 14 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 5th day, and rooting rate reaches 100% within the 6th week, Herba Anoectochili roxburghii plant mean height when 12 weeks
4.3cm.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii is planted
Strain appearance is exquisite, has higher sight.
Embodiment eight：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
5.8, add the carrageenan of respective amount, heated and boiled, add appropriate pigment lemon yellow and indigo formation pigment complex, final face
Color is apple green.The colored culture medium preparing is carried out subpackage, is sub-packed in the ornamental type container such as mini test tube, subpackage is good
Culture medium carries out 121 DEG C, 20 minutes autoclavings.After sterilizing terminates, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 10 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 4th day, and rooting rate reaches 100% within the 9th week, Herba Anoectochili roxburghii plant mean height when 12 weeks
5.2cm.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii is planted
Strain appearance is exquisite, has higher sight.
Embodiment nine：The making of Herba Anoectochili roxburghii test tube flowers
1. material：The healthy and strong Herba Anoectochili roxburghii plant of asepsis growth.
2. test tube flowers Making programme：
(1) material prepares.The Herba Anoectochili roxburghii plant taking health carries out aseptic culture, Fast-propagation, treats that Herba Anoectochili roxburghii plant grows new root,
Obtain batch Herba Anoectochili roxburghii aseptic plant, the production material of the test tube flowers of the present invention is provided.
(2) inoculation proceeds to the ornamental type container such as mini test tube.
1. colored culture medium is prepared.Formula according to 1/2MS+3% sucrose+0.4% carrageenan prepares culture medium, adjusts pH value
5.8, the carrageenan of addition respective amount, heated and boiled, without pigment, final clear, colorless.The culture medium preparing is carried out
Subpackage, is sub-packed in the ornamental type container such as mini test tube, the good culture medium of subpackage is carried out 121 DEG C, 20 minutes autoclavings.Go out
After bacterium is terminated, culture medium natural cooling, use when to be seeded.
2. inoculate.Aseptic operating platform is carried out, by flow process（1）The Herba Anoectochili roxburghii aseptic plant sections that Fast-propagation obtains
Access process（2）In in the colored culture medium that obtains, cover bottle cap or the bottle stopper of sterilizing after inoculation, with least 2 layers sealing at once
Glue seals, and carries out seal operation.
（3）Cultivate.The Herba Anoectochili roxburghii test tube flowers that inoculation is finished are transferred to culturing room, 25 ± 2 DEG C of cultures of temperature, every daylight
According to 16h, observe 14 days, if Herba Anoectochili roxburghii plant is not contaminated, this test tube flower is cultivated successfully, can be packed or directly
Sell.Wherein, Herba Anoectochili roxburghii plant starts long root on the 4th day, and rooting rate reaches 100% within the 6th week, Herba Anoectochili roxburghii plant mean height when 12 weeks
4.9cm.In test tube, Herba Anoectochili roxburghii trophophase was up to more than 18 months.After best-looking states were for inoculation the 4th week, now Herba Anoectochili roxburghii is planted
Strain appearance is exquisite, has higher sight.
Claims (5)
1. a kind of manufacture method of Herba Anoectochili roxburghii test tube flowers is it is characterised in that comprise the following steps：
A. colored culture medium is prepared：Prepare the culture medium of clear, colorless, heating based on MS medium component table, be subsequently adding
The pigment of the different colours of variable concentrations, carries out containers, sterilizing, and natural cooling obtains colored culture medium；
B. the implantation of the aseptic plant of Herba Anoectochili roxburghii：Herba Anoectochili roxburghii that choose health, that growing way identical has been obtained by Fast-propagation
Aseptic plant, as inoculation material, covers bottle cap or the bottle stopper of sterilizing at once after inoculation, with least 2 layers sealed membrane sealing；
C. root culture and plant are observed：Postvaccinal plant, through 1-2 week culture, free of contamination test tube flowers are into
Product, this Herba Anoectochili roxburghii test tube flower ornamental is high, viewing period 10-18 month.
2. the manufacture method of Herba Anoectochili roxburghii test tube flowers according to claim 1 is it is characterised in that in described step A, described
Colored culture medium is：1/2MS+3% sucrose+0.4% carrageenan, pH value is 5.8-6.2, food coloring 0g/L-30g/L.
3. the manufacture method of Herba Anoectochili roxburghii test tube flowers according to claim 2 is it is characterised in that described food coloring is choosing
From：Lemon yellow, sunset yellow, amaranth, erythrosine, combination that is indigo, luring one or more red pigments.
4. the manufacture method of Herba Anoectochili roxburghii test tube flowers according to claim 1 is it is characterised in that in described step B, selected
The Herba Anoectochili roxburghii upgrowth situation of the inoculation taking：The long 2.0-5.0cm of strain, number of blade 2-6 piece.
5. the manufacture method of Herba Anoectochili roxburghii test tube flowers according to claim 1 is it is characterised in that in described step B, cover
After bottle cap or bottle stopper, with least 2 layers sealed membrane sealing.
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