CN106383087A - Polarization optical coherence tomography/polarization multiphoton microscopy bimodal imaging system - Google Patents
Polarization optical coherence tomography/polarization multiphoton microscopy bimodal imaging system Download PDFInfo
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Abstract
The invention relates to the fields of biomedicine and optics, and aims to provide a polarization optical coherence tomography/polarization multiphoton microscopy bimodal imaging system which comprises a control and processing module, a polarization optical coherence tomography imaging module, a polarization multiphoton microscopy imaging module and a scanning module. The parameters of structural information, spectral information and optical characteristics of the sample identical information range bimodal image are simultaneously acquired. The polarization optical coherence tomography and polarization multiphoton microscopy image techniques with different functions are integrated to implement parallel application and fusion of two image modes; the polarization characteristics can be utilized to effectively obtain the properties of biological tissues with birefringent characteristics, thereby implementing the three-dimensional superposed images of the biological tissues and the pathological, spectral and birefringent properties; and the system evaluates the pathological characteristics of the tissues according to the longitudinal/transverse bimodal characteristics, and thus, is a real-time nondestructive dynamic bimodal imaging system.
Description
Technical field
The present invention relates to biomedical and optical field, particularly to a kind of polarization optics coherent tomographic and polarization multi-photon
Micro- double-mode imaging system.
Background technology
For the detection method of biological tissue's hyperfine structure, mainly there are ultramicroscope, X-ray, biochemistry at present
Analysis, histopathological analysis, multiphoton fluorescence technology etc..Wherein ultramicroscope, X-ray, biochemical analysiss, tissue
The maximum shortcoming of the methods such as pathological analysis be have serious damaging, and cannot in real time, the detection of live body.And have can be fast
Speed realizes the Optical Coherence Tomography Imaging Technology of structure imaging and functional imaging, have subcellular fraction resolution capability and biochemistry is special
The multiphoton microscope of different in nature sign ability then all has greatly improved from resolution, imaging depth etc., by biological tissue
Difference in optical property is imaged, and can reflect tissue physiology's situation and metabolic characteristicss, and have to human zero damage, resolution
Height, can dynamically, microscopically follow the tracks of physiology, the pathological change process of biological tissue.
The Chinese invention patent of Application No. 201410038761.6 discloses a kind of polarization frequency domain based on simple detector
Optical coherence tomography system, including low-coherence light source, first collimator, the polarizer, Michelson's interferometer, polarization beam splitting
Device, the second collimator, the first optical fiber, the 3rd collimator, the 4th collimator, the second optical fiber, the 5th collimator, grating, cylinder are saturating
Mirror, detector；The light that low-coherence light source sends sequentially passes through first collimator, is changed into polarized light after the polarizer and is coupled into Michael
The beam splitter of inferior interferometer, light beam is divided into reference arm light path and sample arm light path by this beam splitter, and the light of reference arm is through four points
One of be radiated on reference mirror after wave plate, the light of sample arm is gathered by image-forming objective lens through 2-D vibration mirror after quarter-wave plate
The burnt light with testing sample information in testing sample, being reflected back from testing sample and the reference light being reflected back from reference mirror
Interfere at described beam splitter；The outfan of Michelson's interferometer connects polarization beam apparatus, and this polarization beam apparatus will
Interfering beam is divided into horizontal polarization light and orthogonal polarized light two-way light, and horizontal polarization light is coupled into the first light by the second collimator
In fibre, incide on described grating the other end of the first optical fiber is parallel after the 3rd collimator, described orthogonal polarized light
Be coupled in the second optical fiber by the 4th collimator, the second optical fiber the other end after the 5th collimator parallel incide described
Grating on, described horizontal polarization light is different with the position that orthogonal polarized light incides grating, after grating beam splitting simultaneously by
Described cylindrical lenses are imaged in the zones of different of same detector, and detector diverse location is concurrently detected horizontal polarization letter
Number and vertical polarization signal, this two paths of signals is admitted to computer and carries out data processing after image pick-up card collection, obtains sample
The longitudinal intensity image of product, delayed image and quick shaft direction image.Additionally, the method is due to using simple detector, detector exists
Obtain and need when horizontal polarization and vertical polarization signal to wait in turn using, it detects and is unable to reach in real time.
The Chinese invention patent of Application No. 201210120196.9 discloses a kind of the many of biological tissue endogenous component
Pattern multi-photon microscopic imaging device, can carry out comprehensive, high contrast to the micro structure of biological tissue's endogenouss heterogeneity
The imaging of degree；Femtosecond laser is divided into two-way by spectroscope by the femtosecond laser being produced by femto-second laser, and the wherein first via flies
Second laser incides the exciting light producing wavelength ratio first via femtosecond laser length after optical parametric oscillator as pump light；Wherein
Second road femtosecond laser passes through reflecting mirror and delay line, and the exciting light producing with first via femtosecond laser is realized synchronously converging, with
Formation converges laser, and this converges laser and incides on scanning device by the first double-tone spectroscope, then passes through the second bi-color branch again
Light microscopic is simultaneously focused in detection sample by object lens, converges 2PEF, 3PEF, SHG and THG optical signal that laser is produced with detection sample
Collect back through object lens, then detection system is reflexed to by the second double-tone spectroscope, described detection system is included for reflecting
The long pass filter piece of 2PEF, 3PEF, SHG and THG optical signal and being separately positioned on the reflection light direction of those long pass filter pieces
Photomultiplier tube, 2PEF, 3PEF, SHG and THG optical signal is converted into the signal of telecommunication by described photomultiplier tube respectively, described electricity
Signal is respectively connected to the input of computer, is shown from biological tissue's endogenouss heterogeneity micro structure by computer simultaneously
High-contrast image, converge the two-photon fluorescence excitation that laser and detection sample produce, three-photon laser fluorescence, second harmonic and
Triple-frequency harmonics optical signal is collected back through identical object lens, directly reflexes to spy by the second double-tone spectroscope without scanning device
Examining system, it is to avoid because scanning device affects three-photon laser fluorescence and triple-frequency harmonics optical signal to the decay producing optical signal
Detect, and, carry out two kinds of signals of telecommunication that two photomultiplier tubes of second harmonic optical signals of self-organizing are converted into, be respectively connected to
The input of computer, the superposition in computer will improve the brightness of tissue Second Harmonic Imaging.The method is from laterally side
Face obtains the ultrastructure of the biological tissue with birefringence.
It is essentially all to biological tissue at present individually with optical coherent chromatographic imaging and multi-photon micro imaging system
It is imaged, single can only be obtained vertical or horizontal information it is impossible to difference is done to same pathological tissue the stressing of real-time synchronization
The analysis of point is so that cannot Overall Acquisition information during the diagnosis of pathology and monitoring treatment.Additionally, having very two-fold by force
Penetrate the biological tissue of property, its pathological changes has apparent variation characteristic on birefringence, be less frequently utilized the double of biology at present
Refrangibility is as the foundation of pathological diagnosis.
Content of the invention
It is an object of the invention to overcoming drawbacks described above, provide a kind of achievable synchronous acquisition biological tissue, particularly
There is the vertical structure of tissue of birefringence effect and the lossless, real-time, dynamically inclined of transversary, directivity and spectral signature
The optical coherence tomography that the shakes double-mode imaging system micro- with polarization multi-photon.In order to solve above-mentioned technical problem, the present invention adopts
Technical scheme be：
A kind of polarization optics coherent tomographic double-mode imaging system micro- with polarization multi-photon, including control and process mould
Block, polarization optics coherence chromatographic imaging module, polarization multi-photon micro-imaging module and scan module；
Described scan module includes main dichroscope, scanning galvanometer and numerical control three-dimensional sample platform；
Described polarization optics coherence chromatographic imaging module includes first laser device, isolator, the first polariser, polarization maintaining optical fibre
Bonder, the first quarter-wave plate, first collimator, plane mirror, the second quarter-wave plate, the second collimator, first anti-
Penetrate mirror, lens, polarising beam splitter, the first spectrogrph, the second spectrogrph, data collecting card；The light beam that first laser device sends
It is changed into polarized light after sequentially passing through isolator and the first polariser and be coupled into polarization-maintaining fiber coupler, will through polarization-maintaining fiber coupler
Light beam is divided into reference arm light path and sample arm light path, and the light of reference arm light path is through the first quarter-wave plate and first collimator
Reflect along backtracking to polarization-maintaining fiber coupler by plane mirror, the light of sample arm light path sequentially passes through the second quarter-wave
Piece, the second collimator and the first reflecting mirror, are reflected into scanning galvanometer at main dichroscope after lens focuss, then incide
On testing sample on digital control three-dimensional sample stage, from testing sample return light and from reference arm light path through plane mirror along former road
After the reference light returning is relevant at polarization-maintaining fiber coupler by polarization beam apparatus be divided into two-way respectively enter the first spectrogrph and
Second spectrogrph, data collecting card gathers the optical data on the first spectrogrph and the second spectrogrph；
Described polarization multi-photon micro-imaging module includes second laser, the second polariser, acousto-optic modulator, secondary two colors
Mirror, the second reflecting mirror, optical filter and 32 channel detectors；The light beam of second laser transmitting sequentially passes through the second polariser
And acousto-optic modulator, then incide on scanning galvanometer after time dichroscope and main dichroscope transmission successively, then incide number
On testing sample on control three-dimensional sample platform, reversely pass through main two colors from the fluorescence of testing sample return and second harmonic signal light
Reflect at secondary dichroscope after mirror, then reflex on optical filter by the second reflecting mirror, after filtered optical filtering, enter 30 two-ways
Road detector；
Described control is used for controlling scanning galvanometer and the working condition of numerical control three-dimensional sample platform with processing module, and is used for
The optical data of processing data capture card collection and the detection data of 32 channel detectors, and by the blending algorithm of image
Realize double-mode imaging.
The beneficial effects of the present invention is：By two kinds of different work(of polarization optics coherence chromatographic imaging and multi-photon micro-imaging
The image technology of energy integrates, and reaches two kinds of image mode Parallel applications；Using Optical Coherence Tomography Imaging Technology and light with
The second harmonic signal of matter interaction transmitting and fluorescence signal, obtain microstructure, the light of biological tissue at same position
The features such as spectrum, optical characteristics, and utilize its polarization characteristic, can effectively obtain the property of the biological tissue with birefringent characteristic
Matter, to realize the superimposed image of three-dimensional and pathology, spectrum, the birefringence of biological tissue；Special from longitudinal Yu horizontal bimodulus
Levy the pathological characteristics evaluating tissue, be a kind of real-time, lossless, dynamic imaging system.
Brief description
Fig. 1 is the principle of the embodiment of the present invention polarization optics coherent tomographic double-mode imaging system micro- with polarization multi-photon
Figure.
Label declaration：
1- polarization optics coherence chromatographic imaging module；2- polarizes multi-photon micro-imaging module；
3- scan module；4- controls and processing module；101- first laser device；102- isolator；
103- first polariser；104- polarization-maintaining fiber coupler；105- first quarter-wave plate；
106- first collimator；107- plane mirror；108- second quarter-wave plate；109- second collimator；
110- first reflecting mirror；111- lens；112- polarising beam splitter；113- first spectrogrph；
114- second spectrogrph；115- data collecting card；21- second laser；22- second polariser；
23- acousto-optic modulator；24- dichroscope；25- second reflecting mirror；26- optical filter；
27- 32 channel detector；The main dichroscope of 31-；32- scanning galvanometer；33- digital control three-dimensional sample stage.
Specific embodiment
By the technology contents of the detailed description present invention, structural features, realized purpose and effect, below in conjunction with embodiment
And coordinate accompanying drawing to be explained in detail.
The design of most critical of the present invention is：Polarization optics coherence chromatographic imaging is by ordinary optical coherence tomography system
Increase polarization mode, polarization multi-photon micro imaging system optionally increases polariser combination and forms polarization optics phase dried layer
Analysis imaging-multi-photon micro imaging system, the property using polarisation combines Optical Coherence Tomography Imaging Technology and light and material phase
The second harmonic signal of interaction transmitting carries out micro- structure imaging and analysis with fluorescence signal to having birefringent material,
Bimodulus image is obtained by the fusion of image, for detecting or diagnosing distribution and the pathological changes of the biological tissue with birefringence
Situation.
Refer to shown in Fig. 1, the polarization optics coherent tomographic of the present embodiment double-mode imaging system micro- with polarization multi-photon
System, include controlling with processing module 4, polarization optics coherence chromatographic imaging module 1, polarize multi-photon micro-imaging module 2 and sweeping
Retouch module 3；
Described scan module 3 includes main dichroscope 31, scanning galvanometer 32 and three-dimensional sample control platform 33；
Described polarization optics coherence chromatographic imaging module 1 includes first laser device 101, isolator 102, the first polariser
103rd, polarization-maintaining fiber coupler 104, the first quarter-wave plate 105, first collimator 106, plane mirror the 107, the 2nd 4/
One wave plate 108, the second collimator 109, the first reflecting mirror 110, lens 111, polarising beam splitter 112, the first spectrogrph 113,
Second spectrogrph 114, data collecting card 115；It is inclined that the light beam that first laser device 101 sends sequentially passes through isolator 102 and first
Shake and be changed into polarized light after device 103 and be coupled into polarization-maintaining fiber coupler 104, through polarization-maintaining fiber coupler 104, light beam is divided into reference
Arm light path and sample arm light path, the light of reference arm light path is after the first quarter-wave plate 105 and first collimator 106 through flat
Face mirror 107 reflects along backtracking to polarization-maintaining fiber coupler 104, and the light of sample arm light path sequentially passes through the second quarter-wave
Piece 108, the second collimator 109, the first reflecting mirror 110, are reflected into scanning at main dichroscope 31 after focusing on through lens 111 and shake
Mirror 32, then incides on the testing sample on digital control three-dimensional sample stage 33, from the light of testing sample return with from reference arm light
Lu Shangjing plane mirror 107 along backtracking reference light at polarization-maintaining fiber coupler 104 relevant after by 112 points of polarization beam apparatus
Two-way is become to respectively enter the first spectrogrph 113 and the second spectrogrph 114, data collecting card 115 gathers the first spectrogrph 113 and the
Optical data on two spectrogrphs 114；
Described polarization multi-photon micro-imaging module 2 includes second laser 21, the second polariser 22, acousto-optic modulator
23rd, secondary dichroscope 24, the second reflecting mirror 25, optical filter 26 and 32 channel detectors 27；The light of second laser 21 transmitting
Bundle sequentially passes through the second polariser 22 and acousto-optic modulator 23, then enters after time dichroscope 24 and main dichroscope 31 transmission successively
It is mapped on scanning galvanometer 32, then incide on the testing sample on digital control three-dimensional sample stage 33, glimmering from testing sample return
Light and second harmonic signal light reversely pass through reflection at secondary dichroscope 24 after main dichroscope 31, then are reflected by the second reflecting mirror 25
To on optical filter 26, after filtered 26 optical filtering, enter 32 channel detectors 27；
Described control is used for controlling scanning galvanometer 32 and the working condition of numerical control three-dimensional sample platform 33 with processing module 4, with
And the detection data of the optical data for processing data capture card 115 collection and 32 channel detectors 27, and by figure
The blending algorithm of picture realizes double-mode imaging.
The work process of the present invention is：The light beam that first laser device 101 sends sequentially passes through isolator 102 and the first polarization
It is changed into polarized light after device 103 and is coupled into polarization-maintaining fiber coupler 104, through polarization-maintaining fiber coupler 104, light beam is divided into reference arm
Light path and sample arm light path, the light of reference arm light path is after the first quarter-wave plate 105 and first collimator 106 through plane
Mirror 107 reflects along backtracking to polarization-maintaining fiber coupler 104, and the light of sample arm light path sequentially passes through the second quarter-wave plate
108th, the second collimator 109, the first reflecting mirror 110, is reflected into scanning galvanometer after focusing on through lens 111 at main dichroscope 31
32, then incide on the testing sample on digital control three-dimensional sample stage 33, from the light of testing sample return with from reference arm light path
On through plane mirror 107 along backtracking reference light at polarization-maintaining fiber coupler 104 relevant after be divided into by polarization beam apparatus 112
Two-way respectively enters the first spectrogrph 113 and the second spectrogrph 114, and data collecting card 115 gathers the first spectrogrph 113 and second
Optical data on spectrogrph 114；The light beam of second laser 21 transmitting sequentially passes through the second polariser 22 and acousto-optic modulator
23, then incide on scanning galvanometer 32 after time dichroscope 24 and main dichroscope 31 transmission successively, then incide numerical control three
On testing sample on dimension sample stage 33, reversely pass through main dichroscope from the fluorescence of testing sample return and second harmonic signal light
Reflect at secondary dichroscope 24 after 31, then reflex on optical filter 26 by the second reflecting mirror 25, enter after filtered 26 optical filtering
32 channel detectors 27；The fluorescence that the optical data of data collecting card 115 collection, 32 channel detectors 27 detect
Pass through to control and processing module 4 with second harmonic light simultaneously, be analyzed processing with processing module 4 controlling, at same position
The horizontal and vertical image of the features such as the microstructure of acquisition biological tissue, spectrum, optical characteristics, is calculated by the fusion of image
Method obtains bimodulus image, and inversion of three dimensional information, realizes superimposed image and pathology, spectrum, the birefringence of the three-dimensional of biological tissue
Property.
Knowable to foregoing description, the beneficial effects of the present invention is：Polarization optics coherence chromatographic imaging and multi-photon are shown
The image technology of two kinds of difference in functionalitys of micro- imaging integrates, and reaches two kinds of image mode Parallel applications；Using Optical coherence tomography
The second harmonic signal of analysis imaging technique and light-matter interaction transmitting and fluorescence signal, obtain biological group at same position
The features such as the microstructure knitted, spectrum, optical characteristics, and utilize its polarization characteristic, can effectively obtain with birefringent characteristic
Biological tissue property, to realize the superimposed image of three-dimensional and pathology, spectrum, the birefringence of biological tissue；From longitudinal direction
With the pathological characteristics of horizontal bimodulus characteristic evaluating tissue, it is that one kind is quickly real-time, lossless, dynamic double-mode imaging system.
Further, described first laser device 101 is wideband light source or frequency swept laser.
Specifically, the wavelength of wideband light source or frequency swept laser is 1310nm.
Seen from the above description, there is output high, swept frequency range width, enable high-resolution.
Further, described first spectrogrph 113 and the second spectrogrph 114 separately detect horizontal and vertical polarization direction
Information.
Seen from the above description, separately detect the information of horizontal and vertical polarization direction by two spectrogrphs, obtain water
The information of gentle vertical polarization light.
Further, described polarization-maintaining fiber coupler 104 is to have 50:2 × 2 bonders of 50 splitting ratios.
Seen from the above description, line polarized light coupling and light splitting are realized.
Further, described second laser 21 is ti sapphire laser.
Specifically, the wavelength regulation scope of ti sapphire laser is 700nm-980nm.
Seen from the above description, it can be avoided that the phase mutual respect of second harmonic light in the biological tissue and fluorescence signal
Folded, thus obtaining high-contrast image.
Further, described main dichroscope 31 is more than the light of 1000nm for reflection wavelength and transmission peak wavelength is less than 1000nm's
The main dichroscope of light, described dichroscope 24 is less than the light of 700nm for reflection wavelength and transmission peak wavelength be more than 700nm light time
Dichroscope.
Seen from the above description, the fluorescence being returned due to polarization multi-photon micro-imaging module and the light source of second harmonic
Wavelength is less than excitation source, and the wavelength of fluorescence is slightly below excitation wavelength, is between 420nm-700nm, the center of second harmonic light
Wavelength is the half of excitation source, is between 350nm-490nm；Main dichroscope is mainly polarization optics coherence chromatographic imaging
Light is made a distinction with the light of polarization multi-photon micro-imaging, and secondary dichroscope is the excitation source of polarization multi-photon micro imaging system
Make a distinction with the light of fluorescence, second harmonic.
Further, the blending algorithm of described image is the image co-registration of pyramid decomposition blending algorithm and wavelet transformation
Algorithm.
Seen from the above description, by the Image Fusion of pyramid decomposition blending algorithm and wavelet transformation, obtain high
The double-mode imaging image of picture quality.
Further, described digital control three-dimensional sample stage 33 is the electronic control translation stage of three axle electric precision.
Seen from the above description, the electronic control translation stage of three axle electric precision drives the sample stage placing sample to do two dimension and sweeps
Retouch, reach the effect of 3-D scanning.
Refer to shown in Fig. 1, embodiments of the invention one are：
Using Skin scar as sample, because collagen fiber have very strong birefringence, its polarization characteristic can be very
Show well the feature that collagen damages.
A kind of polarization optics coherent tomographic double-mode imaging system micro- with polarization multi-photon, including control and processing module
4th, polarization optics coherence chromatographic imaging module 1, polarization multi-photon micro-imaging module 2 and scan module 3；Described scan module 3
Including main dichroscope 31, scanning galvanometer 32 and numerical control three-dimensional sample platform 33；In polarization optics coherence chromatographic imaging module 1, with
As light source, the light beam that swept light source 101 sends sequentially passes through isolator 102 and the first polarization to the swept light source 101 of 1310nm
It is changed into polarized light after device 103 and be coupled into 50:The polarization-maintaining fiber coupler 104 of the 2 × 2 of 50 splitting ratios, through polarization-maintaining fiber coupler
Light beam is divided into two-way light by 104, i.e. reference arm light path and sample arm light path；The light of reference arm light path is derived by optical fiber, Jing Guo
Reflect along backtracking to polarization-maintaining fiber coupler through plane mirror 107 after one quarter-wave plate 105 and first collimator 106
104, the light of sample arm light path sequentially pass through the second quarter-wave plate 108, the second collimator 109, after the first reflecting mirror 110,
It is reflected into scanning galvanometer 32 at main dichroscope 31 after focusing on through lens 111, then incide on digital control three-dimensional sample stage 33
Testing sample on；Meanwhile polarization multi-photon micro-imaging module 2 works, with the Ti∶Sapphire laser tunable laser of 850nm
As second laser 21, the light beam of ti sapphire laser 21 transmitting sequentially passes through the second polariser 22 and acousto-optic modulator 23,
Then incide on scanning galvanometer 32 after the transmission through time dichroscope 24 and main dichroscope 31 successively, secondary dichroscope 24 reflection wavelength
Light less than the 700nm and transmission peak wavelength light more than 700nm, main dichroscope 31 reflection wavelength is more than light and the transmitted wave of 1000nm
The long light less than 1000nm, then incides on the testing sample on digital control three-dimensional sample stage 33；Control and control with processing module 4
Scanning galvanometer 32, to determine sweep limitss, controls digital control three-dimensional sample stage 33 to realize to sample 3-D scanning simultaneously.Numerical control three
Testing sample on dimension sample stage 33 is made through polarization optics coherence chromatographic imaging module 1 and polarization multi-photon micro-imaging module 2
Produce three kinds of light with rear, respectively：The light of polarization optics coherence chromatographic imaging module 1 enters the reflected light of sample, Ti∶Sapphire laser swashs
The fluorescence that the second harmonic light of light and the generation of birefringent collagen medium interaction collagen, elastic fiberss produce, wherein polarizes
In optical coherent chromatographic imaging module 1, from testing sample return light and from reference arm light path through plane mirror 107 along former road
It is divided into two-way by polarization beam apparatus 112 after the reference light returning is relevant at polarization-maintaining fiber coupler 104 and respectively enter the first light
Spectrometer 113 and the second spectrogrph 114, gather on the first spectrogrph 113 and the second spectrogrph 114 through data collecting card 115
Optical data, the optical data that data collecting card 115 is collected delivers to control and processing module 4；Ti sapphire laser 21
The light beam penetrated reversely is passed through after main dichroscope 31 in secondary dichroscope 24 with the fluorescence of testing sample generation and second harmonic signal light
Place's reflection, then reflex on optical filter 26 by the second reflecting mirror 25, it is true that optical filter 26 reaches type according to concrete acquisition optical signal
Surely using the number of optical filter, at most can reach 32 passages, after filtered 26 optical filtering, enter 32 channel detections
Device 27；Optical filter 26 is one group of filter set, and cooperation 32 channel detector 27 uses, due to polarizing the micro- one-tenth of multi-photon
The fluorescence returning as module and the wavelength of the light source of second harmonic are less than excitation source, and the wavelength of fluorescence is slightly below excitation wavelength,
Between 420nm-700nm, the centre wavelength of second harmonic light is the half of excitation source, is between 350nm-490nm, therefore this
In case, optical filter 26 arranges two and receives second harmonic light and fluorescence respectively, and the optical filter 26 receiving second harmonic is set to ripple
The light of long 405nm-445nm passes through, and the optical filter 26 receiving fluorescence is set to the light of wavelength 450nm-650nm and passes through, and respectively
By two channel reception spectral informations in 32 channel detectors 27, the data of acquisition, by controlling and processing module 4, is passed through
Image Fusion shows second harmonic intensity in control with processing module 4, can obtain the directivity of sample simultaneously；And with
Two polarization states of polarization optics coherent tomographic module 1 merge, with obtain the structural information of same range of information, spectral information and
Optical property parameter；By the image co-registration of pyramid decomposition blending algorithm and the Image Fusion of wavelet transformation, obtain partially
The optical coherence tomography that the shakes double-mode imaging image micro- with polarization multi-photon.The method can quickly, live body, detect in real time
The situation of change in biological tissue collagen direction under pathological changes or photothermal injury.
In sum, the polarization optics coherent tomographic that the present invention the provides double-mode imaging system micro- with polarization multi-photon,
The image technology of two kinds of difference in functionalitys of polarization optics coherence chromatographic imaging and multi-photon micro-imaging is integrated, reaches two kinds
Image mode Parallel application；Second harmonic signal using Optical Coherence Tomography Imaging Technology and light-matter interaction transmitting
With fluorescence signal, the horizontal information at the same position of biological tissue and longitudinal information can be obtained simultaneously, obtain preferably imaging and differentiate
Rate and deeper imaging depth, in conjunction with its polarization characteristic, obtain the microstructure of Ultrastructural disdiaclast, are had
There are the high-resolution of the material of birefringence effect, the image of high-contrast and spectrum, its bimodulus can be obtained by the fusion of image
Image, can obtain the spectral signature of its feature, intensity and direction and material by analysis, realize the three-dimensional of biological tissue simultaneously
Superimposed image and pathology, spectrum, birefringence；Its resolution can reach 10nm, and imaging depth can reach 1-3mm；From longitudinal direction
With the pathological characteristics of horizontal bimodulus characteristic evaluating tissue, it is that one kind is quickly real-time, lossless, dynamic double-mode imaging system, is
Its physiology of the qualitative detection such as the biological tissues such as tumor cell, pathological change process provide facility.
The foregoing is only embodiments of the invention, not thereby limit the present invention the scope of the claims, every using this
Equivalent structure or equivalent flow conversion that bright description and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (10)
1. a kind of polarization optics coherent tomographic with the polarization micro- double-mode imaging system of multi-photon it is characterised in that：Including control
With processing module, polarization optics coherence chromatographic imaging module, polarization multi-photon micro-imaging module and scan module；
Described scan module includes main dichroscope, scanning galvanometer and numerical control three-dimensional sample platform；
Described polarization optics coherence chromatographic imaging module includes first laser device, isolator, the first polariser, polarization maintaining optical fibre coupling
Device, the first quarter-wave plate, first collimator, plane mirror, the second quarter-wave plate, the second collimator, the first reflecting mirror,
Lens, polarising beam splitter, the first spectrogrph, the second spectrogrph, data collecting card；The light beam that first laser device sends warp successively
It is changed into polarized light after crossing isolator and the first polariser and is coupled into polarization-maintaining fiber coupler, through polarization-maintaining fiber coupler, light beam is divided
For reference arm light path and sample arm light path, the light of reference arm light path is after the first quarter-wave plate and first collimator through flat
The reflection of face mirror along backtracking to polarization-maintaining fiber coupler, the light of sample arm light path sequentially pass through the second quarter-wave plate, the
Two collimators and the first reflecting mirror, are reflected into scanning galvanometer at main dichroscope after lens focuss, then incide numerical control
On testing sample on three-dimensional sample platform, from testing sample return light and from reference arm light path through plane mirror along backtracking
Reference light relevant at polarization-maintaining fiber coupler after be divided into two-way by polarization beam apparatus and respectively enter the first spectrogrph and second
Spectrogrph, data collecting card gathers the optical data on the first spectrogrph and the second spectrogrph；
Described polarization multi-photon micro-imaging module include second laser, the second polariser, acousto-optic modulator, secondary dichroscope,
Second reflecting mirror, optical filter and 32 channel detectors；Second laser transmitting light beam sequentially pass through the second polariser and
Acousto-optic modulator, then incides on scanning galvanometer after time dichroscope and main dichroscope transmission successively, then incides numerical control
On testing sample on three-dimensional sample platform, reversely pass through main dichroscope from the fluorescence of testing sample return and second harmonic signal light
Reflect at secondary dichroscope afterwards, then reflex on optical filter by the second reflecting mirror, after filtered optical filtering, enter 32 passages
Detector；
Described control is used for controlling scanning galvanometer and the working condition of numerical control three-dimensional sample platform with processing module, and is used for processing
The optical data of data collecting card collection and the detection data of 32 channel detectors, and realized by the blending algorithm of image
Double-mode imaging.
2. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described first laser device is wideband light source or frequency swept laser.
3. the polarization optics coherent tomographic according to claim 2 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：The wavelength of wideband light source or frequency swept laser is 1310nm.
4. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described first spectrogrph and the second spectrogrph separately detect the information of horizontal and vertical polarization direction.
5. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described polarization-maintaining fiber coupler is to have 50:2 × 2 bonders of 50 splitting ratios.
6. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described second laser is ti sapphire laser.
7. the polarization optics coherent tomographic according to claim 6 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：The wavelength regulation scope of described ti sapphire laser is 700nm-980nm.
8. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described main dichroscope is more than the light of 1000nm for reflection wavelength and transmission peak wavelength be less than 1000nm light master two color
Mirror, described dichroscope is less than the light of 700nm for reflection wavelength and transmission peak wavelength be more than 700nm light secondary dichroscope.
9. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：Described digital control three-dimensional sample stage is the electronic control translation stage of three axle electric precision.
10. the polarization optics coherent tomographic according to claim 1 double-mode imaging system micro- with polarization multi-photon, it is special
Levy and be：The blending algorithm of described image is the Image Fusion of pyramid decomposition blending algorithm and wavelet transformation.
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