CN106381253A - Process for nutritional enhancing production of cordyceps militaris yellow rice wine by cordyceps militaris rice culture material - Google Patents
Process for nutritional enhancing production of cordyceps militaris yellow rice wine by cordyceps militaris rice culture material Download PDFInfo
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Abstract
The invention belongs to the technical field of yellow rice wine production and processing, and in particular, relates to a process for nutritional enhancing production of cordyceps militaris yellow rice wine by taking rice soaked by water and a cordyceps militaris liquid strain as a main material and adopting a rice cordyceps militaris culture material cultivated through inoculation, spawn running and coloring and inducement to primordium to participate in fermentation, wherein the process comprises the following steps: (A) making rice fine white; (B) dipping the rice; (C) steaming the rice; (D) making the rice drop into a tank and fermenting; (E) carrying out post-fermentation; (F) clarifying and squeezing; (G) frying wine; and (H) storing and aging, wherein the cordyceps militaris rice culture material is added to the step (C) of rice cooking, or the cordyceps militaris rice culture material is added to the step (D) of tank dropping and fermentation. The cordyceps militaris rice culture material is prepared by the following steps: (a) preparing a liquid culture medium; (b) preparing a rice compound culture medium; (c) inoculating; (d) carrying out spawn running; and (e) carrying out coloring and inducement to primordium. The produced cordyceps militaris yellow rice wine has effective components and nutritional value of cordyceps militaris and yellow rice wine and enriches the varieties of the yellow rice wine.
Description
Technical field
The invention belongs to rice wine production processing technique field, big specifically related to soaked with water, Cordyceps militaris strain
Rice is major ingredient, is cultivated the Cordyceps militaris (L.) Link. rice compost generating and is participated in fermentation, fortification production with inoculation, a bacterium, annesl flower bud
The technique of Cordyceps militaris (L.) Link. yellow wine.
Background technology
Yellow wine is one of big famous brand of wine in the world three, is also Chinese history wine kind the longest.Its alcoholic strength and suitable, vinosity is pure and sweet,
From ancient times to the present very popular, next is exhausted to can be rated as sky.Yellow wine is China's distinctive wine kind, is the national special product of China, enjoys
There is the good reputation of national wine.Yellow wine with rice, Semen Panici miliacei, fructus zizaniae caduciflorae, Semen Maydiss, Semen Tritici aestivi etc. as raw material, through steaming, mix with malt yeast, rice-koji or
Yeast for brewing rice wine, carries out saccharifying and fermentation brew forms.Yellow wine alcoholic strength 14% to 20%, containing abundant nutrient substance, including 21 kinds
Aminoacid, wherein has unknown aminoacid and 8 kinds of essential amino acids, its content occupies first of various brewed wines, than medicated beer, wine
Exceed 2~36 times with sake, be described as " liquid cake ".
Cordyceps militaris (L.) Link. contains cordycepin, cordycepic acid, Cordyceps polysaccharide, superoxide dismutase (SOD), selenium (Se) etc., and it is main
Chemical composition and health care are much like with natural cordyceps, have antiasthmatic-antitussive, strengthening yang and invigorating kidney function, to emphysema, trachea
Inflammation has good therapeutic effect, play the role of substantially to reduce blood glucose, glucose in urine, reduce blood pressure, cranium pressure;Also have antibacterial, anticancer, strengthen immune work(
The effect such as energy, not yet finds obvious side effect, its health care is increasingly recognized so far.With with rice as culture medium
Cultivate the increasingly mature of Cordyceps militaris (L.) Link. technology, the processing of Cordyceps militaris (L.) Link. yellow wine has had been provided with good basic condition.
Through rice dipping, steamed rice, the cylinder that falls, heat-preservation fermentation disclosed in Application No. 201210302128.4, add Cordyceps militaris (L.) Link./arhat
The yellow wine that after really/Fructus Lycii, ferment, squeezing, sterilisation step are produced, is that the effective ingredient in Cordyceps militaris (L.) Link.sporophore (finished product) is used
Yellow wine is extracted, and the cycle is long, high cost.Through rice dipping, steamed rice, the meal that dries in the air, addition disclosed in Application No. 201310058520.3
The Huang that Cordyceps militaris (L.) Link. fermented supernatant fluid (the fermentation substantially stops) saccharifying of sterilizing, alcohol fermentation, allotment, sterilization steps are produced
Wine, the Active Components in Various Cordyceps militaris Strains in Cordyceps militaris (L.) Link. fermented supernatant fluid is not strong.The applicant exists《Ankang University's journal》The paper delivered
《Safe and comfortable selenium-enriched cordceps militaris yellow wine production technology is inquired into》It is with safe and comfortable local Semen oryzae sativae rich in selenium and water as raw material, there is certain ground
Domain limitation, in addition yellow wine disclosed in this paper there is pungent highly seasoned, the problem that fragrance is not sufficiently stable.In addition, on the market
Yellow wine designs and varieties little.
Content of the invention
For solving above-mentioned technical problem, the invention discloses a kind of Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link.
The technique of yellow wine, comprises the following steps:
(A) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white;
(B) rice dipping:Pure white rice 95-105kg, first with water by meter Chong Xi 1 time, drains away the water, is placed in tank, add rice
Amount 2%-4% Cordyceps militaris strain, the 117-120kg that adds water covers rice and is soaked, soak time 1-3 days, water absorption
25%-30% so that the grain of rice complete and crisp for degree, rice dipping terminates;
(C) steamed rice:After rice dipping terminates, drain rice, add Cordyceps militaris (L.) Link. rice compost 25-28kg, then Cordyceps militaris (L.) Link. will be added
Pot atmospheric cooking 25-30 minute on the rice of rice compost, rice reaches outer firmly interior soft, the interior no raw heart, loose does not paste, thoroughly and not
Rotten, uniformity, steamed rice completes;
(D) tank that falls ferments:The rice cooking falls in fermentation tank by air-cooled or water-cooled, the 230-240kg that adds water in proportion,
Malt yeast 13-16kg mix homogeneously, plus lactic acid 0.25-0.8kg regulation PH to 4, when temperature drop is to 26~30 DEG C, add yeast wine
4-6kg, when temperature is raised to 31~32 DEG C, opens rake stirring, keeps the temperature at 28~30 DEG C, the tank that falls is fermented into wine within 2~3 days
Wine with dregs;
(E) after fermentation:The swort of fermentation tank is moved into after fermentation tank, controls product temperature and room temperature at 15~18 DEG C, static
Ferment 20~30 days;
(F) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clarified at low temperature
2~3 days, finally the wine liquid supernatant is filtered through cotton cake filtering machine;
(G) decoct wine:Carry out decocting wine by filtering the wine completing, temperature is 85~90 DEG C, time 15mins, decoct after drinking, by heat
Wine pours in the empty altar of sterilization;
(H) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis or indocalamus shell after pouring into the empty altar of sterilization, then uses mud head
Or warehouse-in storage after Gypsum Fibrosum sealing, warehouse-in aging 1 year, obtain wine degree more than 16%, acidity, below 0.45, has rich in Cordyceps militaris (L.) Link.
The yellow wine of effect composition;
(C) the Cordyceps militaris (L.) Link. rice compost described in steamed rice step is prepared from by following steps:
A () prepares fluid medium:Weigh natural nitrogen source dried silkworm chrysalis meal 13-16, glucose 8-10, peptone 1- in mass ratio
3rd, potassium dihydrogen phosphate 1-2, magnesium sulfate 0.5-1, ammonium citrate 0.5-1, VB10.1-0.3, water 1000, stir, are distributed into
500ml conical flask, absorbent cotton seals, and 121-126 DEG C of autoclaving 30mins is cooled to less than 30 DEG C standby, fluid mediums
Preparation completes;
B () prepares rice complex medium:Take clean culture bottle, in culture bottle, first fill 28-32g rice, add liquid
Culture medium 33-36ml, polyethylene film seals, 121-126 DEG C of autoclaving 30mins or 100 DEG C of sterilizing 8h, is cooled to 30 DEG C
Standby below, rice complex medium is prepared and is completed;
C () is inoculated:In the inoculating hood of sterilizing, operation by alcohol burner, draw 5ml Cordyceps militaris with asepsis injector
Strain, is uniformly injected in the culture bottle equipped with rice complex medium, covers sealed membrane or bottle cap immediately, inoculation completes;
D () sends out bacterium:The culture bottle that will be equipped with the rice complex medium inoculating Cordyceps militaris strain is taken sterilized
Incubator or culturing room cultivated, under dark condition, keeping temperature 16-18 DEG C, humidity 60-65%, 13-15 days, bottle
Interior white hypha is dense luxuriant, covers with rice complex medium, sends out bacterium and completes;
(e) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime pair
It carries out photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 little
When, temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the training of Cordyceps militaris (L.) Link. rice
Nutriment.
Now, to contain Active Components in Various Cordyceps militaris Strains the strongest for rice compost, is suitable to produce selenium-enriched cordceps militaris yellow wine.
Its further technical scheme is:
Cordyceps militaris (L.) Link. rice compost is substituted for by addition in (C) steamed rice step in falling tank fermentation step at (D) and adds.
Its further technical scheme is:
Meter Xuan Yong content of starch is high, the soft rice of absorb water fast, easy gelatinizing and saccharifying.
Its further technical scheme is:
Water adopts purified phreatic water or spring water.
Its further technical scheme is:
(G) decoct in wine step and wine is decocted using tubular heat exchanger.
Its further technical scheme is:
(H) in storage aging step, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature 5-20 DEG C,
Turning every year 1-2 time.
In the present invention, the pure white of (A) rice, is to contain protein and fat is more due to the outer skin of rice, simultaneously also richness
There is the nutritional labeling of the microorganisms such as ash and vitamin, easily cause the breeding of raw acid bacterium so that the acidity of wine is increased, by pure white,
Can solve the problems, such as that yellow wine acidity is too high.
(B) rice dipping purpose is to make starch imbibition, is easy to steaming and decocting gelatinizing, and soak time is according to temperature, water temperature and rice matter
Depending on.Add Cordyceps militaris strain during rice dipping, improve the content of effective ingredient in yellow wine.
(C) steamed rice purpose is to make starch gelatinization.
(E) after fermentation is it is therefore an objective to yeast ferments further, and improves the local flavor of wine.
(F) clarification squeezing, is because containing the materials such as starch, yeast, insoluble protein and a small amount of cellulose in yellow wine,
Clarifying treatment must be carried out for opposite wine at low temperature.
(G) decoct wine, be for the activity killing the microorganism in wine liquid and destroy remaining enzyme, remove the miscellaneous taste of raw wine, make egg
White matter isocolloid mass sets precipitate, and stable to guarantee yellow rice wine quality, through decocting after wine process, the color and luster of yellow wine becomes bright.
Agents useful for same of the present invention and raw material are all commercially available.
The raw material of Cordyceps militaris (L.) Link. rice compost of the present invention is natural nitrogen source dried silkworm chrysalis meal, glucose, VB1Piece, malt yeast, yeast wine are equal
For daily bread raw material, without any side effects to human body.
The present invention makes full use of various functions composition in Cordyceps militaris (L.) Link., improves the quality of traditional yellow rice wine, the pattern of abundant yellow wine
Kind, increases economic efficiency, and through test of many times, the present invention adopts the Cordyceps militaris (L.) Link. that Cordyceps militaris (L.) Link. rice compost fortification produces yellow
Wine, has that mellow in taste, color, smell and taste are all good, the function of antibacterial, anticancer, enhancing immunity.Cordyceps militaris (L.) Link. yellow wine utilizes high quality white rice
Inoculation Cordyceps militaris strain, the temperature of simulation Cordycepses growth, humidity, illumination, ventilation, the condition such as nutrition, through send out a bacterium, photosensitive turn
Color produces the Cordyceps militaris (L.) Link. rice compost rich in Cordyceps militaris (L.) Link. effective ingredient.By rice through pure white, use water and Cordyceps militaris strain
Rice dipping, steamed rice, the meal that dries in the air, the cylinder fermentation that falls, after fermentation, clarification squeezing, pan-fried wine, storage aging step simultaneously add pupa in steamed rice step
Cordycepses rice compost or addition Cordyceps militaris (L.) Link. rice compost participation fermentation in the cylinder fermentation step that falls, carry out fortification life
The Cordyceps militaris (L.) Link. yellow wine producing.Its Cordyceps militaris strain is from healthy and strong, yield high activity Cordyceps militaris (L.) Link., by separate tissue, purification,
It is seeded in liquid fermentation tank (bottle) and carries out fermentation 5-7 days, treat clearly haze-free in liquid-based, have the little mycelia fragment appearance to be
Become.The Cordyceps militaris (L.) Link. yellow wine that the present invention produces has effective ingredient and the nutritive value of Cordyceps militaris (L.) Link. and yellow wine concurrently, enriches the flower of yellow wine
Chromaticity kind.
The production technology of Cordyceps militaris (L.) Link. yellow wine of the present invention and rice wine production work disclosed in Application No. 201310058520.3
Skill is compared, and increased pure white operation, reduces yellow wine acidity further, adds Cordyceps militaris strain, improve yellow wine during rice dipping
The content of middle effective ingredient, Cordyceps militaris (L.) Link. rice compost participates in effective ingredient and the quality that fermentation further increases yellow wine;With
Yellow wine production technology disclosed in application number 201210302128.4 is compared, and increased pure white operation, solves yellow wine acidity too high
Problem, adds Cordyceps militaris strain during rice dipping, can improve the content of effective ingredient in yellow wine, with containing Cordyceps militaris (L.) Link. effective ingredient
Rice compost participate in yellow wine fermentation, so that the mouthfeel of Cordyceps militaris (L.) Link. yellow wine, quality is carried further than being directly added into Cordyceps militaris (L.) Link. finished product
Rise, the cycle is shorter, cost is lower;The paper delivered with applicant《Safe and comfortable selenium-enriched cordceps militaris yellow wine production technology is inquired into》Disclosed
Yellow wine production technology is compared, for improving the content of effective ingredient in yellow wine further it is considered to add liquid spawn, due to liquid bacteria
Plant and contain a large amount of mycelium, in thick, through test of many times, add Cordyceps militaris strain using during rice dipping, and determine
Good parameter area;Pan-fried wine temperature is brought up to 85~90 DEG C by original 70~75 DEG C, the pan-fried wine time was reduced to 15 by 20 minutes
Minute, the activity that both ensure that the microorganism killing in wine liquid and destroyed remaining enzyme, remove the miscellaneous taste of raw wine, make protein isocolloid
Mass sets precipitate, and stable to guarantee yellow rice wine quality, through decocting after wine process, the color and luster of yellow wine becomes bright, and greatly reduces
The loss of some aminoacid in yellow wine, these aminoacid are heat-sensitive substances, heat is easily decomposed, is polymerized, when reducing heating
Between these aminoacid can be made to retain as far as possible so that yellow wine nutrition is more rich, improve the quality of yellow wine;Reduce pupa for effective
Aweto yellow wine acid, its additive, through test of many times, have finally chosen lactic acid, and by lot of experiments it is thus identified that falling
In tank fermentation procedure, the associated production technological parameter of addition lactic acid and this tank fermentation procedure that falls is so that Cordyceps militaris (L.) Link. yellow wine acid subtracts
Few, vinosity is softer, mellow, and mouthfeel is good, fragrance is stable, more popular with consumers.
Beneficial effects of the present invention are as follows:
(1) add Cordyceps militaris strain during rice dipping of the present invention, improve the content of effective ingredient in yellow wine.
(2) present invention, with rice as major ingredient, cultivates, with inoculation, a bacterium, annesl flower bud, the Cordyceps militaris (L.) Link. rice compost generating
Participate in fermentation, carry out fortification and produce Cordyceps militaris (L.) Link. yellow wine, so that the quality of Cordyceps militaris (L.) Link. yellow wine is lifted further.
(3) increased natural nitrogen source dried silkworm chrysalis meal in fluid medium, decrease the application of the biogenetic products such as peptone, improve
The safety of product.
(4) add lactic acid in fermenting:Lactic acid is mutually merged with other organic acid, plays cushioning balance work to the taste of wine
With making vinosity be in harmonious proportion, reducing acid, bring good local flavor to yellow wine.And lactic acid has that viscosity is big, boiling point is high, Yi Ning
Solid feature, so the evaporation rate of scent molecule in wine can be changed, play mediation system taste, stablize the work(of vinosity fragrance
Energy.Lactic acid is the tie of flavor component and bridging agent in wine body, can effectively alleviate the ester-type hydrolysis in wine, promotes wine body steady
Fixed, it is simultaneously also beneficial to the aging of new wine, improves the fusion degree of each flavor substance in wine body, increase the compound of wine body note gas
Property, show the effect of the stable aging agent of lactic acid.
(5) decoct wine temperature and bring up to 85-90 DEG C, the time is reduced to 15mins, improve temperature, reduce the time, greatly reduce
The loss of beneficial heat-sensitive substance in finished wine.
(6) because the outer skin of rice contains protein and fat is more, also it is rich in micro- life such as ash and vitamin simultaneously
The nutritional labeling of thing, easily causes the breeding of raw acid bacterium so that the acidity of wine is increased, by pure white, can solve yellow wine acidity too high
Problem.
(7) present invention is not that effective ingredient to Cordyceps militaris (L.) Link.sporophore (finished product) is extracted with yellow wine, and ratio is directly added into
The Cordyceps militaris (L.) Link. finished product cycle is shorter, and cost is lower.
(8) present invention utilizes the intermediate Cordyceps militaris (L.) Link. rice compost that Cordyceps militaris (L.) Link. produces, and Active Components in Various Cordyceps militaris Strains is more
Enrichment.
Brief description
Fig. 1 is the process chart that a kind of Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine;
Fig. 2 is another process chart that a kind of Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine.
Specific embodiment
Below by specifically embodiment, the present invention is described further
Embodiment 1
As shown in figure 1, Cordyceps militaris (L.) Link. rice compost is prepared from by following steps:
(a1) prepare fluid medium:Natural in mass ratio nitrogen source dried silkworm chrysalis meal 15, glucose 10, peptone 3, biphosphate
Potassium 2, magnesium sulfate 1, ammonium citrate 1, VB10.2, the ratio of water 1000, the above material of precise, add water and stir, subpackage
Enter 500ml conical flask, absorbent cotton seals, 121-126 DEG C of autoclaving 30mins makes fluid medium;
(b1) prepare rice complex medium:Take clean culture bottle, in culture bottle, first fill 30g rice, add liquid training
Foster base 35ml, polyethylene film seal, 121-126 DEG C of autoclaving 30mins or 100 DEG C sterilizing 8h, be cooled to less than 30 DEG C standby
With rice complex medium is prepared and completed;
(c1) inoculate:In the inoculating hood of sterilizing, inoculated by the requirement of sterile working, by alcohol burner, operation, beats
Open sealed membrane or the bottle cap of culture bottle, draw 5ml Cordyceps militaris strain with asepsis injector, be uniformly injected into and be combined equipped with rice
In the culture bottle of culture medium, cover sealed membrane or bottle cap immediately, inoculation completes;
(d1) send out bacterium:Will be equipped with inoculate Cordyceps militaris strain rice complex medium culture bottle take sterilized
Incubator or culturing room are cultivated, and send out the bacterium stage under dark condition, 17 DEG C of keeping temperature, humidity 65%, through 15 days, in vain
Color mycelia can cover with rice complex medium, and mycelia is dense luxuriant, send out bacterium and complete;
(e1) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime pair
It carries out photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 little
When, temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the training of Cordyceps militaris (L.) Link. rice
Nutriment.
Now, to contain Active Components in Various Cordyceps militaris Strains the strongest for rice compost, is suitable to produce selenium-enriched cordceps militaris yellow wine.
The technique that this Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine comprises the following steps:
(A1) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white, meter Xuan Yong content of starch is high,
Fast, the easy gelatinizing of water suction and the soft rice of saccharifying;
(B1) rice dipping:Weigh the rice that 95kg is processed through pure white, first cleaned with water 1 time, drain away the water, be placed in tank, plus
Enter 2kg Cordyceps militaris strain, the 118kg that adds water covers rice and soaked, 1 day summer, 2-3 days winter it is desirable to water suction is sufficient,
Water absorption 25%-30% so that the grain of rice complete and crisp for degree, water adopts purified phreatic water or spring water;
(C1) steamed rice:Rice dipping terminates, and drains that rice is standby, and the rice draining be steamed in pot is boiled, and normal pressure steams 25 minutes it is desirable to rice
Outer firmly interior soft, the interior no raw heart, loose do not paste, thoroughly and not rotten, uniformity, steamed rice completes;
(D1) tank that falls ferments:The rice cooking by air-cooled or water-cooled dry in the air cold break up, airing is to 28 DEG C to fall into fermentation
Tank, adds Cordyceps militaris (L.) Link. rice compost 26kg, adds water 230kg, malt yeast 13kg mix homogeneously, plus lactic acid 0.25-0.8kg is adjusted
PH to 4, when temperature drop to 26~30 DEG C, adds yeast wine 5kg, when temperature is raised to 31~32 DEG C, opens rake stirring, makes temperature
It is maintained at 28~30 DEG C, the tank that falls is fermented into swort within 2~3 days;
(E1) after fermentation:After the tank fermentation that falls, fermentation eases up weak, you can the swort of fermentation tank is moved into after fermentation tank,
Control product temperature and room temperature at 15~18 DEG C, static fermentation 20~30 days, so that yeast is fermented further, and improve the local flavor of wine;
(F1) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clear at low temperature
Clear 2~3 days, finally draw the wine liquid supernatant and filter through cotton cake filtering machine;
(G1) decoct wine:Carry out decocting wine, 85 DEG C of temperature, time 15mins to filtering the wine completing with tubular heat exchanger, kill
Go out wherein all microorganisms, in order to preserve, the process of keeping, be also the process of wine and women-sensual pursuits precipitation purification, decoct after drinking, hot wine pours into
In the empty altar of sterilization;
(H1) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis, indocalamus shell after pouring into the empty altar of sterilization, then uses mud head
Or warehouse-in storage after Gypsum Fibrosum sealing, in storage process, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature
5-20 DEG C, annual turning 1-2 time, warehouse-in aging 1 year, obtain wine degree more than 16%, acidity, below 0.45, has rich in Cordyceps militaris (L.) Link.
The high-quality yellow wine of effect composition.
Add Cordyceps militaris (L.) Link. rice compost Cordyceps militaris (L.) Link. rice compost can replace in above-mentioned (D1) falls tank fermentation step
Become and add Cordyceps militaris (L.) Link. rice compost Cordyceps militaris (L.) Link. rice compost, the life to whole Cordyceps militaris (L.) Link. yellow wine in above-mentioned (C1) steamed rice step
Production. art and the final Cordyceps militaris (L.) Link. yellow wine quality obtaining have no significant effect.
Embodiment 2
As shown in Fig. 2 Cordyceps militaris (L.) Link. rice compost is prepared from by following steps:
(a2) prepare fluid medium:Natural in mass ratio nitrogen source dried silkworm chrysalis meal 13, glucose 9, peptone 2, biphosphate
Potassium 1, magnesium sulfate 0.5, ammonium citrate 0.5, VB10.1, the ratio of water 1000, the above material of precise, add water and stir,
It is distributed into 500ml conical flask, absorbent cotton seals, 121-126 DEG C of autoclaving 30mins makes fluid medium;
(b2) prepare rice complex medium:Take clean culture bottle, in culture bottle, first fill 32g rice, add liquid training
Foster base 33ml, polyethylene film seal, 121-126 DEG C of autoclaving 30mins or 100 DEG C sterilizing 8h, be cooled to less than 30 DEG C standby
With rice complex medium is prepared and completed;
(c2) inoculate:In the inoculating hood of sterilizing, inoculated by the requirement of sterile working, by alcohol burner, operation, beats
Open sealed membrane or the bottle cap of culture bottle, draw 5ml Cordyceps militaris strain with asepsis injector, be uniformly injected into and be combined equipped with rice
In the culture bottle of culture medium, cover sealed membrane or bottle cap immediately, inoculation completes;
(d2) send out bacterium:Will be equipped with inoculate Cordyceps militaris strain rice complex medium culture bottle take sterilized
Incubator or culturing room are cultivated, and send out the bacterium stage under dark condition, 18 DEG C of keeping temperature, and humidity 60% is white through 13 days
Color mycelia can cover with rice complex medium, and mycelia is dense luxuriant, send out bacterium and complete;
(e2) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime pair
It carries out photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 little
When, temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the training of Cordyceps militaris (L.) Link. rice
Nutriment.
Now, to contain Active Components in Various Cordyceps militaris Strains the strongest for rice compost, is suitable to produce selenium-enriched cordceps militaris yellow wine.
The technique that this Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine comprises the following steps:
(A2) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white;
(B2) rice dipping:Weigh the rice that 100kg is processed through pure white, first cleaned with water 1 time, drain away the water, be placed in tank, plus
Enter 3kg Cordyceps militaris strain, the 117kg that adds water covers rice and soaked, 1 day summer, 2-3 days winter it is desirable to water suction is sufficient,
Water absorption 25%-30% so that the grain of rice complete and crisp for degree, water adopts purified phreatic water or spring water;
(C2) steamed rice:Rice dipping terminates, and drains rice, adds Cordyceps militaris (L.) Link. rice compost 25kg, then will add Cordyceps militaris (L.) Link. rice
The rice be steamed in pot of compost is boiled, normal pressure steams 26 minutes it is desirable to firmly interior soft, the interior no raw heart outside rice, loose do not paste, thoroughly and not rotten,
Uniformity, steamed rice completes;
(D2) tank that falls ferments:The rice cooking by air-cooled or water-cooled dry in the air cold break up, airing is to 28 DEG C to fall into fermentation
Tank, adds water 235kg, malt yeast 15kg mix homogeneously, plus lactic acid 0.25-0.8kg adjusts PH to 4, when temperature drop to 26~30
DEG C, add yeast wine 5kg, when temperature is raised to 31~32 DEG C, opens rake stirring, keep the temperature at 28~30 DEG C, fall within 2~3 days
Tank is fermented into swort;
(E2) after fermentation:After the tank fermentation that falls, fermentation eases up weak, you can the swort of fermentation tank is moved into after fermentation tank,
Control product temperature and room temperature at 15~18 DEG C, static fermentation 20~30 days, so that yeast is fermented further, and improve the local flavor of wine;
(F2) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clear at low temperature
Clear 2~3 days, finally draw the wine liquid supernatant and filter through cotton cake filtering machine;
(G2) decoct wine:Carry out decocting wine, 90 DEG C of temperature, time 15mins to filtering the wine completing with tubular heat exchanger, kill
Go out wherein all microorganisms, in order to preserve, the process of keeping, be also the process of wine and women-sensual pursuits precipitation purification, decoct after drinking, hot wine pours into
In the empty altar of sterilization;
(H2) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis, indocalamus shell after pouring into the empty altar of sterilization, then uses mud head
Or warehouse-in storage after Gypsum Fibrosum sealing, in storage process, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature
5-20 DEG C, annual turning 1-2 time, warehouse-in aging 1 year, obtain wine degree more than 16%, acidity, below 0.45, has rich in Cordyceps militaris (L.) Link.
The high-quality yellow wine of effect composition.
Add Cordyceps militaris (L.) Link. rice compost can be substituted for fall tank fermentation step at (D2) in above-mentioned (C2) steamed rice step
Middle addition Cordyceps militaris (L.) Link. rice compost, the production technology to whole Cordyceps militaris (L.) Link. yellow wine and the final Cordyceps militaris (L.) Link. yellow wine quality obtaining are no
Significantly affect.
Embodiment 3
As shown in figure 1, Cordyceps militaris (L.) Link. rice compost is prepared from by following steps:
(a3) prepare fluid medium:Natural in mass ratio nitrogen source dried silkworm chrysalis meal 16, glucose 8, peptone 1, biphosphate
Potassium 1.7, magnesium sulfate 0.7, ammonium citrate 0.6, VB10.3, the ratio of water 1000, the above material of precise, add water stirring all
Even, it is distributed into 500ml conical flask, absorbent cotton seals, 121-126 DEG C of autoclaving 30mins makes fluid medium;
(b3) prepare rice complex medium:Take clean culture bottle, in culture bottle, first fill 28g rice, add liquid training
Foster base 36ml, polyethylene film seal, 121-126 DEG C of autoclaving 30mins or 100 DEG C sterilizing 8h, be cooled to less than 30 DEG C standby
With rice complex medium is prepared and completed;
(c3) inoculate:In the inoculating hood of sterilizing, inoculated by the requirement of sterile working, by alcohol burner, operation, beats
Open sealed membrane or the bottle cap of culture bottle, draw 5ml Cordyceps militaris strain with asepsis injector, be uniformly injected into and be combined equipped with rice
In the culture bottle of culture medium, cover sealed membrane or bottle cap immediately, inoculation completes;
(d3) send out bacterium:Will be equipped with inoculate Cordyceps militaris strain rice complex medium culture bottle take sterilized
Incubator or culturing room are cultivated, and send out the bacterium stage under dark condition, 16 DEG C of keeping temperature, humidity 65%, through 14 days, in vain
Color mycelia can cover with culture medium, and mycelia is dense luxuriant, send out bacterium and complete;
(e3) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime pair
It carries out photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 little
When, temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the training of Cordyceps militaris (L.) Link. rice
Nutriment.
Now, to contain Active Components in Various Cordyceps militaris Strains the strongest for rice compost, is suitable to produce selenium-enriched cordceps militaris yellow wine.
The technique that this Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine comprises the following steps:
(A3) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white, meter Xuan Yong content of starch is high,
Fast, the easy gelatinizing of water suction and the soft rice of saccharifying;
(B3) rice dipping:Weigh the rice that 105kg is processed through pure white, first cleaned with water 1 time, drain away the water, be placed in tank, plus
Enter 4kg Cordyceps militaris strain, the 120kg that adds water covers rice and soaked, 1 day summer, 2-3 days winter it is desirable to water suction is sufficient,
Water absorption 25%-30% so that the grain of rice complete and crisp for degree, water adopts purified phreatic water or spring water;
(C3) steamed rice:Rice dipping terminates, and drains that rice is standby, and the rice draining be steamed in pot is boiled, and normal pressure steams 28 minutes it is desirable to rice
Outer firmly interior soft, the interior no raw heart, loose do not paste, thoroughly and not rotten, uniformity, steamed rice completes;
(D3) tank that falls ferments:The rice cooking by air-cooled or water-cooled dry in the air cold break up, airing is to 28 DEG C to fall into fermentation
Tank, adds Cordyceps militaris (L.) Link. rice compost 28kg, adds water 240kg, malt yeast 16kg mix homogeneously, plus lactic acid 0.25-0.8kg is adjusted
PH to 4, when temperature drop to 26~30 DEG C, adds yeast wine 4kg, when temperature is raised to 31~32 DEG C, opens rake stirring, makes temperature
It is maintained at 28~30 DEG C, the tank that falls is fermented into swort within 2~3 days;
(E3) after fermentation:After the tank fermentation that falls, fermentation eases up weak, you can the swort of fermentation tank is moved into after fermentation tank,
Control product temperature and room temperature at 15~18 DEG C, static fermentation 20~30 days, so that yeast is fermented further, and improve the local flavor of wine;
(F3) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clear at low temperature
Clear 2~3 days, finally draw the supernatant and filter through cotton cake filtering machine;
(G3) decoct wine:Carry out decocting wine, 87 DEG C of temperature, time 15mins to filtering the wine completing with tubular heat exchanger, kill
Go out wherein all microorganisms, in order to preserve, the process of keeping, be also the process of wine and women-sensual pursuits precipitation purification, decoct after drinking, hot wine pours into
In the empty altar of sterilization;
(H3) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis, indocalamus shell after pouring into the empty altar of sterilization, then uses mud head
Or warehouse-in storage after Gypsum Fibrosum sealing, in storage process, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature
5-20 DEG C, annual turning 1-2 time, warehouse-in aging 1 year, obtain wine degree more than 16%, acidity, below 0.45, has rich in Cordyceps militaris (L.) Link.
The high-quality yellow wine of effect composition.
Add Cordyceps militaris (L.) Link. rice compost Cordyceps militaris (L.) Link. rice compost can replace in above-mentioned (D3) falls tank fermentation step
Become and add Cordyceps militaris (L.) Link. rice compost Cordyceps militaris (L.) Link. rice compost, the life to whole Cordyceps militaris (L.) Link. yellow wine in above-mentioned (C3) steamed rice step
Production. art and the final Cordyceps militaris (L.) Link. yellow wine quality obtaining have no significant effect.
Embodiment 4
As shown in Fig. 2 Cordyceps militaris (L.) Link. rice compost is prepared from by following steps:
(a4) prepare fluid medium:Natural in mass ratio nitrogen source dried silkworm chrysalis meal 14, glucose 8, peptone 1, biphosphate
Potassium 2, magnesium sulfate 0.7, ammonium citrate 0.9, VB10.2, the ratio of water 1000, the above material of precise, add water and stir,
It is distributed into 500ml conical flask, absorbent cotton seals, 121-126 DEG C of autoclaving 30mins makes fluid medium;
(b4) prepare rice complex medium:Take clean culture bottle, in culture bottle, first fill 30g rice, add liquid training
Foster base 35ml, polyethylene film seal, 121-126 DEG C of autoclaving 30mins or 100 DEG C sterilizing 8h, be cooled to less than 30 DEG C standby
With rice complex medium is prepared and completed;
(c4) inoculate:In the inoculating hood of sterilizing, inoculated by the requirement of sterile working, by alcohol burner, operation, beats
Open sealed membrane or the bottle cap of culture bottle, draw 5ml Cordyceps militaris strain with asepsis injector, be uniformly injected into and be combined equipped with rice
In the culture bottle of culture medium, cover sealed membrane or bottle cap immediately, inoculation completes;
(d4) send out bacterium:Will be equipped with inoculate Cordyceps militaris strain rice complex medium culture bottle take sterilized
Incubator or culturing room are cultivated, and send out the bacterium stage under dark condition, 17 DEG C of keeping temperature, and humidity 65% is white through 15 days
Color mycelia can cover with rice complex medium, and mycelia is dense luxuriant, send out bacterium and complete;
(e4) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime pair
It carries out photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 little
When, temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the training of Cordyceps militaris (L.) Link. rice
Nutriment.
Now, to contain Active Components in Various Cordyceps militaris Strains the strongest for this Cordyceps militaris (L.) Link. rice compost, is suitable to produce selenium-enriched cordceps militaris yellow wine.
The technique that this Cordyceps militaris (L.) Link. rice compost fortification produces Cordyceps militaris (L.) Link. yellow wine comprises the following steps:
(A4) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white;
(B4) rice dipping:Weigh the rice that 100kg is processed through pure white, first cleaned with water 1 time, drain away the water, be placed in tank, plus
Enter 3kg Cordyceps militaris strain, the 117kg that adds water covers rice and soaked, 1 day summer, 2-3 days winter it is desirable to water suction is sufficient,
Water absorption 25%-30% so that the grain of rice complete and crisp for degree;
(C4) steamed rice:Rice dipping terminates, and drains rice, adds Cordyceps militaris (L.) Link. rice compost 27kg, then will add Cordyceps militaris (L.) Link. rice
The rice be steamed in pot of compost is boiled, normal pressure steams 30 minutes it is desirable to firmly interior soft, the interior no raw heart outside rice, loose do not paste, thoroughly and not rotten,
Uniformity, steamed rice completes;
(D4) tank that falls ferments:The rice cooking by air-cooled or water-cooled dry in the air cold break up, airing is to 28 DEG C to fall into fermentation
Tank, adds water 235kg, malt yeast 14kg mix homogeneously, plus lactic acid 0.25-0.8kg adjusts PH to 4, controls 26~30 DEG C of temperature, then
Add yeast wine 5kg, when temperature is raised to 31~32 DEG C, opens rake stirring, keep the temperature at 28~30 DEG C, the tank that falls for 2~3 days ferments
Become swort;
(E4) after fermentation:After the tank fermentation that falls, fermentation eases up weak, you can the swort of fermentation tank is moved into after fermentation tank,
Control product temperature and room temperature at 15~18 DEG C, static fermentation 20~30 days, so that yeast is fermented further, and improve the local flavor of wine;
(F4) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clear at low temperature
Clear 2~3 days, finally draw the wine liquid supernatant and filter through cotton cake filtering machine;
(G4) decoct wine:Carry out decocting wine, 88 DEG C of temperature, time 15mins by filtering the wine completing, kill wherein all micro- lifes
Thing, the process in order to preserve, certainly, is also the process of wine and women-sensual pursuits precipitation purification, decocts after drinking, hot wine pours into the empty altar of sterilization
In;
(H4) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis, indocalamus shell after pouring into the empty altar of sterilization, then uses mud head
Or warehouse-in storage after Gypsum Fibrosum sealing, in storage process, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature
5-20 DEG C, annual turning 1-2 time, warehouse-in aging 1 year, obtain wine degree more than 16%, acidity, below 0.45, has rich in Cordyceps militaris (L.) Link.
The high-quality yellow wine of effect composition.
Add Cordyceps militaris (L.) Link. rice compost can be substituted for fall tank fermentation step at (D4) in above-mentioned (C4) steamed rice step
Middle addition Cordyceps militaris (L.) Link. rice compost, the production technology to whole Cordyceps militaris (L.) Link. yellow wine and the final Cordyceps militaris (L.) Link. yellow wine quality obtaining are no
Significantly affect.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of Cordyceps militaris (L.) Link. rice compost fortification produces the technique of Cordyceps militaris (L.) Link. yellow wine it is characterised in that including following walking
Suddenly:
(A) pure white of rice:By rice polisher, the outer skin of rice is peelled off, reach pure white;
(B) rice dipping:Pure white rice 95-105kg, first with water by meter Chong Xi 1 time, drains away the water, is placed in tank, add rice amount
2%-4% Cordyceps militaris strain, the 117-120kg that adds water covers rice and is soaked, soak time 1-3 days, water absorption 25%-
30% so that the grain of rice complete and crisp for degree, rice dipping terminates;
(C) steamed rice:After rice dipping terminates, drain rice, add Cordyceps militaris (L.) Link. rice compost 25-28kg, then Cordyceps militaris (L.) Link. rice will be added
Pot atmospheric cooking 25-30 minute on the rice of compost, rice reach outer firmly interior soft, the interior no raw heart, loose do not paste, thoroughly and not rotten,
Uniformity, steamed rice completes;
(D) tank that falls ferments:The rice cooking falls in fermentation tank by air-cooled or water-cooled, the 230-240kg that adds water in proportion, malt yeast
13-16kg mix homogeneously, plus lactic acid 0.25-0.8kg regulation PH to 4, when temperature drop to 26~30 DEG C, add yeast wine 4-
6kg, when temperature is raised to 31~32 DEG C, opens rake stirring, keeps the temperature at 28~30 DEG C, the tank that falls is fermented into swort within 2~3 days;
(E) after fermentation:The swort of fermentation tank is moved into after fermentation tank, controls product temperature and room temperature at 15~18 DEG C, static fermentation 20
~30 days;
(F) clarification squeezing:First with plate and frame type filter-press, wine liquid and distiller grains are separated, then wine liquid is clarified at low temperature 2~3
My god, finally draw the wine liquid supernatant and filter through cotton cake filtering machine;
(G) decoct wine:Carry out decocting wine by filtering the wine completing, temperature is 85~90 DEG C, time 15mins, decoct after drinking, hot wine is filled
Enter in the empty altar of sterilization;
(H) preserve aging:Hot wine is bandaged with sterilized Folium Nelumbinis or indocalamus shell after pouring into the empty altar of sterilization, then with mud head or stone
Warehouse-in storage after cream sealing, warehouse-in aging 1 year, obtain wine degree more than 16%, Cordyceps militaris (L.) Link. yellow wine below 0.45 for the acidity;
(C) the Cordyceps militaris (L.) Link. rice compost described in steamed rice step is prepared from by following steps:
A () prepares fluid medium:Weigh natural nitrogen source dried silkworm chrysalis meal 13-16, glucose 8-10, peptone 1-3, phosphorus in mass ratio
Acid dihydride potassium 1-2, magnesium sulfate 0.5-1, ammonium citrate 0.5-1, VB10.1-0.3, water 1000, stir, are distributed into 500ml
Conical flask, absorbent cotton seal, 121-126 DEG C of autoclaving 30mins, be cooled to less than 30 DEG C standby, fluid medium has been prepared
Become;
B () prepares rice complex medium:Take clean culture bottle, in culture bottle, first fill 28-32g rice, add liquid culture
Base 33-36ml, polyethylene film seals, 121-126 DEG C of autoclaving 30mins or 100 DEG C of sterilizing 8h, is cooled to less than 30 DEG C
Standby, rice complex medium is prepared and is completed;
C () is inoculated:In the inoculating hood of sterilizing, operation by alcohol burner, draw 5ml Cordyceps militaris bacterium with asepsis injector
Kind, it is uniformly injected in the culture bottle equipped with rice complex medium, covers sealed membrane or bottle cap immediately, rice complex medium connects
Plant and complete;
D () sends out bacterium:The culture bottle that will be equipped with inoculating the rice complex medium of Cordyceps militaris strain takes sterilized culture
Case or culturing room are cultivated, under dark condition, keeping temperature 16-18 DEG C, and humidity 60-65%, 13-15 days, in culture bottle
White hypha is dense luxuriant, covers with rice complex medium, sends out bacterium and completes;
(e) annesl flower bud:After the completion of rice complex medium sends out bacterium, in the incubator of sterilizing or culturing room, daytime enters to it
Row photo-irradiation treatment, intensity of illumination 100~200 lux, 12 hours time, temperature 20-21 DEG C;Night keeps dark 12 hours,
Temperature 14-16 DEG C, after 7~10 days, mycelium becomes orange-yellow or yellow, and annesl flower bud completes, and obtains the culture of Cordyceps militaris (L.) Link. rice
Material.
2. a kind of Cordyceps militaris (L.) Link. rice compost fortification produces the technique of Cordyceps militaris (L.) Link. yellow wine as claimed in claim 1, and it is special
Levy and be:Described Cordyceps militaris (L.) Link. rice compost is substituted for by addition in (C) steamed rice step in falling tank fermentation step at (D) and adds
Enter.
3. described a kind of Cordyceps militaris (L.) Link. rice compost fortification as arbitrary in claim 1 to 2 produces the work of Cordyceps militaris (L.) Link. yellow wine
Skill it is characterised in that:Described meter Xuan Yong content of starch is high, the soft rice of absorb water fast, easy gelatinizing and saccharifying.
4. a kind of Cordyceps militaris (L.) Link. rice compost fortification produces the technique of Cordyceps militaris (L.) Link. yellow wine as claimed in claim 3, and it is special
Levy and be:Water adopts purified phreatic water or spring water.
5. a kind of Cordyceps militaris (L.) Link. rice compost fortification produces the technique of Cordyceps militaris (L.) Link. yellow wine as claimed in claim 3, and it is special
Levy and be:(G) decoct in wine step and wine is decocted using tubular heat exchanger.
6. a kind of Cordyceps militaris (L.) Link. rice compost fortification produces the technique of Cordyceps militaris (L.) Link. yellow wine as claimed in claim 3, and it is special
Levy and be:(H) in storage aging step, storeroom answers well-ventilated, prevents from drenching with rain, and the warehouse of storage wine keeps room temperature 5-20 DEG C,
Turning every year 1-2 time.
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Cited By (4)
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CN107267348A (en) * | 2017-08-24 | 2017-10-20 | 宜智萱(天津)生物科技有限公司 | A kind of new aweto yellow wine preparation method |
CN108546619A (en) * | 2018-07-25 | 2018-09-18 | 宁德市大源丰农业发展有限公司 | A kind of quinoa time of childbirth wine and preparation method thereof |
CN110373299A (en) * | 2019-08-20 | 2019-10-25 | 山西省农业科学院食用菌研究所 | The production method of silkworm chrysalis Cordyceps sinensis stilbene ginseng series of products |
CN114907935A (en) * | 2022-07-05 | 2022-08-16 | 嘉善子陵滩酒业有限公司 | Production process of yellow rice wine |
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Cited By (4)
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