CN106344429B

CN106344429B - A kind of needle mushroom Essence and preparation method thereof

Info

Publication number: CN106344429B
Application number: CN201610866005.1A
Authority: CN
Inventors: 董娟娥; 梁宗锁; 刘景煜; 李晨; 肖林刚
Original assignee: Northwest A&F University
Current assignee: Northwest A&F University
Priority date: 2016-09-29
Filing date: 2016-09-29
Publication date: 2019-06-18
Anticipated expiration: 2036-09-29
Also published as: CN106344429A

Abstract

The present invention relates to a kind of needle mushroom Essence and preparation method thereof, formula contains flammulina velutipes, glycerol, 1,2-PD, Sodium Hyaluronate, trehalose, citric acid, D-pantothenyl aleohol, niacinamide, stabilizer, preservative and water.Needle mushroom Essence is using natural flammulina velutipes as core component obtained by the present invention, with the maintaining beauty and keeping youngs such as moisturizing, anti-oxidant, anti-aging, firm skin, contained Sodium Hyaluronate is natural high moisturizing factor, it is aided with the ingredients such as trehalose, niacinamide, D-pantothenyl aleohol again, needle mushroom Essence is set to have the function of high moisturizing, whitening, firm skin, skin can also be made to keep cell activity in the adverse circumstances such as high temperature, high and cold, intensive ultraviolet radiation, improve anti-adversity ability.

Description

A kind of needle mushroom Essence and preparation method thereof

Technical field

The invention belongs to cosmetic technical fields, and in particular to a kind of needle mushroom Essence and preparation method thereof.

Background technique

Needle mushroom [Flammulinavelutipes (CurtFr) Sing.] is load Zoopagales money Pseudomonas, is a kind of medicine Eat dual-purpose bacterium.Main component has polysaccharide, albumen, flavones, polyphenol and amino acid etc..Wherein, polysaccharide is as main effective component One of, there are the pharmacological actions such as antitumor, anti-oxidant, raising immunity.

From the point of view of medical angle, healthy skin should have the feature that smooth tender, fine and transparent, moisten it is flawless, High resilience.Many factors in living environment, such as the increases of social pressures, irregular, living environment the pollution of diet It will lead to skin histology underactivity, obstacle occur in cell metabolism, microcirculation, and then show pachylosis, drying, dim It is light, lack phenomena such as gloss, wrinkle, pigment deposition.

Common toner has toner, smoothing toner, astringent lotion etc., and the trophism of Essence is higher than common toner, It can releive tight, dry skin.Essence is typically free of alcohol, non-stimulated to skin, more preferably absorption nutrition.Essence Effective component is not only merely resting on skin surface, can also reach the deep layer of skin, directly participate in the metabolism of cell, Skin can be allowed to reply water profit state in moment.

Summary of the invention

It is for the defects in the prior art and insufficient, it can permanent moisturizing, holding flesh the purpose of the present invention is developing one kind Skin cell activity and the Essence for having whitening, careful skin effect propose a kind of needle mushroom Essence and preparation method thereof, The Essence not stimulating components such as spirituosity, it is raw materials used safe and non-toxic, it is good with skin affinity, and preparation method is simple, It is green safe, it is suitble to mass production.

In order to achieve the above objectives, the technical scheme adopted by the invention is as follows:

A kind of needle mushroom Essence, including flammulina velutipes, glycerol, 1,2-PD, Sodium Hyaluronate, trehalose, lemon Lemon acid, D-pantothenyl aleohol, niacinamide, stabilizer, preservative and water.

Specifically, according to the mass fraction, including 0.05~0.25 part of flammulina velutipes, 1~10 part of glycerol, 1,2- the third two 0.5~1.5 part of alcohol, 0.1~0.5 part of Sodium Hyaluronate, 0.5~2 part of trehalose, 0.05~0.2 part of citric acid, D-pantothenyl aleohol 0.5 ~2 parts, 0.5~2 part of niacinamide, 0.2~0.5 part of stabilizer, 0.05 part of preservative, surplus is water, and the total amount of mentioned component is 100 parts.

More specifically, the flammulina velutipes are thick slightly to raise flammulina velutipes to needle mushroom progress using water extraction Extract, then flammulina velutipes crude extract is carried out by aqueous two-phase system flammulina velutipes are obtained by extraction；The aqueous two-phase Double-aqueous phase system used in extraction includes ethyl alcohol and inorganic salts.

In addition, the inorganic salts include one of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ammonium sulfate and sodium carbonate.

In addition, the mass concentration of ethyl alcohol is 24%~30% in the double-aqueous phase system, the mass concentration of inorganic salts is 21%~24%.

Further, needle mushroom is carried out slightly raising flammulina velutipes crude extract including: by weight using water extraction Meter, takes 100 parts of golden needle fungus, is added 1000 parts of deionized water, boiling water bath refluxing extraction, extraction time 60min, extraction time 2 Secondary, combined extract, concentration constant volume obtains flammulina velutipes crude extract；

Flammulina velutipes crude extract is carried out that flammulina velutipes are obtained by extraction to include: by water extraction by aqueous two-phase system It obtains flammulina velutipes crude extract to be extracted by the double-aqueous phase system that ethyl alcohol is formed with inorganic salts, take under double-aqueous phase system Layer solution obtain flammulina velutipes extraction phase, by flammulina velutipes extraction phase by molecular weight be 8000~14000 bag filters into Row retention, trapped substance carry out reduced vacuum and are concentrated up to flammulina velutipes.

Alternatively, the golden needle fungus is fresh needle mushroom drying gained, drying temperature is 50 DEG C, drying time 48h, powder Broken resulting golden needle fungus crosses 20 meshes.

Alternatively, the preservative includes one of sodium sorbate and methyl p-hydroxybenzoate, stabilizer includes spitting One of temperature 80, Macrogol 4000 and Acritamer 940.

The preparation method of the needle mushroom Essence takes 0.05~0.5 part of flammulina velutipes, molten with 10 parts of deionized water Solution is A phase；0.5~2 part of trehalose is weighed, 0.05~0.2 part of citric acid, 0.5~2 part of niacinamide, 0.05 part of preservative, is used 10 parts of deionized water dissolvings are B phase；Merging A, B phase, 0.1~0.5 part of Sodium Hyaluronate of addition, 0.2~0.5 part of stabilizer, It naturally is C phase after dissolving；1~10 part of glycerol is weighed, 0.5~1.5 part of 1,2-PD, 0.5~2 part of D-pantothenyl aleohol, C phase is added In, deionized water is added to 100 parts, is stirred evenly to get needle mushroom Essence.

Advantages of the present invention are as follows:

1, the present invention chooses ethyl alcohol and inorganic salts and cooperatively forms extraction system, to the extraction yields of flammulina velutipes up to 85% ~91%, to the recovery rates of flammulina velutipes up to 74.3%~82.9%, gained flammulina velutipes purity up to 63.1%~ 67.3%, this method is high and easy to operate to the separative efficiency of flammulina velutipes, with conventional polymer or ionic liquid and salt The system of formation is compared, and extraction cost is greatly reduced, and overcomes the recycling of target component difficulty and ionic liquid toxicity in polymer Larger disadvantage.Whole process simple process, separative efficiency is high, low in cost, safe and non-toxic, can be used for large-scale industry metaplasia It produces；

2, flammulina velutipes have moisturizing and trophism to skin, have anti-oxidant, antitumor, anti-aging, raising immune The effect of power.Facial treatment essence liquid is developed by raw material of flammulina velutipes, it is natural environmental-protective, safe and non-toxic, it is nonirritant；

3, the flammulina velutipes that needle mushroom Essence contains in the present invention can improve skin elasticity with delay skin aging, There is certain anti-radiation, moisture-keeping function；Glycerol, 1,2- propylene glycol, Sodium Hyaluronate etc. have long-acting high moisture-keeping function；D- is general Alcohol is penetrating deep into property moisturizer, and moistening effect goes directly skin inner layer, fundamentally plays moistening effect；Trehalose can make skin Cell activity is kept in the adverse circumstances such as high temperature, arid, intensive ultraviolet radiation, and one layer of protection is formed outside cuticula Film；Niacinamide is vitamin B₃The generally acknowledged skin anti-aging composition of a kind of derivative and cosmetic dermatology field, lemon The effects of acid can accelerate cutin metabolism, facilitate falling off for the middle melanin of skin, and the two plays anti-aging, whitening jointly.

Detailed description of the invention

Fig. 1 is the Hydroxyl radical-scavenging for the flammulina velutipes that aqueous two-phase system and water extraction extract in embodiment 1 Rate comparison diagram；

Fig. 2 is needle mushroom Essence skin moisturization figure in embodiment 2；

It elaborates below in conjunction with the specific embodiment that inventor provides to technical solution of the present invention.

Specific embodiment

A kind of preparation method of flammulina velutipes Essence of the invention, comprising the following steps:

Step 1: by weight, taking 100 parts of golden needle fungus, is added 1000 parts of deionized water, boiling water bath refluxing extraction, Extraction time 60min, extraction time 2 times, combined extract is concentrated, and constant volume obtains flammulina velutipes crude extract；

Step 2: Thick many candies extract is handled with aqueous two-phase system, polysaccharide enrichment obtains flammulina velutipes extraction phase；It is double The double-aqueous phase system of water phase extraction, solvent are water, and solute is ethyl alcohol and inorganic salts.

Step 3: dialysis treatment is carried out to flammulina velutipes extract liquor obtained by step 2, to remove inorganic salts, by dialyzate 55 DEG C of reduced vacuum concentrations, concentrate are freeze-dried to obtain flammulina velutipes.

Step 4: taking 0.05~0.25 part of flammulina velutipes in step 3, and 1~10 part of glycerol, 1,2-PD 0.5~ 1.5 parts, 0.1~0.5 part of Sodium Hyaluronate, 0.5~2 part of trehalose, 0.05~0.2 part of citric acid, 0.5~2 part of D-pantothenyl aleohol, 0.5~2 part of niacinamide, 0.01 part of preservative, deionized water is added to 100 parts, is stirred evenly, i.e., by 0.2~0.5 part of stabilizer Obtain needle mushroom Essence.

In step 1, golden needle fungus is fresh needle mushroom drying gained, and drying temperature is 50 DEG C, drying time 48h, is crushed Resulting golden needle fungus crosses 20 meshes, sealed storage.

In step 2, the inorganic salts of double-aqueous phase system are dipotassium hydrogen phosphate, in potassium dihydrogen phosphate, ammonium sulfate, sodium carbonate One kind, ethyl alcohol mass concentration is 24~30% in double-aqueous phase system, and Inorganic salt is 21~24%.

In step 3, dialysis bag retention molecular weight used in dialysis treatment is 8000~14000.

In step 4, stabilizer is one of Tween-80, polyethylene glycol-4000 and Acritamer 940, and preservative is mountain One of pears acid sodium and methyl p-hydroxybenzoate.

The preparation method of Essence in step 4 are as follows:

(1) 0.05~0.25 part of flammulina velutipes of purifying is taken, is dissolved with 10 parts of deionized water, is A phase；

(2) 0.5~2 part of trehalose is weighed, 0.05~0.2 part of citric acid, 0.5~2 part of niacinamide, 0.05 part of preservative, It is B phase with 10 parts of deionized water dissolvings；

(3) merge A, B phase, be added 0.1~0.5 part of Sodium Hyaluronate, 0.2~0.5 part of stabilizer, stir evenly, stand For 24 hours, make sufficiently to dissolve naturally, be C phase；

(4) 1~10 part of glycerol is weighed, 0.5~1.5 part of 1,2-PD, 0.5~2 part of D-pantothenyl aleohol, is added in C phase, Deionized water is added to 100 parts, is stirred evenly to get needle mushroom Essence.

The present invention provides the formula and preparation method of a kind of needle mushroom Essence, and the safe and non-stimulating property of the formula material is protected Wet skin, anti-aging, whitening effect are good, and preparation method is simple and easy to operate.

Flammulina velutipes used are obtained by acupuncture needle mushroom extracting solution recycle after aqueous two-phase extraction in the present invention.Double-aqueous phase system (Aqueous two-phase systems, ATPS) refers to that the aqueous solution of two kinds of hydrophilic compounds mixes under certain mass concentration The two not miscible aqueous phase systems spontaneously formed after conjunction.Aqueous two-phase extraction is a kind of novel liquid-liquid extraction and separation technology, behaviour Work is simple, separative efficiency is high, separation condition is mild and technique is easy amplification, has the prospect of industrial separation natural products.It passes The extraction system of system is often cooperatively formed with high polymer and salt, and the cost is relatively high for high polymer, is difficult to recycling and reusing.Present invention choosing It takes ethyl alcohol and inorganic salts to cooperatively form extraction system, reduces extraction cost and toxicity, base can be established for next step industrialized production Plinth.

Formula of the invention is by flammulina velutipes, glycerol, 1,2-PD, Sodium Hyaluronate, trehalose, citric acid, D- Panthenol, niacinamide, stabilizer, preservative composition.Studies have shown that flammulina velutipes can improve skin bullet with delay skin aging Property, there is certain anti-radiation, moisture-keeping function, while there is anti-oxidant, antitumor, raising immunity and antibacterial effect, this hair Bright to test needle mushroom Essence skin moisturization, moisturizing rate such as Fig. 1 shows that needle mushroom Essence moistening effect is significant Glycerine-Aqueous Solution higher than 6%, moisture preserving time are also lasting compared with 6% Glycerine-Aqueous Solution.

Glycerol be traditional excellent moisturizer, it is quality-high and inexpensive, but using when have sticky sense, high concentration has certain thorn to skin Swash property；1,2-PD, which has, both shares moistening effect with glycerol but compared with glycerol milder with moisturizing intensity as glycerols Increase, the irritation of glycerol can be alleviated, eliminates the sticky sense that glycerol uses；Sodium Hyaluronate is generally acknowledged efficient moisture-retention agent, Excellent moisture-keeping function is still played in dry environment (air humidity is 30% or so), to human body without any injury；D-pantothenyl aleohol For the moisturizer penetrated deep into, the growth of epithelial cell is stimulated, promotes wound healing, plays antiinflammation；Above-mentioned four kinds of moisturizer Auxiliary flammulina velutipes are shared to play more comprehensively deeper into more comfortable moistening effect.

Trehalose is referred to as " super sun-proof moisturizing factor ", and the application on cosmetics is based on it with excellent guarantor Hold cell viability and the active characteristic of large biological molecule, Skin Cell (especially epidermal cell) is in high temperature, drying, strong ultraviolet Under the environment such as beta radiation, easily drying out occurs keratinization or even death falls off makes skin damage.Trehalose is in such case One layer of special protective film can be formed in cell surface down, not only moisten Skin Cell, external heat can also be radiated It goes out, to protect skin that will not be damaged, enables skin health natural, flexible.

Citric acid also known as citric acid belong to natural organic tartaric acid, are mainly used for accelerating cutin update, the update of cutin helps In the peeling of the middle melanin of skin, the receipts of pore are thin etc.；Niacinamide is vitamin B₃A kind of derivative and beauty treatment skin The generally acknowledged skin anti-aging composition of scientific domain；Citric acid and niacinamide collective effect, having needle mushroom Essence improves skin Matter, whitening and anti-aging effect.

In addition, being added to safe and non-toxic stabilizer, and peace to increase the stability of Essence, guaranteeing certain consistency The preservative of full high-efficiency broad spectrum.Each component complements each other, is indispensable, plays moisturizing, whitening, sun-proof, anti-aging, improvement jointly The effect of skin quality.Stabilizer is one of Tween-80, polyethylene glycol-4000 and Acritamer 940, through testing, three's heat resistance Cold resistance indifference, compared with Macrogol 4000, Acritamer 940 dosage is smaller, and Tween 80 is more suitable for the class containing essential oil The stabilizer of Essence.

In order to better understand the present invention, it is further illustrated below with reference to specific embodiment.

Embodiment 1:

Fresh needle mushroom is placed in clean stainless steel pallet, drying to constant weight in air dry oven, and temperature is 50 DEG C, when Between be 48h, Universalpulverizer crush, cross 20 meshes, obtain golden needle fungus；

The extraction process of water extraction includes: to take 100 parts of golden needle fungus to set in a round bottom flask, and deionized water 1000 is added Part, stirring is mixed thoroughly needle mushroom powder with deionized water, and boiling water bath refluxing extraction 2 times, flow back 60min every time, mixes It closes liquid and is centrifuged 20min with supercentrifuge 7000r/min, make needle mushroom granulated slag precipitating completely, take supernatant, 55 DEG C of vacuum subtract Pressure is concentrated into 100 parts, adds 500 parts of dehydrated alcohols, is sufficiently stirred, and 7000r/min is centrifuged 10min, removes supernatant, precipitating It is freeze-dried to obtain needle mushroom Thick many candies.

The extraction process of aqueous two-phase system includes: to obtain needle mushroom Thick many candies by above-mentioned water extraction, takes 10 portions of needle mushrooms Thick many candies, with 100 parts of deionized water dissolvings, dehydrated alcohol, which is added, makes its mass concentration 28%, and adding ammonium sulfate makes it Mass concentration is 23%, is sufficiently vibrated, and static 10min, mixed liquor supercentrifuge 7000r/min centrifugation 10min make to be layered Completely, lower layer's solution is taken to obtain flammulina velutipes extraction phase, it is 8000~14000 that extract liquor, which is transferred to molecular cut off, In bag filter, with deionized water dialysis 6 times, every time 6 hours.55 DEG C of reduced vacuum concentrations of dialyzate, concentrate freeze-drying To flammulina velutipes.

The purity of needle mushroom Thick many candies obtained by water extraction is 23.5%, and flammulina velutipes purity obtained by aqueous two-phase system is 67.3%.It is detected to gained flammulina velutipes antioxidation is extracted by index of Scavenging action to hydroxyl free radical, as a result such as Fig. 1 It is shown, show that the Scavenging action to hydroxyl free radical of flammulina velutipes obtained by aqueous two-phase system is significantly higher than the resulting gold of water extraction Needle mushroom polysaccharide.

Embodiment 2:

Needle mushroom drying means and needle mushroom Thick many candies preparation process are the same as embodiment 1.

Needle mushroom Essence prescription are as follows: 0.1 part of flammulina velutipes, 1 part of glycerol, 1 part of 1,2-PD, Sodium Hyaluronate 0.3 part, 1 part of trehalose, 0.1 part of citric acid, 0.5 part of D-pantothenyl aleohol, 1 part of niacinamide, Acritamer 940 is 0.2 part, sodium sorbate 0.05 part, 94.75 parts of deionized water.

Needle mushroom Essence preparing process are as follows:

0.1 part of flammulina velutipes is taken, is A phase with 10 parts of deionized water dissolvings；

Weigh 1 part of trehalose, it is 0.1 part of citric acid, 1 part of niacinamide, 0.05 part of sodium sorbate, water-soluble with 10 parts of deionizations Solution is B phase liquid；

Merge A, B phase, be added 0.3 part of Sodium Hyaluronate, Acritamer 940 is 0.2 part, is sufficiently stirred, and stands for 24 hours, makes to fill Divide nature dissolution, is C phase；

1 part of glycerol is weighed, 1 part of 1,2-PD, 0.5 part of D-pantothenyl aleohol, is added in C phase, adds deionized water to 100 Part, it stirs evenly to get needle mushroom Essence.

The extraction yield of the flammulina velutipes of the present embodiment is 91%, recovery rate 81.4%, purity 67.3%.

The moistening effect of the needle mushroom Essence of preparation is tested, the Glycerine-Aqueous Solution with deionized water and 6% is Control, measures and calculates the moisturizing rate and moisture preserving time of Essence, moisturizing rate calculation formula are as follows: moisturizing rate/%=(uses essence Skin moisture content-uses skin moisture content before Essence after liquid)/using skin moisture content × 100 before Essence, such as scheme Shown in 2, show that needle mushroom Essence has good moisture retention to skin, the glycerol-that performance of keeping humidity is significantly higher than 6% is water-soluble Liquid, and in 100min moisturizing rate still up to 50% or more.

Embodiment 3:

Needle mushroom drying means and needle mushroom Thick many candies preparation process are the same as embodiment 1.Unlike: second in double-aqueous phase system Alcohol mass concentration is 30%, Inorganic salt 24%.

Needle mushroom Essence prescription are as follows: 0.25 part of flammulina velutipes, 10 parts of glycerol, 0.5 part of 1,2-PD, hyaluronic acid 0.1 part of sodium, 2 parts of trehalose, 0.05 part of citric acid, 0.5 part of D-pantothenyl aleohol, 2 parts of niacinamide, Acritamer 940 is 0.5 part, sorbic acid 0.05 part of sodium, 86.05 parts of deionized water.

Needle mushroom Essence preparing process is the same as embodiment 2.

The extraction yield of the flammulina velutipes of the present embodiment is 89%, recovery rate 75.7%, purity 63.1%.Aqueous two-phase When ethyl alcohol and ammonium sulfate mass concentration increase to 30% and 24% respectively in system, the extraction yield of gained flammulina velutipes is extracted Rate and purity have a degree of reduction, illustrate that matching between ethyl alcohol and inorganic salts compares flammulina velutipes in double-aqueous phase system Effect of extracting have a certain impact.

Embodiment 4:

Needle mushroom drying means and needle mushroom Thick many candies preparation process are the same as embodiment 1.Unlike: second in double-aqueous phase system The mass concentration of alcohol is 26%, Inorganic salt 22%.

Needle mushroom Essence prescription are as follows: 0.1 part of flammulina velutipes, 5 parts of glycerol, 1.5 parts of 1,2-PD, Sodium Hyaluronate 0.3 part, 1 part of trehalose, 0.05 part of citric acid, 1 part of D-pantothenyl aleohol, 2 parts of niacinamide, Acritamer 940 is 0.2 part, sodium sorbate 0.05 part, 88.8 parts of deionized water.

Needle mushroom Essence preparing process is with embodiment 2.

The extraction yield of the flammulina velutipes of the present embodiment is 88%, recovery rate 82.9%, purity 65.4%.

Embodiment 5:

Needle mushroom drying means and needle mushroom Thick many candies preparation process are the same as embodiment 1.Unlike: second in double-aqueous phase system The mass concentration of alcohol is 24%, Inorganic salt 21%.

Needle mushroom Essence prescription are as follows: 0.05 part of flammulina velutipes, 1 part of glycerol, 1 part of 1,2-PD, Sodium Hyaluronate 0.5 part, 1 part of trehalose, 0.2 part of citric acid, 2 parts of D-pantothenyl aleohol, 1 part of niacinamide, Acritamer 940 is 0.3 part, sodium sorbate 0.05 part, 92.9 parts of deionized water.

Needle mushroom Essence preparing process is the same as embodiment 2.

The extraction yield of the flammulina velutipes of the present embodiment is 85%, recovery rate 74.3%, purity 64.6%.

Cutaneous sense, stability, security test are carried out to embodiment 2~5.Cutaneous sense tester is 10 skins 20~30 years old personage in order；The appearance of needle mushroom Essence, smell, cold-resistant and resistance to is detected according to QBT 2660-2004 Thermal stability.

Needle mushroom Essence sensory evaluation criteria is as shown in table 1:

1 needle mushroom Essence sensory evaluation criteria of table

Sensory evaluation total score=appearance × 0.1+ smell × 0.1+ skin sense × 0.3+ moisture retention × wrinkle resistance × 0.2 0.3+

Needle mushroom Essence Analyses Methods for Sensory Evaluation Results is as shown in table 2, shows that the Essence sense organ of embodiment 2 and embodiment 4 is commented Valence total score is higher, and glycerol, 1,2-PD, Sodium Hyaluronate, D-pantothenyl aleohol dosage are less in embodiment 2, and moisture retention is best, makes It is best with rear skin sense, it may have good wrinkle resistance.The advantage of embodiment 4 is to be added to more niacinamide, is had better Anti-wrinkle effect.

2 needle mushroom Essence Analyses Methods for Sensory Evaluation Results of table

Embodiment	Appearance	Smell	Skin sense	Moisture retention	Wrinkle resistance	Total score
							2	9	8	9	9	7	8.5
3	7	8	7	8	6	7.2
							4	8	9	7	9	8	8.1
5	9	6	6	8	7	7.1

The results are shown in Table 3 for needle mushroom Essence stability and safety examination: the needle mushroom essence in embodiment 2~5 Liquid is heat-resisting, cold resistance is good, and highly-safe, without allergic reaction after use.

3 needle mushroom Essence stability of table and safety examination result

Embodiment 6:

The present embodiment as different from Example 2 inorganic salts using dipotassium hydrogen phosphate.

The extraction yield of the flammulina velutipes of the present embodiment is 81.5%, recovery rate 65.7%, purity 62.7%.

Embodiment 7:

The present embodiment as different from Example 2 inorganic salts using sodium carbonate.

The extraction yield of the flammulina velutipes of the present embodiment is 76.8%, recovery rate 63.2%, purity 59.1%.

Embodiment 8:

The present embodiment is as different from Example 2:

Needle mushroom Essence prescription and configuration technique with embodiment 2, unlike: be free of Sodium Hyaluronate.

The moistening effect of needle mushroom Essence in the embodiment is tested, as a result such as table 4, the display guarantor of embodiment 2 Wet rate is apparently higher than embodiment 8, and four kinds of moisturizer share, and moistening effect can significantly enhance.

8 moistening effect contrast table of 4 embodiment 2 of table and embodiment

。

Claims

1. a kind of needle mushroom Essence, which is characterized in that including flammulina velutipes, glycerol, 1,2-PD, Sodium Hyaluronate, Trehalose, citric acid, D-pantothenyl aleohol, niacinamide, stabilizer, preservative and water；

According to the mass fraction, including 0.05~0.25 part of flammulina velutipes, 1~10 part of glycerol, 0.5~1.5 part of 1,2-PD, 0.1~0.5 part of Sodium Hyaluronate, 0.5~2 part of trehalose, 0.05~0.2 part of citric acid, 0.5~2 part of D-pantothenyl aleohol, niacinamide 0.5~2 part, 0.2~0.5 part of stabilizer, 0.05 part of preservative, surplus is water, and the total amount of mentioned component is 100 parts；

The flammulina velutipes are to carry out slightly raising flammulina velutipes crude extract to needle mushroom using water extraction, then pass through double Water phase extraction extracts flammulina velutipes crude extract, retention of dialysing obtains flammulina velutipes；

Double-aqueous phase system used in the aqueous two-phase system includes ethyl alcohol and inorganic salts；

Needle mushroom is carried out slightly raising flammulina velutipes crude extract including: by weight, to take golden needle fungus using water extraction 100 parts, 1000 parts of deionized water of addition, boiling water bath refluxing extraction, extraction time 60min, extraction time 2 times, combined extract, Concentration constant volume obtains flammulina velutipes crude extract；

Flammulina velutipes crude extract is extracted by aqueous two-phase system, retention of dialysing obtain flammulina velutipes include: by Water extraction obtains flammulina velutipes crude extract and is extracted by the double-aqueous phase system that ethyl alcohol is formed with inorganic salts, takes aqueous two-phase body Lower layer's solution of system obtains flammulina velutipes extraction phase, and flammulina velutipes extraction phase is saturating for 8000~14000 by molecular weight Analysis bag is retained, and trapped substance carries out reduced vacuum and is concentrated up to flammulina velutipes.

2. needle mushroom Essence as described in claim 1, which is characterized in that the inorganic salts include dipotassium hydrogen phosphate, phosphorus One of acid dihydride potassium, ammonium sulfate and sodium carbonate.

3. needle mushroom Essence as described in claim 1, which is characterized in that the quality of ethyl alcohol is dense in the double-aqueous phase system Degree is 24%~30%, and the mass concentration of inorganic salts is 21%~24%.

4. needle mushroom Essence as described in claim 1, which is characterized in that the golden needle fungus dries institute for fresh needle mushroom , drying temperature is 50 DEG C, drying time 48h, crushes resulting golden needle fungus and crosses 20 meshes.

5. needle mushroom Essence as described in claim 1, which is characterized in that the preservative includes sodium sorbate and to hydroxyl One of yl benzoic acid methyl esters, stabilizer include one of Tween 80, Macrogol 4000 and Acritamer 940.

6. the preparation method of needle mushroom Essence described in claim 1-5 any claim, which is characterized in that take needle mushroom It 0.05~0.5 part of polysaccharide, is dissolved with 10 parts of deionized water, is A phase；0.5~2 part of trehalose is weighed, citric acid 0.05~0.2 Part, 0.5~2 part of niacinamide, 0.05 part of preservative, be B phase with 10 parts of deionized water dissolvings；Merge A, B phase, hyalomitome is added 0.1~0.5 part of sour sodium, 0.2~0.5 part of stabilizer, be C phase after dissolving naturally；1~10 part of glycerol is weighed, 1,2-PD 0.5 It~1.5 parts, 0.5~2 part of D-pantothenyl aleohol, is added in C phase, adds deionized water to 100 parts, stir evenly to get needle mushroom essence Liquid.