CN106305138B - Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests - Google Patents
Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests Download PDFInfo
- Publication number
- CN106305138B CN106305138B CN201610707414.7A CN201610707414A CN106305138B CN 106305138 B CN106305138 B CN 106305138B CN 201610707414 A CN201610707414 A CN 201610707414A CN 106305138 B CN106305138 B CN 106305138B
- Authority
- CN
- China
- Prior art keywords
- purple
- seeds
- culturing
- soil
- mushroom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 69
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 29
- 238000012364 cultivation method Methods 0.000 title claims abstract description 15
- 241000233866 Fungi Species 0.000 claims abstract description 28
- 241000939957 Lactarius vividus Species 0.000 claims abstract description 4
- 238000012258 culturing Methods 0.000 claims description 36
- 239000002689 soil Substances 0.000 claims description 33
- 241000196324 Embryophyta Species 0.000 claims description 25
- 239000000758 substrate Substances 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 18
- 239000010451 perlite Substances 0.000 claims description 18
- 235000019362 perlite Nutrition 0.000 claims description 18
- 239000010455 vermiculite Substances 0.000 claims description 18
- 235000019354 vermiculite Nutrition 0.000 claims description 18
- 229910052902 vermiculite Inorganic materials 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 241000018650 Pinus massoniana Species 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 11
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000035764 nutrition Effects 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- 239000008223 sterile water Substances 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 6
- 229960002413 ferric citrate Drugs 0.000 claims description 6
- 238000011010 flushing procedure Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 208000015181 infectious disease Diseases 0.000 claims description 6
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 238000009331 sowing Methods 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 6
- 235000020679 tap water Nutrition 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000010454 slate Substances 0.000 claims description 4
- 230000003592 biomimetic effect Effects 0.000 claims 1
- 238000012136 culture method Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 4
- 241000221986 Russulaceae Species 0.000 abstract 2
- 241000221987 Russula Species 0.000 abstract 1
- 238000009313 farming Methods 0.000 abstract 1
- 235000011609 Pinus massoniana Nutrition 0.000 description 11
- 239000011159 matrix material Substances 0.000 description 9
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 8
- 235000013430 Pinus taiwanensis Nutrition 0.000 description 8
- 241000048269 Pinus taiwanensis Species 0.000 description 8
- 235000013372 meat Nutrition 0.000 description 8
- 239000000203 mixture Substances 0.000 description 6
- 241001534110 Lactarius <percoid fish> Species 0.000 description 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 4
- 235000011613 Pinus brutia Nutrition 0.000 description 4
- 241000018646 Pinus brutia Species 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 4
- YOBAEOGBNPPUQV-UHFFFAOYSA-N iron;trihydrate Chemical compound O.O.O.[Fe].[Fe] YOBAEOGBNPPUQV-UHFFFAOYSA-N 0.000 description 4
- 230000009467 reduction Effects 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 244000191482 Cantharellus cibarius Species 0.000 description 1
- 235000015722 Cantharellus cibarius Nutrition 0.000 description 1
- 241000543691 Lactarius deliciosus Species 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A bionic cultivation method of purple mushroom comprises the steps of mycorrhizal seedling raising and field bionic cultivation. The purple mushroom (Lactarius vividus) is a fungus of Rugu of Russulaceae of Russula of Russulaceae, mainly distributed in the middle and south of China, and is a rare wild edible fungus. The strain has low natural yield and a descending trend, the price is always high, and the artificial cultivation is an urgent requirement at present. The invention discloses a bionic cultivation technology of the purple-flower fungi from the aspects of mycorrhizal seedling cultivation, field bionic cultivation, tree planting and afforestation in stony desertification areas and the like for the first time, and provides a new technology for stony desertification control, barren mountain afforestation, returning to farming and the like.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for cultivating ectomycorrhizal fungi and planting trees in the field, and more particularly relates to the technical problems of strain separation of purple-flowered fungi, mycorrhizal seedling synthesis and field planting trees.
Background
At present, most mycorrhizal fungi adopt a mode of cultivating mycorrhizal seedlings in an indoor sterile state and then cultivating soil, and the chanterelle (Canthraeus cibarius), the Lactarius deliciosus (Lactarius), the Lactarius hatsutake (Lactarius) and the like are successful. The purple mushroom (Lactarius viridus) is widely distributed in the middle and south of China and is eaten by a large number of people, in recent years, because the weather becomes warm, and human activities are added, the purple mushroom is difficult to find in mountain forests around cities, the trend of yield reduction is obvious, and no report about artificial bionic cultivation is provided at present, so that the yield of the purple mushroom can be increased and the vegetation can be recovered by combining bionic cultivation of the purple mushroom with afforestation, the ecological environment is improved, and the income is created for local farmers.
Disclosure of Invention
The invention aims to provide a bionic cultivation method of purple mushroom and application thereof in planting trees and forests. At present, no report about artificial bionic cultivation is available, and the bionic cultivation of the purple mushroom and the afforestation can increase the yield of the purple mushroom, restore vegetation, improve the ecological environment and create income for local farmers.
In order to achieve the above purpose of the present invention, the present invention provides the following technical solutions:
a bionic cultivation method of purple mushroom comprises the steps of mycorrhizal seedling raising and field bionic cultivation.
According to the bionic cultivation method of the purple mushroom, the mycorrhizal seedling raising step comprises the following steps:
1) preparing a host plant seedling: selecting host plant pinus massoniana seeds of purple fungi, cleaning the seeds for three times by using tap water, soaking the seeds for 30 minutes by using 75% alcohol for surface disinfection, flushing the seeds for three times by using sterile water, sowing the seeds in a plastic basket filled with 1:1 vermiculite and perlite, culturing the seeds under the conditions of accurately controlled greenhouse of 20-25 ℃ of air temperature and 60-70% of substrate humidity, irrigating the seeds by using sterile water, and preparing for inoculation after culturing the seeds in the host plant substrate for 3 months; sterilizing the seedling culture substrates vermiculite and perlite for 1h at 121 ℃ and 0.1MPa, standing for 3 days after sterilization, and sterilizing again for 1 time under the same conditions for use;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4.7H2O:1.5g/L,KH2PO4: 3g/L, yeast extract: 5g/L, glucose: 20g/L, VB1:0.01g/L,NaCl:0.058g/L,CaCl2: 1.11g/L, ferric citrate: 1.5X 10- 2g/L, agar: 20g/L, and culturing for 20 days to obtain mycelium.
3) Infection of host plants by the purple-flowered fungi: transferring the non-pollution mycelia of the purple mushrooms obtained by the separation in the step 2) into an MMN liquid culture medium, containing no agar, standing and culturing for 4 weeks at 28 ℃, dividing the mycelia into 1cm × 1cm squares under aseptic operation, taking out 1) culturing 3 half-and-half-month host seedlings, cutting off main root tips, wherein the height of a nutrition pot is 14cm, the bottom is 5cm × 5cm, and the top is 6.5cm × 6.5cm, putting the host seedlings into the pot, adding a substrate to 1/3 parts of the root length, putting 2) cultured mycelia into the pots, cutting the mycelia into 1cm × 1cm squares, putting 4 blocks of the nutrition pots close to the root, then continuously adding the substrate to 2cm of the pot mouth, adding 1:1 sterilized vermiculite and perlite as the substrate, and culturing for 10-11 months to obtain the plants infected by the purple mushrooms.
According to the bionic cultivation method of the purple fungi, in the preparation step of the host plant seedlings, the pinaceae plants are selected as host plants of the purple fungi.
According to the bionic cultivation method for the purple mushroom, the outdoor bionic cultivation refers to transplanting mycorrhizal seedlings, infected by the purple mushroom, which are cultivated for 12-18 months, into natural soil, wherein the soil is red soil, yellow soil and purple soil, and the pH value of the soil is 5-6.5.
According to the bionic cultivation method of the purple mushroom, the soil is slate development soil.
The bionic cultivation method of the purple mushroom is applied to plant afforestation, a plant infected by the purple mushroom cultured for 12-18 months, namely a mycorrhizal sapling, is transplanted into natural soil, purple mushroom fruiting bodies are harvested in 2-3 years, the soil is red soil, yellow soil and purple soil, and the pH value of the soil is 5-6.5; the mycorrhizal sapling is obtained by the following method:
1) preparing host plant seedlings: selecting host plant pinus massoniana seeds of purple fungi, cleaning the seeds for three times by using tap water, soaking the seeds for 30 minutes by using 75% alcohol for surface disinfection, flushing the seeds for three times by using sterile water, sowing the seeds in a plastic basket filled with 1:1 vermiculite and perlite, culturing the seeds under the conditions of accurately controlled greenhouse of 20-25 ℃ of air temperature and 60-70% of substrate humidity, irrigating the seeds by using sterile water, and preparing for inoculation after culturing the seeds in the host plant substrate for 3 months; sterilizing the seedling culture substrates vermiculite and perlite for 1h at 121 ℃ and 0.1MPa, standing for 3 days after sterilization, and sterilizing again for 1 time under the same conditions for use;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4.7H2O:1.5g/L,KH2PO4: 3g/L, yeast extract: 5g/L, glucose: 20g/L, VB1:0.01g/L,NaCl:0.058g/L,CaCl2: 1.11g/L, ferric citrate: 1.5X 10- 2g/L, agar: 20g/L, and culturing for 20 days to obtain mycelium.
3) Infection of host plants by the purple-flowered fungi: transferring the non-pollution mycelia of the purple mushrooms obtained by the separation in the step 2) into an MMN liquid culture medium, containing no agar, standing and culturing for 4 weeks at 28 ℃, dividing the mycelia into 1cm × 1cm squares under aseptic operation, taking out 1) culturing 3 half-and-half-month host seedlings, cutting off the tips of main roots, wherein the height of a nutrition pot is 14cm, the length of the bottom is 5cm × 5cm, the width of the top is 6.5cm × 6.5cm, putting the host seedlings into the pot, adding a substrate to 1/3 parts of the root length, putting the cultured mycelia into 1cm × 1cm squares, placing 4 blocks in each nutrition pot and closing to the root, then continuously adding the substrate to 2cm of the pot mouth, adding 1:1 sterilized vermiculite and perlite into the substrate, and culturing for 10-11 months to obtain the plants infected by the purple mushrooms.
The invention provides a bionic cultivation technology of the purple mushroom, and the technology is combined with afforestation, so that the yield of the purple mushroom is stable, the vegetation recovery is facilitated, the natural environment is friendly, and the local farmer is created.
Detailed Description
The following examples are provided to further illustrate the essence of the present invention and are not intended to limit the present invention.
Example 1
Bionic cultivation of purple mushroom (Lactarius vividus) and masson pine (Pinus massoniana) and application thereof in planting forests.
Identifying fruiting bodies of the purple mushrooms: the diameter of the pileus is 3-10cm, and when the pileus is rolled inwards, the center is quickly sunken and even turns outwards; the surface has slight annular stripe, water immersion, slight oily, light yellow, orange, gray orange, golden yellow, brown yellow, orange color of the mixture of central ochre and light gray green, orange color of the mixture of orange color and light blue green color of the mixture of orange color; some Basidion fruits are jagged at their edges when they are fully ripe, and occasionally split locally. The thickness of the mushroom meat is 2-8mm, the mushroom meat is light orange or lighter than the mushroom cap, the mushroom meat does not change color after being scratched, and the mushroom meat is light green after being aged. The fungus folds are 2-8mm wide and thick, are arranged crowded when young, are separated gradually along with time, and grow to a short and prolonged life, have bright orange color, golden yellow color and orange color, and do not fade to dark green and green when old or scratched. The stipe is 3-4.5 multiplied by 0.8-2cm, equally thick or gradually thinned downwards; the surface is slightly water-immersed, is slightly sunken, is bright orange to golden yellow, is almost the same as the color of a mushroom fold, has light local color and near white or lighter top, and does not change into green after being scratched; the bottom is provided with short, light orange or light green bristles. Less milk, orange to golden yellow, faded slowly to dark reddish blood after 2-10 minutes or drying. The spores were printed in light ochre.
1) Obtaining the pinus massoniana sterile seedlings: cleaning Pinus massoniana seeds with tap water for three times, soaking in 75% alcohol for 30 minutes for surface disinfection, performing aseptic water washing for three times, sowing in a plastic basket filled with 1:1 vermiculite and perlite, culturing under the conditions of a precisely controlled greenhouse of air temperature of 20-25 ℃ and matrix humidity of 60-70%, and performing aseptic water washing; sterilizing vermiculite and perlite as seedling culture medium at 121 deg.C under 0.1MPa for 1 hr, standing for 3 days, sterilizing again for 1 time under the same conditions, culturing in host plant matrix for 3 months, and preparing for inoculation;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4.7H2O:1.5g,KH2PO4: 3g, yeast extract: 5g, glucose: 20g, VB1:0.01g,NaCl:0.058g,CaCl2: 1.11g, ferric citrate: 1.5X 10-2g, agar: 20g (1L); after 20 days mycelium grew.
3) Inoculation: after 6 months of cultivation of the pinus massoniana seedlings, the pinus massoniana seedlings are ready for inoculation. Before inoculation, the root tip of the main root of the seedling needs to be cut off by 1-2cm so as to facilitate the growth and infection of the lateral root; after the purple mushroom mycelium flat plate is full, under the aseptic condition, dividing the mycelium into squares with the size of 1cm multiplied by 1cm for standby application by using an inoculating knife, putting host seedlings into bowls, adding a matrix to the position with the root length being about 1/3, putting cut mycelium blocks, putting 4 blocks in each nutrition bowl and enabling the nutrition bowls to be close to the root, then continuously adding the matrix to the position with the bowl mouth being 2cm, wherein the added matrix is sterilized vermiculite and perlite with the ratio of 1:1, and culturing for 10-11 months to obtain the Chinese red mushroom infected masson pine;
4) field transplanting: transplanting the pinus massoniana seedlings cultured for 12-18 months into a region suitable for the growth of pinus massoniana and purple mushroom, wherein the pH value of soil is 5-6.5, the development soil of slate is optimal, during the mushroom production period, a cloud precious region is 6 months to 8 months of rainy season, a large amount of rainfall and large amount of temperature reduction are generated, the maximum temperature is lower than 20 ℃, the minimum temperature is above 10 ℃, the ground temperature is about 10-18 ℃, the average rainfall is 6-8mm per day, and the relative humidity reaches 80%. And after 2-3 years, the purple-flower mushroom fruiting bodies can be harvested. Five years after transplantation, 569 purple-flowered sporocarp (30kg) were produced from 32 pine trees.
The field transplanting of the purple-flower mycorrhizal masson pine seedlings has the advantages that the survival rate and the growth rate of the pine seedlings are improved compared with those of nonmycorrhizal masson pine seedlings, the edible fungi cultivated in a forest plantation represent a novel agricultural production activity, the increasing demand on delicious food can be met, the natural ecosystem can be maintained, and extra environmental and social economic benefits can be provided.
Example 2
Bionic cultivation of purple mushroom (Lactarius vividus) and Taiwan pine (Pinus taiwanensis) and application thereof in planting trees and forests.
Identifying fruiting bodies of the purple mushrooms: the diameter of the pileus is 3-10cm, and when the pileus is rolled inwards, the center is quickly sunken and even turns outwards; the surface has slight annular stripe, water immersion, slight oily, light yellow, orange, gray orange, golden yellow, brown yellow, orange color of the mixture of central ochre and light gray green, orange color of the mixture of orange color and light blue green color of the mixture of orange color; some Basidion fruits are jagged at their edges when they are fully ripe, and occasionally split locally. The thickness of the mushroom meat is 2-8mm, the mushroom meat is light orange or lighter than the mushroom cap, the mushroom meat does not change color after being scratched, and the mushroom meat is light green after being aged. The fungus folds are 2-8mm wide and thick, are arranged crowded when young, are separated gradually along with time, and grow to a short and prolonged life, have bright orange color, golden yellow color and orange color, and do not fade to dark green and green when old or scratched. The stipe is 3-4.5 multiplied by 0.8-2cm, equally thick or gradually thinned downwards; the surface is slightly water-immersed, is slightly sunken, is bright orange to golden yellow, is almost the same as the color of a mushroom fold, has light local color and near white or lighter top, and does not change into green after being scratched; the bottom is provided with short, light orange or light green bristles. Less milk, orange to golden yellow, faded slowly to dark reddish blood after 2-10 minutes or drying. The spores were printed in light ochre.
1) Cleaning Taiwan pine seeds for three times by using tap water, soaking the seeds for 30 minutes by using 75% alcohol for surface disinfection, flushing the seeds for three times by using sterile water, sowing the seeds into a plastic basket filled with 1:1 vermiculite and perlite, culturing the seeds under the conditions that the air temperature is 20-25 ℃ and the substrate humidity is 60-70% and flushing the seeds by using the sterile water; sterilizing vermiculite and perlite as seedling culture medium at 121 deg.C under 0.1MPa for 1 hr, standing for 3 days, sterilizing again for 1 time under the same conditions, culturing in host plant matrix for 3 months, and preparing for inoculation;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4.7H2O:1.5g,KH2PO4: 3g, yeast extract: 5g, glucose: 20g, VB1:0.01g,NaCl:0.058g,CaCl2: 1.11g, ferric citrate: 1.5X 10-2g, agar: 20g (1L);
3) inoculation: the Taiwan pine is cultivated for 3 and a half months before being inoculated. Before inoculation, the root tip of the main root of the seedling needs to be cut off by 1-2cm so as to facilitate the growth and infection of the lateral root; after the purple mushroom mycelium flat plate is full, under the aseptic condition, dividing the mycelium into squares with the size of 1cm multiplied by 1cm for standby by using an inoculating knife, putting host seedlings into bowls, adding a matrix to the position with the root length of about 1/3, putting cut mycelium blocks, putting 4 blocks in each nutrition bowl and closing to the root, then continuously adding the matrix to the position with the bowl mouth of 2cm, wherein the added matrix is sterilized vermiculite and perlite with the ratio of 1:1, and culturing for 10-11 months to obtain Taiwan pine seedlings infected by the purple mushroom;
4) field transplanting: transplanting the seedling of the Taiwan pine cultivated for 10-11 months into a region suitable for the growth of the Taiwan pine and the purple mushroom, wherein the pH value of soil is 5-6.5, the development soil of slate is optimal, during the mushroom production period, the cloud precious region is 6 months to 8 months of rainy season, a large amount of rainfall and large amount of temperature reduction are generated, the maximum temperature is lower than 20 ℃, the minimum temperature is higher than 10 ℃, the ground temperature is about 10-18 ℃, the average rainfall is 6-8mm per day, and the relative humidity reaches 80%. And after 2-3 years, the purple-flower mushroom fruiting bodies can be harvested. And after 2-3 years, the purple-flower mushroom fruiting bodies can be harvested. After five years of transplantation, 593 purple-flower fungus sporophores (30kg) can be harvested from 40 pine.
The field transplantation of the purple-flower fungus mycorrhizal Taiwan pine not only improves the survival rate of pine seedlings, but also can harvest purple-flower fungus sporocarp, and is economic and ecological.
Claims (4)
1. A bionic cultivation method of purple mushroom Lactarius vividus is characterized in that the method comprises the steps of mycorrhizal seedling raising and field bionic cultivation;
the mycorrhizal seedling raising step comprises the following steps:
1) preparing a host plant seedling: selecting host plant pinus massoniana seeds of purple fungi, cleaning the seeds for three times by using tap water, soaking the seeds for 30 minutes by using 75% alcohol for surface disinfection, flushing the seeds for three times by using sterile water, sowing the seeds in a plastic basket filled with 1:1 vermiculite and perlite, culturing the seeds under the conditions of accurately controlled greenhouse of 20-25 ℃ of air temperature and 60-70% of substrate humidity, irrigating the seeds by using sterile water, and preparing for inoculation after culturing the seeds in a main plant substrate for 3 months; sterilizing the seedling culture substrates vermiculite and perlite for 1h at 121 ℃ and 0.1MPa, standing for 3 days after sterilization, and sterilizing again for 1 time under the same conditions for use;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4·7H2O:1.5g/L,KH2PO4: 3g/L, yeast extract: 5g/L, glucose: 20g/L, VB1:0.01g/L,NaCl:0.058g/L,CaCl2: 1.11g/L, ferric citrate: 1.5X 10- 2g/L, agar: 20g/L, culturing for 20 days to obtain mycelium;
3) infection of host plants by the purple-flowered fungi: transferring the non-pollution mycelia of the purple mushrooms obtained by the separation in the step 2) into an MMN liquid culture medium, containing no agar, standing and culturing for 4 weeks at 28 ℃, dividing the mycelia into 1cm × 1cm squares under aseptic operation, taking out 1) culturing 3 half-and-half-month host seedlings, cutting off main root tips, wherein the height of a nutrition pot is 14cm, the bottom is 5cm × 5cm, and the top is 6.5cm × 6.5cm, putting the host seedlings into the pot, adding a substrate to 1/3 parts of the root length, putting 2) cultured mycelia into the pots, cutting the mycelia into 1cm × 1cm squares, putting 4 blocks of the nutrition pots close to the root, then continuously adding the substrate to 2cm of the pot mouth, adding 1:1 sterilized vermiculite and perlite as the substrate, and culturing for 10-11 months to obtain the plants infected by the purple mushrooms.
2. The bionic cultivation method of purple mushroom according to claim 1, characterized in that the field bionic cultivation is that the mycorrhizal seedlings of the purple mushroom infected plants cultivated for 12-18 months are transplanted into natural soil, the soil is red soil, yellow soil or purple soil, and the pH value of the soil is 5-6.5.
3. The biomimetic culture method of purple mushroom according to claim 2, characterized in that the soil is slate development soil.
4. The application of the bionic cultivation method of purple mushroom in the plant forestation of claim 1, which is characterized in that the plant infected by the purple mushroom cultured for 12-18 months, namely mycorrhizal sapling, is transplanted into natural soil, and the purple mushroom fruiting bodies are harvested in 2-3 years, wherein the soil is red soil, yellow soil or purple soil, and the pH value of the soil is 5-6.5; the mycorrhizal sapling is obtained by the following method:
1) preparing host plant seedlings: selecting host plant pinus massoniana seeds of purple fungi, cleaning the seeds for three times by using tap water, soaking the seeds for 30 minutes by using 75% alcohol for surface disinfection, flushing the seeds for three times by using sterile water, sowing the seeds in a plastic basket filled with 1:1 vermiculite and perlite, culturing the seeds under the conditions of accurately controlled greenhouse of 20-25 ℃ of air temperature and 60-70% of substrate humidity, irrigating the seeds by using sterile water, and preparing for inoculation after culturing the seeds in the host plant substrate for 3 months; sterilizing the seedling culture substrates vermiculite and perlite for 1h at 121 ℃ and 0.1MPa, standing for 3 days after sterilization, and sterilizing again for 1 time under the same conditions for use;
2) obtaining the strains of the purple fungi: under the aseptic condition, taking the flesh tissue at the joint of the pileus and the stipe of the purple mushroom, inoculating the flesh tissue on an MMN culture medium, and culturing in a dark place at 28 ℃, wherein the MMN culture medium comprises the following formula: MgSO (MgSO)4·7H2O:1.5g/L,KH2PO4: 3g/L, yeast extract: 5g/L, glucose: 20g/L, VB1:0.01g/L,NaCl:0.058g/L,CaCl2: 1.11g/L, ferric citrate: 1.5X 10- 2g/L, agar: 20g/L, culturing for 20 days to obtain mycelium;
3) infection of host plants by the purple-flowered fungi: transferring the non-pollution mycelia of the purple mushrooms obtained by the separation in the step 2) into an MMN liquid culture medium, containing no agar, standing and culturing for 4 weeks at 28 ℃, dividing the mycelia into 1cm × 1cm squares under aseptic operation, taking out 1) culturing 3 half-and-half-month host seedlings, cutting off the tips of main roots, wherein the height of a nutrition pot is 14cm, the length of the bottom is 5cm × 5cm, the width of the top is 6.5cm × 6.5cm, putting the host seedlings into the pot, adding a substrate to 1/3 parts of the root length, putting the cultured mycelia into 1cm × 1cm squares, placing 4 blocks in each nutrition pot and closing to the root, then continuously adding the substrate to 2cm of the pot mouth, adding 1:1 sterilized vermiculite and perlite into the substrate, and culturing for 10-11 months to obtain the plants infected by the purple mushrooms.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610707414.7A CN106305138B (en) | 2016-08-23 | 2016-08-23 | Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610707414.7A CN106305138B (en) | 2016-08-23 | 2016-08-23 | Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106305138A CN106305138A (en) | 2017-01-11 |
CN106305138B true CN106305138B (en) | 2020-02-21 |
Family
ID=57741721
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610707414.7A Active CN106305138B (en) | 2016-08-23 | 2016-08-23 | Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106305138B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116034812A (en) * | 2023-02-28 | 2023-05-02 | 钦州市林业科学研究所 | Method for newly planted rhodozyma subartificial cultivation of rhodozyma under forest |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1240572A (en) * | 1998-06-25 | 2000-01-12 | 谭著明 | Culture medium of pine lactarius strains and artificial cultivation method for pine lactarius |
CN101953273A (en) * | 2010-11-01 | 2011-01-26 | 湖南省林业科学院 | Production increasing method for bolete in forests |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101595803B (en) * | 2008-06-03 | 2011-04-13 | 上海市农业科学院 | Method for producing edible fungus mycorrhizal seedling of mycorhiza |
-
2016
- 2016-08-23 CN CN201610707414.7A patent/CN106305138B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1240572A (en) * | 1998-06-25 | 2000-01-12 | 谭著明 | Culture medium of pine lactarius strains and artificial cultivation method for pine lactarius |
CN101953273A (en) * | 2010-11-01 | 2011-01-26 | 湖南省林业科学院 | Production increasing method for bolete in forests |
Non-Patent Citations (3)
Title |
---|
光黑腹菌及其半人工模拟合成研究;陈羽等;《食用菌学报》;19990228;第6卷(第1期);第23页 * |
外生菌根真菌菌株耐盐碱性筛选以及黑松(Pinus thunbergii Parl.)菌根苗合成、耐盐碱性研究;李小利;《中国优秀硕士学位论文全文数据库农业科技辑》;20140831(第8期);第17-19页 * |
陈羽等.光黑腹菌及其半人工模拟合成研究.《食用菌学报》.1999,第6卷(第1期), * |
Also Published As
Publication number | Publication date |
---|---|
CN106305138A (en) | 2017-01-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106613324A (en) | Ganoderma undergrowth bionics wild cultivation method | |
CN107711290B (en) | Culture medium for mycorrhizal edible fungus symbiotic seedling and synchronous culture method thereof | |
CN103125244A (en) | Cutting propagation method of golden camellia plants | |
CN103609441B (en) | A kind of blueberry seedling-cultivating method | |
CN104920212A (en) | Siraitia grosvenorii tissue culture seedling propagation method | |
CN105191727A (en) | Dragon fruit planting method | |
CN104186265B (en) | A kind of blueberry list trunk Labor-saving cultivation shaping methods | |
KR101106961B1 (en) | In vitro germination method of Gastrodia elata seeds using symbiotic fungal species | |
CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
CN102754596A (en) | Establishing method of symbiont of cyclobalanopsis glaucoides and bolete | |
CN112273228A (en) | Cultivation method of Yunnan pine mycorrhizal seedlings | |
CN106386329A (en) | High-yield early-fruiting cultivation method for Jizui litchi | |
CN109757274B (en) | High-yield and high-efficiency transplanting method for momordica grosvenori tissue culture seedlings | |
CN108243832A (en) | The artificial method for planting of paint face mushroom | |
CN101385440B (en) | Quick propagation method of Tectona grandis | |
CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
CN106718496A (en) | A kind of method that old kind camellia oleifera lam reconstruction of mountainous region of low yield improves yield | |
CN105941154A (en) | Comprehensive breeding method for superior winter jujube seedlings | |
CN104641879A (en) | Method for planting dendrobium candidum in metasequoia tree forest | |
CN104303640A (en) | Seedling breeding method of cassava | |
CN106305138B (en) | Bionic cultivation method of purple mushroom and application of bionic cultivation method in planting trees and forests | |
CN105145269A (en) | Efficient seedling cultivating method for leafbuds of Yunshan 2# grape novel variety | |
CN109220478A (en) | A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf | |
CN104488721A (en) | Quick propagation method for tissue culture of hottonia inflata | |
CN114431013A (en) | Planting method of Venus golden apple trees |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
OL01 | Intention to license declared | ||
OL01 | Intention to license declared |