CN106289908B - Cell centrifuge and cell centrifugation method - Google Patents
Cell centrifuge and cell centrifugation method Download PDFInfo
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- CN106289908B CN106289908B CN201610680928.8A CN201610680928A CN106289908B CN 106289908 B CN106289908 B CN 106289908B CN 201610680928 A CN201610680928 A CN 201610680928A CN 106289908 B CN106289908 B CN 106289908B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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Abstract
The invention relates to cell centrifuges and cell centrifuging methods, the cell centrifuges include the cantilever, centrifugal rotary table used for placing the centrifugal storehouse and sample rotary table used for placing the sample bottle, the sample rotary table is located in the periphery of centrifugal rotary table, the cantilever locates above centrifugal rotary table and sample rotary table and can move horizontally, there is a sampling mechanism on the said cantilever, and the said sampling mechanism can move up and down relatively to the cantilever, the sampling mechanism sucks the sample in the sample bottle, move horizontally to the centrifugal rotary table above and drip the sample to the centrifugal storehouse, finish times of transfer operations of the sample, after finishing pipetting, the centrifugal rotary table centrifugates the sample in the centrifugal storehouse, realize the automatic centrifugal treatment of the cell.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to an cell centrifuge and a cell centrifugation method.
Background
The method comprises the steps of firstly sampling, then carrying out centrifugal operation on a sample for a specific time and times by using a centrifugal machine, discarding supernatant containing mucus, red cell debris and other necrotic cell components, then adding cell adhesion wrapping liquid, finally manufacturing a thin-layer cell smear with monolayer cell distribution, and then manually staining, wherein generally needs to carry out centrifugal separation on cells manually, and the supernatant containing the mucus, red cell debris and other necrotic cell components is discarded, the previous treatments are usually carried out manually, the loss of diagnostic cells is easily caused by artificial mistake, and the safety protection is poor because an operator directly contacts with a specimen.
Disclosure of Invention
Therefore, there is a need to provide cytocentrifuges and cytocentrifugation methods for realizing automatic centrifugation of cells and avoiding cell loss or contamination caused by manual contact with samples, which are directed to the defects of the prior art.
The technical scheme is as follows:
kinds of cell centrifuge, including the cantilever, be used for placing the centrifugation carousel in centrifugation storehouse and be used for placing the sample carousel of sample bottle, the sample carousel is located centrifugation carousel periphery, but the cantilever is located centrifugation carousel and sample carousel top and horizontal migration, be equipped with sampling mechanism on the cantilever, just sampling mechanism can reciprocate by relative cantilever.
The technical scheme of is as follows:
the centrifugal rotary table is driven by an th driving mechanism, the th driving mechanism comprises a centrifugal motor, an electromagnetic clutch and a th positioning motor, the centrifugal motor is connected with the transmission shaft of the centrifugal rotary table, and the th positioning motor is connected with the transmission shaft of the centrifugal rotary table through the electromagnetic clutch.
The sample carousel passes through the drive of second actuating mechanism, second actuating mechanism includes second positioning motor, hold-in range and gear, the gear with the output shaft of second positioning motor, the hold-in range cover is established outside the sample carousel, just be equipped with on the hold-in range outer wall with gear engagement's tooth.
The cell centrifuge also comprises a control mechanism, wherein an th positioning switch is arranged on the centrifugal turntable, a second positioning switch is arranged on the sample turntable, and the th positioning motor, the second positioning motor, the th positioning switch and the second positioning switch are respectively electrically connected with the control mechanism.
The sampling mechanism comprises a pipe frame and a sampling pipe arranged on the pipe frame, an th driving motor and a longitudinally-arranged transmission belt are arranged on the cantilever, the end of the transmission belt is rotationally connected with the rotating shaft of the th driving motor, the other end of the transmission belt is wound on a belt wheel, and the pipe frame is connected with the side of the transmission belt.
The sampling mechanism further comprises a pipette, the pipette is installed on the pipe frame, and the pipette and the sampling pipe are arranged at intervals in the front-back direction of the cantilever moving direction.
Cell centrifuge still include screw capping mechanism, screw capping mechanism sets up the th side at the cantilever, sampling mechanism sets up the second side at the cantilever, the long through-hole has been seted up to the position department that the cantilever is relative with the drive belt, screw capping mechanism passes through the long through-hole with the drive belt is connected, and the side that screw capping mechanism and drive belt are connected with the side that sampling mechanism and drive belt are connected sets up relatively, makes screw capping mechanism and sampling mechanism move towards different directions respectively along with the removal of drive belt.
And a plurality of sample grades for placing sample bottles, a plurality of sampling positions for placing sampling tubes and a plurality of avoidance positions for placing pipette tubes are arranged on the sample turntable around the circumferential direction of the sample turntable, and the sample grades, the sampling positions and the avoidance positions are positioned on the circumferential direction of the sample turntable with different radiuses.
The centrifugal rotary disc is provided with a plurality of clamping positions in the circumferential direction, the centrifugal bin is detachably connected with the clamping positions and can rotate around the clamping positions.
The cell centrifuge still include the workstation, sample carousel, centrifugal carousel rotationally set up on the workstation, be equipped with the support frame on the workstation, be equipped with the crossbeam of arranging along sample carousel or centrifugal carousel radial direction on the support frame, the cantilever passes through second driving motor control slidable ground and sets up on the crossbeam, be equipped with two on the crossbeam the cantilever.
And two screwing cover mechanisms, two sampling tubes and two liquid suction tubes are arranged on each cantilever, the two screwing cover mechanisms are arranged in parallel, the two sampling tubes are arranged in parallel, the two liquid suction tubes are arranged in parallel, and the parallel distance between the two screwing cover mechanisms, the two sampling tubes and the two liquid suction tubes is equal to the distance between adjacent sample bottles on the sample turntable.
Cell centrifuge still including the scanner that is located sample carousel and centrifugation carousel top, the scanner is used for scanning bar code on the centrifugation storehouse and/or bar code on the sample bottle and/or the bar code on the centrifuging tube in the centrifugation storehouse, the scanner with control mechanism electric connection.
Cell centrifuge still including the protector that is used for detecting centrifugal motor rotational speed, the centrifugation carousel is equipped with the safety cover outward, works as the protector detects the centrifugal motor is when running, the safety cover can't be opened just electromagnetic clutch can't move.
The cell centrifuge also comprises a balance detector used for detecting whether the left and the right of the sample to be centrifuged on the centrifugal turntable are balanced, and when the left and the right of the sample to be centrifuged on the centrifugal turntable are not balanced, the balance detector sends out warning and reminding.
The device comprises a cantilever, a th side of the cantilever is provided with a fixing frame connected with a transmission belt, a screwing mechanism comprises a third driving motor, a connecting shaft, a shell, a connecting piece, a connecting rod, a torque adjusting piece, a disc spring, a motion block, a one-way bearing and a cover body matched with a sample bottle cover, the end of the connecting shaft is connected with an output shaft of the third driving motor, the end of the connecting shaft is connected with the shell, the third driving motor is installed on the fixing frame, the connecting shaft is rotatably connected with the fixing frame, the shell is provided with a longitudinal sliding chute, the connecting piece is provided with an extension rod, the connecting piece is installed in the shell, the extension rod is in sliding fit with the sliding chute, the shell is internally provided with an elastic piece abutted against the upper part of the connecting piece, the one-way bearing is arranged in the connecting piece, the one-way bearing is sleeved outside the connecting rod, the outer ring of the one-way bearing is connected with the connecting piece, the torque adjusting piece is in threaded connection with the connecting piece, the upper end of the torque adjusting piece is provided with an extension part, the extension hole is formed in the extension part, the disc spring and the motion block and the inner wall of the disc spring is arranged in the torque adjusting piece, the upper end of the sample bottle cover body, the lower end face of the sample bottle cover body is provided with a plurality of the sample bottle cover body, the lower end of the sample bottle.
A method for centrifuging cells, comprising the steps of:
a. the centrifugal turntable and the sample turntable positioned on the periphery of the centrifugal turntable rotate to initial positions, so that the positions of the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins in the circumferential direction of the centrifugal turntable;
b. the cantilever drives the sampling mechanism on the cantilever to move to the upper part of the sample turntable, the sampling mechanism descends to absorb the sample in the sample bottle, the cantilever moves again, the sampling mechanism moves to the upper part of the centrifugal turntable, which corresponds to the current sample bottle, of the centrifugal bin, and the sampling mechanism descends to drop the sample into the centrifugal tube in the centrifugal bin;
c. the centrifugal turntable and the sample turntable respectively rotate for a specific angle, so that the positions of the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins in the circumferential direction of the centrifugal turntable, and the sample bottles to be processed and the centrifugal tubes in the centrifugal bins are positioned in the range of the cantilever movement stroke;
d. repeating the step b and the step c until the sample on the sample turntable is transferred;
e. and starting the centrifugal turntable to perform centrifugal treatment.
The technical scheme of is as follows:
in the cell centrifugation method, the step e specifically comprises the following steps:
e1, stopping after centrifuging the centrifugal turntable for a preset time, moving the cantilever above the centrifugal turntable, and descending the sampling mechanism to suck the supernatant in the centrifugal tube;
e2, restarting the centrifugal turntable for centrifugation;
e3, repeating the steps e1 and e2 until the expected centrifugal effect is achieved.
In the cell centrifugation method, in the step c, multiple rows of centrifuge tubes for placing samples are arranged in each centrifugal bin, after rows of centrifuge tubes are dripped with samples, the sample turntable rotates for a preset angle, the centrifugal turntable does not rotate, a sampling mechanism on the cantilever sucks the samples in sample bottles at corresponding positions, the cantilever moves to drip the samples into another rows of centrifuge tubes in the centrifugal bin, the centrifugal turntable rotates until the samples are dripped into the centrifuge tubes in the current centrifugal bin, and another centrifugal bin rotates to the range of the cantilever moving stroke.
According to the cell centrifugation method, when the centrifugation turntable is aligned with the sample turntable, an th positioning motor is adopted to drive the centrifugation turntable to rotate, and the th positioning motor is connected with a transmission shaft of the centrifugation turntable through an electromagnetic clutch;
the centrifugal turntable is driven by a centrifugal motor during centrifugal treatment, the electromagnetic clutch is disconnected with a transmission shaft of the centrifugal turntable at the moment, the centrifugal turntable is stopped after being driven by the centrifugal motor for a preset time, the electromagnetic clutch is connected with the transmission shaft of the centrifugal turntable again, an th positioning motor drives the centrifugal turntable to rotate so as to align the centrifugal turntable with a sampling mechanism, and after the sampling mechanism sucks supernatant in a centrifugal tube, the electromagnetic clutch is disconnected with the transmission shaft of the centrifugal turntable again, and the centrifugal motor drives the centrifugal turntable again to perform centrifugal treatment.
In the cell centrifugation method, when the sample bottle on the sample turntable is a sample bottle with a cover tightly covered, before sampling by the sampling mechanism, the cantilever drives the cover screwing mechanism on the cantilever to move to the upper part of the sample turntable, and after the cover screwing mechanism descends and unscrews the cover of the sample bottle at the corresponding position, the step b is carried out; and c, after the step b is finished, moving the cantilever above the sample turntable again, descending the cover screwing mechanism to screw down the uncapped sample bottle cover, and then executing the step c.
The advantages or principles of the foregoing technical solution are explained below:
the cell centrifuge and the cell centrifugation method have the advantages that the sample rotating disc is arranged on the periphery of the centrifugal rotating disc, the cantilever moves horizontally, the sampling mechanism absorbs samples in the sample bottles, then the samples are horizontally moved to the upper portion of the centrifugal rotating disc to be dripped into the centrifugal bin, times of sample transfer operation is completed, times of sample pipetting operation is completed, the sample rotating disc and/or the centrifugal rotating disc rotate, the sample bottles to be processed and the centrifugal bin are rotated to the moving stroke range of the cantilever, the cantilever only needs to move along directions, the pipetting speed is high, the sampling mechanism only conducts sampling operation on the sample bottles at the current position at each pipetting operation, other samples cannot be polluted, after pipetting is completed, the centrifugal rotating disc centrifuges the samples in the centrifugal bin, manual operation is not needed, the efficiency is higher, automatic cell centrifugation processing is achieved, and cell loss or contamination caused by manual contact of the samples is avoided.
Drawings
FIG. 1 is a schematic representation of a cytospin according to an embodiment of the present invention;
FIG. 2 is a second schematic diagram of a cytospin according to an embodiment of the present invention;
FIG. 3 is a partial schematic view of a cytospin according to an embodiment of the present invention after partially hiding parts;
FIG. 4 is a third schematic view of a cytospin according to an embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view of a screw capping mechanism according to an embodiment of the present invention;
FIG. 6 is an enlarged partial schematic view of FIG. 5;
FIG. 7 is a schematic view of the capping mechanism of the present invention with some parts hidden;
fig. 8 is a schematic view of the screw capping mechanism according to the embodiment of the present invention after hiding parts.
Description of reference numerals:
10. cantilever, 110, fixing frame, 120, driving motor, 130, driving belt, 140, belt wheel, 150, long through hole, 20, centrifugal turntable, 210, centrifugal bin, 212, centrifuge tube, 220, clamping position, 30, sample turntable, 310, sample bottle, 312, clamping table, 320, sample grade, 330, sampling position, 340, avoiding position, 40, screw cover mechanism, 410, third driving motor, 412, connecting shaft, 420, housing, 422, chute, 424, elastic element, 430, connecting element, 432, extension rod, 434, step position, 440, connecting rod, 442, ball, 444, linear bearing, 450, cover, 452, accommodating groove, 454, clamping groove, 460, torque adjusting element, 462, extension part, 464, adjusting hole, 470, disc spring, 480, moving block, 482, matching part, transmission part, 490, one-way bearing, 50, sampling mechanism, 510, 520, pipette, 530, extension part, 60, driving mechanism, 610, electric motor, 620, electromagnetic clutch, 730, positioning motor, rack, 810, positioning motor, bracket, 60, bracket, ultrasonic motor, ultrasonic sensor.
Detailed Description
As shown in fig. 1 to 4, kinds of cytospin comprises a cantilever 10, a centrifugal turntable 20 for placing a centrifugal bin 210, and a sample turntable 30 for placing a sample bottle 310, wherein the sample turntable is located at the periphery of the centrifugal turntable 20, the cantilever 10 is located above the centrifugal turntable 20 and the sample turntable and can move horizontally, a screw-cap mechanism 40 and a sampling mechanism 50 are arranged on the cantilever 10, and the screw-cap mechanism 40 and the sampling mechanism 50 can move up and down relative to the cantilever 10.
The sample rotating disc is arranged at the periphery of the centrifugal rotating disc 20, the cantilever 10 moves horizontally, the screw-capping mechanism 40 unscrews a sample bottle cover on the sample rotating disc, the sampling mechanism 50 sucks a sample in the sample bottle, the sample is horizontally moved above the centrifugal rotating disc 20 to drip the sample into the centrifugal bin 210, the cantilever 10 moves above the sample bottle after being opened, the screw-capping mechanism 40 screws the cover tightly, times of sample transfer operation is completed, each time times of sample pipetting operation is completed, the sample rotating disc and/or the centrifugal rotating disc 20 rotates the sample bottle to be processed and the centrifugal bin 210 to the moving stroke range of the cantilever 10, the cantilever 10 only needs to move along directions, the pipetting speed is high, and each time of pipetting operation, the screw-capping mechanism 40 and the sampling mechanism 50 only carry out screw-capping and sampling operation on the sample bottle at the current position, other samples cannot be polluted, after the centrifugal rotating disc 20 centrifugalizes the sample in the centrifugal bin 210, the cantilever 10 moves the supernatant of the sampling mechanism 50 every period, the cantilever 10 does not need to manually move the centrifugal bin 210, the supernatant liquid of the centrifugal rotating disc can be used for realizing higher cell handling efficiency, the sample bottle can be automatically treated, the sample bottle can be taken without the sample bottle being manually, the sample bottle is taken, the sample bottle is not required to be taken, and the sample bottle is not required to be taken, the sample bottle is taken after the sample bottle is taken, the.
As shown in fig. 4, the centrifugal turntable 20 is driven 60 by an th driving mechanism, the th driving mechanism includes a centrifugal motor 610, an electromagnetic clutch 620 and a th positioning motor 630, the centrifugal motor 610 is connected with a transmission shaft of the centrifugal turntable 20, the th positioning motor 630 is connected with the transmission shaft of the centrifugal turntable 20 by the electromagnetic clutch 620, the th positioning motor 630 is a stepping motor, the centrifugal motor 610 is a brushless motor, because the rotation speed required by the centrifugal process is large, and the centrifugal turntable 20 needs to be accurately positioned in the pipetting process, motors cannot simultaneously meet the requirements of the two motors, by arranging the th driving mechanism, when centrifuging, the centrifugal motor 610 works, the centrifugal turntable 20 runs at a high speed, the electromagnetic clutch 620 is disconnected with the transmission shaft of the centrifugal turntable 20, when positioning operation is required, the centrifugal motor 610 does not work, the electromagnetic clutch 620 is connected with the transmission shaft of the centrifugal turntable 20, and the th positioning motor 630 drives the centrifugal turntable 20 to run, so as to realize accurate positioning.
As shown in fig. 3, the sample turntable is driven by a second driving mechanism 70, the second driving mechanism includes a second positioning motor 710, a synchronous belt 720 and a gear 730, the gear 730 is connected to an output shaft of the second positioning motor 710, the synchronous belt 720 is sleeved outside the sample turntable, and teeth meshed with the gear 730 are arranged on an outer wall of the synchronous belt 720. Through setting up toothed hold-in range 720 and gear 730 meshing, second positioning motor 710 drive gear 730 operates, drives hold-in range 720 and sample carousel and rotates, can set up bearing or straining device on the hold-in range 720 and realize the regulation of hold-in range 720 elasticity.
Cell centrifuge still includes control mechanism, be equipped with positioning switch on the centrifugal carousel, be equipped with second positioning switch on the sample carousel, positioning motor, second positioning motor, 0 positioning switch and second positioning switch respectively with control mechanism electric connection 1 positioning motor operation of control mechanism control, it rotates to drive centrifugal carousel, control mechanism control second positioning motor operation, it rotates to drive the sample carousel, the centrifuging tube in centrifugal storehouse on the centrifugal carousel is 2 corresponding with the sample bottle on the sample carousel, move the liquid in-process, fix a position motor drive centrifugal carousel through the and rotate fixed angle second positioning motor drive sample carousel and rotate fixed angle, centrifuging tube on the centrifugal carousel behind the rotation angle and the sample bottle on the sample carousel do not only want the position to correspond, and still must correspond with the sampling mechanism position on the cantilever, ensure errorless, can realize the accurate location of centrifugal carousel with the sample carousel position through positioning switch and second positioning switch, advance step ensure the accuracy of liquid-moving carousel positioning switch, the second positioning switch is the light location, the initial position is just for a light transmission and the light transmission and light transmission is blocked when the initial location is determined, then.
As shown in fig. 1-5, the screw cap mechanism 40 is disposed on the side of the cantilever 10, the sampling mechanism 50 is disposed on the second side of the cantilever 10, the sampling mechanism 50 includes a tube rack 510, a sampling tube 520 and a pipette 530, the sampling tube 520 and the pipette 530 are disposed on the tube rack 510, and the pipette 530 and the sampling tube 520 are disposed at intervals in the movement direction of the cantilever 10, a 0 driving motor 120 is fixed on the side of the cantilever 10, a longitudinally disposed belt 130 is disposed on the second side of the cantilever 10, a end of the belt 130 is rotatably connected to a rotating shaft of the second pipette driving motor 120, another end of the pipette 130 is wound on a pulley 140, a long through hole 150 is formed at a position where the cantilever 10 is opposite to the belt 130, the screw cap mechanism 40 is connected to a side of the belt 130 through the long through hole 150, the sampling mechanism 50 is connected to another side of the belt 130, and the sampling mechanism 50 is connected to the other side of the cantilever 130, and the centrifugal cap mechanism 50 is connected to the sampling tube 130 through a centrifugal belt mechanism 50 and a centrifugal cap mechanism 130, the sampling tube 130, the sampling mechanism 50 is moved back and forth and back mechanisms 40, the sampling tube 50 are moved along the centrifugal cap mechanism 130, the sampling tube 130, the sampling mechanism 130, the sampling tube 50 and forth movement direction is not interfered with the sampling mechanism 130, and back movement mechanism 130, and forth movement mechanism 130, the sampling tube 130, the sampling mechanism 130, the sampling tube 50 is not only, the sampling tube 130 is moved along the sampling tube 50, and forth movement direction is not interfered with the sampling mechanism 130, and back movement mechanism 130, and the centrifugal cap mechanism 130, the sampling tube 130, the sampling mechanism 130.
The sample turntable is provided with a plurality of sample positions 320 for placing sample bottles, a plurality of sampling positions 330 for placing sampling tubes 520 and a plurality of avoidance positions 340 for avoiding the pipette tubes 530 in the circumferential direction, and the sample positions 320, the sampling positions 330 and the avoidance positions 340 are positioned on the circumference of the sample turntable at different radiuses.
The centrifugal turntable 20 is provided with a plurality of clamping positions 220 along the circumferential direction, the centrifugal bin 210 is detachably connected with the clamping positions 220, and the centrifugal bin 210 can rotate around the clamping positions 220. Make centrifugation storehouse 210 can be on centrifugal carousel 20 through screens 220, can directly take off centrifugation storehouse 210 after the sample centrifugation and move to dyeing pelleter on, need not to shift respectively to single sample, and easy and simple to handle is swift, and centrifugation storehouse 210 can rotate around screens 220, the change of the centrifugation storehouse 210 position in the centrifugal process of being convenient for.
The cell centrifuge further comprises a workbench 80, the sample turntable and the centrifugal turntable 20 are rotatably arranged on the workbench 80, a support frame 810 is arranged on the workbench 80, a cross beam 820 arranged along the radial direction of the sample turntable or the centrifugal turntable 20 is arranged on the support frame 810, the cantilever 10 is slidably arranged on the cross beam 820 under the control of a second driving motor 830, and two cantilevers 10 are arranged on the cross beam 820. The two cantilevers 10 are arranged, so that the pipetting and liquid absorbing efficiency can be improved, the cantilevers 10 move along the radial direction of the sample turntable or the centrifugal turntable 20, and therefore the positions of the sample bottles and the centrifugal bins 210 on the sample turntable or the centrifugal turntable 20 after rotation can be located in the moving stroke of the cantilevers 10, and the alignment is more accurate.
Each cantilever 10 is provided with two capping mechanisms 40, two sampling tubes 520 and two pipette tubes 530, the two capping mechanisms 40 are arranged in parallel, the two sampling tubes 520 are arranged in parallel, the two pipette tubes 530 are arranged in parallel, and the parallel distance between the two capping mechanisms 40, the two sampling tubes 520 and the two pipette tubes 530 is equal to the distance between adjacent sample bottles on the sample turntable. Set up like this, when operation at every turn, screw capping mechanism 40 can carry out the screw capping to two sample bottles simultaneously, and sampling mechanism 50 can get liquid and imbibition to two samples simultaneously, and efficiency is higher.
Cell centrifuge still include control mechanism and be located the scanner of sample carousel and centrifugal carousel 20 top, the scanner is used for scanning bar code on the centrifugation storehouse 210 and/or the bar code on the sample bottle and/or the bar code on centrifuging tube 212 in the storehouse 210, the scanner with control mechanism electric connection, control mechanism with screw cap mechanism 40, sampling mechanism 50 electric connection. The corresponding bar codes are scanned by the scanner and fed back to the control mechanism, so that the sample bottles can be more accurately corresponding to the centrifugal tubes 212 of the centrifugal bin 210, and the sample is prevented from being mistaken.
Cell centrifuge still including the protector that is used for detecting centrifugal motor 610 rotational speed, centrifugal carousel 20 is equipped with the safety cover outward, works as the protector detects centrifugal motor 610 is when running, the safety cover can't open just electromagnetic clutch 620 can't move, the safety cover is closed during centrifugation, avoids the sample to splash etc. causes personnel's injury, only detects centrifugal motor 610 when stopping the back when the protector, the safety cover just can normally open, electromagnetic clutch 620 can normal operating, passes positioning motor 630's power to centrifugal carousel 20.
The cytospin still including be used for detecting on centrifugal carousel 20 treat whether balanced detector about the centrifugal sample, when detecting on centrifugal carousel 20 treat that centrifugal sample is uneven about, the equilibrium detector sends and warns and reminds, centrifugal carousel 20 rotational speed is fast during centrifugal operation, if the left and right sides is uneven, causes the rotation unstability easily, and the sample flies out the scheduling problem, through setting up the equilibrium detector, denier detects the unbalance and in time sends out the warning and adjusts, avoids the dangerous condition to appear.
As shown in fig. 5-8, the screw-on mechanism 40 includes a third driving motor 410, a housing 420, a connecting member 430, a connecting rod 440, and a cover 450 for engaging with a cap of a sample bottle, the housing 420 is provided with a sliding slot 422 along a longitudinal direction, the connecting member 430 is provided with an extending rod 432, the connecting member 430 is installed in the housing 420, the extending rod 432 is slidably engaged with the sliding slot 422, the housing 420 is provided with an elastic member 424 abutting on the connecting member 430, the third driving motor 410 is in transmission connection with the housing 420, an end of the connecting rod 440 extends into the housing 420 to be connected with the connecting member 430, an end of the connecting rod 440 is connected with the cover 450, the third driving motor 410 rotates to drive the housing 420 connected therewith to rotate, thereby driving the connecting member 430 to rotate, the connecting rod 440 and the cover 450 rotate, the cover 450 is provided with the cap to drive the cap to rotate, thereby enabling the cap to unscrew or screw down the cap, the cap 450 is provided with a plurality of clamping jaws 452 for engaging with the cap cover 450, and the clamping jaws 450 are provided with a plurality of clamping jaws 454 capable of engaging with the clamping jaws 454 to be inserted into the clamping slots 450, when the cap 220, the cap is inserted and the cap 220, the cap is not to be inserted into a plurality of the clamping jaws 454, and the clamping jaws 450, so that the clamping jaws 450, the cap 220, when the cap is inserted and the cap 220, the cap is inserted with the clamping jaws 454, the cap 220, the cover 220, the clamping jaws 454, the cover 220, the cover is inserted cap is inserted and the cover 220, the cover is inserted and the cover is inserted in a mode that the cover is not to be able to be inserted in a mode that the cover is inserted in a plurality of the mode that is not to be inserted mode that is inserted and the cover 220, and the cover 220.
, to avoid the damage of the cover or the damage of the third driving motor 410 caused by the over-large torque during the cover screwing process, the screwing mechanism 40 further includes a torque adjusting member 460, a disc spring 470, a moving block 480 and a one-way bearing 490, the torque adjusting member 460 is connected to the connecting member 430, the one-way bearing 490 is disposed in the connecting member 430, the one-way bearing 490 is sleeved outside the connecting rod 440, the outer ring of the one-way bearing 490 is connected to the connecting member 430, the disc spring 470 and the moving block 480 are disposed in the torque adjusting member 460, the end of the disc spring 470 abuts against the inner wall of the torque adjusting member 460, the end abuts against the upper end of the moving block 480, the lower end of the moving block 480 is engaged with the upper end of the connecting rod 440 through the balls 442, the one-way bearing 490 is a fixed outer ring, the inner ring can only rotate in the cover screwing direction, when the cover is unscrewed, the torque of the third driving motor 410 is transmitted to the housing 420, the connecting member 430 and the one-way bearing 490 connected to the connecting rod 430, the torque adjusting member 480 is transmitted to the connecting rod 480 and the connecting rod 430, the disc spring 480 is prevented from rotating relative to the outer ring, the connecting rod 480 and the connecting rod 440, the cover 480 is directly transmitted to the connecting rod 480 and the connecting rod 440, the connecting rod 450, the cover 450, the third driving mechanism is further operated, the disc spring works, the disc spring 480 is moved to the disc spring 480 and the cover 480 to prevent the disc spring from slipping.
The motion block 480 of the embodiment includes a matching portion 482 and a transmission portion 484, a lower end of the matching portion 482 is connected with an upper end of the transmission portion 484 to form a T shape, a limit hole is formed at an upper end of the torque adjuster 460, the matching portion 482 is in sliding fit with the limit hole, the disc spring 470 is sleeved outside the matching portion 482, the disc spring 470 abuts against the transmission portion 484, a -th groove is formed on a lower end surface of the transmission portion 484, a second groove is formed on an upper end surface of the connection rod 440, the balls 442 are located in a space formed by butting the -th groove and the second groove, the motion block 480 is in sliding connection with the torque adjuster 460 through the matching portion 482, the limiting hole is a waist-shaped hole 482, the matching portion is a waist-shaped structure matched with the waist-shaped hole, according to actual requirements, the matching hole may be other non-circular holes, the balls 442 are multiple balls 442, and when the motion block 480 is butted with the upper end surface of the connection rod 440, the balls can transmit the torque 442 in the -th groove and the second groove, and when the motion block is separated from the connection rod.
The torque adjusting piece 460 is in threaded connection with the connecting piece 430, an extending part 462 is arranged at the upper end of the torque adjusting piece 460, and an adjusting hole 464 is arranged on the extending part 462. The extension of the adjustment tool into the adjustment hole 464 causes the torque adjustment member 460 to rotationally move relative to the connection member 430, thereby adjusting the size of the disc spring 470 and controlling the amount of torque applied during the screwing process.
The screw capping mechanism 40 further comprises a linear bearing 444, the housing 420 comprises an upper housing 420 and a lower housing 420 which are detachably connected, the linear bearing 444 is sleeved outside the connecting rod 440, the linear bearing 444 is connected with the lower housing 420, a step position 434 is arranged in the connecting piece 430, the end of the connecting rod 440 close to the ball 442 is limited on the step position 434, the housing 420 is arranged into the upper housing 420 and the lower housing 420 which are detachably connected, the assembly and the disassembly are convenient, the linear bearing 444 is arranged to enable the connecting rod 440 to rotate more smoothly, and the step position 434 is arranged in the connecting piece 430, so that the connecting rod 440 is limited in the connecting piece 430.
The capping mechanism 40 further includes a connecting shaft 412, a fixing frame 110 connected to the transmission belt is disposed at the th side of the cantilever 10, an end of the connecting shaft 412 is connected to an output shaft of the third driving motor 410, an end of the connecting shaft is connected to the housing 420, the third driving motor 410 is mounted on the fixing frame 110, the connecting shaft 412 is rotatably connected to the fixing frame 110, the torque of the third driving motor 410 is transmitted to the housing 420 through the connecting shaft 412, the connecting shaft 412 is rotatably connected to the fixing frame 110, the fixing frame 110 does not interfere with the rotation of the fixing frame 110, and the capping mechanism 40 is connected to the cantilever 10 through the fixing frame 110.
Referring to FIGS. 1-4, methods of cell centrifugation include the steps of:
a. the centrifugal turntable 20 and the sample turntable positioned at the periphery thereof rotate to the initial position, so that the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins 210 in the circumferential direction of the centrifugal turntable 20;
b. the cantilever 10 drives the screw-capping mechanism 40 and the sampling mechanism 50 thereon to move to the upper part of the sample turntable, the cantilever 10 moves after the screw-capping mechanism 40 descends to unscrew the cap of the sample bottle at the corresponding position, the sampling mechanism 50 descends to suck the sample in the uncapped sample bottle, the cantilever 10 moves again, the sampling mechanism 50 moves to the upper part of the centrifugal bin 210 corresponding to the centrifugal turntable 20 and the current sample bottle, and the sampling mechanism 50 descends to drip the sample into the centrifugal tube 212 in the centrifugal bin 210; the cantilever 10 moves again, and the screw-on mechanism 40 descends to screw on the uncapped sample bottle cap;
c. the centrifugal turntable 20 and the sample turntable respectively rotate by a specific angle, so that the positions of the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins 210 in the circumferential direction of the centrifugal turntable 20, and the sample bottles to be processed and the centrifugal tubes 212 in the centrifugal bins 210 are positioned in the moving stroke range of the cantilever 10;
d. repeating the step b and the step c until the sample on the sample turntable is transferred;
e. and starting the centrifugal turntable to perform centrifugal treatment.
The cytocentrifuge method can be carried out by adopting the above cytospin, the screw cap mechanism 40 and the sampling mechanism 50 are driven to move left and right by the cantilever 10, samples are taken by screwing the screw cap , the efficiency is high, the samples cannot be polluted, meanwhile, the sample turntable and the centrifugal turntable 20 rotate, unprocessed sample bottles and the centrifugal bin 210 are moved to the corresponding position of the cantilever 10, the pipetting efficiency is improved by , the automatic centrifugal operation of the cells is realized, when the sample bottles on the sample turntable are in an open state, the steps of opening the cap and locking the cap are not needed, wherein the step of unscrewing the cap and screwing the cap by the screw cap mechanism 40 in the step b can be not implemented, and the sampling mechanism directly carries out the sampling operation.
Wherein, step e specifically comprises the following steps:
e1, stopping after the centrifugal turntable 20 is centrifuged for a preset time, moving the cantilever 10 to the upper part of the centrifugal turntable, and descending the sampling mechanism 50 to suck the supernatant in the centrifugal tube 212;
e2, starting the centrifugal turntable 20 again for centrifugation;
e3, repeating the steps e1 and e2 until the expected centrifugal effect is achieved.
Imbibition operation is carried out automatically through sampling mechanism 50, saves loaded down with trivial details manual operation, and centrifugal efficiency is higher.
In the step c, multiple rows of centrifuge tubes 212 for placing samples are arranged in each centrifuge bin 210, when rows of centrifuge tubes 212 have samples, the sample turntable rotates by a preset angle, so that other to-be-processed sample bottles in the circumferential direction of the sample turntable correspond to the current centrifuge bin 210, and the other to-be-processed sample bottles and the centrifuge tubes in the current centrifuge bin are located in the cantilever movement stroke range, the centrifuge turntable 20 does not rotate, the sampling mechanism 50 on the cantilever 10 sucks the samples in the sample bottles at the corresponding positions, the cantilever 10 moves to drop the samples into another rows of centrifuge tubes 212 in the centrifuge bin 210, and the centrifuge turntable 20 is rotated until all the centrifuge tubes 212 in the current centrifuge bin 210 have samples, so that another centrifuge bin 210 rotates into the cantilever 10 movement stroke range.
In the steps a and c, when the centrifugal turntable 20 is aligned with the sample turntable, the centrifugal turntable 20 is driven to rotate by adopting an th positioning motor, the th positioning motor is connected with a transmission shaft of the centrifugal turntable 20 through an electromagnetic clutch 620, in the step e, the centrifugal turntable 20 is driven by adopting a centrifugal motor 610 during centrifugal processing, at the moment, the electromagnetic clutch 620 is disconnected with the transmission shaft of the centrifugal turntable 20, the centrifugal turntable 20 is driven to stop after being driven for a preset time through the centrifugal motor 610, the electromagnetic clutch 620 is connected with the transmission shaft of the centrifugal turntable 20 again, the th positioning motor drives the centrifugal turntable 20 to rotate so as to be aligned with the sampling mechanism 50, after the sampling mechanism 50 sucks the supernatant in the centrifugal tube 212, the electromagnetic clutch 620 is disconnected with the transmission shaft of the centrifugal turntable 20 again, the centrifugal motor 610 drives the centrifugal turntable 20 again to carry out centrifugal processing, the centrifugal motor 610 is adopted to realize high-speed centrifugal operation of the centrifugal turntable 20, and the th positioning motor is selectively connected with the centrifugal turntable 20, so that the alignment operation of the centrifugal turntable 20 is realized.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (17)
1, kind of cytospin, characterized in that, including the cantilever, be used for placing the centrifugal carousel in centrifugal storehouse and be used for placing the sample carousel of sample bottle, the sample carousel is located centrifugal carousel periphery, but the cantilever is located centrifugal carousel and sample carousel top and horizontal migration, be equipped with sampling mechanism and screw cap mechanism on the cantilever, just screw cap mechanism, sampling mechanism can reciprocate relatively the cantilever, screw cap mechanism sets up in the th side of cantilever, sampling mechanism sets up in the second side of cantilever, centrifugal carousel passes through the drive of actuating mechanism, the sample carousel passes through the drive of second actuating mechanism, cytospin still includes control mechanism, be equipped with the positioning switch on the centrifugal carousel, be equipped with the second positioning switch on the sample carousel, the th positioning motor of actuating mechanism, the second positioning motor of second actuating mechanism positioning switch and second positioning switch respectively with control mechanism electric connection.
2. The cytospin of claim 1, wherein the driving mechanism further comprises a centrifugal motor and an electromagnetic clutch, the centrifugal motor is connected with the transmission shaft of the centrifugal turntable, and the positioning motor is connected with the transmission shaft of the centrifugal turntable through the electromagnetic clutch.
3. The cytospin centrifuge of claim 2, wherein the second driving mechanism further comprises a synchronous belt and a gear, the gear is connected with the output shaft of the second positioning motor, the synchronous belt is sleeved outside the sample turntable, and the outer wall of the synchronous belt is provided with teeth engaged with the gear.
4. A cytospin according to , wherein the sampling mechanism comprises a tube rack and a sampling tube arranged on the tube rack, the cantilever is provided with a th driving motor and a longitudinally arranged transmission belt, the end of the transmission belt is rotationally connected with the rotating shaft of the th driving motor, the other end of the transmission belt is wound on a pulley, the tube rack is connected with the side of the transmission belt, the sampling mechanism further comprises a pipette, the pipette is arranged on the tube rack, and the pipette and the sampling tube are arranged at intervals in the front-back direction along the moving direction of the cantilever.
5. The cytospin centrifuge of claim 4, wherein the cantilever is provided with a through hole at a position opposite to the belt, the screw-cap mechanism is connected to the belt through the through hole, and the side of the screw-cap mechanism connected to the belt is opposite to the side of the sampling mechanism connected to the belt, so that the screw-cap mechanism and the sampling mechanism move in different directions along with the movement of the belt.
6. The cell centrifuge of claim 4, wherein the sample disk is provided with a plurality of sample positions for placing sample bottles, a plurality of sampling positions for placing sampling tubes, and a plurality of avoiding positions for placing pipettes, around the circumferential direction of the sample disk, and the sample positions, the sampling positions, and the avoiding positions are located in the circumferential direction of the sample disk at different radii.
7. The cytospin centrifuge of any one of , wherein the centrifuge disk has a plurality of detents around its circumference, the centrifugation container is detachably connected to the detents, and the centrifugation container can rotate around the detents.
8. The cytocentrifuge of claim 4, further comprising a worktable, wherein the sample turntable and the centrifuge turntable are rotatably disposed on the worktable, the worktable is provided with a support frame, the support frame is provided with a beam arranged along the radial direction of the sample turntable or the centrifuge turntable, the cantilever is slidably disposed on the beam by the control of the second driving motor, and the beam is provided with two cantilevers.
9. The cytospin centrifuge of claim 8, wherein each cantilever arm has two of the screw-capping mechanism, two of the sampling tubes and two of the pipette tubes, the two screw-capping mechanisms are juxtaposed, the two of the sampling tubes are juxtaposed, the two of the pipette tubes are juxtaposed, and the distance between the juxtaposed two screw-capping mechanisms, the two of the sampling tubes and the two of the pipette tubes is equal to the distance between adjacent sample bottles on the sample carousel.
10. The cytospin machine of claim 2 or 3, further comprising a scanner located above the sample carousel and the centrifugation carousel, the scanner being configured to scan barcodes on centrifugation wells and/or barcodes on sample vials and/or barcodes on centrifuge tubes in the centrifugation wells, the scanner being electrically connected to the control mechanism.
11. The cytospin according to claim 2 or 3, further comprising a protector for detecting the rotation speed of the centrifugal motor, wherein a protective cover is provided outside the centrifugal turntable, and when the protector detects that the centrifugal motor is operating, the protective cover cannot be opened and the electromagnetic clutch cannot be operated.
12. The cytospin according to claim 2 or 3, characterized in that, it further comprises a balance detector for detecting whether the left and right of the sample to be centrifuged on the centrifugal turntable are balanced, when the left and right of the sample to be centrifuged on the centrifugal turntable are not balanced, the balance detector sends out a warning prompt.
13. The cell centrifuge of claim 5, wherein a fixing frame connected to the transmission belt is disposed on a th side of the cantilever, the screw-capping mechanism includes a third driving motor, a connecting shaft, a housing, a connecting member, a connecting rod, a torque adjusting member, a disc spring, a motion block, a one-way bearing, and a cover body matched to the cap of the sample bottle, an end of the connecting shaft is connected to an output shaft of the third driving motor, an end of the other is connected to the housing, the third driving motor is mounted on the fixing frame, the connecting shaft is rotatably connected to the fixing frame, the housing is provided with a sliding slot along a longitudinal direction, the connecting member is mounted in the housing, the sliding slot is slidably engaged with the sliding slot, the housing is provided with an elastic member abutting against the upper portion of the connecting member, the one-way bearing is disposed in the connecting member, the one-way bearing is disposed outside the connecting rod, an outer ring of the one-way bearing is connected to the connecting member, the torque adjusting member is threadedly engaged with the connecting member, an upper end of the torque adjusting member is provided with an extending slot, the extending portion is provided with an adjusting hole, the disc spring and the motion block are disposed in the torque adjusting slot, a plurality of the sample bottle-adjusting slot is provided on an upper end of the connecting rod, a plurality of the sample bottle-accommodating slot is provided on an inner wall of the upper end of the sample bottle, a plurality of the sample bottle-matching end of the sample bottle, the sample bottle-matching end of the connecting rod, the sample bottle-matching.
14, method for cell centrifugation, comprising the steps of:
a. the centrifugal turntable and the sample turntable positioned on the periphery of the centrifugal turntable rotate to initial positions, so that the positions of the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins in the circumferential direction of the centrifugal turntable;
when the sample bottles on the sample turntable are tightly covered by the covers, before the sampling mechanism samples, the cantilever drives the cover screwing mechanism on the sample turntable to move to the upper part of the sample turntable, and after the cover screwing mechanism descends and unscrews the covers of the sample bottles at the corresponding positions, the step b is carried out; after the step b is finished, the cantilever is moved to the position above the sample turntable again, the cover screwing mechanism descends to screw down the uncapped sample bottle cover, and then the step c is carried out;
b. the cantilever drives the sampling mechanism on the cantilever to move to the upper part of the sample turntable, the sampling mechanism descends to absorb the sample in the sample bottle, the cantilever moves again, the sampling mechanism moves to the upper part of the centrifugal turntable, which corresponds to the current sample bottle, of the centrifugal bin, and the sampling mechanism descends to drop the sample into the centrifugal tube in the centrifugal bin;
c. the centrifugal turntable and the sample turntable respectively rotate for a specific angle, so that the positions of the sample bottles in the circumferential direction of the sample turntable correspond to the positions of the centrifugal bins in the circumferential direction of the centrifugal turntable, and the sample bottles to be processed and the centrifugal tubes in the centrifugal bins are positioned in the range of the cantilever movement stroke;
d. repeating the step b and the step c until the sample on the sample turntable is transferred;
e. and starting the centrifugal turntable to perform centrifugal treatment.
15. A method of centrifuging cells according to claim 14 wherein step e comprises the steps of:
e1, stopping after centrifuging the centrifugal turntable for a preset time, moving the cantilever above the centrifugal turntable, and descending the sampling mechanism to suck the supernatant in the centrifugal tube;
e2, restarting the centrifugal turntable for centrifugation;
e3, repeating the steps e1 and e2 until the expected centrifugal effect is achieved.
16. The method of centrifuging cells of claim 15,
in the step c, multiple rows of centrifuge tubes for placing samples are arranged in each centrifuge bin, after rows of centrifuge tubes are dripped with samples, the sample turntable rotates by a preset angle, so that other to-be-processed sample bottles in the circumferential direction of the sample turntable correspond to the current position of the centrifuge bin, and the other to-be-processed sample bottles and the centrifuge tubes in the current centrifuge bin are located in the cantilever moving stroke range, the centrifuge turntable does not rotate, a sampling mechanism on the cantilever sucks the samples in the sample bottles in the corresponding positions, the cantilever moves to drip the samples into rows of centrifuge tubes in the centrifuge bin, the centrifuge turntable rotates until the centrifuge tubes in the current centrifuge bin drip the samples, and another centrifuge bin rotates into the cantilever moving stroke range.
17. The method of centrifuging cells of claim 16,
when the centrifugal turntable is aligned with the sample turntable, an th positioning motor is adopted to drive the centrifugal turntable to rotate, and the th positioning motor is connected with a transmission shaft of the centrifugal turntable through an electromagnetic clutch;
the centrifugal turntable is driven by a centrifugal motor during centrifugal treatment, the electromagnetic clutch is disconnected with a transmission shaft of the centrifugal turntable at the moment, the centrifugal turntable is stopped after being driven by the centrifugal motor for a preset time, the electromagnetic clutch is connected with the transmission shaft of the centrifugal turntable again, an th positioning motor drives the centrifugal turntable to rotate so as to align the centrifugal turntable with a sampling mechanism, and after the sampling mechanism sucks supernatant in a centrifugal tube, the electromagnetic clutch is disconnected with the transmission shaft of the centrifugal turntable again, and the centrifugal motor drives the centrifugal turntable again to perform centrifugal treatment.
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| CN106680065B (en) * | 2017-01-20 | 2018-06-29 | 济南英盛生物技术有限公司 | A kind of cell block embedding machine and embedding method |
| CN106989950A (en) * | 2017-04-26 | 2017-07-28 | 江西欧范医疗器械有限公司 | Clutch apparatus for mixing mechanism of sampling |
| CN108802413A (en) * | 2018-09-19 | 2018-11-13 | 基蛋生物科技股份有限公司 | Quantitative in vitro detecting instrument |
| US20210008541A1 (en) * | 2019-06-20 | 2021-01-14 | Accelerate Diagnostics, Inc. | Instrument and methods for automated sample preparation for microorganism identification and differentiation |
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| CN111781390B (en) * | 2020-07-27 | 2023-09-05 | 苏州奥美泰克生物技术有限公司 | Four-channel double-circulation synchronous sample transferring system and method |
| CN112014571A (en) * | 2020-08-20 | 2020-12-01 | 金华科生物技术河北有限公司 | Semi-quantitative detection device for C-reactive protein colloidal gold method |
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