CN206038392U - Cell centrifuge - Google Patents

Abstract

The utility model relates to a sample carousel that cell centrifuge, cell centrifuge include the cantilever, are used for to place the centrifugal carousel in centrifugal storehouse and be used for placing the sample bottle, sample carousel are located centrifugal carousel periphery, and the cantilever is located centrifugal carousel and sample carousel top and adjustable horizontally, be equipped with sampler on the cantilever and construct, just sampler constructs relatively, and the cantilever reciprocates. Sampler construct to draw the sample in this sample bottle, and horizontal migration drips the sample to the centrifugal storehouse in to centrifugal carousel top again, accomplishes the transfer operation of a sample, moves liquid and accomplishes the back, and the sample of centrifugal carousel in to centrifugal storehouse carries out the centrifugation, realizes cell automation centrifugal process.

Description

Cytospin

Technical field

This utility model is related to medical instruments field, more particularly to a kind of cytospin.

Background technology

By observing the sectioning cells of each tissue of human body, it is known that whether human body has pathological changes.Sectioning cells process：First Sampling, reuse centrifuge carries out the centrifugally operated of special time, number of times to sample；Discard containing mucus, bib and The supernatant of other non-viable non-apoptotic cell compositions；Add cell adhesion parcel liquid；The thin-layer cell of cell monolayer distribution is made finally Smear, then go artificially colored.But the manual operation being centrifuged to cell is typically necessary, is discarded containing mucus, red The supernatant of cell debriss and other non-viable non-apoptotic cell compositions, these early stages process often all hand-manipulated carrying out；Artificial mistake Turn pale and easily cause diagnosis cell loss, and operator's directly contact specimen, security protection are poor.

The content of the invention

Based on this, it is necessary to for the defect of prior art, there is provided a kind of cytospin, cell automatic centrifugation can be realized Process, it is to avoid human contact's sample causes cell loss or dirty.

Its technical scheme is as follows：

A kind of cytospin, including cantilever, the centrifugal turntable for placing centrifugation storehouse and the sample for placing sample bottle Product rotating disk, the sample rotating disk are located at centrifugal turntable periphery, and the cantilever is located above centrifugal turntable and sample rotating disk and can water Translation is dynamic, and the cantilever is provided with sampling mechanism, and the sampling mechanism energy opposing cantilevers are moved up and down, on the sample rotating disk Multiple sample positions for placing sample bottle are provided with around its circumferential direction.

Its further technical scheme is as follows：

The centrifugal turntable by the second drive mechanism drive, second drive mechanism include be centrifuged motor, electromagnetism from Clutch and the second positioning motor, the centrifugation motor are connected with the power transmission shaft of the centrifugal turntable, and second positioning motor leads to The power transmission shaft that electromagnetic clutch is crossed with the centrifugal turntable is connected.

The sample rotating disk is driven by the first drive mechanism, and first drive mechanism includes the first positioning motor, same Step band and gear, the gear are connected with the output shaft of first positioning motor, and the Timing Belt is set in the sample and turns Outside disk, and the Timing Belt outer wall is provided with the tooth engaged with the gear.

Described cytospin also includes controlling organization, and the centrifugal turntable is provided with the first position-sensing switch, the sample Product rotating disk is provided with the second position-sensing switch, second positioning motor, the first positioning motor, the first position-sensing switch and the second positioning Switch is electrically connected with the controlling organization respectively.

The sampling mechanism includes pipe support and the probe tube being arranged on the pipe support, and the cantilever is provided with the first driving Motor and the transmission belt being longitudinally arranged, one end of the transmission belt are rotated with the rotary shaft of first motor and are connected, and are passed The other end of dynamic band is wound on belt wheel, and the pipe support is connected with the wherein side of the transmission belt.

The sampling mechanism also include pipette, the pipette be arranged on the pipe support on, on the sample rotating disk around Its circumferential direction is additionally provided with multiple sampling positions and multiple avoidance positions for avoiding pipette for placing probe tube, and described Sample position, sampling position and avoidance position are located on the circumferencial direction of sample rotating disk different radii, around its circumference on the centrifugal turntable Direction is provided with multiple screens, and the centrifugation storehouse is detachably connected with the screens, and the centrifugation storehouse can be rotated around the screens.

Described cytospin also includes screw capping mechanism, and the screw capping mechanism is arranged on the first side of cantilever, described to take Model machine structure is arranged on the second side of cantilever, offers long through-hole, the lid tightener at the cantilever position relative with transmission belt Structure is connected with the transmission belt by the long through-hole, and the side that is connected with transmission belt of screw capping mechanism with the sampling mechanism with The side of transmission belt connection is oppositely arranged, and screw capping mechanism is moved towards different directions with the mobile of transmission belt respectively from sampling mechanism It is dynamic.

Described cytospin also includes workbench, and the sample rotating disk, centrifugal turntable are rotatably arranged in work On platform, the workbench is provided with bracing frame, and support frame as described above is provided with to be arranged along sample rotating disk or centrifugal turntable radial direction Crossbeam, the cantilever by the second motor control be slidably disposed on crossbeam, the crossbeam is provided with two institutes Cantilever is stated, each cantilever is provided with two screw capping mechanisms, two probe tubes and two pipette, two screw cappings Mechanism is set up in parallel, and two probe tubes are set up in parallel, and two pipette are set up in parallel, and two lid tighteners The spacing phase of the spacing that structure, two probe tubes and two pipette are set up in parallel and neighboring samples bottle on sample rotating disk Deng.

Described cytospin also includes the scanning device above sample rotating disk with centrifugal turntable, and the scanning device is used Bar code in bar code and/or centrifugation storehouse in the bar code on scanning centrifugation storehouse and/or on sample bottle on centrifuge tube, the scanning device It is electrically connected with the controlling organization；Also include the protector for detecting centrifugation motor speed, the centrifugal turntable is externally provided with Protective cover, when the protector detects the centrifugation motor just in the running, the protective cover cannot be opened and the electromagnetism Clutch cannot run；Also include for detecting balanced detector that whether sample to be centrifuged on centrifugal turntable or so balances, When detecting on centrifugal turntable when sample being centrifuged or so is uneven, balanced detector sends warning and reminds.

First side of the cantilever is provided with the fixed mount being connected with the transmission belt, and the screw capping mechanism includes the 3rd driving Motor, connecting shaft, housing, connector, connecting rod, moment of torsion regulating part, disc spring, moving mass, unilateral bearing and with sample bottle cover The cover body of matching, described connecting shaft one end are connected with the output shaft of the 3rd motor, and the other end is connected with the housing, 3rd motor is arranged on the fixed mount, and the connecting shaft is rotated with the fixed mount and is connected, on the housing The chute along longitudinal direction is provided with, the connector is provided with extension bar, connector is arranged in housing, the extension bar and the cunning Groove is slidably matched, and is provided with the elastic component being connected to above connector in the housing, and the unilateral bearing is arranged on the connection In part, and unilateral bearing is set in outside the connecting rod, and the outer ring of unilateral bearing is connected with the connector, and the moment of torsion is adjusted Part is threadedly coupled with the connector, and the upper end of the moment of torsion regulating part is provided with extension, offers regulation on the extension Hole, the disc spring and moving mass are arranged in moment of torsion regulating part, and one end of disc spring is connected on the inwall of moment of torsion regulating part, separately One end is connected to the upper end of moving mass, and the lower end of the moving mass passes through ball engagement with the upper end of connecting rod, under connecting rod End is stretched out the housing and is connected with the upper surface of the cover body, offers and the sample bottle cover on the lower surface of the cover body The holding tank of matching, the circumferentially-spaced of the sample bottle cover are provided with multiple chuck, offer many on the side wall of the holding tank The individual draw-in groove matched with the chuck.

Below the advantage or principle of preceding solution are illustrated：

Sample rotating disk is arranged on centrifugal turntable periphery by above-mentioned cytospin, and cantilever level movement, sampling mechanism are drawn Sample in the sample bottle, then be moved horizontally to above centrifugal turntable by sample drip to centrifugation storehouse in, complete a sample turn Move operation.The pipetting of a sample is completed often, the sample rotating disk and/or centrifugal turntable are rotated, by pending sample Bottle is turned in the range of the shift motion of cantilever with centrifugation storehouse, and cantilever only need to be moved in one direction, and liquid relief speed is fast, and every time Pipetting, sampling mechanism are only sampled operation to current location sample bottle, will not pollute other samples.After the completion of liquid relief, Centrifugal turntable is centrifuged to the sample being centrifuged in storehouse, in hgher efficiency without the need for manual operations, is realized that cell automatic centrifugation is processed, is kept away Manpower-free's contact sample causes cell loss or dirty.

Description of the drawings

Schematic diagrams one of the Fig. 1 for cytospin described in this utility model embodiment；

Schematic diagrams two of the Fig. 2 for cytospin described in this utility model embodiment；

Fig. 3 is the partial schematic diagram after cytospin hidden parts part described in this utility model embodiment；

Schematic diagrams three of the Fig. 4 for cytospin described in this utility model embodiment；

Generalized sections of the Fig. 5 for screw capping mechanism described in this utility model embodiment；

Close-up schematic views of the Fig. 6 for Fig. 5；

Fig. 7 is the schematic diagram after screw capping mechanism hidden parts part described in this utility model embodiment；

Fig. 8 is the schematic diagram after screw capping mechanism hidden parts part described in this utility model embodiment.

Description of reference numerals：

10th, cantilever, 110, fixed mount, the 120, first motor, 130, transmission belt, 140, belt wheel, 150, long through-hole, 20th, centrifugal turntable, 210, centrifugation storehouse, 212, centrifuge tube, 220, screens, 30, sample rotating disk, 310, sample bottle, 312, chuck, 320th, sample position, 330, sampling position, 340, avoid position, 40, screw capping mechanism, the 410, the 3rd motor, 412, connecting shaft, 420, Housing, 422, chute, 424, elastic component, 430, connector, 432, extension bar, 434, step site, 440, connecting rod, 442, rolling Pearl, 444, linear bearing, 450, cover body, 452, holding tank, 454, draw-in groove, 460, moment of torsion regulating part, 462, extension, 464, adjust Knothole, 470, disc spring, 480, moving mass, 482, auxiliary section, 484, driving section, 490, unilateral bearing, 50, sampling mechanism, 510, Pipe support, 520, probe tube, 530, pipette, the 60, second drive mechanism, 610, centrifugation motor, 620, electromagnetic clutch, 630, the Two positioning motors, the 70, first drive mechanism, the 710, first positioning motor, 720, Timing Belt, 730, gear, 80, workbench, 810th, bracing frame, 820, crossbeam, the 830, second motor.

Specific embodiment

As shown in Figures 1 to 4, a kind of cytospin, including cantilever 10, the centrifugal turntable 20 for placing centrifugation storehouse 210 And for placing the sample rotating disk 30 of sample bottle 310, the sample rotating disk is located at 20 periphery of centrifugal turntable, and the cantilever 10 is located at Above centrifugal turntable 20 and sample rotating disk and horizontally moveable, the cantilever 10 is provided with screw capping mechanism 40 and sampling mechanism 50, And the screw capping mechanism 40,50 energy opposing cantilevers 10 of sampling mechanism move up and down.

Sample rotating disk is arranged on into 20 periphery of centrifugal turntable, cantilever 10 is moved horizontally, screw capping mechanism 40 is by sample rotating disk Sample bottle cover turn on, sampling mechanism 50 draws the sample in the sample bottle, then be moved horizontally to above centrifugal turntable 20 will Sample drip to centrifugation storehouse 210 in, cantilever 10 move again to uncap after sample bottle above, lid is tightened by screw capping mechanism 40 again, Complete the transfer operation of a sample.20 turns of the pipetting of a sample, the sample rotating disk and/or centrifugal turntable are completed often It is dynamic, pending sample bottle is turned in the range of the shift motion of cantilever 10 with centrifugation storehouse 210, cantilever 10 only need to be along a side To movement, liquid relief speed is fast, and pipetting every time, and screw capping mechanism 40 and sampling mechanism 50 are only carried out to current location sample bottle Screw capping and sampling operation, will not pollute other samples.After the completion of liquid relief, the sample in 20 pairs of centrifugation storehouses 210 of centrifugal turntable is carried out Centrifugation, often centrifugation a period of time, 10 mobile sampler structure of cantilever 50 draws the supernatant in centrifugation storehouse 210, without the need for manual operations effect Rate is higher, realizes that cell automatic centrifugation is processed, it is to avoid human contact's sample causes cell loss or dirty.According to actual need Ask, when the sample bottle on sample rotating disk is not sample bottle with a lid, the screw capping mechanism on the cantilever 10, can be not provided with, Sampling mechanism 50 is directly sampled in the sample bottle for opening wide, sampling terminate after also without carrying out screw capping operation.

As shown in figure 4, the centrifugal turntable 20 by the second drive mechanism drive 60, second drive mechanism include from Electrocardiograph 610, electromagnetic clutch 620 and the second positioning motor 630, the biography that motor 610 and the centrifugal turntable 20 is centrifuged Moving axis connects, and second positioning motor 630 is connected with the power transmission shaft of the centrifugal turntable 20 by electromagnetic clutch 620, institute It is motor to state the second positioning motor 630, and the centrifugation motor 610 is brushless electric machine.Due to the rotating speed needed for centrifugal process Greatly, and centrifugal turntable 20 needs to be accurately positioned in pipetting processes, a motor cannot meet both demands simultaneously, by arrange Second drive mechanism, when centrifugation, centrifugation motor 610 works, and centrifugal turntable 20 runs up, electromagnetic clutch 620 with The power transmission shaft of the centrifugal turntable 20 disconnects, and when positioning action is needed, centrifugation motor 610 does not work, electromagnetic clutch 620 with The power transmission shaft connection of the centrifugal turntable 20, the second positioning motor 630 drive centrifugal turntable 20 to operate, realize being accurately positioned.

As shown in figure 3, the sample rotating disk is driven by the first drive mechanism 70, first drive mechanism includes first Positioning motor 710, Timing Belt 720 and gear 730, the gear 730 are connected with the output shaft of first positioning motor 710, The Timing Belt 720 is set in outside the sample rotating disk, and 720 outer wall of the Timing Belt is provided with and is engaged with the gear 730 Tooth.Engaged with gear 730 by arranging toothed Timing Belt 720,710 drive gear 730 of the first positioning motor operates, driven Timing Belt 720 and sample rotating disk are rotated, and can arrange bearing or strainer realizes that Timing Belt 720 is elastic on Timing Belt 720 Adjust.

Described cytospin also includes controlling organization, and the centrifugal turntable is provided with the first position-sensing switch, the sample Product rotating disk is provided with the second position-sensing switch, second positioning motor, the first positioning motor, the first position-sensing switch and the second positioning Switch is electrically connected with the controlling organization respectively.Controlling organization controls the operating of the second positioning motor, drives centrifugal turntable to rotate, Controlling organization controls the operating of the first positioning motor, drives sample rotating disk to rotate, and the centrifuge tube and sample in storehouse is centrifuged on centrifugal turntable Sample bottle on rotating disk is one-to-one, in pipetting processes, drives centrifugal turntable to rotate certain angle by the second positioning motor Spending the first positioning motor drives sample rotating disk to turn an angle, and the centrifuge tube on centrifugal turntable after the anglec of rotation is turned with sample Sample bottle on disk not only wants position correspondence, but also must be corresponding with the sampling mechanism position on cantilever, it is ensured that liquid relief is errorless, leads to Cross the first position-sensing switch and the second position-sensing switch and be capable of achieving being accurately positioned for centrifugal turntable and sample disk position, further ensure that The accuracy of liquid relief.First position-sensing switch, the second position-sensing switch described in the present embodiment are photoswitch, arrange one in initial position Matte or a gap, when blocking or when printing opacity, it is assumed that being initial position, then calculate the anglec of rotation to position.

As Figure 1-5, the screw capping mechanism 40 is arranged on the first side of cantilever 10, and the sampling mechanism 50 is arranged on outstanding Second side of arm 10, the sampling mechanism 50 include pipe support 510, probe tube 520 and pipette 530, the probe tube 520 and suction Liquid pipe 530 is arranged on the pipe support 510, and pipette 530 is set along 10 moving direction time interval of cantilever with probe tube 520 Put.First side of the cantilever 10 is fixed with the first motor 120, and the second side of the cantilever 10 is provided with the biography being longitudinally arranged Dynamic band 130, one end of the transmission belt 130 are rotated with the rotary shaft of first motor 120 and are connected, transmission belt 130 The other end is wound on belt wheel 140, and the position relative with transmission belt 130 of the cantilever 10 offers long through-hole 150, the lid tightener Structure 40 is connected with the side of transmission belt 130 by the long through-hole 150, and the sampling mechanism 50 is another with the transmission belt 130 Side connects, and the side that is connected with transmission belt 130 of screw capping mechanism 40 be connected with transmission belt 130 with the sampling mechanism 50 one Side is oppositely arranged, and screw capping mechanism 40 is moved towards different directions with the mobile of transmission belt 130 respectively from sampling mechanism 50.It is described First motor 120 starts, and drives transmission belt 130 to rotate, due to screw capping mechanism 40 and sampling mechanism 50 respectively with transmission belt 130 relative both sides connections, when the transmission belt 130 is rotated, screw capping mechanism 40 rises, and sampling mechanism 50 just declines therewith, twists Lid mechanism 40 declines, and sampling mechanism 50 just rises therewith, makes screw capping mechanism 40 non-interference when in use with sampling mechanism 50, and Only screw capping mechanism 40 is driven to move up and down with sampling mechanism 50 simultaneously by a motor, simple structure is cost-effective.It is described to take Sample pipe 520 is used for the sample of pipette samples bottle and drops in centrifuge tube, and the pipette 530 is for producing after centrifuge tube is centrifuged Supernatant is siphoned away, and pipette 530 is arranged along 10 moving direction time interval of cantilever with probe tube 520, so moves forward and backward cantilever The para-position of probe tube 520, pipette 530 and centrifuge tube is capable of achieving, it is easy to operate.

On the sample rotating disk around its circumferential direction be provided with multiple sample positions 320 for placing sample bottle, it is multiple for Place the sampling position 330 and multiple avoidance positions 340 for avoiding pipette 530 of probe tube 520, and the sample position 320, take Sample position 330 and avoidance position 340 are located on the circumferencial direction of sample rotating disk different radii.So arrange, when sample rotating disk is turned to It is during with 10 correspondence position of cantilever, movable by cantilever 10, can be easy to carry out sample bottle screw capping operation, to probe tube 520 Replacement operation is carried out, when the avoidance position 340 is used for probe tube sampling, the pipette 530 for declining together is avoided, it is to avoid inhale Liquid pipe 530 is interfered with sample rotating disk.The cantilever is provided with the orifice ring for taking off probe tube 520, and the internal diameter of the orifice ring is little During moving on the external diameter of the probe tube 520, probe tube 520, the sampling head for being socketed probe tube 520 is passed through from orifice ring, And probe tube 520 cannot be quick so as to realize from orifice ring through being forced down under sampling head more than orifice ring internal diameter due to external diameter The purpose of de- probe tube 520.

Multiple screens 220, the centrifugation storehouse 210 and the screens are provided with around its circumferential direction on the centrifugal turntable 20 220 are detachably connected, and the centrifugation storehouse 210 can be rotated around the screens 220.By screens 220 make centrifugation storehouse 210 can from On heart rotating disk 20, centrifugation storehouse 210 directly can be removed after being centrifuged and be moved on stained preparation machine by sample, without the need for single sample point Do not shift, simple and efficient to handle, centrifugation storehouse 210 can be rotated around screens 220, and the change of 210 position of storehouse is centrifuged in being easy to centrifugal process Change.

Described cytospin also includes workbench 80, and the sample rotating disk, centrifugal turntable 20 are rotatably arranged in On workbench 80, the workbench 80 is provided with bracing frame 810, and support frame as described above 810 is provided with and turns along sample rotating disk or centrifugation The crossbeam 820 of 20 radial direction of disk arrangement, the cantilever 10 are slidably disposed on crossbeam by the control of the second motor 830 On 820, the crossbeam 820 is provided with two cantilevers 10.Two cantilevers 10 are set and can improve liquid relief and imbibition efficiency, cantilever 10 move along the radial direction of sample rotating disk or centrifugal turntable 20, so that after sample rotating disk or centrifugal turntable 20 rotate thereon Sample bottle, the position in centrifugation storehouse 210 can be in the shift motion of cantilever 10, and para-position is more accurate.

Each cantilever 10 is provided with two screw capping mechanisms, 40, two probe tubes 520 and two pipette 530, Liang Ge screw capping mechanisms 40 are set up in parallel, and two probe tubes 520 are set up in parallel, and two pipette 530 are set side by side Put, and the spacing that is set up in parallel of two screw capping mechanisms, 40, two probe tubes 520 and two pipette 530 with On sample rotating disk, the spacing of neighboring samples bottle is equal.So arrange, when operating every time, screw capping mechanism 40 can be simultaneously to two samples Bottle carries out screw capping, and sampling mechanism 50 can carry out taking liquid and imbibition to two parts of samples simultaneously, in hgher efficiency.

Described cytospin also includes controlling organization and the scanning device above sample rotating disk with centrifugal turntable 20, The scanning device is used for scanning centrifuge tube in bar code and/or the bar code on sample bottle and/or the centrifugation storehouse 210 on centrifugation storehouse 210 Bar code on 212, the scanning device are electrically connected with the controlling organization, the controlling organization and the screw capping mechanism 40, are taken Model machine structure 50 is electrically connected with.Controlling organization is fed back to by scanning device scanning correspondence bar code, so that sample bottle and centrifugation storehouse 210 Centrifuge tube 212 more accurately can correspond to, it is to avoid there is mistake in sample.

Described cytospin also includes the protector for detecting centrifugation 610 rotating speed of motor, the centrifugal turntable 20 Be externally provided with protective cover, when the protector detect it is described centrifugation motor 610 just in the running, the protective cover cannot open and The electromagnetic clutch 620 cannot run.During centrifugation protective cover close, it is to avoid sample splash etc., cause personnel to injure, only when After protector detects the centrifugation stopping of motor 610, protective cover could be normally-open, and electromagnetic clutch 620 normally could run, will The power of the second positioning motor 630 reaches centrifugal turntable 20.

Described cytospin is also included for detecting what whether sample to be centrifuged on centrifugal turntable 20 or so balanced Balanced detector, when detecting on centrifugal turntable 20 when sample being centrifuged or so is uneven, balanced detector sends warning and carries Wake up.During 20 centrifugally operated of centrifugal turntable, rotating speed is fast, if the left and right sides is uneven, easily causes and rotates unstable, and sample flies out The problems such as, by arranging balanced detector, it is adjusted once detecting imbalance and sending warning in time, it is to avoid dangerous situation goes out It is existing.

As viewed in figures 5-8, the screw capping mechanism 40 includes the 3rd motor 410, housing 420, connector 430, connection Bar 440 and the cover body 450 matched with sample bottle cover, the housing 420 are provided with the chute 422 along longitudinal direction, the connector 430 are provided with extension bar 432, and connector 430 is arranged in housing 420, and the extension bar 432 is slided with the chute 422 and matched somebody with somebody Close, in the housing 420, be provided with the elastic component 424 being connected to above connector 430, the 3rd motor 410 with it is described Housing 420 is connected, and 440 one end of the connecting rod is stretched into and is connected with the connector 430 in housing 420, and connecting rod 440 is another One end is connected with the cover body 450.3rd motor 410 is rotated, and drives the housing 420 being attached thereto to rotate, so as to drive Connector 430 is rotated, and then drives connecting rod 440 and cover body 450 to rotate, and 450 sets of cover body to be arranged and drive lid outside sample bottle lid Son rotation, so as to turn on or tighten lid.Offer on the lower surface of cover body 450 described in the present embodiment and the sample The holding tank 452 of bottle cover matching, the upper surface of the cover body 450 are connected with the connecting rod 440, the sample bottle cover It is circumferentially-spaced to be provided with multiple chuck 312, multiple draw-in grooves matched with the chuck are offered on the side wall of the holding tank 452 454.When cover body 450 is set on lid, screens 220 is probably what is staggered with draw-in groove 454, and now lid can not be turned, with The rotation of cover body 450, connector 430 moves compression elastic piece 424 along chute 422, makes elastic component 424 produce elastic deformation, When draw-in groove 454 is with 220 para-position of screens, 424 resilience of elastic component makes the draw-in groove 454 of cover body 450 nested with the screens 220 of lid, So that the position of 40 energy self adaptation sample bottle cover of screw capping mechanism, is coordinated with draw-in groove 454 by screens 220, cover body 450 is turned on After lid, lid is still in cover body 450, after sample is completed, and cover body 450 is tightened in lid on counter sample bottle again. According to the actual requirements, cover body 450 may also be employed other connected modes with lid, such as on cover body 450 setting clamping jaw structure or Lid is with housing 420 by the way of magnetic is connected etc..

Further, during for avoiding tightening lid, torsion is excessive to cause lid damage or the 3rd motor 410 to damage Bad, the screw capping mechanism 40 also includes moment of torsion regulating part 460, disc spring 470, moving mass 480 and unilateral bearing 490, the moment of torsion Regulating part 460 is connected with the connector 430, and the unilateral bearing 490 is arranged in the connector 430, and unilateral bearing 490 are set in outside the connecting rod 440, and the outer ring of unilateral bearing 490 is connected with the connector 430, the disc spring 470 and fortune Motion block 480 is arranged in moment of torsion regulating part 460, and one end of disc spring 470 is connected on the inwall of moment of torsion regulating part 460, another End is connected to the upper end of moving mass 480, and the lower end of the moving mass 480 is coordinated by ball 442 with the upper end of connecting rod 440. The unilateral bearing 490 is fixed outer ring, and inner ring can only be rotated towards the direction for tightening lid, and when twisting the lid off, the 3rd drives electricity The unilateral bearing 490 that the torsion of machine 410 is reached housing 420, connector 430 and is connected with connector 430, now unilateral bearing 490 inner ring cannot be relatively rotated with outer ring, and torsion directly reaches connecting rod 440 to cover body 450 by unilateral bearing 490；Tighten During lid, torsion reaches moment of torsion regulating part 460 by connector 430, is then passed to disc spring 470, moving mass 480, ball 442 to even Extension bar 440 and cover body 450, if the 3rd motor 410 is continued to run with after lid is tightened, 480 opposing torque regulating part of moving mass Move on 460, disc spring 470 is deformed upon makes moving mass 480 separate the work for playing slipping protection with connecting rod 440 at ball 442 With torsion no longer reaches connecting rod 440.

Moving mass 480 described in the present embodiment includes auxiliary section 482 and driving section 484, the lower end of the auxiliary section 482 and institute The upper end for stating driving section 484 connects to form T-shaped, and the upper end of moment of torsion regulating part 460 is provided with spacing hole, the auxiliary section 482 with The spacing hole is slidably matched, and the disc spring 470 is set in outside the auxiliary section 482 and disc spring 470 is connected to the driving section On 484, the lower surface of the driving section 484 is provided with the first groove, and it is recessed that the upper surface of the connecting rod 440 is provided with second Groove, the ball 442 are docked in the space to be formed with the second groove positioned at the first groove.Moving mass 480 by auxiliary section 482 with Moment of torsion regulating part 460 is slidably connected, and relative can move up and down relatively unrotatable, the spacing hole position mounting hole between the two, The auxiliary section 482 is the kidney-shaped structure coordinated with mounting hole, and according to the actual requirements, mating holes can also be that other are non-circular Hole, ball 442 are many, and when moving mass 480 is docked with 440 upper surface of connecting rod, ball 442 is recessed with second in the first groove Rotate in groove and can transmit torsion, when both separate, ball 442 no longer transmits torsion.

The moment of torsion regulating part 460 is threadedly coupled with the connector 430, and the upper end of the moment of torsion regulating part 460 is provided with Extension 462, offers adjustment hole 464 on the extension 462.Regulation instrument is stretched into band dynamic torque in adjustment hole 464 to adjust Section 460 opposite joints 430 of part are in rotary moving, adjust the size of disc spring 470 so as to control the size of screw capping period of the day from 11 p.m. to 1 a.m torsion.

The screw capping mechanism 40 also includes linear bearing 444, and the housing 420 includes the upper shell 420 being detachably connected With lower house 420, the linear bearing 444 is set in outside the connecting rod 440, and linear bearing 444 and the lower house 420 Connection, is provided with step site 434 in the connector 430, and the connecting rod 440 is spacing at described near one end of ball 442 In component level 434.Upper shell 420 and the lower house 420 that housing 420 is arranged to be detachably connected, is easy to Assembly And Disassembly, arranges Linear bearing 444 makes the rotation of connecting rod 440 more smooth, arranges step site 434, limited connecting rod 440 in connector 430 Position is in connector 430.

The screw capping mechanism 40 also includes connecting shaft 412, and the first side of the cantilever 10 is provided with and is connected with the transmission belt Fixed mount 110,412 one end of the connecting shaft is connected with the output shaft of the 3rd motor 410, the other end and the shell Body 420 connects, and the 3rd motor 410 is arranged on the fixed mount 110, the connecting shaft 412 and the fixed mount 110 rotate connection.The torsion of the 3rd motor 410 reaches housing 420, connecting shaft 412 and the fixed mount by connecting shaft 412 110 rotate connection, and fixed mount 110 is not interfered which to rotate, screw capping mechanism 40 is connected with cantilever 10 by fixed mount 110.

A kind of reference picture 1-4, cell centrifugation method, comprises the steps：

A, centrifugal turntable 20 turn to initial position with the sample rotating disk for being located at its periphery, make in sample rotating disk circumferential direction Sample bottle with 20 circumferential direction of centrifugal turntable centrifugation storehouse 210 position it is corresponding；

B, cantilever 10 drive screw capping mechanism 40 to be thereon moved to above sample rotating disk with sampling mechanism 50, screw capping mechanism 40 Decline is turned on the lid back boom 10 of correspondence position sample bottle and is moved, and sampling mechanism 50 declines in the sample bottle drawn after uncapping Sample, cantilever 10 are moved again, and sampling mechanism 50 is moved on corresponding with the current sample bottle centrifugation storehouse of centrifugal turntable 20 210 Side, in 50 times general who has surrendered's sample drops of sampling mechanism to the centrifuge tube 212 being centrifuged in storehouse 210；Cantilever 10 is moved again, screw capping mechanism 40 The sample bottle cover that lower general who has surrendered uncaps is tightened；

C, centrifugal turntable 20, sample rotating disk rotate special angle respectively, make sample bottle in sample rotating disk circumferential direction with The position correspondence in the centrifugation storehouse 210 in 20 circumferential direction of centrifugal turntable, and pending sample bottle and the centrifugation being centrifuged in storehouse 210 Pipe 212 is located in the range of 10 shift motion of cantilever；

D, repeat step b and step c, until the sample transfer on sample rotating disk is completed；

E, centrifugal turntable are actuated for centrifugal treating.

The cell centrifugation method, can be carried out using above-mentioned cytospin, drive screw capping mechanism 40 by cantilever 10 and take Model machine structure 50 is moved left and right, the sampling of screw capping, efficiency high and will not contaminated samples, while sample rotating disk and centrifugal turntable 20 rotate, and make untreated sample bottle and centrifugation storehouse 210 move to 10 correspondence position of cantilever, further improve liquid relief efficiency, realize Cell automatic centrifugation is operated.When the sample bottle on sample rotating disk is opening-wide state in itself, the step that need not be uncapped with locking closure Suddenly, the step of screw capping mechanism 40 twists the lid off and tightens lid wherein in step b can not be implemented, and sampling mechanism is directly taken Sample is operated.

Wherein, step e specifically includes following steps：

E1, centrifugal turntable 20 stop after the centrifugation scheduled time, and cantilever 10 is moved to above centrifugal turntable, under sampling mechanism 50 Drop draws the supernatant in centrifuge tube 212；

E2, centrifugal turntable 20 are again started up being centrifuged；

E3, repeat step e1, e2 are until reach expected centrifugal effect.

Imbibition operation is carried out automatically by sampling mechanism 50, loaded down with trivial details artificial operation is saved, centrifugal efficiency is higher.

Wherein, in step c, there is multiple rows of centrifuge tube 212 for placing sample in each centrifugation storehouse 210, when wherein one is discharged to After in heart pipe 212, drop has sample, sample rotating disk rotates predetermined angle, and centrifugal turntable 20 is not rotated, the sampling mechanism on cantilever 10 50 draw the sample in correspondence position sample bottle, and cantilever 10 is moved and for sample to drop to another row's centrifuge tube 212 in centrifugation storehouse 210 It is interior, there is sample ability rotating centrifugal rotating disk 20 until the current centrifuge tube 212 being centrifuged in storehouse 210 all drips, make 210 turns of another centrifugation storehouse Move in the range of 10 shift motion of cantilever.Centrifugal turntable 20, and centrifuge tube 212 and sample bottle can not only be more fully utilized so Accurate contraposition can be realized.

Wherein, in step a, c, with during sample rotating disk para-position, centrifugal turntable 20 adopts the second positioning motor to centrifugal turntable 20 Drive and rotate, second positioning motor is connected with the power transmission shaft of centrifugal turntable 20 by electromagnetic clutch 620；In step e, from Driven using centrifugation motor 610 during the heart 20 centrifugal treating of rotating disk, now the transmission of electromagnetic clutch 620 and the centrifugal turntable 20 Axle disconnects, and centrifugal turntable 20 is stopped after motor 610 is centrifuged to drive the scheduled time；Electromagnetic clutch 620 is turned with centrifugation again The power transmission shaft connection of disk 20, the second positioning motor drive centrifugal turntable 20 to rotate and make which with 50 para-position of sampling mechanism；Work as sampler After structure 50 has drawn the supernatant in centrifuge tube 212, electromagnetic clutch 620 is broken again with the power transmission shaft of the centrifugal turntable 20 Open, centrifugation motor 610 drives centrifugal turntable 20 to carry out centrifugal treating again.Centrifugal turntable 20 is realized using centrifugation motor 610 High speed centrifugation is operated, and is selectively attached with centrifugal turntable 20 using the second positioning motor, is realized the right of centrifugal turntable 20 Bit manipulation, while meet the demand of two kinds of different service conditions.

Each technical characteristic of embodiment described above arbitrarily can be combined, for making description succinct, not to above-mentioned reality Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, the scope of this specification record is all considered to be.

Embodiment described above only expresses several embodiments of the present utility model, and its description is more concrete and detailed, But therefore can not be interpreted as the restriction to this utility model the scope of the claims.It should be pointed out that common for this area For technical staff, without departing from the concept of the premise utility, some deformations and improvement can also be made, these all belong to In protection domain of the present utility model.Therefore, the protection domain of this utility model patent should be defined by claims.

Claims (10)

1. a kind of cytospin, it is characterised in that including cantilever, the centrifugal turntable for placing centrifugation storehouse and for placing sample The sample rotating disk of product bottle, the sample rotating disk are located at centrifugal turntable periphery, and the cantilever is located on centrifugal turntable and sample rotating disk Square and horizontally moveable, the cantilever is provided with sampling mechanism, and the sampling mechanism energy opposing cantilevers are moved up and down, the sample Multiple sample positions for placing sample bottle are provided with around its circumferential direction on product rotating disk.

2. cytospin as claimed in claim 1, it is characterised in that the sample rotating disk is driven by the first drive mechanism Dynamic, first drive mechanism includes the first positioning motor, Timing Belt and gear, the gear and first positioning motor Output shaft connects, and the Timing Belt is set in outside the sample rotating disk, and the Timing Belt outer wall is provided with and is nibbled with the gear The tooth of conjunction.

3. cytospin as claimed in claim 2, it is characterised in that the centrifugal turntable is driven by the second drive mechanism Dynamic, second drive mechanism includes being centrifuged motor, electromagnetic clutch and the second positioning motor, the centrifugation motor with it is described from The power transmission shaft connection of heart rotating disk, second positioning motor are connected with the power transmission shaft of the centrifugal turntable by electromagnetic clutch.

4. cytospin as claimed in claim 3, it is characterised in which also includes controlling organization, on the centrifugal turntable The first position-sensing switch is provided with, the sample rotating disk is provided with the second position-sensing switch, second positioning motor, the first positioning electricity Machine, the first position-sensing switch and the second position-sensing switch are electrically connected with the controlling organization respectively.

5. the cytospin as described in any one of Claims 1-4, it is characterised in that the sampling mechanism include pipe support and The probe tube being arranged on the pipe support, the cantilever are provided with the first motor and the transmission belt being longitudinally arranged, the biography One end of dynamic band is rotated with the rotary shaft of first motor and is connected, and the other end of transmission belt is wound on belt wheel, the pipe Frame is connected with the wherein side of the transmission belt.

6. cytospin as claimed in claim 5, it is characterised in that the sampling mechanism also includes pipette, the suction Liquid pipe is arranged on the pipe support, is additionally provided with multiple samplings for placing probe tube around its circumferential direction on the sample rotating disk Position and multiple avoidance positions for avoiding pipette, and the sample position, sampling position and avoidance position are located at sample rotating disk different half On the circumferencial direction in footpath, on the centrifugal turntable, multiple screens are provided with around its circumferential direction, the centrifugation storehouse can with the screens Dismounting connects, and the centrifugation storehouse can be rotated around the screens.

7. cytospin as claimed in claim 6, it is characterised in which also includes screw capping mechanism, the screw capping mechanism sets Put in the first side of cantilever, the sampling mechanism is arranged on the second side of cantilever, at the cantilever position relative with transmission belt Long through-hole is offered, the screw capping mechanism is connected with the transmission belt by the long through-hole, and screw capping mechanism is connected with transmission belt The side for connecing is oppositely arranged with the side that the sampling mechanism is connected with transmission belt, makes screw capping mechanism with sampling mechanism with transmission belt Mobile move towards different direction respectively.

8. cytospin as claimed in claim 7, it is characterised in which also includes workbench, the sample rotating disk, centrifugation Rotating disk is rotatably arranged on workbench, and the workbench is provided with bracing frame, and support frame as described above is provided with along sample rotating disk Or centrifugal turntable radial direction arrangement crossbeam, the cantilever by the second motor control be slidably disposed on crossbeam On, the crossbeam is provided with two cantilevers, each cantilever be provided with two screw capping mechanisms, two probe tubes and Two pipette, Liang Ge screw cappings mechanism are set up in parallel, and two probe tubes are set up in parallel, and two pipette are arranged side by side Arrange, and the spacing that is set up in parallel of two screw capping mechanisms, two probe tubes and two pipette and sample turn On disk, the spacing of neighboring samples bottle is equal.

9. cytospin as claimed in claim 4, it is characterised in that also include above sample rotating disk with centrifugal turntable Scanning device, the scanning device be used for scan centrifugation storehouse on bar code and/or the bar code on sample bottle and/or centrifugation storehouse in centrifugation Bar code on pipe, the scanning device are electrically connected with the controlling organization；Also include the protection for detecting centrifugation motor speed Device, the centrifugal turntable are externally provided with protective cover, when the protector detects the centrifugation motor just in the running, the protection Cover cannot be opened and the electromagnetic clutch cannot run；Also include for detecting that sample to be centrifuged on centrifugal turntable or so is The balanced detector of no balance, when detecting on centrifugal turntable when sample being centrifuged or so is uneven, balanced detector sends Warning is reminded.

10. cytospin as claimed in claim 7, it is characterised in that the first side of the cantilever is provided with and the transmission Fixed mount with connection, the screw capping mechanism include that the 3rd motor, connecting shaft, housing, connector, connecting rod, moment of torsion are adjusted Section part, disc spring, moving mass, unilateral bearing and the cover body matched with sample bottle cover, described connecting shaft one end is driven with the described 3rd The output shaft connection of galvanic electricity machine, the other end are connected with the housing, and the 3rd motor is arranged on the fixed mount, institute State connecting shaft and rotate with the fixed mount and be connected, the housing is provided with the chute along longitudinal direction, and the connector is provided with and stretches out Bar, connector are arranged in housing, and the extension bar is coordinated with the slide, is provided with and is connected to connector in the housing The elastic component of top, the unilateral bearing is arranged in the connector, and unilateral bearing is set in outside the connecting rod, unidirectionally The outer ring of bearing is connected with the connector, and the moment of torsion regulating part is threadedly coupled with the connector, the moment of torsion regulating part Upper end be provided with extension, offer adjustment hole on the extension, the disc spring and moving mass are arranged in moment of torsion regulating part, And one end of disc spring is connected on the inwall of moment of torsion regulating part, the other end is connected to the upper end of moving mass, under the moving mass End passes through ball engagement with the upper end of connecting rod, and the lower end of connecting rod is stretched out the housing and is connected with the upper surface of the cover body, Offer the holding tank matched with the sample bottle cover on the lower surface of the cover body, the sample bottle cover it is circumferentially-spaced Multiple chuck are provided with, multiple draw-in grooves matched with the chuck on the side wall of the holding tank, are offered.