CN106268384B - A kind of automatic dissolving device - Google Patents
A kind of automatic dissolving device Download PDFInfo
- Publication number
- CN106268384B CN106268384B CN201610902580.2A CN201610902580A CN106268384B CN 106268384 B CN106268384 B CN 106268384B CN 201610902580 A CN201610902580 A CN 201610902580A CN 106268384 B CN106268384 B CN 106268384B
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- dissolution
- instrument
- switch
- groove
- bulkhead union
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F21/00—Dissolving
- B01F21/30—Workflow diagrams or layout of plants, e.g. flow charts; Details of workflow diagrams or layout of plants, e.g. controlling means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
- B01F33/85—Mixing plants with mixing receptacles or mixing tools that can be indexed into different working positions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/2201—Control or regulation characterised by the type of control technique used
- B01F35/2209—Controlling the mixing process as a whole, i.e. involving a complete monitoring and controlling of the mixing process during the whole mixing cycle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
- B01F35/2211—Amount of delivered fluid during a period
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of automatic dissolving device, including dissolution unit, control unit and operation interface；Wherein, the dissolution unit includes dissolution instrument, reagent bottle and inert gas bottle, and the dissolution instrument is embedded with the time relay, switch and power switch；The dissolution instrument is equipped with multiple grooves, and the top of the groove is equipped with reagent input port；The dissolution instrument medial surface is equipped with precise pressure regulating valve, solenoid valve；The side of the dissolution instrument is equipped with air pressure instrument, and the air pressure instrument is threadedly coupled with the precise pressure regulating valve；Described control unit is connect with the time relay, switch, precise pressure regulating valve, solenoid valve control；Described control unit is connect with the operation interface.Automatic dissolving apparatus structure provided by the invention is simple and convenient to operate, high degree of automation, can be accurately controlled the flow of liquid.
Description
Technical field
The present invention relates to DNA detection, dissolution, synthesis device field more particularly to a kind of automatic dissolving devices.
Background technique
Traditional DNA synthesis material (phosphoramidite monomer) dissolving method is mainly molten using carrying out with graduated container
It solves, the flow speed of liquid is mainly to be obtained by the experience of long-term accumulated in course of dissolution, main present in course of dissolution
Sight factor is more, and the precision for resulting in dissolution is poor, and often ingress of air during dissolution makes on reagent moisture
It rises, thus will affect the precision of later period DNA/RNA synthesis.In addition, traditional dissolving method can only carry out single port dissolution, carrying out
When batch dissolves, complicated for operation, accuracy is low, time-consuming and laborious.
Summary of the invention
In view of the deficiencies in the prior art, the present invention provides a kind of automatic dissolving device, and structure is simple, operation side
Just, high degree of automation can be accurately controlled the flow of liquid.
In view of the above technical problems, the technical solution adopted by the present invention is that, the present invention provides a kind of automatic dissolving device,
Including dissolution unit, control unit and operation interface；The dissolution unit includes dissolution instrument, reagent bottle and inert gas bottle, institute
Dissolution instrument is stated embedded with the time relay, switch and power switch；The dissolution instrument is equipped with groove, and the top of the groove is equipped with
Reagent input port；The size of the bottle cap and the caliber size of container match, it is ensured that in reagent course of dissolution
It is not contacted with air, maintains original moisture in reagent, while avoiding the increase of moisture in reagent；
The dissolution instrument medial surface is equipped with precise pressure regulating valve, solenoid valve；The side of the dissolution instrument is equipped with air pressure instrument, institute
Air pressure instrument is stated to be threadedly coupled with the precise pressure regulating valve；
Described control unit is connect with the time relay, switch, precise pressure regulating valve, solenoid valve control；
Described control unit is connect with the operation interface.
Preferably, the quantity value of groove is 1-20, can be equipped with container in each groove, it is same to can be realized 1-20 container
Shi Jinhang dissolution.
Preferably, the time relay, switch, power switch are arranged successively from top to bottom, and the switch includes quantitative
Volume switch and non-quantitative volume switch, and the determined volume switch is arranged up and down with non-quantitative volume switch.
Preferably, the side of the dissolution instrument is successively arranged the first BULKHEAD UNION, the second BULKHEAD UNION, third from top to bottom
BULKHEAD UNION, first BULKHEAD UNION, the second BULKHEAD UNION, third BULKHEAD UNION 1/4 pass through dissolution instrument.
Preferably, first BULKHEAD UNION, the second BULKHEAD UNION are set to the top of the air pressure instrument, and the third is worn
Plate connector is set to the lower section of the air pressure instrument.
Preferably, the dissolution unit further includes reagent bottle and inert gas bottle, one end of the third BULKHEAD UNION with
The gas output tube of the inert gas bottle connects, the lower end piping connection of the other end and precise pressure regulating valve.
Preferably, the upper end piping connection of one end of second BULKHEAD UNION and precise pressure regulating valve, the other end and reagent
The gas inlet pipe connection of bottle.
Preferably, one end of first BULKHEAD UNION is connect with the reagent efferent duct of the reagent bottle, the other end and institute
One end piping connection of solenoid valve is stated, the other end of the solenoid valve is connected by the reagent input port on pipeline and the groove
It connects.
Preferably, the reagent in the reagent bottle is anhydrous organic solvent, preferably anhydrous acetonitrile.
Preferably, the adjusting pressuring knob, temperature sensor and humidity sensor of precise pressure regulating valve are additionally provided on the dissolution instrument,
The temperature sensor and humidity sensor are connect with control unit；The temperature sensor and humidity sensor are for detecting
The temperature and humidity of atmosphere avoids influence of the temperature and humidity in atmosphere to course of dissolution；The pressure regulation of the precise pressure regulating valve
Button is connect with the precise pressure regulating valve.
Preferably, the another side of the dissolution instrument is equipped with power outlet.
Preferably, described control unit is using any in PLC control, relay control or single-chip microcontroller control.
Preferably, the operation interface uses touch LCD screen interface or computer operation interface.
Preferably, the depth value of the groove is 100mm-200mm, and the front of the groove is divided into rectangle structure,
The rear portion of the groove is divided into semicircular structure, is more suitable for the placement of container.
Preferably, the liquid volume value range 5ml-400ml that the dissolution instrument is got.
The method that DNA synthesis material (phosphoramidite monomer) is dissolved using above-mentioned automatic dissolving device, including such as
Lower step:
(1) startup power supply opening operation interface, control unit, and press the power switch on dissolution instrument；
(2) open inert gas bottle switch, adjustment precision pressure regulator valve one constant pressure of pressure regulation button settings (such as
6PSI)；
(3) opening time relay carries out flux modification by time and volume；
(4) time of volume needed for setting (such as 200 milliliters of volume needs 40 seconds time)
(5) required container opening lid is put into groove, the bottleneck of container and the reagent input port of groove top are opposite
It answers；
(6) it presses and starts to switch, dissolution instrument beats liquid until the time of setting automatically, dissolves instrument and stops working, completes dissolution.
Start button is pressed in above-mentioned (6) step as opening on the start button or dissolution instrument front end face in operation interface
It closes, if determined volume, then presses determined volume switch；If non-quantitative volume, then non-quantitative volume switch, Ke Yishi are pressed
Conversion and dissolution between existing determined volume and non-quantitative volume.
It compares with the prior art, the beneficial effect comprise that:
(1) automatic dissolving device provided by the invention, structure is simple, easy to operate, high degree of automation, time saving and energy saving；
(2) automatic dissolving device provided by the invention uses the time relay, can be realized quantitative liquid feeding body, accurate to control
The flow of liquid is made, dissolution control precision is high；
(3) caliber size of the size of the bottle cap on automatic dissolving device further groove top provided by the invention and container
Match, during dissolution, cooperation is fully sealed in bottle cap and vessel port, is avoided liquid-to-air contact and is increased reagent
The phenomenon that moisture；
(4) single port dissolution not only may be implemented in dissolver provided by the invention, can also realize more mouthfuls of dissolutions, improve
Working efficiency has saved cost of labor.
Detailed description of the invention
Fig. 1 is the schematic diagram of automatic dissolving device provided by the invention；
Fig. 2 is the structural schematic diagram of single port dissolution unit provided by the invention；
Fig. 3 is the structural schematic diagram (one) of single port dissolution instrument provided by the invention；
Fig. 4 is the schematic diagram of internal structure of single port dissolution instrument provided by the invention；
Fig. 5 is the structural schematic diagram (two) of single port dissolution instrument provided by the invention；
Fig. 6-7 is more mouthfuls of dissolutions cell schematics provided by the invention；
The concrete meaning of each label is as follows in attached drawing:
1: dissolution instrument；2: the time relay；3: switch；31: quantitative gas switch；32: non-quantitative gas switch；4: power supply
Switch；5: reagent input port；6: container；7: the first BULKHEAD UNIONs；8: the second BULKHEAD UNIONs；9: third BULKHEAD UNION；10: air pressure
Instrument；11: precise pressure regulating valve；12: solenoid valve；13: relay；14: pipeline；15: adjusting pressuring knob；16: power outlet；17: recessed
Slot；18: reagent bottle；19: inert gas bottle；20: operation interface；21: control unit.
Specific embodiment
With reference to the accompanying drawing, detailed elaboration is made to the specific embodiment in the present invention.
Join shown in Fig. 1, Fig. 1 is the schematic diagram of automatic dissolving device provided by the invention, wherein automatic dissolving device includes
Dissolve unit, control unit 21 and operation interface 20；The input terminal of control unit 21 is connect with dissolution unit, control unit 21
Output end is connect with operation interface 20；Control unit 21 is using any one of PLC control, relay control or single-chip microcontroller control
Control mode；Operation interface 20 uses touch LCD screen interface or computer operation interface；
Join shown in Fig. 2-4, Fig. 2 is the schematic diagram that single port dissolves unit in the present invention, wherein it includes molten that single port, which dissolves unit,
Solve instrument 1, reagent bottle 18 and inert gas bottle 19；Fig. 3 is the structural schematic diagram for dissolving instrument, wherein the front end face of dissolution instrument 1 is from upper
The time relay 2, switch 3 and power switch 4 are successively embedded under；Switch is divided into quantitative gas switch 31 and non-quantitative gas is opened
Close 32；The front end face of dissolution instrument 1 is additionally provided with groove 17, and the top of groove 17 is equipped with reagent input port 5；The side of instrument is dissolved from upper
The first BULKHEAD UNION 7, the second BULKHEAD UNION 8, air pressure instrument 10, third BULKHEAD UNION 9, the first BULKHEAD UNION are successively arranged under
7, the second BULKHEAD UNION 8, third BULKHEAD UNION 9 1/4 pass through dissolution instrument；The front of groove 17 is divided into rectangle structure, rear portion
It is divided into semicircular structure；
Fig. 4 be in the present invention single port dissolve instrument schematic diagram of internal structure, wherein dissolution instrument in be equipped with precise pressure regulating valve 11,
Solenoid valve 12, wherein precise pressure regulating valve 11 is threadedly coupled with air pressure instrument 10；The time relay 2, switch 3, precise pressure regulating valve 11
It controls and connects with control unit with solenoid valve 12；
One end of first BULKHEAD UNION 7 is connect with the reagent efferent duct of reagent bottle 18, one end of the other end and solenoid valve 12
The other end of piping connection, solenoid valve 12 is connect by pipeline with the reagent input port 5 on groove；
One end of second BULKHEAD UNION 8 and the upper end piping connection of precise pressure regulating valve 11, the gas of the other end and reagent bottle
Input pipe connection；
One end of third BULKHEAD UNION 9 is connect with the gas output tube of inert gas bottle 19, the other end and precise pressure regulating valve
11 lower end piping connection；
Shown in ginseng Fig. 5, the back side for dissolving instrument is additionally provided with the pressure regulation button 15 of precise pressure regulating valve, temperature sensor and humidity and passes
Sensor, temperature sensor and humidity sensor are connect with control unit；The another side for dissolving instrument is equipped with power outlet 16.
Fig. 6-Fig. 7 is another embodiment of the invention, i.e., more mouthfuls dissolution cell schematics, more mouthfuls of dissolution units can be real
Existing multiple containers are dissolved simultaneously, improve dissolved efficiency.
When carrying out DNA/RNA dissolution using automatic dissolving device provided by the invention, startup power supply first, opening operation circle
Face, control unit, and the power switch on dissolution instrument is pressed, starting dissolution instrument；Then inert gas bottle switch is opened, essence is adjusted
One constant pressure of pressure regulation button settings of close pressure regulator valve；The time relay is again turned on to carry out by regulating time and volume
Flux modification, the time of volume needed for setting；The container that dissolves will be needed to open lid to be put into groove, the bottleneck of container with it is recessed
The reagent input port in groove top portion is corresponding；
It presses and starts to switch, dissolution instrument beats liquid until the time of setting automatically, dissolves instrument and stops working, completes DNA dissolution.
A series of detailed description illustrating only for feasible embodiment of the invention described above,
They are not to limit the scope of the invention, in the knowledge that the technical field those of ordinary skill has
Interior, the various change made without departing from the purpose of the present invention all belongs to the scope of protection of the present invention.
Claims (8)
1. a kind of automatic dissolving device, including dissolution unit, control unit and operation interface；It is characterized in that, the dissolution is single
Member includes dissolution instrument, reagent bottle and inert gas bottle, and the dissolution instrument is embedded with the time relay, switch and power switch；It is described
The time relay, switch, power switch are arranged successively from top to bottom, and the dissolution instrument is equipped with multiple grooves, the top of the groove
Portion is equipped with reagent input port；
The medial surface of the dissolution instrument is equipped with precise pressure regulating valve, solenoid valve；
The side of the dissolution instrument is equipped with air pressure instrument, and the air pressure instrument is threadedly coupled with the precise pressure regulating valve；
Described control unit is connect with the time relay, switch, precise pressure regulating valve, solenoid valve control；
Described control unit is connect with the operation interface；
The side of the dissolution instrument is successively arranged the first BULKHEAD UNION, the second BULKHEAD UNION, third BULKHEAD UNION, institute from top to bottom
State the first BULKHEAD UNION, the second BULKHEAD UNION, third BULKHEAD UNION 1/4 pass through dissolution instrument；
First BULKHEAD UNION, the second BULKHEAD UNION are set to the top of the air pressure instrument, and the third BULKHEAD UNION is set to
The lower section of the air pressure instrument；
The dissolution unit further includes reagent bottle and inert gas bottle, one end and the inert gas of the third BULKHEAD UNION
The gas output tube connection of bottle, the lower end piping connection of the other end and precise pressure regulating valve；
The gas of one end of second BULKHEAD UNION and the upper end piping connection of precise pressure regulating valve, the other end and reagent bottle inputs
Pipe connection；
One end of first BULKHEAD UNION is connect with the reagent efferent duct of the reagent bottle, and the one of the other end and the solenoid valve
Piping connection is held, the other end of the solenoid valve is connect by pipeline with the reagent input port on the groove.
2. automatic dissolving device as described in claim 1, which is characterized in that the quantity value of the groove is 1-20, each
Groove is built-in with container, can be realized 1-20 container while being dissolved.
3. automatic dissolving device as described in claim 1, which is characterized in that it is described switch include determined volume switch and it is indefinite
Volume switch is measured, the determined volume switch switchs arrangement up and down with the non-quantitative volume.
4. automatic dissolving device as described in claim 1, which is characterized in that the reagent in the reagent bottle is anhydrous organic molten
Agent.
5. automatic dissolving device as described in claim 1, which is characterized in that the dissolution instrument is equipped with the pressure regulation of precise pressure regulating valve
Knob, temperature sensor and humidity sensor, the temperature sensor and humidity sensor are connect with control unit；It is described molten
The another side of Xie Yi is equipped with power outlet.
6. automatic dissolving device as described in claim 1, which is characterized in that described control unit is using PLC control, relay
It is any in control or single-chip microcontroller control；The operation interface uses touch LCD screen interface or computer operation interface.
7. automatic dissolving device as described in claim 1, which is characterized in that the depth value of the groove is 100mm-
200mm, the front of the groove are divided into rectangle structure, and the rear portion of the groove is divided into semicircular structure；The dissolution instrument is beaten
Liquid volume value range 5ml-400ml out.
8. the method for carrying out the dissolution of DNA synthesis material using the automatic dissolving device as described in any in claim 1-7, special
Sign is, includes the following steps:
(1) startup power supply opening operation interface, control unit, and press the power switch on dissolution instrument；
(2) inert gas bottle switch, one constant pressure of pressure regulation button settings of adjustment precision pressure regulator valve are opened；
(3) opening time relay carries out flux modification by time and volume；
(4) time of volume needed for setting；
(5) required container opening lid is put into groove, the bottleneck of container and the reagent input port of groove top are corresponding；
(6) starting to switch on pushing operation interface, dissolution instrument beats liquid until the time of setting automatically, and it dissolves instrument and stops working,
Complete dissolution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610902580.2A CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610902580.2A CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106268384A CN106268384A (en) | 2017-01-04 |
| CN106268384B true CN106268384B (en) | 2019-01-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610902580.2A Active CN106268384B (en) | 2016-10-17 | 2016-10-17 | A kind of automatic dissolving device |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111949058B (en) * | 2020-07-29 | 2022-02-08 | 北京擎科生物科技有限公司 | Linkage control system and method for indoor temperature and humidity in DNA synthesizer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2048186C1 (en) * | 1992-01-22 | 1995-11-20 | Александр Александрович Ковалев | Assembly for preparing and metering components |
| CN2748160Y (en) * | 2003-11-18 | 2005-12-28 | 张�林 | Automatic dry medicament powder dissolving system |
| CN201260940Y (en) * | 2008-09-01 | 2009-06-24 | 四川电力试验研究院 | Separated flow type standard humidity generator |
| CN203247079U (en) * | 2013-05-17 | 2013-10-23 | 温州龙华日用电子有限公司 | Pneumatic high-precision automatic quantitative liquid divider |
| CN204121989U (en) * | 2014-10-15 | 2015-01-28 | 江苏天河水务设备有限公司 | Two van-type automatic medicine adding apparatus |
-
2016
- 2016-10-17 CN CN201610902580.2A patent/CN106268384B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2048186C1 (en) * | 1992-01-22 | 1995-11-20 | Александр Александрович Ковалев | Assembly for preparing and metering components |
| CN2748160Y (en) * | 2003-11-18 | 2005-12-28 | 张�林 | Automatic dry medicament powder dissolving system |
| CN201260940Y (en) * | 2008-09-01 | 2009-06-24 | 四川电力试验研究院 | Separated flow type standard humidity generator |
| CN203247079U (en) * | 2013-05-17 | 2013-10-23 | 温州龙华日用电子有限公司 | Pneumatic high-precision automatic quantitative liquid divider |
| CN204121989U (en) * | 2014-10-15 | 2015-01-28 | 江苏天河水务设备有限公司 | Two van-type automatic medicine adding apparatus |
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| CN106268384A (en) | 2017-01-04 |
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Effective date of registration: 20190412 Address after: 100176 Beijing Daxing District Beijing Economic and Technological Development Zone 2 Floor 2, No. 25 Courtyard, Jinghai Fourth Road Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 102209 Xisha Industrial Park No. 1, North Qijia Town, Changping District, Beijing Patentee before: BEIJING DNACHEM BIOTECHNOLOGY CO.,LTD. |
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Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 100176 Beijing Daxing District Beijing Economic and Technological Development Zone 2 Floor 2, No. 25 Courtyard, Jinghai Fourth Road Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |
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