CN106244426A - High density Large-scale microalgae culture apparatus - Google Patents
High density Large-scale microalgae culture apparatus Download PDFInfo
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- CN106244426A CN106244426A CN201610754766.8A CN201610754766A CN106244426A CN 106244426 A CN106244426 A CN 106244426A CN 201610754766 A CN201610754766 A CN 201610754766A CN 106244426 A CN106244426 A CN 106244426A
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- raceway pond
- described raceway
- microalgae
- high density
- culture apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/18—Open ponds; Greenhouse type or underground installations
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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Abstract
The present invention relates to a kind of micro-algae large-scale culture apparatus, particularly high density Large-scale microalgae culture apparatus, including raceway pond, described raceway pond ovalize, described raceway pond center is provided with a dividing plate, is provided with a dewatering outlet bottom described raceway pond；It is provided with bottom described raceway pond by the evenly distributed submarine light formed of some fluorescent tubes, in described raceway pond, is provided with two culture fluid circulation power pumps.After using said structure, compared with prior art, high density Large-scale microalgae culture apparatus is sufficiently mixed culture fluid, makes microalgae can carry out sufficient illumination；Meanwhile, phase microalgae density is big after incubation, and illumination can carry out light filling with daylight lamp not, reaches highdensity cultivation.
Description
Technical field
The present invention relates to a kind of micro-algae large-scale culture apparatus, particularly high density Large-scale microalgae culture apparatus.
Background technology
Microalgae is as unicellular organism, and it has the faster speed of growth than terrestrial plant, microalgae can effectively utilize luminous energy,
CO2With inorganic salts synthetic protein, fat, carbohydrate and multiple high added value bioactive substance, can pass through
Microdisk electrode produces health food, food additive, feedstuff and other chemicals.With the modem industrial plants mode of production,
On a large scale, low cost, efficiently, the acquisition micro algae biomass of high-quality stable be premise.
The microdisk electrode form of current industrialized production application is open culture pond.Suction due to microalgae cell itself
Light and interception, the illumination degree of depth in open pond decays rapidly with the culture pond degree of depth and microalgae density, and whole water body presents
Go out highlights, surface (general only several millimeters or several centimetres, change), low light level district, middle level with intensity of illumination and frustule concentration
Light and shade partitioned organization with bottom no light zone.Big portion, middle and lower part frustule is in the low light level or dark space, it is impossible to obtain sufficient illumination,
Cell photosynthesis is more weak.Simultaneously as pedal agitating device is difficult to culture fluid along optical path direction (above-below direction)
Being sufficiently mixed, the microalgae illumination of different depth is unreasonable, and the microalgae cell being in culture pond top rests on intense light irradiation for a long time
The surface penetrated, illumination is superfluous, and the frustule of the middle and lower part low light level or dark region is difficult to arrive top layer, top by mixing and accepts light
According to, therefore to cultivate the efficiency of light energy utilization low for culture pond, and cell growth is slow, and area yield (refers to that unit takes up an area newly-increased microalgae face every day
Biomass) low, the most only 5-15g/m2/ d, causes large-scale culture floor space excessive.
The People's Republic of China (PRC) discloses a kind of utility model patent, Authorization Notice No.: CN 201110219548.A kind of
Micro algae culturing device, including: an open raceway pond, there is a liquid outlet, this liquid outlet connects a liquid circulation pump, logical
Cross this liquid circulation pump and connect the liquid-inlet of culture fluid basin；This culture fluid tank top has a liquid outlet conduit, makes training
Nutrient solution can flow back to open raceway pond through this pipeline.This is practical newly has the disadvantage in that
This utility model patent still uses pedal agitating device, culture fluid can not the mixing of above-below direction, meanwhile, volume
Much outer circulating pump and culture fluid storage fill, and increase cost.Installation fluorescent tube useless in device, it is not possible to carry out light filling, limits
The speed of growth of microalgae.
Summary of the invention
The invention reside in and a kind of simple in construction, the convenient highdensity micro algae culturing device of scale cleaned are provided.
For solving above-mentioned technical problem, the high density Large-scale microalgae culture apparatus of the present invention, including raceway pond, described race
Pond, road ovalize, described raceway pond center is provided with a dividing plate, is provided with a dewatering outlet bottom described raceway pond；At the bottom of described raceway pond
Portion is provided with by the evenly distributed submarine light formed of some fluorescent tubes, is provided with two culture fluid circulation powers in described raceway pond
Pump.
Further, described raceway pond upper end is uncovered, is respectively provided with a gas between described two ends, dividing plate left and right and raceway pond
Body distributor, described gas distributor is connected with ventilation pump by trachea.
Further, quartz heating rod it is provided with in described raceway pond.
After using said structure, compared with prior art, high density Large-scale microalgae culture apparatus is sufficiently mixed culture fluid,
Make microalgae can carry out sufficient illumination；Meanwhile, phase microalgae density is big after incubation, and illumination can be mended with daylight lamp not
Light, reaches highdensity cultivation.
Accompanying drawing explanation
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
Fig. 1 is the structural representation of high density Large-scale microalgae culture apparatus.
In figure: 1 be raceway pond, 2 for dividing plate, 3 for culture fluid circulation power pump, 4 for gas distributor, 5 for submarine light, 6
For dewatering outlet, 7 be quartz heating rod, 8 be ventilation pump, 9 for trachea
Detailed description of the invention
As it is shown in figure 1, the high density Large-scale microalgae culture apparatus of the present invention, including raceway pond 1, described raceway pond 1 in
Ellipse, described raceway pond 1 is uncovered.It is provided with a dividing plate 2 in the middle of described raceway pond 1, is provided with one bottom described raceway pond 1 and puts
The mouth of a river 6, is provided with bottom described raceway pond 1 by the evenly distributed submarine light 5 formed of some fluorescent tubes, adjustable intensity of illumination.
Described raceway pond is provided with vertically places quartz heating rod 7, it is ensured that the temperature of culture fluid will not be the lowest.It is provided with in described raceway pond 1
Two culture fluid circulation power pumps 3, drive culture fluid flowing.It is respectively provided with one between described dividing plate about 2 two ends and raceway pond 1
Gas distributor 4, described gas distributor 4 is connected with ventilation pump 8 by trachea 9.
The medium depth of middle-high density Large-scale microalgae culture apparatus raceway pond of the present invention controls at 15-25cm, this
Bright high density Large-scale microalgae culture apparatus changes the alr mode of culture fluid and with the addition of the light filling that fluorescent tube is extra, solves
Microalgae cell illumination of having determined is insufficient and illumination not enough, hence it is evident that improves microalgae cell in open raceway pond and produces speed
Degree, cell concentration and culture efficiency, it will help solve efficiency/cost (invest, the run) bottleneck of microalgae large-scale culture.
Use two a length of 2 meters in present embodiment, (culture area is 1.8 to the open raceway pond of ellipse of a width of 1 meter
Square metre) carry out SPIRULINA CULTIVATION, to compare explanation.
One of them raceway pond culture fluid height is 0.15m (cumulative volume 270 liters), and ventilation is 0.3VVM, beforehand control
Pump capacity 12m3/ h, the later stage can increase flow, and 16h illumination every day, 8h is dark, and night stops circulation.In cultivation cycle, flat
All temperature is about 25-30 DEG C.Initial frustule inoculum density is 0.4g/L, and after cultivating 15d, sample analysis, frustule concentration is
3.5g/L, equivalent area yield is (cultivating end of a period frustule concentration-Initial seeding density) * culture fluid cumulative volume (liter)/cultivation
Natural law/culture pond area=(3.5-0.4) * 270/15/1.8=31g/m2/d。
Another open raceway pond uses tradition training method as comparison, and culture fluid height is 0.15m (cumulative volume
270 liters), ventilation is 0.3VVM, and pedal stirring water velocity is 0.25 meter per second, and initial frustule inoculum density is 0.5g/
L, after cultivating 15d, sample analysis, frustule concentration is 1.9g/L, and equivalent area yield is (cultivation end of a period frustule concentration-first
Beginning inoculum density) * culture fluid cumulative volume (liter)/cultivated days/culture pond area=(1.9-0.5) * 270/15/1.8=14g/
m2/d。
The microalgae area yield of two kinds of method result of the comparison explanation present invention improves 121.4%.
In above example, algae kind used is replaced with scenedesmus, chlorella, Chaetoceros, Phaeodactylum, chrysophyceae or salt
Algaes etc., repeat above-mentioned experiment, and acquired results is similar.
Although the foregoing describing the detailed description of the invention of the present invention, but those skilled in the art should be appreciated that this
It is merely illustrative of, present embodiment can be made various changes or modifications, without departing from principle and the essence of the present invention,
Protection scope of the present invention is only limited by the claims that follow.
Claims (3)
1. a high density Large-scale microalgae culture apparatus, including raceway pond (1), is characterized in that: described raceway pond (1) is in ellipse
Shape, described raceway pond center is provided with a dividing plate (2), is provided with a dewatering outlet (6) bottom described raceway pond；Set bottom described raceway pond
It is equipped with by the evenly distributed submarine light (5) formed of some fluorescent tubes, in described raceway pond, is provided with two culture fluid circulation powers
Pump (3).
High density Large-scale microalgae culture apparatus the most according to claim 1, is characterized in that: described raceway pond (1) upper end
Uncovered, it is respectively provided with a gas distributor (4) between described dividing plate (2) two ends, left and right and raceway pond, described gas distributor leads to
Cross trachea (9) to be connected with ventilation pump (8).
High density Large-scale microalgae culture apparatus the most according to claim 1, is characterized in that: described raceway pond (1) is inner to be set
There is quartz heating rod (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610754766.8A CN106244426A (en) | 2016-08-27 | 2016-08-27 | High density Large-scale microalgae culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610754766.8A CN106244426A (en) | 2016-08-27 | 2016-08-27 | High density Large-scale microalgae culture apparatus |
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| Publication Number | Publication Date |
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| CN106244426A true CN106244426A (en) | 2016-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610754766.8A Pending CN106244426A (en) | 2016-08-27 | 2016-08-27 | High density Large-scale microalgae culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723214A (en) * | 2017-11-13 | 2018-02-23 | 烟台大学 | Plain type raceway pond and its application method for microdisk electrode |
| CN110257236A (en) * | 2019-06-25 | 2019-09-20 | 哈尔滨工业大学（深圳） | A kind of system of photoconductive tube-raceway pond combination culture oil-producing microalgae |
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|---|---|---|---|---|
| DE202014006168U1 (en) * | 2014-08-04 | 2014-10-09 | Ökotec-Anlagenbau Gmbh | Plant for rearing and reproduction of spirulina algae |
| CN104726321A (en) * | 2015-04-15 | 2015-06-24 | 中国海洋大学 | Runway type bioreactor applicable to sunshine factorization |
| CN105331530A (en) * | 2014-08-17 | 2016-02-17 | 王金鹤 | Novel biological detection device |
| CN105462816A (en) * | 2015-12-09 | 2016-04-06 | 重庆大学 | Raceway pond microalgae reactor for realizing frequency-division uniform distribution of sunlight by utilizing nano light guide plates |
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-
2016
- 2016-08-27 CN CN201610754766.8A patent/CN106244426A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202014006168U1 (en) * | 2014-08-04 | 2014-10-09 | Ökotec-Anlagenbau Gmbh | Plant for rearing and reproduction of spirulina algae |
| CN105331530A (en) * | 2014-08-17 | 2016-02-17 | 王金鹤 | Novel biological detection device |
| CN104726321A (en) * | 2015-04-15 | 2015-06-24 | 中国海洋大学 | Runway type bioreactor applicable to sunshine factorization |
| CN105462816A (en) * | 2015-12-09 | 2016-04-06 | 重庆大学 | Raceway pond microalgae reactor for realizing frequency-division uniform distribution of sunlight by utilizing nano light guide plates |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723214A (en) * | 2017-11-13 | 2018-02-23 | 烟台大学 | Plain type raceway pond and its application method for microdisk electrode |
| CN110257236A (en) * | 2019-06-25 | 2019-09-20 | 哈尔滨工业大学（深圳） | A kind of system of photoconductive tube-raceway pond combination culture oil-producing microalgae |
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