CN105331530A - Novel biological detection device - Google Patents
Novel biological detection device Download PDFInfo
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- CN105331530A CN105331530A CN201410402665.5A CN201410402665A CN105331530A CN 105331530 A CN105331530 A CN 105331530A CN 201410402665 A CN201410402665 A CN 201410402665A CN 105331530 A CN105331530 A CN 105331530A
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Abstract
The invention relates to a novel biological detection device. The novel biological detection device is provided with a photoreactor tank which is a vertical cylinder in shape, the ratio of the diameter to the height of the cylinder is 1 to 2.5, the cylinder is provided with a feeding port, a discharging port, a central manhole, observation holes, a gas outlet, a sensor interface and a sampling hole, the feeding port and the gas outlet are formed in the upper side faces of the cylinder respectively, the observation holes are formed in the cylinder vertically, the sensor interface is formed in the middle of the cylinder, and a sensor is arranged inside the cylinder. The novel biological detection device has the advantages that the novel biological detection device which is totally closed is capable of controlling parameters of illumination, temperature, pH (potential of hydrogen) value, dissolved oxygen, nutritive salt and the like freely; a light source which is arranged in the novel biological detection device can be used in a centralized manner; a stirring mechanism is advanced, and the novel biological detection device is simple in structure and quite low in production cost.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of new bio proofing unit.
Background technology
Biological monitor is the important tool of biotechnology.Micro-algae industry is new industry fast-developing at present, various different micro-algae can provide novel high-quality protein product, healthcare product, pharmaceutical prod and energy product etc., but micro-algae large-scale is cultivated and is mainly carried out at the raceway pond of open type at present, its shortcoming is a lot: raceway pond is difficult to avoid bacterium, fungi, other rapid-result assorted algaes and protozoic pollution and predation, and have a strong impact on quality; Because the change of seasonal climate and the natural condition such as is round the clock on the impact of output; Raceway pond will take a large amount of soils, and is subject to the impact of surrounding environment.Large-scale runway pond is cultivated, and because illumination, temperature etc. are non-constant, manages very difficult.Above-mentioned four, become the restraining factors that micro-algae is enhanced production capacities further and industry is produced, be therefore a kind ofly applied to one of enclosed bioreactor necessary requirement becoming large-scale production of industrialization Large Copacity.Current domestic and international totally-enclosed bioreactor mainly contains the types such as column, flat and tubular type, is middle-size and small-size, the requirement that external control light source is difficult to illumination control, temperature is difficult to reach industrialization.。
summary of the invention
Instant invention overcomes the deficiencies in the prior art, propose a kind of new bio proofing unit, described reactor is totally-enclosed, avoids the impact of above-mentioned all factors, illumination, temperature, PH, dissolved oxygen, nutritive salt etc. can freely control, and can avoid the pollution of bacterium, miscellaneous bacteria, protista completely.Built-in light source, that can concentrate utilizes light source, and light source is all utilized, and avoids the defect of light source diffusion or light pollution.Aerosol flexibility stirs, and without mechanical spiral slurry agitator, can unite two into one by aeration and agitation, and simultaneously light source can free setting, not by the impact of agitator.Compare with universal bio-reactor, structure is simple, and cost is lower.Highly versatile, also can be used as universal bio-reactor where necessary and uses.
Technical scheme of the present invention is: a kind of new bio proofing unit, be provided with photoreactor tank body, the profile of photoreactor tank body is upright cylindrical cylindrical shell, cylindrical tube diameter is 1:2.5 with the ratio of height, cylindrical tube is provided with opening for feed, discharge port, central authorities' manhole, vision slit, air outlet, sensor interface and thief hole, described opening for feed and air outlet are separately positioned on the upper side of cylindrical tube, vision slit is vertical array on cylindrical tube, sensor interface is arranged on the medium position of cylindrical tube, the inside of cylindrical tube is provided with sensor, sensor interface is connected with sensor, sensor comprises pressure transmitter and temperature sensor, described pressure transmitter is connected with aerosol whipped-up structure by signal wire with temperature sensor, discharge port is arranged on the bottom position of cylindrical tube, light beam support and aerosol whipped-up structure is provided with in cylindrical tube inside, described light beam support level is arranged on the medium position of cylindrical tube, aerosol whipped-up structure is horizontally set on the bottom of cylindrical tube, upper cover is provided with above cylindrical tube, upper cover is provided with above upper cover, described light beam support comprises cross frame and ring support, ring support is rounded, hole is penetrated at the light beam being circumferentially provided with equidistantly arrangement of ring support, described cross frame and ring support welding are in one plane, described upper cover is provided with annular cover plate and described cross frame, the excircle of annular cover plate is provided with described light beam and penetrates hole, the center of annular cover plate is described central manhole, annular cover plate and the welding of described cross frame are in one plane, penetrate in hole at described light beam and be installed with light beam, the top of described light beam is connected with a power lead by lamppost wire, described power lead is connected with a photovoltaic controller, described photovoltaic controller is arranged in described central manhole, aerosol whipped-up structure comprises system control unit, micro-porous sparger, anchor and adapter, described micro-porous sparger is looped around on described anchor, described adapter is connected with the initial end of micro-porous sparger, snakelike heating coil is circumferentially wound with at cylindrical tube, described snakelike heating coil is connected with aerosol whipped-up structure.
The center of described anchor is provided with a set collar, and at the some scattering supports of circumferentially equidistant welding of set collar, described scattering support is outside scattering centered by the center of circle of set collar, and described scattering support and set collar are in one plane.
Described light beam comprises Glass tubing, light bar wire and light bar, and described light bar is arranged on glass tube, and described light bar wire is connected with light bar, and described light bar is made up of the vertical arranged in series of LED together.
the present invention has following beneficial effect:
The present invention is totally-enclosed, most parameter as, illumination, temperature, PH, dissolved oxygen, nutritive salt etc. can freely control, and can avoid the pollution of bacterium, miscellaneous bacteria, protista completely.Built-in light source, that can concentrate utilizes light source, avoids the defect of light source diffusion or light pollution.Aerosol flexibility stirs, and without mechanical spiral slurry agitator, can unite two into one by aeration and agitation, and simultaneously light source can free setting, not by the impact of agitator.Compare with universal bio-reactor, structure is simple, and cost is lower.
Accompanying drawing illustrates:
Fig. 1 is external structure schematic diagram of the present invention.
Fig. 2 is light beam structural representation of the present invention.
Fig. 3 is aerosol whipped-up structure schematic diagram of the present invention.
Fig. 4 is internal structure schematic diagram of the present invention.
Fig. 5 is upper seal head structure schematic diagram of the present invention.
Fig. 6 is light beam supporting structure schematic diagram of the present invention.
In figure, 1, photoreactor tank body; 2, vision slit; 3, upper cover; 4, upper cover; 5, opening for feed; 6, sensor interface; 7, air outlet; 8, discharge port; 9, light bar wire; 10, Glass tubing sealing; 11, Glass tubing; 12, LED; 13, light bar; 14, micro-porous sparger; 15, anchor; 16, take over; 17, power lead; 18, photovoltaic controller; 19, lamppost wire; 20, light beam support; 21, snakelike heating coil; 22, aerosol whipped-up structure; 23, annular cover plate; 24, central manhole; 25, light beam penetrates hole; 26, cross frame; 27, ring support; 28, light beam.
Embodiment:
See shown in Fig. 1 to Fig. 6, the present invention is provided with photoreactor tank body 1, the profile of photoreactor tank body 1 is upright cylindrical cylindrical shell, cylindrical tube diameter is 1:2.5 with the ratio of height, cylindrical tube is provided with opening for feed 5, discharge port 8, central authorities' manhole, vision slit 2, air outlet 7, sensor interface and thief hole, described opening for feed 5 and air outlet 7 are separately positioned on the upper side of cylindrical tube, multiple vision slit 2 is vertical array on cylindrical tube, sensor interface 6 is arranged on the medium position of cylindrical tube, the inside of cylindrical tube is provided with sensor, sensor interface 6 is connected with sensor, sensor comprises some pressure transmitters and some temperature sensors, described pressure transmitter is connected with aerosol whipped-up structure 22 by signal wire with temperature sensor, discharge port 8 is arranged on the bottom position of cylindrical tube, light beam support 20 and aerosol whipped-up structure 22 is also provided with in cylindrical tube inside, described light beam support 20 is horizontally set on the medium position of cylindrical tube, aerosol whipped-up structure 22 is horizontally set on the bottom of cylindrical tube, upper cover 3 is provided with above cylindrical tube, upper cover 4 is provided with above upper cover 3, described light beam support 20 comprises cross frame 26 and ring support 27, ring support 27 is rounded, hole 25 is penetrated at the light beam being circumferentially provided with equidistantly arrangement of ring support 27, described cross frame 26 and ring support 27 weld in one plane, described upper cover 3 is provided with annular cover plate 23 and described cross frame 26, the excircle of annular cover plate 23 is provided with described light beam and penetrates hole 25, the center of annular cover plate 23 is described central manhole 24, annular cover plate 23 and described cross frame 26 weld in one plane, penetrate in hole 25 at described light beam and be installed with light beam 28, the top of described light beam 28 is connected with a power lead 17 by lamppost wire 19, described power lead 17 is connected with a photovoltaic controller 18, described photovoltaic controller 18 is arranged in described central manhole 24, aerosol whipped-up structure 22 comprises system control unit, micro-porous sparger 14, anchor 15 and adapter 16, described micro-porous sparger 14 is looped around on described anchor 15, described adapter 16 is connected with the initial end of micro-porous sparger 14, snakelike heating coil 21 is circumferentially wound with at cylindrical tube, described snakelike heating coil 21 is connected with aerosol whipped-up structure 22, the system control unit of aerosol whipped-up structure 22 can control the heating of snakelike heating coil 21, also can make self to stir, system control unit controls the operation of whole system.
The center of described anchor 15 is provided with a set collar, and at the some scattering supports of circumferentially equidistant welding of set collar, described scattering support is outside scattering centered by the center of circle of set collar, and described scattering support and set collar are in one plane.
Described light beam 28 comprises Glass tubing 11, light bar wire 9 and light bar 13, it is inner that described light bar 13 is arranged on Glass tubing 11, described light bar wire 9 is connected with light bar 13, and described light bar 13 is made up of the vertical arranged in series of LED12 together, and Glass tubing sealing 10 is arranged on the top of Glass tubing 11.The present invention can be operated in sterilization inoculation and micro-algae breeding phase two patterns, and principle of work is:
1, the present invention is for inoculation of sterilizing
1), after photoreactor tank body 1 is sterilized, pump into from opening for feed 5 and cultivate the conventional filtered water of micro-algae, to constant volume, add chemostefilant from opening for feed 5;
2) open aerosol whipped-up structure 22 and stir 5 minutes, after chemostefilant is stirred, shut down and close the tank mouth be all connected with the outside of photoreactor tank body 1, static 12 hours;
3) residual poison scavenging agent is added from opening for feed 5, open air outlet 7 and aerosol whipped-up structure 12, strong aeration 10 minutes, after residual poison material is thoroughly removed or neutralizing inefficacy, the nutrient chemical through high-temperature sterilization is added from opening for feed 5, stir, open the temperature controlling system in aerosol whipped-up structure 22 simultaneously, make nutrient chemical temperature be adjusted to default temperature.
4), after accessing algae kind from opening for feed 5, opening for feed 5 is closed;
5) open aerosol whipped-up structure 12 and go out aperture 7, be adjusted to micro-mist air input with giving vent to anger and reach balance, pressure transmitter makes a little higher than ambient pressure of tank internal gas pressure;
6) open the light source of photovoltaic controller 18, make intensity of illumination be adjusted to default intensity, enter micro-algae breeding phase.
2, the present invention is used for micro-algae breeding phase, and micro-algae cultivation period is longer, and breeding phase enters adjustment to parameters.
1) temperature regulates: due to day and night temperature, Changes in weather, and usable temp regulation system regulates;
2) due to the growth and breeding of micro-algae, frond increases, and based on the interception of frond, can finely tune increase intensity of illumination;
3) in culturing process because photosynthesis causes the consumption of carbonic acid gas, carbonic acid gas can be increased from aerosol whipped-up structure;
4) change of PH can regulate with soda acid tank;
5) nutraceutical consumption can by the agent of photoreactor tank body 1 Ensure Liquid;
6) optimal culture conditions that micro-algae is cultivated can be adjusted in a word, when Haematocoocus Pluvialls is cultivated, early stage at vegetative cell logarithmic phase, add intense light irradiation, when increasing temperature and feed supplement, regulate nutrient chemical formula, for Haematocoocus Pluvialls increases the accumulation of astaxanthin;
7) when frond grows to default biomass or active substance reaches default amount, open discharge port 5 discharging, cultivating process terminates.
Claims (3)
1. a new bio proofing unit, it is characterized in that: be provided with photoreactor tank body, the profile of photoreactor tank body is upright cylindrical cylindrical shell, cylindrical tube diameter is 1:2.5 with the ratio of height, cylindrical tube is provided with opening for feed, discharge port, central authorities' manhole, vision slit, air outlet, sensor interface and thief hole, described opening for feed and air outlet are separately positioned on the upper side of cylindrical tube, vision slit is vertical array on cylindrical tube, sensor interface is arranged on the medium position of cylindrical tube, the inside of cylindrical tube is provided with sensor, sensor interface is connected with sensor, sensor comprises pressure transmitter and temperature sensor, described pressure transmitter is connected with aerosol whipped-up structure by signal wire with temperature sensor, discharge port is arranged on the bottom position of cylindrical tube, light beam support and aerosol whipped-up structure is provided with in cylindrical tube inside, described light beam support level is arranged on the medium position of cylindrical tube, aerosol whipped-up structure is horizontally set on the bottom of cylindrical tube, upper cover is provided with above cylindrical tube, upper cover is provided with above upper cover, described light beam support comprises cross frame and ring support, ring support is rounded, hole is penetrated at the light beam being circumferentially provided with equidistantly arrangement of ring support, described cross frame and ring support welding are in one plane, described upper cover is provided with annular cover plate and described cross frame, the excircle of annular cover plate is provided with described light beam and penetrates hole, the center of annular cover plate is described central manhole, annular cover plate and the welding of described cross frame are in one plane, penetrate in hole at described light beam and be installed with light beam, the top of described light beam is connected with a power lead by lamppost wire, described power lead is connected with a photovoltaic controller, described photovoltaic controller is arranged in described central manhole, aerosol whipped-up structure comprises system control unit, micro-porous sparger, anchor and adapter, described micro-porous sparger is looped around on described anchor, described adapter is connected with the initial end of micro-porous sparger, snakelike heating coil is circumferentially wound with at cylindrical tube, described snakelike heating coil is connected with aerosol whipped-up structure.
2. new bio proofing unit according to claim 1, it is characterized in that: the center of described anchor is provided with a set collar, at the some scattering supports of circumferentially equidistant welding of set collar, described scattering support is outside scattering centered by the center of circle of set collar, and described scattering support and set collar are in one plane.
3. new bio proofing unit according to claim 1 and 2, it is characterized in that: described light beam comprises Glass tubing, light bar wire and light bar, described light bar is arranged on glass tube, and described light bar wire is connected with light bar, and described light bar is made up of the vertical arranged in series of LED together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410402665.5A CN105331530A (en) | 2014-08-17 | 2014-08-17 | Novel biological detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410402665.5A CN105331530A (en) | 2014-08-17 | 2014-08-17 | Novel biological detection device |
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| CN105331530A true CN105331530A (en) | 2016-02-17 |
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| CN201410402665.5A Pending CN105331530A (en) | 2014-08-17 | 2014-08-17 | Novel biological detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244426A (en) * | 2016-08-27 | 2016-12-21 | 江苏翔康海洋生物科技有限公司 | High density Large-scale microalgae culture apparatus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709262A (en) * | 2009-12-10 | 2010-05-19 | 中国科学院广州能源研究所 | Solar spectral photosynthetic bioreactor system for culturing microalgae in high density |
| CN102634443A (en) * | 2012-05-08 | 2012-08-15 | 青岛中仁药业有限公司 | Abnormal fermenting device for microalgae |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
| CN203159616U (en) * | 2013-04-22 | 2013-08-28 | 青岛中仁药业有限公司 | Mist type microalgae illumination reaction device |
-
2014
- 2014-08-17 CN CN201410402665.5A patent/CN105331530A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709262A (en) * | 2009-12-10 | 2010-05-19 | 中国科学院广州能源研究所 | Solar spectral photosynthetic bioreactor system for culturing microalgae in high density |
| CN102634443A (en) * | 2012-05-08 | 2012-08-15 | 青岛中仁药业有限公司 | Abnormal fermenting device for microalgae |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
| CN203159616U (en) * | 2013-04-22 | 2013-08-28 | 青岛中仁药业有限公司 | Mist type microalgae illumination reaction device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244426A (en) * | 2016-08-27 | 2016-12-21 | 江苏翔康海洋生物科技有限公司 | High density Large-scale microalgae culture apparatus |
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Application publication date: 20160217 |