CN106173791A - A kind of pulse supertension sterilize method of liquid food - Google Patents
A kind of pulse supertension sterilize method of liquid food Download PDFInfo
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- CN106173791A CN106173791A CN201610579820.XA CN201610579820A CN106173791A CN 106173791 A CN106173791 A CN 106173791A CN 201610579820 A CN201610579820 A CN 201610579820A CN 106173791 A CN106173791 A CN 106173791A
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- 238000000034 method Methods 0.000 title claims abstract description 94
- 235000021056 liquid food Nutrition 0.000 title claims abstract description 21
- 230000008569 process Effects 0.000 claims abstract description 54
- 230000001954 sterilising effect Effects 0.000 claims abstract description 44
- 238000011282 treatment Methods 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 235000013305 food Nutrition 0.000 claims abstract description 30
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 29
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 25
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 244000005700 microbiome Species 0.000 claims abstract description 15
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 8
- 239000004310 lactic acid Substances 0.000 claims abstract description 8
- 229920001661 Chitosan Polymers 0.000 claims abstract description 7
- 108010039918 Polylysine Proteins 0.000 claims abstract description 6
- 229920000656 polylysine Polymers 0.000 claims abstract description 6
- 239000012535 impurity Substances 0.000 claims abstract description 3
- 239000007787 solid Substances 0.000 claims abstract description 3
- 238000007599 discharging Methods 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 17
- 238000003825 pressing Methods 0.000 claims description 15
- 239000000295 fuel oil Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 10
- 230000033001 locomotion Effects 0.000 claims description 8
- 230000007306 turnover Effects 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 5
- 230000000630 rising effect Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 230000035485 pulse pressure Effects 0.000 claims description 2
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- 230000008676 import Effects 0.000 claims 1
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- 239000000796 flavoring agent Substances 0.000 abstract description 5
- 235000019634 flavors Nutrition 0.000 abstract description 5
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- 230000035764 nutrition Effects 0.000 abstract description 3
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- 230000000694 effects Effects 0.000 description 29
- 238000013461 design Methods 0.000 description 21
- 238000012545 processing Methods 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 12
- 235000013405 beer Nutrition 0.000 description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 7
- 241000251468 Actinopterygii Species 0.000 description 6
- 239000008267 milk Substances 0.000 description 6
- 210000004080 milk Anatomy 0.000 description 6
- 235000013336 milk Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 208000033999 Device damage Diseases 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 241000186779 Listeria monocytogenes Species 0.000 description 3
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- 230000003385 bacteriostatic effect Effects 0.000 description 3
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- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
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- 239000003242 anti bacterial agent Substances 0.000 description 1
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- 230000001580 bacterial effect Effects 0.000 description 1
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- 230000003139 buffering effect Effects 0.000 description 1
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- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/015—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation
- A23L3/0155—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The present invention relates to a kind of pasteurisation method, particularly to a kind of pulse supertension sterilize method of liquid food.A kind of pulse supertension sterilize method of liquid food, method comprises the steps: 1) select fresh liquid food, carry out pretreatment, remove the solid impurity that may carry microorganism；2) liquid food is added appropriate antibacterial, and be pre-chilled to less than 10 DEG C；3) pressurizing chamber of liquid food input pulse superhigh pressure liquid apparatus for continuous treatment carries out pulse ultra high pressure treatment.One or more in lactic acid, chitosan, polylysine of antibacterial, antibacterial concentration in the mixed liquor of liquid food is not less than 0.1% mass fraction.The method that the present invention provides is non-thermal sterilization method, can the color and luster local flavor matter structure of retaining food product and nutrition to greatest extent, simultaneously because pulsating pressure processes the most shorter, the time that processes processes than independent static pressure and saves more than 2/3.
Description
Technical field
The present invention relates to a kind of pasteurisation method, particularly to a kind of pulse supertension sterilize method of liquid food.
Background technology
Supertension sterilize, as a kind of emerging thermal Sterilization Technologies in Food Processing, because it is keeping the original local flavor of food, improves
Food Texture, and avoid the advantage in terms of the thermo-responsive nutrient substance losses such as vitamin, favored by food industry.But should
Technology still suffers from some defects, does not thoroughly threaten food sanitation safe as relatively low processing pressure is easily caused sterilizing, and higher position is managed
Pressure is then easily caused the shortcoming that processing equipment cost is too high and processing cost is high, and these shortcomings limit supertension sterilize skill
Art is in the application of food industry.The most conventional supertension sterilize technology, its pressure sterilization process high static pressure of many uses processes, enters
And make the microorganism in food and enzyme inactivation, thus play the effect of sterilizing.And static pressure processes and need to reach preferable sterilizing
Effect needs the longer process time, and this is unfavorable for the use frequency of raising equipment, according to correlational study, uses the quick ripple of pulse
Dynamic pressure processes and can play higher sterilization effect within the shorter time, and then improves the utilization rate of equipment, reduces sterilizing
The production cost of process.
Antibacterial, as the basis of guarantee industrialized production food hygiene, plays very important work in field of food industry
With, but along with the development of society, food safety is more paid attention to by people, and owing to Social Media is the most proper to synthetic antibacterial
When demonization publicity and part wicked business abuse synthetic antibacterial so that the synthetic in food is pressed down by consumer
Fungus matter produces psychology of conflicting.If more winning for this antibacterial substance having some natural bacteriostatic materials to substitute chemosynthesis and disappearing
The person of expense favors.The mechanism of action of different antibacterial is different, has plenty of destruction microorganism wall, has plenty of and cause microprotein
Matter, enzyme inactivate, and significantly reduce the resistance of antibacterial according to the microorganism of early-stage Study result, cell wall or cell membrane damage,
And the main mechanism of action of pulse ultra high pressure treatment is i.e. to destroy bacteria cell wall or the integrity of cell membrane, both combine can
To significantly improve sterilization effect so that being issued to that good sterilization effect becomes in relatively low processing pressure and relatively low Mlc can
Energy.
Fresh-keeping problem at the various food of food service industry is one of bottleneck of puzzlement industry development, the most general thermal sterilization
Technique all has many defects in terms of mouthfeel nutrient substance heat time heating time preservation, and these defects badly influence the commodity valency of food
Value, also has influence on the economic benefit of food enterprise.The innovation processing equipment that the present invention provides can realize food under the conditions of non-thermal
The quick sterilization of product, the element such as local flavor sense organ nutrition ensureing food on the premise of guaranteeing food safety to greatest extent, can
To significantly improve the commodity value of food, the design simultaneously innovated can realize supertension on the premise of relatively low processing cost and go out
Bacterium, the popularization for superhigh pressure technique has bigger value.The natural bacteriostatic product of relatively low processing pressure and low concentration
Can produce good collaborative sterilization effect under certain condition, the popularization of this technology can promote that superhigh pressure technique adds at food
The application in work field, can reduce the application in food of the synthetic antibacterial simultaneously, have bigger social value and warp
Ji is worth.
Summary of the invention
The present invention provides a kind of pulse supertension sterilize method of liquid food, and the method utilizes pulse supertension to coordinate to press down
Food is processed by microbial inoculum, it is achieved that quickly pasteurisation.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of pulse supertension sterilize method of liquid food, method comprises the steps:
1) select fresh liquid food, carry out pretreatment, remove the solid impurity that may carry microorganism；
2) liquid food is added appropriate antibacterial, and be pre-chilled to less than 10 DEG C；
3) pressurizing chamber of liquid food input pulse superhigh pressure liquid apparatus for continuous treatment carries out pulse ultra high pressure treatment.
As preferably, one or more in lactic acid, chitosan, polylysine of antibacterial, antibacterial is eaten at liquid
Concentration in the mixed liquor of product is not less than 0.1% mass fraction.
As preferably, the parameter of pulse ultra high pressure treatment is that surge pressure is not less than 250Mpa, when boosting in pressurizing chamber
Between be not more than 2s, be not more than 2s in dip time, the single dwell time is not shorter than 2s, and sterilization process is minimum carries out 10 arteries and veins
Stamping press processes, and the single pulse pressure intervals time is less than 2s.
The sterilizing methods of the present invention based on the pulse superhigh pressure liquid apparatus for continuous treatment of a kind of particular design, in conjunction with
The fungistatic effect of part natural bacteriostatic material, destroys microorganism wall by pulsating pressure, causes Most bacterial dead,
And pressure enhances the fungistatic effect of antibacterial to the damage of microorganism, work in coordination with antibacterial by pulse supertension and realize liquid
The commercial sterilization of food.The present invention can kill pathogenic microorganism trophosome main in food and suppress the increasing of microbial spores
Grow, the food unimpeded system taste after sterilization treatment, substantially remain original local flavor and the matter structure feature of food.
As preferably, described pulse superhigh pressure liquid apparatus for continuous treatment, including instantaneous pressure generator, impulsive force
Buffer unit, liquid pressing process chamber device, valve control device and chiller；Described instantaneous pressure generator is by least
One fuel oil piling machine composition；Impulsive force buffer unit includes reducing piston, yielding rubber pad, spring and cylinder；Reducing piston
It is positioned at fuel oil piling machine lower end；The end, heavy in section of reducing piston is connected with the pressure hammer of fuel oil piling machine；Reducing piston
Small bore end sleeve is connected to spring, and spring is positioned at cylinder；Bottom of cylinder, the other end and reducing piston are fixed in one end of spring
Offset；Yielding rubber pad is between barrel top and reducing piston；Cylinder is fixed on liquid pressing and processes on the device of chamber；Liquid
Pressurized treatments chamber device controls material by valve control device and passes in and out.
As preferably, spring housing is in the thin diameter end of reducing piston, and one end is fixed in cylinder, and cylinder upper end is lined with properly
The yielding rubber pad of internal diameter, cylinder is fixed on pressurizing chamber outer upper end, and pressure acts on reducing piston, anti-at the elastic force of spring
Under active force, it is descending that piston presses certain speed, until encountering yielding rubber pad, reducing piston movement stops, at the elastic force of spring
Under effect up.
As preferably, liquid pressing processes cavity outer wall and is provided with chiller；Described liquid pressing processes chamber device and includes
Pressurizing chamber and at least three sealing ring, sealing ring is arranged on reducing piston small bore end outermost end position；Pressurizing chamber one end connects
Reducing piston, slipper seal；The other end is semi-closed structure, leaves two discharging needle-valves controlling material turnover and charging pin
Valve；Cylinder is fixed on the outer upper end of pressurizing chamber.
As preferably, described sealing ring interval is installed, and the spacing between adjacent seals ring is identical.
As preferably, described valve control device include guide rod, connecting rod, flywheel, ball, big cam, little cam, shadoof,
Discharging needle-valve and charging needle-valve；Guide rod is fixedly connected on the left and right end of reducing piston, parallel with reducing piston movement direction, leads
Bar moves with under reducing piston in the vertical direction；The lower end of the guide rod of the left and right sides is connected with connecting rod and flywheel in turn；Left and right two
The most coaxial big cam and the little cam of being provided with of the flywheel of side, and flywheel drives big cam and little cam to rotate, and shadoof is close
Big cam and little cam position are provided with ball；Big cam and little cam drive shadoof respectively against the ball being arranged on shadoof
Move up and down, for controlling discharging needle-valve and the opening and closing of charging needle-valve.
As preferably, described big cam rotates 0 °～180 ° counterclockwise, and charging needle-valve opened by big cam jack-up shadoof,
Rotating to 180 °～360 °, shadoof falls after rise closes charging needle-valve.
As preferably, described big cam is made up of big protuberance and orthodrome portion；The arc radius of big lug position is big
In more than the 25% of the arc radius of position, orthodrome portion, and the circular arc of big protuberance accounts for the half of overall big cam arc angle.
As preferably, described little cam is made up of little protuberance and small arc-shaped portion；The arc radius of little lug position is big
Arc radius 15% in position, small arc-shaped portion.And the circular arc of little lug position accounts for the 7/12 of overall small cam circular arc angle, i.e.
210 °, and the circular arc of position, small arc-shaped portion accounts for the 5/12 of overall small cam circular arc angle, i.e. 150 °.
As preferably, described chiller includes the condensing tube being wound in pressurizing chamber outer wall.
This device is originated using piling machine as pulse shock power, has technology maturation, with low cost, easy to use etc. excellent
Gesture；Add spring by unique reducing piston and add damping gasket design, if the instant impact produced by piling machine hammer amplifies
Dry times, and the collision of reducing piston and process chambers is avoided by spring and damping washer design, it is to avoid device damages, simultaneously
Due to the existence of spring, i.e. can extend pressure rise time and avoid boosting and too fast cause the device damage again can be by spring
Elastic force promotes reducing piston stroking upward, prepares to pressurize next time, and then promotes being carried out continuously of pulse ultra high pressure treatment.
The crankshaft-link rod of this device unique design combines cam and needle-valve design, can well control pending liquid and enter
Go out to process chamber to be connected with pulse ultra high pressure treatment process, by guide rod flywheel and connecting rod, the linear motion of reducing piston is changed
Feed needle-valve and the opening and closing of discharging needle-valve for the rotary motion of cam, cam driven shadoof and then control, thus realize
The Automatic continuous turnover sample of device.
Two size cams of this device unique design, the entrance of material when big cam is for controlling the rising of reducing piston,
Need normally open in this process, and ensure that bigger unlatching amplitude is conducive to Liquid sample introduction complete, and the little cam of special angle sets
Meter then controls emphatically sample needle-valve and cuts out with pressurization moment so that liquid bears bigger pressure, descending along with reducing piston,
Little cam slightly jack-up push rod, needle-valve is slightly opened so that fluid is imitated with strengthening pressure treatment by needle-valve under bigger pressure
Really.Pressure treatment moment, owing to the rate of rise is very fast, process fluid and easily produce obvious heat effect, and then affect at pressure
Reason effect, the chiller of this device unique design, process cavity temperature to overcome above-mentioned heat effect with cryogen reduction.
This device uses piling machine pulse shock power source, designs and automatic valve control device by slowing down impulsive force,
And heat dissipation design, it is possible at lower cost, produce continuous impulse supertension, all can regulate moulding pressure and pressing time,
There is broader prospect of the application.
The invention has the beneficial effects as follows: the sterilizing methods that the present invention provides is applicable to the liquid food of room temperature storage, can reach
To the killing effect of 6 log difference of Listeria monocytogenes, the killing effect of 6.5 log difference of staphylococcus aureus.Separately
Outward, due to pulsating pressure and the synergism of antibacterial in the inventive method, compare simple pulsating pressure and process or the most antibacterial
Agent processes fungistatic effect more preferably, more than effect 2 log difference bigger than the effect of individual processing of collaborative sterilizing.Compare identical often
Sterilization effect more than rule big 1 log difference of heat treatment.
The method that the present invention provides can be killed most food pathogenic microorganism and suppress the propagation of microorganism, residing
The liquid food of reason has the shelf of more than six months to store the time.The method that the present invention provides is non-thermal sterilization method, can be
The color and luster local flavor matter structure of the retaining food product of limits and nutrition, simultaneously because pulsating pressure processes the most shorter, process time ratio
Individually static pressure processes and saves more than 2/3.
Accompanying drawing explanation
Fig. 1 is the structural representation according to pulse superhigh pressure liquid apparatus for continuous treatment embodiment of the present invention.
Fig. 2 is the use state diagram according to pulse superhigh pressure liquid apparatus for continuous treatment embodiment of the present invention.
Fig. 3 is the impulsive force buffer unit structure schematic diagram according to the present invention.
Fig. 4 is that the liquid pressing according to the present invention processes cavity configuration schematic diagram.
Fig. 5 is the valve control device structural representation according to the present invention.
Fig. 6 is the size cam structure detailed maps according to the present invention
Fig. 7 is the condensing unit structural representation according to the present invention.
Reference in figure: fuel oil piling machine 1, reducing piston 2, yielding rubber pad 3, spring 4, cylinder 5, guide rod 6, pressurization
Chamber 7, sealing ring 8, connecting rod 9, flywheel 10, ball 11, big cam 12, big protuberance 12-1, orthodrome portion 12-2, little cam 13,
Little protuberance 13-1, small arc-shaped portion 13-2, shadoof 14, discharging needle-valve 15, feed needle-valve 16, condensing tube 17.
Detailed description of the invention
Below by specific embodiment, and combine accompanying drawing, technical scheme is described in further detail.Should
Working as understanding, the enforcement of the present invention is not limited to the following examples, any pro forma accommodation that the present invention is done and/or
Change and fall within scope.
In the present invention, if not refering in particular to, all of part, percentage ratio are unit of weight, the equipment used and raw material etc.
All it is commercially available or commonly used in the art.Method in following embodiment, if no special instructions, is the normal of this area
Rule method.
Embodiment 1
The pulse supertension sterilize of Listeria monocytogenes, its step is as follows:
(1) taking Listeria monocytogenes logarithmic (log) phase culture fluid, repeatedly centrifuge washing removes culture medium, resuspended with normal saline.
(2) being diluted with the milk adding lactic acid by bacteria suspension, be diluted to containing 0.1% lactic acid, clump count is 1 × 108CFU/ml
Concentration.
(3) milk containing lactic acid diluted is entered into pressurizing chamber by material inlet valve, by the condensation of pressurizing chamber outer wall
Within Radix Dauci Sativae juice is pre-chilled to 10 DEG C by pipe, bacteria suspension fills full pressurizing chamber and the space of piston.
(4) milk in pressurizing chamber being carried out pulse ultra high pressure treatment, processing parameter is: surge pressure in pressurizing chamber
250Mpa, pressure rising time 2s, dip time 2s, single dwell time 2s, sterilization process carries out 10 subpulse pressure treatment, single
Pulsating pressure 2s interval time.
(5) milk handled well flows out from outlet valve, and sterile packaged takes milk after being placed under room temperature storage 72h and carries out bacterium
Fall counting, compares untreated fish group, and the clump count log difference of these treatment conditions reaches 6, independent lactic acid and independent pressure treatment bacterium colony
Number log difference is 4, and untreated fish group clump count log difference is only 1, and this technique has good sterilization effect.
The present embodiment utilizes pulse supertension to work in coordination with antibacterial sterilizing, and in sterilization process, heat production is few, and sterilizing object heats up few,
The lethality mechanisms of microorganism does not lies in thermodynamic activity, and is that microbial cell film is produced by transient fluctuation pressure and destroys, and then
Make it that resistance of antibacterial substance to be drastically reduced, thus strengthen the ability of antibacterial suppression microbial growth, until microorganism is dead
Die.The sterilizing log difference of the milk after the present embodiment sterilizing reaches 6, is better than 5 log difference of common heat treatment.
Embodiment 2
The pulse supertension sterilize of staphylococcus aureus, its step is as follows:
(1) depletion Staphylococcus aureus logarithmic (log) phase culture fluid, repeatedly centrifuge washing removes culture medium, resuspended with normal saline.
(2) by bacteria suspension with adding the Radix Dauci Sativae juice dilution of lactic acid, it is diluted to containing 0.1% chitosan, clump count is 1 ×
108The concentration of CFU/ml.
(3) the chitosan-containing Radix Dauci Sativae juice diluted is entered into pressurizing chamber by material inlet valve, by pressurizing chamber outer wall
Within bacteria suspension is pre-chilled to 10 DEG C by condensing tube, Radix Dauci Sativae juice fills full pressurizing chamber and the space of piston.
(4) Radix Dauci Sativae juice in pressurizing chamber being carried out pulse ultra high pressure treatment, processing parameter is: surge pressure in pressurizing chamber
250Mpa, pressure rising time 2s, dip time 2s, single dwell time 2s, sterilization process carries out 10 subpulse pressure treatment, single
Pulsating pressure 2s interval time.
(5) Radix Dauci Sativae juice handled well flows out from outlet valve, and sterile packaged takes Radix Dauci Sativae juice after being placed under room temperature storage 72h
Carrying out colony counting, compare untreated fish group, the clump count log difference of these treatment conditions reaches 6.5, independent treatment with chitosan bacterium colony
Number log difference is 3, and independent pressure treatment clump count log difference is 4, and untreated fish group clump count log difference is only 1, should
Technique has good sterilization effect.
The present embodiment utilizes pulse supertension to work in coordination with antibacterial sterilizing, and in sterilization process, heat production is few, and sterilizing object heats up few,
The lethality mechanisms of microorganism does not lies in thermodynamic activity, and is that microbial cell film is produced by transient fluctuation pressure and destroys, and then
Make it that resistance of antibacterial substance to be drastically reduced, thus strengthen the ability of antibacterial suppression microbial growth, until microorganism is dead
Die.The sterilizing log difference of the Radix Dauci Sativae juice after the present embodiment sterilizing reaches 6.5, is better than 5 log difference of common heat treatment
Sterilization effect.
Embodiment 3
Colibacillary pulse supertension sterilize, its step is as follows:
(1) taking escherichia coli logarithmic (log) phase culture fluid, repeatedly centrifuge washing removes culture medium, resuspended with normal saline.
(2) by bacteria suspension with adding the beer dilution of polylysine, it is diluted to containing 0.1% chitosan, clump count is 1 ×
108The concentration of CFU/ml.
(3) medicated beer containing polylysine diluted is entered into pressurizing chamber by material inlet valve, by pressurizing chamber outer wall
Within medicated beer is pre-chilled to 10 DEG C by condensing tube, medicated beer fills full pressurizing chamber and the space of piston.
(4) medicated beer in pressurizing chamber being carried out pulse ultra high pressure treatment, processing parameter is: surge pressure in pressurizing chamber
250Mpa, pressure rising time 2s, dip time 2s, single dwell time 2s, sterilization process carries out 10 subpulse pressure treatment, single
Pulsating pressure 2s interval time.
(5) medicated beer handled well flows out from outlet valve, and sterile packaged takes medicated beer after being placed under room temperature storage 72h and carries out bacterium
Fall counting, compares untreated fish group, and the clump count log difference of these treatment conditions reaches 6.8, and independent polylysine processes clump count pair
Number difference is 3.5, and independent pressure treatment clump count log difference is 4.5, and untreated fish group clump count log difference is only 1.2,
This technique has good sterilization effect.
The present embodiment utilizes pulse supertension to work in coordination with antibacterial sterilizing, and in sterilization process, heat production is few, and sterilizing object heats up few,
The lethality mechanisms of microorganism does not lies in thermodynamic activity, and is that microbial cell film is produced by transient fluctuation pressure and destroys, and then
Make it that resistance of antibacterial substance to be drastically reduced, thus strengthen the ability of antibacterial suppression microbial growth, until microorganism is dead
Die.The sterilizing log difference of the medicated beer after the present embodiment sterilizing reaches 6.8, is better than going out of 5 log difference of common heat treatment
Bacterium effect.
Embodiment: the processing means that the inventive method uses
A kind of pulse superhigh pressure liquid apparatus for continuous treatment, adds including instantaneous pressure generator, impulsive force buffer unit, liquid
Pressure processes chamber device, valve control device and chiller；Instantaneous pressure generator is by least one fuel oil piling machine 1 group
Become；Fuel oil piling machine 1 is arranged on the top of pulse superhigh pressure liquid apparatus for continuous treatment, and its work produces instant impact,
As instantaneous pressure generator, pressure amplifies by connecting the impulsive force buffer unit of fuel oil piling machine 1 and pressurizing chamber 7 and delays
Punching, impact force action processes on the liquid of intracavity in liquid pressing, is arranged on the valve on the input and output material pipeline connecting pressurizing chamber 7
Controlling device and automatically control material turnover realization work continuously, and chiller is arranged on and adds pressure outer wall, it is overheated to place to prevent
The impact of reason liquid；Originate as pulse shock power using fuel oil piling machine 1, there is technology maturation, with low cost, easy to use etc.
Advantage.
As it is shown on figure 3, impulsive force buffer unit includes reducing piston 2, yielding rubber pad 3, spring 4 and cylinder 5；Reducing is lived
Plug 2 is positioned at fuel oil piling machine 1 lower end；The end, heavy in section of reducing piston 2 is connected with the pressure hammer of fuel oil piling machine 1；Reducing
Piston 2 small bore end passes spring 3, is deep into pressurizing chamber 7 upper end, and spring 4 is positioned at cylinder 5；Circle is fixed in one end of spring 4
Bottom cylinder 5, the other end offsets with reducing piston 2；Yielding rubber pad 3 is between cylinder 5 top and reducing piston 2；Work as reducing
When piston 2 vertical direction moves spring serve buffering and pressurization complete after promote reducing piston 2 to return to the front initial position that pressurizes
Effect.Pad yielding rubber pad between barrel top and reducing piston moves to least significant end at reducing piston, touches with cylinder 5
Playing cushioning effect when hitting, anti-locking apparatus damages；Cylinder 5 is fixed on liquid pressing and processes on the device of chamber；Liquid pressing processes chamber
Device controls material by valve control device and passes in and out.
By unique reducing piston 2, spring 4 and the design of yielding rubber pad 3, fuel oil piling machine 1 hammer is produced
Instant impact amplifies several times, and avoids reducing piston 2 and process chambers by spring 4 and yielding rubber pad 3 design
Collision, it is to avoid device damages, simultaneously because the existence of spring 4, i.e. can extend pressure rise time and avoid too fast causing of boosting
Device damage can promote reducing piston 2 up by the elastic force of spring 4 again, prepares to pressurize next time, and then promotes pulse to surpass
Being carried out continuously of HIGH PRESSURE TREATMENT.
As shown in Figure 4, described liquid pressing process chamber device includes pressurizing chamber 7 and at least three sealing ring 8, sealing ring 8
It is arranged on reducing piston 2 small bore end outermost end position；Sealing ring 8 interval is installed, and the spacing between adjacent seals ring 8 is identical；
When reducing piston 2 moves up and down, the effect of sealing ring 8 ensures the close of pressurizing chamber 7 on the premise of being to ensure that certain lubricant effect
Feng Xing, prevents pressure process liquid from overflowing.Pressurizing chamber 7 one end connects reducing piston 2, slipper seal；The other end is semiclosed knot
Structure, leaves two discharging needle-valves 15 controlling material turnover and charging needle-valve 16；Cylinder 5 is fixed on the outer upper end of pressurizing chamber 7.
As it is shown in figure 5, be valve control device arrangement works example, under pressure effect, reducing piston 2 vertically drives
Guide rod 6 moves downward, and connecting rod 9 one end is flexibly connected with guide rod 6, and the other end is flexibly connected with flywheel 10, and this design can will be led
The transform linear motion of bar 6 is the rotary motion of flywheel 10；Flywheel 10 coaxial installation big cam 12 and little cam 13, shadoof respectively
14 in inverted " L " shape, and shadoof 14 upper end is provided with ball 11 near the position of big cam 12 and little cam 13, to reduce shadoof 14
And the frictional force between big cam 12 and little cam 13, contacts position when flywheel 10 drives big cam 12 to move to salient point with ball 11
When putting, shadoof 14 moves upward and moves on charging needle-valve 16 spool that band is dynamically connected, and then opens charging needle-valve 16, when big cam 12
When moving to non-salient point and ball 11 contact position, shadoof 14 fall after rise downwards charging needle-valve 16 spool that band is dynamically connected move down and then
Close charging needle-valve 16；In like manner: when flywheel 10 drives little cam 13 to rotate, ball 11 touches little cam 13 salient position
Time, shadoof 14 moves upward and moves on discharging needle-valve 15 spool that band is dynamically connected, and then opens discharging needle-valve 15, and when ball 11 connects
When contacting little cam 13 non-protruding position, shadoof 14 falls downwards discharging needle-valve 15 spool that band is dynamically connected after rise and moves down, and then closes
Discharging needle-valve 15.
The crankshaft-link rod 9 of unique design of the present invention combines big cam 12, little cam 13, discharging needle-valve 15 and charging needle-valve 16
Design, can well control pending liquid turnover process chamber and be connected with pulse ultra high pressure treatment process, by guide rod 6, fly
Annexation between wheel 10 and connecting rod 9, is converted to big cam 12 and the rotation of little cam 13 by the linear motion of reducing piston 2
Moving, big cam 12 and little cam 13 drive shadoof 14 and then control charging needle-valve 16 and the opening and closing of discharging needle-valve 15,
Thus realize the Automatic continuous turnover sample of device.
As shown in Figure 6, sized by cam structure detailed maps, Fig. 6 left side is big cam 12 structural representation, big cam
12 are made up of big protuberance 12-1 and orthodrome portion 12-2；The arc radius of big protuberance 12-1 position is more than orthodrome portion 12-2
More than the 25% of the arc radius of position, and the circular arc of big protuberance 12-1 accounts for the half of overall big cam 12 arc angle.From just
Beginning inverse position hour hands rotate, and the arc angle of big protuberance 12-1 position is 0 ° to 180 °, the circular arc of 12-2 position, orthodrome portion
Angle is 180 ° to 360 °；This design can ensure that whole big cam 12 starts rotary course counterclockwise from initial position, rotation
During turning 0 ° to 180 °, big cam 12 jack-up shadoof 14 opens charging needle-valve 16, during rotating 180 ° to 360 °, push rod 14
Fall after rise and close charging needle-valve 16.The arc radius of the big protuberance 12-1 position of big cam 12 is more than non-salient position more than 25%
Left and right, it is ensured that the height that big cam 12 forwards big protuberance 12-1 position jack-up shadoof 14 to is higher, open by a larger margin into
Material needle-valve 16, meets the requirement of quick feeding.
In like manner, being little cam 13 structural representation on the right of Fig. 6, little cam 13 is by little protuberance 13-1 and small arc-shaped portion 13-2
Composition；The arc radius of little protuberance 13-1 position is more than the arc radius 15% of 13-2 position, small arc-shaped portion.And little protuberance
The circular arc of 13-1 position accounts for the 7/12 of overall little cam 13 arc angle, i.e. 210 °, and the circular arc of 13-2 position, small arc-shaped portion accounts for
The 5/12 of overall little cam 13 arc angle, i.e. 150 °.
Small arc-shaped portion 13-2 position angle is respectively and rotates 0 ° to 30 ° and 180 ° to 360 ° counterclockwise from initial position.Should
Design can ensure that in whole little cam 13 rotary course counterclockwise, starts to rotate 0 ° to 30 ° ball counterclockwise from initial position
The 11 contact non-projections of little cam, shadoof 14 moves down, and closes discharging needle-valve 15, it is ensured that maintain high pressure conditions in pressurizing chamber 7.From
During 30 ° to 180 °, ball 11 comes into contact with the little protuberance 13-1 of little cam 13, just starts to drive and moves on shadoof 14, opens
Discharging needle-valve 15.Little cam 13 rotates 180 ° to 390 ° process balls 11 counterclockwise and contacts the small arc-shaped portion 13-2 of little cam 13,
Shadoof 14 moves down, and closes discharging needle-valve 15.The arc radius of the little protuberance 13-1 position of little cam 13 is merely greater than small arc-shaped portion
The 15% of 13-2 position arc radius, this design can ensure that little cam 13 rotary course be relayed to little protuberance 13-1 position with
When ball 11 contacts, shadoof 14 rises lower height, and it is less that discharging needle-valve 15 opens amplitude, reduces the material rate of outflow, it is ensured that add
A suitable pressure is maintained in pressure chamber 7.
Big cam 12 and the salient position of little cam 13 and do not protrude radius scale and can adjust according to the actual requirements, the most only
Give one example；Little cam 13 raised zones and non-raised zones angular divisions suitably increase also according to being actually needed or reduce,
The most only with an angle as example.
The big cam 12 of unique design of the present invention and little cam 13, big cam 12 is used for controlling thing when reducing piston 2 rises
The entrance of material, needs normally open in this process, and ensures that bigger unlatching amplitude is conducive to Liquid sample introduction complete, and special angle
Little cam 13 designs, and controls discharging needle-valve 15 with pressurization moment emphatically and closes so that liquid bears bigger pressure, along with change
Footpath piston 2 descending, little cam 13 slightly jack-up shadoof 14, discharging needle-valve 15 is slightly opened so that fluid is under bigger pressure
By needle-valve to strengthen pressure treatment effect.Pressure treatment moment, owing to the rate of rise is very fast, process fluid and easily produce substantially
Heat effect, and then affect pressure treatment effect, the chiller of this device unique design, process chamber temperature with cryogen reduction
Degree is to overcome above-mentioned heat effect.
As it is shown in fig. 7, work example for condensing unit, condensing tube 17 is wrapped in pressurizing chamber 7 outer wall, during device work, and condensation
Liquid flows in condensing tube 17, takes away the heat that pressurization intracavity liquid is produced by press processes.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (9)
1. the pulse supertension sterilize method of a liquid food, it is characterised in that the method comprises the steps:
1) select fresh liquid food, carry out pretreatment, remove the solid impurity that may carry microorganism；
2) liquid food is added appropriate antibacterial, and be pre-chilled to less than 10 DEG C；
3) pressurizing chamber of liquid food input pulse superhigh pressure liquid apparatus for continuous treatment carries out pulse ultra high pressure treatment.
Pulse supertension sterilize method the most according to claim 1, it is characterised in that: antibacterial selected from lactic acid, chitosan,
One or more in polylysine, antibacterial concentration in the antibacterial mixed liquor with liquid food is not less than 0.1% mass
Mark.
Pulse supertension sterilize method the most according to claim 1, it is characterised in that: the parameter of pulse ultra high pressure treatment is
In pressurizing chamber, surge pressure is not less than 250Mpa, and pressure rising time less than 2s, in dip time less than 2s, the single dwell time is not
Being shorter than 2s, the minimum 10 subpulse pressure treatment that carry out of sterilization process, the single pulse pressure intervals time is less than 2s.
Pulse supertension sterilize method the most according to claim 1, it is characterised in that: the method uses pulse superelevation hydraulic fluid
Body apparatus for continuous treatment is carried out, and this device includes that instantaneous pressure generator, impulsive force buffer unit, liquid pressing process chamber dress
Put, valve control device and chiller；Described instantaneous pressure generator is made up of at least one fuel oil piling machine (1)；Punching
The power buffer unit of hitting includes reducing piston (2), yielding rubber pad (3), spring (4) and cylinder (5)；Reducing piston (2) is positioned at combustion
Oil piling machine (1) lower end；The end, heavy in section of reducing piston (2) is connected with the pressure hammer of fuel oil piling machine (1)；Spring (4)
One end be fixed on cylinder (5) bottom, the other end offsets with reducing piston (2)；Yielding rubber pad (3) is positioned at cylinder (5) top
And between reducing piston (2)；Cylinder (5) is fixed on liquid pressing and processes on the device of chamber；Liquid pressing processes chamber device and passes through valve
Controlling device for doors controls material turnover.
Pulse supertension sterilize method the most according to claim 4, it is characterised in that: liquid pressing processes cavity outer wall and installs
There is chiller；Described liquid pressing processes chamber device and includes pressurizing chamber (7) and at least three sealing ring (8), and sealing ring (8) is pacified
It is contained in reducing piston (2) small bore end outermost end position；Pressurizing chamber (7) one end connects reducing piston (2), slipper seal；Another
End is semi-closed structure, leaves two discharging needle-valves (15) controlling material turnover and charging needle-valve (16)；Cylinder (5) is fixed on
The outer upper end of pressurizing chamber (7).
Pulse supertension sterilize method the most according to claim 4, it is characterised in that: packaged food is put into pulse and is surpassed
The pressurizing chamber of high-pressure treatment apparatus, the volume adding food is less than the 80% of pressurizing chamber volume, opens inlet valve and will be cooled to 10
DEG C pressure medium imports pressurizing chamber, fills up the space between pressurizing chamber and reducing piston.
Pulse supertension sterilize method the most according to claim 4, it is characterised in that: described valve control device includes leading
Bar (6), connecting rod (9), flywheel (10), ball (11), big cam (12), little cam (13), shadoof (14), discharging needle-valve (15) and
Charging needle-valve (16)；Guide rod (6) is fixedly connected on the left and right end of reducing piston (2), parallel with reducing piston (2) direction of motion,
Guide rod (6) moves with under reducing piston (2) in the vertical direction；Guide rod (6) lower end of the left and right sides is connected with connecting rod (9) in turn
With flywheel (10)；The most coaxial big cam (12) and the little cam (13) of being provided with of the flywheel (10) of the left and right sides, and flywheel
(10) driving big cam (12) and little cam (13) to rotate, shadoof (14) is installed near big cam (12) and little cam (13) position
There is ball (11)；Big cam (12) and little cam (13) drive shadoof respectively against the ball (11) being arranged on shadoof (12)
(12) move up and down, be used for controlling discharging needle-valve (15) and the opening and closing of charging needle-valve (16).
Pulse supertension sterilize method the most according to claim 4, it is characterised in that: described big cam (12) revolves counterclockwise
Rotating 0 °～180 °, charging needle-valve (16) opened by big cam (12) jack-up shadoof (14), rotates to 180 °～360 °, shadoof
(14) closedown charging needle-valve (16) is fallen after rise.
Pulse supertension sterilize method the most according to claim 4, it is characterised in that: described big cam (12) is by protruding greatly
Portion (12-1) and orthodrome portion (12-2) composition；The arc radius of big protuberance (12-1) position is more than orthodrome portion (12-2) position
More than the 25% of the arc radius put, and the circular arc of big protuberance (12-1) accounts for the half of overall big cam (12) arc angle.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107593867A (en) * | 2017-10-27 | 2018-01-19 | 宁波星箭航天机械有限公司 | Fresh shrimp shellfish intelligence extrusion machining equipment |
| CN109259037A (en) * | 2018-10-25 | 2019-01-25 | 漳州明建机械设备制造有限公司 | A kind of continuous impulse formula ultra-high pressure apparatus of liquid food |
| CN113016876A (en) * | 2019-12-24 | 2021-06-25 | 内蒙古蒙牛乳业（集团）股份有限公司 | Process for improving ultrahigh pressure sterilization effect and fresh milk |
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| CN1712071A (en) * | 2004-06-21 | 2005-12-28 | 王盛民 | Superhigh pressure water jetting sterilizing method |
| CN102283418A (en) * | 2011-06-03 | 2011-12-21 | 张守勤 | Ultrahigh-cold isostatic pressing continuous sterilization method and device for liquid food |
| CN203279802U (en) * | 2013-06-13 | 2013-11-13 | 王虎峰 | Firing pulse ultrahigh-pressure food processing device |
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| CN1712071A (en) * | 2004-06-21 | 2005-12-28 | 王盛民 | Superhigh pressure water jetting sterilizing method |
| CN1653917A (en) * | 2005-01-05 | 2005-08-17 | 天津市华泰森淼生物工程技术有限公司 | Milk sterilizing method under hypothermia and super pressure |
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| CN107593867A (en) * | 2017-10-27 | 2018-01-19 | 宁波星箭航天机械有限公司 | Fresh shrimp shellfish intelligence extrusion machining equipment |
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| CN113016876A (en) * | 2019-12-24 | 2021-06-25 | 内蒙古蒙牛乳业（集团）股份有限公司 | Process for improving ultrahigh pressure sterilization effect and fresh milk |
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