CN106173237A - A kind of laying hen Chinese medicine compound probiotic biological preparation and preparation method thereof - Google Patents
A kind of laying hen Chinese medicine compound probiotic biological preparation and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of laying hen Chinese medicine compound probiotic biological preparation and preparation method thereof, composite probiotics ferment Chinese medicine mixed powder prepare；Chinese medicine mixed powder ratio by weight is prepared from by the Radix Astragali 10 20 parts, Herb Gynostemmae Pentaphylli 10 20 parts, Radix Isatidis 20 30 parts, Radix Angelicae Sinensis 58 parts, the Rhizoma Atractylodis Macrocephalae 58 parts, Rhizoma Chuanxiong 58 parts, Radix Dichroae 10 15 parts, Herba Agrimoniae 10 15 parts, the Folium Ligustri Lucidi 20 30 parts, Herba Pouzolziae Zeylanicae 58 parts, Concretio Silicea Bambusae 58 parts, Semen Vaccariae 79 parts, the Radix Pulsatillae 58 parts, Folium Cinnamomi porrecti 10 20 parts；Probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed bacterial of cereuisiae fermentum composition.The laying hen of present invention Chinese medicine compound probiotic biological preparation, matches Chinese medicine with compound probiotic, can strengthen gastrointestinal function and the ovarian function of laying hen, improves autoimmunity, promotes the growth of chickling, reduces sickness rate, improves the egg production of laying hen.
Description
Technical field
The present invention relates to veterinary drug technical field, particularly relate to a kind of laying hen Chinese medicine compound probiotic biological preparation and system thereof
Preparation Method.
Background technology
Along with the intensive culture of laying hen, for improving culture benefit, promoting the growth of laying hen and improve egg production, many anti-
Raw element, chemical synthetic drug, steroid hormone etc. are commonly used, and the too much or nutrition of concentrate is unbalanced in addition, causes chickling
Retarded growth, hepatorenal damage, though egg production increases, but eggshell is thin, frangible and abnormal-shape egg too much, the color, smell and taste of egg and product
Matter declines, and has the drug residue of antibiotic and hormone.The biggest loss is caused to breeding layer chicken industry.
Summary of the invention
The invention aims to overcome the deficiencies in the prior art, it is provided that a kind of laying hen is biological with Chinese medicine compound probiotic
Preparation, matches Chinese medicine with compound probiotic, can strengthen gastrointestinal function and the ovarian function of laying hen, improves autoimmune
Power, promotes the growth of chickling, reduces sickness rate, improves the egg production of laying hen, and improves Eggshell weight and thickness, reduces soft shell
Egg, abnormal-shape egg and the generation of easy scrambled egg, improve color, smell and taste and the quality of egg；Disease can be prevented, exempting from of enhancing body simultaneously
Epidemic disease function, regulates internal beneficial microbe group, the flora environment of regulation the intestines and stomach, improves the digestion power of body.
It is a further object of the present invention to provide the preparation method of this laying hen Chinese medicine compound probiotic biological preparation.
To achieve these goals, the present invention adopts the following technical scheme that
A kind of laying hen Chinese medicine compound probiotic biological preparation, is prepared by composite probiotics ferment Chinese medicine mixed powder；
Described Chinese medicine mixed powder ratio by weight by Radix Astragali 10-20 part, Herb Gynostemmae Pentaphylli 10-20 part, Radix Isatidis 20-30 part,
Radix Angelicae Sinensis 5-8 part, Rhizoma Atractylodis Macrocephalae 5-8 part, Rhizoma Chuanxiong 5-8 part, Radix Dichroae 10-15 part, Herba Agrimoniae 10-15 part, Folium Ligustri Lucidi 20-30 part, Herba Pouzolziae Zeylanicae
5-8 part, Concretio Silicea Bambusae 5-8 part, Semen Vaccariae 7-9 part, Radix Pulsatillae 5-8 part, Folium Cinnamomi porrecti 10-20 part are prepared from；
Described probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed vaccine of cereuisiae fermentum composition
Group.
The preparation method of described Traditional Chinese drug mixture is as follows: each Chinese medicine powder is broken to 60-80 mesh, and mixing is put in extraction pot and added
Enter suitable quantity of water, soak 5-20 hour；It is then heated to 40-100 DEG C extract 10-15 hour, extracting solution is collected by filtration；Secondary is to medicine
Adding suitable quantity of water in slag, 40-100 DEG C is extracted 10-15 hour；It is then combined with twice extracting solution, in suction concentrating under reduced pressure tank, decompression
To 0.03-0.08MPa, temperature is maintained at 60-80 DEG C, and being concentrated into relative density is 1.20, the extractum of temperature to 60 DEG C-70 DEG C,
Cooling, drying and grinding becomes powder to prepare Chinese medicine mixed powder.
The described laying hen preparation method of Chinese medicine compound probiotic biological preparation, comprises the following steps:
(1) breeding:
1. prepared by culture medium: take glucose 5-10 part, peptone 6-8 part, agar 10-15 part, di(2-ethylhexyl)phosphate by weight ratio
Hydrogen potassium 1-2 part, sodium bicarbonate 2-3 part, distilled water 5-15 part, be configured to culture fluid；The culture fluid prepared is added material-compound tank
In, after being heated to boiling, regulation pH value proceeds in saccharifying tank to 4.5-5.0, adds saccharifying enzyme, protect at 60 DEG C in saccharifying tank
Temperature saccharifying 2-4h, obtains saccharified liquid, and wherein, the addition of saccharifying enzyme is 300-400U/g glucose；Finally saccharified liquid is proceeded to
In fluid heater, being warming up to 95-100 DEG C in 5-8s, be incubated 1-1.5h, high temperature sterilize, the saccharified liquid after sterilizing flows through fall
Temperature pipeline, is cooled to 30-40 DEG C, obtains breeding culture medium in 5-8s；
2. breeding culture medium is evenly distributed in four chemostats, by lactic acid bacteria good for laboratory cultures, hay spore
Bacillus, Rhodopseudomonas palustris, the strain of cereuisiae fermentum are respectively connected in four chemostats, constant temperature stirring training at 30-40 DEG C
Support 3 days, respectively obtain the ripe bacterium solution of lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, cereuisiae fermentum；Wherein, breast
Acid bacterium, bacillus subtilis, the inoculum concentration of Rhodopseudomonas palustris are 4-6%, and the inoculum concentration of cereuisiae fermentum is 5-7%；
(2) fermentation:
1. by lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the ripe bacterium solution of cereuisiae fermentum it is by volume
The ratio of 1: 0.5-0.8: 1-1.5: 1.2-1.8 is mixed and made into mixed bacteria liquid；Mixed bacteria liquid proceeds to fill the dilution of diluent
In tank, obtain mixed diluting bacterium solution；Described diluent is 1:1-2 with the weight ratio of mixed bacteria liquid；
2. the wheat bran of Chinese medicine mixed powder and its weight 20%, the bean cake powder of 5%, the sucrose of 2% are mixed homogeneously make solid
Body fermentation materials, loads solid fermentation material in steriliser, keeps 0.5-1h at 120 DEG C；
3. the solid fermentation material after sterilizing is joined in fermentation tank, in fermentation tank, then add mixed diluting bacterium
Liquid, 30-35 DEG C of bottom fermentation 25-30h, obtain semi-finished product；Described mixed diluting bacterium solution is 1 with the weight ratio of solid fermentation material:
1.5-2；
(3) it is dried, packs:
The most first semi-finished product are transported in the first drying machine, hot blast temperature 110-120 DEG C, are dried 3-5s；
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80-100 DEG C, is dried 3-4s；
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60-80 DEG C, is dried 2-4s；
4. the crushing material after being dried, to 60 mesh, through cooling conveying worm output, packaging, obtains finished product Chinese medicine
Compound probiotic biological preparation.
Step (2) 1. described in diluent component and content be: ferrous sulfate 0.02-0.03%, sulfadiazine
0.002-0.007%, manganese chloride 0.01-0.02%, citric acid 2%, sodium polyacrylate 1-5%, surplus is distilled water.
Each herbal pharmacology characteristic of Chinese medicine mixed powder of the present invention is as follows:
The Radix Astragali: sweet in the mouth is warm in nature, tonifying Qi and lifting yang, its active component astragalus polysaccharides can improve immunity of organisms；There is enhancing machine
Body immunity function, protects the liver, diuresis and wide antibacterial action.
Herb Gynostemmae Pentaphylli: toxin expelling, regulation immunologic function, its active component Total saponin, is three times of Radix Ginseng.
Radix Isatidis: there is heat-clearing and toxic substances removing, removing heat from blood antiinflammatory, its active component is indigo, indirubin etc. have resisting pathogenic microbes,
Strengthen immunologic function.
Radix Angelicae Sinensis: sweet in the mouth is warm in nature, can enrich blood, and can invigorate blood circulation again, its active component sodium ferulate energy activating macrophage, plays
Immunologic function.
The Rhizoma Atractylodis Macrocephalae: there is invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, the effect such as antiabortive.
Rhizoma Chuanxiong: there is blood-activating and qi-promoting, effect of wind-expelling pain-stopping；Radix Rehmanniae Preparata has the effect of YIN nourishing of enriching blood.
Radix Dichroae: can suppress or kill pathogen activity, excitating organism immunity containing the arteannuin in halofuginone hydrobromide, Herba Artemisiae Annuae
Power, strengthens leukocytic phagocytosis, promotes the effect that antibody is formed.
Herba Agrimoniae: herb is robustness astringent hemostatic, has heart tonifying, rises blood pressure, blood coagulation, hemostasis, removing heat from blood, antibacterial etc. makees
With.
The Folium Ligustri Lucidi: and Fructus Ligustri Lucidi contain equally triterpenes, secoiridiod, flavonoid, polysaccharide, phenylethanol,
The materials such as volatile oil, triterpenes components master contains ursolic acid and oleanolic acid, can remarkably promote the absorption of calcium, and preventing and treating growth promoter is not
Good and osteoporosis；Flavone content is equal with Fructus Ligustri Lucidi；Contained polysaccharide can improve the immunologic function of laying hen, and polysaccharide is also
Can reduce the harmful gass such as ammonia to produce, improve meat Egg Quality and fragrance, Folium Ligustri Lucidi price is less expensive.
Herba Pouzolziae Zeylanicae: nature and flavor: sweet is cool.Cure mainly: clearing away heat-damp and promoting diuresis, removing toxic substances and promoting pus discharge.For dysentery, enteritis, urinary tract infection；External
Control furuncle and phyma, mastitis.
Concretio Silicea Bambusae: pharmacological action: bacteriostatic experiment: all have stronger suppression to make common pyococcus and pathogenic entero becteria
With.Nature and flavor: sweet, cold.Curing mainly: clear heat and eliminate phlegm, mind calming is calmed the frightened.For calentura unconsciousness and delirium, middle wind-phlegm is stopped up, and infantile convulsion is twitched.
Semen Vaccariae: containing multiple Saponin, has promoting blood circulation to restore menstrual flow, reducing swelling and alleviating pain, expedites the emergence of the function of stimulating milk secretion, is clinical conventional
The Chinese medicine of stimulating milk secretion.
The Radix Pulsatillae: heat clearing and damp drying, removing pathogenic heat from blood and toxic substance from the body.
Compared with the prior art, beneficial effects of the present invention is as follows:
(1) Chinese medicinal components of the present invention is the growth characteristic according to laying hen, use monarch, minister, help, the drug matching that makes former
Then, choose respectively and can promote laying hen digestion and growth, laying hen immunity of organisms can be strengthened, laying hen resistance against diseases can be improved
A few herbs formulated, be possible not only to strengthen laying hen and protect the liver profit kidney and improve the function of ovary, cleaning vivotoxin and mistake
Quick material, reduces sickness rate, improves the egg production of egg, and improves Eggshell weight and thickness, reduces soft-shelled egg, abnormal-shape egg and easily
The generation of scrambled egg, improves color, smell and taste and the quality of egg；And the speed of growth of laying hen can be promoted, and laying hen can be strengthened
Disease resistance ability, even can also treat laying hen common disease.
(2) four kinds of probiotic bacterias of the present invention are according to optimum proportioning, form symbiosis, synergism, change the intestinal of body
Stomach microenvironment, is possible not only to promote intestinal mucosa reparative regeneration, reduces the probability of happening of various laying hen diarrhea disease；And enough promote
Many kinds of metal ions and the sorption enhanced of vitamin, to reach the purpose that nutrient substance makes full use of.Can be with structure between four kinds of bacterium
Become conspiracy relation, after certain proportion optimum organization, can breed rapidly in intestinal, improve the ratio of wherein probiotic bacteria,
And reduce the ratio of harmful bacteria.
(3) Chinese medicine is matched by the present invention with compound probiotic, can strengthen gastrointestinal function and the ovarian function of laying hen, carry
High autoimmunity, promotes the growth of chickling, reduces sickness rate, improves the egg production of laying hen, and improves Eggshell weight and thickness,
Reduce soft-shelled egg, abnormal-shape egg and the generation of easy scrambled egg, improve color, smell and taste and the quality of egg；Disease can be prevented simultaneously, strengthen
The immunologic function of body, regulates internal beneficial microbe group, the flora environment of regulation the intestines and stomach, improves the digestion power of body.
(4) preparation method of the present invention, by controlling the technological parameters such as each stage material input, temperature, time, it is possible to have
Effect ensures that temperature, time etc. are on product number of viable and the impact of biological effectiveness；The nutrition added in fermentation stage diluent
Material can promote the further increment of strain, and sodium polyacrylate plays good consolidation, to keep product forms；Later stage
Use staged cooling and drying, it is ensured that while effectively removing product moisture content, do not destroy the internal junction of compound probiotic
Structure, is particularly well-suited to large-scale industrial production.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but the present invention is not limited only to these embodiments, not
On the premise of departing from present inventive concept, within any improvement made all falls within protection scope of the present invention.
Embodiment 1:
A kind of laying hen Chinese medicine compound probiotic biological preparation, is prepared by composite probiotics ferment Chinese medicine mixed powder；
Described Chinese medicine mixed powder ratio by weight is by the Radix Astragali 10 parts, Herb Gynostemmae Pentaphylli 20 parts, Radix Isatidis 20 parts, Radix Angelicae Sinensis 5 parts, white
Art 8 parts, Rhizoma Chuanxiong 5 parts, Radix Dichroae 10 parts, Herba Agrimoniae 10 parts, the Folium Ligustri Lucidi 30 parts, Herba Pouzolziae Zeylanicae 5 parts, Concretio Silicea Bambusae 8 parts, Semen Vaccariae 7 parts,
The Radix Pulsatillae 5 parts, Folium Cinnamomi porrecti 10 parts are prepared from；
Described probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed vaccine of cereuisiae fermentum composition
Group.
The preparation method of described Traditional Chinese drug mixture is as follows: each Chinese medicine powder is broken to 60 mesh, and mixing is put in extraction pot and added
Suitable quantity of water, soaks 5 hours；It is then heated to 40 DEG C extract 15 hours, extracting solution is collected by filtration；Secondary adds in medicinal residues in right amount
Water, 40 DEG C are extracted 15 hours；Being then combined with twice extracting solution, in suction concentrating under reduced pressure tank, be decompressed to 0.03MPa, temperature keeps
At 80 DEG C, being concentrated into relative density is 1.20, the extractum of temperature to 60 DEG C, cooling, and drying and grinding becomes powder to prepare Chinese medicine mixing
Powder.
The described laying hen preparation method of Chinese medicine compound probiotic biological preparation, comprises the following steps:
(1) breeding:
1. prepared by culture medium: take by weight ratio glucose 5 parts, peptone 6 parts, 15 parts of agar, potassium dihydrogen phosphate 1 part,
Sodium bicarbonate 3 parts, distilled water 5 parts, be configured to culture fluid；The culture fluid prepared is added in material-compound tank, after being heated to boiling,
Regulation pH value proceeds in saccharifying tank after 4.5, adds saccharifying enzyme in saccharifying tank, is incubated saccharifying 2h, obtains saccharified liquid at 60 DEG C,
Wherein, the addition of saccharifying enzyme is 300U/g glucose；Finally saccharified liquid is proceeded in fluid heater, in 5s, be warming up to 95
DEG C, it being incubated 1.5h, high temperature sterilize, the saccharified liquid after sterilizing flows through cooling pipeline, is cooled to 30 DEG C in 5s, obtains breeding and cultivates
Base；
2. breeding culture medium is evenly distributed in four chemostats, by lactic acid bacteria good for laboratory cultures, hay spore
Bacillus, Rhodopseudomonas palustris, the strain of cereuisiae fermentum are respectively connected in four chemostats, constant temperature stir culture 3 at 30 DEG C
My god, respectively obtain the ripe bacterium solution of lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, cereuisiae fermentum；Wherein, lactic acid
Bacterium, bacillus subtilis, the inoculum concentration of Rhodopseudomonas palustris are 4%, and the inoculum concentration of cereuisiae fermentum is 5%；
(2) fermentation:
1. by lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the ripe bacterium solution of cereuisiae fermentum it is by volume
The ratio of 1: 0.5: 1: 1.2 is mixed and made into mixed bacteria liquid；Proceed to mixed bacteria liquid to fill in the thinning tank of diluent, mixed
Dilution bacterium solution；Described diluent is 1:1 with the weight ratio of mixed bacteria liquid；Described diluent component and content is: ferrous sulfate
0.02%, sulfadiazine 0.002%, manganese chloride 0.02%, citric acid 2%, sodium polyacrylate 1%, surplus is distilled water；
2. the wheat bran of Chinese medicine mixed powder and its weight 20%, the bean cake powder of 5%, the sucrose of 2% are mixed homogeneously make solid
Body fermentation materials, loads solid fermentation material in steriliser, keeps 0.5h at 120 DEG C；
3. the solid fermentation material after sterilizing is joined in fermentation tank, in fermentation tank, then add mixed diluting bacterium
Liquid, 30 DEG C of bottom fermentation 30h, obtain semi-finished product；Described mixed diluting bacterium solution is 1:1.5 with the weight ratio of solid fermentation material；
(3) it is dried, packs:
The most first semi-finished product are transported in the first drying machine, hot blast temperature 110 DEG C, are dried 5s；
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80 DEG C, is dried 4s；
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60 DEG C, is dried 4s；
4. the crushing material after being dried, to 60 mesh, through cooling conveying worm output, packaging, obtains finished product Chinese medicine
Compound probiotic biological preparation.
Embodiment 2:
A kind of laying hen Chinese medicine compound probiotic biological preparation, is prepared by composite probiotics ferment Chinese medicine mixed powder；
Described Chinese medicine mixed powder ratio by weight is by the Radix Astragali 15 parts, Herb Gynostemmae Pentaphylli 15 parts, Radix Isatidis 25 parts, Radix Angelicae Sinensis 6 parts, white
Art 6 parts, Rhizoma Chuanxiong 6 parts, Radix Dichroae 12 parts, Herba Agrimoniae 12 parts, the Folium Ligustri Lucidi 25 parts, Herba Pouzolziae Zeylanicae 6 parts, Concretio Silicea Bambusae 6 parts, Semen Vaccariae 8 parts,
The Radix Pulsatillae 7 parts, Folium Cinnamomi porrecti 15 parts are prepared from；
Described probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed vaccine of cereuisiae fermentum composition
Group.
The preparation method of described Traditional Chinese drug mixture is as follows: each Chinese medicine powder is broken to 70 mesh, and mixing is put in extraction pot and added
Suitable quantity of water, soaks 10 hours；It is then heated to 80 DEG C extract 12 hours, extracting solution is collected by filtration；Secondary adds suitable in medicinal residues
Amount water, 80 DEG C are extracted 12 hours；Being then combined with twice extracting solution, in suction concentrating under reduced pressure tank, be decompressed to 0.05MPa, temperature is protected
Holding at 70 DEG C, being concentrated into relative density is 1.20, the extractum of temperature to 65 DEG C, cooling, and it is mixed that drying and grinding becomes powder to prepare Chinese medicine
Close powder.
The described laying hen preparation method of Chinese medicine compound probiotic biological preparation, comprises the following steps:
(1) breeding:
1. prepared by culture medium: take by weight ratio glucose 8 parts, peptone 7 parts, 12 parts of agar, potassium dihydrogen phosphate 2 parts,
Sodium bicarbonate 3 parts, distilled water 10 parts, be configured to culture fluid；The culture fluid prepared is added in material-compound tank, is heated to boiling
After, regulation pH value proceeds in saccharifying tank after 5.0, adds saccharifying enzyme in saccharifying tank, is incubated saccharifying 3h, obtains saccharifying at 60 DEG C
Liquid, wherein, the addition of saccharifying enzyme is 400U/g glucose；Finally saccharified liquid is proceeded in fluid heater, heat up in 6s
To 100 DEG C, being incubated 1.5h, high temperature sterilize, the saccharified liquid after sterilizing flows through cooling pipeline, is cooled to 35 DEG C, obtains breeding in 6s
Culture medium；
2. breeding culture medium is evenly distributed in four chemostats, by lactic acid bacteria good for laboratory cultures, hay spore
Bacillus, Rhodopseudomonas palustris, the strain of cereuisiae fermentum are respectively connected in four chemostats, constant temperature stir culture 3 at 35 DEG C
My god, respectively obtain the ripe bacterium solution of lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, cereuisiae fermentum；Wherein, lactic acid
Bacterium, bacillus subtilis, the inoculum concentration of Rhodopseudomonas palustris are 5%, and the inoculum concentration of cereuisiae fermentum is 6%；
(2) fermentation:
1. by lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the ripe bacterium solution of cereuisiae fermentum it is by volume
The ratio of 1: 0.6: 1.2: 1.5 is mixed and made into mixed bacteria liquid；Proceed to mixed bacteria liquid to fill in the thinning tank of diluent, mixed
Close dilution bacterium solution；Described diluent is 1:2 with the weight ratio of mixed bacteria liquid；Described diluent component and content is: ferrous sulfate
0.03%, sulfadiazine 0.005%, manganese chloride 0.02%, citric acid 2%, sodium polyacrylate 3%, surplus is distilled water.
2. the wheat bran of Chinese medicine mixed powder and its weight 20%, the bean cake powder of 5%, the sucrose of 2% are mixed homogeneously make solid
Body fermentation materials, loads solid fermentation material in steriliser, keeps 1h at 120 DEG C；
3. the solid fermentation material after sterilizing is joined in fermentation tank, in fermentation tank, then add mixed diluting bacterium
Liquid, 35 DEG C of bottom fermentation 30h, obtain semi-finished product；Described mixed diluting bacterium solution is 1:2 with the weight ratio of solid fermentation material；
(3) it is dried, packs:
The most first semi-finished product are transported in the first drying machine, hot blast temperature 120 DEG C, are dried 5s；
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 100 DEG C, is dried 4s；
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 70 DEG C, is dried 3s；
4. the crushing material after being dried, to 60 mesh, through cooling conveying worm output, packaging, obtains finished product Chinese medicine
Compound probiotic biological preparation.
Embodiment 3:
A kind of laying hen Chinese medicine compound probiotic biological preparation, is prepared by composite probiotics ferment Chinese medicine mixed powder；
Described Chinese medicine mixed powder ratio by weight is by the Radix Astragali 20 parts, Herb Gynostemmae Pentaphylli 20 parts, Radix Isatidis 30 parts, Radix Angelicae Sinensis 5 parts, white
Art 8 parts, Rhizoma Chuanxiong 8 parts, Radix Dichroae 15 parts, Herba Agrimoniae 15 parts, the Folium Ligustri Lucidi 20 parts, Herba Pouzolziae Zeylanicae 8 parts, Concretio Silicea Bambusae 8 parts, Semen Vaccariae 9 parts,
The Radix Pulsatillae 8 parts, Folium Cinnamomi porrecti 20 parts are prepared from；
Described probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed vaccine of cereuisiae fermentum composition
Group.
The preparation method of described Traditional Chinese drug mixture is as follows: each Chinese medicine powder is broken to 80 mesh, and mixing is put in extraction pot and added
Suitable quantity of water, soaks 20 hours；It is then heated to 100 DEG C extract 10 hours, extracting solution is collected by filtration；Secondary adds suitable in medicinal residues
Amount water, 100 DEG C are extracted 10 hours；Being then combined with twice extracting solution, in suction concentrating under reduced pressure tank, be decompressed to 0.08MPa, temperature is protected
Holding at 80 DEG C, being concentrated into relative density is 1.20, the extractum of temperature to 70 DEG C, cooling, and it is mixed that drying and grinding becomes powder to prepare Chinese medicine
Close powder.
The described laying hen preparation method of Chinese medicine compound probiotic biological preparation, comprises the following steps:
(1) breeding:
1. prepared by culture medium: take by weight ratio glucose 10 parts, peptone 8 parts, 15 parts of agar, potassium dihydrogen phosphate 2 parts,
Sodium bicarbonate 2 parts, distilled water 15 parts, be configured to culture fluid；The culture fluid prepared is added in material-compound tank, is heated to boiling
After, regulation pH value proceeds in saccharifying tank after 5.0, adds saccharifying enzyme in saccharifying tank, is incubated saccharifying 4h, obtains saccharifying at 60 DEG C
Liquid, wherein, the addition of saccharifying enzyme is 400U/g glucose；Finally saccharified liquid is proceeded in fluid heater, heat up in 8s
To 100 DEG C, being incubated 1h, high temperature sterilize, the saccharified liquid after sterilizing flows through cooling pipeline, is cooled to 40 DEG C in 8s, obtains breeding training
Support base；
2. breeding culture medium is evenly distributed in four chemostats, by lactic acid bacteria good for laboratory cultures, hay spore
Bacillus, Rhodopseudomonas palustris, the strain of cereuisiae fermentum are respectively connected in four chemostats, constant temperature stir culture 3 at 40 DEG C
My god, respectively obtain the ripe bacterium solution of lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, cereuisiae fermentum；Wherein, lactic acid
Bacterium, bacillus subtilis, the inoculum concentration of Rhodopseudomonas palustris are 6%, and the inoculum concentration of cereuisiae fermentum is 7%；
(2) fermentation:
1. by lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the ripe bacterium solution of cereuisiae fermentum it is by volume
The ratio of 1: 0.8: 1.5: 1.8 is mixed and made into mixed bacteria liquid；Proceed to mixed bacteria liquid to fill in the thinning tank of diluent, mixed
Close dilution bacterium solution；Described diluent is 1:2 with the weight ratio of mixed bacteria liquid；Described diluent component and content is: ferrous sulfate
0.03%, sulfadiazine 0.007%, manganese chloride 00.02%, citric acid 2%, sodium polyacrylate 5%, surplus is distilled water.
2. the wheat bran of Chinese medicine mixed powder and its weight 20%, the bean cake powder of 5%, the sucrose of 2% are mixed homogeneously make solid
Body fermentation materials, loads solid fermentation material in steriliser, keeps 1h at 120 DEG C；
3. the solid fermentation material after sterilizing is joined in fermentation tank, in fermentation tank, then add mixed diluting bacterium
Liquid, 35 DEG C of bottom fermentation 30h, obtain semi-finished product；Described mixed diluting bacterium solution is 1:2 with the weight ratio of solid fermentation material；
(3) it is dried, packs:
The most first semi-finished product are transported in the first drying machine, hot blast temperature 120 DEG C, are dried 5s；
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 100 DEG C, is dried 3s；
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 80 DEG C, is dried 4s；
4. the crushing material after being dried, to 60 mesh, through cooling conveying worm output, packaging, obtains finished product Chinese medicine
Compound probiotic biological preparation.
Test example:
(1) toxicity test
Select SD rat, give the Chinese medicine compound probiotic biological preparation in the embodiment of the present invention 2, gavage every day after molten water
Once, continuous 90 days, last was administered latter 24 hours respectively to organize to live and kills 1/2 animal (male and female half and half), and remaining 1/2 animal continues observation 2
Live after week and kill.Observe the outward appearance of animal, general behavior, food ration, body weight change during test, be administered latter 90 days and drug withdrawal 2 weeks
Carry out hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN,
Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, the index checking such as histopathology.Result of the test shows:
The Chinese medicine compound probiotic biological preparation of the present invention is feeding period, and rat general state is good, outward appearance sign, behavioral activity,
Food-intake and body weight increase change the most without exception；Hematological examination, blood biochemical analysis, urine biochemical analysis are all in normal range；
Main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.According to result of the test originally
Continuous 90 days of the Chinese medicine compound probiotic biological preparation of invention is administered and has no significant effect rat, without clear and definite toxicity target organ and
Sensitive indicator, convalescent period observation also has no retardance toxic reaction, the dosage peace of the pharmaceutical preparation clinical practice of the prompting present invention
Full property is higher.
(2) contrast test
1, test and Selection 150 age in days laying hen 200, is randomly divided into 4 test group, often group 50, respectively A, B, C, D
Group, the cultivation phase is 40 days.
A group: laying hen conventional feed.
B group: the Chinese medicine compound probiotic biological preparation described in embodiment 1 of laying hen conventional feed and feed relative 2%.
C group: the Chinese medicine compound probiotic biological preparation described in embodiment 2 of laying hen conventional feed and feed relative 2%.
D group: the Chinese medicine compound probiotic biological preparation described in embodiment 3 of laying hen conventional feed and feed relative 2%.
2, feeding and management and index determining
All test chickens are all raised in same hen house, and immune programme for children and feeding and management method are carried out in the usual way.
Day feeding 2 times, doses is sufficient, freely drinks water, longitudinal direction ventilation fan, illumination every day 16 hours, illumination every cubic metre 3～
4 watts.Scrape excrement every day 1 time.Record laying rate, average egg weight, broken soft egg rate and laying hen death rate.
3, result of the test see table 1:
The impact on Layer Production Performance of the Chinese medicine compound probiotic biological preparation of table 1 embodiment 1,2,3
As seen from the above table, in laying hen conventional feed, add the Chinese medicine compound probiotic of the present invention biology of 0.2%
Preparation, can obviously reduce laying hen death rate, improves laying rate, increases egg size, improves the quality of egg.
Claims (4)
1. a laying hen Chinese medicine compound probiotic biological preparation, it is characterised in that: by composite probiotics ferment Chinese medicine mixed powder
Prepare；
Described Chinese medicine mixed powder ratio by weight is by Radix Astragali 10-20 part, Herb Gynostemmae Pentaphylli 10-20 part, Radix Isatidis 20-30 part, Radix Angelicae Sinensis
5-8 part, Rhizoma Atractylodis Macrocephalae 5-8 part, Rhizoma Chuanxiong 5-8 part, Radix Dichroae 10-15 part, Herba Agrimoniae 10-15 part, Folium Ligustri Lucidi 20-30 part, Herba Pouzolziae Zeylanicae 5-8
Part, Concretio Silicea Bambusae 5-8 part, Semen Vaccariae 7-9 part, Radix Pulsatillae 5-8 part, Folium Cinnamomi porrecti 10-20 part are prepared from；
Described probiotic bacteria is lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the mixed bacterial of cereuisiae fermentum composition.
Laying hen the most according to claim 1 Chinese medicine compound probiotic biological preparation, it is characterised in that: described Chinese medicine mixes
The preparation method of thing is as follows: each Chinese medicine powder is broken to 60-80 mesh, and addition suitable quantity of water in extraction pot is put in mixing, soaks 5-20 little
Time；It is then heated to 40-100 DEG C extract 10-15 hour, extracting solution is collected by filtration；Secondary adds suitable quantity of water, 40-in medicinal residues
100 DEG C are extracted 10-15 hour；It is then combined with twice extracting solution, in suction concentrating under reduced pressure tank, is decompressed to 0.03-0.08MPa, temperature
Degree is maintained at 60-80 DEG C, and being concentrated into relative density is 1.20, the extractum of temperature to 60 DEG C-70 DEG C, and cooling, drying and grinding becomes powder
End prepares Chinese medicine mixed powder.
3. the laying hen as claimed in claim 1 preparation method of Chinese medicine compound probiotic biological preparation, it is characterised in that:
Comprise the following steps:
(1) breeding:
1. prepared by culture medium: take glucose 5-10 part, peptone 6-8 part, agar 10-15 part, potassium dihydrogen phosphate by weight ratio
1-2 part, sodium bicarbonate 2-3 part, distilled water 5-15 part, be configured to culture fluid；The culture fluid prepared is added in material-compound tank, adds
After heat extremely boiling, regulation pH value proceeds in saccharifying tank to 4.5-5.0, adds saccharifying enzyme in saccharifying tank, and at 60 DEG C, insulation is sugared
Changing 2-4h, obtain saccharified liquid, wherein, the addition of saccharifying enzyme is 300-400U/g glucose；Finally saccharified liquid is proceeded to fluid
In heater, being warming up to 95-100 DEG C in 5-8s, be incubated 1-1.5h, high temperature sterilize, the saccharified liquid after sterilizing flows through cooling pipe
Road, is cooled to 30-40 DEG C in 5-8s, obtain breeding culture medium；
2. breeding culture medium is evenly distributed in four chemostats, by lactic acid bacteria good for laboratory cultures, bacillus subtilis,
Rhodopseudomonas palustris, the strain of cereuisiae fermentum are respectively connected in four chemostats, constant temperature stir culture 3 days at 30-40 DEG C,
Respectively obtain the ripe bacterium solution of lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, cereuisiae fermentum；Wherein, lactic acid bacteria,
Bacillus subtilis, the inoculum concentration of Rhodopseudomonas palustris are 4-6%, and the inoculum concentration of cereuisiae fermentum is 5-7%；
(2) fermentation:
1. it is 1 by volume by lactic acid bacteria, bacillus subtilis, Rhodopseudomonas palustris, the ripe bacterium solution of cereuisiae fermentum:
The ratio of 0.5-0.8: 1-1.5: 1.2-1.8 is mixed and made into mixed bacteria liquid；Mixed bacteria liquid proceeds to fill the thinning tank of diluent
In, obtain mixed diluting bacterium solution；Described diluent is 1:1-2 with the weight ratio of mixed bacteria liquid；
2. the wheat bran of Chinese medicine mixed powder and its weight 20%, the bean cake powder of 5%, the sucrose of 2% are mixed homogeneously and make solid and send out
Ferment material, loads solid fermentation material in steriliser, keeps 0.5-1h at 120 DEG C；
3. the solid fermentation material after sterilizing is joined in fermentation tank, in fermentation tank, then add mixed diluting bacterium solution, 30-
35 DEG C of bottom fermentation 25-30h, obtain semi-finished product；Described mixed diluting bacterium solution is 1:1.5-2 with the weight ratio of solid fermentation material；
(3) it is dried, packs:
The most first semi-finished product are transported in the first drying machine, hot blast temperature 110-120 DEG C, are dried 3-5s；
2. the material of the first drying machine output enters the second drying machine, and hot blast temperature is 80-100 DEG C, is dried 3-4s；
3. the material of the second drying machine output enters the 3rd drying machine, and hot blast temperature is 60-80 DEG C, is dried 2-4s；
4. the crushing material after being dried, to 60 mesh, through cooling conveying worm output, packaging, obtains finished product Chinese medicine and is combined
Probiotic bacteria biological preparation.
The described laying hen preparation method of Chinese medicine compound probiotic biological preparation the most according to claim 3, its feature
Be: step (2) 1. described in diluent component and content be: ferrous sulfate 0.02-0.03%, sulfadiazine 0.002-
0.007%, manganese chloride 0.01-0.02%, citric acid 2%, sodium polyacrylate 1-5%, surplus is distilled water.
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| CN109198233A (en) * | 2018-08-02 | 2019-01-15 | 河南牧翔动物药业有限公司 | A kind of raising performance in layers and the feed addictive of immune function and preparation method thereof |
| CN111557393A (en) * | 2020-06-15 | 2020-08-21 | 安徽农业大学 | Composite probiotic for improving laying performance of laying hens and preparation method thereof |
| CN113974012A (en) * | 2021-09-30 | 2022-01-28 | 江门市旺海饲料实业有限公司 | Anti-coccidiosis feed additive and preparation method and application thereof |
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