CN106172216A - 一种防治鸭病毒性肝炎的养鸭方法 - Google Patents
一种防治鸭病毒性肝炎的养鸭方法 Download PDFInfo
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- CN106172216A CN106172216A CN201610594712.XA CN201610594712A CN106172216A CN 106172216 A CN106172216 A CN 106172216A CN 201610594712 A CN201610594712 A CN 201610594712A CN 106172216 A CN106172216 A CN 106172216A
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- Medicines Containing Plant Substances (AREA)
Abstract
本发明属于家禽养殖领域,具体公开了一种防治鸭病毒性肝炎的养鸭方法,对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于小鸭,根据日龄,饲喂一定量的防病鸭饲料,且3次/天,同时按一定规律对鸭皮下注射一定量的防病注射液;且每次饲喂后都将鸭子放养到无公害稻田中。其中,防病鸭饲料由能量饲料、微量元素添加剂和中草药添加剂按照一定重量份比制成;中草药添加剂和防病注射液由虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗按照一定重量份比制成。本发明的养鸭方法,不仅成本低廉、易于实施,而且安全无副作用、无药残,可以保证出栏鸭肉的质量。
Description
【技术领域】
本发明涉及家禽养殖技术领域,具体涉及一种防治鸭病毒性肝炎的养鸭方法。
【背景技术】
鸭病毒性肝炎是由鸭肝炎病毒引起雏鸭的一种急性接触性传染病临床上以发病急、传播快、死亡率高,剖检肝脏有明显出血点和出血斑为特征。鸭病毒性肝炎是雏鸭的种传播迅速和高度致死的传染病。肝脏的病变特征是体积增大和有出血斑点,开始发病时的死亡率高达90%以上,造成很大损失。本病主要发生于3周龄以下的雏鸭,4周龄以上尚未见到发病报道,对成年鸭没有影响,鸡和鹅也都有抵抗力。
目前,对鸭病毒性肝炎主要是用疫苗预防和应用鸭病毒性肝炎卵黄抗体。而免疫用疫苗常因保存和使用不当而导致免疫失败,有些地区因免疫密度不高而引起流行,给养鸭业造成严重的经济损失;应用鸭病毒性肝炎卵黄抗体,又因价格昂贵应用较少;还有一种是使用高免血清和单克隆抗体来预防鸭病毒性肝炎,但又因资源紧缺、价格昂贵,致使实际应用受到限制。
【发明内容】
本发明的发明目的在于:针对上述存在的问题,提供一种防治鸭病毒性肝炎的养鸭方法。该方法采用绿色安全的中草药为添加剂原料,不仅成本低廉,能够协同起到清热解毒、补脾益气、抑制病毒、保肝利胆的作用,从而达到有效防治鸭病毒性肝炎的目的,而且安全无副作用、无药残,可以保证出栏鸭肉的质量。
为了实现上述目的,本发明采用的技术方案如下:
一种防治鸭病毒性肝炎的养鸭方法,对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于日龄在1-8天以内的小鸭,对鸭皮下注射防病注射液,2天/次,且每次0.4mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.2-0.4g;对于日龄在9-20天以内的小鸭,对鸭皮下注射防病注射液,5天/次,且每次0.3mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.4-0.5g;对于日龄在20天以上的小鸭,用防病鸭饲料进行饲喂直至出栏,且3次/天,每次让其自由采食至吃饱为止;且每次饲喂后都将鸭子放养到无公害稻田中;
所述防病鸭饲料由能量饲料250-350份、微量元素添加剂1-3份和中草药添加剂8-20份组成;
所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖12-26份、赤芍12-26份、白花蛇舌草12-24份、草决明12-24份、柴胡12-22份、大青叶8-19份、丁葵草8-19份、柏子仁8-17份、茯苓5-16份、甘草5-13份和橘梗3-11份。
在本发明中,进一步的,所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖17-22份、赤芍17-21份、白花蛇舌草16-19份、草决明15-19份、柴胡14-17份、大青叶11-14份、丁葵草11-13份、柏子仁11-13份、茯苓7-10份、甘草7-9份和橘梗6-9份。
在本发明中,进一步的,所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖18份、赤芍18份、白花蛇舌草17份、草决明17份、柴胡15份、大青叶12份、丁葵草12份、柏子仁12份、茯苓9份、甘草8份和橘梗7份。
在本发明中,进一步的,所述能量饲料由玉米粉30-55份、绿豆粉24-43份和鱼粉18-27份混合至均匀后,经过粗粉碎过筛80目制成;所述的微量元素添加剂由贝壳粉2-4份和松花粉1-3份混合至均匀制成。
在本发明中,进一步的,所述防病鸭饲料由以下具体步骤制成:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85-90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液置于真空条件下,经过浓缩并雾化干燥后,粗粉碎并过100目筛,即得到中药粉,备用;
(3)按重量份计,将(2)所得中药粉与能量饲料、微量元素添加剂搅拌至混合均匀,经过低温超微粉碎并过200目筛后,投入制粒机制成颗粒,包装后即可得到成品。
进一步的,步骤(3)中,所述颗粒的直径为2.8-4.0mm。
在本发明中,进一步的,所述防病注射液由以下具体步骤制成:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85-90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液放入离心机中以2000r/min的速度离心30min,即得中药液,备用;
(3)将(2)中的中药液置于真空条件下,浓缩至含水量为12-16%的稠膏,将所得的稠膏放至室温后,加入体积为6-11%的丙三醇,并加与稠膏和丙三醇相同体积的生理盐水,搅拌至混合均匀,于2℃冷藏静置40小时后过滤,得滤液;所得滤液再依次经0.45μm、0.22μm的微孔滤膜循环过滤2次,经灌封、灭菌后即得本发明1g生药/mL的防病注射液。
综上所述,由于采用了上述技术方案,本发明的有益效果是:
(1)本发明的养鸭方法,通过按一定的规律向种鸭和小鸭饲喂一定量防病鸭饲料来防治鸭病毒性肝炎,而防病鸭饲料中添加了中草药,不仅具有绿色安全、实施方便且成本低廉的优点,而且可以真正从鸭体内内调养,增强鸭自身的免疫力,提高抗病能力,且添加的各个中草药协同达到清热解毒、补脾益气、抑制病毒、保肝利胆的作用,让小鸭从母体开始就得到保护,从而可以从日常饲喂中达到有效防治鸭病毒性肝炎的目的;且添加了以贝壳粉和松花粉组成的微量元素添加剂,不仅可以有效的补充鸭所必需的微量元素和氨基酸,还可以增加饲料的香味、增强消化能力,提高鸭的采食率,从而提高日增重率。
(2)本发明采用绿色安全的中草药作为原料,绿色安全,不仅解决了使用疫苗产生的高费用和药残问题,而且可以有效防治鸭病毒性肝炎,保证鸭肉的质量。本配方的各个中草药配方合理,以虎杖、赤芍、白花蛇舌草、草决明和柴胡为主,大青叶、丁葵草和柏子仁为辅,茯苓和甘草为佐,橘梗为使,苦寒和甘温的性味相协调,达到性味平和,且相互作用,协同达到清热解毒、补脾益气、抑制病毒、保肝利胆的作用,从而可以有效防治鸭病毒性肝炎。各个中草药的药理和药性如下:
虎杖:微苦微寒,具有清热解毒、利胆退黄、祛风利湿、散瘀定痛和止咳化痰的作用。用于湿热黄疸、产后瘀血不下、咳嗽痰多、水火烫伤、跌打损伤和痈肿疮毒等病症。
赤芍:味苦性微寒,有行瘀、止痛、凉血、消肿、抑制病毒、保肝利胆的功效,临床常用治疗瘀滞经闭、积聚、腹痛胁痛等病症。
白花蛇舌草:甘寒无毒,具有清热解毒、消痈利湿的功效,适用于痈肿疮毒、咽喉肿痛、淋证、黄疸及蛇虫咬伤等病症
草决明:味苦、甘而性凉,具有清肝火、祛风湿、益肾明目、泄泻和降血压等功能,可治风热赤眼、青盲、雀目、高血压、肝炎、肝硬化腹水和习惯性便秘等病症。
柴胡:气微香、味微苦,具有清虚热的作用,用于感冒发热、寒热往来、疟疾、肝郁气滞、胸肋胀痛、脱肛、子宫脱落、***等病症。
大青叶:味苦性寒,具有清热解毒、凉血止血的作用,可用于治温病热盛烦渴、流行性感冒和急性传染性肝炎等病症。
丁葵草:味甘、淡,性凉,具有祛瘀消肿和调起利湿的作用,可治感冒、胃肠炎、肝炎、痢疾、小儿惊风和颈***等病症。
柏子仁:性平味甘,具有养心安神、润肠通便的功效,可治惊悸、失眠、遗精、盗汗、便秘等症。
茯苓:味甘淡、性平,具有淡渗利湿、解毒散结、祛风通络和利湿泄浊的功效,主治梅毒、、癌瘤、筋骨挛痛、水肿、泄泻脚气和湿疹疥癣等病症。
甘草:味甘性平,具有清火、清热解毒、润肺止咳和补脾益气的功效,主治咽喉肿痛、痛疽疮疡、胃肠道溃疡以及解药毒、食物中毒等。
橘梗:性平、味苦、辛,具有开宣肺气、祛痰、排脓的功效,适用于外感风寒、咽喉肿痛、肺脓疡、咳吐脓血和痢痰腹痛等症。
【具体实施方式】
以下结合实施例对本发明作进一步说明。
实施例1
1.按重量份计,称取下列原料:
能量饲料:250份;微量元素添加剂:1份;
中草药添加剂:8份。
其中,能量饲料由以下重量份的原料组成:
玉米粉:30份; 绿豆粉:24份;
鱼粉:18份。
微量元素添加剂由以下重量份的原料组成:
贝壳粉:2份;松花粉:1份。
中草药添加剂由以下重量份的原料组成:
2.按以下具体步骤制取防病鸭饲料:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液置于真空条件下,经过浓缩并雾化干燥后,粗粉碎并过100目筛,即得到中药粉,备用;
(3)按重量份计,将(2)所得中药粉与能量饲料、微量元素添加剂搅拌至混合均匀,经过低温超微粉碎并过200目筛后,投入制粒机制成直径为2.8mm的颗粒,包装后即可得到成品。
3.按以下具体步骤制取防病注射液:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液放入离心机中以2000r/min的速度离心30min,即得中药液,备用;
(3)将(2)中的中药液置于真空条件下,浓缩至含水量为12%的稠膏,将所得的稠膏放至室温后,加入体积为6%的丙三醇,并加与稠膏和丙三醇相同体积的生理盐水,搅拌至混合均匀,于2℃冷藏静置40小时后过滤,得滤液;所得滤液再依次经0.45μm、0.22μm的微孔滤膜循环过滤2次,经灌封、灭菌后即得本发明1g生药/mL的防病注射液。
4.按以下具体方法对鸭进行饲喂;
对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于日龄在1-8天以内的小鸭,对鸭皮下注射防病注射液,2天/次,且每次0.4mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.2g;对于日龄在9-20天以内的小鸭,对鸭皮下注射防病注射液,5天/次,且每次0.3mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.4g;对于日龄在20天以上的小鸭,用防病鸭饲料进行饲喂直至出栏,且3次/天,每次让其自由采食至吃饱为止;且每次饲喂后都将鸭子放养到无公害稻田中。
实施例2
1.按重量份计,称取下列原料:
能量饲料:300份;微量元素添加剂:2份;
中草药添加剂:14份。
其中,能量饲料由以下重量份的原料组成:
玉米粉:45份;绿豆粉:38份;
鱼粉:20份。
微量元素添加剂由以下重量份的原料组成:
贝壳粉:3份;松花粉:2份。
中草药添加剂由以下重量份的原料组成:
2.按以下具体步骤制取防病鸭饲料:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为87%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液置于真空条件下,经过浓缩并雾化干燥后,粗粉碎并过100目筛,即得到中药粉,备用;
(3)按重量份计,将(2)所得中药粉与能量饲料、微量元素添加剂搅拌至混合均匀,经过低温超微粉碎并过200目筛后,投入制粒机制成直径为3.3mm的颗粒,包装后即可得到成品。
3.按以下具体步骤制取防病注射液:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为87%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液放入离心机中以2000r/min的速度离心30min,即得中药液,备用;
(3)将(2)中的中药液置于真空条件下,浓缩至含水量为14%的稠膏,将所得的稠膏放至室温后,加入体积为10%的丙三醇,并加与稠膏和丙三醇相同体积的生理盐水,搅拌至混合均匀,于2℃冷藏静置40小时后过滤,得滤液;所得滤液再依次经0.45μm、0.22μm的微孔滤膜循环过滤2次,经灌封、灭菌后即得本发明1g生药/mL的防病注射液。
4.按以下具体方法对鸭进行饲喂;
对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于日龄在1-8天以内的小鸭,对鸭皮下注射防病注射液,2天/次,且每次0.4mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.3g;对于日龄在9-20天以内的小鸭,对鸭皮下注射防病注射液,5天/次,且每次0.3mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.45g;对于日龄在20天以上的小鸭,用防病鸭饲料进行饲喂直至出栏,且3次/天,每次让其自由采食至吃饱为止;且每次饲喂后都将鸭子放养到无公害稻田中。
实施例3
1.按重量份计,称取下列原料:
能量饲料:350份;微量元素添加剂:3份;
中草药添加剂:20份。
微量元素添加剂由以下重量份的原料组成:
贝壳粉:4份;松花粉:3份。
其中,能量饲料由以下重量份的原料组成:
玉米粉:55份;绿豆粉:43份;
鱼粉:27份。
中草药添加剂由以下重量份的原料组成:
2.按以下具体步骤制取防病鸭饲料:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液置于真空条件下,经过浓缩并雾化干燥后,粗粉碎并过100目筛,即得到中药粉,备用;
(3)按重量份计,将(2)所得中药粉与能量饲料、微量元素添加剂搅拌至混合均匀,经过低温超微粉碎并过200目筛后,投入制粒机制成直径为4.0mm的颗粒,包装后即可得到成品。
3.按以下具体步骤制取防病注射液:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液放入离心机中以2000r/min的速度离心30min,即得中药液,备用;
(3)将(2)中的中药液置于真空条件下,浓缩至含水量为16%的稠膏,将所得的稠膏放至室温后,加入体积为11%的丙三醇,并加与稠膏和丙三醇相同体积的生理盐水,搅拌至混合均匀,于2℃冷藏静置40小时后过滤,得滤液;所得滤液再依次经0.45μm、0.22μm的微孔滤膜循环过滤2次,经灌封、灭菌后即得本发明1g生药/mL的防病注射液。
4.按以下具体方法对鸭进行饲喂;
对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于日龄在1-8天以内的小鸭,对鸭皮下注射防病注射液,2天/次,且每次0.4mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.4g;对于日龄在9-20天以内的小鸭,对鸭皮下注射防病注射液,5天/次,且每次0.3mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.5g;对于日龄在20天以上的小鸭,用防病鸭饲料进行饲喂直至出栏,且3次/天,每次让其自由采食至吃饱为止;且每次饲喂后都将鸭子放养到无公害稻田中。
效果验证:从某养鸭场选出80只种鸭,这80只种鸭无明显的生态学差异,经其平均分为2组。一组使用常规饲料进行饲喂,作为对照组;一组使用本发明的防病鸭饲料进行饲喂,作为实验组。两组鸭所在的外部环境一致,经过饲喂直至孵化出小鸭。统计种鸭和孵化出小鸭的鸭病毒性肝炎的发病率和死亡率,结果见表1:
表1
由上表可知,使用本发明的养鸭方法对鸭进行喂养,其种鸭的发病率和死亡率为0%,优于对照组;且孵化出的小鸭的发病率和死亡率分别比对照组低37-42%和19-21%。因此,使用本发明的养鸭方法对鸭进行饲喂,可以大大降低鸭病毒性肝炎的发病率和死亡率,从而可以降低养殖户的风险,提高经济效益。
上述说明是针对本发明较佳可行实施例的详细说明,但实施例并非用以限定本发明的专利申请范围,凡本发明所提示的技术精神下所完成的同等变化或修饰变更,均应属于本发明所涵盖专利范围。
Claims (7)
1.一种防治鸭病毒性肝炎的养鸭方法,其特征在于:对于种鸭,用防病鸭饲料进行饲喂,且3次/天,每次让其自由采食至吃饱为止;对于日龄在1-8天以内的小鸭,对鸭皮下注射防病注射液,2天/次,且每次0.4mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.2-0.4g;对于日龄在9-20天以内的小鸭,对鸭皮下注射防病注射液,5天/次,且每次0.3mL,同时用防病鸭饲料进行饲喂,3次/天,且每次0.4-0.5g;对于日龄在20天以上的小鸭,用防病鸭饲料进行饲喂直至出栏,且3次/天,每次让其自由采食至吃饱为止;且每次饲喂后都将鸭子放养到无公害稻田中;
所述防病鸭饲料由能量饲料250-350份、微量元素添加剂1-3份和中草药添加剂8-20份组成;
所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖12-26份、赤芍12-26份、白花蛇舌草12-24份、草决明12-24份、柴胡12-22份、大青叶8-19份、丁葵草8-19份、柏子仁8-17份、茯苓5-16份、甘草5-13份和橘梗3-11份。
2.根据权利要求1所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖17-22份、赤芍17-21份、白花蛇舌草16-19份、草决明15-19份、柴胡14-17份、大青叶11-14份、丁葵草11-13份、柏子仁11-13份、茯苓7-10份、甘草7-9份和橘梗6-9份。
3.根据权利要求1所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,所述中草药添加剂和防病注射液包括以下重量份的原料组分:虎杖18份、赤芍18份、白花蛇舌草17份、草决明17份、柴胡15份、大青叶12份、丁葵草12份、柏子仁12份、茯苓9份、甘草8份和橘梗7份。
4.根据权利要求1所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,所述能量饲料由玉米粉30-55份、绿豆粉24-43份和鱼粉18-27份混合至均匀后,经过粗粉碎过筛80目制成;所述的微量元素添加剂由贝壳粉2-4份和松花粉1-3份混合至均匀制成。
5.根据权利要求1-4任意一项所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,所述防病鸭饲料由以下具体步骤制成:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85-90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液置于真空条件下,经过浓缩并雾化干燥后,粗粉碎并过100目筛,即得到中药粉,备用;
(3)按重量份计,将(2)所得中药粉与能量饲料、微量元素添加剂搅拌至混合均匀,经过低温超微粉碎并过200目筛后,投入制粒机制成颗粒,包装后即可得到成品。
6.根据权利要求5所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,步骤(3)中,所述颗粒的直径为2.8-4.0mm。
7.根据权利要求1-4任意一项所述一种防治鸭病毒性肝炎的养鸭方法,其特征在于,所述防病注射液由以下具体步骤制成:
(1)将各中草药组分虎杖、赤芍、白花蛇舌草、草决明、柴胡、大青叶、丁葵草、柏子仁、茯苓、甘草和橘梗分别净选,然后分别按重量份称取各中草药组分,备用;
(2)将(1)中称取好的各中草药组分混合放入烧杯中,往烧杯中加入体积浓度为85-90%的乙醇至刚好浸没各中草药组分,加热回流,循环浸提3次后过滤,将滤液放入离心机中以2000r/min的速度离心30min,即得中药液,备用;
(3)将(2)中的中药液置于真空条件下,浓缩至含水量为12-16%的稠膏,将所得的稠膏放至室温后,加入体积为6-11%的丙三醇,并加与稠膏和丙三醇相同体积的生理盐水,搅拌至混合均匀,于2℃冷藏静置40小时后过滤,得滤液;所得滤液再依次经0.45μm、0.22μm的微孔滤膜循环过滤2次,经灌封、灭菌后即得本发明1g生药/mL的防病注射液。
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