CN106172094A - A kind of hatching method of acipenser dabryanus fish roe - Google Patents
A kind of hatching method of acipenser dabryanus fish roe Download PDFInfo
- Publication number
- CN106172094A CN106172094A CN201610524944.8A CN201610524944A CN106172094A CN 106172094 A CN106172094 A CN 106172094A CN 201610524944 A CN201610524944 A CN 201610524944A CN 106172094 A CN106172094 A CN 106172094A
- Authority
- CN
- China
- Prior art keywords
- germ cell
- hatching
- fish
- acipenser dabryanus
- radix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 32
- 241000876433 Acipenser dabryanus Species 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000251468 Actinopterygii Species 0.000 claims abstract description 35
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 31
- 210000004602 germ cell Anatomy 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 12
- 238000007654 immersion Methods 0.000 claims abstract description 11
- 230000003068 static effect Effects 0.000 claims abstract 2
- 210000004681 ovum Anatomy 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 claims 1
- 230000004720 fertilization Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 10
- 230000018109 developmental process Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 description 4
- 206010002660 Anoxia Diseases 0.000 description 2
- 241000976983 Anoxia Species 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 230000007953 anoxia Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000883303 Acipenser sinensis Species 0.000 description 1
- 208000007163 Dermatomycoses Diseases 0.000 description 1
- 241000233667 Saprolegnia Species 0.000 description 1
- 241000233658 Saprolegniaceae Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000003929 dermatomycosis Diseases 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides the hatching method of a kind of acipenser dabryanus fish roe, including: in the acipenser dabryanus germ cell Lotic hatching phase to Radix Et Rhizoma Rhei bolt period, use the formalin static soak germ cell 20～40min of 150～250ppm concentration, germ cell is rinsed with clear water after immersion, flowing water is used to continue hatching germ cell again, until fish membrane.The method that the present invention provides both can prevent fish molds short-term large area from occurring, and does not the most affect the normal development of fish roe, and the method is simple.Test result indicate that, the method that the present invention provides can be greatly improved the survival rate of acipenser dabryanus.
Description
Technical field
The present invention relates to artificial propagation techniques field, the hatching method of a kind of acipenser dabryanus fish roe.
Background technology
Acipenser dabryanus, also known as acipenser dabryanus, Sha Lazi, is China's endemic species, within 1988, is listed in country-level focused protection and moves
Thing.The wild resource amount of acipenser dabryanus is the most rare at present, mainly protects this species by artificial propagation.But acipenser dabryanus
During artificial propagation, the especially incubation period of germ cell, it is the tightest that fish molds has become harm acipenser dabryanus development of fertilized ova
The disease of weight, causes loss greatly.
Fish molds is commonly called as white defect, dermatomycosis, and its cause of disease is some kinds of Saprolegniaceae, common are continuous mould, fish molds, silk
The mould grade of capsule belongs to.Fish molds has the strongest environmental suitability and fertility, in fish roe normal incubation pH scope, dissolved oxygen and temperature
Can survive and breeding.When the ovum of hatching causes death because of mechanism damage, anoxia, unfertilized or parasite, and fish molds is with regard to pole
Easily grow.After fish molds occurs, if taken measures not in time, saprolegnia hypha, by mushrooming out and infect the ovum of surrounding, causes
Anoxia, in turn results in large quantities of ovum dead, the most even causes situation about being annihilated.But fish molds is at parasitic initial stage, naked eyes
Can't see symptom；When seen from naked eyes, its mycelia the most deeply grows and spreads diffusion.Therefore, during the artificial propagation of fish,
In the way of finding dead ovum and choosing in time, generally reduce the harm of fish molds.But this mode needs to remove dead ovum in time,
If removing not in time, easily causing fish roe because of fish molds death of luming, being unfavorable for large-scale artificial propagation.
Summary of the invention
In order to effectively reduce the harm of fish molds during acipenser dabryanus artificial propagation, it is contemplated that in providing a kind of Da Shi
The hatching method of Acipenser Sinensis ovum, the method is simple, and can effectively prevention of water mildew short time large area occur.
The invention provides the hatching method of a kind of acipenser dabryanus fish roe, comprise the following steps:
In the acipenser dabryanus germ cell Lotic hatching phase to Radix Et Rhizoma Rhei bolt period, the formalin using 150～250ppm concentration is quiet
Only soak germ cell 20～40min, rinse germ cell with clear water after immersion, then use flowing water to continue hatching germ cell, until fish
Membrane.
Preferably, germ cell 30min is soaked.
Preferably, germ cell is slowly rinsed after immersion with clear water.
The present invention, in the acipenser dabryanus germ cell Lotic hatching phase to Radix Et Rhizoma Rhei bolt period, uses the formaldehyde of 150～250ppm concentration
Solution stand soaks germ cell 20～40min, rinses germ cell with clear water, then use flowing water to continue hatching germ cell after immersion,
Until fish membrane.The method that the present invention provides both can prevent fish molds short-term large area from occurring, and does not the most affect the normal of fish roe and sends out
Educating, the method is simple.Test result indicate that, the method that the present invention provides can be greatly improved the survival rate of acipenser dabryanus.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under not making creative work premise, broadly falls into the model of present invention protection
Enclose.
Embodiment 1
Lotic hatching acipenser dabryanus germ cell, when incubating oosperm to Radix Et Rhizoma Rhei bolt period, soaks by the formalin of 150ppm
30min, slowly rinses with clear water after immersion, then recovers to Lotic hatching, observes incubating oosperm situation ever since, until fish
Membrane, does not all find obvious fish molds caking phenomenon.Observe matched group (being provided without the same batch fish roe that formaldehyde soaks) germ cell
Hatching has certain fish molds caking phenomenon, statistical data to membrane, and matched group survival rate is 37%, and experimental group survival rate is
63%.
Embodiment 2
Lotic hatching acipenser dabryanus germ cell, when incubating oosperm to Radix Et Rhizoma Rhei bolt period, soaks by the formalin of 200ppm
30min, slowly rinses with clear water after immersion, then recovers to Lotic hatching, observes incubating oosperm situation ever since, until fish
Membrane, does not all find obvious fish molds caking phenomenon.Observe matched group (being provided without the same batch fish roe that formaldehyde soaks) germ cell
Hatching has certain fish molds caking phenomenon, statistical data to membrane, and matched group survival rate is 37%, and experimental group survival rate is
54%.
Embodiment 3
Lotic hatching acipenser dabryanus germ cell, when incubating oosperm to Radix Et Rhizoma Rhei bolt period, soaks by the formalin of 250ppm
30min, slowly rinses with clear water after immersion, then recovers to Lotic hatching, observes incubating oosperm situation ever since, until fish
Membrane, does not all find obvious fish molds caking phenomenon.Observe matched group (being provided without the same batch fish roe that formaldehyde soaks) germ cell
Hatching has certain fish molds caking phenomenon, statistical data to membrane, and matched group survival rate is 37%, and experimental group survival rate is
50%.
Comparative example 1
Lotic hatching acipenser dabryanus germ cell, when incubating oosperm to Radix Et Rhizoma Rhei bolt period, soaks by the formalin of 350ppm
30min, slowly rinses with clear water after immersion, then recovers to Lotic hatching, observes incubating oosperm situation ever since, until fish
Membrane, does not all find obvious fish molds caking phenomenon.Observe matched group (being provided without the same batch fish roe that formaldehyde soaks) germ cell
Hatching has certain fish molds caking phenomenon, statistical data to membrane, and matched group survival rate is 37%, and experimental group survival rate is
33%.
Comparative example 2
Lotic hatching acipenser dabryanus germ cell, when incubating oosperm to Radix Et Rhizoma Rhei bolt period, soaks by the formalin of 600ppm
30min, slowly rinses with clear water after immersion, then recovers to Lotic hatching, observes incubating oosperm situation ever since, dense at this
Under degree, germ cell is the most dead.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For Yuan, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a hatching method for acipenser dabryanus fish roe, comprises the following steps:
At acipenser dabryanus germ cell Lotic hatching to Radix Et Rhizoma Rhei bolt period, use the formalin static soak of 150～250ppm concentration
Germ cell 20～40min, rinses germ cell with clear water after immersion, then uses flowing water to continue hatching germ cell, until fish membrane.
Hatching method the most according to claim 1, it is characterised in that Radix Et Rhizoma Rhei bolt soaks germ cell 30min period.
Hatching method the most according to claim 2, it is characterised in that slowly rinse fertilization after a drug immersion with clear water
Ovum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610524944.8A CN106172094A (en) | 2016-07-04 | 2016-07-04 | A kind of hatching method of acipenser dabryanus fish roe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610524944.8A CN106172094A (en) | 2016-07-04 | 2016-07-04 | A kind of hatching method of acipenser dabryanus fish roe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106172094A true CN106172094A (en) | 2016-12-07 |
Family
ID=57464904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610524944.8A Pending CN106172094A (en) | 2016-07-04 | 2016-07-04 | A kind of hatching method of acipenser dabryanus fish roe |
Country Status (1)
| Country | Link |
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| CN (1) | CN106172094A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110250056A (en) * | 2019-07-10 | 2019-09-20 | 淮阴师范学院 | A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate |
| CN112075365A (en) * | 2020-09-25 | 2020-12-15 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving hatching rate of fertilized eggs of Acipenser changjiang |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103712A (en) * | 2007-08-03 | 2008-01-16 | 天津农学院 | Ietalurus punetaus artificial hatching method |
| CN101317558A (en) * | 2008-06-16 | 2008-12-10 | 中国水产科学研究院黑龙江水产研究所 | Distant hybridization method for hucho trout and brachymystax lenok |
| CN101322478A (en) * | 2008-08-01 | 2008-12-17 | 中国水产科学研究院黑龙江水产研究所 | Distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss |
| CN102715115A (en) * | 2012-07-09 | 2012-10-10 | 中国水产科学研究院黑龙江水产研究所 | Phoxinus lagowskii fry breeding method |
-
2016
- 2016-07-04 CN CN201610524944.8A patent/CN106172094A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103712A (en) * | 2007-08-03 | 2008-01-16 | 天津农学院 | Ietalurus punetaus artificial hatching method |
| CN101317558A (en) * | 2008-06-16 | 2008-12-10 | 中国水产科学研究院黑龙江水产研究所 | Distant hybridization method for hucho trout and brachymystax lenok |
| CN101322478A (en) * | 2008-08-01 | 2008-12-17 | 中国水产科学研究院黑龙江水产研究所 | Distant hybridization method of Oncorhynchus masou and Oncorhynchus mykiss |
| CN102715115A (en) * | 2012-07-09 | 2012-10-10 | 中国水产科学研究院黑龙江水产研究所 | Phoxinus lagowskii fry breeding method |
Non-Patent Citations (2)
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| 康志平、董宏伟: "高寒地区匙吻鲟受精卵孵化及仔幼鱼培育技术", 《黑龙江水产》 * |
| 韩骥: "反季节史氏鲟苗种培育技术", 《黑龙江水产》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110250056A (en) * | 2019-07-10 | 2019-09-20 | 淮阴师范学院 | A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate |
| CN110250056B (en) * | 2019-07-10 | 2021-09-24 | 淮阴师范学院 | Method for improving in vitro hatchability of fertilized eggs of macrobrachium nipponense |
| CN112075365A (en) * | 2020-09-25 | 2020-12-15 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving hatching rate of fertilized eggs of Acipenser changjiang |
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Application publication date: 20161207 |