CN106172012A - A kind of Fructus Mori tree group culturation rapid propagating technology - Google Patents
A kind of Fructus Mori tree group culturation rapid propagating technology Download PDFInfo
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- CN106172012A CN106172012A CN201610732232.5A CN201610732232A CN106172012A CN 106172012 A CN106172012 A CN 106172012A CN 201610732232 A CN201610732232 A CN 201610732232A CN 106172012 A CN106172012 A CN 106172012A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of Fructus Mori tree group culturation rapid propagating technology, it is characterised in that use seedling breeding, field-transplanting, field management, flower and fruit management, the prevention and control of plant diseases, pest control and fruit harvesting procedure of processing, use the mode of tissue-culturing rapid propagation to carry out.With drop-proof agent, gibberellins mixed liquor, carbamide, potassium dihydrogen phosphate, boric acid mixed liquor, sterilizing fruit retention.The present invention may select the laterite loess hill mountain planting at ground low altitude area such as China Guangdong, Guangxi, Fujian, can realize nuisanceless, green, the organic production of Fructus Mori tree.Fructus Mori tree whole body is precious, and economic worth is high, and plantation easily, is become a useful person fast, it is easy to popularizing planting.
Description
Technical field
The invention belongs to economy of forestry crop field, particularly a kind of Fructus Mori tree group culturation rapid propagating technology.
Background technology
Fructus Mori tree with Guangdong, Guangxi, Fujian plant as many, development is fast, and fruit tree easily grows, in farmland, hillside fields, loess soda acid
All can plant, and planting technology is less demanding, growth conditions is less demanding, and economic worth is high, leaves sericulture producing natural silk, clothing
Quilt processed, Pupa bombycis alcoholic, can the big benefit of fried cuisines, ripe Mulberry fruit reddish black, sweet and sour taste, good to eat, eliminate indigestion and phlegm, 1 year
Plant to continue and receive every year, be a kind of agricultural economy crop the most suitably greatly developed.
Summary of the invention
It is an object of the invention to according to social development needs, a kind of plantation of research is easily, general the most numerous, hillside grass
Tian Junke plants, and by acquisition process just generation, increases numerous, and root culture, acclimatization and transplants tissue culture goes out well developed root system, and trees are thick
Strong, tree crown body is attractive in appearance, the seedling that greenery grow into forest.
The technical solution of the present invention is such, a kind of Fructus Mori tree group culturation rapid propagating technology, it is characterised in that include with
Lower step:
Step (1) seedling breeding: it is that outer implant material is come by fast reproduction technique that seedling selects with Fructus Mori tree fine individual plant
Test tube Seedling；
Step (2) field-transplanting:
Edaphic condition: select that soil layer is deep, subacidity, well-drained red soil or yellow earth hilly upland；
Atmospheric condition: air is the most pollution-free, air index II is 0.5；
Illumination condition: select the opening below height above sea level 600m illumination is sufficient to plant；
4. condition of water quality: annual rainfall at 1600mm, rain heat same period, has the water source and facility being available for irrigating；
5. line-spacing specification: carry out digging cave by seeding row spacing 0.8m, the most appropriate farm manure is made base manure, is covered fine earth and pass the winter, at rain in spring
Rear fine day or cloudy day transplant；
Step (3) field management:
Intertillage: carry out middle weeding after transplanting every year；
Water and fertilizer management: use radial canal to excute a law by N:P after field planting2O5:K2O=0.8:0.3:0.6 joins and executes azophoska, the best fruiting period
By N:P2O5:K2O=0.8:0.3:0.6=1.2:0.5:1.0 joins and executes azophoska；
Irrigation and drainage manage: trophophase and fruit expanding period should keep the field capacity of 70%～82% so that ground moistening, and bud divides
The change phase keeps the field capacity of 55%, reduces water supply and be beneficial to fruit tree and timely enter rest period after late fall；
4. management is pruned: treelet expands shrubbery to cultivate and is shaped as main, thin and delicate with remove in strain to becoming tree in age to be then mainly
Branch, old and feeble branch, sick branch, overstocked branch and the old branch that bounces back are main；
Step (4) flower and fruit management
Pollination: honeybee is put in employing and artificial point is awarded and feather duster rolls and pollinates；
Fruit retention: young fruit period sprays drop-proof agent, fruit expanding period sprays gibberellins mixed liquor and carries out fruit retention；
Step (5) prevention and control of plant diseases, pest control: main pest and disease damage mainly have anthrax, fruit rot, dark mildew, aphid, aleyrodid, Gryllus Chinensis and
Birds, to put prevention first, carry out integrated control with biological pesticide；
Step (6) fruit harvesting:
Fair weather is selected to gather in batches in atropurpureus ripe Fructus Mori tree fruit;
Leaves of gathering feeds silkworm, gathers every day once, and after gathering, water spray drenches once；
Step (7). the seedling breeding described in step (1) is that the mode using tissue-culturing rapid propagation is carried out, and it mainly comprises the following steps:
The collection of outer implant and process: select Fructus Mori tree fine individual plant gives birth to the full branch of internal organs bud, then in detergent after defoliation
Water soaking 16min, after outwash, tap water rinses 1.5h, is cut into the stem section of long 3.5cm band axillalry bud, first with after 75% ethanol disinfection 6s
With aseptic washing 2 times, then with 0.1% mercuric chloride solution sterilization 25min, with standby after aseptic water washing 4 times；
Initial culture: above-mentioned stem section is inoculated on initial culture base, first full light culture 42 days under the conditions of 26 DEG C, then
Illumination every day 8 hours, intensity of illumination is 2500lx, until induced synthesis adventitious bud, described initial culture base is: MS+3-
BA1.0mg/L+NAA0.5mg/L+CPPU0.06 mg/L+ Ad1.2mg/L+ sucrose 15g/L+ agar 5g/L, pH is 5.5；
Enrichment culture: above-mentioned adventitious bud proceeds to carry out on proliferated culture medium enrichment culture, after inoculation first under the conditions of 26 DEG C
Full light culture 12 days, then illumination every day 12 hours, intensity of illumination is 4500lx, and cultivation temperature is cultivated under conditions of being 26 DEG C,
Once, described proliferated culture medium is: MS+5-BA1.0mg/L+NAA0.5mg/L+IBA0.3mg/L+ in switching in 44 days
Ad1.2mg/L+ sucrose 18g/L+ agar 6g/L, pH is 5.5；
4. root culture: cut to be inoculated on root media by the Multiple Buds of above-mentioned height about 2cm and carry out root culture, connects
First full light culture 8 days, then illumination every day 16 hours under the conditions of 26 DEG C after Zhong, intensity of illumination is 4500lx, and cultivation temperature is
Cultivating under conditions of 26 DEG C to taking root, described root media is: 1/2MS+IBA0.5mg/L+GGR1.5mg/L+PG12mg/
L+ sucrose 20g/L+ agar 3g/L, pH is 5.5；
5. acclimatization and transplants: after going bottle cap to be placed in natural lighting lower refining seedling 8 days the rooting tube plantlet of high about 12cm, by test tube Seedling from
Culture bottle takes out, washes root culture medium off, plant in the substrate being mixed into by peat soil and yellow sand mud and be colonizated in big Tanaka.
Drop-proof agent described in step (4) is 75% drop-proof agent that 1.5g is watered 120kg.
Gibberellins mixed liquor described in step (5) is 0.006% gibberellins+0.2% carbamide+0.2% potassium dihydrogen phosphate+0.06%
Boric acid mixed liquor.
Gathering described in step (6) is:Mulberry fruit of gathering directly eats；Folium Mori of gathering feed silkworm；It it is Fruit
Receive clean, sterilization, air-dry after steaming, be vacuum-packed.
Present invention advantage compared with prior art is: technical matters is advanced, and method easily supports and holds, field-transplanting, and field is managed
Reason easily, facilitates feasible, and labor intensity is the highest, can plant to the girth of a garment, the anti-evil of insect protected, simple possible, gathers conveniently.
Detailed description of the invention
Following example are to further illustrate the present invention, are not limitations of the present invention.
(1) seedling breeding: it is that outer implant material is come by fast reproduction technique that seedling selects with Fructus Mori tree fine individual plant
Test tube Seedling.Its process is as follows: the collection of outer implant and process: select Fructus Mori tree fine individual plant gives birth to the full branch of internal organs bud then,
In detergent water soaking 16min after defoliation, after outwash, tap water rinses 1.5h, is cut into the stem section of long 0.8cm band axillalry bud, first uses
With aseptic washing 2 times after 75% ethanol disinfection 9s, then with 0.1% mercuric chloride solution sterilization 15min, with standby after aseptic water washing 4 times.
Initial culture: above-mentioned stem section is inoculated on initial culture base, first full light culture 45 days, then every day under the conditions of 26 DEG C
Illumination 8 hours, intensity of illumination is 2500lx, until induced synthesis adventitious bud.Described initial culture base is: MS+5-
BA1.0mg/L+NAA0.5mg/L+CPPU0.06mg/L+ Ad1.2mg/L+ sucrose 20g/L+ agar 5g/L, pH is 5.5.Propagation
Cultivate: above-mentioned adventitious bud proceeds to carry out on proliferated culture medium enrichment culture, first full light culture under the conditions of 26 DEG C after inoculation
10 days, then illumination every day 12 hours, intensity of illumination was 5000lx, and cultivation temperature is cultivated under conditions of being 26 DEG C, switching in 52 days
Once.Described proliferated culture medium is: MS+5-BA1.0mg/L+NAA0.5mg/L+IBA0.3mg/L+ Ad1.2mg/L+ sucrose
15g/L+ agar 3g/L, pH are 5.5.4. root culture: the Multiple Buds of above-mentioned height about 6cm is cut and is inoculated into root culture
Root culture is carried out, first full light culture 8 days, then illumination every day 15 hours, intensity of illumination under the conditions of 26 DEG C after inoculation on base
For 5500lx, cultivation temperature is cultivated to taking root under conditions of being 28 DEG C.Described root media is: 1/2MS+IBA0.8mg/L
+ GGR1.2mg/L+PG0.8mg/L+ sucrose 20g/L+ agar 5g/L, pH is 5.5.5. acclimatization and transplants: by taking root of high about 12cm
Test tube Seedling goes bottle cap natural lighting lower refining seedling after 8 days, is taken out by test tube Seedling, wash root culture medium off from culture bottle, plant into by
In the substrate that peat soil and yellow sand mud are mixed into and be colonizated in big Tanaka, transplanting survival rate more than 95%.
(2) field-transplanting:
Edaphic condition: select that soil layer is deep, subacidity, well-drained Guangdong, Guangxi, Fujian plantation red soil or yellow earth hills
Hillside fields；
Atmospheric condition: air is the most pollution-free, air index II is between 0.5；
Illumination condition: select the opening below height above sea level 600m illumination is sufficient to plant；
4. condition of water quality: annual rainfall, between 1600mm, rain heat same period, has the water source and facility being available for irrigating；
5. line-spacing specification: carry out digging cave by seeding row spacing 0.8m × 1.0m, base manure made by farm manure the most in right amount, covers fine earth and passes the winter,
After spring rain, fine day or cloudy day transplant；
(3) field management
Intertillage: carry out middle weeding after transplanting every year；
Water and fertilizer management: use radial canal to excute a law by N:P after field planting2O5:K2O=08:0.5:0.60 joins and executes azophoska, the best fruiting period
By N:P2O5:K2O=0.8:0.5:0.60=1.2:0.5:1.0 joins and executes azophoska.
Irrigation and drainage manage: trophophase and fruit expanding period should keep the field capacity of 85% so that ground moistening.Bud divides
The change phase keeps the field capacity of 65%, reduces water supply and be beneficial to fruit tree and timely enter rest period after late fall.
4. management is pruned: treelet expands shrubbery to cultivate and is shaped as main, is then mainly to remove in strain becoming tree in age
Thin and delicate branch, old and feeble branch, sick branch, overstocked branch and the old branch that bounces back are main.
(4) flower and fruit management
Pollination: honeybee is put in employing and artificial point is awarded and feather duster rolls and pollinates；
Fruit retention: young fruit period sprays 2g and is watered 75% drop-proof agent of 90kg, fruit expanding period sprays 0.006% gibberellins+0.8% carbamide
+ 0.8% potassium dihydrogen phosphate+0.06% boric acid mixed liquor carries out fruit retention.
(5) prevention and control of plant diseases, pest control: main pest and disease damage mainly have anthrax, fruit rot, dark mildew, aphid, aleyrodid, Gryllus Chinensis and
Birds etc., to put prevention first, carry out integrated control with biological pesticide.
(6) fruit harvesting and storage:Fruit harvesting, selects fair weather to gather in batches in atropurpureus ripe Fructus Mori
Fruit, fruit stores in dry Tibetan mode,Folium Mori feed silkworm, breed silkworms, pluck every day once, and after having plucked, water spray is once.Really
Real processing employing technique is: fruit harvesting → cleaning → airing → gas steams → vacuum packaging.
Claims (5)
1. a Fructus Mori tree group culturation rapid propagating technology, it is characterised in that comprise the following steps:
Step (1) seedling breeding: it is that outer implant material is come by fast reproduction technique that seedling selects with Fructus Mori tree fine individual plant
Test tube Seedling；
Step (2) field-transplanting:
Edaphic condition: select that soil layer is deep, subacidity, well-drained red soil or yellow earth hilly upland；
Atmospheric condition: air is the most pollution-free, air index II is 0.5；
Illumination condition: select the opening below height above sea level 600m illumination is sufficient to plant；
4. condition of water quality: annual rainfall at 1600mm, rain heat same period, has the water source and facility being available for irrigating；
5. line-spacing specification: carry out digging cave by seeding row spacing 0.8m, the most appropriate farm manure is made base manure, is covered fine earth and pass the winter, at rain in spring
Rear fine day or cloudy day transplant；
Step (3) field management:
Intertillage: carry out middle weeding after transplanting every year；
Water and fertilizer management: use radial canal to excute a law by N:P after field planting2O5:K2O=0.8:0.3:0.6 joins and executes azophoska, the best fruiting period
By N:P2O5:K2O=0.8:0.3:0.6=1.2:0.5:1.0 joins and executes azophoska；
Irrigation and drainage manage: trophophase and fruit expanding period should keep the field capacity of 70%～82% so that ground moistening, and bud divides
The change phase keeps the field capacity of 55%, reduces water supply and be beneficial to fruit tree and timely enter rest period after late fall；
4. management is pruned: treelet expands shrubbery to cultivate and is shaped as main, thin and delicate with remove in strain to becoming tree in age to be then mainly
Branch, old and feeble branch, sick branch, overstocked branch and the old branch that bounces back are main；
Step (4) flower and fruit management
Pollination: honeybee is put in employing and artificial point is awarded and feather duster rolls and pollinates；
Fruit retention: young fruit period sprays drop-proof agent, fruit expanding period sprays gibberellins mixed liquor and carries out fruit retention；
Step (5) prevention and control of plant diseases, pest control: main pest and disease damage mainly have anthrax, fruit rot, dark mildew, aphid, aleyrodid, Gryllus Chinensis and
Birds, to put prevention first, carry out integrated control with biological pesticide；
Step (6) fruit harvesting:
Fair weather is selected to gather in batches in atropurpureus ripe Fructus Mori tree fruit;
Leaves of gathering feeds silkworm, gathers every day once, and after gathering, water spray drenches once.
A kind of Fructus Mori tree group culturation rapid propagating technology the most according to claim 1, it is characterised in that the seedling described in step (1) is numerous
Educating the mode being to use tissue-culturing rapid propagation to carry out, its major way is:
The collection of the outer implant of mode and process: select Fructus Mori tree fine individual plant gives birth to the full branch of internal organs bud, then in washing after defoliation
Clothing powder water soaking 16min, after outwash, tap water rinses 1.5h, is cut into the stem section of long 3.5cm band axillalry bud, first uses 75% ethanol disinfection
With aseptic washing 2 times after 6s, then with 0.1% mercuric chloride solution sterilization 25min, with standby after aseptic water washing 4 times；
Mode initial culture: above-mentioned stem section is inoculated on initial culture base, first full light culture 42 days under the conditions of 26 DEG C,
Then illumination every day 8 hours, intensity of illumination is 2500lx, until induced synthesis adventitious bud, described initial culture base is: MS+
3-BA1.0mg/L+NAA0.5mg/L+CPPU0.06 mg/L+ Ad1.2mg/L+ sucrose 15g/L+ agar 5g/L, pH is 5.5；
Mode enrichment culture: above-mentioned adventitious bud proceeds to carry out on proliferated culture medium enrichment culture, first at 26 DEG C of bars after inoculation
Full light culture 12 days under part, then illumination every day 12 hours, intensity of illumination is 4500lx, and cultivation temperature is trained under conditions of being 26 DEG C
Supporting, once, described proliferated culture medium is: MS+5-BA1.0mg/L+NAA0.5mg/L+IBA0.3mg/L+ in switching in 44 days
Ad1.2mg/L+ sucrose 18g/L+ agar 6g/L, pH is 5.5；
Mode 4. root culture: cut to be inoculated on root media by the Multiple Buds of above-mentioned height about 2cm and carry out training of taking root
Supporting, first full light culture 8 days, then illumination every day 16 hours under the conditions of 26 DEG C after inoculation, intensity of illumination is 4500lx, cultivates temperature
Degree is cultivated to taking root under conditions of being 26 DEG C, and described root media is: 1/2MS+IBA0.5mg/L+GGR1.5mg/L+
PG12mg/L+ sucrose 20g/L+ agar 3g/L, pH is 5.5；
Mode 5. acclimatization and transplants: after going bottle cap to be placed in natural lighting lower refining seedling 8 days the rooting tube plantlet of high about 12cm, by test tube
Seedling takes out from culture bottle, washes root culture medium off, plants in the substrate being mixed into by peat soil and yellow sand mud and be colonizated in big
Tanaka.
A kind of Fructus Mori tree group culturation rapid propagating technology the most according to claim 1, it is characterised in that anti-fall described in step (4)
Element is watered 75% drop-proof agent of 120kg for 1.5g.
A kind of Fructus Mori tree group culturation rapid propagating technology the most according to claim 1, it is characterised in that the gibberellins described in step (4)
Mixed liquor is 0.006% gibberellins+0.2% carbamide+0.2% potassium dihydrogen phosphate+0.06% boric acid mixed liquor.
A kind of Fructus Mori tree group culturation rapid propagating technology the most according to claim 1, it is characterised in that gathering described in step (6)
It is:Mulberry fruit of gathering directly eats；Folium Mori of gathering feed silkworm；It is that fruit harvesting is cleaned, sterilized, air-dried, vacuum packet after steaming
Dress.
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| CN201610732232.5A CN106172012A (en) | 2016-08-26 | 2016-08-26 | A kind of Fructus Mori tree group culturation rapid propagating technology |
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| CN201610732232.5A CN106172012A (en) | 2016-08-26 | 2016-08-26 | A kind of Fructus Mori tree group culturation rapid propagating technology |
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| CN106172012A true CN106172012A (en) | 2016-12-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108157051A (en) * | 2018-02-02 | 2018-06-15 | 广西壮歌农业科技桑博园有限责任公司 | A kind of implantation methods of mulberry tree |
| CN108901571A (en) * | 2018-08-05 | 2018-11-30 | 贵港市德源贸易有限公司 | A kind of implantation methods of hill gooseberry |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108157051A (en) * | 2018-02-02 | 2018-06-15 | 广西壮歌农业科技桑博园有限责任公司 | A kind of implantation methods of mulberry tree |
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Application publication date: 20161207 |
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