CN106171996B - A kind of rapid propagation method of wild white birch - Google Patents
A kind of rapid propagation method of wild white birch Download PDFInfo
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- CN106171996B CN106171996B CN201610576291.8A CN201610576291A CN106171996B CN 106171996 B CN106171996 B CN 106171996B CN 201610576291 A CN201610576291 A CN 201610576291A CN 106171996 B CN106171996 B CN 106171996B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The present invention provides a kind of rapid propagation method of wild white birch, comprises the following steps：1) acquisition of wild white birch stem section explant；2) Fiber differentiation of wild white birch stem section explant；3) Multiplying culture of wild white birch Multiple Buds；4) culture of rootage of wild white birch：It is divided into 2 stages, first stage：First by the simple bud of Multiple Buds cutting in the first root media culture, 2800~3000Lux is placed in, daily illumination 20h cultures are until 8~10d of taking root；Second stage：Transfer again and 20~30d is cultivated in the second root media, grow good root system；5) hardening of wild white birch tissue-cultured seedling and transplanting：After 7~10d of normal temperature hardening, 30~35d of culture in matrix is transplanted into, obtains the quick breeding seedling of wild white birch；6) transplant, be transplanted to planting site.The present invention solves the problems, such as wild white birch tissue cultures to take root, and difficult, rooting rate is low.
Description
Technical field
The present invention relates to a kind of rapid propagation method of wild white birch.
Background technology
White birch (Bettlla platyphylla suk), belongs to Betulaceae birch platymiscium, and deciduous tree is high up to 20-
25m, diameter of a cross-section of a tree trunk 1.3 meters above the ground 50cm, bark white, smoothly, paper-like layering are peeled off；The brown bronzing of spray, leaf triangular shape is avette, and tip is tapering, base
Portion's section shape, pyrene is flat, and both sides film quality wing, the 5-6 months bloom, and August fruit is ripe.
White birch originates in Daxing'an Mountainrange, the Xiaoxinanlin Mountains, Changbai Mountain and the A Er of North China alpine region and northwest of Northeast China
Mount Taishan；Be distributed in China northeast Xing'anling mountains, Changbai Mountain, Shanxi, Hunan, the Hubei in North China, Inner Mongol of northwest, Ningxia,
Shaanxi, Qinghai, Tibet, the Sichuan in southwest, northwestern Yunnan Province.The hygrophilous profit rich soil of white birch light, resistance to dry lean, growth is rapid,
Short life, rudiment power is strong, is that one of main vanguard tree seed of scondary forest is formed after forest deterioration, and temperate zone broad-leaf forest and broad-leaved
The chief species of mixed forest.White birch texture is straight, and structure is thin, is good sawing raw material and disposable chopsticks processing in hardness
Raw material, pulpwood, glued material, construction timber and general civilian material can be also used as, bark can extract white birch oil, and white birch juice can be opened
Beverage is sent out, leaf is also pharmaceutically acceptable, and nectariferous plant.
White birch branch it is fine and soft it is sagging, posture is graceful, smooth pure white, as shade tree and Garden virescence tree, it is unique to have
Ornamental plantation is worth.Shortcut of the wild white birch breeding as a fast-developing greening is improved, is more come in urban landscaping
More favored by people, improve urban green space landscape in a short time, improve ecological benefits, the optimization greenery patches knot of urban green space
Structure, formation etc. in advance of urban ecological environment is promoted to play a positive role.At present, white birch has been listed in national two level
Protect the plant protection resource of plant and emphasis, moreover, white birch mainly by seminal propagation and reproduction speed it is slow, it is difficult to meet city
Field demand.Therefore, by being studied the quick breeding of white birch to expand stock number, can solve imbalance between supply and demand.
The content of the invention
In order to solve the above problems, the present invention provides a kind of rapid propagation method of wild white birch, at the technology of the present invention
Wild white birch breeding to be managed, wild white birch reproductive survival rate can be improved, survival rate can reach more than 98%, and with process letter
Singly, the characteristics of chemical raw material species and dosage are few, processing time is short and cost are low.
The technical solution adopted by the present invention is：
A kind of rapid propagation method of wild white birch, comprises the following steps：
1) acquisition of wild white birch stem section explant：Stem section of the 6cm length containing bud is intercepted from wild white birch bearing tree is
Explant, explant is carried out disinfection after processing, aseptic water washing, suck dry moisture, branch section is cut into the stem section of one axillary bud of band；
2) Fiber differentiation of wild white birch stem section explant：Obtained explant is subjected to Fiber differentiation, is 25 in temperature
~28 DEG C, intensity of illumination is 2200~2500Lux, and axillary bud starts to sprout after daily 14~16h of illumination, 40~45d, is formed small
Bud；
3) Multiplying culture of wild white birch Multiple Buds：The new long axillary bud seedling turned out aseptically is cut into a band one
The stem section of individual axillary bud, access proliferated culture medium temperature be 28~30 DEG C culture, be placed in the daily illumination 18 of 2600~2800Lux~
20h forms Multiple Buds after cultivating 25~30d；
4) culture of rootage of wild white birch：It is divided into 2 stages, first stage：First by the simple bud of Multiple Buds cutting first
Root media culture, 2800~3000Lux is placed in, daily illumination 20h cultures are until 8~10d of taking root；Second stage：Turn again
20~30d of culture in the second root media is met, grows good root system；
5) hardening of wild white birch tissue-cultured seedling and transplanting：After 7~10d of normal temperature hardening, be transplanted into matrix culture 30~
35d, obtain the quick breeding seedling of wild white birch；
6) transplant, be transplanted to planting site.
A kind of rapid propagation method of described wild white birch, Fiber differentiation based component is in step 2) Fiber differentiation：WPM
+ 6-BA 1.5~2.0mg/L+NAA, 1.0~2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH5.5~6.5.
A kind of rapid propagation method of described wild white birch, the medium component of proliferated culture medium is in step 3)：MS+
NAA 1.0~2.0mg/L+IBA, 0.05~0.2mg/L+ vitamin Bs61.5~2.0mg/L+GA31.5~2.0mg/L+ sucrose
40g/L+ agar 5g/L, pH 5.5~6.5.
A kind of rapid propagation method of described wild white birch, the first culture of rootage based component is described in step 4)：1/
4MS+IBA 1.0~1.5mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~2.0mg/L+ sucrose 35g/L+ agar
5g/L, pH 5.5~6.5.
A kind of rapid propagation method of described wild white birch, the second culture of rootage based component is described in step 4)：1/
2MS+NAA 0.5~1.0mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~2.0mg/L+GA31.5~2.0mg/L+
Sucrose 35g/L+ agar 5g/L, pH 5.5~6.5.
A kind of rapid propagation method of described wild white birch, step 6) transplanting, it is specially：When seedling lateral root number reaches
8~10, when main root length reaches 15cm, start transplanting to the big Tanaka of 60 × 60cm line spaces or plant into height × a diameter of 30
In × 35cm container, 90~100cm of height container seedling is trained, is transplanted to planting site.
The invention has the advantages that：
1. the culture medium that Fiber differentiation, Multiplying culture, culture of rootage are prepared in the present invention, the seedling of acquisition take root easily,
Healthy and strong degeneration-resistant, resistant to diseases and insects is strong, and seedling neat and consistent, and quality is high, and cultivation management is easier to.The group obtained using the present invention
Seedling is trained, adaptable to urban environment, solving the problems, such as wild white birch tissue cultures to take root, difficult, rooting rate is low.
2. the material that the wild white birch of processing uses is simple.
3. high treating effect：Wild white birch survival rate can reach more than 98% after processing.
4. cost is low：Whole processing technology only needs several frequently seen chemicals.
5. recruitment is few：Handling wild white birch only needs 3-5 worker.
6. technique is simple, is easy to grasp.
7. it is easy to mass production：Because cost is low, technique is simple, it is easy to mass production.
8. pollution is lighter：The remaining decoction of wild white birch per treatment can add the use of medicine Posterior circle.
9. provide advanced technical support for the wild white birch breeding in areas of Shenyang.
Embodiment
Embodiment 1, a kind of rapid propagation method of wild white birch
(1) method is as follows：
1) acquisition of wild white birch stem section explant：
From wild white birch bearing tree intercept 6cm length the stem section containing bud be explant, by explant carry out disinfection processing,
After aseptic water washing, suck dry moisture, branch section is cut into the stem section of one axillary bud of band；
2) Fiber differentiation of wild white birch stem section explant：
Obtained explant is subjected to Fiber differentiation, is 25~28 DEG C in temperature, intensity of illumination is 2200~2500Lux,
Axillary bud starts to sprout after daily illumination 14~16h, 40~45d, forms budlet；The Fiber differentiation based component is：WPM+6-BA
1.5~2.0mg/L+NAA, 1.0~2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH5.5~6.5；
3) Multiplying culture of wild white birch Multiple Buds：
The new long axillary bud seedling turned out aseptically is cut into the stem section of one axillary bud of a band, accesses proliferated culture medium
It is 28~30 DEG C of cultures in temperature, is placed in the daily 18~20h of illumination of 2600~2800Lux, is formed and grown thickly after 25~30d of culture
Bud；The Multiplying culture based component is：MS+NAA 1.0~2.0mg/L+IBA, 0.05~0.2mg/L+ vitamin Bs61.5~
2.0mg/L+GA31.5~2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH 5.5~6.5；
4) culture of rootage of wild white birch：2 stages are divided into,
First stage：The simple bud of Multiple Buds cutting is first placed in 2800~3000Lux in the first root media culture,
Daily illumination 20h cultures are until 8~10d of taking root；
Second stage：Transfer again and 20~30d is cultivated in the second root media, grow good root system；First life
Root medium component is：1/4MS+IBA 1.0~1.5mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~2.0mg/L
+ sucrose 35g/L+ agar 5g/L, pH 5.5~6.5；The second culture of rootage based component is：1/2MS+NAA 0.5~
1.0mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~2.0mg/L+GA31.5~2.0mg/L+ sucrose 35g/L+ fine jades
Fat 5g/L, pH 5.5~6.5；
5) hardening of wild white birch tissue-cultured seedling and transplanting：
After 7~10d of normal temperature hardening, 30~35d of culture in matrix is transplanted into, obtains the quick breeding seedling of wild white birch；
6) transplant
When seedling lateral root number reaches 8~10, when main root length reaches 15cm, start transplanting to 60 × 60cm line spaces
Big Tanaka or plant in high × a diameter of 30 × 35cm container, be trained 90~100cm of height container seedling, be transplanted to kind
Plant ground.
The contrast experiment of embodiment 2
1st, material selection：
It is explant that stem section of the 6cm length containing bud is intercepted from the wild white birch bearing tree of 8~10 years, and explant is carried out
Disinfect, after aseptic water washing, suck dry moisture, branch section is cut into the stem section 80 of one axillary bud of band, is randomly divided into 4 groups.
Group 1：Using the method for embodiment 1.
Group 2：Identical with the method for embodiment 1, difference is：In the 2) step, the wild white birch of the present invention is not used
Rapid propagation method, and use MS+6-BA 0.5~1.0mg/L+NAA, 0.5~1.0mg/L+ sucrose 40g/L+ agar 5g/
L, pH5.5~6.5；
Group 3：Identical with the method for embodiment 1, difference is：In the 3) step, the wild white birch of the present invention is not used
Rapid propagation method, and use MS+NAA1.0~2.0mg/L+IBA0.05~0.2mg/L+ sucrose 40g/L+ agar 5g/L,
PH5.5~6.5；
Group 4：Identical with the method for embodiment 1, difference is：In the 4) step, the wild white birch of the present invention is not used
Rapid propagation method described in the first culture of rootage based component, and use：1/4MS+IBA 1.0~1.5mg/L+ sucrose 35g/L+
Agar 5g/L, pH 5.5~6.5；
Group 5：Identical with the method for embodiment 1, difference is：In the 4) step, the wild white birch of the present invention is not used
Rapid propagation method described in the second culture of rootage based component, and use：0.5~1.0mg/L+ of 1/2MS+NAA fishbone dusts 2.0
~2.5g/L+ vitamin Bs11.5~2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5~6.5；
2nd, result
The transplanting in March of First Year, second and observation in the June growing state of the 3rd year, as a result such as table 1.
Table 1
From table 1, survival rate can averagely 1. be improved up to 98% using the wild white birch reproductive survival rate of method of the present invention
Up to more than 70%.Complete to provide guarantee for the high standard of Landscaping of Northern City, high quality.2. the raising of the present invention is wild
The quick breeding of white birch, the well developed root system of nursery stock can be made, lateral root number is more, and volume increase income is big.3. the raising wild white of the present invention
The rapid propagation method Water-saving effect of birch is good, raw material is easy to get, small investment, quick, water saving saving of labor's application.
Claims (5)
1. a kind of rapid propagation method of wild white birch, it is characterised in that comprise the following steps：
1) acquisition of wild white birch stem section explant：It is explant that stem section of the 6cm length containing bud is intercepted from wild white birch bearing tree
Body, explant is carried out disinfection after processing, aseptic water washing, suck dry moisture, branch section is cut into the stem section of one axillary bud of band；
2) Fiber differentiation of wild white birch stem section explant：Obtained explant is subjected to Fiber differentiation, is 25~28 in temperature
DEG C, intensity of illumination is 2200~2500Lux, and axillary bud starts to sprout after daily 14~16h of illumination, 40~45d, forms budlet；
3) Multiplying culture of wild white birch Multiple Buds：The new long axillary bud seedling turned out aseptically is cut into one armpit of a band
The stem section of bud, access proliferated culture medium are 28~30 DEG C of cultures in temperature, are placed in the daily 18~20h of illumination of 2600~2800Lux
Multiple Buds are formed after 25~30d of culture；The medium component of proliferated culture medium is：1.0~2.0mg/L+IBA of MS+NAA 0.05
~0.2mg/L+ vitamin Bs61.5~2.0mg/L+GA31.5~2.0mg/L+ sucrose 40g/L+ agar 5g/L, pH 5.5~
6.5；
4) culture of rootage of wild white birch：It is divided into 2 stages, first stage：First the simple bud that Multiple Buds are cut is taken root first
Medium culture, 2800~3000Lux is placed in, daily illumination 20h cultures are until 8~10d of taking root；Second stage：Transfer again
20~30d is cultivated in two root medias, grows good root system；
5) hardening of wild white birch tissue-cultured seedling and transplanting：After 7~10d of normal temperature hardening, 30~35d of culture in matrix is transplanted into, is obtained
Obtain the quick breeding seedling of wild white birch；
6) transplant, be transplanted to planting site.
2. a kind of rapid propagation method of wild white birch as claimed in claim 1, it is characterised in that in step 2) Fiber differentiation
Fiber differentiation based component is：WPM+6-BA 1.5~2.0mg/L+NAA, 1.0~2.0mg/L+ sucrose 40g/L+ agar 5g/L,
PH5.5~6.5.
3. a kind of rapid propagation method of wild white birch as claimed in claim 1, it is characterised in that first described in step 4)
Culture of rootage based component is：1/4MS+IBA 1.0~1.5mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~
2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5~6.5.
4. a kind of rapid propagation method of wild white birch as claimed in claim 1, it is characterised in that second described in step 4)
Culture of rootage based component is：1/2MS+NAA 0.5~1.0mg/L+ fishbone dust 2.0~2.5g/L+ vitamin Bs11.5~
2.0mg/L+GA31.5~2.0mg/L+ sucrose 35g/L+ agar 5g/L, pH 5.5~6.5.
5. a kind of rapid propagation method of wild white birch as claimed in claim 1, it is characterised in that step 6) is transplanted, specifically
For：When seedling lateral root number reaches 8~10, when main root length reaches 15cm, it is big to 60 × 60cm line spaces to start transplanting
Tanaka is planted in high × a diameter of 30 × 35cm container, is trained 90~100cm of height container seedling, is transplanted to plantation
Ground.
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| CN108633737A (en) * | 2018-05-15 | 2018-10-12 | 句容市茂润苗木有限公司 | A kind of method for culturing seedlings of cherry tree |
| CN108703071A (en) * | 2018-05-18 | 2018-10-26 | 句容市茂润苗木有限公司 | A kind of white poplar fast seedling-cultivating method |
| CN112189564A (en) * | 2020-11-03 | 2021-01-08 | 毕节市林业科学研究所 | Method for propagating betula luminifera aseptic seedlings by tissue culture technology |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| RU2009143124A (en) * | 2009-11-24 | 2011-05-27 | Российская Федерация в лице Федерального агентства по науке и инновациям (RU) | BINARY VECTOR pBI-4CL, BINARY VECTOR pBI-Xeg, BINARY VECTOR pGS, METHOD FOR PRODUCING TRANSGENIC PLANTS CONTAINING A REDUCED NUMBER OF LIGNIN, GREEN CRANBERRY |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN105684904A (en) * | 2016-02-03 | 2016-06-22 | 东北林业大学 | Method for preparing haploid regenerated plants from betulaplatyphylla anther by virtue of isolated culture |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP3318037B2 (en) * | 1993-04-20 | 2002-08-26 | 住友林業株式会社 | Mass growth method of birch |
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2016
- 2016-07-21 CN CN201610576291.8A patent/CN106171996B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| RU2009143124A (en) * | 2009-11-24 | 2011-05-27 | Российская Федерация в лице Федерального агентства по науке и инновациям (RU) | BINARY VECTOR pBI-4CL, BINARY VECTOR pBI-Xeg, BINARY VECTOR pGS, METHOD FOR PRODUCING TRANSGENIC PLANTS CONTAINING A REDUCED NUMBER OF LIGNIN, GREEN CRANBERRY |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN105684904A (en) * | 2016-02-03 | 2016-06-22 | 东北林业大学 | Method for preparing haploid regenerated plants from betulaplatyphylla anther by virtue of isolated culture |
Non-Patent Citations (1)
| Title |
|---|
| "白桦组织培养技术研究";张学英等;《北方园艺》;20081231(第8期);第176-178页尤其是摘要、第1.1-1.2.3以及第2.5节 * |
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