CN101502237A - Micro-propagation method for white birch with purple leaf - Google Patents
Micro-propagation method for white birch with purple leaf Download PDFInfo
- Publication number
- CN101502237A CN101502237A CNA2009100713455A CN200910071345A CN101502237A CN 101502237 A CN101502237 A CN 101502237A CN A2009100713455 A CNA2009100713455 A CN A2009100713455A CN 200910071345 A CN200910071345 A CN 200910071345A CN 101502237 A CN101502237 A CN 101502237A
- Authority
- CN
- China
- Prior art keywords
- illumination
- micro
- white birch
- purple leaf
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a micropropagation method of purple-leaf birch, and relates to a micropropagation method. The invention settles the problems of long breeding cycle, long propagation efficiency, high cost and low survival rate. The method of the invention comprises the following steps: 1. sterilizing the explant; 2. cutting the explant to stem segment, and then executing induction cultivation for obtaining micro-branch; 3. cutting the micro-branch to stem segment and proliferating in the culture medium; and 4. executing invigoration culture to the proliferated micro-branch, sticking into a rooting substrate for executing rooting culture for finishing the micropropagation of purple-leaf birch. The micropropagation method of purple-leaf birch of the invention has the advantages of short propagation period, high efficiency and excellent genetic stability. The propagation efficiency is that the purple-leaf birch propagates for 30-40 times every 30-40 days. The method of the invention also has the advantages of simple operating technique, labor saving, time saving and low cost. The cost is saved by 30%-50% compared with the prior art. Furthermore the rooting rate and survival rate of seedling propagation equally can obtain 100%.
Description
Technical field
The present invention relates to a kind of micro-propagation method.
Background technology
White birch with purple leaf (Betulapo pulifolia x Whitespire ' Royal Frost ') is drooping birch and Asia white birch through the hybridization repeatedly and the new varieties of coming out according to leaf color characteristic long-term breeding, English " BetulaRoyal Frost Penci-2 " by name.White birch with purple leaf is the suitable fine tree species of viewing and admiring throughout the year, its blade remains the wine redness in whole spring and summer, then become yellow, orange-yellow or orange red autumn, behind the winter leaf abscission, its cinnamon to the trunk of the shape that scales off of white has very high ornamental value equally.It is strong that white birch with purple leaf also has resistance to insects, happiness illumination, humidity, wide adaptability, the easily characteristics of plantation.Because often showing as and spend no fertility, do not produce the seed of seed or generation, white birch with purple leaf can not form plantlet, so breed in the mode of epicormic branch cuttage or grafting always with genetic stability proterties.White birch with purple leaf is introduced China via the form of aseptic seedling in vitro, after the introduction through transplant survival, but the limited amount of introducing, and cost height, difficulty is accomplished scale production; If utilizing the mode of epicormic branch cuttage or grafting breeds; exist the nursery stock brephic long; breeding cycle is long; the problem that proliferate efficiency is low, plant percent is not high; therefore be difficult to realize the numerous and large-scale production of a large amount of expansions of white birch with purple leaf nursery stock at short notice, the popularization cultivation and the utilization of resources of white birch with purple leaf are greatly limited.
Summary of the invention
The objective of the invention is long for the breeding cycle that solves white birch with purple leaf, proliferate efficiency is low, cost is high, survival rate is low and the problem of genetic stability difference, and the micro-propagation method of a kind of white birch with purple leaf that provides.
The micro-propagation method of white birch with purple leaf is realized according to the following steps: the white birch with purple leaf explant after, will cleaning is 75% alcohol solution dipping 60s again with another part mass concentration after on the superclean bench being 75% alcohol solution dipping 60s with mass concentration, use aseptic water washing then 3～5 times, be 0.1% mercuric chloride solution sterilization, 12～15min with mass concentration again, use aseptic water washing then 3～5 times; Two, the explant after will sterilizing cuts into that to have terminal bud or 2～3 axillalry buds and length be the stem section of 1.5～3cm, be inoculated in then in the inducing culture, and be that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～30 days change under the condition of fresh inducing culture and cultivated 4～6 months, obtains little (unrooted test-tube plantlet); Three, little blade being removed, cut into that to have terminal bud or 1～2 axillalry bud and length be the stem section of 1～1.5cm, be inoculated in then in the proliferated culture medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～25 days change that to be cultured to little branch growth length under the condition of fresh proliferated culture medium be 0.5～1.5cm; Four, the little branch after will breeding is inoculated in and increases in the strong medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h condition under to be cultured to little branch growth length be 2.5～4.5cm, cuttage is carried out promptly finishing the little numerous of white birch with purple leaf after the culture of rootage in the matrix of taking root again; Wherein inducing culture is to be minimal medium with the WPM medium in the step 2, and also comprises the 6-benzylaminopurine (6-BA) of 0.2～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Proliferated culture medium is to be minimal medium with the WPM medium in the step 3, and also comprises the 6-benzylaminopurine (6-BA) of 0.5～1.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Increasing strong medium in the step 4 and be with the WPM medium is minimal medium, and also comprises the 6-benzylaminopurine (6-BA) of 0.1～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; The matrix of taking root in the step 4 by volume portion rate is made up of 5 parts the peat composed of rotten mosses, 4 parts vermiculite and 4 parts perlite, and the water content of substrate of taking root is 50%～60%.
White birch with purple leaf is little numerous among the present invention, and the breeding cycle is short, and efficient height, its proliferate efficiency are 30～40 times of per 30～40 days propagation, and utilizes original terminal bud on the maternal plant or axillalry bud directly to carry out the propagation of bud, the descendant inheritting good stability that is produced; Increase little branch of strengthening after cultivating and outside test tube, can directly take root into complete regeneration plant with plant hormone without any need for chemical reagent, take root and tame simultaneously and carry out, operating technology is simple, saving of work and time, cost is low, save 30%～50% expense than existing method, and rooting rate and the survival rate of transplanting seedlings all can reach 100%, realized white birch with purple leaf fast, the efficient target of breeding and large-scale promotion plantation.
Embodiment
Embodiment one: the micro-propagation method of present embodiment white birch with purple leaf is realized according to the following steps: the white birch with purple leaf explant after, will cleaning is 75% alcohol solution dipping 60s again with another part mass concentration after on the superclean bench being 75% alcohol solution dipping 60s with mass concentration, use aseptic water washing then 3～5 times, be 0.1% mercuric chloride solution sterilization, 12～15min with mass concentration again, use aseptic water washing then 3～5 times; Two, the explant after will sterilizing cuts into that to have terminal bud or 2～3 axillalry buds and length be the stem section of 1.5～3cm, be inoculated in then in the inducing culture, and be that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～30 days change under the condition of fresh inducing culture and cultivated 4～6 months, obtains little (unrooted test-tube plantlet); Three, little blade being removed, cut into that to have terminal bud or 1～2 axillalry bud and length be the stem section of 1～1.5cm, be inoculated in then in the proliferated culture medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～25 days change that to be cultured to little branch growth length under the condition of fresh proliferated culture medium be 0.5～1.5cm; Four, the little branch after will breeding is inoculated in and increases in the strong medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h condition under to be cultured to little branch growth length be 2.5～4.5cm, cuttage is carried out promptly finishing the little numerous of white birch with purple leaf after the culture of rootage in the matrix of taking root again; Wherein inducing culture is to be minimal medium with the WPM medium in the step 2, and also comprises the 6-benzylaminopurine (6-BA) of 0.2～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Proliferated culture medium is to be minimal medium with the WPM medium in the step 3, and also comprises the 6-benzylaminopurine (6-BA) of 0.5～1.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Increasing strong medium in the step 4 and be with the WPM medium is minimal medium, and also comprises the 6-benzylaminopurine (6-BA) of 0.1～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; The matrix of taking root in the step 4 by volume portion rate is made up of 5 parts the peat composed of rotten mosses, 4 parts vermiculite and 4 parts perlite, and the water content of substrate of taking root is 50%～60%.
Selecting the tender plant of eugonic children for gathering the maternal plant of explant in the present embodiment, is explant with the branch of new sprouting in spring or the branch of manually facilitating.
Cleaning in the present embodiment step 1 is to clean explant (can add a washing agent in the water) with clear water, and the dust and the assorted bacterium of scrubbing the branch surface gently with the fine, soft fur brush during cleaning are noted not damaging sleeping bud, then branch are washed 0.5～1h under running water.
The stem section is inoculated in proliferated culture medium in the present embodiment step 3, is that the stem section with the tool terminal bud vertically is inserted in the proliferated culture medium, and the degree of depth is 0.5cm; The stem section of tool axillalry bud is lain in the enrichment culture primary surface.
Little enrichment culture in the present embodiment step 3, proliferate efficiency are 30～40 times of per 30～40 days propagation, can be transferred in the fresh proliferated culture medium and to continue to cultivate, and step 3 is capable of circulation carries out, until producing little the quantity that can satisfy the demands.
Increase strong the cultivation in the present embodiment step 4 and make thin and delicate little diameter overstriking, blade increases and thickening, can improve little the survival rate of taking root.
The culture of rootage room temperature is 22～26 ℃ in the present embodiment step 4, and humidity is 70%～80%, illumination every day 12～24h, and intensity of illumination is 100～200 μ molcm
-2S
-1
Culture of rootage can be cultivated with domestication and be carried out simultaneously in the present embodiment step 4, be with around little branch take root the matrix compacting and on take root stromal surface and little branches and leaves face water spray slightly, be covered with preservative film then and cultivating indoor cultivation; Water 2 time in the mode of spraying every day, sooner or later take off film 2～5min ventilation every day, every about 7 days, spray 1 time 0.2% the pre-preventing disease and pest of carbendazim solution, rooting rate can reach 100% when cultivating for 2 weeks, progressively remove preservative film after 2 weeks, strengthen and ventilate and illumination, the little branch growth length after taking root when cultivating 60 days can reach more than the 20cm.
In the present embodiment after little numerous the finishing of white birch with purple leaf, can carry out nursery stock transplanting, little branch growth length after promptly taking root is transplanted to (culture matrix in the little alms bowl is the mixture of turfy soil and sandy soil) in the little alms bowl of plastics after reaching 20cm, be put into then in greenhouse or the booth and cultivate, cultivation temperature is 15～30 ℃, humidity is more than 60%, notes ventilating, and transplanting survival rate reaches 100%.
Embodiment two: not being both in the step 1 of present embodiment and embodiment one cleaned flushing 4 times with sterile water, is 0.1% mercuric chloride solution sterilization 14min again with mass concentration, uses aseptic water washing then 4 times.Other step and parameter are identical with embodiment one.
Mercuric chloride solution can add a Tween-20 in the present embodiment.
Embodiment three: the explant that is not both in the step 2 of present embodiment and embodiment two cuts into that to have terminal bud or 2 axillalry buds and length be the stem section of 2cm.Other step and parameter are identical with embodiment two.
Embodiment four: present embodiment and embodiment three not to be both in the step 2 in temperature be that 24 ℃, humidity are 75%, intensity of illumination is 160 μ molcm
-2S
-1, illumination every day 16h, more renewed in per 25 days under the condition of preparation inducing culture and cultivated 5 months.Other step and parameter are identical with embodiment three.
Embodiment five: not being both of present embodiment and embodiment four cuts in the step 3 that to have terminal bud or 2 axillalry buds and length be the stem section of 1.2cm.Other step and parameter are identical with embodiment four.
Embodiment six: present embodiment and embodiment five not to be both in the step 3 in temperature be that 25 ℃, humidity are 78%, intensity of illumination is 140 μ molcm
-2S
-1, illumination every day 18h, more renewed that to be cultured to little branch growth length be 1cm under the condition of preparation proliferated culture medium in per 22 days.Other step and parameter are identical with embodiment five.
Embodiment seven: present embodiment and embodiment six not to be both in the step 4 in temperature be that 23 ℃, humidity are 76%, intensity of illumination is 180 μ molcm
-2S
-1, illumination every day 20h condition under to be cultured to little branch growth length be 3cm.Other step and parameter are identical with embodiment six.
Claims (7)
1, a kind of micro-propagation method of white birch with purple leaf, the micro-propagation method that it is characterized in that white birch with purple leaf is realized according to the following steps: the white birch with purple leaf explant after, will cleaning is 75% alcohol solution dipping 60s again with another part mass concentration after on the superclean bench being 75% alcohol solution dipping 60s with mass concentration, use aseptic water washing then 3～5 times, be 0.1% mercuric chloride solution sterilization, 12～15min with mass concentration again, use aseptic water washing then 3～5 times; Two, the explant after will sterilizing cuts into that to have terminal bud or 2～3 axillalry buds and length be the stem section of 1.5～3cm, be inoculated in then in the inducing culture, and be that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～30 days change under the condition of fresh inducing culture and cultivated 4～6 months, obtains little; Three, little blade being removed, cut into that to have terminal bud or 1～2 axillalry bud and length be the stem section of 1～1.5cm, be inoculated in then in the proliferated culture medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h, per 20～25 days change that to be cultured to little branch growth length under the condition of fresh proliferated culture medium be 0.5～1.5cm; Four, the little branch after will breeding is inoculated in and increases in the strong medium, is that 22～26 ℃, humidity are 70%～80%, intensity of illumination is 100～200 μ molcm in temperature
-2S
-1, illumination every day 12～24h condition under to be cultured to little branch growth length be 2.5～4.5cm, cuttage is carried out promptly finishing the little numerous of white birch with purple leaf after the culture of rootage in the matrix of taking root again; Wherein inducing culture is to be minimal medium with the WPM medium in the step 2, and also comprises the 6-benzylaminopurine of 0.2～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Proliferated culture medium is to be minimal medium with the WPM medium in the step 3, and also comprises the 6-benzylaminopurine of 0.5～1.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; Increasing strong medium in the step 4 and be with the WPM medium is minimal medium, and also comprises the 6-benzylaminopurine of 0.1～0.5mg/L, the sucrose of 20～30g/L and the agar of 6～7g/L, and the pH value is 5.2; The matrix of taking root in the step 4 by volume portion rate is made up of 5 parts the peat composed of rotten mosses, 4 parts vermiculite and 4 parts perlite, and the water content of substrate of taking root is 50%～60%.
2, the micro-propagation method of a kind of white birch with purple leaf according to claim 1 is characterized in that using in the step 1 aseptic water washing 4 times, is 0.1% mercuric chloride solution sterilization 14min again with mass concentration, uses aseptic water washing then 4 times.
3, the micro-propagation method of a kind of white birch with purple leaf according to claim 2 is characterized in that in the step 2 that explant cuts into that to have terminal bud or 2 axillalry buds and length be the stem section of 2cm.
4, the micro-propagation method of a kind of white birch with purple leaf according to claim 3 is characterized in that in the step 2 in temperature being that 24 ℃, humidity are 75%, intensity of illumination is 160 μ molcm
-2S
-1, illumination every day 16h, per 25 days change under the condition of fresh inducing culture and cultivated 5 months.
5, the micro-propagation method of a kind of white birch with purple leaf according to claim 4 is characterized in that cutting in the step 3 that to have terminal bud or 2 axillalry buds and length be the stem section of 1.2cm.
6, the micro-propagation method of a kind of white birch with purple leaf according to claim 5 is characterized in that in the step 3 in temperature being that 25 ℃, humidity are 78%, intensity of illumination is 140 μ molcm
-2S
-1, illumination every day 18h, per 22 days change that to be cultured to little branch growth length under the condition of fresh proliferated culture medium be 1cm.
7, the micro-propagation method of a kind of white birch with purple leaf according to claim 6 is characterized in that in the step 4 in temperature being that 23 ℃, humidity are 76%, intensity of illumination is 180 μ molcm
-2S
-1, illumination every day 20h condition under to be cultured to little branch growth length be 3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100713455A CN101502237B (en) | 2009-01-24 | 2009-01-24 | Micro-propagation method for white birch with purple leaf |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100713455A CN101502237B (en) | 2009-01-24 | 2009-01-24 | Micro-propagation method for white birch with purple leaf |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101502237A true CN101502237A (en) | 2009-08-12 |
| CN101502237B CN101502237B (en) | 2011-06-15 |
Family
ID=40974731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100713455A Expired - Fee Related CN101502237B (en) | 2009-01-24 | 2009-01-24 | Micro-propagation method for white birch with purple leaf |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101502237B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972301A (en) * | 2012-12-08 | 2013-03-20 | 四川农业大学 | Tissue-culture seedling raising method for red birch |
| CN103026886A (en) * | 2012-12-28 | 2013-04-10 | 重庆新开源农业开发有限公司 | Method for growing water birch seedlings in Three Gorges reservoir area |
| CN104365485A (en) * | 2014-12-05 | 2015-02-25 | 浙江农林大学 | Betula luminifera tissue culture seedling rooting substrate and rooting and transplanting method thereof |
| CN105177039A (en) * | 2015-10-26 | 2015-12-23 | 东北林业大学 | Method for transferring exogenous genes into betula platyphylla Suks. seedlings for transient expression |
| CN106171996A (en) * | 2016-07-21 | 2016-12-07 | 辽宁大学 | A kind of method for quickly breeding of wild Betula platyphylla Suk. |
| CN114586686A (en) * | 2022-04-06 | 2022-06-07 | 东北林业大学 | Method for efficiently transplanting tissue culture seedlings of betula schizophylla |
| CN115136887A (en) * | 2022-08-04 | 2022-10-04 | 东北林业大学 | Method for cultivating triploid purple rained birch |
-
2009
- 2009-01-24 CN CN2009100713455A patent/CN101502237B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972301A (en) * | 2012-12-08 | 2013-03-20 | 四川农业大学 | Tissue-culture seedling raising method for red birch |
| CN103026886A (en) * | 2012-12-28 | 2013-04-10 | 重庆新开源农业开发有限公司 | Method for growing water birch seedlings in Three Gorges reservoir area |
| CN104365485A (en) * | 2014-12-05 | 2015-02-25 | 浙江农林大学 | Betula luminifera tissue culture seedling rooting substrate and rooting and transplanting method thereof |
| CN104365485B (en) * | 2014-12-05 | 2016-09-21 | 浙江农林大学 | A kind of smoothbark birch tissue cultured seedling is taken root the method for substrate and rooting and transplant thereof |
| CN105177039A (en) * | 2015-10-26 | 2015-12-23 | 东北林业大学 | Method for transferring exogenous genes into betula platyphylla Suks. seedlings for transient expression |
| CN105177039B (en) * | 2015-10-26 | 2020-04-21 | 东北林业大学 | Transient expression method for exogenous gene transferred birch seedling |
| CN106171996A (en) * | 2016-07-21 | 2016-12-07 | 辽宁大学 | A kind of method for quickly breeding of wild Betula platyphylla Suk. |
| CN106171996B (en) * | 2016-07-21 | 2018-02-09 | 辽宁大学 | A kind of rapid propagation method of wild white birch |
| CN114586686A (en) * | 2022-04-06 | 2022-06-07 | 东北林业大学 | Method for efficiently transplanting tissue culture seedlings of betula schizophylla |
| CN115136887A (en) * | 2022-08-04 | 2022-10-04 | 东北林业大学 | Method for cultivating triploid purple rained birch |
| CN115136887B (en) * | 2022-08-04 | 2023-01-10 | 东北林业大学 | Method for cultivating triploid purple rain birch |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101502237B (en) | 2011-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101502237B (en) | Micro-propagation method for white birch with purple leaf | |
| CN101946703B (en) | Method for regenerating plants of Chinese rose by using leaves as explants | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN102657094B (en) | Ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus | |
| CN105494100A (en) | Tissue culture and rapid propagation method of oriental cherries | |
| CN111616052A (en) | Rapid propagation and sugar-free rooting culture method and application of apple rootstock catalpa bungei | |
| CN105075863A (en) | Rapid paeonia rockii reproduction method | |
| CN100429306C (en) | Tissue culture medium and fast propagation method for Sorbone lily | |
| CN103141387A (en) | Method for cultivating haworthia maughanii tissue | |
| CN103380729A (en) | Tissue culture and rapid propagation method of didymostigma obtusum | |
| CN101103702A (en) | Excised reproduction method for mountain ash | |
| CN102246700B (en) | Tissue culture method for populus yunnanensis Dode with tender stem as explant | |
| CN102415339A (en) | Rapid propagation method of photinia fraseri | |
| CN101946704B (en) | Method for regenerating Chinese rose plant by using immature seed as explant | |
| KR101531308B1 (en) | Method of propagating Paulownia coreana using tissue culture | |
| CN106165648B (en) | A kind of cercis tissue culture culture medium and cultural method | |
| CN102613089B (en) | High-efficient in-vitro propagation method of 20-year-old schima superba big tree | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN107568069B (en) | A kind of smoothbark birch tissue-cultured seedling high efficiently multiplying method | |
| CN108271693A (en) | One kind posting tree orchid regenerating system quickly foundation and store method | |
| CN101502238B (en) | Micro-propagation method for Populus hybride | |
| Zaki et al. | A reproducible protocol for raising clonal plants from leaf segments excised from mature trees of Betula utilis a threatened tree species of Kashmir Himalayas | |
| CN104054579B (en) | A kind of method of tung oil tree petiole directly regenerated plant | |
| CN104488726A (en) | Outside-bottle rooting method of radix pseudostellariae test-tube plantlets | |
| CN109548653A (en) | A kind of quick mating system of tissue cultures for the winter red fruit that makes a variation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110615 Termination date: 20120124 |