CN106140804A - One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil - Google Patents
One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil Download PDFInfo
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- CN106140804A CN106140804A CN201610562782.7A CN201610562782A CN106140804A CN 106140804 A CN106140804 A CN 106140804A CN 201610562782 A CN201610562782 A CN 201610562782A CN 106140804 A CN106140804 A CN 106140804A
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- organic matter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
- B09C1/085—Reclamation of contaminated soil chemically electrochemically, e.g. by electrokinetics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Environmental & Geological Engineering (AREA)
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- Soil Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The present invention relates to Electrokinetic Remediation of Polluted Soils technical field, specifically one utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil.Dissolved Organic Matter is put on organic polluted soil to be repaired, then by the repair mode of regulation and control electronic microorganism, soil is repaired, and during electronic microorganism remediation, add Dissolved Organic Matter, and then realize improving the remediation efficiency of persistent organic pollutants in soil.Restorative procedure of the present invention uses Dissolved Organic Matter to not only facilitate organic pollution and desorbs from soil particle, and can provide favourable carbon source for microorganism during electronic microorganism remediation.Dissolved Organic Matter used in restorative procedure of the present invention is nontoxic, can be biodegradable, environmentally safe, and low cost.
Description
Technical field
The present invention relates to Electrokinetic Remediation of Polluted Soils technical field, specifically one utilizes Dissolved Organic Matter to improve has
Machine contaminated soil is electronic-repairing method of microorganism.
Background technology
Dissolved Organic Matter is a kind of important, active chemical constituent in soil, and the soda acid that it not only affects soil is special
Property, also affect the validity of nutriment, the adsorption-desorption characteristics of polluter.Dissolved Organic Matter is to organic contamination in soil
The impact of thing adsorption-desorption has double action.On the one hand, Dissolved Organic Matter has surface-active property, and it is hydrophobic that it contains
Property component can be combined with organic pollution, increase that it is water-soluble, make adsorption capacity in soil for the organic pollution decline or promote
Entering organic pollution desorption on soil for the absorption makes it discharge in solution.On the other hand, Dissolved Organic Matter is understood and some
The adsorption site of organic pollution competition soil surface, produces competitive Adsorption, thus reduces the adsorption capacity of organic pollution, have
It is beneficial to the desorption of organic contaminants in soil, improve its concentration in the soil liquid.Additionally, Dissolved Organic Matter can be micro-life
Thing provides Co metabolism substrate to promote the removal of persistent organic pollutants.
Electronic-microorganism remediation is increasingly subject to people's attention as a kind of new organic polluted soil recovery technique,
But due to organic pollution especially persistent organic pollutants bioavailability difference, and hydrophobicity is strong, water-soluble low, pole
Easily be combined with soil particle, thus reduce soil electronic-microorganism remediation efficiency.In electrokinetic process, in soil, apply table
Face activating agent can be effectively promoted the desorption of pollutant in soil, improves the bioavailability of organic pollution, strengthens electricity
Dynamic remediation efficiency.Studies have found that owing to partially expensive and in soil the utilization rate of the price of surfactant own is relatively low, and
Some surfactants are possible to cause secondary pollution to soil due to itself toxicity or refractory organics.Therefore adopting
Its environment friendly and economic serviceability should be taken into full account when strengthening electrokinetic remediation efficiency with cosolvent.
The complex mixture that Dissolved Organic Matter is made up of multiple organic compounds, including organic acid, polysaccharide, polypeptide with
And amino acid etc..Comparing with other surfactants, Dissolved Organic Matter is nontoxic, can be biodegradable and to environment without dirt
Dye.Therefore, utilize Dissolved Organic Matter to improve electronic-microorganism remediation to have broad application prospects.
Content of the invention
Present invention aim at provide one utilize Dissolved Organic Matter improve organic polluted soil electronic-microorganism remediation
Method.
For achieving the above object, the technical solution used in the present invention is:
One utilize Dissolved Organic Matter improve organic polluted soil electronic-repairing method of microorganism, dissolubility is organic
Matter puts on organic polluted soil to be repaired, is then repaired soil by the repair mode of electronic-microorganism, and at electricity
Add Dissolved Organic Matter during dynamic-microorganism remediation, and then realize the reparation to persistent organic pollutants in soil
Efficiency improves.
The addition of described Dissolved Organic Matter is according to desorption in soil for the pollutant in organic polluted soil to be repaired
Depending on attached indicatrix, using desorption of contaminants amount reach maximum when Dissolved Organic Matter content add in soil as benchmark
Dissolved Organic Matter；Applying Dissolved Organic Matter in soil, in soil, Dissolved Organic Matter concentration is not less than 20mgC/kg, protects
The maximum that card desorption of contaminants needs.
Described Dissolved Organic Matter is to obtain after processing organic fertilizer pig manure or fertilizer green manure.
Wherein, organic fertilizer pig manure is the pig manure after fully becoming thoroughly decomposed.
Fertilizer green manure is the fertilizer that fresh straw is made after stack retting.
Described Dissolved Organic Matter is to mix organic fertilizer pig manure or fertilizer green manure with water according to the solid-to-liquid ratio of 1:10-40
Close (material dry weight g；Ultra-pure water volume ml), after mixing 180-200r/min at room temperature horizontal constant temperature oscillation machine on vibration
16-20h, then in 4 DEG C, centrifugal 20-30min goes the removal of impurity under 12000-15000r/min, supernatant is through the filter membrane of 0.45 μm
Filter, collect filtrate and be Dissolved Organic Matter.
Described electronic-microorganism remediation condition: electric field uses switching electric field, and each switching time is 5-10min, and voltage is
0.5-2v/cm, content of microorganisms is 107-108Individual/gram dry ground, soil moisture is 20-25%.
Wherein, microorganism can be Bacillus cereus, bacillus licheniformis or Pseudomonas fluorescens.
Advantage for present invention:
1. restorative procedure of the present invention uses Dissolved Organic Matter to not only facilitate organic pollution and desorb from soil particle,
And favourable carbon source can be provided for microorganism during electronic-microorganism remediation.
2. Dissolved Organic Matter used in restorative procedure of the present invention is nontoxic, can be biodegradable, environmentally safe,
And low cost.
Brief description
Electronic-microorganism remediation device schematic diagram that Fig. 1 provides for the embodiment of the present invention.
It is bent with Dissolved Organic Matter change in concentration that Fig. 2 is that the embodiment of the present invention 1 processes in front contaminated soil PAHs desorption efficiency
Line.
Fig. 3 is PAHs clearance change curve in the embodiment of the present invention 1 processing procedure.
Fig. 4 is that the embodiment of the present invention 1 applies PAHs clearance change curve after Dissolved Organic Matter when processing 70 days.
Detailed description of the invention
Further illustrate technical scheme below by way of specific embodiment.
Electronic-microorganism remediation device such as Fig. 1 used by embodiment.In figure, stainless steel electrode 1, soil chamber and Polluted Soil
Earth 2, power supply/automatic monitored control system 3, sampled point 4.
Insert stainless steel electrode 1 at soil chamber or contaminated soil to be repaired 2 two ends of accommodating contaminated soil to be repaired respectively,
The stainless steel electrode that two ends are inserted is connected with power supply/automatic monitored control system 3 by wire, and is provided with on contaminated soil to be repaired
Multiple sampled points 4.
The inventive method utilizes the surface-active property of Dissolved Organic Matter and can provide Co metabolism carbon source for microorganism
Characteristic strengthen difficult degradation organic polluted soil electronic-microorganism remediation.
The method Dissolved Organic Matter content required when reaching maximum with repair process pollutant in soil desorption quantity
Based on, in soil, applying Dissolved Organic Matter, in soil, Dissolved Organic Matter concentration is not less than 20mgC/kg, it is ensured that pollute
The maximum that thing desorption needs, also can meet the carbon source needed for microorganism Co metabolism simultaneously, improves electronic-micro-life of contaminated soil
The overall remediation efficiency of thing.
Embodiment 1
Soil used by the present embodiment is coking place PAHs contaminated soil.The soil adopted back is gone the removal of impurity, air-dries, mill
Carefully cross 20 mesh sieves standby.In soil, Dissolved Organic Matter content is 35mg C/kg, and polycyclic aromatic hydrocarbon content is 280.3mg/kg, three
Ring above PAHs content is 85.2%.Contaminated soil is successively loaded soil chamber, is compacted standby.
The present embodiment arranges 2 process, wherein: process 1 as comparison, before treatment with processing procedure in without dissolving
Property organic；Process 2 before experiment, add Dissolved Organic Matter, and carry out molten according to the change of PAHs clearance in processing procedure
Solution property is organic adds, and depending on addition is according to desorption features curve in soil for the pollutant, reaches with desorption of contaminants amount
Add Dissolved Organic Matter to Dissolved Organic Matter content during maximum as benchmark in soil.
Dissolved Organic Matter extracts from organic green manure pig manure, by organic green manure pig manure and water according to the solid-to-liquid ratio (thing of 1:20
Material dry weight g；Ultra-pure water volume ml) mixing, at 25 DEG C 200r/min horizontal constant temperature oscillation machine on vibration 16h, then in 4
DEG C, centrifugal 20min concentrates under 12000r/min, the filter membrane of 0.45 μm crossed by supernatant, and the organic matter in filtrate is dissolving
Property organic, use TOC instrument to measure its concentration 2630mg C/L.
(polycyclic aromatic hydrocarbon-degrading bacteria is Bacillus cereus, bacillus licheniformis to 2 process all inoculation polycyclic aromatic hydrocarbon-degrading bacterias
Or Pseudomonas fluorescens) and apply DC electric field, inoculum concentration is 3.6 × 107Cfu/g dry ground, electric-field intensity is 1.0V/cm, adopts
By the method for operation of polarity of electrode switching, polarity switching time is spaced apart 5min, and soil moisture is 25%.Periodic sampling, follows the tracks of
PAHs clearance change in monitoring soil, when PAHs clearance reaches maximum and changes little, in soil, complement lysis has
Machine matter, depending on the maximal solution pipette according to PAHs in soil at that time for the Dissolved Organic Matter magnitude of recruitment.
By measurement result it can be seen that when Dissolved Organic Matter concentration is 210mg C/kg, process in front contaminated soil
The desorption efficiency of PAHs can reach maximum, continues to add Dissolved Organic Matter, and the change of PAHs desorption efficiency is little (Fig. 2).Therefore, it is
Ensure the maximum desorption efficiency of contaminated soil PAHs, process interpolation SOM in 2 contaminated soils at experiment forward direction, make soil
In earth, the concentration of SOM is 210mg C/kg.Fig. 3 is shown PAHs clearance change in 2 process, processes in 2
Add the PAHs clearance of the clearance of PAHs after dissolved organic carbon apparently higher than the process 1 being not added with dissolved organic carbon.
The clearance processing 1 when 60 days reaches maximum, and processes 2 and reached maximum when 70 days, and PAHs clearance is than process 1
High by 11%.When 70 days according to the amount that the maximum solubility needing during PAHs desorption in soil is organic, continue to add in soil
Add Dissolved Organic Matter, make Dissolved Organic Matter content in soil be 175mg C/kg, PAHs concentration in results of regular determination soil.Knot
Really display, after adding Dissolved Organic Matter, the clearance of PAHs continues to increase, and PAHs clearance improves 15.4%, with process 1
Comparing, PAHs clearance improves 26.7%.
Claims (5)
1. one kind utilize Dissolved Organic Matter improve organic polluted soil electronic-repairing method of microorganism, it is characterised in that: by molten
Solution property organic matter puts on organic polluted soil to be repaired, is then repaiied soil by the repair mode of electronic-microorganism
Multiple, and during electronic-microorganism remediation, add Dissolved Organic Matter, and then realize to difficult degradation organic contamination in soil
The remediation efficiency of thing improves.
2. as described in claim 1 utilize Dissolved Organic Matter improve organic polluted soil electronic-repairing method of microorganism, its
It is characterised by: the addition of described Dissolved Organic Matter is according to desorption in soil for the pollutant in organic polluted soil to be repaired
Attached indicatrix, using desorption of contaminants amount reach maximum when Dissolved Organic Matter content in soil, add dissolving as benchmark
Property organic.
3. as described in claim 1 or 2 utilize Dissolved Organic Matter improve organic polluted soil electronic-microorganism remediation side
Method, it is characterised in that: described Dissolved Organic Matter is to obtain after processing organic fertilizer pig manure or fertilizer green manure.
4. as described in claim 3 utilize Dissolved Organic Matter improve organic polluted soil electronic-repairing method of microorganism, its
It is characterised by: described Dissolved Organic Matter is according to the solid-to-liquid ratio of 1:10-40 and water by organic fertilizer pig manure or fertilizer green manure
Mixing, after mixing 180-200r/min at room temperature horizontal constant temperature oscillation machine on vibration 16-20h, then in 4 DEG C, 12000-
Under 15000r/min, centrifugal 20-30min goes the removal of impurity, and supernatant, through membrane filtration, is collected filtrate and is Dissolved Organic Matter.
5. utilize as described in claim 1 Dissolved Organic Matter improve organic polluted soil electronic-repairing method of microorganism, it is special
Levy and be: described electronic-microorganism remediation condition: electric field uses switching electric field, and each switching time is 5-10min, and voltage is
0.5-2v/cm, content of microorganisms is 107-108Individual/gram dry ground, soil moisture is 20-25%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610562782.7A CN106140804A (en) | 2016-07-18 | 2016-07-18 | One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil |
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| CN201610562782.7A CN106140804A (en) | 2016-07-18 | 2016-07-18 | One utilizes Dissolved Organic Matter to improve the electronic repairing method of microorganism of organic polluted soil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646587A (en) * | 2020-11-25 | 2021-04-13 | 宁波城市阳光环境建设工程有限公司 | Soil remediation composition, preparation method thereof and soil bioremediation method |
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| CN102225426A (en) * | 2011-03-14 | 2011-10-26 | 哈尔滨工业大学 | Method for electrically remedying soil polluted by petroleum |
| CN103302087A (en) * | 2013-06-08 | 2013-09-18 | 四川农业大学 | Method for repairing lead and zinc polluted soil by utilizing accumulator plants-ryegrass and pig manure |
| CN104588405A (en) * | 2015-01-19 | 2015-05-06 | 中国科学院沈阳应用生态研究所 | Microorganism-electric power two-segment repairing method for petroleum contaminated soils |
| CN104801537A (en) * | 2015-04-09 | 2015-07-29 | 上海大学 | Electric-microbial combined remediation method for heavy metal contaminated soil |
| CN105689387A (en) * | 2016-03-30 | 2016-06-22 | 中国石油大学(华东) | Method for restoring combined pollution soil through electrodynamic force-fuel cell coupling device |
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2016
- 2016-07-18 CN CN201610562782.7A patent/CN106140804A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225426A (en) * | 2011-03-14 | 2011-10-26 | 哈尔滨工业大学 | Method for electrically remedying soil polluted by petroleum |
| CN103302087A (en) * | 2013-06-08 | 2013-09-18 | 四川农业大学 | Method for repairing lead and zinc polluted soil by utilizing accumulator plants-ryegrass and pig manure |
| CN104588405A (en) * | 2015-01-19 | 2015-05-06 | 中国科学院沈阳应用生态研究所 | Microorganism-electric power two-segment repairing method for petroleum contaminated soils |
| CN104801537A (en) * | 2015-04-09 | 2015-07-29 | 上海大学 | Electric-microbial combined remediation method for heavy metal contaminated soil |
| CN105689387A (en) * | 2016-03-30 | 2016-06-22 | 中国石油大学(华东) | Method for restoring combined pollution soil through electrodynamic force-fuel cell coupling device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646587A (en) * | 2020-11-25 | 2021-04-13 | 宁波城市阳光环境建设工程有限公司 | Soil remediation composition, preparation method thereof and soil bioremediation method |
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Application publication date: 20161123 |
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