CN104588405A - Microorganism-electric power two-segment repairing method for petroleum contaminated soils - Google Patents
Microorganism-electric power two-segment repairing method for petroleum contaminated soils Download PDFInfo
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Abstract
The invention belongs to the technical field of environmental treatment and in particular relates to a microorganism-electric power two-segment repairing method for petroleum contaminated soils. According to the microorganism-electric power two-segment repairing method disclosed by the invention, conversion between two segments including microorganism repairing and electrokinetic repairing is carried out with the ratio of the total petroleum hydrocarbon content and the total organic carbon content in contaminated soils, and the purpose of preparing the petroleum contaminated soils is achieved; microorganisms are capable of preferentially degrading light components which are easily degraded in petroleum hydrocarbon and then removing heavy components by virtue of the strengthening effect of an electric field, and staged degradation of pollutants is realized; the problem that the pollutants can not be completely degraded by virtue of single microorganisms is solved, and the energy consumption by virtue of single electrokinetic remediation is also lowered; the microorganism-electric power two-segment repairing method is rapid in speed for repairing the petroleum contaminated soils, high in efficiency and low in cost; and compared with single microorganism repairing and single electrokinetic repairing, the efficiency is improved by above 20-40 percent.
Description
Technical field
The invention belongs to technical field of environmental management, be specifically related to a kind of microorganism-electronic two-part restorative procedure of oil-polluted soils.
Background technology
Soil petroleum pollution has become the environmental problem that can not be ignored, and oil-polluted soils reparation receives the attention of height at home and abroad.Current existing recovery technique comprises peripheral doses technology, chemical remediation technology and bioremediation technology.Bioremediation technology typically refers to microorganism remediation, is a kind of recovery technique grown up on microbial degradation basis, compared with physics, chemical remediation technology, has that cost is low, not spoiled soil environment, non-secondary pollution and the feature such as simple to operate.But microorganism remediation is larger by soil pollutant properties influence, microorganism can alkane in preferential degradation petroleum hydrocarbon and aromatic hydrocarbons, and even can not degrade to the degradation efficiency of gum asphalt is extremely low, cause that alkane and aromatic hydrocarbons in soil sharply decline like this, gum asphalt ratio increases.A large amount of accumulations of gum asphalt can suppress growth of microorganism, reduce the remediation efficiency of microorganism, and therefore, the simple biological method that relies on is difficult to thoroughly repair oil-polluted soils, needs the removal of combining other technique heavy constituent.
Electric repairing technique is considered to the effective technology solving oil-polluted soils, and its general principle is that the various electrokinesises utilizing electric field to produce strengthen the bioavailability of pollutant in underground environment, the activity of microorganism or direct mineralising organic matter.Because this technology has, rehabilitation cost is low, removal efficiency high, receives the extensive concern of domestic and international soil remediation expert.Electric repairing technique has been applied in the reparation of high-concentration hardly-degradable organic polluted soil (as PAHs, petroleum hydrocarbon etc.) in recent years, and achieves better repairing effect.But in order to reduce simple employing electric repairing technique energy consumption, therefore correlative study is more prone to combined remediation technology exploitation, carries out the pollutant combining to remove in soil by other technologies and electric repairing technique.In order to more cost-saving, improve remediation efficiency, the combination of bioremediation technology and electric repairing technique, compared to other method, more meets the needs of in-situ immobilization and Practical Project.
Soil organic matter is the general name of various carbon compound in soil, is the biological prosthetic basis of contaminated soil, and its content and composition all play decisive role to biological prosthetic feasibility and remediation efficiency.Petroleum hydrocarbon is a kind of organic compound of carbon containing, therefore the organic carbon content after oil pollution in soil obviously increases, large quantity research shows oil-polluted soils organic carbon content and oil content correlation, and organic carbon consumption decides biodegradation rate and palliating degradation degree.Therefore, the ratio based on total petroleum hydrocarbons content in contaminated soil and total content of organic carbon determines the binding site of microorganism remediation and electro reclamation two-stage process, can be effectively connected by two-stage process, shortens repair time, improves the remediation efficiency of petroleum hydrocarbon.
Summary of the invention
The object of the present invention is to provide a kind of microorganism of oil-polluted soils-electronic two-part restorative procedure.
For achieving the above object, the technical solution used in the present invention is:
Microorganism-electronic two-part restorative procedure of oil-polluted soils, with the ratio of total petroleum hydrocarbons content in contaminated soil and total content of organic carbon, carries out the conversion of microorganism remediation and electro reclamation two-part, and then reaches the object of remedying oil-polluted soils.
The transfer point of described microorganism remediation and electro reclamation two-part is the ratio of total petroleum hydrocarbons content and total content of organic carbon in contaminated soil is 0.6;
During total petroleum hydrocarbons content in soil/total content of organic carbon <0.6, adopt the mode process contaminated soil of microorganism remediation;
In soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, adopt the mode process contaminated soil of electro reclamation.
Two-part restorative procedure further,
(1) measure total petroleum hydrocarbons content and total content of organic carbon in pending contaminated soil, during total petroleum hydrocarbons content/total content of organic carbon <0.6, inoculation degradation bacteria strains carries out microorganism remediation in pending contaminated soil; Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation;
In pending contaminated soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, in soil, add organic carbon make total petroleum hydrocarbons content/total content of organic carbon <0.6, and then adopt microorganism remediation contaminated soil; Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation;
(2) during microorganism remediation when total petroleum hydrocarbons content/total content of organic carbon >=0.6, start electro reclamation.
During described microorganism remediation, degradation bacteria is inoculated in contaminated soil, temperature is 15-30 DEG C, humidity be 15-35%, pH is 6.0-8.0, repair; Wherein, the inoculum concentration of degradation bacteria is 5-8 × 10
8cfu/g dry ground;
Described degradation bacteria strains is bacillus licheniformis PS5 (Bacillus licheniformis PS5), culture presevation number is CCTCC NO:M2011261, preservation date is on July 19th, 2011, preservation mechanism is China typical culture collection center, and address is China. Wuhan. and Wuhan University.
Described organic carbon refer to humic acid, mud, fertilizer one or more.
During described electro reclamation, apply direct current to soil, the voltage gradient of soil is 1-1.5V/cm, and adopt the method for operation that polarity of electrode switches, the polarity switching interval time is 5-10min, and keeping soil moisture to be 15-35%, pH is 5.5-8.5.
Meanwhile, two-part restorative procedure is preferably applicable to soil petroleum hydrocarbon content lower than 8% contaminated soil.
Advantage of the present invention is: the ratio based on total petroleum hydrocarbons content in contaminated soil and total content of organic carbon determines the binding site of microorganism remediation and electro reclamation two-stage process, and the process conditions of biological prosthetic and electro reclamation before and after binding site, microorganism and electric repair method are effectively combined, first microorganism is preferentially by the degraded of the light component of easily degraded in petroleum hydrocarbon, and then remove heavy constituent by the invigoration effect of electric field, realize the stage degraded of pollutant.The present invention had both avoided the problem that pollutant can not thoroughly be degraded by independent microorganism, again reduced independent electro reclamation energy consumption.Employing the inventive method remedying oil-polluted soils speed is fast, efficiency is high, cost is low, and can effectively shorten the oil-polluted soils processing time, more independent microorganism remediation, electro reclamation raise the efficiency more than 20-40%.
Accompanying drawing explanation
Total petroleum hydrocarbons content in the Liaohe Oil Field soil that Fig. 1 provides for the embodiment of the present invention/total content of organic carbon ratio change and removal rate of petroleum hydrocarbons variation diagram.
Total petroleum hydrocarbons content in the Shengli Oil Field soil that Fig. 2 provides for the embodiment of the present invention/total content of organic carbon ratio change and removal rate of petroleum hydrocarbons variation diagram.
Detailed description of the invention
Technical scheme of the present invention is further illustrated below by way of specific embodiment.
Embodiment 1 Liaohe Oil Field oil-polluted soils is repaired
Test contaminated soil used and pick up from well site, Liaohe Oil Field periphery (Liaoning Panjin).The soil adopted back is removed impurity, air-dry, levigate mistake 20 mesh sieve is for subsequent use.In soil, total content of organic carbon is 5.21%, and total petroleum hydrocarbons content is 3.45%, and total petroleum hydrocarbons content/total content of organic carbon is 0.66.
Before carrying out microorganism-electronic two-part and repairing, in contaminated soil, be evenly mixed into the high fertilizer of organic carbon content, in soil, total petroleum hydrocarbons content/total content of organic carbon is 0.45 after measured.The contaminated soil prepared is loaded long 30cm, wide 24cm, in the soil chamber of high 15cm.
The present embodiment establishes 3 process, and be respectively independent microbiological treatment, separately electrokinetic process and microorganism-electronic two stage process, actual conditions is in table 1.The cultured petroleum hydrocarbon degradation bacterium of microbiological treatment inoculation, inoculum concentration is 5.2 × 10
8cfu/g dry ground.Electrokinetic process does not inoculate petroleum hydrocarbon degradation bacterium, but applies DC electric field, and electric-field intensity is 1.2V/cm, and adopt the method for operation that polarity of electrode switches, the polarity switching interval time is 8min.Microorganism-electronic two stage process first inoculates the after-applied DC electric field of petroleum hydrocarbon degradation bacterium, and petroleum hydrocarbon degradation bacterium inoculum concentration is 5.2 × 10
8cfu/g dry ground, electric-field intensity is 1.2V/cm, the method for operation that electric field adopts polarity to switch, and the polarity switching interval time is 8min; Microorganism-electronic two stage process convergence condition is when in soil during total petroleum hydrocarbons content/total content of organic carbon>=0.6, and now petroleum hydrocarbon is removed speed and slowed down, and adopts the mode applying DC electric field to continue repair process.
Independent electrokinetic process and the electronic Combined Treatment of microorganism electrode used are stainless steel electrode, and electrode diameter is 10mm, and electrode length is 120mm; All process maintenance soil moistures are 25 DEG C, humidity is 25%.In processing procedure, every 7d, sampling should be carried out, total petroleum hydrocarbons content and total content of organic carbon change in monitoring soil.
Can find out as microbiological treatment soil Petroleum Hydrocarbon content/total content of organic carbon >=0.6 (28d) by measurement result (Fig. 1), microorganism is to the removal rate reduction of petroleum hydrocarbon, show the reduced capability of microbial degradation petroleum hydrocarbon, and after applying DC electric field at this moment, the clearance of petroleum hydrocarbon increases rapidly, when 77d, removal rate of petroleum hydrocarbons reaches 83.7%, and more independent microbiological treatment or electrokinetic process improve 30.6%, 38.8%.It can be said that bright, at the inefficient timing node of microbiological treatment, apply the removal efficiency that electric field effectively can improve petroleum hydrocarbon, shorten the processing time.
Embodiment 2 Shengli Oil Field oil-polluted soils is repaired
Test contaminated soil used and pick up from Shengli Oil Field well site periphery (Shandong region).The soil adopted back is removed impurity, air-dry, levigate mistake 20 mesh sieve is for subsequent use.In soil, total content of organic carbon is 6.71%, and total petroleum hydrocarbons content is 2.82%, and total petroleum hydrocarbons content/total content of organic carbon is 0.42.
The present embodiment establishes 3 process, and be respectively independent microbiological treatment, separately electrokinetic process and microorganism-electronic two stage process, actual conditions is in table 2.The cultured petroleum hydrocarbon degradation bacterium of microbiological treatment inoculation, inoculum concentration is 6.8 × 10
8cfu/g dry ground.Electrokinetic process does not inoculate petroleum hydrocarbon degradation bacterium, but applies DC electric field, and electric-field intensity is 1.5V/cm, and adopt the method for operation that polarity of electrode switches, the polarity switching interval time is 5min.Microorganism-electronic two stage process first inoculates the after-applied DC electric field of petroleum hydrocarbon degradation bacterium, and petroleum hydrocarbon degradation bacterium inoculum concentration is 6.8 × 10
8cfu/g dry ground, electric-field intensity is 1.5V/cm, the method for operation that electric field adopts polarity to switch, and the polarity switching interval time is 5min; Microorganism-electronic two stage process convergence condition is when in soil during total petroleum hydrocarbons content/total content of organic carbon>=0.6, and now petroleum hydrocarbon is removed speed and slowed down, and adopts the mode applying DC electric field to continue repair process.
Independent electrokinetic process and the electronic Combined Treatment of microorganism electrode used are stainless steel electrode, and electrode diameter is 10mm, and electrode length is 120mm; All process maintenance soil moistures are 25 DEG C, humidity is 25%.In processing procedure, every 7d, sampling should be carried out, total petroleum hydrocarbons content and total content of organic carbon change in monitoring soil.
Can find out when in microbiological treatment soil time total petroleum hydrocarbons content/total content of organic carbon >=0.6 (21d) by measurement result (Fig. 2), microorganism is to the removal rate reduction of petroleum hydrocarbon, show the reduced capability of microbial degradation petroleum hydrocarbon, and after applying DC electric field at this moment, the clearance of petroleum hydrocarbon increases rapidly, when 70d, removal rate of petroleum hydrocarbons reaches 71.6%, and more independent microbiological treatment or electrokinetic process improve 28.4%, 40.8%.It can be said that bright, at the inefficient timing node of microbiological treatment, apply the removal efficiency that electric field effectively can improve petroleum hydrocarbon, shorten the processing time.
Table 1
Table 2
Claims (6)
1. microorganism-electronic two-part the restorative procedure of an oil-polluted soils, it is characterized in that: with the ratio of total petroleum hydrocarbons content in contaminated soil and total content of organic carbon, carry out the conversion of microorganism remediation and electro reclamation two-part, and then reach the object of remedying oil-polluted soils.
2. by the microorganism-electronic two-part restorative procedure of oil-polluted soils described in claim 1, it is characterized in that: the transfer point of described microorganism remediation and electro reclamation two-part is the ratio of total petroleum hydrocarbons content and total content of organic carbon in contaminated soil is 0.6;
During total petroleum hydrocarbons content in soil/total content of organic carbon <0.6, adopt the mode process contaminated soil of microorganism remediation;
In soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, adopt the mode process contaminated soil of electro reclamation.
3., by the microorganism-electronic two-part restorative procedure of oil-polluted soils described in claim 1, it is characterized in that:
(1) measure total petroleum hydrocarbon and total content of organic carbon in pending contaminated soil, during total petroleum hydrocarbon/total organic carbon <0.6, inoculation degradation bacteria strains carries out microorganism remediation in pending contaminated soil; Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation;
In pending contaminated soil during total petroleum hydrocarbons content/total content of organic carbon >=0.6, in soil, add organic carbon make total petroleum hydrocarbons content/total content of organic carbon <0.6, and then adopt microorganism remediation contaminated soil; Total petroleum hydrocarbons content and the change of total content of organic carbon ratio in periodic monitoring soil during reparation;
(2) during microorganism remediation when total petroleum hydrocarbons content/total content of organic carbon >=0.6, start electro reclamation.
4. by the microorganism-electronic two-part restorative procedure of oil-polluted soils described in claim 1, it is characterized in that: during described microorganism remediation, degradation bacteria is inoculated in contaminated soil, temperature is 15-30 DEG C, humidity be 15-35%, pH is 6.0-8.0, repair; Wherein, the inoculum concentration of degradation bacteria is 5-8 × 10
8cfu/g dry ground;
Described degradation bacteria strains is bacillus licheniformis (Bacillus licheniformis), and culture presevation number is CCTCC NO:M2011261, and preservation mechanism is China typical culture collection center.
5., by the microorganism-electronic two-part restorative procedure of oil-polluted soils described in claim 3, it is characterized in that: described organic carbon refer to humic acid, mud, fertilizer one or more.
6. by the microorganism-electronic two-part restorative procedure of oil-polluted soils described in claim 1, it is characterized in that: during described electro reclamation, direct current is applied to soil, the voltage gradient of soil is 1-1.5V/cm, adopt the method for operation that polarity of electrode switches, the polarity switching interval time is 5-10min, and keeping soil moisture to be 15-35%, pH is 5.5-8.5.
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