CN106116946A - A kind of bio-organic fertilizer of repairing heavy metal in soil and preparation method thereof - Google Patents

A kind of bio-organic fertilizer of repairing heavy metal in soil and preparation method thereof Download PDF

Info

Publication number
CN106116946A
CN106116946A CN201610458389.3A CN201610458389A CN106116946A CN 106116946 A CN106116946 A CN 106116946A CN 201610458389 A CN201610458389 A CN 201610458389A CN 106116946 A CN106116946 A CN 106116946A
Authority
CN
China
Prior art keywords
soil
microbial inoculum
heavy metal
bio
organic fertilizer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610458389.3A
Other languages
Chinese (zh)
Other versions
CN106116946B (en
Inventor
关统伟
李智强
王鹏昊
杨洋
邓奥宇
姚虞
赵顺先
李越
向慧平
范文斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHENGDU HUAHONG BIOTECHNOLOGY Co.,Ltd.
Original Assignee
Chengdu Huahong Biotechnology Co Ltd
Xihua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chengdu Huahong Biotechnology Co Ltd, Xihua University filed Critical Chengdu Huahong Biotechnology Co Ltd
Priority to CN201610458389.3A priority Critical patent/CN106116946B/en
Publication of CN106116946A publication Critical patent/CN106116946A/en
Application granted granted Critical
Publication of CN106116946B publication Critical patent/CN106116946B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses the bio-organic fertilizer of a kind of repairing heavy metal in soil, by animal wastes, straw, changing food waste and the microorganism mixed bacterial through special handling are made bio-organic fertilizer, it is possible to decrease soil valence heavy metal ions content more than 8%;Reduce crop heavy metal enrichment more than 10%;Reduction especially for Cr reaches more than 70%;Make crop yield more than 5%, and there is the multiple efficacies such as water conservation, fertilizer conservation and improvement Soil structure, be a kind of low cost, new bio restorative procedure efficient, easy, eco-friendly.

Description

A kind of bio-organic fertilizer of repairing heavy metal in soil and preparation method thereof
Technical field
This product relates to technical field of agricultural fertilizers, particularly relates to the biological organic fertilizer of a kind of repairing heavy metal in soil Material.
Background technology
At present, due to mankind's heavy metal exploitation, smelt, process and business manufacturing activities is increasing, cause many Heavy metal such as lead, hydrargyrum, cadmium, cobalt etc. enter in big gas and water, soil, cause serious environmental pollution, and the heavy metal pollution of soil is more It is a most serious environmental problem, has caused the highest attention of the central government, " within 2016, central authorities are to tax rebates for local governments With spending budget table " in, the budgetary resources for the obligate improvement of soil pollution are up to 90.89 hundred million yuan.Heavy metal pollution of soil thing It is mainly derived from sewage irrigation, industrial residue, municipal refuse, trade waste stacking and atmospheric sedimentation.Sewage accounts for bigger ratio The comparison of ingredients of the industrial wastewater of example is complicated, and the most in various degree be difficult to, containing biology, the various heavy degraded, be soil The main source of heavy metal contaminants.At present, the whole world discharges Hg about 1.5 ten thousand tons, Cu about 3,400,000 tons, Pb about 500 every year on average Ten thousand tons, Mn about 15,000,000 tons, Ni about 1,000,000 tons.Investigate according to the Ministry of Agriculture of China, in the Irrigation District of Sewage of about 1,400,000 hectares, suffer The land area of heavy metal pollution accounts for the 64.8% of Irrigation District of Sewage area, wherein slight pollution account for 46.7%, intermediate pollution Account for 9.7%, severe contamination account for 8.4%.
The method being used for degrading or eliminating heavy metal pollution of soil both at home and abroad substantially can be divided into Physical, chemical redemption The big class of technology, bioremediation technology etc. 3.Physical/chemical recovery technique is based primarily upon the difference of soil physico-chemical property and heavy metal Characteristic, is separated by physical chemistry means or heavy metal in fixing soil reaches cleaning soil and reduces pollutant ambient wind Danger and the technological means of health risk.Physical chemistry recovery technique includes that soil removal and replacement, soil moved in improve the original, deep ploughing such as are digged at the method, soil washing, Thermal desorption method, vitrification, electro reclamation etc..Physical chemistry technology implementation is convenient flexibly, and the cycle is shorter, it is adaptable to multiple heavy The process of metal, is used widely in the reparation of heavy-metal contaminated soil engineering, but the quantities of this technology implementation is relatively big, Relatively costly, to a certain degree its popularization and application of higher limit.Therefore, in the urgent need to a kind of low cost of development, efficient, easy, environment Friendly new bio restorative procedure.
Summary of the invention
For solving above-mentioned technical problem, the present invention provides a kind of efficient, easy, eco-friendly repairing heavy metal in soil Bio-organic fertilizer.
In the last few years, due to the raising of Agricultural Mechanization Degree, so that agricultural machinery instead of domestic animal.So that straw Amount as feedstuff greatly reduces.The when of annual crop maturity, peasant gathers in crop with a large amount of straws remaining in defensive position Just can only burning disposal.Open Burning Crop Residue atmosphere pollution, waste resource, seriously to the most affecting and just disturbing economy Often order, forms new potential safety hazard.Straw is processed and makes biological organic fertilizer, not only avoid and burn the environmental pollution brought And the wasting of resources, and the humus that Straw decomposing is become more stable by series of biochemical reactions can be passed through, supplementary nitrogen, The nutrient that the crop such as phosphorus, potassium is required, thus improve Soil structure.Changing food waste is also cause municipal pollution important simultaneously One of reason.To this end, be the most also effectively utilized the nutrition that changing food waste is abundant, fermented and be biologically converted into biology Organic fertilizer, thus turn waste into wealth, reduce municipal pollution.
According to the literature, colloid bacillus cereus (Bacillus mucilaginosus) heavy metal chromium is a kind of effective to root Microbial adsorbent (Gao Yuzhen, 2015);Be not only saccharomyces cerevisiae (Saccharomyces cerevisiae) have by bacterial strain The biological adsorption agent of practical potentiality, is also the good material (Chen Can, 2006) of research the Heavy Metals Bio-adsorption mechanism;Aspergillus niger (Aspergillus niger) can be good at preventing from heavy metal and have the ability (Fan Ting, 2012) of good enriching heavy metal.
To this end, the present invention utilizes colloid bacillus cereus, saccharomyces cerevisiae and aspergillus niger, in conjunction with animal dung, farmland straw and meal Kitchen rubbish, mixed stack preparation can the biological organic fertilizer of removing heavy-metal, will effectively reduce the toxicity of heavy metal in soil ion; , repair in conjunction with Organic Manure on Soil meanwhile, improve Soil structure, promote the absorption of Plant To Nutrient element, reach healthy and strong Plant, the inverse property in enhancing hole, raising crop yield, reduction environmental pollution and the purpose of guarantee people's life health.
A kind of bio-organic fertilizer of repairing heavy metal in soil, including the composition of following percentage by weight:
Preferably, described straw-night soil includes following main component:
Preferably, described feces of livestock and poultry is chicken manure and cattle manure mixed in equal amounts forms.
The preparation method of the bio-organic fertilizer of a kind of repairing heavy metal in soil, comprises the following steps:
(1) preparation of microbial bacterial agent
A, the preparation method of saccharomyces cerevisiae microbial inoculum: take saccharomyces cerevisiae strain, add in 30 DEG C of water constant temperature stirring until completely Dissolving, obtained liquid adds in fluid medium, detects cell density and reach 1.5-3.1 × 10 after cultivating 4-6d9cfu/ ML, obtains saccharomyces cerevisiae microbial inoculum;
B, the preparation method of colloid bacillus cereus microbial inoculum: take colloid bacillus cereus strain, be added to the water constant temperature stirring to complete CL;Obtained liquid adds in fluid medium, and at 150rpm, 30-35 DEG C, isothermal vibration cultivates 4-6d so that thalline Density reaches 6.2-8.3 × 109Cfu/mL i.e. obtains colloid bacillus cereus microbial inoculum;
C, the preparation method of aspergillus niger microbial inoculum: take aspergillus niger strain, be added to the water constant temperature and stir until being completely dissolved, institute The liquid obtained joins in inorganic salt solution, is mixed with dispensing by inorganic salt solution, is stirred inoculation, permanent at 25-30 DEG C Aspergillus niger microbial inoculum is i.e. obtained after temperature fermentation 4-6d;
(2) straw-excrement composting becomes thoroughly decomposed
Taking crushed stalk is that diameter is less than the unqualified of 2mm, adds changing food waste mixing, adds decomposing agent and animal dung Just, keeping moisture at 60%-70%, controlling compost height is 1.5 meters, controls to pile up, under the conditions of temperature is 25-35 DEG C, the 23-that becomes thoroughly decomposed 26d;Pile up intensification of becoming thoroughly decomposed afterwards so that temperature reaches 60-70 DEG C, maintain 2-3d, prepare the straw-excrement composting become thoroughly decomposed;
(3) preparation of biological organic fertilizer
Above-mentioned prepared straw-night soil is carried out airing so that it is after temperature is down to 30-35 DEG C, be uniformly blended into colloid Gemma bacillus agent, and add carbamide, magnesium sulfate and activated carbon and Borax, it is sufficiently mixed fermentation 2-3d;Add after having fermented Control temperature 25 DEG C after entering saccharomyces cerevisiae microbial inoculum and aspergillus niger microbial inoculum mix homogeneously again to ferment 2-3d;By the mixture after fermentation It is dried, measures pack, obtain bio-organic fertilizer.
Preferably, consisting of of described saccharomyces cerevisiae microbial inoculum fluid medium: 20g peptone, 10g yeast extract, 2g glucose, appropriate distilled water dissolves, adds 15ml 0.2% adenine solution, constant volume to 1L;120 DEG C of autoclavings 15min。
Preferably, consisting of of the fluid medium of described colloid bacillus cereus microbial inoculum: sucrose 10g, yeast extract 0.3g, ammonium sulfate 0.5g, calcium carbonate 0.5g, Magnesium sulfate heptahydrate 0.5g, dipotassium hydrogen phosphate 1g, adjust pH value 7.0-7.5 constant volume To 1L.
Preferably, the composition of described inorganic salt solution is: NaCl 1 part, CaCl20.5 part, KCl 0.5 part, K2HPO4 0.5 part, NaHCO30.25 part, MgSO41 part, FeSO4·7H2O0.025 part, distilled water 2000 parts;Described dispensing mixing For: 2000 parts of wheat bran, soybean cake powder 2000 parts, 1000 parts of peanut straw powder.
Compared with prior art the invention have the benefit that bio-organic fertilizer prepared by the present invention organic >= 60%, living bacteria count >=0.5 hundred million/g, excrement colibacillus group number≤30/g.Compared with common chemical fertilizer (100kg/ mu), it is possible to decrease Soil valence heavy metal ions content more than 8%;Reduce crop heavy metal enrichment more than 10%;Reduction especially for Cr Reach more than 70%;Make crop yield more than 5%, and there is the multiple efficacies such as water conservation, fertilizer conservation and improvement Soil structure.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.Following example are used for The present invention is described, but is not limited to the scope of the present invention.
The preparation method of the bio-organic fertilizer of a kind of repairing heavy metal in soil, comprises the following steps:
(1) prepared by microbial bacterial agent
The preparation method of saccharomyces cerevisiae microbial inoculum: take saccharomyces cerevisiae strain 20 parts, the water termostat adding 30 DEG C of five times of quality stirs Mixing 0.5h until being completely dissolved, obtained liquid adds in the fluid medium of 1L volume, detects cell density after cultivating 5d Reach 2.2 × 109cfu/mL。
The ingredient of fluid medium is: 20g peptone, 10g yeast extract, 2g glucose, appropriate distilled water is molten Solve, add 15ml 0.2% adenine solution, constant volume to 1L;120 DEG C of autoclaving 15min.
The preparation method of colloid bacillus cereus microbial inoculum: take colloid bacillus cereus strain 20 parts, adds 35 DEG C of five times of quality Water termostat stirring 0.5h is until being completely dissolved;Obtained liquid adds in the fluid medium of 1L volume, and 150rpm, at 32 DEG C Isothermal vibration cultivates 6d so that cell density reaches 7.8 × 109cfu/mL。
Consisting of of fluid medium: sucrose 10g, yeast extract 0.3g, ammonium sulfate 0.5g, calcium carbonate 0.5g, seven water sulfur Acid magnesium 0.5g, dipotassium hydrogen phosphate 1g, adjust pH value 7.0-7.5 constant volume to 1L.
The preparation method of aspergillus niger microbial inoculum: take aspergillus niger strain 20 parts, adds the water termostat stirring of 30 DEG C of five times of quality 0.5h, until being completely dissolved, joins obtained liquid in inorganic salt solution, by inorganic salt solution (NaCl 1g, CaCl2 0.5g, KCl 0.5g, K2HPO40.5g, NaHCO30.25g, MgSO41g, FeSO4·7H2O 25mg, distilled water 2L) and 5kg Dispensing mixing (wheat bran: soybean cake powder: peanut straw powder=2:2:1) is stirred inoculation.I.e. obtain black at 28 DEG C after ferment at constant temperature 5d Aspergillosis microbial inoculum.
(2) straw-excrement composting becomes thoroughly decomposed
Taking crushed stalk is that diameter is less than the unqualified of 2mm, adds changing food waste mixing, adds decomposing agent and animal dung Just, keeping moisture at 60%-70%, controlling compost height is 1.5 meters, controls to pile up, under the conditions of temperature is 30 DEG C, the 25d that becomes thoroughly decomposed;It Rear accumulation is become thoroughly decomposed intensification so that temperature reaches 60-70 DEG C, maintains 2d, prepares the straw-excrement composting become thoroughly decomposed;
(3) preparation of biological organic fertilizer
Above-mentioned prepared straw-night soil is carried out airing so that it is after temperature is down to 30 DEG C, be uniformly blended into colloid spore Bacillus microbial inoculum, and add the activated carbon of carbamide, magnesium sulfate and 0.5% and the Borax of 0.1%, it is sufficiently mixed fermentation 2d;Fermentation Control temperature 25 DEG C after adding the saccharomyces cerevisiae microbial inoculum of 1% and the aspergillus niger microbial inoculum mix homogeneously of 1% after completing again to ferment 3d; Mixture after fermentation is dried, measures pack, obtains bio-organic fertilizer.
The bio-organic fertilizer of described repairing heavy metal in soil, including the composition of following percentage by weight:
Described straw-night soil includes following main component:
Described feces of livestock and poultry is chicken manure and cattle manure mixed in equal amounts forms.
50% described straw includes 20% corn straw, 20% rice straw, 10% wheat stalk.
In each embodiment, the composition of each percentage by weight of bio-organic fertilizer please see table:
In each embodiment, the composition of each percentage by weight of straw-night soil please see table:
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Embodiment 5 Embodiment 6 Embodiment 7
Straw 50 40 45 55 60 65 45
Decomposing agent 1 0.8 0.9 1.5 1.2 1.5 1
Changing food waste 5 3 5 8 7 6 5
Feces of livestock and poultry Surplus Surplus Surplus Surplus Surplus Surplus Surplus
The bio-organic fertilizer preparing the present invention carries out test and obtains following data:
By the fertilizer application of the present invention in Rice Cropping, after testing 1 year heavy metal treatment effect and with common compound fertilizer (N:P2O5:K2O=2:1:3) contrast, obtain following data:
Dose: 100kg/ mu, it is possible to decrease soil valence heavy metal ions content 8.5-10%;Reduce crop heavy metal Enrichment 12-15%;Reduction for Cr reaches 75-80%;Make crop yield 6-8%.
It is below comparative example, the flora in the fertilizer in the present invention is made a concrete analysis of.
Comparative example 1
Colloid bacillus cereus microbial inoculum in embodiment 1 is removed, heavy metal treatment effect with common after testing a year Compound fertilizer (N:P2O5:K2O=2:1:3) contrast, obtain following data:
Dose: 100kg/ mu, it is possible to decrease soil valence heavy metal ions content 2-3%;Reduce crop heavy metal rich Collection 3-5%;Reduction for Cr reaches 25-30%;Make crop yield 4-5%.
Comparative example 2
Saccharomyces cerevisiae microbial inoculum in embodiment 1 is removed, heavy metal treatment effect being combined with common after test 1 year Fertile (N:P2O5:K2O=2:1:3) contrast, obtain following data:
Dose: 100kg/ mu, it is possible to decrease soil valence heavy metal ions content 2-3%;Reduce crop heavy metal rich Collection 2-3%;Reduction for Cr reaches 55-60%;Make crop yield 5-6%.
Comparative example 3
By in embodiment 1 aspergillus niger microbial inoculum remove, after testing 1 year heavy metal treatment effect and with common compound fertilizer (N:P2O5:K2O=2:1:3) contrast, obtain following data:
Dose: 100kg/ mu, it is possible to decrease soil valence heavy metal ions content 3-4%;Reduce crop heavy metal rich Collection 6-8%;Reduction for Cr reaches 45-50%;Make crop yield 3-5%.
Comparative example 4
Aspergillus niger microbial inoculum in embodiment 1 and saccharomyces cerevisiae microbial inoculum being removed, after testing 1 year, heavy metal treatment effect is also With common compound fertilizer (N:P2O5:K2O=2:1:3) contrast, obtain following data:
Dose: 100kg/ mu, it is possible to decrease soil valence heavy metal ions content 1-2%;Reduce crop heavy metal rich Collection 2-3%;Reduction for Cr reaches 20-30%;Make crop yield 3-5%.
It is used alone one it is recognised that three kinds of microorganisms are used in conjunction with ratio by the test data of comparative example 1-4 The heavy metal-polluted soil repairing effect that microorganism or two kinds of microorganisms are used in conjunction with is the most a lot.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For Yuan, on the premise of without departing from the technology of the present invention principle, it is also possible to make some improvement and modification, these improve and modification Also should be regarded as protection scope of the present invention.

Claims (7)

1. the bio-organic fertilizer of a repairing heavy metal in soil, it is characterised in that include the composition of following percentage by weight:
Colloid bacillus cereus microbial inoculum 1-3%
Carbamide 0.2-0.7%
Magnesium sulfate 0.05-0.2%
Activated carbon 0.3-0.8%
Borax 0.08-0.15%
Saccharomyces cerevisiae microbial inoculum 0.5-1.5%
Aspergillus niger microbial inoculum 0.7-2%.
Straw-night soil surplus.
The bio-organic fertilizer of a kind of repairing heavy metal in soil the most as claimed in claim 1, it is characterised in that described straw Stalk-night soil includes following main component:
Straw 40-65%
Decomposing agent 0.8-1.5%
Changing food waste 3-8%
Feces of livestock and poultry surplus.
The bio-organic fertilizer of a kind of repairing heavy metal in soil the most as claimed in claim 1, it is characterised in that described poultry Feces is chicken manure and cattle manure mixed in equal amounts forms.
4. the preparation method of the bio-organic fertilizer of a repairing heavy metal in soil, it is characterised in that comprise the following steps:
(1) preparation of microbial bacterial agent
A, the preparation method of saccharomyces cerevisiae microbial inoculum: take saccharomyces cerevisiae strain, add constant temperature in 30 DEG C of water and stir until being completely dissolved, Obtained liquid adds in fluid medium, detects cell density and reach 1.5-3.1 × 10 after cultivating 4-6d9Cfu/mL, i.e. Obtain saccharomyces cerevisiae microbial inoculum;
B, the preparation method of colloid bacillus cereus microbial inoculum: take colloid bacillus cereus strain, be added to the water constant temperature and stir to the most molten Solve;Obtained liquid adds in fluid medium, and at 150rpm, 30-35 DEG C, isothermal vibration cultivates 4-6d so that cell density Reach 6.2-8.3 × 109Cfu/mL, obtains colloid bacillus cereus microbial inoculum;
C, the preparation method of aspergillus niger microbial inoculum: take aspergillus niger strain, be added to the water constant temperature and stir until being completely dissolved, obtained Liquid join in inorganic salt solution, inorganic salt solution is mixed with dispensing, be stirred inoculation, at 25-30 DEG C, constant temperature is sent out Aspergillus niger microbial inoculum is i.e. obtained after ferment 4-6d;
(2) straw-excrement composting becomes thoroughly decomposed
Taking crushed stalk is that diameter is less than the unqualified of 2mm, adds changing food waste mixing, adds decomposing agent and feces of livestock and poultry, protects Water holding divides at 60%-70%, and controlling compost height is 1.5 meters, controls to pile up, under the conditions of temperature is 25-35 DEG C, the 23-26d that becomes thoroughly decomposed; Pile up intensification of becoming thoroughly decomposed afterwards so that temperature reaches 60-70 DEG C, maintain 2-3d, prepare the straw-excrement composting become thoroughly decomposed;
(3) preparation of biological organic fertilizer
Above-mentioned prepared straw-night soil is carried out airing so that it is after temperature is down to 30-35 DEG C, be uniformly blended into colloid spore Bacillus microbial inoculum, and add carbamide, magnesium sulfate and activated carbon and Borax, it is sufficiently mixed fermentation 2-3d;Add after having fermented and make Control temperature 25 DEG C after brewer yeast microbial inoculum and aspergillus niger microbial inoculum mix homogeneously again to ferment 2-3d;Mixture after fermentation is carried out Be dried, metering packs, and obtains bio-organic fertilizer.
The preparation method of the bio-organic fertilizer of a kind of repairing heavy metal in soil the most as claimed in claim 4, it is characterised in that Consisting of of described saccharomyces cerevisiae microbial inoculum fluid medium: 20g peptone, 10g yeast extract, 2g glucose, the most double Steam water dissolution, add 15ml0.2% adenine solution, constant volume to 1L;120 DEG C of autoclaving 15min.
The preparation method of the bio-organic fertilizer of a kind of repairing heavy metal in soil the most as claimed in claim 4, it is characterised in that Consisting of of the fluid medium of described colloid bacillus cereus microbial inoculum: sucrose 10g, yeast extract 0.3g, ammonium sulfate 0.5g, carbon Acid calcium 0.5g, Magnesium sulfate heptahydrate 0.5g, dipotassium hydrogen phosphate 1g, adjust pH value 7.0-7.5 constant volume to 1L.
The preparation method of the bio-organic fertilizer of a kind of repairing heavy metal in soil the most as claimed in claim 4, it is characterised in that The composition of described inorganic salt solution is: NaCl 1 part, CaCl20.5 part, KCl 0.5 part, K2HPO40.5 part, NaHCO3 0.25 part, MgSO41 part, FeSO4·7H2O 0.025 part, distilled water 2000 parts;Described dispensing is mixed into: 2000 parts of wheat bran, Soybean cake powder 2000 parts, 1000 parts of peanut straw powder.
CN201610458389.3A 2016-06-22 2016-06-22 Biological organic fertilizer for repairing soil heavy metal and preparation method thereof Active CN106116946B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610458389.3A CN106116946B (en) 2016-06-22 2016-06-22 Biological organic fertilizer for repairing soil heavy metal and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610458389.3A CN106116946B (en) 2016-06-22 2016-06-22 Biological organic fertilizer for repairing soil heavy metal and preparation method thereof

Publications (2)

Publication Number Publication Date
CN106116946A true CN106116946A (en) 2016-11-16
CN106116946B CN106116946B (en) 2020-10-27

Family

ID=57267957

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610458389.3A Active CN106116946B (en) 2016-06-22 2016-06-22 Biological organic fertilizer for repairing soil heavy metal and preparation method thereof

Country Status (1)

Country Link
CN (1) CN106116946B (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106591378A (en) * 2016-12-26 2017-04-26 北京大学深圳研究生院 Method for carrying out anaerobic digestion by using heavy metal ions-rich biomass waste materials
CN107056492A (en) * 2017-05-31 2017-08-18 安徽永志环能科技有限公司 Suitable for the leavening agent for poultry excrement and its application method of cold district
CN107739280A (en) * 2017-11-15 2018-02-27 李永锋 A kind of bio-feritlizer of reduction Cr pollutions
CN107827382A (en) * 2017-11-16 2018-03-23 广东弘朝科技有限公司 A kind of hexavalent chrome reduction agent and preparation method thereof
CN107827645A (en) * 2017-11-15 2018-03-23 李永锋 A kind of bio-feritlizer of reduction Cd pollutions
CN108043873A (en) * 2017-12-25 2018-05-18 谢涛 A kind of heavy metal-polluted soil biological fixation agent and its application process
CN108076948A (en) * 2017-12-20 2018-05-29 中国计量大学 A kind of method that sodium acid carbonate is applied in the soil of highly acid tea place and reduces plumbi content of tea
CN109912345A (en) * 2019-04-02 2019-06-21 北京国达恒泰科贸有限责任公司 Solid organic castoff is the function organic compound fertilizer and preparation method of substrate and application
CN109988020A (en) * 2019-04-15 2019-07-09 深圳绿欣农业环保科技有限公司 Aquaculture and planting industry cyclic utilization of waste method
CN110937952A (en) * 2019-10-28 2020-03-31 安徽省通源环境节能股份有限公司 Preparation method of ecological restoration matrix for heavy metal contaminated soil
CN113511926A (en) * 2021-04-28 2021-10-19 新至农业生态科技有限公司 Method for comprehensively utilizing livestock and poultry manure resources and returning to field
CN117185873A (en) * 2023-09-20 2023-12-08 中国科学院沈阳应用生态研究所 Fertilizer for improving soil productivity and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1054362C (en) * 1996-04-29 2000-07-12 刘文治 Method for producing organic and inorganic mixed microbe fertilizer
CN103495602A (en) * 2013-09-30 2014-01-08 河南金谷实业发展有限公司 Method for restoring hexavalent chromium-polluted soil by utilizing microbial fermentation
CN104761410A (en) * 2014-01-06 2015-07-08 英卓科技有限公司 Preparation method of humic acid-containing fertilizer and soil conditioner composition
CN104788263A (en) * 2015-04-16 2015-07-22 王龙潮 Composite soil conditioner

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1054362C (en) * 1996-04-29 2000-07-12 刘文治 Method for producing organic and inorganic mixed microbe fertilizer
CN103495602A (en) * 2013-09-30 2014-01-08 河南金谷实业发展有限公司 Method for restoring hexavalent chromium-polluted soil by utilizing microbial fermentation
CN104761410A (en) * 2014-01-06 2015-07-08 英卓科技有限公司 Preparation method of humic acid-containing fertilizer and soil conditioner composition
CN104788263A (en) * 2015-04-16 2015-07-22 王龙潮 Composite soil conditioner

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
卢中华 主编: "《微生物学及免疫学基础》", 30 September 1991, 北京:中国科学技术出版社 *
常学秀 等: "《环境污染微生物学》", 30 November 2006, 北京:高等教育出版社 *
李登煜 等: "《农业微生物应用技术》", 30 November 2001, 成都:四川大学出版社 *
詹益兴 主编: "《化工小商品生产法(第十集)》", 28 February 1993, 湖南科学技术出版社 *
鞠美庭 等: "《生物质固废资源化技术手册》", 31 March 2014, 天津:天津大学出版社 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106591378A (en) * 2016-12-26 2017-04-26 北京大学深圳研究生院 Method for carrying out anaerobic digestion by using heavy metal ions-rich biomass waste materials
CN107056492A (en) * 2017-05-31 2017-08-18 安徽永志环能科技有限公司 Suitable for the leavening agent for poultry excrement and its application method of cold district
CN107739280A (en) * 2017-11-15 2018-02-27 李永锋 A kind of bio-feritlizer of reduction Cr pollutions
CN107827645A (en) * 2017-11-15 2018-03-23 李永锋 A kind of bio-feritlizer of reduction Cd pollutions
CN107827382A (en) * 2017-11-16 2018-03-23 广东弘朝科技有限公司 A kind of hexavalent chrome reduction agent and preparation method thereof
CN108076948A (en) * 2017-12-20 2018-05-29 中国计量大学 A kind of method that sodium acid carbonate is applied in the soil of highly acid tea place and reduces plumbi content of tea
CN108043873A (en) * 2017-12-25 2018-05-18 谢涛 A kind of heavy metal-polluted soil biological fixation agent and its application process
CN109912345A (en) * 2019-04-02 2019-06-21 北京国达恒泰科贸有限责任公司 Solid organic castoff is the function organic compound fertilizer and preparation method of substrate and application
CN109988020A (en) * 2019-04-15 2019-07-09 深圳绿欣农业环保科技有限公司 Aquaculture and planting industry cyclic utilization of waste method
CN110937952A (en) * 2019-10-28 2020-03-31 安徽省通源环境节能股份有限公司 Preparation method of ecological restoration matrix for heavy metal contaminated soil
CN113511926A (en) * 2021-04-28 2021-10-19 新至农业生态科技有限公司 Method for comprehensively utilizing livestock and poultry manure resources and returning to field
CN117185873A (en) * 2023-09-20 2023-12-08 中国科学院沈阳应用生态研究所 Fertilizer for improving soil productivity and preparation method thereof

Also Published As

Publication number Publication date
CN106116946B (en) 2020-10-27

Similar Documents

Publication Publication Date Title
CN106116946A (en) A kind of bio-organic fertilizer of repairing heavy metal in soil and preparation method thereof
US20190300407A1 (en) Biological Floc and Uses Thereof, for example, in Sludge Innocent Treatment and Industrial Water Purification Treatment
CN103495602B (en) Method for restoring hexavalent chromium-polluted soil by utilizing microbial fermentation
CN106754461B (en) Biological floc and preparation method and application thereof
CN101333123B (en) Process for reclaiming city domestic sludge
CN101983952B (en) Method for producing organic fertilizer by using municipal sewage sludge
Aguilar-Paredes et al. Microbial community in the composting process and its positive impact on the soil biota in sustainable agriculture
CN103319214B (en) Municipal sludge is utilized to manufacture method and the special leaven thereof of biological organic fertilizer
CN106986720A (en) The method for producing soil conditioner using the waste residue for handling livestock breeding wastewater generation
CN103694010B (en) A kind of Ultrahigh-temperaturaerobic aerobic fermentation method for sludge and application thereof
CN105838644A (en) Compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil
CN102731177B (en) Two-step bioremediation method of organic pollutants in compost organic raw materials
CN104263374B (en) A kind of soil heavy metal combined stabilizer and its application method
CN104450597A (en) Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant
CN101244955A (en) Biological fertilizer and manufacture method thereof
CN105695354A (en) Process and application for treating municipal sewage sludge by means of ultrahigh-temperature aerobic composting fermentation
CN106278417A (en) A kind of method utilizing municipal sludge to produce biological organic fertilizer
CN102815977A (en) Process for producing organic fertilizer by livestock and poultry manure composting
CN105565959A (en) Soil ecological system restoring organic fertilizer
CN105061004A (en) Preparation method for biological organic fertilizer
CN109796113A (en) A kind of sludge and derivative manufacturing method of high speed processing moisture content 80 percent
CN113773987A (en) Biological agent for improving aerobic fermentation efficiency of organic waste and preparation method thereof
Mucha et al. Re-use of digestate and recovery techniques
CN113773986B (en) Microbial inoculum for rapid temperature rise of organic solid waste aerobic fermentation in winter and preparation method thereof
CN104311177A (en) Method of preparing bio-organic fertilizer by utilizing papermaking sludge and tradtional Chinese medicine residues

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210426

Address after: 611830 no.615, Yanhua Road, Sichuan Dujiangyan Economic Development Zone, Dujiangyan City, Chengdu City, Sichuan Province

Patentee after: CHENGDU HUAHONG BIOTECHNOLOGY Co.,Ltd.

Address before: 610039 No. 999, golden week Road, Chengdu, Sichuan, Jinniu District

Patentee before: XIHUA University

Patentee before: CHENGDU HUAHONG BIOTECHNOLOGY Co.,Ltd.