CN106084081B - The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus - Google Patents
The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus Download PDFInfo
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- CN106084081B CN106084081B CN201610426470.3A CN201610426470A CN106084081B CN 106084081 B CN106084081 B CN 106084081B CN 201610426470 A CN201610426470 A CN 201610426470A CN 106084081 B CN106084081 B CN 106084081B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The invention discloses a kind of preparation methods of polysaccharide component in Daxing'an Mountainrange urtica angustifolia and birch Phellinus comprising following steps：Pretreatment；Extracting solution is prepared, is ultrasonically treated, obtains sonification fluid；Complex enzyme is added, obtains enzymolysis liquid；Microwave treatment obtains microwave treatment liquid；Centrifuge is centrifuged, and the supernatant after centrifugation is taken；Ethyl alcohol, and 10 20h of alcohol precipitation are added in supernatant；Centrifugation, precipitation；Precipitation is dissolved in a small amount of water, freeze-drying, the extraction cost of polysaccharide component can be reduced by obtaining the polysaccharide component present invention, improve recovery rate, use the extracting method of the present invention, recovery rate can be higher than 75 mg/g, and this method is easily controllable, easy to operate, and the method being combined using ultrasonic wave auxiliary, microwave radiation technology, extraction efficiency is substantially increased, safe, quick, energy saving, extraction time is short.
Description
Technical field
The invention belongs to extracting method technical fields, more particularly, in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus
The preparation method and products application of polysaccharide component, belong to field of natural organic chemistry.
Background technology
In Daxing'an Mountainrange, containing a large amount of urtica angustifolia and birch Phellinus, urtica angustifolia be a common ethnic drug and
Folk medicine, first recorded in《Figure is through book on Chinese herbal medicine》, there is dispelling wind and removing obstruction in the meridians, soothing the liver arresting convulsion, disperse accumulation defaecation, removing toxic substances and other effects, urtica angustifolia
Nineteen ninety-five is taken in《The Sanitation Ministry medicine standard》(Tibetan medicine) first.In foreign countries, urtica angustifolia since the cist generation just
It is medicinal, treatment rheumatic illness and some geriatric diseases are widely used in, significant in efficacy, polysaccharide is anti-inflammatory, anticancer main
One of active constituent.Pharmacological research also confirms that polysaccharide from urtica fissa truly has significant anti-inflammatory, enhancing immunization to grind both at home and abroad in recent years
Study carefully and shows that polysaccharide has important activity effect, and birch in antitumor, immunological regulation, anti-aging, hypoglycemic, antiviral etc.
It is a kind of traditional Chinese medicine in China to set Phellinus, belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, phellinus
Belong to, main parasitic is on the trunks such as mulberry tree, willow, robur.It is many at present research shows that, that is extracted in Phellinus fructification is more
Sugared ingredient has apparent antitumaous effect, while improving the functions such as immunity of organisms.
Therefore, the polysaccharide component in urtica angustifolia and birch Phellinus how is extracted, for urtica angustifolia and birch Phellinus
It utilizes, has a very important role and meaning, the development for drug have good impetus.
Invention content
Present invention problem in view of the prior art provides polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus
Preparation method and products application, can reduce the extraction cost of polysaccharide component, improve recovery rate, use the extraction side of the present invention
Method, recovery rate can be higher than 75 mg/g, and this method is easily controllable, easy to operate, and utilize ultrasonic wave auxiliary, microwave radiation technology phase
In conjunction with method, substantially increase extraction efficiency, safe, quick, energy saving, extraction time is short.
To achieve the above object, the present invention provides the following technical solutions：A kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus
The preparation method of middle polysaccharide component, which is characterized in that it includes the following steps：
（1）The pretreatment of urtica angustifolia and birch Phellinus raw material；
（2）Extracting solution is prepared, and using being ultrasonically treated, obtains sonification fluid；
（3）In the step（2）Complex enzyme is added in the sonification fluid of preparation, obtains enzymolysis liquid；
（4）By the step（3）Obtained enzymolysis liquid carries out microwave treatment, obtains microwave treatment liquid；
（5）Microwave treatment liquid is centrifuged using centrifuge, takes the supernatant after centrifugation；
（6）Ethyl alcohol, and alcohol precipitation 10-20h are added in supernatant；
（7）To the step（6）In precipitation solution centrifuged, precipitate；
（8）Precipitation is dissolved in a small amount of water, is freeze-dried, obtains polysaccharide component.
Further, preferably, the step（1）In, the urtica angustifolia and birch Phellinus of certain mass are weighed, clear water is used
It is cleaned, is dried at 65 DEG C later, then, ground using Chinese herbal medicine grinding and crushing machine, raw material powder is made,
It is sieved for subsequent use to raw material powder using 50 mesh sieve；The step（2）In, when preparing extracting solution, by solid-liquid ratio 1:The amount of 40g/ml
Deionized water is added into raw material powder, is uniformly mixing to obtain extracting solution, then, using the ultrasonic wave of 600W power to extracting solution
Supersound process 20min is carried out, and it is 40-45 DEG C to keep the temperature of extracting solution.
Further, preferably, the step（3）In, cellulase, pectase and albumen are included at least in complex enzyme
Enzyme, and the ratio of cellulase, pectase and protease is 1:1:1, after protease is added, it is 50 to keep the temperature of enzymolysis liquid
DEG C, PH 4.5, enzymolysis time 150min.
Further, preferably, the step（4）In, when microwave treatment, the power of microwave is 650W, microwave treatment
Temperature is 55-60 DEG C, microwave treatment time 2-3min, and require to carry out it at interval of 5min microwave treatment it is primary, at least
Processing 4 times.
Further, preferably, in the step（5）In, when being centrifuged using centrifuge, the rotating speed of centrifugation is
5000r/min。
Further, preferably, the step（6）In, the ethyl alcohol being added in supernatant is the second that mass fraction is 92%
Alcohol is sealed after addition, is placed in 5 DEG C of water-bath, after keeping the temperature 20h, filters alcohol precipitation.
Further, preferably, the step（7）In, when being centrifuged to precipitation solution, centrifugal rotational speed 4000r/min.
Further, the present invention provides a kind of preparation methods of polysaccharide component in Daxing'an Mountainrange urtica angustifolia and birch Phellinus
The application of polysaccharide component obtained, it is characterised in that：It is used to prepare anti-oxidation medicine, anti-fatigue medicament, anti-tumor drug
Or hypertension drug.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention can reduce the extraction cost of polysaccharide component, improve recovery rate, use the extracting method of the present invention, extraction
Rate can be higher than 75 m g/g, and this method is easily controllable, easy to operate, and be combined using ultrasonic wave auxiliary, microwave radiation technology
Method substantially increases extraction efficiency, and safe, quick, energy saving, extraction time is short.
Description of the drawings
Fig. 1 is the flowage structure schematic diagram of the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution：Polysaccharide in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus
The preparation method of ingredient, which is characterized in that it includes the following steps：
（1）The pretreatment of urtica angustifolia and birch Phellinus raw material：The urtica angustifolia and birch Phellinus of certain mass are weighed, is used
Clear water is cleaned, and is dried at 65 DEG C later, then, is ground using Chinese herbal medicine grinding and crushing machine, and raw material is made
Powder sieves for subsequent use to raw material powder using 50 mesh sieve；
（2）Extracting solution is prepared, and using being ultrasonically treated, obtains sonification fluid：When preparing extracting solution, by solid-liquid ratio 1:
Deionized water is added into raw material powder for the amount of 40g/ml, is uniformly mixing to obtain extracting solution, then, using the ultrasound of 600W power
Wave carries out supersound process 20min to extracting solution, and it is 40-45 DEG C to keep the temperature of extracting solution；
（3）In the step（2）Complex enzyme is added in the sonification fluid of preparation, obtains enzymolysis liquid：In complex enzyme at least
Including cellulase, pectase and protease, and the ratio of cellulase, pectase and protease is 1:1:1, protease is added
Afterwards, it is 50 DEG C, PH 4.5, enzymolysis time 150min to keep the temperature of enzymolysis liquid；
（4）By the step（3）Obtained enzymolysis liquid carries out microwave treatment, obtains microwave treatment liquid：It is micro- when microwave treatment
The power of wave is 650W, and the temperature of microwave treatment is 55-60 DEG C, microwave treatment time 2-3min, and is required at interval of 5min
It is primary that microwave treatment is carried out to it, is at least handled 4 times；
（5）Microwave treatment liquid is centrifuged using centrifuge, takes the supernatant after centrifugation：Using centrifuge carry out from
When the heart, the rotating speed of centrifugation is 5000r/min；
（6）Ethyl alcohol, and alcohol precipitation 10-20h are added in supernatant：The ethyl alcohol being added in supernatant is that mass fraction is
92% ethyl alcohol is sealed after addition, is placed in 5 DEG C of water-bath, after keeping the temperature 20h, filters alcohol precipitation；
（7）To the step（6）In precipitation solution centrifuged, precipitate：When being centrifuged to precipitation solution, centrifugal rotational speed is
4000r/min；
（8）Precipitation is dissolved in a small amount of water, is freeze-dried, obtains polysaccharide component.
The present invention also provides a kind of preparation methods of polysaccharide component in Daxing'an Mountainrange urtica angustifolia and birch Phellinus to be made
Polysaccharide component application, it is characterised in that：It is used to prepare anti-oxidation medicine, anti-fatigue medicament, anti-tumor drug or
Hypertension drug.
The present invention can reduce the extraction cost of polysaccharide component, improve recovery rate, use the extracting method of the present invention, extraction
Rate can be higher than 75 m g/g, and this method is easily controllable, easy to operate, and be combined using ultrasonic wave auxiliary, microwave radiation technology
Method substantially increases extraction efficiency, and safe, quick, energy saving, extraction time is short.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus, which is characterized in that it include with
Lower step：
（1）The pretreatment of urtica angustifolia and birch Phellinus raw material；
（2）Extracting solution is prepared, and using being ultrasonically treated, obtains sonification fluid；
（3）In the step（2）Complex enzyme is added in the sonification fluid of preparation, obtains enzymolysis liquid；
（4）By the step（3）Obtained enzymolysis liquid carries out microwave treatment, obtains microwave treatment liquid；
（5）Microwave treatment liquid is centrifuged using centrifuge, takes the supernatant after centrifugation；
（6）Ethyl alcohol, and alcohol precipitation 10-20h are added in supernatant；
（7）To the step（6）In precipitation solution centrifuged, precipitate；
（8）Precipitation is dissolved in a small amount of water, is freeze-dried, obtains polysaccharide component;
The step（1）In, the urtica angustifolia and birch Phellinus of certain mass are weighed, is cleaned with clear water, later at 65 DEG C
Then lower drying is ground using Chinese herbal medicine grinding and crushing machine, raw material powder is made, and is sieved to raw material powder using 50 mesh
It sieves for subsequent use at end；The step（2）In, when preparing extracting solution, by solid-liquid ratio 1:The amount of 40g/ml is added into raw material powder and goes
Ionized water is uniformly mixing to obtain extracting solution, then, supersound process 20min is carried out to extracting solution using the ultrasonic wave of 600W power,
And it is 40-45 DEG C to keep the temperature of extracting solution;
The step（3）In, in complex enzyme include at least cellulase, pectase and protease, and cellulase, pectase and
The ratio of protease is 1:1:1, after protease is added, it is 50 DEG C, PH 4.5 to keep the temperature of enzymolysis liquid, and enzymolysis time is
150min。
2. the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia according to claim 1 and birch Phellinus,
It is characterized in that：The step（4）In, when microwave treatment, the power of microwave is 650W, and the temperature of microwave treatment is 55-60 DEG C,
Microwave treatment time is 2-3min, and requires to carry out it at interval of 5min microwave treatment primary, is at least handled 4 times.
3. the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia according to claim 1 and birch Phellinus,
It is characterized in that：In the step（5）In, when being centrifuged using centrifuge, the rotating speed of centrifugation is 5000r/min.
4. the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia according to claim 1 and birch Phellinus,
It is characterized in that：The step（6）In, the ethyl alcohol being added in supernatant is the ethyl alcohol that mass fraction is 92%, is carried out after addition
Sealing, is placed in 5 DEG C of water-bath, after keeping the temperature 20h, filters alcohol precipitation.
5. the preparation method of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia according to claim 1 and birch Phellinus,
It is characterized in that：The step（7）In, when being centrifuged to precipitation solution, centrifugal rotational speed 4000r/min.
6. polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus according to claim 1-5 any one
The application of polysaccharide component made from preparation method, it is characterised in that：It is used to prepare anti-oxidation medicine, anti-fatigue medicament, resists and swell
The drug or hypertension drug of tumor.
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| CN201610426470.3A CN106084081B (en) | 2016-06-16 | 2016-06-16 | The preparation method and products application of polysaccharide component in a kind of Daxing'an Mountainrange urtica angustifolia and birch Phellinus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580552A (en) * | 2009-06-24 | 2009-11-18 | 张海悦 | Preparation method of urtica angustifolia polysaccharide and application thereof |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
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| KR100663712B1 (en) * | 2005-05-24 | 2007-01-03 | (주)새롬바이오 | A crude exopolysaccharides produced from Phellinus baumii mycellium having hypoglycemic activity and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101580552A (en) * | 2009-06-24 | 2009-11-18 | 张海悦 | Preparation method of urtica angustifolia polysaccharide and application thereof |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN104987428A (en) * | 2015-07-23 | 2015-10-21 | 中国科学院西北高原生物研究所 | Compound enzyme-microwave extraction method for optimizing Nitraria polysaccharide by response surface methodology |
Non-Patent Citations (1)
| Title |
|---|
| 超声波复合酶法提取桑黄多糖研究;谢丽源等;《食品科学》;20100515;第31卷(第10期);第81-85页 * |
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